LOCUS       NM_017433               5624 bp    mRNA    linear   PRI 11-DEC-2024
DEFINITION  Homo sapiens myosin IIIA (MYO3A), transcript variant 1, mRNA.
ACCESSION   NM_017433
VERSION     NM_017433.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Cirilo,J.A. Jr., Liao,X., Perrin,B.J. and Yengo,C.M.
  TITLE     The dynamics of actin protrusions can be controlled by
            tip-localized myosin motors
  JOURNAL   J Biol Chem 300 (1), 105516 (2024)
   PUBMED   38042485
  REMARK    GeneRIF: The dynamics of actin protrusions can be controlled by
            tip-localized myosin motors.
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Gunther,L.K., Cirilo,J.A. Jr., Desetty,R. and Yengo,C.M.
  TITLE     Deafness mutation in the MYO3A motor domain impairs actin
            protrusion elongation mechanism
  JOURNAL   Mol Biol Cell 33 (1), ar5 (2022)
   PUBMED   34788109
  REMARK    GeneRIF: Deafness mutation in the MYO3A motor domain impairs actin
            protrusion elongation mechanism.
REFERENCE   3  (bases 1 to 5624)
  AUTHORS   Bueno,A.S., Nunes,K., Dias,A.M.M., Alves,L.U., Mendes,B.C.A.,
            Sampaio-Silva,J., Smits,J., Yntema,H.G., Meyer,D., Lezirovitz,K.
            and Mingroni-Netto,R.C.
  TITLE     Frequency and origin of the c.2090T>G p.(Leu697Trp) MYO3A variant
            associated with autosomal dominant hearing loss
  JOURNAL   Eur J Hum Genet 30 (1), 13-21 (2022)
   PUBMED   33953343
  REMARK    GeneRIF: Frequency and origin of the c.2090T>G p.(Leu697Trp) MYO3A
            variant associated with autosomal dominant hearing loss.
REFERENCE   4  (bases 1 to 5624)
  AUTHORS   Souissi,A., Abdelmalek Driss,D., Chakchouk,I., Ben Said,M., Ben
            Ayed,I., Mosrati,M.A., Elloumi,I., Tlili,A., Aifa,S. and
            Masmoudi,S.
  TITLE     Molecular insights into MYO3A kinase domain variants explain
            variability in both severity and progression of DFNB30 hearing
            impairment
  JOURNAL   J Biomol Struct Dyn 40 (21), 10940-10951 (2022)
   PUBMED   34423747
  REMARK    GeneRIF: Molecular insights into MYO3A kinase domain variants
            explain variability in both severity and progression of DFNB30
            hearing impairment.
REFERENCE   5  (bases 1 to 5624)
  AUTHORS   Wonkam,A., Manyisa,N., Bope,C.D., Dandara,C. and Chimusa,E.R.
  TITLE     Whole exome sequencing reveals pathogenic variants in MYO3A, MYO15A
            and COL9A3 and differential frequencies in ancestral alleles in
            hearing impairment genes among individuals from Cameroon
  JOURNAL   Hum Mol Genet 29 (23), 3729-3743 (2021)
   PUBMED   33078831
  REMARK    GeneRIF: Whole exome sequencing reveals pathogenic variants in
            MYO3A, MYO15A and COL9A3 and differential frequencies in ancestral
            alleles in hearing impairment genes among individuals from
            Cameroon.
REFERENCE   6  (bases 1 to 5624)
  AUTHORS   Komaba,S., Inoue,A., Maruta,S., Hosoya,H. and Ikebe,M.
  TITLE     Determination of human myosin III as a motor protein having a
            protein kinase activity
  JOURNAL   J Biol Chem 278 (24), 21352-21360 (2003)
   PUBMED   12672820
  REMARK    GeneRIF: class III myosin is an actin-based motor protein having a
            protein kinase activity
REFERENCE   7  (bases 1 to 5624)
  AUTHORS   Walsh,T., Walsh,V., Vreugde,S., Hertzano,R., Shahin,H., Haika,S.,
            Lee,M.K., Kanaan,M., King,M.C. and Avraham,K.B.
  TITLE     From flies' eyes to our ears: mutations in a human class III myosin
            cause progressive nonsyndromic hearing loss DFNB30
  JOURNAL   Proc Natl Acad Sci U S A 99 (11), 7518-7523 (2002)
   PUBMED   12032315
  REMARK    GeneRIF: mutation in humans causes progressive nonsyndromic hearing
            loss DFNB30
REFERENCE   8  (bases 1 to 5624)
  AUTHORS   Dose,A.C. and Burnside,B.
  TITLE     Cloning and chromosomal localization of a human class III myosin
  JOURNAL   Genomics 67 (3), 333-342 (2000)
   PUBMED   10936054
REFERENCE   9  (bases 1 to 5624)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
REFERENCE   10 (bases 1 to 5624)
  AUTHORS   Wallace,H. and Maden,M.
  TITLE     Irradiation inhibits the regeneration of aneurogenic limbs
  JOURNAL   J Exp Zool 195 (3), 353-358 (1976)
   PUBMED   1262820
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD628208.1, AY101367.1,
            CD628204.1, AL391812.7, AL360217.14, AL162503.12 and CK903348.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_017433.4.
            
            Summary: The protein encoded by this gene belongs to the myosin
            superfamily. Myosins are actin-dependent motor proteins and are
            categorized into conventional myosins (class II) and unconventional
            myosins (classes I and III through XV) based on their variable
            C-terminal cargo-binding domains. Class III myosins, such as this
            one, have a kinase domain N-terminal to the conserved N-terminal
            motor domains and are expressed in photoreceptors. The protein
            encoded by this gene plays an important role in hearing in humans.
            Three different recessive, loss of function mutations in the
            encoded protein have been shown to cause nonsyndromic progressive
            hearing loss. Expression of this gene is highly restricted, with
            the strongest expression in retina and cochlea. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY101367.1, AF229172.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000642920.2/ ENSP00000495965.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                CD628208.1         166-206
            42-1457             AY101367.1         16-1431
            1458-1757           CD628204.1         285-584
            1758-2109           AY101367.1         1732-2083
            2110-2318           AL391812.7         60750-60958
            2319-2466           AL360217.14        4529-4676
            2467-3498           AY101367.1         2441-3472
            3499-3602           AL360217.14        46574-46677
            3603-4497           AL162503.12        3629-4523
            4498-5619           AY101367.1         4472-5593
            5620-5624           CK903348.1         19-23               c
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p12.1"
     gene            1..5624
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /note="myosin IIIA"
                     /db_xref="GeneID:53904"
                     /db_xref="HGNC:HGNC:7601"
                     /db_xref="MIM:606808"
     exon            1..100
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            101..187
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            188..372
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..5055
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myosin-IIIa; epididymis secretory sperm binding protein"
                     /codon_start=1
                     /product="myosin-IIIa isoform 1"
                     /protein_id="NP_059129.3"
                     /db_xref="CCDS:CCDS7148.1"
                     /db_xref="GeneID:53904"
                     /db_xref="HGNC:HGNC:7601"
                     /db_xref="MIM:606808"
                     /translation="MFPLIGKTIIFDNFPDPSDTWEITETIGKGTYGKVFKVLNKKNG
                     QKAAVKILDPIHDIDEEIEAEYNILKALSDHPNVVRFYGIYFKKDKVNGDKLWLVLEL
                     CSGGSVTDLVKGFLKRGERMSEPLIAYILHEALMGLQHLHNNKTIHRDVKGNNILLTT
                     EGGVKLVDFGVSAQLTSTRHRRNTSVGTPFWMAPEVIACEQQLDTTYDARCDTWSLGI
                     TAIELGDGDPPLADLHPMRALFKIPRNPPPKLRQPELWSAEFNDFISKCLTKDYEKRP
                     TVSELLQHKFITQIEGKDVMLQKQLTEFIGIHQCMGGTEKARRERIHTKKGNFNRPLI
                     SNLKDVDDLATLEILDENTVSEQLEKCYSRDQIYVYVGDILIALNPFQSLGLYSTKHS
                     KLYIGSKRTASPPHIFAMADLGYQSMITYNSDQCIVISGESGAGKTENAHLLVQQLTV
                     LGKANNRTLQEKILQVNNLVEAFGNACTIINDNSSRFGKYLEMKFTSSGAVVGAQISE
                     YLLEKSRVIHQAIGEKNFHIFYYIYAGLAEKKKLAHYKLPENKPPRYLQNDHLRTVQD
                     IMNNSFYKSQYELIEQCFKVIGFTMEQLGSIYSILAAILNVGNIEFSSVATEHQIDKS
                     HISNHTALENCASLLCIRADELQEALTSHCVVTRGETIIRPNTVEKATDVRDAMAKTL
                     YGRLFSWIVNCINSLLKHDSSPSGNGDELSIGILDIFGFENFKKNSFEQLCINIANEQ
                     IQYYYNQHVFAWEQNEYLNEDVDARVIEYEDNWPLLDMFLQKPMGLLSLLDEESRFPK
                     ATDQTLVEKFEGNLKSQYFWRPKRMELSFGIHHYAGKVLYNASGFLAKNRDTLPTDIV
                     LLLRSSDNSVIRQLVNHPLTKTGNLPHSKTKNVINYQMRTSEKLINLAKGDTGEATRH
                     ARETTNMKTQTVASYFRYSLMDLLSKMVVGQPHFVRCIKPNSERQARKYDKEKVLLQL
                     RYTGILETARIRRLGFSHRILFANFIKRYYLLCYKSSEEPRMSPDTCATILEKAGLDN
                     WALGKTKVFLKYYHVEQLNLMRKEAIDKLILIQACVRAFLCSRRYQKIQEKRKESAII
                     IQSAARGHLVRKQRKEIVDMKNTAVTTIQTSDQEFDYKKNFENTRESFVKKQAENAIS
                     ANERFISAPNNKGSVSVVKTSTFKPEEETTNAVESNNRVYQTPKKMNNVYEEEVKQEF
                     YLVGPEVSPKQKSVKDLEENSNLRKVEKEEAMIQSYYQRYTEERNCEESKAAYLERKA
                     ISERPSYPVPWLAENETSFKKTLEPTLSQRSIYQNANSMEKEKKTSVVTQRAPICSQE
                     EGRGRLRHETVKERQVEPVTQAQEEEDKAAVFIQSKYRGYKRRQQLRKDKMSSFKHQR
                     IVTTPTEVARNTHNLYSYPTKHEEINNIKKKDNKDSKATSEREACGLAIFSKQISKLS
                     EEYFILQKKLNEMILSQQLKSLYLGVSHHKPINRRVSSQQCLSGVCKGEEPKILRPPR
                     RPRKPKTLNNPEDSTYYYLLHKSIQEEKRRPRKDSQGKLLDLEDFYYKEFLPSRSGPK
                     EHSPSLRERRPQQELQNQCIKANERCWAAESPEKEEEREPAANPYDFRRLLRKTSQRR
                     RLVQQS"
     exon            373..507
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            508..612
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            613..712
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            713..789
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            790..935
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1001
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1157
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1257
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1374
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1479
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1563
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1766
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1865
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1980
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2107
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2318
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2466
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..2620
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2709
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2710..2839
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..2919
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2920..2997
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            2998..3203
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            3204..3315
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            3316..3478
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            3479..3602
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            3603..4497
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            4498..4642
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            4643..4749
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            4750..4790
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            4791..4934
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     exon            4935..5624
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5600..5605
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /note="hexamer: AATAAA"
     polyA_site      5624
                     /gene="MYO3A"
                     /gene_synonym="DFNA90; DFNB30"
                     /note="major polyA site"
ORIGIN      
        1 agttcttccc ctgctcggct gcccctgccc gcccctcgag ggagcgcccg tagccagcgc
       61 ccccgtaaag aaataaggag gcgcccggct tcccgcccag gcccagggcc ccagtgcagc
      121 ctggacacag tattcaaagt tttggcgtct gagcattgtt atgcgttatt ttcaagcttt
      181 gctaacggtt tttaaacctt tgagatgttt ccattaattg gaaaaacaat catctttgat
      241 aactttcctg atccttctga tacatgggaa atcactgaga caattggcaa aggaacttat
      301 gggaaagttt ttaaagtatt gaataagaaa aatggccaaa aagcagcagt caaaattctt
      361 gatccaattc acgatattga cgaagagatt gaagcagaat ataacatctt aaaagcactt
      421 tctgaccacc ctaatgtggt cagattctat gggatatact ttaagaagga taaagtaaat
      481 ggagacaagc tgtggttggt tcttgagctc tgcagtggag gatcagtgac tgaccttgtg
      541 aaaggatttc tgaagagggg tgaaagaatg agtgagcctc taattgccta tattttacat
      601 gaagcactaa tgggacttca acatttgcat aacaacaaaa ctatccacag agatgtgaaa
      661 ggcaataaca ttctattgac cacggaaggt ggagtgaaac tagtagattt tggtgtgtct
      721 gcacagctca ccagtacccg gcaccgtcgg aacacatccg taggaacacc gttttggatg
      781 gctcctgagg tgattgcatg tgaacagcaa ttggatacca cttatgacgc cagatgtgac
      841 acttggtccc tgggtatcac ggccattgag ctgggtgatg gagatcctcc actagctgac
      901 cttcatccca tgagagcact cttcaaaata ccaaggaatc cacccccaaa actaaggcag
      961 cctgagctat ggtcagcaga attcaatgac ttcataagca agtgcttgac taaagattat
     1021 gaaaagcgtc caacagtgtc agaactttta cagcataaat tcattactca aattgagggc
     1081 aaagatgtga tgctacaaaa acaactaacg gaattcattg gcatccatca atgcatggga
     1141 ggcacagaaa aggccagacg tgaacgtatt cacacgaaga aagggaactt caaccgacct
     1201 ctaatatcca atctgaagga tgtagatgat ttagcaaccc tagaaatttt ggatgagaat
     1261 acagtctcag agcaacttga gaagtgttat tccagagatc agatctacgt ctatgtggga
     1321 gacatactca ttgctcttaa cccttttcag agtctgggtc tttactccac aaagcattcc
     1381 aaactatata ttggatcaaa gagaactgcc agtcctcctc acatttttgc aatggctgac
     1441 ttaggatatc aatctatgat aacatataat tcagatcagt gcattgttat ttctggagaa
     1501 agtggtgctg gaaagactga aaatgctcat cttttagttc agcagctgac agtgcttgga
     1561 aaggctaata acagaacctt gcaagagaag attttacaag tgaacaattt ggtagaagcc
     1621 tttggcaatg cctgcactat tataaatgac aattctagca gatttggaaa atacttagaa
     1681 atgaaattca cctcttctgg agcggtagtg ggagcacaga tttctgaata tctcctggaa
     1741 aaatcccgag ttatccacca agctattgga gaaaaaaatt ttcatatttt ttactacatt
     1801 tatgctggtt tggctgaaaa gaagaaacta gcccattaca aactgcctga aaataagcct
     1861 cccaggtacc tacaaaatga ccacctcaga acagtacaag acatcatgaa taatagtttc
     1921 tataaatccc agtatgaatt aattgagcaa tgtttcaaag tcataggttt tacaatggag
     1981 caacttggta gtatatacag catactcgct gcaatcttga atgttggcaa cattgaattt
     2041 tcttctgtgg caactgaaca ccagattgac aagagccaca tttctaatca tacagccctg
     2101 gagaactgtg cttctttgct ttgcattcgg gcagatgagc tacaagaagc tctcacctcc
     2161 cactgtgtgg tcactagagg agaaacaatt atacgaccca atactgtaga aaaagctacc
     2221 gatgtcaggg atgccatggc taaaacttta tatggacgtc tctttagttg gatagtcaat
     2281 tgcattaaca gtttgttgaa gcatgactca tcaccaagtg ggaatggtga tgagctgagc
     2341 attggcattc ttgatatatt tggctttgaa aatttcaaaa aaaattcctt cgagcagctg
     2401 tgcattaaca ttgcaaatga acaaattcag tattattata atcaacatgt gtttgcatgg
     2461 gaacagaatg aatacctaaa tgaagatgtg gatgctagag ttattgaata tgaggataac
     2521 tggcccctct tagatatgtt tctgcaaaag ccaatgggtt tactttccct acttgatgaa
     2581 gaaagtagat ttcccaaggc cactgaccag actcttgtag aaaaatttga aggtaacctg
     2641 aaatcacaat acttctggag acccaaaaga atggaactta gttttggaat tcaccattat
     2701 gcaggaaagg tcctctataa tgcaagtgga ttcttagcca aaaacagaga cactcttcct
     2761 actgacattg tgctactttt gaggtcatcc gacaacagtg taattaggca actagtcaac
     2821 caccctctga ccaaaacagg taatctgcca cattctaaaa ctaaaaatgt tataaactat
     2881 caaatgagga cttcagaaaa attaatcaac ctggcaaagg gcgacactgg agaagccaca
     2941 cgtcatgcca gagagacaac caacatgaaa acacaaacgg ttgcatcata ttttagatat
     3001 tccctgatgg atttgttgtc taaaatggtg gtgggccaac ctcattttgt ccgttgcatc
     3061 aaaccaaata gtgagcgtca ggcaagaaaa tatgacaaag agaaagttct gctacagctt
     3121 cggtacacag gaattctgga aacagcaaga attcgaagac taggattctc ccatcggata
     3181 ctttttgcta actttataaa gcggtactac cttctctgct acaagtcgag cgaggagccc
     3241 cgcatgagcc ctgacacctg tgccaccatt ttggaaaaag ctggtctcga taactgggct
     3301 cttggaaaaa caaaagtgtt ccttaagtat tatcacgtgg agcagttaaa tctaatgcga
     3361 aaggaagcta ttgacaagct tattttgatt caagcttgtg tcagagcatt cttgtgttca
     3421 agaagatacc aaaaaataca ggagaaaagg aaagaaagcg ctataataat acagtcagct
     3481 gcaagaggac accttgtcag gaaacaaaga aaagaaattg ttgacatgaa aaacacagca
     3541 gtaacaacca ttcaaacttc tgatcaggaa ttcgactaca agaaaaactt tgaaaataca
     3601 agggaatctt tcgtgaagaa acaagcagaa aatgcaatct ctgctaatga aagattcatt
     3661 tcagctccaa ataataaagg aagtgtatct gtagtgaaga cttccacttt caaacctgaa
     3721 gaggaaacca ccaatgctgt ggagagtaac aacagagtgt atcagactcc aaaaaaaatg
     3781 aataatgtgt atgaggaaga ggttaagcaa gaattctacc ttgtagggcc agaagtaagc
     3841 cccaaacaga agtctgtcaa agacctggaa gagaacagca atctaaggaa agtggagaaa
     3901 gaggaagcta tgatccagag ttactatcag aggtacacag aggagaggaa ttgtgaagag
     3961 tcaaaagcag catatctaga aaggaaggcc atatcagaaa ggccaagcta cccagtgcct
     4021 tggttagctg aaaatgagac ttcctttaaa aaaactttgg aacctacact tagccaaagg
     4081 tcaatttatc aaaatgcaaa cagcatggaa aaagaaaaga agacatctgt agttacccag
     4141 cgtgcaccga tatgcagcca ggaggaaggc agaggccgtc tgaggcatga gacagtcaaa
     4201 gagaggcaag ttgaaccagt gacacaggcc caggaggaag aagataaagc agcggtattc
     4261 attcagagca aataccgggg ttacaagaga aggcagcagt tgaggaagga caagatgtct
     4321 tcttttaagc atcagaggat tgtcacaaca ccaacagaag tagcaagaaa cactcataat
     4381 ttgtattcct atcccacaaa acatgaggaa atcaataaca tcaagaagaa ggataacaaa
     4441 gactcgaaag caacttcaga aagagaagca tgtggtttgg caattttttc aaaacagata
     4501 tcaaagttat ctgaagaata tttcattctg cagaaaaaat tgaatgaaat gattttgtca
     4561 cagcaactga agtcacttta tctgggtgtc tcgcaccata agccaattaa tagacgagtt
     4621 tcttctcagc agtgcctctc aggtgtctgt aaaggagagg agccaaaaat attgagaccc
     4681 ccaagacgac cccggaaacc caaaacatta aataaccctg aagactccac atactattat
     4741 ctacttcata agtcaatcca agaagaaaaa cgaagaccaa ggaaagacag tcagggaaaa
     4801 ttattagatt tggaagattt ctattataag gaatttttgc ccagtcgttc tggaccaaag
     4861 gaacatagcc ctagtttaag agaacgaaga ccacagcaag aactccagaa tcaatgtatt
     4921 aaggctaatg aaaggtgctg ggcggcggag agccccgaga aggaggagga gagagagcca
     4981 gcagccaacc cctacgactt caggaggctc ctgcgcaaaa cctcccagcg ccggcgcctc
     5041 gtccagcagt cctaaccgtt caacgaggca gtcaccgccg tcggaaggcg ctggagcctg
     5101 cggggcagca ggggccaagc aggcactctg gggctggcac cagcaggcac tgaagctgcg
     5161 gccctgatct ccgcagaggc tgcctgctgc gctcggccct caagtgcccg ggccggcctt
     5221 cgtgctccga aacaagagac ctgggagccc tcgggaaacc tcccccgacg ctctctctcg
     5281 gaactcccgc accctccttt ctcaccagcc cgccagttgt ggcaaccctg tccttgttcc
     5341 cctaatctat cactttgttc tttttttttg tgactcctgt ggactccact gcgcctggga
     5401 tctcgccaac ccctctctca tttggggtga ctgaattcac agattttttt ttttattgga
     5461 aacggctttt cttggccaac agaacacttg ctagcggttg aatcttagag aaaaaagccc
     5521 gggaggggtg gggagaattt cgaagatgta tttcatctca agcttgctct ttctcttcct
     5581 ttggttatta aggtcactaa ataaaggaag tgccttggaa aacc
//
