LOCUS       NM_024865               5182 bp    mRNA    linear   PRI 09-OCT-2024
DEFINITION  Homo sapiens Nanog homeobox (NANOG), transcript variant 1, mRNA.
ACCESSION   NM_024865
VERSION     NM_024865.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Kainulainen,K., Niskanen,E.A., Kinnunen,J., Maki-Mantila,K.,
            Hartikainen,K., Paakinaho,V., Malinen,M., Ketola,K. and
            Pasonen-Seppanen,S.
  TITLE     Secreted factors from M1 macrophages drive prostate cancer stem
            cell plasticity by upregulating NANOG, SOX2, and CD44 through
            NFkappaB-signaling
  JOURNAL   Oncoimmunology 13 (1), 2393442 (2024)
   PUBMED   39175947
  REMARK    GeneRIF: Secreted factors from M1 macrophages drive prostate cancer
            stem cell plasticity by upregulating NANOG, SOX2, and CD44 through
            NFkappaB-signaling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Sun,C., Zheng,S., Li,M., Yang,M., Qin,M., Xu,Y., Liang,W., Hu,J.,
            Wang,L., Li,F., Zhou,H. and Yang,L.
  TITLE     [High expression of the stemness-associated molecule Nanog in
            esophageal squamous cell carcinoma tissues promotes tumor invasion
            and metastasis by activating the TGF-beta signaling pathway]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 44 (6), 1209-1216 (2024)
   PUBMED   38977352
  REMARK    GeneRIF: [High expression of the stemness-associated molecule Nanog
            in esophageal squamous cell carcinoma tissues promotes tumor
            invasion and metastasis by activating the TGF-beta signaling
            pathway].
REFERENCE   3  (bases 1 to 5182)
  AUTHORS   Ma,J., Liu,M., Chen,Z., Liu,S., Yang,H. and Duan,M.
  TITLE     NANOG regulate the JAK/STAT3 pathway to promote trophoblast cell
            migration and epithelial-mesenchymal transition (EMT) in
            hypertensive disorders of pregnancy (HDP) through protein
            interaction with CDK1
  JOURNAL   Am J Reprod Immunol 91 (5), e13863 (2024)
   PUBMED   38796740
  REMARK    GeneRIF: NANOG regulate the JAK/STAT3 pathway to promote
            trophoblast cell migration and epithelial-mesenchymal transition
            (EMT) in hypertensive disorders of pregnancy (HDP) through protein
            interaction with CDK1.
REFERENCE   4  (bases 1 to 5182)
  AUTHORS   Ibrahim,H.M., Abdelrahman,A.E., Elsebai,E., Gharieb,S.A.,
            Fahmy,M.M., Ramadan,M.S.H., Wasfy,M.A. and Abdullatif,A.
  TITLE     Clinicopathologic Impact of NANOG, ZEB1, and EpCAM Biomarkers on
            Prognosis of Serous Ovarian Carcinoma
  JOURNAL   Asian Pac J Cancer Prev 24 (9), 3247-3259 (2023)
   PUBMED   37774079
  REMARK    GeneRIF: Clinicopathologic Impact of NANOG, ZEB1, and EpCAM
            Biomarkers on Prognosis of Serous Ovarian Carcinoma.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5182)
  AUTHORS   Knipper,K., Damanakis,A.I., Lyu,S.I., Simon,A.G., Wahler,I.,
            Bruns,C.J., Schroder,W., Schmidt,T. and Quaas,A.
  TITLE     High NANOG expression correlates with worse patients' survival in
            esophageal adenocarcinoma
  JOURNAL   BMC Cancer 23 (1), 669 (2023)
   PUBMED   37461005
  REMARK    GeneRIF: High NANOG expression correlates with worse patients'
            survival in esophageal adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5182)
  AUTHORS   Clark,A.T., Rodriguez,R.T., Bodnar,M.S., Abeyta,M.J., Cedars,M.I.,
            Turek,P.J., Firpo,M.T. and Reijo Pera,R.A.
  TITLE     Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent
            cells and map to chromosome 12p13, a hotspot for teratocarcinoma
  JOURNAL   Stem Cells 22 (2), 169-179 (2004)
   PUBMED   14990856
REFERENCE   7  (bases 1 to 5182)
  AUTHORS   Chambers,I., Colby,D., Robertson,M., Nichols,J., Lee,S., Tweedie,S.
            and Smith,A.
  TITLE     Functional expression cloning of Nanog, a pluripotency sustaining
            factor in embryonic stem cells
  JOURNAL   Cell 113 (5), 643-655 (2003)
   PUBMED   12787505
REFERENCE   8  (bases 1 to 5182)
  AUTHORS   Mitsui,K., Tokuzawa,Y., Itoh,H., Segawa,K., Murakami,M.,
            Takahashi,K., Maruyama,M., Maeda,M. and Yamanaka,S.
  TITLE     The homeoprotein Nanog is required for maintenance of pluripotency
            in mouse epiblast and ES cells
  JOURNAL   Cell 113 (5), 631-642 (2003)
   PUBMED   12787504
REFERENCE   9  (bases 1 to 5182)
  AUTHORS   Cavaleri,F. and Scholer,H.R.
  TITLE     Nanog: a new recruit to the embryonic stem cell orchestra
  JOURNAL   Cell 113 (5), 551-552 (2003)
   PUBMED   12787492
  REMARK    Review article
REFERENCE   10 (bases 1 to 5182)
  AUTHORS   Constantinescu,S.
  TITLE     Stemness, fusion and renewal of hematopoietic and embryonic stem
            cells
  JOURNAL   J Cell Mol Med 7 (2), 103-112 (2003)
   PUBMED   12927049
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC425336.1, AK290896.1,
            AC006517.46 and AI656990.1.
            
            On Nov 22, 2018 this sequence version replaced NM_024865.3.
            
            Summary: The protein encoded by this gene is a DNA binding homeobox
            transcription factor involved in embryonic stem (ES) cell
            proliferation, renewal, and pluripotency. The encoded protein can
            block ES cell differentiation and can also autorepress its own
            expression in differentiating cells. Two transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB093576.1, AK022643.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142363
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000229307.9/ ENSP00000229307.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-242               DC425336.1         8-249
            243-1287            AK290896.1         215-1259
            1288-2070           AC006517.46        69693-70475         c
            2071-2097           AI656990.1         1-27                c
            2098-5182           AC006517.46        66581-69665         c
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..5182
                     /gene="NANOG"
                     /note="Nanog homeobox"
                     /db_xref="GeneID:79923"
                     /db_xref="HGNC:HGNC:20857"
                     /db_xref="MIM:607937"
     exon            1..364
                     /gene="NANOG"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..1131
                     /gene="NANOG"
                     /note="isoform 1 is encoded by transcript variant 1;
                     homeobox protein NANOG; homeobox transcription factor
                     Nanog; homeobox transcription factor Nanog-delta 48"
                     /codon_start=1
                     /product="homeobox protein NANOG isoform 1"
                     /protein_id="NP_079141.2"
                     /db_xref="CCDS:CCDS31736.1"
                     /db_xref="GeneID:79923"
                     /db_xref="HGNC:HGNC:20857"
                     /db_xref="MIM:607937"
                     /translation="MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAE
                     MPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVF
                     SSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPK
                     NSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSN
                     HSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNV
                     IQQTTRYFSTPQTMDLFLNYSMNMQPEDV"
     exon            365..627
                     /gene="NANOG"
                     /inference="alignment:Splign:2.1.0"
     exon            628..714
                     /gene="NANOG"
                     /inference="alignment:Splign:2.1.0"
     exon            715..5182
                     /gene="NANOG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2392..2397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NANOG"
                     /note="hexamer: AATAAA"
     polyA_site      2417
                     /gene="NANOG"
                     /note="major polyA site"
     regulatory      5159..5164
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NANOG"
                     /note="hexamer: AATAAA"
     polyA_site      5182
                     /gene="NANOG"
                     /note="major polyA site"
ORIGIN      
        1 ataaatctag agactccagg attttaacgt tctgctggac tgagctggtt gcctcatgtt
       61 attatgcagg caactcactt tatcccaatt tcttgatact tttccttctg gaggtcctat
      121 ttctctaaca tcttccagaa aagtcttaaa gctgccttaa ccttttttcc agtccacctc
      181 ttaaattttt tcctcctctt cctctatact aacatgagtg tggatccagc ttgtccccaa
      241 agcttgcctt gctttgaagc atccgactgt aaagaatctt cacctatgcc tgtgatttgt
      301 gggcctgaag aaaactatcc atccttgcaa atgtcttctg ctgagatgcc tcacacggag
      361 actgtctctc ctcttccttc ctccatggat ctgcttattc aggacagccc tgattcttcc
      421 accagtccca aaggcaaaca acccacttct gcagagaaga gtgtcgcaaa aaaggaagac
      481 aaggtcccgg tcaagaaaca gaagaccaga actgtgttct cttccaccca gctgtgtgta
      541 ctcaatgata gatttcagag acagaaatac ctcagcctcc agcagatgca agaactctcc
      601 aacatcctga acctcagcta caaacaggtg aagacctggt tccagaacca gagaatgaaa
      661 tctaagaggt ggcagaaaaa caactggccg aagaatagca atggtgtgac gcagaaggcc
      721 tcagcaccta cctaccccag cctttactct tcctaccacc agggatgcct ggtgaacccg
      781 actgggaacc ttccaatgtg gagcaaccag acctggaaca attcaacctg gagcaaccag
      841 acccagaaca tccagtcctg gagcaaccac tcctggaaca ctcagacctg gtgcacccaa
      901 tcctggaaca atcaggcctg gaacagtccc ttctataact gtggagagga atctctgcag
      961 tcctgcatgc agttccagcc aaattctcct gccagtgact tggaggctgc cttggaagct
     1021 gctggggaag gccttaatgt aatacagcag accactaggt attttagtac tccacaaacc
     1081 atggatttat tcctaaacta ctccatgaac atgcaacctg aagacgtgtg aagatgagtg
     1141 aaactgatat tactcaattt cagtctggac actggctgaa tccttcctct cccctcctcc
     1201 catccctcat aggatttttc ttgtttggaa accacgtgtt ctggtttcca tgatgcccat
     1261 ccagtcaatc tcatggaggg tggagtatgg ttggagccta atcagcgagg tttctttttt
     1321 tttttttttc ctattggatc ttcctggaga aaatactttt tttttttttt tttttgaaac
     1381 ggagtcttgc tctgtcgccc aggctggagt gcagtggcgc ggtcttggct cactgcaagc
     1441 tccgtctccc gggttcacgc cattctcctg cctcagcctc ccgagcagct gggactacag
     1501 gcgcccgcca cctcgcccgg ctaatatttt gtatttttag tagagacggg gtttcactgt
     1561 gttagccagg atggtctcga tctcctgacc ttgtgatcca cccgcctcgg cctccctaac
     1621 agctgggatt tacaggcgtg agccaccgcg ccctgcctag aaaagacatt ttaataacct
     1681 tggctgccgt ctctggctat agataagtag atctaatact agtttggata tctttagggt
     1741 ttagaatcta acctcaagaa taagaaatac aagtacaaat tggtgatgaa gatgtattcg
     1801 tattgtttgg gattgggagg ctttgcttat tttttaaaaa ctattgaggt aaagggttaa
     1861 gctgtaacat acttaattga tttcttaccg tttttggctc tgttttgcta tatcccctaa
     1921 tttgttggtt gtgctaatct ttgtagaaag aggtctcgta tttgctgcat cgtaatgaca
     1981 tgagtactgc tttagttggt ttaagttcaa atgaatgaaa caactatttt tcctttagtt
     2041 gattttaccc tgatttcacc gagtgtttca atgagtaaat atacagctta aacataaatc
     2101 tttgttaaat ttttggttgg ggtggagaag gaaattagct gaggacactg ctatcttaga
     2161 aatgcataga aatagctgag cgtggtggcc tatgcctgta gtcccagcta tttgggaggc
     2221 tgaggcagga gaatcgcttg aacctggtag gtggaggttg tagtgagcca atatcacacc
     2281 actgcactcc agcttgggcg acaagagcaa aactcccatc tcaaaaataa ataaataaat
     2341 aagtaaataa atgaataata aataaatatg ccagttttag aatgaatgaa taataaatat
     2401 gccagtttta gaaaaaaggg cacttacgtg cattgtacat gtgcatctac aaatgtaatg
     2461 ggttatttcc gtgtatgaat ctgtaattga tgctaacatt taaagtttca tcagaggcct
     2521 atatgtaagt aaattctaag gctagttgaa acagatttat gttctcacct taagagcagc
     2581 tacgcaggca tctgtgaatt atactaataa tcctttactg tccttttccc atcttgtgcc
     2641 tgccctaaat gtatctgatc tgagagcacg tcccttcact ctgaagcagc atgatgcagg
     2701 ttatcagagt ggttttttcc agtcttttct ctcattattt ccccaattgt ccaatgttac
     2761 taagactatc cagtctaatt ccaagtgttt tttttttgtt ttattttgtt tttttttaag
     2821 actggagtct tactctgttg cccaggctgg agtgtagtgg agcgatctcg gctcaccgca
     2881 acctccgcct cccgagttca agtgattctc ctgcctcagc ctcccaagta gctgggacta
     2941 caggggtgag ccaccatgcc cagctaattg tatttttagt agagacaggg tttcactatg
     3001 ttggccaggc tggtcttgaa ctcctgacct catgatccac cagccttggc ctcccaaagt
     3061 gttgggatta caggcgtgag ccactgcgcc tggcctatcg tttgtttttt tagacagtct
     3121 ctctctattg cccaggctgg agtgcagtgg cacaatcttg gctcactgca acctccgcct
     3181 cctgggttcg agcaattctc ctgcgtcagc ctcctaagta gctgggatta caggcaccca
     3241 acattacacc cggctaattt ttggtatttt tagtagagat ggggtttcac catgttggcc
     3301 agcctggtct cgaactcctg acctcaggtg atccacctga ctcggcctcc caaagtggtg
     3361 ggattatagg tgtgagccac cgtgtctggg caattttttt tttttttttt gagatggaac
     3421 ttcacttttg ttactcaggc tggagcacaa tgatgcaatc tctactcact gcaacctgca
     3481 cctcccaggt tcaagtgatt ctcttgcctc agcctttcga gtggctggta ttacagggat
     3541 ttgccactat gcccagctaa ttctttgtat ttagtagaga tggggtttca ccgtattgac
     3601 caggctggtc tcgaactcct gacctcaggt aatccaccct cctcggtctc ccaaagtgct
     3661 aggattacag gcatgagcca cagtgcccag ccttcttttt tattttttta agacgggctt
     3721 attctgctgc tcagactggg gcttagtggc accatttcac ctcactgtag cctcgacttc
     3781 ccggattcaa gcaatcctcc tgcctgagtc tctcaaatag cccagactat aggtgcacac
     3841 caccatgcca gctaactttt gtgtttttag gagagacggg gtttccccat gttggcctgg
     3901 ctagtctcaa actcctgagc tcaagcagtc taccagcctt ggcctcccga agtgctgaga
     3961 ttacaggcat gagtcaccag cctagccctg tattatgttt tctgaacttt ttttttttta
     4021 atcacccagt attaatcttc aacctcttgg caggtaagaa tttagagatc tatttggaat
     4081 aatctttaaa gtaacaataa taactacagt caaaagcagt aagattagtg ggccaggtgc
     4141 cgtggctcac acctgtaatc ccagcacttc gggagaccaa ggcaggtgga tcacctgagg
     4201 tcaggagttc aagtttagca tggccaatat ggcgaaaccc tgtctctact aaaaatacag
     4261 aaatttgcca gaggtcttgg tttgcgcctg taatctcagc tacttggaag gcggaggcag
     4321 gataatcact tgaacccggg aggttgcagt gaccccagat aatgccaccc cactccagcc
     4381 tgggcgacag agcgtcactg tctcaaaaat aaacaaaaat aataggagat taggcaaaaa
     4441 ggatagtaca aggctctctg tttaacattt cttcctttct ctagaaaatt ttcctttctt
     4501 ttcccaaaag gaggtgtgtg ttacttccaa tgctatattt tagtagcttt accatatttt
     4561 agtttttaaa attcattatt ttgatttctc agctttcatt ttcatttcag tttgtacctg
     4621 aaggagagaa gcatggattg aatttgcctg ggttttccga atttcataat ataaatttaa
     4681 aactcagaag tgggaggaat acaggccttg attagtgaat ttcaagctca gttctcgggg
     4741 tgaaggacaa ggttaattct actctgagta attctaccgt tcagaaaacc tggtgtgtct
     4801 acaggaaaaa atacaggctg tgactacaaa cctaaaagta aaggcaaaca actgccagac
     4861 agaggcagga aagtggggga gagcccttgg taaatattgc atgcctcctg gggcccccat
     4921 gggctttgac tcaaggggga gatgaaggga atttgttaaa gaaagaagtc actgggacag
     4981 acaaaagctt ccggcacagg aagttagttc gactaaggat accgaaatgt aggaatttga
     5041 atgggttcct gggtgtgagg ttgaagtgaa acttgattga aaagaagctg ctggtggtga
     5101 gtctctcctt aagctattcc tggcttctgg gtagttggtg taaactctgt ggtccttgaa
     5161 taaagtgtgc ttgagaattt ta
//
