LOCUS       NM_001146312            8565 bp    mRNA    linear   PRI 04-AUG-2024
DEFINITION  Homo sapiens myocardin (MYOCD), transcript variant 1, mRNA.
ACCESSION   NM_001146312
VERSION     NM_001146312.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Bankell,E., Liu,L., van der Horst,J., Rippe,C., Jepps,T.A.,
            Nilsson,B.O. and Sward,K.
  TITLE     Suppression of smooth muscle cell inflammation by myocardin-related
            transcription factors involves inactivation of TANK-binding kinase
            1
  JOURNAL   Sci Rep 14 (1), 13321 (2024)
   PUBMED   38858497
  REMARK    GeneRIF: Suppression of smooth muscle cell inflammation by
            myocardin-related transcription factors involves inactivation of
            TANK-binding kinase 1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8565)
  AUTHORS   Javanshir,E., Ebrahimi,Z.J., Mirzohreh,S.T., Ghaffari,S.,
            Banisefid,E., Alamdari,N.M. and Roshanravan,N.
  TITLE     Disparity of gene expression in coronary artery disease: insights
            from MEIS1, HIRA, and Myocardin
  JOURNAL   Mol Biol Rep 51 (1), 712 (2024)
   PUBMED   38824221
  REMARK    GeneRIF: Disparity of gene expression in coronary artery disease:
            insights from MEIS1, HIRA, and Myocardin.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8565)
  AUTHORS   Sakai,T., Choo,Y.Y., Mitsuhashi,S., Ikebe,R., Jeffers,A., Idell,S.,
            Tucker,T.A. and Ikebe,M.
  TITLE     Myocardin regulates fibronectin expression and secretion from human
            pleural mesothelial cells
  JOURNAL   Am J Physiol Lung Cell Mol Physiol 326 (4), L419-L430 (2024)
   PUBMED   38349126
  REMARK    GeneRIF: Myocardin regulates fibronectin expression and secretion
            from human pleural mesothelial cells.
REFERENCE   4  (bases 1 to 8565)
  AUTHORS   Liu,L., Kryvokhyzha,D., Rippe,C., Jacob,A., Borreguero-Munoz,A.,
            Stenkula,K.G., Hansson,O., Smith,C.W.J., Fisher,S.A. and Sward,K.
  TITLE     Myocardin regulates exon usage in smooth muscle cells through
            induction of splicing regulatory factors
  JOURNAL   Cell Mol Life Sci 79 (8), 459 (2022)
   PUBMED   35913515
  REMARK    GeneRIF: Myocardin regulates exon usage in smooth muscle cells
            through induction of splicing regulatory factors.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8565)
  AUTHORS   Mikhailov,A.T. and Torrado,M.
  TITLE     In search of novel targets for heart disease: myocardin and
            myocardin-related transcriptional cofactors
  JOURNAL   Biochem Res Int 2012, 973723 (2012)
   PUBMED   22666593
REFERENCE   6  (bases 1 to 8565)
  AUTHORS   Du,K.L., Chen,M., Li,J., Lepore,J.J., Mericko,P. and Parmacek,M.S.
  TITLE     Megakaryoblastic leukemia factor-1 transduces cytoskeletal signals
            and induces smooth muscle cell differentiation from
            undifferentiated embryonic stem cells
  JOURNAL   J Biol Chem 279 (17), 17578-17586 (2004)
   PUBMED   14970199
REFERENCE   7  (bases 1 to 8565)
  AUTHORS   Wang,Z., Wang,D.Z., Pipes,G.C. and Olson,E.N.
  TITLE     Myocardin is a master regulator of smooth muscle gene expression
  JOURNAL   Proc Natl Acad Sci U S A 100 (12), 7129-7134 (2003)
   PUBMED   12756293
REFERENCE   8  (bases 1 to 8565)
  AUTHORS   Du,K.L., Ip,H.S., Li,J., Chen,M., Dandre,F., Yu,W., Lu,M.M.,
            Owens,G.K. and Parmacek,M.S.
  TITLE     Myocardin is a critical serum response factor cofactor in the
            transcriptional program regulating smooth muscle cell
            differentiation
  JOURNAL   Mol Cell Biol 23 (7), 2425-2437 (2003)
   PUBMED   12640126
  REMARK    GeneRIF: Myocardin is a critical serum response factor cofactor
            regulating smooth muscle cell differentiation; forced expression of
            myocardin activated expression of SM22 alpha, smooth muscle
            alpha-actin, and calponin-h1 genes in mouse ES cells
REFERENCE   9  (bases 1 to 8565)
  AUTHORS   Wang,D.Z., Li,S., Hockemeyer,D., Sutherland,L., Wang,Z.,
            Schratt,G., Richardson,J.A., Nordheim,A. and Olson,E.N.
  TITLE     Potentiation of serum response factor activity by a family of
            myocardin-related transcription factors
  JOURNAL   Proc Natl Acad Sci U S A 99 (23), 14855-14860 (2002)
   PUBMED   12397177
REFERENCE   10 (bases 1 to 8565)
  AUTHORS   Wang,D., Chang,P.S., Wang,Z., Sutherland,L., Richardson,J.A.,
            Small,E., Krieg,P.A. and Olson,E.N.
  TITLE     Activation of cardiac gene expression by myocardin, a
            transcriptional cofactor for serum response factor
  JOURNAL   Cell 105 (7), 851-862 (2001)
   PUBMED   11439182
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005358.1 and AK292885.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 24, 2020 this sequence version replaced NM_001146312.2.
            
            Summary: This gene encodes a nuclear protein, which is expressed in
            heart, aorta, and in smooth muscle cell-containing tissues. It
            functions as a transcriptional co-activator of serum response
            factor (SRF) and modulates expression of cardiac and smooth
            muscle-specific SRF-target genes, and thus may play a crucial role
            in cardiogenesis and differentiation of the smooth muscle cell
            lineage. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene.[provided by
            RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.1286588.1,
                                           SRR14372079.1683722.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000425538.6/ ENSP00000401678.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-236               AC005358.1         74204-74439
            237-3574            AK292885.1         106-3443
            3575-8565           AC005358.1         172273-177263
FEATURES             Location/Qualifiers
     source          1..8565
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p12"
     gene            1..8565
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /note="myocardin"
                     /db_xref="GeneID:93649"
                     /db_xref="HGNC:HGNC:16067"
                     /db_xref="MIM:606127"
     exon            1..354
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     CDS             300..3260
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="myocardin isoform 1"
                     /protein_id="NP_001139784.1"
                     /db_xref="CCDS:CCDS54091.1"
                     /db_xref="GeneID:93649"
                     /db_xref="HGNC:HGNC:16067"
                     /db_xref="MIM:606127"
                     /translation="MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKR
                     PAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRA
                     RLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSD
                     GLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQS
                     DAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAE
                     KSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNP
                     NSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSG
                     TKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFG
                     STSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPS
                     ELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFL
                     AASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVL
                     SSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQV
                     CTAQNSGAHDGHPPSFSPHSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKMAGLHS
                     SDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEM
                     PADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLE
                     VLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQT
                     QFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTD
                     LNLNSSMDLHLQQW"
     exon            355..420
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            421..476
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            477..552
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            553..714
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            715..890
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1016
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1270
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1424
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..2357
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2501
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2630
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            2631..2688
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     exon            2689..8565
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6928..6933
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /note="hexamer: AATAAA"
     polyA_site      6954
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
     regulatory      8540..8545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /note="hexamer: AATAAA"
     polyA_site      8565
                     /gene="MYOCD"
                     /gene_synonym="MGBL; MYCD"
                     /note="major polyA site"
ORIGIN      
        1 aatcgccggc agcctatgac atcagacagg aacgcctggg atgccgcgct gctcctggcc
       61 aacctccgag gaggaggagg gtcccgccgg ctaagagtta attagccccg cacggcgagg
      121 ggggaggcgc cagttttctg gggacactgg ctgccactgt actcctaccc aggggagctc
      181 acggagagtt ggatgaattc tgggttgtta gctgcggtca gctgggctcc cgggagcctg
      241 ttgctggtgg agaacagggg gcgcctggcc aagggaccag cggcttgctg agactcaaca
      301 tgacactcct ggggtctgag cattccttgc tgattaggag caagttcaga tcagttttac
      361 agttaagact tcaacaaaga aggacccagg aacaactggc taaccaaggc ataataccac
      421 cactgaaacg tccagctgaa ttccatgagc aaagaaaaca tttggatagt gacaaggcta
      481 aaaattccct gaagcgcaaa gccagaaaca ggtgcaacag tgccgacttg gttaatatgc
      541 acatactcca agcttccact gcagagaggt ccattccaac tgctcagatg aagctgaaaa
      601 gagcccgact cgccgatgat ctcaatgaaa aaattgctct acgaccaggg ccactggagc
      661 tggtggaaaa aaacattctt cctgtggatt ctgctgtgaa agaggccata aaaggtaacc
      721 aggtgagttt ctccaaatcc acggatgctt ttgcctttga agaggacagc agcagcgatg
      781 ggctttctcc ggatcagact cgaagtgaag acccccaaaa ctcagcggga tccccgccag
      841 acgctaaagc ctcagatacc ccttcgacag gttctctggg gacaaaccag gatcttgctt
      901 ctggctcaga aaatgacaga aatgactcag cctcacagcc cagccaccag tcagatgcgg
      961 ggaagcaggg gcttggcccc cccagcaccc ccatagccgt gcatgctgct gtaaagtcca
     1021 aatccttggg tgacagtaag aaccgccaca aaaagcccaa ggaccccaag ccaaaggtga
     1081 agaagcttaa atatcaccag tacattcccc cagaccagaa ggcagagaag tcccctccac
     1141 ctatggactc agcctacgct cggctgctcc agcaacagca gctgttcctg cagctccaaa
     1201 tcctcagcca gcagcagcag cagcagcaac accgattcag ctacctaggg atgcaccaag
     1261 ctcagcttaa ggaaccaaat gaacagatgg tcagaaatcc aaactcttct tcaacgccac
     1321 tgagcaatac ccccttgtct cctgtcaaaa acagtttttc tggacaaact ggtgtctctt
     1381 ctttcaaacc aggcccactc ccacctaacc tggatgatct gaaggtctct gaattaagac
     1441 aacagcttcg aattcggggc ttgcctgtgt caggcaccaa aacggctctc atggaccggc
     1501 ttcgaccctt ccaggactgc tctggcaacc cagtgccgaa ctttggggat ataacgactg
     1561 tcacttttcc tgtcacaccc aacacgctgc ccaattacca gtcttcctct tctaccagtg
     1621 ccctgtccaa cggcttctac cactttggca gcaccagctc cagccccccg atctccccag
     1681 cctcctctga cctgtcagtc gctgggtccc tgccggacac cttcaatgat gcctccccct
     1741 ccttcggcct gcacccgtcc ccagtccacg tgtgcacgga ggaaagtctc atgagcagcc
     1801 tgaatggggg ctctgttcct tctgagctgg atgggctgga ctccgagaag gacaagatgc
     1861 tggtggagaa gcagaaggtg atcaatgaac tcacctggaa actccagcaa gagcagaggc
     1921 aggtggagga gctgaggatg cagcttcaga agcagaaaag gaataactgt tcagagaaga
     1981 agccgctgcc tttcctggct gcctccatca agcaggaaga ggctgtctcc agctgtcctt
     2041 ttgcatccca agtacctgtg aaaagacaaa gcagcagctc agagtgtcac ccaccggctt
     2101 gtgaagctgc tcaactccag cctcttggaa atgctcattg tgtggagtcc tcagatcaaa
     2161 ccaatgtact ttcttccaca tttctcagcc cccagtgttc ccctcagcat tcaccgctgg
     2221 gggctgtgaa aagcccacag cacatcagtt tgcccccatc acccaacaac cctcactttc
     2281 tgccctcatc ctccggggcc cagggagaag ggcacagggt ctcctcgccc atcagcagcc
     2341 aggtgtgcac tgcacagaac tcaggagcac acgatggcca tcctccaagc ttctctcccc
     2401 attcttccag cctccacccg cccttctctg gagcccaagc agacagcagt catggtgccg
     2461 ggggaaaccc ttgtcccaaa agcccatgtg tacagcaaaa gatggctggt ttacactctt
     2521 ctgataaggt ggggccaaag ttttcaattc catccccaac tttttctaag tcaagttcag
     2581 caatttcaga ggtaacacag cctccatcct atgaagatgc cgtaaagcag caaatgaccc
     2641 ggagtcagca gatggatgaa ctcctggacg tgcttattga aagcggagaa atgccagcag
     2701 acgctagaga ggatcactca tgtcttcaaa aagtcccaaa gatacccaga tcttcccgaa
     2761 gtccaactgc tgtcctcacc aagccctcgg cttcctttga acaagcctct tcaggcagcc
     2821 agatcccctt tgatccctat gccaccgaca gtgatgagca tcttgaagtc ttattaaatt
     2881 cccagagccc cctaggaaag atgagtgatg tcacccttct aaaaattggg agcgaagagc
     2941 ctcactttga tgggataatg gatggattct ctgggaaggc tgcagaagac ctcttcaatg
     3001 cacatgagat cttgccaggc cccctctctc caatgcagac acagttttca ccctcttctg
     3061 tggacagcaa tgggctgcag ttaagcttca ctgaatctcc ctgggaaacc atggagtggc
     3121 tggacctcac tccgccaaat tccacaccag gctttagcgc cctcaccacc agcagcccca
     3181 gcatcttcaa catcgatttc ctggatgtca ctgatctcaa tttgaattct tccatggacc
     3241 ttcacttgca gcagtggtag aatgcccaat gcaccagtgc tatggaagac caatggagtt
     3301 ccatggggga aagcacacag ccatacatac tttactgtcc aaaaacagaa gaagaagaag
     3361 agaattaaaa agaagcaatg atttctgtgc caatgaacaa gaacaaaagt catttttaga
     3421 aatacatata ctgtaatatt taccaacagt cagtaactgt taatgatttc aacaatgcat
     3481 taaaagaatg tgctttctca gattaaggat gccaaaaaag atatttcact gccttttcaa
     3541 agaccagtat attttctagc ccataatttt tctcaggcat tgttggggca taagctcaca
     3601 ctgtaagctt ttctcatgaa ttcactagac ataacgtgga aggaaaacgt agtcttttgg
     3661 gagtacaggg aagccagccc ctcaaagctt atggaagaca tacctgcaat ggaagctgtt
     3721 gcccaatgtc tccattacta tctttcaaaa gagaagccag acccagcttc agatcaaaag
     3781 ttcttgagac agaggaacaa aaccaatcga tttccaggga agctaatcaa ctctcttttc
     3841 cctctaccac aaaactgccc tgctggagtg gttctgaacc tgtacccagg actcgatgtg
     3901 gtcactaata acaattaacc tgaactgagt ccacagaact ccactcggaa ctttcttctt
     3961 ttttaactag tggcccaatc attcccacca tctctgtgct gataagtacg tgtcctagat
     4021 gagaaccctg aagaatgcag accttcttcc cccgaaggag atgccacaag ctctccaaca
     4081 cagccccctt tagttccaaa gactagagat gaccacattg gtagaagtat atctcgaggc
     4141 acaggaaggg agccccacca gggataattc agacaggact agagaataac atcatttcac
     4201 ataccctggg ataaacaccc tgggttccta tagaaggact attacttatg ggagtccaac
     4261 ttctcctttt gttttgttat tatcagttta tctttctccc actccacttt tccttcaagg
     4321 taccaatcct ttcctgttcc tcgtttggcc atctttcttt ttctgcctcc acattgggag
     4381 gggaggactt ctcagttcta acaagctgcc atactcctaa gaaagccatt tttgaaaaat
     4441 ttaacaatcc aggttcttct ggagaactca ttctccacac gcacagtttg ctgcaaaagg
     4501 aagttgcaag aatttcttga ggaagaaact ggtgacttgg tccatcagtc acgaagttct
     4561 ttctattctc gtttagtttt caagaaatta ttggtttgtg ttgctctggg gaaattggaa
     4621 atcattacat tgtaaagaca aatatggatg atatttacaa gagagaattt cagatctggg
     4681 tttttgaaag aaaacagaat tgcgcattga aaacgatgga aggaaaaaga caatggtcta
     4741 atgtgcattc ctcattacct ctcgtggctt tggctgggag ttggaaaaag ctaaaatttc
     4801 agaacagtct ctgtaaggct ctctgtggct ccagttcacc attttatatt gttgcatgct
     4861 gtagaaagga gctattgctg ttgttttgtt tttttattta aatcactaag gcactgtttt
     4921 tatcttttgt aaaaaaaaaa aaaaagttgt tcactgtgca cttatagaaa aaataatcaa
     4981 aaatgttggg attttagaag ctctcttttt gataaaccaa agatttagaa gtcattccat
     5041 tgttaacttg taaaaatgtg tgaacacaga gagtttttgg tgattgctac tctgaaagct
     5101 gccagatctt attctggggg tgggatgtgg aggaatacac atacacacac aaacatacat
     5161 gtatgtataa tagatatata catatgtgta tattatatct gtgtgtgcat gtatctccaa
     5221 aagcggcgtt acagagttct acaccaaaag cctttaaccc ttaatctgct gtgaatgata
     5281 cctggccttt ctcactatga atttctgatt aaccaaccag actacacgtt gcctctctgt
     5341 gtatgactaa cggctccaac ccgatgactc acagctactt gcttatcgtg aacaagctca
     5401 tcttggcaat gaatatggat gtgaaaagac agaacagctt caccattagt agctggaaat
     5461 ggtatcacag tctcttatag aggaatatga aaggaacaag aaaatcattt tacattcctt
     5521 ttatctgtat tgtgctttaa aagatccaca tggtaaattt tttattttgc ttttatgtca
     5581 gtcatcagaa ccaaaaaaat ccagaagaaa aaattgccag tgtttccttt gaagatgaag
     5641 ctactgggga agaaaacctt attaatacac tccacacatt tgttcattcc tcagctgttg
     5701 gtgttttctt ggggtcttga caaagcttgc tggtcagtgc acttttcagg tgtcacgttt
     5761 tgctgtttgt atgttttttc ttccccttac ttcctttgga aaacaaactc acacagtgcc
     5821 cctactctga gacctgggac tgagtgttaa ttattttttc cttgggtatt tctatctgag
     5881 agactagacc tagttaggag gcctctgtac ttctccagat tgtacctttt tatggggatc
     5941 tttgaggcta tgacccagga ctgatagata tgccttacgg aagacaaaag ataaaatggt
     6001 tcctatatcc taatgcaaac caacacagtt aaaagagcag atctctggat aactgctctc
     6061 aacctgcttc tacagtctcc acaaaccgca ttcaccctct ctcttcatag ctcagacatg
     6121 aaatttgagg gagaaaactg gagataattg ggagaaaatt gatgaagttg gctgcttcca
     6181 gtagatcaga taatccatga atttgtctcc cattgagaat tttattttaa attcttttaa
     6241 actcttcgtt gtgtcttttg tgatgacaaa tcaggcatga ctaaaagatg tacagagact
     6301 tacgaagatg gtcacattca agttccctaa tgctcttaga acctgaagat gaccatgtgt
     6361 agttttctta agacctctga acccccatgg tgatgaagac ttgaagacat ttgcagctat
     6421 ctgctgcagt ctggtagatt catacttatc taaagaagtc aaaaaattta ttcgtgcaag
     6481 tgcttgcagg aagccagtgc ttattagtag tgaccctgct tctatcaacg ttattgagac
     6541 aacacatatt ctattctaag ggagaaagag ggaggaagag agggagggag ggaggaagaa
     6601 gagggaggga gcgaggaagg aagataggag atgggtaggg gggtaaagag aaaaggaggg
     6661 agaagggaag gaaggaaaga agagaggaaa gaaaggaggg aaggaaaaaa gggccaaact
     6721 ttctgatcta tgaacttctc agttcagctg tcacattatg agaagtaaat cagaattttt
     6781 ttaaggagaa gtcattctta gcactacaat aattgtacca gtaattgagg aaaccaagac
     6841 aatcttcacc tgaataatag agggtctgag aactgtcagc cttttgccat tcaaaaacat
     6901 ttatgtccaa cctgaaaaaa aagcatcaat aaaacctatc ccaagcattc aaaatagtcc
     6961 tttccaaatg ttatttattt taaagtcaat cagctctttt agaaacagat tctggtctgg
     7021 ctgaaaactc ccacaacaaa tttactcatc cagtggctaa tatttaatgc ccaccatggg
     7081 cagagcacac aaatcttcag ataaacaata ctgaattgat tcagcacaga ttgttcagat
     7141 ttgaatgacc agggagttgt atttgcacat gcaagaacac taagaatctc ccagtcctca
     7201 aactagaaac ctttcagcta cgatgaaaaa aaaaaagggg tcttcatttt tccaagaggg
     7261 ggtggaggtg gggatcactt tttagctaaa agctatctct cacttcaaaa ttcttgtctt
     7321 tttctttgtg gacaaacacc agtagtctat cacttggaga tcttttaata tctcccatca
     7381 tttaaaacat ccacgagagt ttgaagattt gtgttgattg ccagatacag aagccccttg
     7441 aaaataagga aagggtggag gaagcatttt tgtgtcctat ccctacttat cgtagcagct
     7501 ctatagacaa aagggacact tactggtgag cctctggccc ttaaaagaaa atcatctaag
     7561 aatatgaagg caatttgatt tccccccaca gccctcagct gccttcctca cagaaggaag
     7621 ttcccaaaat tgctggtaca cagtttgcaa tcaaatatca gatatgagaa aacctgtagt
     7681 gaagagtctg ggttcttggt tttctcataa atccaatata aatttgtagg ttggttcagg
     7741 gtcaaaattg ccagtgcttt attagacaga tgatactgat agacacacag agcccaggtc
     7801 ctggaacaag acaatcctgt agtgccaaga tctggtcagt tgcgttaagg agctgggttt
     7861 gattctagag tccaggttta tagagaaacc ctggctagat tgagcctacc catggggaga
     7921 cgatttcaag acaggatgag atctgggaag aattttgttg tcatctgcca gggaaattat
     7981 cacaggactc attgaatgca ataacatgtg agtaagttcc cttttgattc tgggaatcag
     8041 cgattttccc tgtggattaa gacaaaccaa cgccagaagg tctcctgtgc ttattttaac
     8101 catctgctcc catcgtgaac cctggagcat gcatttccta gaagtggttt catagctcct
     8161 gtgtgttcat ggaaaagggg agtataatga tggggatgct ggaagctttt ttaatgtttt
     8221 ccaaaggaaa ggaacccaca ctgctcccca gagttccttt ccaatggccc tgcagtaaga
     8281 acggaggaca atgtattgct gggtgcttaa aatcctccct cagtgaagca caaagagaca
     8341 ctttgtaaag aaaaaaagag caagcatagg ttctctgtgg gaccttgtgg agtggtgttt
     8401 tcacgttggt ctctttggct caattgagca taatcagaaa gaaatgtggg ttattgggaa
     8461 gagacaaaaa gcagtggcta aaataccaaa gttggcatgt gttctttttt aaaaaaaaaa
     8521 aaaaatgcat atatttttaa ataaaatgtt tattttaaaa agaaa
//
