LOCUS       NM_001365896            6387 bp    mRNA    linear   PRI 20-JUN-2024
DEFINITION  Homo sapiens nascent polypeptide associated complex subunit alpha
            (NACA), transcript variant 7, mRNA.
ACCESSION   NM_001365896 XM_011538389
VERSION     NM_001365896.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Song,D., Peng,K., Palmer,B.E. and Lee,F.S.
  TITLE     The ribosomal chaperone NACA recruits PHD2 to cotranslationally
            modify HIF-alpha
  JOURNAL   EMBO J 41 (22), e112059 (2022)
   PUBMED   36219563
  REMARK    GeneRIF: The ribosomal chaperone NACA recruits PHD2 to
            cotranslationally modify HIF-alpha.
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 6387)
  AUTHORS   Addison,W.N., Pellicelli,M. and St-Arnaud,R.
  TITLE     Dephosphorylation of the transcriptional cofactor NACA by the PP1A
            phosphatase enhances cJUN transcriptional activity and osteoblast
            differentiation
  JOURNAL   J Biol Chem 294 (20), 8184-8196 (2019)
   PUBMED   30948508
  REMARK    GeneRIF: These results suggest that PP1A dephosphorylates NACA at
            specific residues, impacting cJUN transcriptional activity and
            osteoblast differentiation and function.
REFERENCE   4  (bases 1 to 6387)
  AUTHORS   Shen,K., Gamerdinger,M., Chan,R., Gense,K., Martin,E.M., Sachs,N.,
            Knight,P.D., Schlomer,R., Calabrese,A.N., Stewart,K.L.,
            Leiendecker,L., Baghel,A., Radford,S.E., Frydman,J. and
            Deuerling,E.
  TITLE     Dual Role of Ribosome-Binding Domain of NAC as a Potent Suppressor
            of Protein Aggregation and Aging-Related Proteinopathies
  JOURNAL   Mol Cell 74 (4), 729-741 (2019)
   PUBMED   30982745
  REMARK    GeneRIF: NAC is a potent suppressor of aggregation and
            proteotoxicity of mutant PolyQ-expanded proteins associated with
            human diseases like Huntington's disease and spinocerebellar
            ataxias.
REFERENCE   5  (bases 1 to 6387)
  AUTHORS   Berkholz,J., Kuzyniak,W., Hoepfner,M. and Munz,B.
  TITLE     Overexpression of the skNAC gene in human rhabdomyosarcoma cells
            enhances their differentiation potential and inhibits tumor cell
            growth and spreading
  JOURNAL   Clin Exp Metastasis 31 (8), 869-879 (2014)
   PUBMED   25209525
  REMARK    GeneRIF: Low NACA expression is associated with rhabdomyosarcoma.
REFERENCE   6  (bases 1 to 6387)
  AUTHORS   Yotov,W.V., Moreau,A. and St-Arnaud,R.
  TITLE     The alpha chain of the nascent polypeptide-associated complex
            functions as a transcriptional coactivator
  JOURNAL   Mol Cell Biol 18 (3), 1303-1311 (1998)
   PUBMED   9488445
REFERENCE   7  (bases 1 to 6387)
  AUTHORS   Yotov,W.V. and St-Arnaud,R.
  TITLE     Differential splicing-in of a proline-rich exon converts alphaNAC
            into a muscle-specific transcription factor
  JOURNAL   Genes Dev 10 (14), 1763-1772 (1996)
   PUBMED   8698236
  REMARK    GeneRIF: A 220 kDa isoform of Naca is expressed in mouse muscle
            tissues
REFERENCE   8  (bases 1 to 6387)
  AUTHORS   Yotov,W.V. and St-Arnaud,R.
  TITLE     Mapping of the human gene for the alpha-NAC/1.9.2 (NACA/1.9.2)
            transcriptional coactivator to Chromosome 12q23-24.1
  JOURNAL   Mamm Genome 7 (2), 163-164 (1996)
   PUBMED   8835540
REFERENCE   9  (bases 1 to 6387)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
REFERENCE   10 (bases 1 to 6387)
  AUTHORS   Wiedmann,B., Sakai,H., Davis,T.A. and Wiedmann,M.
  TITLE     A protein complex required for signal-sequence-specific sorting and
            translocation
  JOURNAL   Nature 370 (6489), 434-440 (1994)
   PUBMED   8047162
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117378.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 15, 2018 this sequence version replaced XM_011538389.1.
            
            Summary: This gene encodes a protein that associates with basic
            transcription factor 3 (BTF3) to form the nascent
            polypeptide-associated complex (NAC). This complex binds to nascent
            proteins that lack a signal peptide motif as they emerge from the
            ribosome, blocking interaction with the signal recognition particle
            (SRP) and preventing mistranslocation to the endoplasmic reticulum.
            This protein is an IgE autoantigen in atopic dermatitis patients.
            Alternative splicing results in multiple transcript variants, but
            the full length nature of some of these variants, including those
            encoding very large proteins, has not been determined. There are
            multiple pseudogenes of this gene on different chromosomes.
            [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (7) contains an alternate segment
            and encodes a longer protein (isoform d) compared to variant 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN03568911,
                              SAMN03568912 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000454682.6/ ENSP00000403817.1
            RefSeq Select criteria    :: based on manual assertion, longest
                                         protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AC117378.8         64099-64135         c
            38-109              AC117378.8         63288-63359         c
            110-5698            AC117378.8         54707-60295         c
            5699-5784           AC117378.8         53438-53523         c
            5785-5862           AC117378.8         53198-53275         c
            5863-6009           AC117378.8         52373-52519         c
            6010-6138           AC117378.8         51898-52026         c
            6139-6261           AC117378.8         51622-51744         c
            6262-6387           AC117378.8         51263-51388         c
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.3"
     gene            1..6387
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /note="nascent polypeptide associated complex subunit
                     alpha"
                     /db_xref="GeneID:4666"
                     /db_xref="HGNC:HGNC:7629"
                     /db_xref="MIM:601234"
     exon            1..37
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     exon            38..109
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..6276
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /EC_number="5.3.99.3"
                     /note="isoform d is encoded by transcript variant 7;
                     nascent-polypeptide-associated complex alpha polypeptide;
                     alpha-NAC, muscle-specific form"
                     /codon_start=1
                     /product="nascent polypeptide-associated complex subunit
                     alpha isoform d"
                     /protein_id="NP_001352825.1"
                     /db_xref="CCDS:CCDS91708.1"
                     /db_xref="GeneID:4666"
                     /db_xref="HGNC:HGNC:7629"
                     /db_xref="MIM:601234"
                     /translation="MPGEATETVPATEQELPQPQAETAVLPMSSALSVTAALGQPGPT
                     LPPPCSPAPQQCPLSAANQASPFPSPSTIASTPLEVPFPQSSSGTALPLGTAPEAPTF
                     LPNLIGPPISPAALALASPMIAPTLKGTPSSSAPLALVALAPHSVQKSSAFPPNLLTS
                     PPSVAVAESGSVITLSAPIAPSEPKTNLNKVPSEVVPNPKGTPSPPCIVSTVPYHCVT
                     PMASIQSGVASLPQTTPTTTLAIASPQVKDTTISSVLISPQNPGSLSLKGPVSPPAAL
                     SLSTQSLPVVTSSQKTAGPNTPPDFPISLGSHLAPLHQSSFGSVQLLGQTGPSALSDP
                     TVKTISVDHSSTGASYPSQRSVIPPLPSRNEVVPATVAAFPVVAPSVDKGPSTISSIT
                     CSPSGSLNVATSFSLSPTTSLILKSSPNATYHYPLVAQMPVSSVGTTPLVVTNPCTIA
                     AAPTTTFEVATCVSPPMSSGPISNIEPTSPAALVMAPVAPKEPSTQVATTLRIPVSPP
                     LPDPEDLKNLPSSVLVKFPTQKDLQTVPASLEGAPFSPAQAGLTTKKDPTVLPLVQAA
                     PKNSPSFQSTSSSPEIPLSPEATLAKKSLGEPLPIGKPASSMTSPLGVNSSASVIKTD
                     SYAGPDSAGPLLKSSLITPTVAAFPLESADPAGVAPTTAKGTSTYTTTASPFLEGTVS
                     LAPKNHPVKEGTLTTLPLVPTASENCPVAPSPQNTCAPLATLVLAPEIPKSVPSPSLP
                     PAGTPPGTKKVDGISHTSALAPVASSPKECPTEDSGASATASSKGTLTYLADSPSPLG
                     VSVSPQTKRPPTKKGSAGPDTPIGNLSSPVSPVEASFLPENSLSFQGSKDSPATTHSP
                     TPPSPKGAPTPSAVTPLSPKGVTLPPKETPTPSVVNLPFPKEGPATPAPKQAPALSMT
                     SSSPKKARATPAPKGIPASPSPKGAPTPPAATPPSPKGGPATPSPKWAPTPPAATPPS
                     PKGGPATPSPKGAPTPPAATPPSPKGGPATPSPKGAPTPPAVTPPSPKGSPAATPFPK
                     GASTPPAATPPSPKGSPAATPLPKGAPTTPAATLPSPKGGPATPSLKGAPTPPAATPP
                     SPKGGPATPSPKGAPMPPAATPPSPKGGLATPPHKGAPTTPAATPPSPKGGLATPPPK
                     GAPTTPAATPPSPKGGLATPPPKGAPTTPAATPPSPKGGLATPSPKGAPTTPAATPPS
                     PKGGLATPSPKGAPTTPAATPPSPKGGLATPSPKGAPTTPAATPPSPKGGPATPPPKG
                     APTPPAATPPSLKGGLATPPHKGAPNPAVVTPPSPKGGPATSPPKGAPTPPAATPPSP
                     KGSPGTPPPKGAPTPPAVTPPSPKGTPTLPATTPSSKGGPTTPSSKEGPTPPAATPSH
                     KGGPAMTPPSPKRGPAIPSPKGDPTSPAVIPLSPKKAPATPVTREGAATPSKGDLTPP
                     AVTPVSLKKAPATSAPKGGPATPSSKGDPTLPAVTPPSPKEPPAPKQVATSSSPKKAP
                     ATPAPMGAPTLPAVIPSSPKEVPATPSSRRDPIAPTATLLSKKTPATLAPKEALIPPA
                     MTVPSPKKTPAIPTPKEAPATPSSKEASSPPAVTPSTYKGAPSPKELLIPPAVTSPSP
                     KEAPTPPAVTPPSPEKGPATPAPKGTPTSPPVTPSSLKDSPTSPASVTCKMGATVPQA
                     SKGLPAKKGPTALKEVLVAPAPESTPIITAPTRKGPQTKKSSATSPPICPDPSAKNGS
                     KGPLSTVAPAPLLPVQKDSSKTAKGKDASHSPKGPLAPPESKASTPLTAAAFEKVLPK
                     PESASVSAAPSPPVSLPLAPSPVPTLPPKQQFLPSSPGLVLESPSKPLAPADEDELLP
                     LIPPEPISGGVPFQSVLVNMPTPKSAGIPVPTPSAKQPVTKNNKGSGTESDSDESVPE
                     LEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVT
                     IRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNI
                     QENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIM
                     ELTM"
     exon            110..5698
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     exon            5699..5784
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     exon            5785..5862
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     exon            5863..6009
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     exon            6010..6138
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     exon            6139..6261
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     exon            6262..6387
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6355..6360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /note="hexamer: AATAAA"
     polyA_site      6387
                     /gene="NACA"
                     /gene_synonym="HSD48; NAC-alpha; NACA1; skNAC"
                     /note="major polyA site"
ORIGIN      
        1 ctttctgccg ccatcttggt tccgcgttcc ctgcacaaaa tgcccggcga agccacagaa
       61 accgtccctg ctacagagca ggagttgccg cagccccagg ctgagacagc tgtgctacct
      121 atgtcttcag ccttgagtgt cactgctgcc ttagggcagc ctggacctac cctcccccct
      181 ccttgctctc ctgccccaca acagtgccct ctctcagctg ctaaccaggc ttccccattc
      241 ccttccccct ctactattgc ctcgacccct ttagaagttc cttttcccca gtcatcctct
      301 ggaacagccc tacctttggg aactgcccct gaagccccaa ccttcctacc aaacctaata
      361 gggcctccca tctccccagc tgccttagct ctagcctctc ccatgatagc tccaactctg
      421 aaagggaccc cttcctcttc agctccctta gctctggttg ccctggctcc ccactcagtt
      481 cagaagagtt ctgcttttcc acctaacctt cttacttcac ctccttcagt ggctgtagct
      541 gagtcagggt cagtgataac tctgtcagct cccattgctc cctcagaacc aaagactaat
      601 cttaataaag ttccctctga ggtagtccct aatccaaaag gcacccccag ccctccatgt
      661 atagtcagta ctgttcctta ccactgtgtg actcccatgg cctctattca atctggagtg
      721 gcctcccttc ctcagacaac acccacaact accctagcca tcgcttcccc tcaagtcaaa
      781 gataccacca tttcctcagt tctgatttct ccacaaaacc caggaagcct cagcctgaag
      841 gggcctgtta gtccacctgc tgccttatct ctttcaactc agtctcttcc tgtggtgacc
      901 tcttctcaaa agactgcggg tcccaacacc cccccagatt ttcccatttc tctgggctct
      961 catcttgcac ctttacatca gagttctttt ggttctgtcc aacttttagg tcaaacaggt
     1021 cctagtgctt tgtcagaccc tacagtgaag accatttctg tagatcattc ttccacaggg
     1081 gcctcttatc cttctcagag atctgtaatt cctccccttc cttccagaaa tgaggtagtt
     1141 cctgctactg tggctgcctt tccagtggtg gctccatctg ttgacaaagg tccctctacc
     1201 atctctagca taacctgcag cccttctggc tccttaaatg tagctacctc tttttcatta
     1261 tctcctacaa cctctctcat tctcaaaagc tctcctaatg ccacttatca ttatccttta
     1321 gtggcccaaa tgcccgtttc ttctgttgga accaccccac ttgtggtgac taacccctgt
     1381 acaattgctg cagcacctac tactaccttt gaggtagcta cttgtgtttc tcctccaatg
     1441 tcatcaggtc ccataagtaa catagaacca acttcccctg ctgccttggt tatggcacct
     1501 gtggctccca aagagccttc tactcaagta gcaaccactc tgaggatacc agtctctcct
     1561 cctctgccag accctgaaga cctcaaaaat ctccccagtt cagtattggt taaatttcca
     1621 acacaaaaag acctccaaac tgtacctgcc tctcttgaag gagccccttt ctctccagcc
     1681 caagcaggac tcaccaccaa gaaagaccct actgtattac cgttagtcca ggcagcccct
     1741 aaaaattccc cttctttcca aagtacatcc tcttctccag agatacctct ttctcctgaa
     1801 gccaccctag caaagaaaag ccttggggag cctctcccta taggtaagcc agccagcagt
     1861 atgacctccc ctctgggtgt taactcctcg gcctctgtaa tcaagacaga ttcttatgca
     1921 ggcccagact ctgctggtcc gcttctcaaa agttctctca ttaccccaac agtggctgca
     1981 tttcctttgg aaagtgctga ccctgccggg gtggctccca caactgccaa aggtacctca
     2041 acttatacaa ctacagccag cccttttcta gaaggaactg tctctttagc tcctaaaaac
     2101 cacccagtta aggaaggtac tcttactact ttacccttgg ttcctacagc ttcagaaaat
     2161 tgccctgtgg ctccatcccc ccagaatacc tgtgctcctc tggctacctt agtgctggcc
     2221 cctgaaatcc caaagtctgt gccctcaccc tctcttcccc cagctgggac tcctccaggt
     2281 acaaaaaagg ttgatggtat ttctcatact tcagcattgg cacctgttgc ttcctctccc
     2341 aaagagtgcc caactgagga ctctggtgct tctgctactg catcttccaa aggaactctg
     2401 acttacctag ctgattcccc atctccttta ggggttagtg tgtctcctca gactaaaaga
     2461 cctccaacca agaagggttc tgctggccct gatactccta ttggaaatct ctcatcccct
     2521 gtttctccag ttgaagcttc atttcttcca gagaatagtc tttctttcca aggctctaaa
     2581 gactcaccag ccacgacgca ttctcccact cctccatccc ccaaaggggc ccctactccc
     2641 tcagctgtga ctcctctgtc tcccaaagga gtaacactac cccccaaaga gacccccact
     2701 ccttcagtgg tgaatctgcc cttccccaaa gagggtccag ctactccagc acccaaacag
     2761 gctcctgctc tatccatgac ttcttcctcc cccaaaaagg cccgagcaac tccagcccct
     2821 aaaggaatcc cagcttcccc atcccccaaa ggggccccca cacccccagc tgcaactcct
     2881 ccctccccta aaggaggccc agctacccca tccccgaaat gggcccccac acccccagct
     2941 gcaactcctc cctccccaaa aggaggtcca gctactccat cccccaaagg ggcccccaca
     3001 cccccagctg caactcctcc ctcccccaaa ggaggtccag ctactccatc ccccaaaggg
     3061 gcccccacac ccccagctgt gactcctccc tcccccaaag gaagtccagc agctacccca
     3121 ttccccaaag gggcatccac acccccagct gcaactcctc cctcccccaa aggaagtcca
     3181 gcagctaccc cactccccaa aggggccccc acaaccccag ctgcaactct tccctcccca
     3241 aaaggaggtc cagctacccc atccctcaag ggggccccca ctcccccagc tgcgactcct
     3301 ccctccccaa aaggaggccc agctacccca tcccccaaag gggcccccat gcccccagct
     3361 gcaactcctc cctccccaaa aggaggtcta gctaccccac cccacaaagg ggcacccaca
     3421 accccagctg caactcctcc ctccccaaaa ggaggtctag ctaccccacc cccaaaaggg
     3481 gcccccacaa ccccagctgc aactcctcct tccccaaaag gaggtctagc taccccaccc
     3541 ccaaaagggg cccccacaac cccagctgca actcctcctt ccccaaaagg aggtctagct
     3601 accccatccc ccaaaggggc ccccacaacc ccagctgcaa ctcctccctc cccaaaagga
     3661 ggtctagcta ccccatcccc caaaggggcc cccacaaccc cagctgcaac tcctccctcc
     3721 ccaaaaggag gtctggctac cccatccccc aaaggggccc ccacaacccc agctgcaact
     3781 cctccctccc caaaaggagg cccagctacc ccacccccca aaggggcccc cactccccca
     3841 gctgcaactc ctccctccct aaaaggaggt ctagctaccc caccccacaa aggggccccc
     3901 aatcccgcag ttgtaactcc tccctctcca aaaggaggcc cagctacctc accccccaaa
     3961 ggggccccca ctcctccagc tgcaactcct ccctccccaa aaggaagccc aggtacccca
     4021 ccccccaaag gggcccccac tcccccagct gtaactcctc cctcccctaa agggacccct
     4081 actctcccag ctacaactcc ctcctctaaa ggaggcccaa ctactccatc ctccaaagag
     4141 ggccccactc ccccagctgc aaccccctcc cacaaaggag gtcccgctat gactcctccc
     4201 tcccccaaaa gaggaccagc tatcccatct cccaaagggg accccacttc cccagcagtg
     4261 attcctctct cccccaaaaa ggctccagca actccagtca ccagagaagg cgcagccacc
     4321 ccatccaaag gagatctcac tcccccagca gtgactcctg tctccctcaa aaaggcccca
     4381 gcaacttcag cccccaaagg aggcccagct accccatcct ccaaagggga tcccaccctc
     4441 ccagcagtga ctcctccttc ccccaaggag cccccagccc ccaaacaagt tgccacttct
     4501 tcctctccca aaaaggcccc agcaactcca gcccccatgg gggcccccac tctgccagct
     4561 gtgattcctt cttcccccaa agaggtccca gctaccccat cctccagaag ggaccccatt
     4621 gccccaacag cgactcttct ctctaaaaag accccagcaa ctctagcccc caaagaggcc
     4681 ctcattcccc cagctatgac tgttccctcc cctaaaaaga ccccagcaat tccaaccccc
     4741 aaagaagccc cagctacccc atcctccaaa gaggcctcca gtcccccagc agtgactcct
     4801 tccacttaca aaggggcccc atcccccaaa gagctcctca ttccaccagc tgtgacttct
     4861 ccttccccca aagaggcacc tactcctcca gctgtgactc ctccatcccc cgaaaagggc
     4921 ccagcaactc cagcccccaa agggactccc acttccccac ctgtgactcc ttcctccctc
     4981 aaagactccc ctacttcccc agcttctgtc acatgtaaaa tgggggccac tgttcctcaa
     5041 gcatctaaag ggcttccagc aaagaaaggc cccacagctc tgaaagaagt acttgttgcc
     5101 ccagctccag aaagcacgcc aatcatcaca gctcccactc ggaaaggtcc acagaccaaa
     5161 aagagttctg ctacttcacc tcctatatgc ccagatccct cagctaagaa tggttctaaa
     5221 ggaccccttt ccacagtggc tccagcccct ctactccctg ttcagaaaga ctcttcaaag
     5281 acagcaaaag gcaaagatgc ttctcattcc ccaaagggcc ccttggctcc tcctgagtct
     5341 aaggcgtcca cccctctaac agcagctgcc tttgagaagg tccttcctaa acctgaatca
     5401 gcatctgtct ctgcagcacc ctccccacca gtctctctgc ctcttgctcc ctccccagtt
     5461 cccactctgc ctcctaaaca gcaatttctg ccgtcctctc ctgggctggt gttggaatca
     5521 ccctctaaac cccttgcccc tgctgatgag gatgagctgc tgcctctgat tcccccggaa
     5581 ccaatctctg ggggagtgcc tttccagtcg gtcctcgtca acatgcccac ccctaaatct
     5641 gctggaatcc ctgtcccaac cccctctgcc aagcaacctg ttacgaagaa caacaagggg
     5701 tctggaacag aatctgacag tgatgaatca gtaccagagc ttgaagaaca ggattccacc
     5761 caggcaacca cacaacaagc ccagctggcg gcagcagctg aaattgatga agaaccagtc
     5821 agtaaagcaa aacagagtcg gagtgaaaag aaggcacgga aggctatgtc caaactgggt
     5881 cttcggcagg ttacaggagt tactagagtc actatccgga aatctaagaa tatcctcttt
     5941 gtcatcacaa aaccagatgt ctacaagagc cctgcttcag atacttacat agtttttggg
     6001 gaagccaaga tcgaagattt atcccagcaa gcacaactag cagctgctga gaaattcaaa
     6061 gttcaaggtg aagctgtctc aaacattcaa gaaaacacac agactccaac tgtacaagag
     6121 gagagtgaag aggaagaggt cgatgaaaca ggtgtagaag ttaaggacat tgaattggtc
     6181 atgtcacaag caaatgtgtc gagagcaaag gcagtccgag ccctgaagaa caacagtaat
     6241 gatattgtaa atgcgattat ggaattaaca atgtaaccat atggaagcaa ctttttttgg
     6301 tgtctcaaag gagtaactgc agcttggttt gaaatttgta ctgtttctat cataaataaa
     6361 gttatggctt cttgttggat gaattca
//
