LOCUS       NM_003827               1662 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NSF attachment protein alpha (NAPA), transcript
            variant 1, mRNA.
ACCESSION   NM_003827
VERSION     NM_003827.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Ramanagoudr-Bhojappa,R., Miao,Y. and Vig,M.
  TITLE     High affinity associations with alpha-SNAP enable calcium entry via
            Orai1 channels
  JOURNAL   PLoS One 16 (10), e0258670 (2021)
   PUBMED   34653219
  REMARK    GeneRIF: High affinity associations with alpha-SNAP enable calcium
            entry via Orai1 channels.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1662)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 1662)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 1662)
  AUTHORS   Hubel,P., Urban,C., Bergant,V., Schneider,W.M., Knauer,B.,
            Stukalov,A., Scaturro,P., Mann,A., Brunotte,L., Hoffmann,H.H.,
            Schoggins,J.W., Schwemmle,M., Mann,M., Rice,C.M. and Pichlmair,A.
  TITLE     A protein-interaction network of interferon-stimulated genes
            extends the innate immune system landscape
  JOURNAL   Nat Immunol 20 (4), 493-502 (2019)
   PUBMED   30833792
REFERENCE   6  (bases 1 to 1662)
  AUTHORS   Subramaniam,V.N., Loh,E. and Hong,W.
  TITLE     N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF
            attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5
            Golgi SNAP receptors (SNARE) complex
  JOURNAL   J Biol Chem 272 (41), 25441-25444 (1997)
   PUBMED   9325254
REFERENCE   7  (bases 1 to 1662)
  AUTHORS   Lemons,P.P., Chen,D., Bernstein,A.M., Bennett,M.K. and
            Whiteheart,S.W.
  TITLE     Regulated secretion in platelets: identification of elements of the
            platelet exocytosis machinery
  JOURNAL   Blood 90 (4), 1490-1500 (1997)
   PUBMED   9269766
REFERENCE   8  (bases 1 to 1662)
  AUTHORS   Whiteheart,S.W., Griff,I.C., Brunner,M., Clary,D.O., Mayer,T.,
            Buhrow,S.A. and Rothman,J.E.
  TITLE     SNAP family of NSF attachment proteins includes a brain-specific
            isoform
  JOURNAL   Nature 362 (6418), 353-355 (1993)
   PUBMED   8455721
REFERENCE   9  (bases 1 to 1662)
  AUTHORS   Whiteheart,S.W., Brunner,M., Wilson,D.W., Wiedmann,M. and
            Rothman,J.E.
  TITLE     Soluble N-ethylmaleimide-sensitive fusion attachment proteins
            (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes
  JOURNAL   J Biol Chem 267 (17), 12239-12243 (1992)
   PUBMED   1601890
REFERENCE   10 (bases 1 to 1662)
  AUTHORS   Wilson,D.W., Whiteheart,S.W., Wiedmann,M., Brunner,M. and
            Rothman,J.E.
  TITLE     A multisubunit particle implicated in membrane fusion
  JOURNAL   J Cell Biol 117 (3), 531-538 (1992)
   PUBMED   1315316
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC028234.1, AK126519.1 and
            AA542963.1.
            
            On Nov 24, 2018 this sequence version replaced NM_003827.3.
            
            Summary: This gene encodes a member of the soluble NSF attachment
            protein (SNAP) family. SNAP proteins play a critical role in the
            docking and fusion of vesicles to target membranes as part of the
            20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the
            completion of membrane fusion by mediating the interaction of
            N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated
            and membrane-associated SNAP receptor (SNARE) complex, and
            stimulating the ATPase activity of NSF. Alternatively spliced
            transcript variants have been observed for this gene. [provided by
            RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028234.1, SRR3476690.684504.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263354.8/ ENSP00000263354.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1114              BC028234.1         14-1127
            1115-1633           AK126519.1         3352-3870
            1634-1662           AA542963.1         9-37                c
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.32-q13.33"
     gene            1..1662
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /note="NSF attachment protein alpha"
                     /db_xref="GeneID:8775"
                     /db_xref="HGNC:HGNC:7641"
                     /db_xref="MIM:603215"
     exon            1..221
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     CDS             124..1011
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /note="alpha-SNAP; N-ethylmaleimide-sensitive factor
                     attachment protein, alpha"
                     /codon_start=1
                     /product="alpha-soluble NSF attachment protein"
                     /protein_id="NP_003818.2"
                     /db_xref="CCDS:CCDS12702.1"
                     /db_xref="GeneID:8775"
                     /db_xref="HGNC:HGNC:7641"
                     /db_xref="MIM:603215"
                     /translation="MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEI
                     YARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAIN
                     CLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSS
                     ANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDM
                     LNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWL
                     TTMLLRIKKTIQGDEEDLR"
     exon            222..301
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            302..418
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            419..465
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            466..543
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            544..599
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            600..684
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            685..789
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            790..858
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            859..909
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1662
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1638..1643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /note="hexamer: AATAAA"
     polyA_site      1662
                     /gene="NAPA"
                     /gene_synonym="SNAPA"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgccggt gcgacgtcaa cgcagccggg cgagttttac cgatctgtgt tccgcggccc
       61 ggccgcggct gagtcttccc agggtcaggg tcaggcgctt tgctgagtcc ctttgtggcc
      121 gccatggaca attccgggaa ggaagcggag gcgatggcgc tgttggccga ggcggagcgc
      181 aaagtgaaga actcgcagtc cttcttctct ggcctctttg gaggctcatc caaaatagag
      241 gaagcatgcg aaatctacgc cagagcagca aacatgttca aaatggccaa aaactggagt
      301 gctgctggaa acgcgttctg ccaggctgca cagctgcacc tgcagctcca gagcaagcac
      361 gacgcagcca cctgctttgt ggacgctggc aacgcattca agaaagccga cccccaagag
      421 gccattaact gtttgatgcg agcaatcgag atctacacag acatgggccg attcacgatt
      481 gcggccaagc accacatctc cattgctgag atctatgaga cagagttggt ggacatcgag
      541 aaggccattg cccactacga gcagtctgca gactactaca aaggcgagga gtccaacagc
      601 tcagccaaca agtgtctgct gaaggtggct ggttacgctg cgctgctgga gcagtatcag
      661 aaggccattg acatctacga acaggtgggg accaatgcca tggacagccc cctcctcaag
      721 tacagcgcca aagactactt cttcaaggcg gccctctgcc acttctgcat cgacatgctc
      781 aacgccaagc tggctgtcca aaagtatgag gagctgttcc cagctttctc tgattcccgg
      841 gaatgcaagt tgatgaaaaa attgctagag gcccacgagg agcagaatgt ggacagctac
      901 accgagtcgg tgaaggaata cgactccatc tcccggctgg accagtggct caccaccatg
      961 ctgctgcgca tcaagaagac catccagggc gatgaggagg acctgcgcta agccccaccc
     1021 agccccccag tgcccgtctt cctgtcccat ctgctcagag agaggtgggg ccgagacttg
     1081 ctggagagct tccctccttt cccatctggg gagtgccgcg ggccacagtg ggcaggtggc
     1141 accgggggtc agcatgcagg ggcgccagag gcccaggctg ctggccggac agtcaccctc
     1201 tgttctcgct acatcccttg ccccctgtcc atttatttaa gcccccatag gtgcccttca
     1261 cccccaaaac cagctgtaca gaatctttga tacagaccta tttgctaggg gtgctgccgg
     1321 ggatttgggg tcagcatctg gccccctatc tcctgaccag ctgagtcatg aggccggttt
     1381 ctctctctct cccacttttg tcccccagcc aagctctaaa gcacatgtag ccgctgagac
     1441 ctgctgtttc tgctgggggc aggctcctct tcccccagcc ccgggagcct cccccagctt
     1501 cctgcagccc cgacctctca ggttagaccc tgggccctgg agcttagggg attctcccca
     1561 ccccagcccc acacctgctc cttccctaat gctttgaggt tttcttggtt ggaagctgca
     1621 gctggcccaa gaaagaaaat aaaaaacaac acttttgcat ga
//
