LOCUS       NM_018728               6977 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens myosin VC (MYO5C), mRNA.
ACCESSION   NM_018728
VERSION     NM_018728.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Holthenrich,A., Terglane,J., Nass,J., Mietkowska,M., Kerkhoff,E.
            and Gerke,V.
  TITLE     Spire1 and Myosin Vc promote Ca2+-evoked externalization of von
            Willebrand factor in endothelial cells
  JOURNAL   Cell Mol Life Sci 79 (2), 96 (2022)
   PUBMED   35084586
  REMARK    GeneRIF: Spire1 and Myosin Vc promote Ca(2+)-evoked externalization
            of von Willebrand factor in endothelial cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Tesolin,P., Fiorino,S., Lenarduzzi,S., Rubinato,E., Cattaruzzi,E.,
            Ammar,L., Castro,V., Orzan,E., Granata,C., Dell'Orco,D., Morgan,A.
            and Girotto,G.
  TITLE     Pendred Syndrome, or Not Pendred Syndrome? That Is the Question
  JOURNAL   Genes (Basel) 12 (10), 1569 (2021)
   PUBMED   34680964
  REMARK    GeneRIF: Pendred Syndrome, or Not Pendred Syndrome? That Is the
            Question.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6977)
  AUTHORS   Dolce,L.G., Ohbayashi,N., Silva,D.F.C.D., Ferrari,A.J.R.,
            Pirolla,R.A.S., Schwarzer,A.C.A.P., Zanphorlin,L.M., Cabral,L.,
            Fioramonte,M., Ramos,C.H.I., Gozzo,F.C., Fukuda,M., Giuseppe,P.O.
            and Murakami,M.T.
  TITLE     Unveiling the interaction between the molecular motor Myosin Vc and
            the small GTPase Rab3A
  JOURNAL   J Proteomics 212, 103549 (2020)
   PUBMED   31698103
  REMARK    GeneRIF: Unveiling the interaction between the molecular motor
            Myosin Vc and the small GTPase Rab3A.
REFERENCE   4  (bases 1 to 6977)
  AUTHORS   Sladewski,T.E., Krementsova,E.B. and Trybus,K.M.
  TITLE     Myosin Vc Is Specialized for Transport on a Secretory Superhighway
  JOURNAL   Curr Biol 26 (16), 2202-2207 (2016)
   PUBMED   27498562
  REMARK    GeneRIF: MyoVc is unique in taking variable size steps, which are
            frequently in the backward direction. Results obtained with
            chimeric constructs implicate the lever arm/rod of MyoVc as being
            responsible for these properties. Actin bundles allow single MyoVc
            motors to move processively.
REFERENCE   5  (bases 1 to 6977)
  AUTHORS   Wulf,S.F., Ropars,V., Fujita-Becker,S., Oster,M., Hofhaus,G.,
            Trabuco,L.G., Pylypenko,O., Sweeney,H.L., Houdusse,A.M. and
            Schroder,R.R.
  TITLE     Force-producing ADP state of myosin bound to actin
  JOURNAL   Proc Natl Acad Sci U S A 113 (13), E1844-E1852 (2016)
   PUBMED   26976594
  REMARK    GeneRIF: The myosin V structure reveals the detailed rearrangements
            underlying myosin force generation as well as the basis of
            strain-dependent ADP release that is essential for processive
            myosins, such as myosin V.
REFERENCE   6  (bases 1 to 6977)
  AUTHORS   Roland,J.T., Lapierre,L.A. and Goldenring,J.R.
  TITLE     Alternative splicing in class V myosins determines association with
            Rab10
  JOURNAL   J Biol Chem 284 (2), 1213-1223 (2009)
   PUBMED   19008234
  REMARK    GeneRIF: related Rab protein, Rab10, can interact with myosin Va,
            myosin Vb, and myosin Vc
REFERENCE   7  (bases 1 to 6977)
  AUTHORS   Watanabe,S., Watanabe,T.M., Sato,O., Awata,J., Homma,K., Umeki,N.,
            Higuchi,H., Ikebe,R. and Ikebe,M.
  TITLE     Human myosin Vc is a low duty ratio nonprocessive motor
  JOURNAL   J Biol Chem 283 (16), 10581-10592 (2008)
   PUBMED   18079121
  REMARK    GeneRIF: myosin Vc is a low duty ratio nonprocessive motor
REFERENCE   8  (bases 1 to 6977)
  AUTHORS   Takagi,Y., Yang,Y., Fujiwara,I., Jacobs,D., Cheney,R.E.,
            Sellers,J.R. and Kovacs,M.
  TITLE     Human myosin Vc is a low duty ratio, nonprocessive molecular motor
  JOURNAL   J Biol Chem 283 (13), 8527-8537 (2008)
   PUBMED   18201966
  REMARK    GeneRIF: Kinetic simulations reveal that the high ADP affinity of
            actomyosin Vc may lead to elevations of the duty ratio of myosin Vc
            to as high as 64% under possible physiological ADP concentrations
REFERENCE   9  (bases 1 to 6977)
  AUTHORS   Rodriguez,O.C. and Cheney,R.E.
  TITLE     Human myosin-Vc is a novel class V myosin expressed in epithelial
            cells
  JOURNAL   J Cell Sci 115 (Pt 5), 991-1004 (2002)
   PUBMED   11870218
REFERENCE   10 (bases 1 to 6977)
  AUTHORS   Bement,W.M., Hasson,T., Wirth,J.A., Cheney,R.E. and Mooseker,M.S.
  TITLE     Identification and overlapping expression of multiple
            unconventional myosin genes in vertebrate cell types
  JOURNAL   Proc Natl Acad Sci U S A 91 (14), 6549-6553 (1994)
   PUBMED   8022818
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11767. doi:
            10.1073/pnas.91.24.11767-c.. PMID: 7972138]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC010674.11.
            
            On Nov 23, 2018 this sequence version replaced NM_018728.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF272390.1, SRR7346977.2792627.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261839.12/ ENSP00000261839.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-195               AC010674.11        165833-166027       c
            196-306             AC010674.11        153005-153115       c
            307-472             AC010674.11        149732-149897       c
            473-617             AC010674.11        149096-149240       c
            618-774             AC010674.11        145785-145941       c
            775-918             AC010674.11        142803-142946       c
            919-1000            AC010674.11        141986-142067       c
            1001-1108           AC010674.11        139976-140083       c
            1109-1215           AC010674.11        134413-134519       c
            1216-1481           AC010674.11        131085-131350       c
            1482-1563           AC010674.11        126862-126943       c
            1564-1704           AC010674.11        123540-123680       c
            1705-1830           AC010674.11        121613-121738       c
            1831-1914           AC010674.11        118923-119006       c
            1915-2049           AC010674.11        117681-117815       c
            2050-2147           AC010674.11        117140-117237       c
            2148-2234           AC010674.11        116179-116265       c
            2235-2346           AC010674.11        115577-115688       c
            2347-2558           AC010674.11        114579-114790       c
            2559-2724           AC010674.11        112271-112436       c
            2725-2871           AC010674.11        109956-110102       c
            2872-3036           AC010674.11        107705-107869       c
            3037-3130           AC010674.11        105893-105986       c
            3131-3194           AC010674.11        102845-102908       c
            3195-3375           AC010674.11        99356-99536         c
            3376-3469           AC010674.11        95662-95755         c
            3470-3533           AC010674.11        95298-95361         c
            3534-3614           AC010674.11        95124-95204         c
            3615-3795           AC010674.11        93767-93947         c
            3796-3889           AC010674.11        91385-91478         c
            3890-3953           AC010674.11        89982-90045         c
            3954-4122           AC010674.11        88742-88910         c
            4123-4210           AC010674.11        84826-84913         c
            4211-4309           AC010674.11        83411-83509         c
            4310-4464           AC010674.11        81953-82107         c
            4465-4554           AC010674.11        78777-78866         c
            4555-4705           AC010674.11        76039-76189         c
            4706-4988           AC010674.11        75088-75370         c
            4989-5163           AC010674.11        66532-66706         c
            5164-5244           AC010674.11        65600-65680         c
            5245-6977           AC010674.11        62545-64277         c
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.2"
     gene            1..6977
                     /gene="MYO5C"
                     /note="myosin VC"
                     /db_xref="GeneID:55930"
                     /db_xref="HGNC:HGNC:7604"
                     /db_xref="MIM:610022"
     exon            1..195
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     CDS             169..5397
                     /gene="MYO5C"
                     /note="myosin 5C; myosin-Vc"
                     /codon_start=1
                     /product="unconventional myosin-Vc"
                     /protein_id="NP_061198.2"
                     /db_xref="CCDS:CCDS42036.1"
                     /db_xref="GeneID:55930"
                     /db_xref="HGNC:HGNC:7604"
                     /db_xref="MIM:610022"
                     /translation="MAVAELYTQYNRVWIPDPEEVWKSAEIAKDYRVGDKVLRLLLED
                     GTELDYSVNPESLPPLRNPDILVGENDLTALSYLHEPAVLHNLRIRFAESKLIYTYSG
                     IILVAMNPYKQLPIYGDAIIHAYSGQNMGDMDPHIFAVAEEAYKQMARNNRNQSIIVS
                     GESGAGKTVSARYAMRYFATVSKSGSNAHVEDKVLASNPITEAVGNAKTTRNDNSSRF
                     GKYTEISFDEQNQIIGANMSTYLLEKSRVVFQSENERNYHIFYQLCASAQQSEFKHLK
                     LGSAEEFNYTRMGGNTVIEGVNDRAEMVETQKTFTLLGFKEDFQMDVFKILAAILHLG
                     NVQITAVGNERSSVSEDDSHLKVFCELLGLESGRVAQWLCNRKIVTSSETVVKPMTRP
                     QAVNARDALAKKIYAHLFDFIVERINQALQFSGKQHTFIGVLDIYGFETFDVNSFEQF
                     CINYANEKLQQQFNMHVFKLEQEEYMKEDIPWTLIDFYDNQPVIDLIEAKMGILELLD
                     EECLLPHGTDENWLQKLYNNFVNRNPLFEKPRMSNTSFVIQHFADKVEYKCEGFLEKN
                     RDTVYDMLVEILRASKFHLCANFFQENPTPPSPFGSMITVKSAKQVIKPNSKHFRTTV
                     GSKFRSSLYLLMETLNATTPHYVRCIKPNDEKLPFEFDSKRIVQQLRACGVLETIRIS
                     AQSYPSRWTYIEFYSRYGILMTKQELSFSDKKEVCKVVLHRLIQDSNQYQFGKTKIFF
                     RAGQVAYLEKLRLDKLRQSCVMVQKHMRGWLQRKKFLRERRAALIIQQYFRGQQTVRK
                     AITAVALKEAWAAIIIQKHCRGYLVRSLYQLIRMATITMQAYSRGFLARRRYRKMLEE
                     HKAVILQKYARAWLARRRFQSIRRFVLNIQLTYRVQRLQKKLEDQNKENHGLVEKLTS
                     LAALRAGDVEKIQKLEAELEKAATHRRNYEEKGKRYRDAVEEKLAKLQKHNSELETQK
                     EQIQLKLQEKTEELKEKMDNLTKQLFDDVQKEERQRMLLEKSFELKTQDYEKQIQSLK
                     EEIKALKDEKMQLQHLVEGEHVTSDGLKAEVARLSKQVKTISEFEKEIELLQAQKIDV
                     EKHVQSQKREMREKMSEITKQLLESYDIEDVRSRLSVEDLEHLNEDGELWFAYEGLKK
                     ATRVLESHFQSQKDCYEKEIEALNFKVVHLSQEINHLQKLFREENDINESIRHEVTRL
                     TSENMMIPDFKQQISELEKQKQDLEIRLNEQAEKMKGKLEELSNQLHRSQEEEGTQRK
                     ALEAQNEIHTKEKEKLIDKIQEMQEASDHLKKQFETESEVKCNFRQEASRLTLENRDL
                     EEELDMKDRVIKKLQDQVKTLSKTIGKANDVHSSSGPKEYLGMLQYKREDEAKLIQNL
                     ILDLKPRGVVVNMIPGLPAHILFMCVRYADSLNDANMLKSLMNSTINGIKQVVKEHLE
                     DFEMLSFWLSNTCHFLNCLKQYSGEEEFMKHNSPQQNKNCLNNFDLSEYRQILSDVAI
                     RIYHQFIIIMEKNIQPIIVPGMLEYESLQGISGLKPTGFRKRSSSIDDTDGYTMTSVL
                     QQLSYFYTTMCQNGLDPELVRQAVKQLFFLIGAVTLNSLFLRKDMCSCRKGMQIRCNI
                     SYLEEWLKDKNLQNSLAKETLEPLSQAAWLLQVKKTTDSDAKEIYERCTSLSAVQIIK
                     ILNSYTPIDDFEKRVTPSFVRKVQALLNSREDSSQLMLDTKYLFQVTFPFTPSPHALE
                     MIQIPSSFKLGFLNRL"
     exon            196..306
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            307..472
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            473..617
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            618..774
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            775..918
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1000
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1108
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1215
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1481
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..1563
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1704
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1830
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..1914
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2049
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2147
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2234
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            2235..2346
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2558
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2724
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2871
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            2872..3036
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3037..3130
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3194
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3195..3375
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3469
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3470..3533
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3614
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3615..3795
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3796..3889
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3890..3953
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..4122
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            4123..4210
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            4211..4309
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            4310..4464
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            4465..4554
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            4555..4705
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            4706..4988
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            4989..5163
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            5164..5244
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     exon            5245..6977
                     /gene="MYO5C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6927..6932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO5C"
                     /note="hexamer: AATGAA"
     regulatory      6959..6964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO5C"
                     /note="hexamer: AATAAA"
     polyA_site      6965
                     /gene="MYO5C"
     polyA_site      6977
                     /gene="MYO5C"
                     /note="major polyA site"
ORIGIN      
        1 gccgccaact cgggaacggg aacaggaggc cgttggaaaa ctttgggcaa gatggcccgg
       61 cggtgatttc cgcggcggtc tctcctgcgc ccggcctctg cggcgcaggc ccagcccccg
      121 agcctcgcac gttcggcagc cccggcctgg ccccggcccc tcctgcccat ggcggtggcc
      181 gagctgtaca cgcagtacaa cagggtctgg attcccgatc ctgaagaagt ttggaagtct
      241 gctgaaatag ccaaggacta cagagttggt gacaaggtcc tgcgactcct gctggaggat
      301 ggaacggagc tggattattc tgtcaatcca gaatctctgc ctccacttcg gaatcctgac
      361 atcctcgtgg gcgagaatga cctcacggct ctcagctatc ttcacgagcc cgcggtgctc
      421 cacaacctca gaatccgctt tgcagaatcc aaactcattt acacctacag tggaatcatt
      481 ttggtggcca tgaatcctta caagcagttg ccaatatacg gagatgccat catccacgcc
      541 tacagcgggc agaacatggg cgatatggac ccacacatat ttgccgtggc agaagaggca
      601 tacaagcaga tggccagaaa caacagaaac cagtccataa ttgtaagtgg ggagtcaggt
      661 gctggaaaga cagtgtcggc tcgctatgcc atgaggtact ttgccaccgt cagcaaatcg
      721 ggcagcaacg ctcacgtgga agacaaggtc ctggcatcca atcccatcac cgaggccgtt
      781 ggaaatgcca agaccacccg caatgacaat agtagtcggt ttgggaaata cacagaaatc
      841 agttttgatg aacaaaatca aattatagga gccaacatga gcacttacct cctggagaaa
      901 tccagagttg tctttcaatc ggaaaatgaa cgaaattacc acattttcta tcagctttgt
      961 gcatctgcac agcagtcgga atttaaacat cttaaattgg ggagtgccga agaatttaat
     1021 tatacaagaa tgggaggcaa tactgtcatt gagggtgtga atgatcgagc tgaaatggta
     1081 gagactcaaa agaccttcac gcttctgggt ttcaaggagg attttcagat ggacgttttt
     1141 aaaatcctgg cagccatcct acatctgggc aatgtgcaga tcaccgcggt gggcaacgag
     1201 aggtcctcag ttagtgagga tgacagtcac ctgaaggtgt tctgtgagct cctgggcctg
     1261 gagagtggca gagttgctca gtggctgtgc aatcgcaaaa tcgtcacaag ctctgagacg
     1321 gtggtaaaac ccatgaccag gcctcaggct gtcaacgcca gggatgcact ggccaaaaag
     1381 atctatgctc acctgttcga cttcattgtg gagagaatta accaagcgtt gcagttttca
     1441 ggcaagcagc acacttttat tggtgttttg gacatttatg gttttgaaac ctttgatgtg
     1501 aacagctttg aacaattttg catcaattac gctaatgaaa aactgcaaca acagtttaac
     1561 atgcatgtct tcaaactgga acaagaagaa tacatgaagg aagatatacc ttggacgctg
     1621 atagattttt atgacaatca accagttatt gacctgattg aagcaaaaat gggaattctg
     1681 gagttactgg atgaagaatg tttgttacca catggaactg atgaaaactg gcttcaaaag
     1741 ctgtataata attttgtcaa caggaaccct ttgtttgaaa agcctagaat gtcaaacaca
     1801 tcctttgtca tccagcactt tgctgataag gtagagtata aatgtgaagg tttcctggag
     1861 aaaaacagag acaccgtcta tgacatgctg gttgaaatcc tgagagcaag caagtttcat
     1921 ctctgtgcca acttttttca agaaaatcca actcctcctt ctccttttgg ttcaatgatt
     1981 acagttaaat ctgcaaagca agtcatcaag ccaaacagca agcatttccg gaccacagtt
     2041 gggagcaagt tccgcagctc tctgtacttg ctcatggaga ccctcaatgc gacgacgccc
     2101 cactacgttc gatgcatcaa gccaaatgat gagaagttac cctttgaatt tgactccaaa
     2161 agaattgttc agcagctgcg agcctgcggc gttttagaaa cgattcgcat tagtgcacag
     2221 agctaccctt ccaggtggac atacatcgag ttctacagtc gctacggcat tctcatgacc
     2281 aagcaagagc tttccttcag cgataaaaag gaggtgtgca aggtggtttt acacagactc
     2341 atccaggatt ctaatcagta ccagtttggt aaaaccaaaa ttttcttcag agcaggacaa
     2401 gtggcttatt tagagaaact tcgattggat aaactgaggc agagttgtgt tatggtacaa
     2461 aagcacatgc gtggctggct ccagaggaaa aaattcctcc gagagagacg agccgccctg
     2521 ataatccagc agtacttccg gggtcagcaa actgtgagga aagctattac tgcagtggcc
     2581 ttaaaagaag cttgggcagc cataatcatt cagaagcact gccgcgggta tcttgttcgc
     2641 agcctgtatc agttgattcg catggccacc atcacaatgc aggcctacag ccgaggattc
     2701 ctggcaagga ggaggtatcg aaagatgctg gaggaacata aggctgtgat cctacagaaa
     2761 tacgcacggg cgtggctggc cagacgcaga ttccagagta tccgacgatt cgtgcttaat
     2821 attcagctta cttacagggt ccagcgtttg cagaaaaagt tggaagatca gaacaaagaa
     2881 aaccatgggc tggtggagaa gctgactagc ctggctgctc ttcgagctgg ggatgtggaa
     2941 aagattcaga agctggaagc agaactagaa aaagcagcca ctcacaggcg aaattacgag
     3001 gagaagggga agagatacag ggatgctgtg gaagagaaat tggcaaagct tcagaagcat
     3061 aattcagaac tggaaacaca gaaagaacaa atacagctga agcttcaaga gaagactgaa
     3121 gagttaaaag aaaaaatgga caacctcacc aagcagctct ttgatgatgt acaaaaggaa
     3181 gaacggcaaa gaatgcttct tgaaaaaagt tttgaactga aaacacaaga ctatgagaag
     3241 cagattcagt ctttgaaaga agaaattaaa gctctcaagg atgagaagat gcaactccaa
     3301 cacctggtgg agggggagca cgtcacttct gatggcttga aggcggaagt ggcccgcctg
     3361 agcaagcagg tcaagacaat ctctgagttc gaaaaagaga tagaactact tcaggcacag
     3421 aagatagatg tggagaaaca tgtgcagtca caaaaacggg aaatgagaga aaagatgtca
     3481 gagatcacca aacaacttct cgaaagctat gacattgaag atgtaagaag caggctctct
     3541 gtggaagatc tggaacattt aaatgaggat ggagaacttt ggtttgctta tgaaggacta
     3601 aagaaagcaa cacgtgtttt ggagagccat ttccagtctc agaaggattg ctatgaaaag
     3661 gagattgaag ctttgaactt caaagtggtg catctcagtc aagaaatcaa ccacctgcag
     3721 aagttattca gagaagaaaa tgacatcaat gaaagcatcc gtcatgaagt taccaggcta
     3781 acatcagaga acatgatgat cccagacttt aaacagcaaa tttcagaatt ggagaaacag
     3841 aagcaagatc ttgaaatccg cctgaatgaa caagctgaga aaatgaaagg aaaactagaa
     3901 gaattgtcta atcagttaca tcgcagtcaa gaggaggaag gaacacaaag aaaggccttg
     3961 gaagcccaaa atgaaataca taccaaagag aaggagaagc tgattgataa gattcaagaa
     4021 atgcaggagg ccagtgacca cttgaagaaa caatttgaaa ctgaaagtga agtcaagtgt
     4081 aacttccggc aggaagcatc ccggctcact ttggaaaaca gggatcttga agaagaatta
     4141 gacatgaaag acagagtgat taaaaagcta caagatcaag tcaagacact aagcaagaca
     4201 attggaaaag ccaatgatgt gcactcgtcc tcaggaccca aggagtacct tggaatgctg
     4261 caatacaaga gagaagacga ggccaagctc attcagaacc tcattcttga cttgaagccc
     4321 cgtggcgtgg tggtgaacat gatccccggg ctgccggctc atatcctgtt catgtgtgtg
     4381 cgctacgcag actctctgaa tgatgccaac atgctgaagt ccctcatgaa cagcaccatt
     4441 aatggcatca agcaggtggt taaggaacat ttagaagact ttgaaatgct gtccttttgg
     4501 ctttccaaca cttgtcattt tctcaattgc ctgaagcagt acagcggaga agaggaattc
     4561 atgaagcata atagtccaca gcagaataag aattgcttga acaattttga cctttcagaa
     4621 tacagacaga ttctcagtga tgtggctata cgaatatatc atcaatttat tatcataatg
     4681 gaaaagaata tccaaccgat aatagttccg ggaatgctgg agtatgagag cctgcagggc
     4741 atttccggcc tgaagcccac aggcttccgg aagcgctcct ctagcataga cgacacggac
     4801 ggctacacca tgacctccgt cctgcaacag ctgagctact tttacaccac catgtgccag
     4861 aacggcctgg accccgagct tgtgaggcag gcggtgaagc agctcttctt cttgatcggg
     4921 gcggtcacgc tgaacagcct cttcctgcgc aaggacatgt gctcctgcag aaaagggatg
     4981 cagatcaggt gcaatatcag ctacttagaa gaatggctta aagataagaa cttgcagaac
     5041 agcttagcaa aggaaacttt ggagcccctc tctcaggcag cctggttgct tcaggtcaag
     5101 aagaccacag acagtgatgc caaggagatc tacgaacgct gcacctcact gtctgctgtg
     5161 cagatcataa agatccttaa ttcatacaca cctatagatg actttgagaa gagagtgact
     5221 ccatcctttg ttcgcaaagt acaggctctc ctaaatagcc gggaggattc atcacagctg
     5281 atgttggata ccaaatatct ctttcaagtc acatttcctt ttaccccctc tccacatgct
     5341 ctggaaatga ttcagatccc cagcagtttc aagctaggct ttctgaatag gttatagcag
     5401 gagaaaaagt caatgcactt tttcttcaaa agctaagtga aggttcaata tgagggatgt
     5461 gttttattaa ttggcagtgg aaattggaat gtgatttaag gaagaaaaca gacttttctc
     5521 aaatggacca catcatctct cactcacagt gacaatgctg ctcctgtaat tttgtaagaa
     5581 ttcacacaca ccagctgtat ttggaaaagc catgtctcac tctcgaatct cagctggttt
     5641 tcactgaaat tcctggaggg ctcatatttt cttccacgtg cctcttgatc tgttctgtct
     5701 tgtggcaggg ctctaccagg agggagtgag atctggggac acagtggctt ccaagttttc
     5761 taaggttcat gtgaaacctg agtacattta caaagctgca agtggattct agtgctgagt
     5821 cttccaggaa aaaatcccgt ttggccaccg tgacctgcca aaagttctct tgccccatgc
     5881 ctgctgggga cagaaggttt tcatgaactc tttttactga aaatgccctg catttaaaaa
     5941 tgtttatttt agacccttct aaattttttt tttttttttt ttttgagact ccggagtctc
     6001 gctctatcgc caggctggag tgtagtggca tgatctcggc ttattgcaac ctccgcttcc
     6061 tgggttcaaa caattctcct gcctcagcct cctgagtagc tgggactaca ggcacctgcc
     6121 accacgccca gctaattttt gtatttttag tggagacagg tttcaccatg ttggccagga
     6181 tggcctcgat ctcttgacct cgtgatccgc ccacctcagc ctcccaaagt gctaggatta
     6241 caggcgtgtg ccaccgcgcc cggctgaccc ttttaatttt taagagtgtt caatccgaga
     6301 tgaatcattt gaagtatttt tatatgtata tctatttaaa actaatatat tattaaagct
     6361 taattgccat gccgtttatc ttctctgaaa gaacttcaaa tcttacctgc caacatattc
     6421 accataattt ttttattttt taataccttt catacaataa cttttttaaa acaacctcag
     6481 attgaaaaag caacctaaat tactttcgct ctctaatcag catttcaatg tatttatttt
     6541 taaattttct caaaaagtaa ctaaaaaatt gtgtcggacc ctacttttga gaaatctacg
     6601 tttcccaagt tttatgggaa ctggctattc cttgtcccgg cacaccttct cattccttcc
     6661 tttcagagcc taaaacctca tttgataagc actcctagtc tctggcctgt ggatccagtg
     6721 ctattctgtc accaacctaa gaatcccaat tgcaccttct gtttctgaca gtcacaggtg
     6781 acagctgtga ttctataata cagactggtg tcttagaggt aggaataata catgattatg
     6841 aagcatcacc ctgctaatac ataataatgt ctttttatat tataagtgat tgagtttagt
     6901 tcatttcaat acattgtaca tgaaaaaatg aaaagtagaa ctttgtaata ctttaatcaa
     6961 taaaattaat taccaaa
//
