LOCUS       NM_023018               3235 bp    mRNA    linear   PRI 29-JAN-2025
DEFINITION  Homo sapiens NAD kinase (NADK), transcript variant 1, mRNA.
ACCESSION   NM_023018
VERSION     NM_023018.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Praharaj,P.P., Li,Y., Mary,C., Soflaee,M.H., Ryu,K., Kim,D.,
            Tran,D.H., Dey,T., Tom,H.J., Rion,H., Gelin,M., Lemoff,A.,
            Zacharias,L.G., Patricio,J.S., Mathews,T.P., Chen,Z., Lionne,C.,
            Hoxhaj,G. and Labesse,G.
  TITLE     Cryo-EM structure and regulation of human NAD kinase
  JOURNAL   Sci Adv 11 (4), eads2664 (2025)
   PUBMED   39854463
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Meng,X., Peng,F., Yu,S., Chi,X., Wang,W. and Shao,S.
  TITLE     Knockdown of NADK promotes LUAD ferroptosis via NADPH/FSP1 axis
  JOURNAL   J Cancer Res Clin Oncol 150 (5), 228 (2024)
   PUBMED   38700533
  REMARK    GeneRIF: Knockdown of NADK promotes LUAD ferroptosis via NADPH/FSP1
            axis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3235)
  AUTHORS   Wu,T., Ding,K., Wang,C., Lin,G., Xie,C., Chen,X., Li,Q., Yu,F.,
            Mao,Y., Hong,W., Lu,L. and Li,S.
  TITLE     G-protein-coupled estrogen receptor 1 promotes peritoneal
            metastasis of gastric cancer through nicotinamide adenine
            dinucleotide kinase 1-mediated redox modulation
  JOURNAL   FASEB J 38 (3), e23449 (2024)
   PUBMED   38315451
  REMARK    GeneRIF: G-protein-coupled estrogen receptor 1 promotes peritoneal
            metastasis of gastric cancer through nicotinamide adenine
            dinucleotide kinase 1-mediated redox modulation.
REFERENCE   4  (bases 1 to 3235)
  AUTHORS   Schild,T., McReynolds,M.R., Shea,C., Low,V., Schaffer,B.E.,
            Asara,J.M., Piskounova,E., Dephoure,N., Rabinowitz,J.D., Gomes,A.P.
            and Blenis,J.
  TITLE     NADK is activated by oncogenic signaling to sustain pancreatic
            ductal adenocarcinoma
  JOURNAL   Cell Rep 35 (11), 109238 (2021)
   PUBMED   34133937
  REMARK    GeneRIF: NADK is activated by oncogenic signaling to sustain
            pancreatic ductal adenocarcinoma.
REFERENCE   5  (bases 1 to 3235)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 3235)
  AUTHORS   Stelzl,U., Worm,U., Lalowski,M., Haenig,C., Brembeck,F.H.,
            Goehler,H., Stroedicke,M., Zenkner,M., Schoenherr,A., Koeppen,S.,
            Timm,J., Mintzlaff,S., Abraham,C., Bock,N., Kietzmann,S.,
            Goedde,A., Toksoz,E., Droege,A., Krobitsch,S., Korn,B.,
            Birchmeier,W., Lehrach,H. and Wanker,E.E.
  TITLE     A human protein-protein interaction network: a resource for
            annotating the proteome
  JOURNAL   Cell 122 (6), 957-968 (2005)
   PUBMED   16169070
REFERENCE   7  (bases 1 to 3235)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   8  (bases 1 to 3235)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
REFERENCE   9  (bases 1 to 3235)
  AUTHORS   Magni,G., Amici,A., Emanuelli,M., Orsomando,G., Raffaelli,N. and
            Ruggieri,S.
  TITLE     Enzymology of NAD+ homeostasis in man
  JOURNAL   Cell Mol Life Sci 61 (1), 19-34 (2004)
   PUBMED   14704851
  REMARK    Review article
REFERENCE   10 (bases 1 to 3235)
  AUTHORS   Lerner,F., Niere,M., Ludwig,A. and Ziegler,M.
  TITLE     Structural and functional characterization of human NAD kinase
  JOURNAL   Biochem Biophys Res Commun 288 (1), 69-74 (2001)
   PUBMED   11594753
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK023114.1, BC001709.1, AL031282.1 and BM842058.1.
            
            On May 17, 2019 this sequence version replaced NM_023018.4.
            
            Summary: NADK catalyzes the transfer of a phosphate group from ATP
            to NAD to generate NADP, which in its reduced form acts as an
            electron donor for biosynthetic reactions (Lerner et al., 2001
            [PubMed 11594753]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes variant 1 isoform (1).
            Variants 1, 2 and 5 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK023114.1, SRR7346977.1267052.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467144 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000341426.9/ ENSP00000341679.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1433              AK023114.1         1-1433
            1434-2340           BC001709.1         1297-2203
            2341-3041           AL031282.1         63210-63910         c
            3042-3235           BM842058.1         1-194
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..3235
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /note="NAD kinase"
                     /db_xref="GeneID:65220"
                     /db_xref="HGNC:HGNC:29831"
                     /db_xref="MIM:611616"
     exon            1..182
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            183..401
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..1563
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /EC_number="2.7.1.23"
                     /note="isoform 1 is encoded by transcript variant 1;
                     poly(P)/ATP NAD kinase"
                     /codon_start=1
                     /product="NAD kinase isoform 1"
                     /protein_id="NP_075394.3"
                     /db_xref="CCDS:CCDS30565.1"
                     /db_xref="GeneID:65220"
                     /db_xref="HGNC:HGNC:29831"
                     /db_xref="MIM:611616"
                     /translation="MEMEQEKMTMNKELSPDAAAYCCSACHGDETWSYNHPIRGRAKS
                     RSLSASPALGSTKEFRRTRSLHGPCPVTTFGPKACVLQNPQTIMHIQDPASQRLTWNK
                     SPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKK
                     FCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFE
                     NFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGENGSQAAGLDMDVGKQ
                     AMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGAS
                     MIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRH
                     GDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG"
     exon            402..485
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            486..615
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            616..721
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            722..807
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            808..910
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1065
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1165
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1323
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1406
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..3235
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1932..1937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /note="hexamer: AATAAA"
     polyA_site      1952
                     /gene="NADK"
                     /gene_synonym="dJ283E3.1; NADK1"
                     /note="major polyA site"
ORIGIN      
        1 agtgggcggg cgcggcgcat tgctgctcgg cggcgccggc gccggggtcc gggcggccat
       61 ggggcaacag gcggccaggg cgccaagggc caggtagcga cggctggcgg cggcgcggcg
      121 gcggggcctg cgggctcggt ccggcatgta gacgcccccg caggcgcccg cggcagcgaa
      181 cggcatcagt gtttttctga ccgaagttct catttcctga caatggaaat ggaacaagaa
      241 aaaatgacca tgaataagga attgagtcca gacgcggctg cttactgctg ctcggcctgc
      301 cacggcgatg agacctggag ttacaaccac cccatccggg gccgggccaa gtctcgcagc
      361 ctgtctgcct cgcccgccct ggggagcacc aaggagttca ggaggacacg ctctcttcat
      421 gggccatgcc cggtgaccac ttttggacca aaggcctgtg tgctgcagaa cccccagacc
      481 atcatgcaca ttcaggaccc cgcgagccag cggctgacgt ggaacaagtc cccaaagagc
      541 gtccttgtca tcaagaagat gagagatgcc agcctactgc agccgttcaa ggagctctgc
      601 acgcacctca tggaggagaa catgatcgtg tatgtggaaa agaaagtgct agaagaccct
      661 gccatcgcca gcgatgaaag ctttggggca gtgaagaaga aattctgtac ctttcgagaa
      721 gattatgatg acatttccaa tcagatagac ttcatcatct gcctgggggg agacgggacg
      781 ctgctgtacg cttcctcgct tttccagggc agcgtccctc cggtcatggc cttccacctg
      841 ggctccctgg gcttcctgac cccattcagc tttgagaact ttcagtccca agttactcag
      901 gtgatagagg ggaacgcagc tgttgttctc cggagtcggc tgaaggtcag ggtggtgaag
      961 gagctccggg ggaagaagac ggccgtgcac aatgggctgg gtgagaacgg ctcgcaggct
     1021 gcaggcctgg acatggatgt cgggaagcag gccatgcagt accaggtcct gaatgaggtg
     1081 gtgattgaca gaggcccctc ctcctacctg tccaatgtgg atgtctacct ggacggacac
     1141 ctcatcacca cggtgcaggg cgacggagtg atcgtgtcca ccccgacggg cagcacggcg
     1201 tatgcggccg cggccggggc ctccatgatc caccccaacg tgccggccat catgatcacg
     1261 cccatctgcc cccactcgct gtccttccgg cccatcgtgg tccccgcagg ggtcgagctg
     1321 aagatcatgc tgtcacctga agcaaggaac acagcatggg tgtcctttga tggacggaag
     1381 agacaagaga tccgccatgg agacagcatc agcatcacta cctcatgcta cccgctcccc
     1441 tccatctgtg tgcgggaccc cgtgagcgac tggtttgaga gcctcgccca gtgcctgcat
     1501 tggaacgtcc ggaagaagca agcccacttc gaggaggagg aggaggagga ggaggagggc
     1561 taggtcaagc ccctatccag gcccgaatcc ttccgctgcc ctccaagcgc cctctgggga
     1621 cagaccaatc tgcgtgtgtc tgtgaccgcc tgtctcagtg gcacggccac ttcctttctg
     1681 tagctgggtt agagcctggg tctgcctttt gtccagatca gctgtttttt taaaatgtct
     1741 gacttttttt gcatttctaa agaagcgtga gaaatgggct gggagtgctt ctgtcctgct
     1801 gacaccccgc ggtgggtccc tggagcgcgg cctccagctg ccgcaatttc catgccagga
     1861 tatttttccg caaatcagtc ggttgaaatt cagaggagtc agaatgactc gacctgtcct
     1921 tcaatgttga taataaatgt ctcagccaaa aaccttcctt gagctgccat gcttttcccc
     1981 ttgacctgca cctcttcccc taaaacttct gcagggaagc ccctggcgga ggcgccattg
     2041 aaagcatggt cttgccagtg gctggcaagg cggttttgtt ctgctcagtt tctggagagg
     2101 gttggatgcg tcccctgcca tccagccctc cccgcttgag gccagcactg agtctgggac
     2161 actcagcggg aagggggctg gcatcgccag cgacccacac attcctcacg tagcttctgc
     2221 tcccaggaag gtagtttaaa tcctgtatat actttttaga gactctttta aactttctga
     2281 agtgctgatg tacatacttt ctcgtacaca cttttgtgaa gatttcaagg ggaagggagt
     2341 cgtctgccat tcaatgttta catttatgtt ctgcaagacg ctgtcctcag ggaccattag
     2401 gggaccattc tgttcagtgc gatcctgatg gtccgggaga tgagggtttc cggggctagt
     2461 gatcgtgatc ccttttattt gcaactgtaa tgagaatttt tcacactaac acagcgaggg
     2521 actcaacacg ctgattctcc tcctgcctct cccgtgagtc tccagcctgc ccagcaccgg
     2581 cagctgtgga gcacgtggat gctgcctacc ccggcgcccg cgtcttccac gggcacaggt
     2641 gtgtggaggc cgtggtcgga ccctggtgtc ctggttactg ctgcccgggt gtcttttttt
     2701 tgagtaactg ctctctgagt tttgcacacg aagttgccct catctgctgg agatcgataa
     2761 ggaaggcaca agacgttctc ctctgcccgt gaggagcttc ccgcagccgc ctggcccagc
     2821 ctgggcacgt tctccgaggc atgtgtctcc ctgctcaccc tcgtctgggc acctcagcat
     2881 ctgtggactt gagcgtccaa aaaccctgag tgtgattctg ggcagccggc ctggcttgaa
     2941 gtccgccatg accctgggca caggggaagc ccagccgtgg gcttaggaga gagggaccag
     3001 cgcccagcgt tagggctgga agacggcagt gttcagaatt ccagccgctc atctgaacac
     3061 agaaggtgtg aactgacctc taaagcagcg tgagatggga atgatctaga aaactttgga
     3121 tttttgaagt aaattttaat gtttcatatt aatttcttga aaatgtatta aatgtcattg
     3181 aaagccttat tacgcttttc agatcctttc aataaacaag acttgtagaa aataa
//
