LOCUS       NM_001122838            5140 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens N-acyl phosphatidylethanolamine phospholipase D
            (NAPEPLD), transcript variant 1, mRNA.
ACCESSION   NM_001122838
VERSION     NM_001122838.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Igarashi,M., Watanabe,K., Tsuduki,T., Kimura,I. and Kubota,N.
  TITLE     NAPE-PLD controls OEA synthesis and fat absorption by regulating
            lipoprotein synthesis in an in vitro model of intestinal epithelial
            cells
  JOURNAL   FASEB J 33 (3), 3167-3179 (2019)
   PUBMED   30399323
  REMARK    GeneRIF: Transcriptome analysis revealed that deletion of NAPE-PLD
            activates a transcriptional program for nutrient transportation,
            including lipids and lipoproteins, and inactivates cell-cycle or
            mitosis-related genes in Caco-2 cells.
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Si,P., Liu,S., Tong,D., Cheng,M., Wang,L. and Cheng,X.
  TITLE     [Association of polymorphisms of NAPE-PLD and FAAH genes with
            schizophrenia in Chinese Han population]
  JOURNAL   Zhonghua Yi Xue Yi Chuan Xue Za Zhi 35 (2), 215-218 (2018)
   PUBMED   29652995
  REMARK    GeneRIF: The AC genotype and C allele of NAPE-PLD rs12540583 locus
            are risk factors for schizophrenia.
REFERENCE   3  (bases 1 to 5140)
  AUTHORS   Margheritis,E., Castellani,B., Magotti,P., Peruzzi,S., Romeo,E.,
            Natali,F., Mostarda,S., Gioiello,A., Piomelli,D. and Garau,G.
  TITLE     Bile Acid Recognition by NAPE-PLD
  JOURNAL   ACS Chem Biol 11 (10), 2908-2914 (2016)
   PUBMED   27571266
REFERENCE   4  (bases 1 to 5140)
  AUTHORS   Magotti,P., Bauer,I., Igarashi,M., Babagoli,M., Marotta,R.,
            Piomelli,D. and Garau,G.
  TITLE     Structure of human N-acylphosphatidylethanolamine-hydrolyzing
            phospholipase D: regulation of fatty acid ethanolamide biosynthesis
            by bile acids
  JOURNAL   Structure 23 (3), 598-604 (2015)
   PUBMED   25684574
  REMARK    GeneRIF: NAPE-PLD forms homodimers partly separated by an internal
            channel and uniquely adapted to associate with phospholipids.
REFERENCE   5  (bases 1 to 5140)
  AUTHORS   Pisanu,C., Congiu,D., Costa,M., Sestu,M., Chillotti,C., Ardau,R.,
            Deiana,V., Manchia,M., Squassina,A. and Del Zompo,M.
  TITLE     No association of endocannabinoid genes with bipolar disorder or
            lithium response in a Sardinian sample
  JOURNAL   Psychiatry Res 210 (3), 887-890 (2013)
   PUBMED   24126189
  REMARK    GeneRIF: our results do not support a clear role of FAAH, CNR1 and
            NAPE-PLD in BD and lithium response.
REFERENCE   6  (bases 1 to 5140)
  AUTHORS   Petersen,G., Pedersen,A.H., Pickering,D.S., Begtrup,M. and
            Hansen,H.S.
  TITLE     Effect of synthetic and natural phospholipids on
            N-acylphosphatidylethanolamine-hydrolyzing phospholipase D activity
  JOURNAL   Chem Phys Lipids 162 (1-2), 53-61 (2009)
   PUBMED   19715685
  REMARK    GeneRIF: The human NAPE-PLD was activated by
            phosphatidylethanolamine and inhibited by the beta-lactamase
            substrate nitrocefin.
REFERENCE   7  (bases 1 to 5140)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   8  (bases 1 to 5140)
  AUTHORS   Egertova,M., Simon,G.M., Cravatt,B.F. and Elphick,M.R.
  TITLE     Localization of N-acyl phosphatidylethanolamine phospholipase D
            (NAPE-PLD) expression in mouse brain: A new perspective on
            N-acylethanolamines as neural signaling molecules
  JOURNAL   J Comp Neurol 506 (4), 604-615 (2008)
   PUBMED   18067139
REFERENCE   9  (bases 1 to 5140)
  AUTHORS   Curtiss,N.P., Bonifas,J.M., Lauchle,J.O., Balkman,J.D., Kratz,C.P.,
            Emerling,B.M., Green,E.D., Le Beau,M.M. and Shannon,K.M.
  TITLE     Isolation and analysis of candidate myeloid tumor suppressor genes
            from a commonly deleted segment of 7q22
  JOURNAL   Genomics 85 (5), 600-607 (2005)
   PUBMED   15820312
REFERENCE   10 (bases 1 to 5140)
  AUTHORS   Okamoto,Y., Morishita,J., Tsuboi,K., Tonai,T. and Ueda,N.
  TITLE     Molecular characterization of a phospholipase D generating
            anandamide and its congeners
  JOURNAL   J Biol Chem 279 (7), 5298-5305 (2004)
   PUBMED   14634025
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC007683.5.
            
            On Aug 14, 2020 this sequence version replaced NM_001122838.2.
            
            Summary: NAPEPLD is a phospholipase D type enzyme that catalyzes
            the release of N-acylethanolamine (NAE) from
            N-acyl-phosphatidylethanolamine (NAPE) in the second step of the
            biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed
            14634025]).[supplied by OMIM, Oct 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.151255.1, CR936639.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000465647.6/ ENSP00000419188.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-289               AC007683.5         93199-93487
            290-599             AC007683.5         113506-113815
            600-1246            AC007683.5         122075-122721
            1247-1361           AC007683.5         127124-127238
            1362-5140           AC007683.5         138744-142522
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q22.1"
     gene            1..5140
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /note="N-acyl phosphatidylethanolamine phospholipase D"
                     /db_xref="GeneID:222236"
                     /db_xref="HGNC:HGNC:21683"
                     /db_xref="MIM:612334"
     exon            1..289
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            290..599
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     CDS             306..1487
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /EC_number="3.1.4.54"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NAPE-hydrolyzing phospholipase D;
                     N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase
                     D"
                     /codon_start=1
                     /product="N-acyl-phosphatidylethanolamine-hydrolyzing
                     phospholipase D isoform 1"
                     /protein_id="NP_001116310.1"
                     /db_xref="CCDS:CCDS5729.1"
                     /db_xref="GeneID:222236"
                     /db_xref="HGNC:HGNC:21683"
                     /db_xref="MIM:612334"
                     /translation="MDENESNQSLMTSSQYPKEAVRKRQNSARNSGASDSSRFSRKSF
                     KLDYRLEEDVTKSKKGKDGRFVNPWPTWKNPSIPNVLRWLIMEKDHSSVPSSKEELDK
                     ELPVLKPYFITNPEEAGVREAGLRVTWLGHATVMVEMDELIFLTDPIFSSRASPSQYM
                     GPKRFRRSPCTISELPPIDAVLISHNHYDHLDYNSVIALNERFGNELRWFVPLGLLDW
                     MQKCGCENVIELDWWEENCVPGHDKVTFVFTPSQHWCKRTLMDDNKVLWGSWSVLGPW
                     NRFFFAGDTGYCPAFEEIGKRFGPFDLAAIPIGAYEPRWFMKYQHVDPEEAVRIHTDV
                     QTKKSMAIHWGTFALANEHYLEPPVKLNEALERYGLNAEDFFVLKHGESRYLNNDDEN
                     F"
     exon            600..1246
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1361
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..5140
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5114..5119
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /note="hexamer: AATAAA"
     polyA_site      5140
                     /gene="NAPEPLD"
                     /gene_synonym="C7orf18; FMP30; NAPE-PLD"
                     /note="major polyA site"
ORIGIN      
        1 acactgctga gcctggggtg gtttggaatt cttcggcgaa gtggagcggg ctgagagtgg
       61 gtttcccgtc cccgagccct ccctcatctc ggagatgccg cacttgtgag cgacagggct
      121 tcatttgaat tttcgcacct cgcgagttgc ggagcctgca tctctgaagc tgcctcggtg
      181 aagagccaca gcattatttt ttttctcttt gggagagtag ctgtctggga tttgagatcc
      241 tatcttccct gcaaattacc aggttgcatc ttcactgata ggaatacatt tcttcaccaa
      301 aggacatgga tgaaaatgaa agcaaccagt ctctgatgac aagcagccaa tatcctaaag
      361 aagcagtaag aaaacgtcaa aattcagcac ggaattccgg agcaagtgat tcttctaggt
      421 tttctaggaa aagcttcaaa ctggattata gactagaaga agatgtaact aaatccaaga
      481 aaggaaaaga tgggagattt gtgaatccgt ggccaacatg gaaaaacccc tctattccaa
      541 atgttctcag atggctgata atggagaaag atcacagcag tgttccaagt tctaaagagg
      601 aactagacaa agaactccca gtgcttaagc catattttat cactaaccct gaagaagctg
      661 gagtgaggga agctggctta agagtcacat ggctgggaca tgccacggta atggtggaaa
      721 tggatgagct catatttctc acggatccca tctttagctc tcgtgcttca ccatcgcagt
      781 acatgggtcc aaagcgattt cgtcgttccc cgtgcacaat aagtgaactc cctccaatag
      841 atgcggtcct tatcagtcac aaccactatg accatctgga ctacaattct gtcattgctt
      901 tgaatgagcg atttggtaat gagttgagat ggtttgtgcc tttgggtctc cttgactgga
      961 tgcaaaaatg tggctgtgag aatgtgattg agttggactg gtgggaggag aattgtgtcc
     1021 ccggacatga taaggtcact tttgtcttta caccttccca gcactggtgt aaaaggactc
     1081 taatggatga caacaaggtg ctatggggca gctggtctgt cttggggcct tggaatcgat
     1141 tttttttcgc aggagatact ggttattgcc ctgcttttga agagatagga aaaagatttg
     1201 gaccttttga ccttgcagct attcccatcg gagcttatga accgaggtgg tttatgaaat
     1261 accagcatgt agacccagaa gaagctgtaa ggattcacac tgatgtccaa acaaagaaat
     1321 ctatggcaat tcactgggga acttttgcct tagcaaatga gcattactta gagcctccag
     1381 tgaagctgaa tgaagctcta gagagatacg gacttaacgc tgaagatttt tttgtcttga
     1441 agcatggaga atcaagatac ctaaataatg atgatgaaaa cttttaataa atgtgagcac
     1501 aggcactttt aatgaaaaag gcatcatcta agtttaaaaa caaatgaaaa gacttagaaa
     1561 ttcatgaata ttgtgattat tttacatttg gaaacaactt ctgcaagttt atgttttatg
     1621 ttttgtgtaa taacttgctt cctagtatga tcagctcatg tacactatga gagggttcag
     1681 gggtgggtca tttaaataat gaattggcta atgtggattt taaatttcat atcatgatat
     1741 aagcactgca atggaaatgt ttttactaaa acattatatt tttgtggtac tagaatttcc
     1801 tgaggtgaaa aaccgatatt gaagattcat ttttcttttt tccattcttc aggaccttcg
     1861 gcaaggtgag tcaaggctct ctacctacct agatgttttt cagaggccta caaatacaag
     1921 tatctttctt atatgcacat tctaataaat gatgaaattc agtccaacat gcttattata
     1981 aacatagcaa ttcttgctct atcaaattag cttatatgcc aaatattaaa tatggtttat
     2041 ttaggtaagc ataaaaacat aggggctatc atttttaaag acttgatttt ctctattgtg
     2101 gtaaaatata taaatacata tttgccattt taaacattta ccattttaac catttttaag
     2161 tgtacagttc tttcgcatta atgcacccat gaccaccatt cacttaaatt tttaaaagaa
     2221 cagtatattc tttttttcct agagccttca gatgagaggg aacagtatat tctattgcac
     2281 gtctacaatt cccaaaagac ctcttacttt gggaggccaa ggtgggtggg tcacttgaac
     2341 ccaggagttt gacatcaacc tgggcaacat ggtgacctcc cgtctctctc ggaaaaaaaa
     2401 aaagaaaaaa gaaaaagccg ggtgaggtgg tgtgtacctg tagtcacagg tacttgggag
     2461 gctgaggtgg gaggatcact tgagcccagg agatagaggc tgcagtgagc tgtgatcgta
     2521 ccactgcact ccagcctggg cgacaaaatg agaccctgtc tcaaaagaaa gccctctcct
     2581 tagctgagca gaggaaggga aggagtgtgg ctatgagaat atgatttatg ccattttctg
     2641 tttttaaatc tagaagatct tctaagcaca aatacagcta caatgaaata ttttacagac
     2701 aaaatgttaa tagaccatat tctttgaatt aaatttgttt ttaattttct ctacacattt
     2761 tttttttcct ggagtctctt agctctaaat atatcaatca gatttataat tttttttacc
     2821 tgattcagat gtcttacatt tttatattaa atgaacctta agcatgattc ttttggtaag
     2881 ccagtatgaa tgccagtggt tggggggcgg ggggggagtc agttgacata agatttagtc
     2941 ctaataagga ctctgtattc acttgattat tctgaccctt cctaagggag ggaggtggat
     3001 tagataccac tggaggccca ttctgtattc ctaatccagt ctcagcactt tattcataca
     3061 aaataatcaa aataggtttt ctacaccaaa tgctacccag gcagtgctct ataatttatt
     3121 tgcacacctg tatgtgtggt gccacgttag ccactaaatc tgaactttta ccctgctttc
     3181 atcatggatt tttttgtgta acctgaaaca agtcaccagt gctcactgtg cctgtttcct
     3241 catttgcaac aagaggataa ttccgaccta cctctgaaaa atgtgtagat taaatgagtt
     3301 tttaaagcac tttagaaaat ataagggaga aaatgatttc ttaagatatc actgcctaat
     3361 atagacaggt atatttgaaa gctaaataca ggttaatgct tttgataatg tattgttcct
     3421 gttgcaaatt taagcattat atattaggag actaaacatt gaaaactttc actgattttt
     3481 aaattggagt aatagcctaa ataagtgtaa ataatcttta tcaaaattta gtgtttatct
     3541 acaaagatgc gaatcagtca aggggaaatg tgtatgcaat gtgtgtgtat atgatgtatg
     3601 cgaacatatg tgattttggg gtactaaagc acaaaaaaat cccccataaa ataaaccttc
     3661 ctgaaaagac tgatcataaa ctttttcact ccaagcagat gtgaatcaag atgttaggtt
     3721 tatggtgagt tagctttgag gtgtgttgcc ctaatactag ccagcgttgt tgtagccata
     3781 ttccctgctg ctgcacgccg aattgaaaat ccatctctta cctcttctct atgaagcaag
     3841 ccaggtatgt cttgcttaag tatttcttct ccatgaatgg ccttaacatt attttgtatt
     3901 cacgtgtact gtttaactga gggtctcaaa gcactttaaa ctgttgtgtt ctatttccac
     3961 tcgtctggcc tgcctcattt ttctaggccc ttttgtaagc tgtaattttt tattaccact
     4021 catgttcttt ctacactgac actattcatt aagaacatca agcttcatct ctgcttacag
     4081 aatctcacct tattgagtgg gttccacttt atttttcatt tgttatctac attttgaagg
     4141 gtttatgtgc aaagtgaaaa caaattttga aaaataagac aaatccattc tcttcttcaa
     4201 ttcagtctaa tatgcattgt agtgtctttt aatcagtctt atcctctaag tcaacttcat
     4261 tttcctcttc gtgaataccc atagcaatat gtaagatttt attgaagttt cctgtgataa
     4321 ctgaggaata ctttgctatg cctcactgcc caatagggtg ggtcatcaat taggagaaat
     4381 cccaaattat aaaagccaac atatgccgtg acctacgtag gaaaagatgt atttgtgcat
     4441 cttctttctg ggtagttaaa aattactccc tcttttctct agcattttga catttcagac
     4501 tcaatgctct gatctctagg gagtgtgatt attcaaacta atgagaggac aagaagacac
     4561 agttttagaa tctactgctg ctaaagcaca ttaaaaacaa atcctggttt ttaatgtatg
     4621 catagtttca tatttaatga atatgctttc attaaatagg gaggtagatt tgaaaaaacc
     4681 gttataaaga aatgtgcctt ttggggaatg aatacttttg ttcatgtcta gcccactggg
     4741 aattttgttt tcaccttata ctgagtcctt tttctaatgt aaaaatattt cttccaagtt
     4801 tgatttctgt atcagggtaa cattgagaac acacatattt ctgtttgagg atttgtgact
     4861 tcctatggaa acagtcttgc atctatgtat ttacaaatga aatttaaagt attcttaaag
     4921 ctttgcctta aatactgctt gcatactgat ttacttaatg ctaagcatgg caggggttat
     4981 aaatttaaat actataagac ctggctgaat cccagatgta tcaataaatt gttatagtag
     5041 cttatggaaa atgctattaa agataaattt tcatacattg tatttttagt caagtaaaat
     5101 atggatgaaa attaataaag tactgtcaag ggaaaaaata
//
