LOCUS       NM_001355281            1886 bp    mRNA    linear   PRI 04-AUG-2024
DEFINITION  Homo sapiens Nanog homeobox retrogene P8 (NANOGP8), mRNA.
ACCESSION   NM_001355281
VERSION     NM_001355281.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Thakur,H. and Mattoo,A.R.
  TITLE     A Restriction Endonuclease-Based Assay to Distinguish NANOGP8
            Retrogene from Parental NANOG
  JOURNAL   Methods Mol Biol 2324, 255-262 (2021)
   PUBMED   34165720
  REMARK    GeneRIF: A Restriction Endonuclease-Based Assay to Distinguish
            NANOGP8 Retrogene from Parental NANOG.
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Ma,X., Wang,B., Wang,X., Luo,Y. and Fan,W.
  TITLE     NANOGP8 is the key regulator of stemness, EMT, Wnt pathway,
            chemoresistance, and other malignant phenotypes in gastric cancer
            cells
  JOURNAL   PLoS One 13 (4), e0192436 (2018)
   PUBMED   29689047
  REMARK    GeneRIF: NANOGP8 is the main regulator of gastric cancer stem
            cells. NANOGP8 is correlated with cell proliferation, migration,
            invasion, clonogenic capacity, beta-catenin accumulation in
            nucleus, and chemoresistance in gastric cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1886)
  AUTHORS   Wang,R., Bhattacharya,R., Ye,X., Fan,F., Boulbes,D.R., Xia,L. and
            Ellis,L.M.
  TITLE     Endothelial cells activate the cancer stem cell-associated NANOGP8
            pathway in colorectal cancer cells in a paracrine fashion
  JOURNAL   Mol Oncol 11 (8), 1023-1034 (2017)
   PUBMED   28453235
  REMARK    GeneRIF: studies demonstrated a paracrine role for endothelial
            cells in regulating the cancer stem cells phenotype and
            chemoresistance in colorectal cancer cells by AKT-mediated
            induction of NANOGP8.
REFERENCE   4  (bases 1 to 1886)
  AUTHORS   Wang,B., Chen,Q., Cao,Y., Ma,X., Yin,C., Jia,Y., Zang,A. and Fan,W.
  TITLE     LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness
            and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1
  JOURNAL   PLoS One 11 (12), e0168904 (2016)
   PUBMED   28033430
  REMARK    GeneRIF: LGR5-expressing fraction of CD54+ cells represents gastric
            cancer CSCs, in which LGR5 is closely associated with stemness and
            EMT core genes
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1886)
  AUTHORS   Kawamura,N., Nimura,K., Nagano,H., Yamaguchi,S., Nonomura,N. and
            Kaneda,Y.
  TITLE     CRISPR/Cas9-mediated gene knockout of NANOG and NANOGP8 decreases
            the malignant potential of prostate cancer cells
  JOURNAL   Oncotarget 6 (26), 22361-22374 (2015)
   PUBMED   26087476
  REMARK    GeneRIF: Results indicate that NANOG and NANOGP8 proteins are
            expressed in prostate cancer cell lines, and NANOG and NANOGP8
            equally contribute to the high malignant potential of prostate
            cancer.
REFERENCE   6  (bases 1 to 1886)
  AUTHORS   Zbinden,M., Duquet,A., Lorente-Trigos,A., Ngwabyt,S.N., Borges,I.
            and Ruiz i Altaba,A.
  TITLE     NANOG regulates glioma stem cells and is essential in vivo acting
            in a cross-functional network with GLI1 and p53
  JOURNAL   EMBO J 29 (15), 2659-2674 (2010)
   PUBMED   20581802
  REMARK    GeneRIF: NANOGP8 is expressed in primary human glioblastoma and is
            required for glioblastoma growth in xenograft assay in mice
REFERENCE   7  (bases 1 to 1886)
  AUTHORS   Jeter,C.R., Badeaux,M., Choy,G., Chandra,D., Patrawala,L., Liu,C.,
            Calhoun-Davis,T., Zaehres,H., Daley,G.Q. and Tang,D.G.
  TITLE     Functional evidence that the self-renewal gene NANOG regulates
            human tumor development
  JOURNAL   Stem Cells 27 (5), 993-1005 (2009)
   PUBMED   19415763
REFERENCE   8  (bases 1 to 1886)
  AUTHORS   Zhang,J., Wang,X., Li,M., Han,J., Chen,B., Wang,B. and Dai,J.
  TITLE     NANOGP8 is a retrogene expressed in cancers
  JOURNAL   FEBS J 273 (8), 1723-1730 (2006)
   PUBMED   16623708
REFERENCE   9  (bases 1 to 1886)
  AUTHORS   Booth,H.A. and Holland,P.W.
  TITLE     Eleven daughters of NANOG
  JOURNAL   Genomics 84 (2), 229-238 (2004)
   PUBMED   15233988
REFERENCE   10 (bases 1 to 1886)
  AUTHORS   Hart,A.H., Hartley,L., Ibrahim,M. and Robb,L.
  TITLE     Identification, cloning and expression analysis of the pluripotency
            promoting Nanog genes in mouse and human
  JOURNAL   Dev Dyn 230 (1), 187-198 (2004)
   PUBMED   15108323
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC021231.8.
            
            On Dec 2, 2019 this sequence version replaced NM_001355281.1.
            
            Summary: This gene represents a transcribed retrogene of the Nanog
            homeobox gene. The putative encoded protein may participate in
            reprogramming of cancer cells. In vitro studies using a recombinant
            protein have shown that the protein localizes to the nucleus and
            can promote cell proliferation, similar to the Nanog protein.
            [provided by RefSeq, Sep 2017].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC069807.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000528386.4/ ENSP00000487073.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1886              AC021231.8         84715-86600         c
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q14"
     gene            1..1886
                     /gene="NANOGP8"
                     /gene_synonym="NANOGP1; PN8"
                     /note="Nanog homeobox retrogene P8"
                     /db_xref="GeneID:388112"
                     /db_xref="HGNC:HGNC:23106"
     exon            1..1886
                     /gene="NANOGP8"
                     /gene_synonym="NANOGP1; PN8"
                     /inference="alignment:Splign:2.1.0"
     CDS             186..1103
                     /gene="NANOGP8"
                     /gene_synonym="NANOGP1; PN8"
                     /note="putative homeobox protein NANOGP8; nanog-like
                     protein"
                     /codon_start=1
                     /product="homeobox protein NANOGP8"
                     /protein_id="NP_001342210.1"
                     /db_xref="CCDS:CCDS86444.1"
                     /db_xref="GeneID:388112"
                     /db_xref="HGNC:HGNC:23106"
                     /translation="MSVDPACPQSLPCFEESDCKESSPMPVICGPEENYPSLQMSSAE
                     MPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAENSVAKKEDKVPVKKQKTRTVF
                     SSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPK
                     NSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSN
                     HSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMHFQPNSPASDLEAALEAAGEGLNV
                     IQQTTRYFSTPQTMDLFLNYSMNMQPEDV"
ORIGIN      
        1 gttctgctgg actgagctgg ttgcctcatg ttattatgca ggcaactcac tttatcccaa
       61 tttcttgata cttttccttc tggaggtcct atttctctaa catcttccag aaaagtctta
      121 aagctgcctt aacctttttt ccagtccacc tcttaaattt tttcctcctc ttcctctata
      181 ctaacatgag tgtggatcca gcttgtcccc aaagcttgcc ttgctttgaa gaatccgact
      241 gtaaagaatc ttcacctatg cctgtgattt gtgggcctga agaaaactat ccatccttgc
      301 aaatgtcttc tgctgagatg cctcacacag agactgtctc tcctcttcct tcctccatgg
      361 atctgcttat tcaggacagc cctgattctt ccaccagtcc caaaggcaaa caacccactt
      421 ctgcagagaa tagtgtcgca aaaaaggaag acaaggtccc ggtcaagaaa cagaagacca
      481 gaactgtgtt ctcttccacc cagctgtgtg tactcaatga tagatttcag agacagaaat
      541 acctcagcct ccagcagatg caagaactct ccaacatcct gaacctcagc tacaaacagg
      601 tgaagacctg gttccagaac cagagaatga aatctaagag gtggcagaaa aacaactggc
      661 cgaagaatag caatggtgtg acgcagaagg cctcagcacc tacctacccc agcctctact
      721 cttcctacca ccagggatgc ctggtgaacc cgactgggaa ccttccaatg tggagcaacc
      781 agacctggaa caattcaacc tggagcaacc agacccagaa catccagtcc tggagcaacc
      841 actcctggaa cactcagacc tggtgcaccc aatcctggaa caatcaggcc tggaacagtc
      901 ccttctataa ctgtggagag gaatctctgc agtcctgcat gcacttccag ccaaattctc
      961 ctgccagtga cttggaggct gccttggaag ctgctgggga aggccttaat gtaatacagc
     1021 agaccactag gtattttagt actccacaaa ccatggattt attcctaaac tactccatga
     1081 acatgcaacc tgaagacgtg tgaagatgag tgaaactgat attactcaat ttcagtctgg
     1141 acactggctg aatccttcct ctcccctcct cccatccttc ataggatttt tcttgtttgg
     1201 aaaccacgtg ttctggtttc catgatgccc atccagtcaa tctcatggag ggtggagtat
     1261 ggttggagcc taatcagcga ggtttctttt tttttttttt tttcctattg gatcttcctg
     1321 gagaaaatac tttttttttt tttttttttt gagacggagt cttgctctgt cgcccaggct
     1381 ggagtgcagt ggcgcggtct tggctcactg caagctccgt ctgccgggtt cacgccattc
     1441 tcctgcctca gcctcccgag cagctgggac tacaggcgcc cgccacctcg cccggctaat
     1501 attttgtatt tttagtagag acggggtttc actgtgttag ccaggatggt ctcgatctcc
     1561 tgaccttgtg atccgcccgc ctcggcctcc ctaacagctg ggatttacag gcgtgagcca
     1621 ccgcgccctg cctagaaaag acattttaat aaccttggct gccgtctctg gctatagata
     1681 agtagatcta atacgagttt ggatatcttt agggtttaga atctaacctc aagaataaga
     1741 aatacaagta caaattggtg atgaagatgt attcgtattg tttgggattg ggaggctttg
     1801 cttatttttt aaaaactatt gaggtaaagg gttaagctgt aacatactta attgatttct
     1861 taccgttttt ggctctgttt tgctat
//
