LOCUS       NM_018177               9720 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens NEDD4 binding protein 2 (N4BP2), transcript variant 1,
            mRNA.
ACCESSION   NM_018177 XR_109791 XR_112630 XR_113617
VERSION     NM_018177.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Leslie,E.J., Carlson,J.C., Shaffer,J.R., Buxo,C.J., Castilla,E.E.,
            Christensen,K., Deleyiannis,F.W.B., Field,L.L., Hecht,J.T.,
            Moreno,L., Orioli,I.M., Padilla,C., Vieira,A.R., Wehby,G.L.,
            Feingold,E., Weinberg,S.M., Murray,J.C. and Marazita,M.L.
  TITLE     Association studies of low-frequency coding variants in
            nonsyndromic cleft lip with or without cleft palate
  JOURNAL   Am J Med Genet A 173 (6), 1531-1538 (2017)
   PUBMED   28425186
  REMARK    GeneRIF: We found that a burden of low-frequency coding variants in
            N4BP2, CDSN, PRTG, and AHRR were associated with increased risk of
            Nonsyndromic cleft lip with or without cleft palate (NSCL/P) .
            Low-frequency variants in other genes were associated with
            decreased risk of NSCL/P. These results demonstrate that
            low-frequency variants contribute to the genetic etiology of NSCL/P
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   Jeong,E.Y., Kim,T.G. and Ban,C.
  TITLE     Modulated nicking endonuclease function by the N-terminal extended
            region of the smr domain in human Bcl-3 binding protein
  JOURNAL   Proteins 80 (1), 327-332 (2012)
   PUBMED   22072629
  REMARK    GeneRIF: the N-terminal extended region of the Smr domain in the
            native B3bp may function as a regulatory region for the nicking
            endonuclease
REFERENCE   3  (bases 1 to 9720)
  AUTHORS   Diercks,T., AB,E., Daniels,M.A., de Jong,R.N., Besseling,R.,
            Kaptein,R. and Folkers,G.E.
  TITLE     Solution structure and characterization of the DNA-binding activity
            of the B3BP-Smr domain
  JOURNAL   J Mol Biol 383 (5), 1156-1170 (2008)
   PUBMED   18804481
  REMARK    GeneRIF: The solution structure of the functionally active Smr
            domain of the Bcl3-binding protein (also known as Nedd4-binding
            protein 2), a protein with unknown function that lacks other
            domains present in MutS proteins, is determined.
REFERENCE   4  (bases 1 to 9720)
  AUTHORS   Oshikawa,M., Sugai,Y., Usami,R., Ohtoko,K., Toyama,S. and Kato,S.
  TITLE     Fine expression profiling of full-length transcripts using a
            size-unbiased cDNA library prepared with the vector-capping method
  JOURNAL   DNA Res 15 (3), 123-136 (2008)
   PUBMED   18487259
REFERENCE   5  (bases 1 to 9720)
  AUTHORS   Zheng,M.Z., Qin,H.D., Yu,X.J., Zhang,R.H., Chen,L.Z., Feng,Q.S. and
            Zeng,Y.X.
  TITLE     Haplotype of gene Nedd4 binding protein 2 associated with sporadic
            nasopharyngeal carcinoma in the Southern Chinese population
  JOURNAL   J Transl Med 5, 36 (2007)
   PUBMED   17626640
  REMARK    GeneRIF: These data suggest that haplotype blocks ATTA and GTTG of
            N4BP2 is correlation with the risk of sporadic nasopharyngeal
            carcinoma in the Southern Chinese population.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9720)
  AUTHORS   Watanabe,N., Wachi,S. and Fujita,T.
  TITLE     Identification and characterization of BCL-3-binding protein:
            implications for transcription and DNA repair or recombination
  JOURNAL   J Biol Chem 278 (28), 26102-26110 (2003)
   PUBMED   12730195
  REMARK    GeneRIF: B3BP is a 5'-polynucleotide kinase
REFERENCE   7  (bases 1 to 9720)
  AUTHORS   Murillas,R., Simms,K.S., Hatakeyama,S., Weissman,A.M. and
            Kuehn,M.R.
  TITLE     Identification of developmentally expressed proteins that
            functionally interact with Nedd4 ubiquitin ligase
  JOURNAL   J Biol Chem 277 (4), 2897-2907 (2002)
   PUBMED   11717310
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC098591.4, AK001542.1,
            BC126466.1, AC095057.3, AB371584.1 and AW135590.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018177.5.
            
            Summary: This gene encodes a protein containing a polynucleotide
            kinase domain (PNK) near the N-terminal region, and a Small MutS
            Related (Smr) domain near the C-terminal region. The encoded
            protein can bind to both B-cell leukemia/lymphoma 3 (BCL-3) and
            neural precursor cell expressed, developmentally downregulated 4,
            (Nedd4) proteins. This protein binds and hydrolyzes ATP, may
            function as a 5'-polynucleotide kinase, and has the capacity to be
            a ubiquitylation substrate. This protein may play a role in
            transcription-coupled DNA repair or genetic recombination.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            CDS exon combination :: AY267013.1, BC126466.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA1965299, SAMEA1968540 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261435.11/ ENSP00000261435.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                AC098591.4         106326-106379
            55-1300             AK001542.1         1-1246
            1301-2222           BC126466.1         937-1858
            2223-4451           AC098591.4         169418-171646
            4452-5786           BC126466.1         4088-5422
            5787-8312           AC095057.3         26154-28679
            8313-9719           AB371584.1         8330-9736
            9720-9720           AW135590.1         17-17               c
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p14"
     gene            1..9720
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /note="NEDD4 binding protein 2"
                     /db_xref="GeneID:55728"
                     /db_xref="HGNC:HGNC:29851"
                     /db_xref="MIM:619139"
     exon            1..181
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            182..278
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            279..621
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     CDS             393..5705
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     BCL-3-binding protein"
                     /codon_start=1
                     /product="NEDD4-binding protein 2 isoform 1"
                     /protein_id="NP_060647.2"
                     /db_xref="CCDS:CCDS3457.1"
                     /db_xref="GeneID:55728"
                     /db_xref="HGNC:HGNC:29851"
                     /db_xref="MIM:619139"
                     /translation="MPRRRKNLGGNPFRKTANPKEVVVSSVASREEPTTTLPSMGETK
                     VDQEELFTSISEIFSDLDPDVVYLMLSECDFKVENAMDCLLELSATDTKIEESSSQSF
                     VASENQVGAAESKIMEKRPEEESEDSKMDSFLDMQLTEDLDSLIQNAFEKLNSSPDDQ
                     VYSFLPSQDVNSFNDSSEFINPDSSNMTPIFSTQNMNLNGENLENSGSTLSLNPLPSH
                     SVLNESKCFIKDNTLALESNYPEDSLLSSSLNVASDSIAGCSSLNQKQKELLESECVE
                     AQFSEAPVDLDASEPQACLNLPGLDLPGTGGDQKSTRVSDVFLPSEGFNFKPHKHPEL
                     PTKGKDVSYCPVLAPLPLLLPPPPPPPMWNPMIPAFDLFQGNHGFVAPVVTTAAHWRS
                     VNYTFPPSVISHTSPTKVWRNKDGTSAYQVQETPVSQVVRKKTSYVGLVLVLLRGLPG
                     SGKSFLARTLQEDNPSGVILSTDDYFYINGQYQFDVKYLGEAHEWNQNRAKEAFEKKI
                     SPIIIDNTNLQAWEMKPYVALSQKHKYKVLFREPDTWWKFKPKELARRNIHGVSKEKI
                     TRMLEHYQRFVSVPIIMSSSVPEKIERIELCAYSCEDRSTSPRDDEDIISEKEENILS
                     LSLKHLEFTEEKNLDVTKETMLPENVAYLSNADLNKRRKEISDMNPSIQSALILETPH
                     MYFSDSESKLQATDKSENEQIEMVAVKGYSKTDTDSSMERVSPSTCCSENNQEDCDLA
                     NSGPLQNEKSSPGEIVEERATVTKKAFGKQKSKSTLEKFPRHELSNFVGDWPVDKTIG
                     QRTKRNRKTEKTSSVQSDKKYNYPQSHKLVNSVSVNTDCVQQRGSPHESVEDGRKSQC
                     DDASEPLNSYKYDAYKNIDKNSFNIMGDWPSSDSLAQREHRSRMPKTGLSEPNLEIGT
                     NDKMNEISLSTAHEACWGTSSQKLKTLGSSNLGSSEMLLSEMTCESQTCLSKKSHGQH
                     TSLPLTFTNSAPTVSGVVEPQTLAECQEQMPKRDPGKEVGMCTQTEPQDFALLWKIEK
                     NKISISDSIKVLTGRLDGFKPKVFNINTKSDVQEAIPYRVMYDKSTFVEESELTSADE
                     SENLNILCKLFGSFSLEALKDLYERCNKDIIWATSLLLDSETKLCEDTEFENFQKSCD
                     GSQIGPFSLGLNLKEIISQRGTLENSNSPVPEFSHGIGISNADSQSTCDAERGNSEQA
                     EMRAVTPENHESMTSIFPSAAVGLKNNNDILPNSQEELLYSSKQSFPGILKATTPKDM
                     SETEKNLVVTETGDNIHSPSHFSDIFNFVSSTSNLELNEEIYFTDSLEIKRNENFPKD
                     YVKFSDEEEFMNEDEKEMKEILMAGSSLSAGVSGEDKTEILNPTPAMAKSLTIDCLEL
                     ALPPELAFQLNELFGPVGIDSGSLTVEDCVVHIDLNLAKVIHEKWKESVMERQRQEEV
                     SCGKFMQDPSLVGHTGLDNPEQKSSQRTGKKLLKTLTASEMLPLLDHWNTQTKKVSLR
                     EIMSEEIALQEKHNLKRETLMFEKDCATKLKEKQLFKIFPAINQNFLVDIFKDHNYSL
                     EHTVQFLNCVLEGDPVKTVVAQEFVHQNENVTSHTGQKSKEKKPKKLKETEETPSELS
                     FQDFEYPDYDDYRAEAFLHQQKRMECYSKAKEAYRIGKKNVATFYAQQGTLHEQKMKE
                     ANHLAAIEIFEKVNASLLPQNVLDLHGLHVDEALEHLMRVLEKKTEEFKQNGGKPYLS
                     VITGRGNHSQGGVARIKPAVIKYLISHSFRFSEIKPGCLKVMLK"
     exon            622..1765
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1890
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..1979
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2056
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2212
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..4590
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            4591..4676
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            4677..4722
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            4723..4919
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            4920..5038
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            5039..5177
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            5178..5366
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            5367..5535
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            5536..5659
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     exon            5660..9720
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9701..9706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /note="hexamer: AATAAA"
     polyA_site      9720
                     /gene="N4BP2"
                     /gene_synonym="B3BP"
                     /note="major polyA site"
ORIGIN      
        1 agtgtctccc cgccgggcgc gctcgccgtg tctcccccgc ggccgcagct gcttgctagc
       61 cttgcgcggc gcgggagagc gcagtggcgc cggcgggaaa gggctgcgga cctgcggcgc
      121 cgcgttgtgc gttcgacgac gcggcaccgg cttcgacgcc ctctgcccgc tccagaagca
      181 gtaagaagac atgttggata acaagaagag gtgtagagtt tgcatatatc aactgtggcc
      241 ttaatgagca tgttgacagc atcgatgcta gacagacagt ctttttactg ctggattgtg
      301 caagatattt aaccctattt tgtttgaatt atgacttaaa tgtcaaacat cttaactaag
      361 aaaagggaaa cattttagtt ttggaagtca gaatgccaag gagaaggaaa aatcttgggg
      421 gaaatccttt tcggaagact gcaaacccta aggaagttgt cgtatccagt gttgctagtc
      481 gtgaggagcc aaccactact ctaccttcca tgggtgagac aaaagttgat caggaagaac
      541 tcttcaccag tatctcagag atattttctg atctggatcc tgatgtagtg tatttgatgc
      601 tttctgaatg tgatttcaaa gttgaaaatg ctatggattg tctattagaa ttatctgcca
      661 ctgataccaa gatagaagaa tcatcttcac aaagtttcgt tgcttctgag aaccaagtag
      721 gtgcagcaga aagtaaaata atggaaaaac gtcctgaaga agagagtgaa gattcaaaaa
      781 tggattcatt tttggacatg cagctaactg aagacctgga ttccttaata cagaatgctt
      841 ttgagaaatt gaactcttct cctgatgacc aagtatactc atttttgcct tcacaagatg
      901 ttaatagttt taatgactca agtgagttta taaatcctga ttcaagtaat atgactccca
      961 ttttttctac acaaaatatg aatttgaacg gtgaaaattt agagaattct ggttctactt
     1021 taagtttaaa cccattacct tcacattcag ttttgaacga gtccaagtgt tttataaagg
     1081 ataacacatt ggctttggaa agtaactacc cggaagattc tcttctcagt agttctttaa
     1141 atgtagcaag tgactccatc gcaggttgta gcagtctcaa tcaaaaacag aaagaacttt
     1201 tagaatctga gtgcgttgag gctcaattct ctgaagctcc tgtagatttg gatgccagtg
     1261 aacctcaggc ttgtttaaac cttccagggc ttgatttacc aggtacaggt ggggatcaga
     1321 aatctactcg ggtctctgat gtgtttctac cttccgaagg gttcaacttc aagccacaca
     1381 aacatcctga actgccaact aaggggaagg atgtgagtta ctgcccggta cttgctcctc
     1441 tcccattgct gttgcctcct ccgccacctc caccgatgtg gaatccaatg attcctgctt
     1501 ttgacctctt ccaaggaaac catggctttg tagctcctgt tgtaaccaca gctgcacact
     1561 ggagatctgt caactacaca tttccaccct cagttatttc tcacacttcc ccaacaaaag
     1621 tatggagaaa taaagatgga acaagtgctt atcaagtaca agaaacccca gtttctcagg
     1681 ttgtaagaaa gaagacatct tacgttggac tagttcttgt tcttctcaga ggtcttccgg
     1741 gatctggaaa atcttttttg gcaaggactt tgcaagagga taatccaagt ggagtcattc
     1801 ttagtactga tgattatttt tatataaatg gacagtacca gtttgatgta aagtacttag
     1861 gagaagcaca tgaatggaac cagaatcgtg caaaagaagc atttgagaag aagatatctc
     1921 ctataattat agataataca aacctacagg catgggaaat gaaaccatat gttgctttgt
     1981 ctcagaaaca caaatataaa gtcctttttc gggaaccaga cacatggtgg aagtttaaac
     2041 caaaggaact tgcaaggcgt aacattcatg gggtaagcaa agaaaaaata acaagaatgt
     2101 tggaacatta tcaacgtttt gtttcagtgc caataattat gagttcttcg gttccagaga
     2161 aaattgaacg tattgagttg tgtgcatatt cttgtgagga tagaagcact agcccaagag
     2221 acgatgaaga tattatctct gaaaaagaag aaaatatttt atctttatct ttgaagcatc
     2281 tagagttcac tgaagagaag aatcttgatg taaccaaaga aacaatgtta cctgagaatg
     2341 ttgcatatct ctctaatgca gatttaaaca aaagaagaaa agaaataagt gatatgaatc
     2401 ctagcattca aagtgcttta attctggaaa ctccacacat gtatttttct gactctgaaa
     2461 gcaaactaca ggcaacagac aaaagtgaaa acgagcaaat agaaatggtg gctgtaaaag
     2521 ggtatagtaa aactgacaca gatagttcta tggagagagt atcacctagt acttgctgta
     2581 gtgaaaataa tcaagaagac tgtgatcttg caaatagtgg accacttcaa aatgaaaaat
     2641 cctcacctgg tgaaatagtg gaagaaagag caacagtaac gaaaaaagcc tttgggaaac
     2701 aaaaaagcaa atcgactttg gaaaagttcc caagacatga gctatcaaat tttgttggtg
     2761 actggccagt tgataagact attggtcaga ggacaaaaag gaacagaaaa actgaaaaaa
     2821 cttcatccgt acaaagcgac aaaaagtata attaccctca gtcacacaaa ttagttaaca
     2881 gtgtatctgt gaatacagat tgtgtccagc aacgaggatc tccacatgaa agtgtagagg
     2941 atggcagaaa gtcacagtgt gatgatgctt cagagccact caatagctat aaatatgatg
     3001 cttataaaaa tattgacaaa aactcattca acattatggg tgactggcct tcatctgatt
     3061 ctttagctca gagggaacac agatcaagaa tgccaaagac tggtttaagt gagcccaacc
     3121 tagaaattgg aacaaatgac aaaatgaatg aaatatcctt atctacagca catgaggcct
     3181 gttggggcac aagctctcaa aaactaaaga cattgggtag ctccaatcta ggaagttctg
     3241 aaatgctgct cagtgaaatg acctgtgaga gtcagacttg tctaagtaaa aagagtcatg
     3301 ggcaacacac atcgttgcct cttactttta ccaatagtgc accaactgtt tctggagtag
     3361 tagaaccaca aacgttagct gaatgtcaag agcaaatgcc taagagagac cctggaaaag
     3421 aagtaggcat gtgcacccag actgaaccac aggattttgc tcttttatgg aaaatagaaa
     3481 agaataaaat tagcatttca gattctatca aagtattaac aggaagatta gatggattta
     3541 agccgaaagt tttcaatatt aacacaaaat cagacgttca agaagcaatt ccatatagag
     3601 taatgtatga taaaagcacg tttgttgaag aaagtgagct taccagtgca gatgaatctg
     3661 aaaatcttaa cattctttgt aaactgtttg gatccttttc attagaagcc ctgaaagact
     3721 tatatgagag gtgcaataaa gatattattt gggccacaag ccttttgttg gattctgaaa
     3781 ctaagttatg tgaggataca gagtttgaga atttccaaaa atcgtgtgat ggatcacaaa
     3841 ttgggccttt ttctctgggg ttgaatttga aagaaattat tagccaaaga ggaactttag
     3901 agaattctaa ttctcctgtg ccagagttta gccatgggat tggtattagt aacgctgact
     3961 cacagtctac ttgtgatgca gaaagaggaa actcagagca ggcggaaatg agagctgtca
     4021 ctcctgaaaa ccatgaatcg atgacaagta tatttcccag tgctgctgtg ggtctaaaga
     4081 ataataatga catacttcct aacagccagg aagaactttt atatagcagt aagcagtcct
     4141 ttccaggtat tctaaaagct actactccta aagatatgag tgaaacagaa aaaaacctag
     4201 tagtcacaga gactggagac aacatacatt ctccttcaca tttctctgat atttttaact
     4261 ttgtatctag tacttcaaat cttgaattaa atgaagaaat ttattttact gattctcttg
     4321 aaataaagag aaatgaaaat tttccaaagg attatgtgaa attttcagat gaagaagaat
     4381 ttatgaatga agatgagaag gaaatgaagg aaattctaat ggcaggaagt agtttatcag
     4441 ctggagttag tggggaagat aaaaccgaga tattgaatcc cactccagcg atggccaaat
     4501 ctctgaccat agactgtctg gaattggcat taccccctga actggctttt caacttaatg
     4561 aattatttgg tcctgttggt attgattcag ggtctctaac agttgaagat tgtgtggttc
     4621 atatagatct gaatctggcg aaagtgattc atgagaaatg gaaagaatct gtaatggagc
     4681 gacaaagaca agaagaggtg tcttgtggca agtttatgca agatccttcc ttggttggac
     4741 atactgggct tgataatcct gaacaaaaat catctcagag aacaggcaaa aaattactga
     4801 agactttaac agcatctgaa atgctacctt tattggatca ttggaatact caaactaaaa
     4861 aagtatcact cagagaaata atgtcagaag aaattgcctt acaggaaaaa cataatttga
     4921 aaagggagac ccttatgttt gaaaaagatt gtgccactaa actaaaggag aagcagctct
     4981 ttaagatatt tccagccatt aaccaaaatt ttctggtgga cattttcaag gaccacaact
     5041 attcattaga acacacagtg caatttctta actgtgttct tgaaggggac cctgtaaaaa
     5101 cagttgtagc ccaagagttt gttcaccaaa atgagaatgt cacatctcat actggccaga
     5161 agtctaaaga gaaaaagcca aagaaattaa aagagactga agaaacacca agtgaactgt
     5221 ctttccagga ctttgagtac ccagactatg atgactacag agcagaggct ttccttcacc
     5281 aacagaagag gatggagtgc tacagcaagg ccaaagaagc ttatcggata gggaaaaaaa
     5341 atgtcgccac cttttatgcc cagcagggta ctcttcatga gcagaagatg aaagaagcca
     5401 atcaccttgc tgccatagag atctttgaga aagtcaatgc ttcgctgctg ccacagaatg
     5461 ttttagacct ccatgggctg catgtggatg aagctctaga acatttgatg agagttttag
     5521 agaagaagac tgaagaattt aagcagaacg gtgggaagcc ctatttgtct gtgattacgg
     5581 ggagaggaaa ccacagccag ggaggagttg ctcgcatcaa accagctgtc attaagtacc
     5641 tcataagcca tagcttcagg ttctctgaaa ttaaaccagg gtgcttgaaa gtcatgctaa
     5701 agtaaaataa acatccttga attagaagta tgaaggtttg taggttaaaa ttacttttat
     5761 ttgcagtaat tcagtcaatt ctaccctaaa gatgtgtttt attataaata atattcaatt
     5821 atgaaacaaa aaaattatga tgtttttcaa aatgcaagtg aggtttgatt ttttactgaa
     5881 tcaaatgcaa atgttacctg ttaaagatat tacagagaaa ttttattgat tacaaaatat
     5941 gtaagaaaat tatcagctac agttttaaag tttaaaatgc tctgattgtt tctcagaaaa
     6001 ggttaccttt gtattaattg ttgtatgaca tttagtaatg tcctaaaagt cttttgtgag
     6061 aggtatttaa agtgctttga gacctgattc atgcccccca aagggtggta ttaaaagagg
     6121 aaaaaggaca tagtagtggt agtaaagaaa aaaaggagat ctaacaaaaa cacttgaata
     6181 ttttttattt tcaaaactaa acacataaaa tgcttttatt ttgccatatc ctacaggaag
     6241 gaagacatgc ttttgccctt tcttctgctt tgtatatgac cttgatcttt taccttttgt
     6301 tctgttcttc tgtgtgcatt ttatggaaag aaagttgggt ctgagattat attgtaattt
     6361 tatactttct atagacagaa aggttttcag agaaaaagaa atggttaaat atcttccaga
     6421 tgttgctgcc ttatcgaaat gcttctagaa ctgtctgtat aagtcgtatt tcagacagta
     6481 ctaaatactt atattttatt tttgaggcat gtaattttaa aactgcagta ggttaaaatt
     6541 ctattaaaca aatatagaaa atattttgtt tataattggc ctggtgtggt ggcccacgcc
     6601 tgtaatccca gcactttggg aggccgaggt aggtggatcg cctgaggtca gaagttcaag
     6661 accagcctgg ccaacatggt gaatccccgt ctctattaaa aatacaaaaa ttagctgggt
     6721 gtggtggcgt gtgcctgtaa tcccagctac ttgggaggct gaggcaggag aaccgcttaa
     6781 acctgggagg tggaggttgc agtgacccga gatcgagatc acaccactgc actcagcctg
     6841 ggtgacagag caagactccg tctccaaaaa aaaaaaagaa aaaaaagaga aaatattttg
     6901 gttataatca taagaagaat ccttacaatt taaaatattt tcacttttat tttgaaaaag
     6961 agccaacttt ctcaaaggaa gctgtcatta acttctgttg tttggttagc taataaattc
     7021 tgttagtatg caagttaaac ctgtgtaaag gaggttataa tgtggtaagg tataattttc
     7081 caatatgaga caatggctta aatttgcctt tggagatgga gaatattttc aacatacata
     7141 attttaacaa atagttttgt tgctttttgt taagcttaaa aaaaaatttg gatcagaaat
     7201 tataggattg agctggtacc aaaagaatcc taaagtacag aatggttttc caaaagagca
     7261 gacatttcaa tgaaaataaa accccaaaac atttttttta tatatcatga ttgcatgctt
     7321 ttaaaataag gaggcttatc attgaatttt agttccataa aaatgaaaat acatgtttat
     7381 tatttagaaa tggttagtga ctggtattga agtattattc acacttatgt ttgtaataat
     7441 ttatttaaag aatgccaaca gtttagcaca cttttgtaat ctctagaaca gttctataca
     7501 ttaaaaataa acattaaaaa taaagcagtt cctttaaaca acttactatg aaactataat
     7561 tgttaatgaa ataagatatt cctatgtata cattagtatg atttaagggt atgaaaaaca
     7621 gtgctaaaat gtggagttaa ttggaatgtg tgcagctgca tactcacttt ctgtgttttt
     7681 atatttcctt acctagtttt aaaaatcatc acttttcggc acttcagcta gacttatttc
     7741 aatattataa ttatgattaa tgaaggagca gtcagaagta gaggtgtggt attccgtggg
     7801 cagaagtcaa atgaaaaaag ctatattgtt tacttgttta ctatatcaat gttaatatta
     7861 tgccttattt tgataaatca aatagtatat cttaatcatt gttgataaat atacaggcaa
     7921 agtttgagca tcatatgtat ttttatgaag ctgcttaatt ccaaattgcc tcccacccca
     7981 ctatctagca tagtttaagc aacacataat tttgaaagaa aagtttgtaa tccctccttt
     8041 cttttttttg cccatttcta aatcctgtat gcccgcagta aagatagttt ggggatttta
     8101 aaagttttct ctattaccca tttctaaatc ctgtatgtca aagatagttt ggagattata
     8161 aaaattttca tgtattggtg taggttgagt atccctaatg agaaaattca aaatctgaaa
     8221 tgcttcaaaa tctgaaattt ttcgagcaca gacatgatgc tcaaaggaaa tactcacaga
     8281 gcattttaca tttgtaggtt agcgatactc aacaagtaaa tataatgcac atgttctaaa
     8341 atatcccccc aaaccccagc aatccaaaac acttctggtc ctaagcattt tgagtagggg
     8401 atactcactc aacctgtata tttgtgctaa tacatgactc attagaatga ttctttgtaa
     8461 acttaatatt ttaaaagtac agcacttctg tagtatggaa ggtttcagta ataattatat
     8521 tcattcagta gtctcttacc attatctccc agatggaaaa agaggactaa tgtggaaacc
     8581 ccagagggtg tccagttgga ccagggagat attagacact taacagtatt ttcagtctgt
     8641 ccatctcttt attccaatgt gagaaatgga agtgtttttt tttttacgtt tattggctct
     8701 tcatatttct ctacattatt tttaatgtgc agtttcttca attggttagt atttccatac
     8761 tatttgcaac tttatggcct ttaaatatag gacatattat atagcagaaa ttttgacttt
     8821 aaatcctctt gagtagtata ttttgagaag aaaagctata ctgctcttct ggatggtttc
     8881 catcctttat ttaggtcttt tctttttgaa ttcaagtgtt ttgtatgctt agaaagtaga
     8941 catgtataat attgagatcg gttatttctg agctggaaat tggaaacttt ggaaactcag
     9001 gaaattgctc tgacaatgtt ttaactgctc tcaatttaag aaaatgacga aatgtataaa
     9061 aaagacaaaa ataacgtgtg ctgttttttc caagtgcttt ttctaagtgc ttttccattg
     9121 tgcaatgagg tgaagtttgg taatttttcg gtgtagtagt taaatattgc tcaattttta
     9181 tttacatgta aagaaaacag atttaaatgt ttatgtggcc aaaaggtgtc atttaaaagg
     9241 taaaataagt ttatgtagaa tgtatgttca tggtgcttat ttttaaaatg taattcaagt
     9301 ttacagtatt acttaatgct tctttacaga atttaataga gaaacaaggc tagaacacat
     9361 ctacatcctg aagagccgtt tataacttca tattatatga tgacaaagtt cattattttc
     9421 cttaaagttg agcaattgac ttttatggtc caatgatgaa cttattatta ataaatgatt
     9481 gagttaactg tgaggcttct cattaaaata caatattgca gctatcagtt ggagaatata
     9541 ttataaaatt ttcagacagt atatcagaaa aatgttttta tttgtactgt atagaaaatg
     9601 taattttgct gttaactctg tactttttaa attgaaaatg ttttataaat ttgcttttaa
     9661 attttcttat gaagccattt gcaaattaca tacttaattt aataaaatac tttagccaca
//
