LOCUS       NM_001018100            2398 bp    mRNA    linear   PRI 18-JUL-2024
DEFINITION  Homo sapiens myocardial zonula adherens protein (MYZAP), transcript
            variant 1, mRNA.
ACCESSION   NM_001018100
VERSION     NM_001018100.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Pieperhoff,S., Rickelt,S., Heid,H., Claycomb,W.C., Zimbelmann,R.,
            Kuhn,C., Winter-Simanowski,S., Kuhn,C., Frey,N. and Franke,W.W.
  TITLE     The plaque protein myozap identified as a novel major component of
            adhering junctions in endothelia of the blood and the lymph
            vascular systems
  JOURNAL   J Cell Mol Med 16 (8), 1709-1719 (2012)
   PUBMED   21992629
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Rickelt,S., Kuhn,C., Winter-Simanowski,S., Zimbelmann,R., Frey,N.
            and Franke,W.W.
  TITLE     Protein myozap--a late addition to the molecular ensembles of
            various kinds of adherens junctions
  JOURNAL   Cell Tissue Res 346 (3), 347-359 (2011)
   PUBMED   22160502
REFERENCE   3  (bases 1 to 2398)
  AUTHORS   Huo,L., Wen,W., Wang,R., Kam,C., Xia,J., Feng,W. and Zhang,M.
  TITLE     Cdc42-dependent formation of the ZO-1/MRCKbeta complex at the
            leading edge controls cell migration
  JOURNAL   EMBO J 30 (4), 665-678 (2011)
   PUBMED   21240187
REFERENCE   4  (bases 1 to 2398)
  AUTHORS   Garcia-Mayoral,M.F., Martinez-Moreno,M., Albar,J.P.,
            Rodriguez-Crespo,I. and Bruix,M.
  TITLE     Structural basis for the interaction between dynein light chain 1
            and the glutamate channel homolog GRINL1A
  JOURNAL   FEBS J 277 (10), 2340-2350 (2010)
   PUBMED   20412299
REFERENCE   5  (bases 1 to 2398)
  AUTHORS   Seeger,T.S., Frank,D., Rohr,C., Will,R., Just,S., Grund,C.,
            Lyon,R., Luedde,M., Koegl,M., Sheikh,F., Rottbauer,W., Franke,W.W.,
            Katus,H.A., Olson,E.N. and Frey,N.
  TITLE     Myozap, a novel intercalated disc protein, activates serum response
            factor-dependent signaling and is required to maintain cardiac
            function in vivo
  JOURNAL   Circ Res 106 (5), 880-890 (2010)
   PUBMED   20093627
REFERENCE   6  (bases 1 to 2398)
  AUTHORS   Roginski,R.S., Goubaeva,F., Mikami,M., Fried-Cassorla,E., Nair,M.R.
            and Yang,J.
  TITLE     GRINL1A colocalizes with N-methyl D-aspartate receptor NR1 subunit
            and reduces N-methyl D-aspartate toxicity
  JOURNAL   Neuroreport 19 (17), 1721-1726 (2008)
   PUBMED   18849881
REFERENCE   7  (bases 1 to 2398)
  AUTHORS   Roginski,R.S., Mohan Raj,B.K., Birditt,B. and Rowen,L.
  TITLE     The human GRINL1A gene defines a complex transcription unit, an
            unusual form of gene organization in eukaryotes
  JOURNAL   Genomics 84 (2), 265-276 (2004)
   PUBMED   15233991
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC025271.7, AC090651.3 and
            KF456061.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001018100.4.
            
            Summary: This gene encodes a protein that is abundantly expressed
            in cardiac tissue. The encoded protein localizes to intercalated
            discs in cardiomyocytes and functions as an activator of
            Rho-dependent serum-response factor signaling. Alternative splicing
            results in multiple transcript variants. Readthrough transcription
            also exists between this gene and the neighboring downstream gene
            POLR2M (polymerase (RNA) II (DNA directed) polypeptide M) and is
            represented with GeneID: 145781. [provided by RefSeq, Mar 2014].
            
            Transcript Variant: This variant (1, also known as Gup1) represents
            the longer transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.30813.1,
                                           SRR3476690.980702.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267853.10/ ENSP00000267853.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-206               AC025271.7         157230-157435
            207-293             AC025271.7         169595-169681
            294-449             AC025271.7         183359-183514
            450-542             AC025271.7         186934-187026
            543-651             AC025271.7         191105-191213
            652-656             AC090651.3         7597-7601
            657-809             AC090651.3         11411-11563
            810-935             AC090651.3         14143-14268
            936-1064            AC090651.3         15322-15450
            1065-1144           AC090651.3         19404-19483
            1145-1250           AC090651.3         21149-21254
            1251-1334           AC090651.3         43159-43242
            1335-1435           KF456061.1         142-242
            1436-2398           AC090651.3         66111-67073
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.3"
     gene            1..2398
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /note="myocardial zonula adherens protein"
                     /db_xref="GeneID:100820829"
                     /db_xref="HGNC:HGNC:43444"
                     /db_xref="MIM:614071"
     exon            1..206
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..1532
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; myocardium-enriched zonula adherens protein;
                     GRINL1A upstream protein; myocardial intercalated disc
                     protein; myocardium-enriched zonula occludens-1-associated
                     protein; myocardium-enriched ZO1-associated protein;
                     GRINL1A complex locus upstream"
                     /codon_start=1
                     /product="myocardial zonula adherens protein isoform 1
                     precursor"
                     /protein_id="NP_001018110.1"
                     /db_xref="CCDS:CCDS10162.1"
                     /db_xref="GeneID:100820829"
                     /db_xref="HGNC:HGNC:43444"
                     /db_xref="MIM:614071"
                     /translation="MLRSTSTVTLLSGGAARTPGAPSRRANVCRLRLTVPPESPVPEQ
                     CEKKIERKEQLLDLSNGEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWSTSQLKEEMN
                     YIKDVRATLEKVRKRMYGDYDEMRQKIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
                     NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRNLQQTYEASMDKLREKQRQLEVAQV
                     ENQLLKMKVESSQEANAEVMREMTKKLYSQYEEKLQEEQRKHSAEKEALLEETNSFLK
                     AIEEANKKMQAAEISLEEKDQRIGELDRLIERMEKERHQLQLQLLEHETEMSGELTDS
                     DKERYQQLEEASASLRERIRHLDDMVHCQQKKVKQMVEEIESLKKKLQQKQLLILQLL
                     EKISFLEGENNELQSRLDYLTETQAKTEVETREIGVGCDLLPSQTGRTREIVMPSRNY
                     TPYTRVLELTMKKTLT"
     exon            207..293
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            294..449
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            450..542
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            543..656
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            657..809
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            810..935
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1064
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1144
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1145..1250
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1334
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1435
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..2398
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2370..2375
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /note="hexamer: AATAAA"
     polyA_site      2398
                     /gene="MYZAP"
                     /gene_synonym="CMD2K; GCOM1; Gup; MYOZAP"
                     /note="major polyA site"
ORIGIN      
        1 cttcgcggtg cagctgaggc tgcaagtagc cggcgccgtc ccgcgtcgcc cccgcgcagg
       61 gcgggccccg cacgcttatt ctgcccggga ggaacgccgg cgtccagccc gctaccgacc
      121 gccgctgcgg gatgctgcgc tccacgtcca cggtcaccct gctctcgggc ggcgccgcca
      181 ggacgcccgg ggcgcccagc aggagggcaa atgtttgcag actacggctg accgtacctc
      241 ctgagagtcc agttcctgag caatgtgaaa agaagattga gagaaaagag cagcttcttg
      301 acctgagcaa tggagaacct accaggaaac ttcctcaggg tgttgtttat ggtgtggtgc
      361 gaagatcaga tcaaaatcag cagaaagaaa tggtggtgta tgggtggtcc accagtcagc
      421 tgaaagaaga gatgaactac atcaaagatg tgagagccac tttggaaaag gtgagaaagc
      481 gaatgtatgg agactatgat gagatgagac agaagattcg acagctcacc caggaactat
      541 cagtttccca tgctcagcag gagtatctgg agaatcacat ccaaacccag tcgtctgccc
      601 tggatcgttt taatgccatg aactcagcct tggcatcaga ttccattggc ctgcagaaaa
      661 ccctcgtgga tgtgactttg gaaaacagca acattaagga tcaaatcaga aatctgcagc
      721 agacgtatga agcatccatg gacaagctga gggaaaagca gaggcagttg gaggtagcgc
      781 aagttgaaaa ccagctgcta aaaatgaagg tggaatcgtc ccaagaagcc aatgctgagg
      841 tgatgcgaga gatgaccaag aagctgtaca gccagtatga ggagaagctg caggaagaac
      901 agaggaagca cagtgctgag aaggaggctc ttttggaaga aaccaatagt tttctgaaag
      961 cgattgaaga agccaataaa aagatgcaag cagcagagat cagcctagag gagaaagacc
     1021 agaggatcgg ggagctggac aggctgattg agcgcatgga aaaggaacgt catcaactgc
     1081 aacttcaact cctagaacat gaaacagaaa tgtctgggga gttaactgat tctgacaagg
     1141 aaaggtatca gcagttggag gaggcatcag ccagcctccg tgagcggatc agacacctag
     1201 atgacatggt gcattgccag cagaagaaag tcaagcagat ggtcgaggag attgaatcat
     1261 taaagaaaaa gttgcaacag aaacagctct taatactgca gcttttagaa aagatatctt
     1321 tcttagaagg agagaataat gaactacaaa gcaggttgga ctatttaaca gaaacccagg
     1381 ccaagaccga agtggaaacc agagagatag gagtgggctg tgatcttcta cccagccaaa
     1441 caggcaggac tcgtgaaatt gtgatgcctt ctaggaacta caccccatac acaagagtcc
     1501 tggagttaac catgaagaaa actctgactt aggcactcag aggcatacac tttttacaga
     1561 tggacaaaag ctctggaacc ctgtggcttc aaatcctttg ggaagggtga ctgttgtttc
     1621 ccctacacac agtgtaagcc ggaatgggaa tcgctgaggc tctgatccac ttctaagaca
     1681 ggaaggaaag tgaaggcaga gtgagcaggt aagagaggga tatacaaggt cacatttcag
     1741 acacccactc ggcataccct gccgtactgc atcatcattt gttttctttg tagacactga
     1801 aatcctatca ggaggattcc ttcacaatgt attttatttg ctagactttg gttgggaggg
     1861 aaaaggacat taatttgaag tttcatgtta ttcatgccag gattgtttga tagagcatga
     1921 aggttttgtt tacccataaa agtattagag gcagcgtttc tctgatacag agaggcctgt
     1981 ccacaagaag catgggcacc cagccaaact tgaacctgga agggagggtt cccggcctgc
     2041 aggtgctctt tcctcttggt cccaagcatc tgtgcagggt cgtgggagcc acactgagag
     2101 acttgtgtgg gccagacaag cttcattctg atgcgctagt cccttggttt aatttgtgcc
     2161 ttatgctttc attggaccag ctgaaatcac tgtatttatt caactagtga tttttttttc
     2221 tttctcactt taacttaaag agaattttat atgtcttgga aatttaataa tttagtgttc
     2281 tcagtatcaa ttggtgtttt tgttaaacga atgaatcatc tgttcatgca tgctctactt
     2341 tgatattata acctatgtca catgtgttta ataaatacca tatattttgt tctactaa
//
