LOCUS       NM_000662               1799 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens N-acetyltransferase 1 (NAT1), transcript variant 5,
            mRNA.
ACCESSION   NM_000662 XM_938769
VERSION     NM_000662.8
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Gu,W., Li,C., Shen,T., Tong,L., Yuan,W., Zheng,X., Wang,T.,
            Wang,S., Zhu,B., Zhang,C. and Zhang,C.
  TITLE     NAT1 inhibits liver metastasis of colorectal cancer by regulating
            EMT and glycolysis
  JOURNAL   Aging (Albany NY) 16 (12), 10546-10562 (2024)
   PUBMED   38916406
  REMARK    GeneRIF: NAT1 inhibits liver metastasis of colorectal cancer by
            regulating EMT and glycolysis.
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Minchin,R.F., Hanna,P.E., Dupret,J.M., Wagner,C.R.,
            Rodrigues-Lima,F. and Butcher,N.J.
  TITLE     Arylamine N-acetyltransferase I
  JOURNAL   Int J Biochem Cell Biol 39 (11), 1999-2005 (2007)
   PUBMED   17392017
REFERENCE   3  (bases 1 to 1799)
  AUTHORS   Barker,D.F., Husain,A., Neale,J.R., Martini,B.D., Zhang,X.,
            Doll,M.A., States,J.C. and Hein,D.W.
  TITLE     Functional properties of an alternative, tissue-specific promoter
            for human arylamine N-acetyltransferase 1
  JOURNAL   Pharmacogenet Genomics 16 (7), 515-525 (2006)
   PUBMED   16788383
REFERENCE   4  (bases 1 to 1799)
  AUTHORS   Boukouvala,S. and Sim,E.
  TITLE     Structural analysis of the genes for human arylamine
            N-acetyltransferases and characterisation of alternative
            transcripts
  JOURNAL   Basic Clin Pharmacol Toxicol 96 (5), 343-351 (2005)
   PUBMED   15853926
  REMARK    GeneRIF: Exon-intron structure and tissue localization of the human
            NAT1 gene is described.
REFERENCE   5  (bases 1 to 1799)
  AUTHORS   Butcher,N.J., Arulpragasam,A., Goh,H.L., Davey,T. and Minchin,R.F.
  TITLE     Genomic organization of human arylamine N-acetyltransferase Type I
            reveals alternative promoters that generate different 5'-UTR splice
            variants with altered translational activities
  JOURNAL   Biochem J 387 (Pt 1), 119-127 (2005)
   PUBMED   15487985
REFERENCE   6  (bases 1 to 1799)
  AUTHORS   Husain,A., Barker,D.F., States,J.C., Doll,M.A. and Hein,D.W.
  TITLE     Identification of the major promoter and non-coding exons of the
            human arylamine N-acetyltransferase 1 gene (NAT1)
  JOURNAL   Pharmacogenetics 14 (7), 397-406 (2004)
   PUBMED   15226672
  REMARK    GeneRIF: In breast, NAT1 mRNA is transcribed from a strong promoter
            located 11.8 kb upstream of the translated exon, and the mature
            spliced mRNA includes at least one additional non-coding exon.
REFERENCE   7  (bases 1 to 1799)
  AUTHORS   Butcher,N.J., Arulpragasam,A., Pope,C. and Minchin,R.F.
  TITLE     Identification of a minimal promoter sequence for the human
            N-acetyltransferase Type I gene that binds AP-1 (activator protein
            1) and YY-1 (Yin and Yang 1)
  JOURNAL   Biochem J 376 (Pt 2), 441-448 (2003)
   PUBMED   12946272
  REMARK    GeneRIF: Identification of minimal promoter sequences for
            transcription factor binding sites in the human N-acetyltransferase
            Type I gene. that binds
REFERENCE   8  (bases 1 to 1799)
  AUTHORS   Dupret,J.M. and Grant,D.M.
  TITLE     Site-directed mutagenesis of recombinant human arylamine
            N-acetyltransferase expressed in Escherichia coli. Evidence for
            direct involvement of Cys68 in the catalytic mechanism of
            polymorphic human NAT2
  JOURNAL   J Biol Chem 267 (11), 7381-7385 (1992)
   PUBMED   1559981
REFERENCE   9  (bases 1 to 1799)
  AUTHORS   Blum,M., Grant,D.M., McBride,W., Heim,M. and Meyer,U.A.
  TITLE     Human arylamine N-acetyltransferase genes: isolation, chromosomal
            localization, and functional expression
  JOURNAL   DNA Cell Biol 9 (3), 193-203 (1990)
   PUBMED   2340091
REFERENCE   10 (bases 1 to 1799)
  AUTHORS   Ohsako,S. and Deguchi,T.
  TITLE     Cloning and expression of cDNAs for polymorphic and monomorphic
            arylamine N-acetyltransferases from human liver
  JOURNAL   J Biol Chem 265 (8), 4630-4634 (1990)
   PUBMED   1968463
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG655073.1, BC047666.1,
            BX647521.1 and BU753390.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000662.7.
            
            Summary: This gene is one of two arylamine N-acetyltransferase
            (NAT) genes in the human genome, and is orthologous to the mouse
            and rat Nat2 genes. The enzyme encoded by this gene catalyzes the
            transfer of an acetyl group from acetyl-CoA to various arylamine
            and hydrazine substrates. This enzyme helps metabolize drugs and
            other xenobiotics, and functions in folate catabolism. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (5, also known as Type IIA) lacks
            two alternate exons in the 5' UTR, compared to variant 1, and is
            the predominant transcript. This variant is transcribed from a
            promoter known as P1, promoter 2, or NATb promoter. Variants 1-6
            and 9 all encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189667.236840.1,
                                           SRR5189667.205165.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000307719.9/ ENSP00000307218.4
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BG655073.1         34-48
            16-1244             BC047666.1         1-1229
            1245-1248           BX647521.1         1344-1347
            1249-1380           BX647521.1         1349-1480
            1381-1799           BU753390.1         17-435              c
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p22"
     gene            1..1799
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /note="N-acetyltransferase 1"
                     /db_xref="GeneID:9"
                     /db_xref="HGNC:HGNC:7645"
                     /db_xref="MIM:108345"
     exon            1..72
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /inference="alignment:Splign:2.1.0"
     exon            73..151
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /inference="alignment:Splign:2.1.0"
     exon            152..1799
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /inference="alignment:Splign:2.1.0"
     CDS             158..1030
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /EC_number="2.3.1.5"
                     /note="isoform a is encoded by transcript variant 5;
                     N-acetyltransferase type 1; monomorphic arylamine
                     N-acetyltransferase; arylamide acetylase 1;
                     N-acetyltransferase 1 (arylamine N-acetyltransferase)"
                     /codon_start=1
                     /product="arylamine N-acetyltransferase 1 isoform a"
                     /protein_id="NP_000653.3"
                     /db_xref="CCDS:CCDS6007.1"
                     /db_xref="GeneID:9"
                     /db_xref="HGNC:HGNC:7645"
                     /db_xref="MIM:108345"
                     /translation="MDIEAYLERIGYKKSRNKLDLETLTDILQHQIRAVPFENLNIHC
                     GDAMDLGLEAIFDQVVRRNRGGWCLQVNHLLYWALTTIGFETTMLGGYVYSTPAKKYS
                     TGMIHLLLQVTIDGRNYIVDAGFGRSYQMWQPLELISGKDQPQVPCVFRLTEENGFWY
                     LDQIRREQYIPNEEFLHSDLLEDSKYRKIYSFTLKPRTIEDFESMNTYLQTSPSSVFT
                     SKSFCSLQTPDGVHCLVGFTLTHRRFNYKDNTDLIEFKTLSEEEIEKVLKNIFNISLQ
                     RKLVPKHGDRFFTI"
     regulatory      1361..1366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /note="hexamer: AATAAA"
     polyA_site      1380
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /note="major polyA site"
     regulatory      1400..1405
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /note="hexamer: ATTAAA"
     polyA_site      1421
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /note="major polyA site"
     regulatory      1764..1769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /note="hexamer: AATAAA"
     polyA_site      1799
                     /gene="NAT1"
                     /gene_synonym="AAC1; MNAT; NAT-1; NATI"
                     /note="major polyA site"
ORIGIN      
        1 acttcctcat agaccttgga tgtgggagga ttgcattcag tctagttcct ggttgccggc
       61 tgaaataacc tgaattcaag ccaggaagaa gcagcaatct gtcttctgga ttaaaactga
      121 agatcaacct actttcaact tactaagaaa ggggatcatg gacattgaag catatcttga
      181 aagaattggc tataagaagt ctaggaacaa attggacttg gaaacattaa ctgacattct
      241 tcaacaccag atccgagctg ttccctttga gaaccttaac atccattgtg gggatgccat
      301 ggacttaggc ttagaggcca tttttgatca agttgtgaga agaaatcggg gtggatggtg
      361 tctccaggtc aatcatcttc tgtactgggc tctgaccact attggttttg agaccacgat
      421 gttgggaggg tatgtttaca gcactccagc caaaaaatac agcactggca tgattcacct
      481 tctcctgcag gtgaccattg atggcaggaa ctacattgtc gatgctgggt ttggacgctc
      541 ataccagatg tggcagcctc tggagttaat ttctgggaag gatcagcctc aggtgccttg
      601 tgtcttccgt ttgacggaag agaatggatt ctggtatcta gaccaaatca gaagggaaca
      661 gtacattcca aatgaagaat ttcttcattc tgatctccta gaagacagca aataccgaaa
      721 aatctactcc tttactctta agcctcgaac aattgaagat tttgagtcta tgaatacata
      781 cctgcagaca tctccatcat ctgtgtttac tagtaaatca ttttgttcct tgcagacccc
      841 agatggggtt cactgtttgg tgggcttcac cctcacccat aggagattca attataagga
      901 caatacagat ctaatagagt tcaagactct gagtgaggaa gaaatagaaa aagtgctgaa
      961 aaatatattt aatatttcct tgcagagaaa gcttgtgccc aaacatggtg atagattttt
     1021 tactatttag aataaggagt aaaacaatct tgtctatttg tcatccagct caccagttat
     1081 caactgacga cctatcatgt atcttctgta cccttacctt attttgaaga aaatcctaga
     1141 catcaaatca tttcacctat aaaaatgtca tcatatataa ttaaacagct ttttaaagaa
     1201 acataaccac aaaccttttc aaataataat aataataata ataaaaaatg tattttaaag
     1261 atggcctgtg gttatcttgg aaattggtga tttatgctag aaagctttta atgttggttt
     1321 attgttgaat tcctagaaaa gttttattgg tagatgagta aataaaatat tgtaaaaaaa
     1381 cttattgtct ataaagtata ttaaaacatt gttggctaat ataatttgaa aaaaagtggt
     1441 tttttggaag acttaggata ttatggtgct acataatttt tcctcgatgc tctcttcctc
     1501 tcatctttct tgtctcttaa attactttac ttccttgcac actttgccat acaagaatga
     1561 acatgagctt ttcttgtgta gatctgagtt gaaatcctgt ggacactggg cgaattactt
     1621 tttagatctg tagctctgac tcctcaggca taaaatggga ataatgcttt tacagtttag
     1681 tggcggaact aaactcccaa aattatttgt tatatggatc aagtaataac gtcagtaatg
     1741 tttttggtac aaagtcatta tttaataaaa gttattgttc catcttgctt gccccccca
//
