LOCUS       NM_003803               5707 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens myomesin 1 (MYOM1), transcript variant 1, mRNA.
ACCESSION   NM_003803
VERSION     NM_003803.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Hang,C., Song,Y., Li,Y., Zhang,S., Chang,Y., Bai,R., Saleem,A.,
            Jiang,M., Lu,W., Lan,F. and Cui,M.
  TITLE     Knockout of MYOM1 in human cardiomyocytes leads to myocardial
            atrophy via impairing calcium homeostasis
  JOURNAL   J Cell Mol Med 25 (3), 1661-1676 (2021)
   PUBMED   33452765
  REMARK    GeneRIF: Knockout of MYOM1 in human cardiomyocytes leads to
            myocardial atrophy via impairing calcium homeostasis.
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 5707)
  AUTHORS   Pernigo,S., Fukuzawa,A., Beedle,A.E.M., Holt,M., Round,A.,
            Pandini,A., Garcia-Manyes,S., Gautel,M. and Steiner,R.A.
  TITLE     Binding of Myomesin to Obscurin-Like-1 at the Muscle M-Band
            Provides a Strategy for Isoform-Specific Mechanical Protection
  JOURNAL   Structure 25 (1), 107-120 (2017)
   PUBMED   27989621
  REMARK    GeneRIF: Crystal structure of the obscurin(-like-1):myomesin
            complex reveals a trans-complementation mechanism whereby an
            incomplete immunoglobulin-like domain assimilates an
            isoform-specific myomesin interdomain sequence.
REFERENCE   4  (bases 1 to 5707)
  AUTHORS   Kravchenko,I.V., Furalyov,V.A., Chatziefthimiou,S., Wilmanns,M. and
            Popov,V.O.
  TITLE     Induction of insulin-like growth factor 1 splice forms by
            subfragments of myofibrillar proteins
  JOURNAL   Mol Cell Endocrinol 399, 69-77 (2015)
   PUBMED   25152160
  REMARK    GeneRIF: individual subfragments of titin and myomesin composed of
            Fn type III and Ig-like domains can activate expression of two
            IGF-1 splice forms in cultured myoblasts
REFERENCE   5  (bases 1 to 5707)
  AUTHORS   Blandin,G., Marchand,S., Charton,K., Daniele,N., Gicquel,E.,
            Boucheteil,J.B., Bentaib,A., Barrault,L., Stockholm,D., Bartoli,M.
            and Richard,I.
  TITLE     A human skeletal muscle interactome centered on proteins involved
            in muscular dystrophies: LGMD interactome
  JOURNAL   Skelet Muscle 3 (1), 3 (2013)
   PUBMED   23414517
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5707)
  AUTHORS   Speel,E.J., van der Ven,P.F., Albrechts,J.C., Ramaekers,F.C.,
            Furst,D.O. and Hopman,A.H.
  TITLE     Assignment of the human gene for the sarcomeric M-band protein
            myomesin (MYOM1) to 18p11.31-p11.32
  JOURNAL   Genomics 54 (1), 184-186 (1998)
   PUBMED   9806852
REFERENCE   7  (bases 1 to 5707)
  AUTHORS   Obermann,W.M., Plessmann,U., Weber,K. and Furst,D.O.
  TITLE     Purification and biochemical characterization of myomesin, a
            myosin-binding and titin-binding protein, from bovine skeletal
            muscle
  JOURNAL   Eur J Biochem 233 (1), 110-115 (1995)
   PUBMED   7588733
REFERENCE   8  (bases 1 to 5707)
  AUTHORS   Vinkemeier,U., Obermann,W., Weber,K. and Furst,D.O.
  TITLE     The globular head domain of titin extends into the center of the
            sarcomeric M band. cDNA cloning, epitope mapping and immunoelectron
            microscopy of two titin-associated proteins
  JOURNAL   J Cell Sci 106 (Pt 1), 319-330 (1993)
   PUBMED   7505783
REFERENCE   9  (bases 1 to 5707)
  AUTHORS   Cirino,A.L. and Ho,C.
  TITLE     Hypertrophic Cardiomyopathy Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301725
REFERENCE   10 (bases 1 to 5707)
  AUTHORS   Grove,B.K., Kurer,V., Lehner,C., Doetschman,T.C., Perriard,J.C. and
            Eppenberger,H.M.
  TITLE     A new 185,000-dalton skeletal muscle protein detected by monoclonal
            antibodies
  JOURNAL   J Cell Biol 98 (2), 518-524 (1984)
   PUBMED   6537951
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AJ621424.1, BC116184.1 and
            AI221348.1.
            
            On Sep 19, 2019 this sequence version replaced NM_003803.3.
            
            Summary: The giant protein titin, together with its associated
            proteins, interconnects the major structure of sarcomeres, the M
            bands and Z discs. The C-terminal end of the titin string extends
            into the M line, where it binds tightly to M-band constituents of
            apparent molecular masses of 190 kD (myomesin 1) and 165 kD
            (myomesin 2). This protein, myomesin 1, like myomesin 2, titin, and
            other myofibrillar proteins contains structural modules with strong
            homology to either fibronectin type III (motif I) or immunoglobulin
            C2 (motif II) domains. Myomesin 1 and myomesin 2 each have a unique
            N-terminal region followed by 12 modules of motif I or motif II, in
            the arrangement II-II-I-I-I-I-I-II-II-II-II-II. The two proteins
            share 50% sequence identity in this repeat-containing region. The
            head structure formed by these 2 proteins on one end of the titin
            string extends into the center of the M band. The integrating
            structure of the sarcomere arises from muscle-specific members of
            the superfamily of immunoglobulin-like proteins. Alternatively
            spliced transcript variants encoding different isoforms have been
            identified. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript, and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ621424.1, AK125322.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356443.9/ ENSP00000348821.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1215              AJ621424.1         141-1355
            1216-2700           BC116184.1         1096-2580
            2701-3072           AJ621424.1         2841-3212
            3073-3957           BC116184.1         2665-3549
            3958-5695           AJ621424.1         4098-5835
            5696-5707           AI221348.1         2-13                c
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.31"
     gene            1..5707
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /note="myomesin 1"
                     /db_xref="GeneID:8736"
                     /db_xref="HGNC:HGNC:7613"
                     /db_xref="MIM:603508"
     exon            1..166
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            167..484
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..5252
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /note="isoform a is encoded by transcript variant 1;
                     myomesin (M-protein) 1 (190kD); myomesin 1 (skelemin)
                     185kDa; 190 kDa titin-associated protein; 190 kDa
                     connectin-associated protein; EH-myomesin; myomesin family
                     member 1; myomesin 1, 185kDa"
                     /codon_start=1
                     /product="myomesin-1 isoform a"
                     /protein_id="NP_003794.3"
                     /db_xref="CCDS:CCDS45824.1"
                     /db_xref="GeneID:8736"
                     /db_xref="HGNC:HGNC:7613"
                     /db_xref="MIM:603508"
                     /translation="MSLPFYQRCHQHYDLSYRNKDVRSTVSHYQREKKRSAVYTQGST
                     AYSSRSSAAHRRESEAFRRASASSSQQQASQHALSSEVSRKAASAYDYGSSHGLTDSS
                     LLLDDYSSKLSPKPKRAKHSLLSGEEKENLPSDYMVPIFSGRQKHVSGITDTEEERIK
                     EAAAYIAQRNLLASEEGITTSKQSTASKQTTASKQSTASKQSTASKQSTASRQSTASR
                     QSVVSKQATSALQQEETSEKKSRKVVIREKAERLSLRKTLEETETYHAKLNEDHLLHA
                     PEFIIKPRSHTVWEKENVKLHCSIAGWPEPRVTWYKNQVPINVHANPGKYIIESRYGM
                     HTLEINGCDFEDTAQYRASAMNVKGELSAYASVVVKRYKGEFDETRFHAGASTMPLSF
                     GVTPYGYASRFEIHFDDKFDVSFGREGETMSLGCRVVITPEIKHFQPEIQWYRNGVPL
                     SPSKWVQTLWSGERATLTFSHLNKEDEGLYTIRVRMGEYYEQYSAYVFVRDADAEIEG
                     APAAPLDVKCLEANKDYIIISWKQPAVDGGSPILGYFIDKCEVGTDSWSQCNDTPVKF
                     ARFPVTGLIEGRSYIFRVRAVNKMGIGFPSRVSEPVAALDPAEKARLKSRPSAPWTGQ
                     IIVTEEEPSEGIVPGPPTDLSVTEATRSYVVLSWKPPGQRGHEGIMYFVEKCEAGTEN
                     WQRVNTELPVKSPRFALFDLAEGKSYCFRVRCSNSAGVGEPSEATEVTVVGDKLDIPK
                     APGKIIPSRNTDTSVVVSWEESKDAKELVGYYIEASVAGSGKWEPCNNNPVKGSRFTC
                     HGLVTGQSYIFRVRAVNAAGLSEYSQDSEAIEVKAAIGGGVSPDVCPALSDEPGGLTA
                     SRGRVHEASPPTFQKDALLGSKPNKPSLPSSSQNLGQTEVSKVSETVQEELTPPPQKA
                     APQGKSKSDPLKKKTDRAPPSPPCDITCLESFRDSMVLGWKQPDKIGGAEITGYYVNY
                     REVIDGVPGKWREANVKAVSEEAYKISNLKENMVYQFQVAAMNMAGLGAPSAVSECFK
                     CEEWTIAVPGPPHSLKCSEVRKDSLVLQWKPPVHSGRTPVTGYFVDLKEAKAKEDQWR
                     GLNEAAIKNVYLKVRGLKEGVSYVFRVRAINQAGVGKPSDLAGPVVAETRPGTKEVVV
                     NVDDDGVISLNFECDKMTPKSEFSWSKDYVSTEDSPRLEVESKGNKTKMTFKDLGMDD
                     LGIYSCDVTDTDGIASSYLIDEEELKRLLALSHEHKFPTVPVKSELAVEILEKGQVRF
                     WMQAEKLSGNAKVNYIFNEKEIFEGPKYKMHIDRNTGIIEMFMEKLQDEDEGTYTFQL
                     QDGKATNHSTVVLVGDVFKKLQKEAEFQRQEWIRKQGPHFVEYLSWEVTGECNVLLKC
                     KVANIKKETHIVWYKDEREISVDEKHDFKDGICTLLITEFSKKDAGIYEVILKDDRGK
                     DKSRLKLVDEAFKELMMEVCKKIALSATDLKIQSTAEGIQLYSFVTYYVEDLKVNWSH
                     NGSAIRYSDRVKTGVTGEQIWLQINEPTPNDKGKYVMELFDGKTGHQKTVDLSGQAYD
                     EAYAEFQRLKQAAIAEKNRARVLGGLPDVVTIQEGKALNLTCNVWGDPPPEVSWLKNE
                     KALASDDHCNLKFEAGRTAYFTINGVSTADSGKYGLVVKNKYGSETSDFTVSVFIPEE
                     EARMAALESLKGGKKAK"
     exon            485..625
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            626..965
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1123
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1216
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1217..1305
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1368
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1533
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1534..1695
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1837
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..2037
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2094
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2095..2219
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2403
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2578
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2579..2700
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2988
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3185
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3186..3312
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3313..3497
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3498..3612
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3613..3769
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3770..3876
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3877..3921
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3922..4058
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4059..4203
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4204..4263
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4264..4331
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4332..4445
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4446..4533
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4534..4572
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4573..4678
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4679..4842
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4843..4879
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4880..4902
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4903..4958
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     exon            4959..5707
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5674..5679
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /note="hexamer: AATAAA"
     polyA_site      5707
                     /gene="MYOM1"
                     /gene_synonym="SKELEMIN"
                     /note="major polyA site"
ORIGIN      
        1 gactgcctga ccacatgcat tatttttgaa ctggccccca gggtcctgtc tttagccctg
       61 cctcttaaca ttgtcttgga ttcaacctag gtggagtctt cattctctca tccagctcct
      121 cggcctcatc ggaacatttc caccaccatt actcctttgg aaactggtgg cccggttcct
      181 tcaaggggca caggatgtct ttgccttttt atcagaggtg ccaccagcac tatgatctca
      241 gctaccgcaa caaggacgtg cgcagcaccg tgagtcacta ccagcgggag aagaaacgct
      301 ccgccgtcta cacccagggc tccacggcct acagcagccg ctcctccgcc gcgcaccgcc
      361 gggagtccga ggccttccgt cgggcgtccg cctcctcctc ccagcagcag gcctcgcagc
      421 acgccctgag ctctgaagtc agtcggaagg cagcctcagc ctacgattat ggctcctccc
      481 atggacttac agattccagt ctgctgttag atgattattc atccaagttg agccccaaac
      541 caaagagagc caagcacagc ctactgtctg gagaagagaa agaaaatttg cccagtgact
      601 acatggtacc cattttctca ggacgtcaaa agcatgtcag tggaattact gatacggaag
      661 aagaaagaat taaagaagct gctgcttata tagcccagag gaatcttctt gctagtgagg
      721 aaggaatcac aacatctaaa cagtccacgg catccaagca gaccacggca tctaagcagt
      781 ccacggcatc caagcagtcc acagcatcca agcagtccac ggcatccagg cagtccacgg
      841 catccaggca gtctgtggtt tccaaacagg ccacatccgc tcttcaacag gaagaaactt
      901 ctgaaaagaa gtcaaggaaa gttgtgattc gagaaaaggc agaacgcctg tccctgagga
      961 aaacattaga agaaaccgag acatatcatg ccaagctgaa tgaagaccat cttctccatg
     1021 ctcctgagtt tatcattaaa cctcgctccc acacggtttg ggagaaggag aatgtaaaat
     1081 tgcattgctc catagcaggc tggccagaac ctcgtgtcac gtggtataaa aaccaggtgc
     1141 caataaatgt ccatgcaaac cctggaaagt atattattga gagtcgatat gggatgcaca
     1201 ctctggagat taatggatgt gattttgaag atacagctca gtaccgggcc tcggcgatga
     1261 atgttaaagg agagctttcg gcatatgctt cagttgtggt aaaaaggtat aagggagagt
     1321 ttgatgagac tcgcttccac gctggggctt ccaccatgcc cctcagcttt ggtgtgaccc
     1381 catatggtta tgcatcccgg tttgagatcc actttgatga caaatttgat gtgtcttttg
     1441 ggagagaggg agagacaatg agtctaggct gtcgtgttgt catcactcct gaaattaaac
     1501 atttccagcc agagatccag tggtacagaa acggagtacc tctttctcca tcaaaatggg
     1561 tgcaaacact ttggagtgga gagcgggcaa cgctgacatt ttcccatctc aacaaagaag
     1621 atgaaggcct ctatacaatc cgtgtacgga tgggagaata ttatgaacaa tatagtgctt
     1681 atgtctttgt tcgagatgct gatgcagaga ttgaaggagc cccagctgct cccttggatg
     1741 tgaagtgctt ggaggccaac aaagattata tcatcatctc ctggaaacag ccagctgtcg
     1801 atggagggag tcctattctc ggatatttta ttgataagtg tgaggtgggc acagatagct
     1861 ggtcgcagtg caatgacaca cctgtgaagt ttgctcgttt tcctgtcact ggattgatcg
     1921 aaggtcgttc ctatatcttc cgagttcgag ctgtgaataa aatgggaata ggtttcccat
     1981 ctcgagtttc cgagcccgtg gctgctctgg atccggctga gaaagctaga cttaaaagtc
     2041 gcccctcagc accctggact ggacagatca ttgttactga agaggaacct tcagagggta
     2101 ttgtgcctgg ccccccgaca gacctctctg tcactgaggc cacccggagc tatgtggtgc
     2161 tcagctggaa gccccctggc cagcgtggtc atgagggcat tatgtacttt gtggaaaagt
     2221 gtgaggcagg aacagaaaac tggcagcgag tgaacacgga gctccctgtg aagtctcccc
     2281 gctttgctct gtttgacttg gccgagggga aatcctactg tttccgtgtc cgctgttcta
     2341 attctgcagg agttggtgag ccctcagagg caacggaggt gactgtggta ggggacaaac
     2401 ttgatatccc caaggctcct ggcaaaatca tcccaagcag aaacacagac acctcagtgg
     2461 tagtttcgtg ggaggagtcc aaagatgcca aagagctggt cgggtactac atagaggcga
     2521 gcgttgctgg ctctggcaag tgggagccct gtaacaacaa ccccgtgaag ggctcacgat
     2581 tcacttgtca tggattagtg actggtcaga gttatatttt ccgggtcaga gcagtcaatg
     2641 cagctggact tagtgaatat tcccaggatt cagaagctat tgaagtcaaa gctgctattg
     2701 ggggaggagt gtctccagat gtgtgtcccg cactgagcga tgagcctggt ggactaaccg
     2761 cctccagggg gcgcgtgcat gaagcctccc cgccaacctt ccagaaagat gctttgcttg
     2821 gcagcaaacc taacaaacct tcactaccca gtagctctca aaacctgggc caaacagaag
     2881 tgagtaaagt aagtgaaaca gttcaggaag agcttacccc gccaccacag aaagcggctc
     2941 ctcaggggaa aagtaagtct gaccccctga aaaagaagac agacagagca ccaccatctc
     3001 caccctgtga tatcacctgt cttgaaagtt ttcgtgactc aatggttctt ggatggaagc
     3061 aaccagataa gattggaggg gcagaaatta ctggctatta tgtgaactat cgcgaggtca
     3121 ttgatggggt accaggaaaa tggagagaag ccaatgtcaa ggctgtcagt gaggaggcat
     3181 acaagattag caacttgaag gaaaacatgg tgtatcagtt ccaagtggca gccatgaaca
     3241 tggctgggct gggcgcgccc tccgcagtaa gcgaatgctt caaatgtgaa gagtggacca
     3301 tcgccgtccc aggaccaccg cacagtctca agtgtagtga agtcaggaaa gactcactgg
     3361 ttctccagtg gaagccgcca gtccactccg ggcggactcc ggtcactggt tacttcgtgg
     3421 acttgaagga ggccaaggcc aaagaagacc agtggcgagg gctcaatgag gcggctatta
     3481 aaaacgtata cctgaaggtt cgaggcctca aggagggcgt cagctacgtg ttccgtgttc
     3541 gagccataaa ccaggcggga gttgggaagc catctgacct tgctggccct gttgtggcag
     3601 agacccgtcc aggaaccaaa gaggttgttg taaatgtgga tgatgatgga gtcatttcat
     3661 tgaacttcga gtgtgataag atgactccaa agtccgagtt ctcctggtcc aaagattatg
     3721 tatccactga ggactctcca cgattggaag tcgaaagcaa gggcaacaag acgaaaatga
     3781 ccttcaaaga ccttgggatg gatgacttgg gtatttactc ttgcgatgta acagacactg
     3841 atggaatagc atcaagctac ttaatagatg aggaagaatt gaaacgttta cttgctctca
     3901 gccatgaaca caagttccca actgtcccag ttaaatcaga gttggcagtt gaaattttgg
     3961 agaaaggcca ggtccggttt tggatgcagg ctgagaaact gtctggcaat gccaaagtca
     4021 actacatatt taacgagaag gaaatttttg aaggcccgaa atataaaatg catattgacc
     4081 gaaacactgg catcatcgaa atgttcatgg aaaagctaca ggatgaggat gagggaacgt
     4141 acactttcca gcttcaagat ggaaaagcaa ctaaccattc tactgttgtt ctcgttggag
     4201 atgttttcaa aaagctccag aaagaagctg aattccagcg gcaagaatgg atcaggaaac
     4261 aaggtcctca ctttgttgag tatttgagct gggaagtgac tggtgaatgt aatgtactat
     4321 tgaaatgcaa ggtggcaaat attaagaagg agactcatat tgtgtggtac aaagatgaga
     4381 gggagatatc agtggatgaa aagcatgact ttaaggatgg tatatgtacc ctgcttataa
     4441 cagagttttc caagaaagat gctgggattt atgaagttat cctgaaagat gaccgaggaa
     4501 aagataagag cagactgaag cttgtggatg aagcctttaa ggaactgatg atggaagtat
     4561 gcaaaaaaat agctttgtct gctacagacc tgaaaatcca gagcacagcc gagggcatcc
     4621 aactgtactc ttttgtaact tactatgtgg aggatttgaa agttaactgg tcccacaatg
     4681 ggtccgccat taggtactca gacagagtta agaccggggt cactggagag cagatctggc
     4741 tacaaatcaa cgagcccacc ccgaatgaca aagggaagta tgtcatggag ctctttgatg
     4801 gcaaaactgg acatcagaag acagtggatc tctctggaca agcatacgat gaggcctatg
     4861 ctgaattcca gaggttgaaa caagctgcca ttgccgagaa aaatcgtgcc cgggtgttgg
     4921 gaggtctccc agacgtggtc accatccagg aggggaaggc ccttaatctc acttgcaacg
     4981 tgtggggaga cccgcctccg gaggtgtcgt ggttgaagaa cgagaaggcc ctggcctcag
     5041 acgaccactg caacctcaag ttcgaggctg ggaggaccgc gtacttcacc atcaacggcg
     5101 tgagcaccgc tgactcgggc aaatacgggc tggttgtgaa gaacaagtat ggctcggaga
     5161 ccagcgactt caccgtcagc gtgttcatcc cagaggagga ggcgaggatg gccgccttgg
     5221 agtccctgaa aggtggcaag aaggccaagt gaccggaggt gcgaggagag ccagccggcc
     5281 tgtgtgactt gggtgtgaat ggtttgggtt aaggatgaga cgtcttcatg ctttctcctc
     5341 cctattattt tctggcttga ggggaaaata atgtcaggtc tttcactcat ataaaaaagc
     5401 accaactaat gacactttaa ttgtttttct ttatctacaa aattatgtgt taagaaaata
     5461 ccattcatag catgaagatt aggaaacagt tttaaggaga agacttgaat gaagttggag
     5521 ggacattgaa tgatggtcag agggcagacg aatgtgtcgt ggggcgaatt gggatttgct
     5581 gcagctgtga agccatggcc gtgtctcgtg tgttgttaca gaggtgatgt gcttttcgac
     5641 gggcgcctcg tggcttggaa cctcctctgt atgaataaac agttttcacg tctgtcctct
     5701 tccccga
//
