LOCUS       NM_004999               8615 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens myosin VI (MYO6), transcript variant 1, mRNA.
ACCESSION   NM_004999 XM_376516
VERSION     NM_004999.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Zheng,S., Hong,Z., Tan,Y., Wang,Y., Li,J., Zhang,Z., Feng,T.,
            Hong,Z., Lin,G. and Ye,D.
  TITLE     MYO6 contributes to tumor progression and enzalutamide resistance
            in castration-resistant prostate cancer by activating the focal
            adhesion signaling pathway
  JOURNAL   Cell Commun Signal 22 (1), 517 (2024)
   PUBMED   39449086
  REMARK    GeneRIF: MYO6 contributes to tumor progression and enzalutamide
            resistance in castration-resistant prostate cancer by activating
            the focal adhesion signaling pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Raghuvanshi,R., Panda,K.C., Ray,C.S. and Ramchander,P.V.
  TITLE     Targeted Next-Generation Sequencing Analysis Reveals a Novel
            Genetic Variant in MYO6 Gene in an Indian Family with Postlingual
            Nonsyndromic Hearing Loss
  JOURNAL   Genet Test Mol Biomarkers 28 (8), 328-336 (2024)
   PUBMED   39019031
  REMARK    GeneRIF: Targeted Next-Generation Sequencing Analysis Reveals a
            Novel Genetic Variant in MYO6 Gene in an Indian Family with
            Postlingual Nonsyndromic Hearing Loss.
REFERENCE   3  (bases 1 to 8615)
  AUTHORS   Canon,L., Kikuti,C., Planelles-Herrero,V.J., Lin,T., Mayeux,F.,
            Sirkia,H., Lee,Y.I., Heidsieck,L., Velikovsky,L., David,A., Liu,X.,
            Moussaoui,D., Forest,E., Hook,P., Petersen,K.J., Morgan,T.E., Di
            Cicco,A., Sires-Campos,J., Derivery,E., Levy,D., Delevoye,C.,
            Sweeney,H.L. and Houdusse,A.
  TITLE     How myosin VI traps its off-state, is activated and dimerizes
  JOURNAL   Nat Commun 14 (1), 6732 (2023)
   PUBMED   37872146
  REMARK    GeneRIF: How myosin VI traps its off-state, is activated and
            dimerizes.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8615)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 8615)
  AUTHORS   Yang,A., Liu,X., Liu,P., Feng,Y., Liu,H., Gao,S., Huo,L., Han,X.,
            Wang,J. and Kong,W.
  TITLE     LncRNA UCA1 promotes development of gastric cancer via the
            miR-145/MYO6 axis
  JOURNAL   Cell Mol Biol Lett 26 (1), 33 (2021)
   PUBMED   34238213
  REMARK    GeneRIF: LncRNA UCA1 promotes development of gastric cancer via the
            miR-145/MYO6 axis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8615)
  AUTHORS   Avraham,K.B., Hasson,T., Sobe,T., Balsara,B., Testa,J.R.,
            Skvorak,A.B., Morton,C.C., Copeland,N.G. and Jenkins,N.A.
  TITLE     Characterization of unconventional MYO6, the human homologue of the
            gene responsible for deafness in Snell's waltzer mice
  JOURNAL   Hum Mol Genet 6 (8), 1225-1231 (1997)
   PUBMED   9259267
REFERENCE   7  (bases 1 to 8615)
  AUTHORS   Avraham,K.B., Hasson,T., Steel,K.P., Kingsley,D.M., Russell,L.B.,
            Mooseker,M.S., Copeland,N.G. and Jenkins,N.A.
  TITLE     The mouse Snell's waltzer deafness gene encodes an unconventional
            myosin required for structural integrity of inner ear hair cells
  JOURNAL   Nat Genet 11 (4), 369-375 (1995)
   PUBMED   7493015
REFERENCE   8  (bases 1 to 8615)
  AUTHORS   Hasson,T. and Mooseker,M.S.
  TITLE     Porcine myosin-VI: characterization of a new mammalian
            unconventional myosin
  JOURNAL   J Cell Biol 127 (2), 425-440 (1994)
   PUBMED   7929586
REFERENCE   9  (bases 1 to 8615)
  AUTHORS   Bement,W.M., Hasson,T., Wirth,J.A., Cheney,R.E. and Mooseker,M.S.
  TITLE     Identification and overlapping expression of multiple
            unconventional myosin genes in vertebrate cell types
  JOURNAL   Proc Natl Acad Sci U S A 91 (14), 6549-6553 (1994)
   PUBMED   8022818
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11767. doi:
            10.1073/pnas.91.24.11767-c.. PMID: 7972138]
REFERENCE   10 (bases 1 to 8615)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB088907.1, AB002387.1,
            AL136093.8 and AL392166.19.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_004999.3.
            
            Summary: This gene encodes a reverse-direction motor protein that
            moves toward the minus end of actin filaments and plays a role in
            intracellular vesicle and organelle transport. The protein consists
            of a motor domain containing an ATP- and an actin-binding site and
            a globular tail which interacts with other proteins. This protein
            maintains the structural integrity of inner ear hair cells and
            mutations in this gene cause non-syndromic autosomal dominant and
            recessive hearing loss. Alternative splicing results in multiple
            transcript variants encoding distinct isoforms. [provided by
            RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes isoform (1). Isoforms
            1 and 8 are the same length but differ in sequence.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.189978.1,
                                           SRR1660807.103545.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2144335, SAMEA2155770
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369977.8/ ENSP00000358994.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-93                DB088907.1         64-156
            94-4099             AB002387.1         1-4006
            4100-7846           AL136093.8         81449-85195
            7847-8615           AL392166.19        102-870
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q14.1"
     gene            1..8615
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="myosin VI"
                     /db_xref="GeneID:4646"
                     /db_xref="HGNC:HGNC:7605"
                     /db_xref="MIM:600970"
     exon            1..185
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            186..349
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     CDS             233..4090
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="isoform 1 is encoded by transcript variant 1;
                     unconventional myosin-6; unconventional myosin-VI"
                     /codon_start=1
                     /product="unconventional myosin-VI isoform 1"
                     /protein_id="NP_004990.3"
                     /db_xref="CCDS:CCDS34487.1"
                     /db_xref="GeneID:4646"
                     /db_xref="HGNC:HGNC:7605"
                     /db_xref="MIM:600970"
                     /translation="MEDGKPVWAPHPTDGFQMGNIVDIGPDSLTIEPLNQKGKTFLAL
                     INQVFPAEEDSKKDVEDNCSLMYLNEATLLHNIKVRYSKDRIYTYVANILIAVNPYFD
                     IPKIYSSEAIKSYQGKSLGTRPPHVFAIADKAFRDMKVLKMSQSIIVSGESGAGKTEN
                     TKFVLRYLTESYGTGQDIDDRIVEANPLLEAFGNAKTVRNNNSSRFGKFVEIHFNEKS
                     SVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGASEDIREKLHLSSPDNFRYLNR
                     GCTRYFANKETDKQILQNRKSPEYLKAGSMKDPLLDDHGDFIRMCTAMKKIGLDDEEK
                     LDLFRVVAGVLHLGNIDFEEAGSTSGGCNLKNKSAQSLEYCAELLGLDQDDLRVSLTT
                     RVMLTTAGGTKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSY
                     FIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILKEEQELYQKEGLGVNEVHY
                     VDNQDCIDLIEAKLVGILDILDEENRLPQPSDQHFTSAVHQKHKDHFRLTIPRKSKLA
                     VHRNIRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRELFESS
                     TNNNKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRSTGASFIRCIKPNLKMTSHHFEG
                     AQILSQLQCSGMVSVLDLMQGGYPSRASFHELYNMYKKYMPDKLARLDPRLFCKALFK
                     ALGLNENDYKFGLTKVFFRPGKFAEFDQIMKSDPDHLAELVKRVNHWLTCSRWKKVQW
                     CSLSVIKLKNKIKYRAEACIKMQKTIRMWLCKRRHKPRIDGLVKVGTLKKRLDKFNEV
                     VSVLKDGKPEMNKQIKNLEISIDTLMAKIKSTMMTQEQIQKEYDALVKSSEELLSALQ
                     KKKQQEEEAERLRRIQEEMEKERKRREEDEKRRRKEEEERRMKLEMEAKRKQEEEERK
                     KREDDEKRIQAEVEAQLARQKEEESQQQAVLEQERRDRELALRIAQSEAELISDEAQA
                     DLALRRNDGTRPKMTPEQMAKEMSEFLSRGPAVLATKAAAGTKKYDLSKWKYAELRDT
                     INTSCDIELLAACREEFHRRLKVYHAWKSKNKKRNTETEQRAPKSVTDYDFAPFLNNS
                     PQQNPAAQIPARQREIEMNRQQRFFRIPFIRPADQYKDPQSKKKGWWYAHFDGPWIAR
                     QMELHPDKPPILLVAGKDDMEMCELNLEETGLTRKRGAEILPRQFEEIWERCGGIQYL
                     QNAIESRQARPTYATAMLQSLLK"
     exon            350..419
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            420..493
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            494..623
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            624..729
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            730..785
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            786..883
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1048
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1129
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1310
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1455
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1613
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1705
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1778
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1906
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2002
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2176
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2215
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2309
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2440
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2518
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2519..2648
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2649..2739
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2890
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            2891..3099
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..3178
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3179..3339
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3340..3369
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3370..3408
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3409..3512
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3513..3644
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3645..3671
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3890
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     exon            3891..8615
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5290..5295
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="hexamer: AATAAA"
     polyA_site      5309
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="major polyA site"
     regulatory      5665..5670
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="hexamer: AGTAAA"
     polyA_site      5691
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="major polyA site"
     regulatory      5764..5769
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="hexamer: AGTAAA"
     polyA_site      5783
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
     regulatory      8590..8595
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
                     /note="hexamer: AATAAA"
     polyA_site      8615
                     /gene="MYO6"
                     /gene_synonym="DFNA22; DFNB37"
ORIGIN      
        1 ggagaccgac tcgggatctg tccgagcagg aagccagcct cagcccggcc gctgtcgccg
       61 ccctgtcctg gtgcccgtcc gcgtcgtcgc cctcttcact ggccctcatc acttctcacc
      121 gcgccctcca gcttcacccg tacaggtagc cccgccgccg cgcacctgcc ttcgctcccg
      181 caccggtgac agtggatagt ggaaacagga gatcgtggat cctccttcaa aaatggagga
      241 tggaaagccc gtttgggcgc cacaccctac agatggattt cagatgggca atattgtgga
      301 tattggcccc gacagcttaa caattgaacc cttgaatcag aaaggcaaga catttttggc
      361 tctcataaac caagtgtttc ctgcagaaga ggacagtaaa aaagatgtgg aagataactg
      421 ttcactaatg tatttaaatg aagccacact gctccataat atcaaagttc gatatagtaa
      481 agacagaatt tatacatatg tcgccaacat tctgattgca gtgaatccat actttgacat
      541 acctaaaata tattcttcag aagcaataaa gtcatatcaa ggaaaatctc ttgggacaag
      601 accacctcat gtctttgcaa ttgctgataa agcttttcga gacatgaagg tgctcaagat
      661 gagtcagtct atcattgtat ctggagaatc aggagccggc aaaacagaaa atacaaaatt
      721 tgttctaaga tacctgactg aatcctatgg aacaggtcaa gatattgatg acagaattgt
      781 tgaagctaac ccactcctag aagcctttgg aaatgcgaag actgttcgca acaataatag
      841 cagtcgattt gggaaatttg tagaaataca ttttaatgaa aagagctcag ttgttggagg
      901 atttgtttca cattatctcc tagagaaatc taggatctgt gttcaaggca aagaggaaag
      961 aaattatcat atcttttata ggttgtgtgc tggtgcttct gaagatatta gagaaaaact
     1021 tcatttgagt tcaccagata attttcggta tttaaaccga ggctgcacta gatactttgc
     1081 taacaaagaa actgacaaac agattttaca gaaccgcaaa agtcctgagt accttaaggc
     1141 aggttctatg aaagatcctc tgctagatga ccatggtgat tttattagaa tgtgcacggc
     1201 tatgaaaaaa attggtttgg atgatgaaga aaagcttgat ctcttccggg tagtagctgg
     1261 cgtcctgcac cttggaaata ttgattttga ggaagctggc agcacttcag gtggttgtaa
     1321 tctgaagaat aaatctgctc agtctttgga atattgtgct gaattactgg gtttggacca
     1381 agatgatctt cgagtaagtt tgaccacaag agtcatgcta acaacagcag ggggcaccaa
     1441 aggaacagtt ataaaggtac ctctgaaagt ggagcaagca aacaatgctc gtgatgccct
     1501 ggcaaagaca gtgtatagcc atctttttga tcatgtggta aacagagtaa atcagtgttt
     1561 tccttttgaa acatcatcct attttattgg agtcctagat attgctggtt ttgagtactt
     1621 tgagcataac agttttgaac aattttgcat caactattgc aatgaaaaac ttcaacaatt
     1681 ttttaatgaa aggattctga aggaggaaca agaactctat caaaaagaag gtttaggtgt
     1741 taatgaagtg cattatgtgg ataatcagga ctgtatagat ttaattgaag ccaaattagt
     1801 gggaatactg gatattttgg atgaagaaaa tcgccttccc cagccaagtg atcaacactt
     1861 tacatctgca gttcaccaaa agcacaagga tcattttcga ctcactattc ccagaaaatc
     1921 taagctggca gttcatagga atatcagaga cgacgaaggc ttcattatca ggcattttgc
     1981 gggggcagtg tgctatgaaa caacccagtt tgtggagaaa aataatgatg ctttacatat
     2041 gtctcttgaa tccttaatat gtgaatccag agataagttt atacgggaat tatttgaatc
     2101 atccacaaat aacaacaaag atactaaaca aaaagcagga aaacttagct tcatcagcgt
     2161 gggaaacaag tttaagacac agttaaattt gcttctggat aaacttcgaa gtactggagc
     2221 aagctttatt cgttgcatca aacctaactt aaagatgaca agccaccact ttgaaggtgc
     2281 tcaaattctg tctcagcttc agtgttcagg gatggtgtct gttttggact tgatgcaggg
     2341 tggttaccca tcacgagctt catttcatga actctacaac atgtacaaaa agtatatgcc
     2401 agataaactt gcaagattgg atccaagact attttgtaag gctttgttta aagctttggg
     2461 cttaaatgaa aatgactaca agtttgggtt aaccaaagta ttttttagac ctggcaagtt
     2521 tgcagaattt gatcagatca tgaagtctga ccctgaccac ttagcagagt tggttaaaag
     2581 agtcaatcac tggctcacat gcagtcgctg gaagaaagtt cagtggtgct cactctcagt
     2641 catcaaattg aaaaacaaaa taaaatatcg agctgaagcc tgcattaaaa tgcaaaaaac
     2701 tattcgaatg tggctttgca agaggagaca caaacctcgc attgatggtc tggttaaggt
     2761 gggcacactg aaaaaacgac ttgataaatt taatgaggta gtcagtgtgt tgaaagatgg
     2821 aaaacccgag atgaataaac agatcaagaa tctggaaatt tctattgata ctttgatggc
     2881 caaaattaag tccactatga tgacgcagga acaaatccag aaagaatatg atgcactggt
     2941 taaaagctca gaggaactcc tcagtgcatt acagaaaaaa aaacagcagg aagaggaagc
     3001 agaaaggctg aggcgtattc aagaagaaat ggaaaaggaa agaaaaagac gtgaagaaga
     3061 cgaaaaacgt cgaagaaagg aagaggagga aaggcggatg aaacttgaga tggaagcaaa
     3121 gagaaaacaa gaagaagaag agagaaagaa aagggaagat gatgaaaaac gcattcaagc
     3181 tgaagtggag gcacagctgg cccgacagaa ggaggaggaa tcccaacagc aagcagttct
     3241 ggagcaggag cgcagggacc gggagctggc cctgaggatt gcccagagtg aagccgagct
     3301 catcagtgat gaggcccagg ccgacctggc gctgcggaga aatgatggaa caagacccaa
     3361 aatgacaccg gaacaaatgg ccaaagaaat gtcagaattt ttgagtagag gtcctgctgt
     3421 actagccacc aaagcagctg ctggtactaa gaaatatgat cttagtaaat ggaaatatgc
     3481 agaactacgt gataccatca atacttcttg tgatattgag ctcctggcag cttgcagaga
     3541 agaatttcat aggagactaa aagtgtatca tgcttggaaa tctaagaaca agaagagaaa
     3601 tactgaaaca gagcaacgtg ctccaaagtc tgttactgat tatgattttg caccattttt
     3661 gaacaattca cctcagcaaa acccagcagc tcagattcct gccaggcagc gggagattga
     3721 aatgaaccga cagcaacgct tcttccgcat cccattcatc cgccctgccg accagtacaa
     3781 agaccctcag agtaagaaaa aaggctggtg gtatgcccat tttgatggac catggattgc
     3841 ccggcaaatg gaactccatc ctgacaagcc acccatccta cttgtggctg gtaaggacga
     3901 catggagatg tgtgagctga atcttgagga gactggcctg actcggaagc gtggtgctga
     3961 gatcttgcca agacagtttg aagaaatctg ggaacgctgt ggaggcatcc agtaccttca
     4021 gaatgcgatt gagagcagac aggctcggcc cacctatgca acagccatgc tgcagagtct
     4081 gttaaagtag atgttgcaca ccagccttac agctgggagc ctttgccatg gtacttaggt
     4141 agggtgtgtg cccccagatt taaccattcc ataatcatgt tagagttact tctataaagt
     4201 gaacagattt tattaatcac ggcttttggt gaatttgttt aaggttaatt atggtagcaa
     4261 attttggacc taaacattat ttttctgtat cccgctgtaa ttcccaaaac tctcattatt
     4321 ctctaactat tacacatggg catattctga tgtttctcat cctttgccag aagactacct
     4381 tacatccatc gtaattgttc tctaggaaaa gagaactttt ttcaaaattc aaaatacttt
     4441 ttaaggatgg cacagtacca tataactgga gtaataaaac atgagcttac attcttacaa
     4501 taactaaacc acttaaaatg atcaaggcac taatgttttg gtctgaaaag ctgtgtactt
     4561 tatagacatt ttcagacatt tttggaaatt tccattaaag gtggaaaatc tatttttttc
     4621 ctcctttgca gtgtcttagt ttgaatgaaa cacttcgaag ttctagaatt ctagaaagag
     4681 ccttaatgta tttgatgtat tctgtgataa gaggtactaa tagtatccag cacagatttg
     4741 cttttctttg ctagcacaat gtgtgttgct gtcagaatat tctttttata ttctgtggaa
     4801 aaataaagga aattcagatt gtttaaatgc ctaaaagttt tgagataagt tttgtttcaa
     4861 ttagaaaagg aaataggttt taggtggcat agtggcttaa ctggactgaa ttcaaatatt
     4921 ctttcaactt catctcaata gtgatttttg tatcagaatc ttgtccaagt tgtttcattg
     4981 atttagtaag tgttctgctt ccaacatctt tctttttaag aaattcctag tgtctttttt
     5041 ggcctttgag gttttggtaa ttgtagacct gtttcataag ctttgtaatt cagaaatcct
     5101 tgtatttagt aagtgcttgt tttacataac tgataatttt aaaatgtttt ctttgtgtgc
     5161 tgttagtatt gattcaaatg tcagcagctt taagcctaat atttatgact ttcacatttg
     5221 gaatttaaag acaaaaatac atcaaggagt tatgctgaca taattctaag gagttttgtt
     5281 gtattttaga ataaaattat aaagtaaaat gattctctgt actgcttttt cccccagttt
     5341 ttagagaccc taacctttga aatgaaattc cagtgatttc ttttttccct agaaagatta
     5401 cctcagttag ggaagtattt cccagctgac tagtgtttgt gagccacaga cactgtcttc
     5461 agaattgctt ctctcatgtc ttagtagaga aatatttatt tattatgata cattcaaatg
     5521 attgtcaagt taaattaaat ggttgtgtct gtgctattga gaatgcaaat gtgattatct
     5581 tttgaaggct gtattactgc atagcttcac ccaccctcgg gtcatttcgt ccctgtgatt
     5641 ggggacagaa ggtgtagcta ctgaagtaaa tgacctattc tctctcttcc atctctcgcc
     5701 tttaactggt gtttttattt gtgtaggata gtgaatgata agcttttttc ctaaccagta
     5761 gtgagtaaag ttcttgaaca aaatttagta gccaaattgt tttttaatga catgtctctt
     5821 tagtacaata gttttgtgta tcttttagat acattaatag gcactagatg gaaaattaaa
     5881 gagttaaaca tatttaaatg agagaatcta atgtttcaga aatttgtaag aaatgtatca
     5941 cagcaaaggg ttgttataag tccttagttt ttgactctaa tagttaatac aattatagtt
     6001 aatcttaagc cataatgttt ctaatcatgt cacacagctg tcctagaact tatctattta
     6061 aaatagtttc ctgagttaat tttggccagc agggcaactg ccctaattca gatagattta
     6121 cagtaaccta cgtacagtag atgcacatac acacagacac ccctttgctg gagaaactta
     6181 ggaccctgtc agccttttaa aggaaacagc aggagtggtg tcctaaatga tgttcatgca
     6241 gctgctttac catgttcaca gtcaagccca tgcatgccag gttaaaactg tggaaatcaa
     6301 aagtaaattc actcatattt taatcatttt aactgagatt taaaattaga agtttaaacc
     6361 actatatata aagaactaat cttttcttaa taccagttct ttccatagca tatgctttgc
     6421 aaaggcagca tgcataaaat atttaaaatg agaggacaga atgttttcac atttgattca
     6481 attttaatat aattcctaat tgtggtaaca cagttgagat atgtattatg agttatggga
     6541 actaattgag aaaaggaagt tactctaatc cacgtatgtt aagagaatat tgagttttct
     6601 tagttgtaaa gttggggaga tggcaccttc tcagaggatt gtgaaaatat gaggaagaaa
     6661 caaaacagtg catgtaggag cacagggcca cacaaaggca ttctattgtt atgctcattc
     6721 tgcttctgta atgacttttc ataggtcatt cttgtgaacc attttgtttt gcaagcaacc
     6781 aaggaaagaa catcttaagt ggaaaatcag tggtggttgt gaacacttag agaatagcaa
     6841 tccacaggca agaataatgg tattgtttgt agagctttat taattggata ttttttaaaa
     6901 gacattttca ttcacaggtc attactatgg ttctcagcga tccaaatatg tagatcattg
     6961 gttttttttt ttacctgaag tagcttaaga gtacttggat cagtagaata aatatttatt
     7021 gaatcaatca gtcagccaat taatatgatg ttagtgatag acctgcctcc ttttatggaa
     7081 gaggtaacag atccagagag gtcaagtaat ttagttgtag actgaaaaat atatcaaagc
     7141 ctttgctgca atcatatgta acaaaaagaa ccaaaacaaa cactttttag tggcacctgt
     7201 ggatttacaa agggttgcct ctctgtcatt ccacaacttc agaaggtgtg acaggttttc
     7261 cctatttatc attaccaata ataacaagta ttgagagttt taaaatttct cccagaagat
     7321 aaactaacaa ggatggaagg ggagggcaaa ggatatctaa acatgagaat aaggacatgt
     7381 tagagggggg gaaacagttg taacaataag gaaagagaag agcaacagtg gaagagacag
     7441 gttgtgtgcc cctaaagatt ctgcaccccc agtttggaaa cactgataca ttttaggaca
     7501 cagagcactc ctagatctct acgaaatttt agaatgaata atgtgtaatt tataggatca
     7561 gaacgtatgg ttattaaaac ttggatcaag atatgcccgg tgtatacatt cttagcacat
     7621 aggaatggca ctgccatact ggagaaggtc agcagtaaat aggcattctg tacataagcc
     7681 tcatggaagg gtaagatgga gagactggca gaagtagcac ctactctgct gggagcactt
     7741 ctctgagtac gctttagttc aattcaaatc actgtattct ttccccattg ctaacctaat
     7801 atatgaaaca agcttagctg tctcagaagt ttttcaagag atgatcagga aaaattaatg
     7861 cacattcaaa aggagaatct tcagtacaaa tttgtttttt taaaaataga tttagggctg
     7921 ggcgcggtgg ctcacgcctg taatcccagc actttgggag gccgaggcgg gtggatcacg
     7981 aggtcaagac ttcgagacca gcctggccaa catggtgaaa ccctgtctct actaaaaata
     8041 caaaacatta gccaggcgtg gtagtgggtg cctgtaatcc ctgctacttg ggaggctgag
     8101 gcaggagaat cacttgaacg cgggaggcag aggttgcagg gagccgagat ggcgccattg
     8161 cactccatcc tgggcaacaa gagcgaaatt ccatctcaaa aaataaaata gatttagggg
     8221 gtacaagtgc agttttgtta catgggtata ttgcatagtg gtaaaatgtg ggcttttagt
     8281 gtacctaaca cccagagaag catacattgt gcccagtagg taatttttca tccctaaacc
     8341 ttttctcagc ctcccacttt ctggagtctc caatgtcggt tattccactc tttatgtcca
     8401 tatctacaca ttcaatccta atttgtacca agtagcatct cacctttaaa tcacaggctt
     8461 attagttggg tgttttcttt ttacttatga aaattcatct agtcaaactg tcaattaatt
     8521 tttcctcatt tcattaaaag tgtatatcta atgctttctc taaaaattga tgtactggaa
     8581 atacaaataa ataaatgctc cctgtgtaga atttc
//
