LOCUS       NM_004851               1357 bp    mRNA    linear   PRI 05-MAR-2025
DEFINITION  Homo sapiens napsin A aspartic peptidase (NAPSA), transcript
            variant 1, mRNA.
ACCESSION   NM_004851
VERSION     NM_004851.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Arciuolo,D., Travaglino,A., Raffone,A., Santoro,A., Inzani,F.,
            Piermattei,A., Bui,L., Scaglione,G., D'Alessandris,N., Valente,M.,
            Fulgione,C., Guida,M., Mollo,A., Insabato,L. and Zannoni,G.F.
  TITLE     P504S/alpha-methylacyl-CoA racemase, HNF1beta and napsin A in
            morular metaplasia and clear cell carcinoma of the endometrium: An
            immunohistochemical analysis
  JOURNAL   Pathol Res Pract 236, 153953 (2022)
   PUBMED   35696768
  REMARK    GeneRIF: P504S/alpha-methylacyl-CoA racemase, HNF1beta and napsin A
            in morular metaplasia and clear cell carcinoma of the endometrium:
            An immunohistochemical analysis.
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Gaydarov,N., Martinelli-Klay,C.P. and Lombardi,T.
  TITLE     A study on the immunohistochemical expression of napsin A in oral
            squamous cell carcinomas, intraepithelial neoplasia, and normal
            oral mucosa
  JOURNAL   J Histotechnol 44 (3), 139-143 (2021)
   PUBMED   33876717
  REMARK    GeneRIF: A study on the immunohistochemical expression of napsin A
            in oral squamous cell carcinomas, intraepithelial neoplasia, and
            normal oral mucosa.
REFERENCE   3  (bases 1 to 1357)
  AUTHORS   Li,W. and Cohen,M.B.
  TITLE     TTF-1, napsin A and CDX2 co-expression in metastatic rectal
            adenocarcinoma to the lung
  JOURNAL   Cytopathology 32 (1), 146-148 (2021)
   PUBMED   32633432
REFERENCE   4  (bases 1 to 1357)
  AUTHORS   Mohammed Salama,M.E.
  TITLE     Role of Napsin A and Survivin Immunohistochemical Expression in
            Bronchogenic Adenocarcinoma
  JOURNAL   Asian Pac J Cancer Prev 21 (11), 3345-3348 (2020)
   PUBMED   33247694
  REMARK    GeneRIF: Role of Napsin A and Survivin Immunohistochemical
            Expression in Bronchogenic Adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1357)
  AUTHORS   Ordonez,N.G.
  TITLE     Napsin A expression in lung and kidney neoplasia: a review and
            update
  JOURNAL   Adv Anat Pathol 19 (1), 66-73 (2012)
   PUBMED   22156835
  REMARK    GeneRIF: napsin A is a useful marker that can assist in the
            diagnosis of both lung adenocarcinomas and renal cell
            carcinomas[review]
            Review article
REFERENCE   6  (bases 1 to 1357)
  AUTHORS   Schauer-Vukasinovic,V., Bur,D., Kling,D., Gruninger,F. and
            Giller,T.
  TITLE     Human napsin A: expression, immunochemical detection, and tissue
            localization
  JOURNAL   FEBS Lett 462 (1-2), 135-139 (1999)
   PUBMED   10580106
REFERENCE   7  (bases 1 to 1357)
  AUTHORS   Chuman,Y., Bergman,A., Ueno,T., Saito,S., Sakaguchi,K.,
            Alaiya,A.A., Franzen,B., Bergman,T., Arnott,D., Auer,G.,
            Appella,E., Jornvall,H. and Linder,S.
  TITLE     Napsin A, a member of the aspartic protease family, is abundantly
            expressed in normal lung and kidney tissue and is expressed in lung
            adenocarcinomas
  JOURNAL   FEBS Lett 462 (1-2), 129-134 (1999)
   PUBMED   10580105
REFERENCE   8  (bases 1 to 1357)
  AUTHORS   Tatnell,P.J., Powell,D.J., Hill,J., Smith,T.S., Tew,D.G. and Kay,J.
  TITLE     Napsins: new human aspartic proteinases. Distinction between two
            closely related genes
  JOURNAL   FEBS Lett 441 (1), 43-48 (1998)
   PUBMED   9877162
REFERENCE   9  (bases 1 to 1357)
  AUTHORS   Blundell,T.L., Guruprasad,K., Albert,A., Williams,M., Sibanda,B.L.
            and Dhanaraj,V.
  TITLE     The aspartic proteinases. An historical overview
  JOURNAL   Adv Exp Med Biol 436, 1-13 (1998)
   PUBMED   9561193
REFERENCE   10 (bases 1 to 1357)
  AUTHORS   Koelsch,G., Mares,M., Metcalf,P. and Fusek,M.
  TITLE     Multiple functions of pro-parts of aspartic proteinase zymogens
  JOURNAL   FEBS Lett 343 (1), 6-10 (1994)
   PUBMED   8163018
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF098484.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004851.2.
            
            Summary: This gene encodes a member of the peptidase A1 family of
            aspartic proteases. The encoded preproprotein is proteolytically
            processed to generate an activation peptide and the mature
            protease. The activation peptides of aspartic proteinases function
            as inhibitors of the protease active site. These peptide segments,
            or pro-parts, are deemed important for correct folding, targeting,
            and control of the activation of aspartic proteinase zymogens. The
            encoded protease may play a role in the proteolytic processing of
            pulmonary surfactant protein B in the lung and may function in
            protein catabolism in the renal proximal tubules. This gene has
            been described as a marker for lung adenocarcinoma and renal cell
            carcinoma. [provided by RefSeq, Feb 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF098484.1, AK308013.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144333, SAMEA2147920
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253719.7/ ENSP00000253719.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1357              AF098484.1         36-1392
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..1357
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /note="napsin A aspartic peptidase"
                     /db_xref="GeneID:9476"
                     /db_xref="HGNC:HGNC:13395"
                     /db_xref="MIM:605631"
     exon            1..101
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..1281
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /EC_number="3.4.23.5"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; pronapsin A; napsin-A; ASP4; asp 4; napsin-1;
                     TA01/TA02; aspartyl protease 4; kidney-derived aspartic
                     protease-like protein; NR1H2 antisense RNA 1"
                     /codon_start=1
                     /product="napsin-A isoform 1 preproprotein"
                     /protein_id="NP_004842.1"
                     /db_xref="CCDS:CCDS12794.1"
                     /db_xref="GeneID:9476"
                     /db_xref="HGNC:HGNC:13395"
                     /db_xref="MIM:605631"
                     /translation="MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRILNLL
                     RGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNL
                     WVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGI
                     KGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFS
                     FYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAK
                     GCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVW
                     FNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKS
                     SARVGLARARTRGADLGWGETAQAQFPG"
     exon            102..243
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            244..367
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            368..486
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            487..686
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            687..809
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            810..954
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1053
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1357
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1333..1338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /note="hexamer: AGTAAA"
     polyA_site      1357
                     /gene="NAPSA"
                     /gene_synonym="KAP; Kdap; NAP1; NAPA; NR1H2-AS1; SNAPA"
                     /note="major polyA site"
ORIGIN      
        1 acacccaggt ccccagcgat gtctccacca ccgctgctgc aacccctgct gctgctgctg
       61 cctctgctga atgtggagcc ttccggggcc acactgatcc gcatccctct tcatcgagtc
      121 caacctggac gcaggatcct gaacctactg aggggatgga gagaaccagc agagctcccc
      181 aagttggggg ccccatcccc tggggacaag cccatcttcg tacctctctc gaactacagg
      241 gatgtgcagt attttgggga aattgggctg ggaacgcctc cacaaaactt cactgttgcc
      301 tttgacactg gctcctccaa tctctgggtc ccgtccagga gatgccactt cttcagtgtg
      361 ccctgctggt tacaccaccg atttgatccc aaagcctcta gctccttcca ggccaatggg
      421 accaagtttg ccattcaata tggaactggg cgggtagatg gaatcctgag cgaggacaag
      481 ctgactattg gtggaatcaa gggtgcatca gtgattttcg gggaggctct ctgggagccc
      541 agcctggtct tcgcttttgc ccattttgat gggatattgg gcctcggttt tcccattctg
      601 tctgtggaag gagttcggcc cccgatggat gtactggtgg agcaggggct attggataag
      661 cctgtcttct ccttttacct caacagggac cctgaagagc ctgatggagg agagctggtc
      721 ctggggggct cggacccggc acactacatc ccacccctca ccttcgtgcc agtcacggtc
      781 cctgcctact ggcagatcca catggagcgt gtgaaggtgg gcccagggct gactctctgt
      841 gccaagggct gtgctgccat cctggatacg ggcacgtccc tcatcacagg acccactgag
      901 gagatccggg ccctgcatgc agccattggg ggaatcccct tgctggctgg ggagtacatc
      961 atcctgtgct cggaaatccc aaagctcccc gcagtctcct tccttcttgg gggggtctgg
     1021 tttaacctca cggcccatga ttacgtcatc cagactactc gaaatggcgt ccgcctctgc
     1081 ttgtccggtt tccaggccct ggatgtccct ccgcctgcag ggcccttctg gatcctcggt
     1141 gacgtcttct tggggacgta tgtggccgtc ttcgaccgcg gggacatgaa gagcagcgcc
     1201 cgggtgggcc tggcgcgcgc tcgcactcgc ggagcggacc tcggatgggg agagactgcg
     1261 caggcgcagt tccccgggtg acgcccaagt gaagcgcatg cgcagcgggt ggtcgcggag
     1321 gtcctgctac ccagtaaaaa tccactattt ccattga
//
