LOCUS       NM_004998               8644 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens myosin IE (MYO1E), mRNA.
ACCESSION   NM_004998
VERSION     NM_004998.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Jusue-Torres,I., Tiv,R., Ricarte-Filho,J.C., Mallisetty,A.,
            Contreras-Vargas,L., Godoy-Calderon,M.J., Khaddour,K., Kennedy,K.,
            Valyi-Nagy,K., David,O., Menchaca,M., Kottorou,A., Koutras,A.,
            Dimitrakopoulos,F., Abdelhady,K.M., Massad,M., Rubinstein,I.,
            Feldman,L., Stewart,J., Shimamura,T., Danilova,L. and Hulbert,A.
  TITLE     Myo1e overexpression in lung adenocarcinoma is associated with
            increased risk of mortality
  JOURNAL   Sci Rep 13 (1), 4107 (2023)
   PUBMED   36914720
  REMARK    GeneRIF: Myo1e overexpression in lung adenocarcinoma is associated
            with increased risk of mortality.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Krendel,M., Leh,S., Garone,M.E., Edwards-Richards,A., Lin,J.J.,
            Brackman,D., Knappskog,P. and Mikhailov,A.
  TITLE     Focal segmental glomerulosclerosis and proteinuria associated with
            Myo1E mutations: novel variants and histological phenotype analysis
  JOURNAL   Pediatr Nephrol 38 (2), 439-449 (2023)
   PUBMED   35723736
  REMARK    GeneRIF: Focal segmental glomerulosclerosis and proteinuria
            associated with Myo1E mutations: novel variants and histological
            phenotype analysis.
REFERENCE   3  (bases 1 to 8644)
  AUTHORS   Liu,P.J., Gunther,L.K., Garone,M.E., Zhang,C., Perez,D.,
            Bi-Karchin,J., Pellenz,C.D., Chase,S.E., Presti,M.F., Plante,E.L.,
            Martin,C.E., Lovric,S., Yengo,C.M., Hildebrandt,F. and Krendel,M.
  TITLE     Steroid-Resistant Nephrotic Syndrome-Associated MYO1E Mutations
            Have Differential Effects on Myosin 1e Localization, Dynamics, and
            Activity
  JOURNAL   J Am Soc Nephrol 33 (11), 1989-2007 (2022)
   PUBMED   36316095
  REMARK    GeneRIF: Steroid-Resistant Nephrotic Syndrome-Associated MYO1E
            Mutations Have Differential Effects on Myosin 1e Localization,
            Dynamics, and Activity.
REFERENCE   4  (bases 1 to 8644)
  AUTHORS   Zhang,Y., Cao,F., Zhou,Y., Feng,Z., Sit,B., Krendel,M. and Yu,C.H.
  TITLE     Tail domains of myosin-1e regulate phosphatidylinositol signaling
            and F-actin polymerization at the ventral layer of podosomes
  JOURNAL   Mol Biol Cell 30 (5), 622-635 (2019)
   PUBMED   30601698
  REMARK    GeneRIF: TH12 domain of Myo1e serves as a regulatory component to
            connect phosphatidylinositol signaling to F-actin polymerization at
            the podosome.
REFERENCE   5  (bases 1 to 8644)
  AUTHORS   El Mezgueldi,M., Tang,N., Rosenfeld,S.S. and Ostap,E.M.
  TITLE     The kinetic mechanism of Myo1e (human myosin-IC)
  JOURNAL   J Biol Chem 277 (24), 21514-21521 (2002)
   PUBMED   11940582
  REMARK    GeneRIF: The kinetic mechanism of Myo1e (human myosin-IC).
REFERENCE   6  (bases 1 to 8644)
  AUTHORS   Skowron,J.F., Bement,W.M. and Mooseker,M.S.
  TITLE     Human brush border myosin-I and myosin-Ic expression in human
            intestine and Caco-2BBe cells
  JOURNAL   Cell Motil Cytoskeleton 41 (4), 308-324 (1998)
   PUBMED   9858156
REFERENCE   7  (bases 1 to 8644)
  AUTHORS   Robertson,H., Langdon,W.Y., Thien,C.B. and Bowtell,D.D.
  TITLE     A c-Cbl yeast two hybrid screen reveals interactions with 14-3-3
            isoforms and cytoskeletal components
  JOURNAL   Biochem Biophys Res Commun 240 (1), 46-50 (1997)
   PUBMED   9367879
REFERENCE   8  (bases 1 to 8644)
  AUTHORS   Hasson,T., Skowron,J.F., Gilbert,D.J., Avraham,K.B., Perry,W.L.,
            Bement,W.M., Anderson,B.L., Sherr,E.H., Chen,Z.Y., Greene,L.A.,
            Ward,D.C., Corey,D.P., Mooseker,M.S., Copeland,N.G. and
            Jenkins,N.A.
  TITLE     Mapping of unconventional myosins in mouse and human
  JOURNAL   Genomics 36 (3), 431-439 (1996)
   PUBMED   8884266
REFERENCE   9  (bases 1 to 8644)
  AUTHORS   Bement,W.M., Wirth,J.A. and Mooseker,M.S.
  TITLE     Cloning and mRNA expression of human unconventional myosin-IC. A
            homologue of amoeboid myosins-I with a single IQ motif and an SH3
            domain
  JOURNAL   J Mol Biol 243 (2), 356-363 (1994)
   PUBMED   7932763
REFERENCE   10 (bases 1 to 8644)
  AUTHORS   Bement,W.M., Hasson,T., Wirth,J.A., Cheney,R.E. and Mooseker,M.S.
  TITLE     Identification and overlapping expression of multiple
            unconventional myosin genes in vertebrate cell types
  JOURNAL   Proc Natl Acad Sci U S A 91 (14), 6549-6553 (1994)
   PUBMED   8022818
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11767. doi:
            10.1073/pnas.91.24.11767-c.. PMID: 7972138]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092868.2, BC098392.1 and
            AC092757.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_004998.3.
            
            Summary: This gene encodes a member of the nonmuscle class I
            myosins which are a subgroup of the unconventional myosin protein
            family. The unconventional myosin proteins function as actin-based
            molecular motors. Class I myosins are characterized by a head
            (motor) domain, a regulatory domain and a either a short or long
            tail domain. Among the class I myosins, this protein is
            distinguished by a long tail domain that is involved in
            crosslinking actin filaments. This protein localizes to the
            cytoplasm and may be involved in intracellular movement and
            membrane trafficking. Mutations in this gene are the cause of focal
            segmental glomerulosclerosis-6. This gene has been referred to as
            myosin IC in the literature but is distinct from the myosin IC gene
            located on chromosome 17. [provided by RefSeq, Jan 2012].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098392.1, SRR1803611.85379.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000288235.9/ ENSP00000288235.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-268               AC092868.2         107251-107518       c
            269-4116            BC098392.1         269-4116
            4117-4117           AC092757.2         151396-151396       c
            4118-5109           BC098392.1         4118-5109
            5110-8644           AC092757.2         146869-150403       c
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.2"
     gene            1..8644
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /note="myosin IE"
                     /db_xref="GeneID:4643"
                     /db_xref="HGNC:HGNC:7599"
                     /db_xref="MIM:601479"
     exon            1..374
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     CDS             372..3698
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /note="myosin-IC; MYO1E variant protein; unconventional
                     myosin 1E"
                     /codon_start=1
                     /product="unconventional myosin-Ie"
                     /protein_id="NP_004989.2"
                     /db_xref="CCDS:CCDS32254.1"
                     /db_xref="GeneID:4643"
                     /db_xref="HGNC:HGNC:7599"
                     /db_xref="MIM:601479"
                     /translation="MGSKGVYQYHWQSHNVKHSGVDDMVLLSKITENSIVENLKKRYM
                     DDYIFTYIGSVLISVNPFKQMPYFGEKEIEMYQGAAQYENPPHIYALADNMYRNMIID
                     RENQCVIISGESGAGKTVAAKYIMSYISRVSGGGTKVQHVKDIILQSNPLLEAFGNAK
                     TVRNNNSSRFGKYFEIQFSPGGEPDGGKISNFLLEKSRVVMRNPGERSFHIFYQLIEG
                     ASAEQKHSLGITSMDYYYYLSLSGSYKVDDIDDRREFQETLHAMNVIGIFAEEQTLVL
                     QIVAGILHLGNISFKEVGNYAAVESEEFLAFPAYLLGINQDRLKEKLTSRQMDSKWGG
                     KSESIHVTLNVEQACYTRDALAKALHARVFDFLVDSINKAMEKDHEEYNIGVLDIYGF
                     EIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIEYFNNKIVCDLI
                     ENKVNPPGIMSILDDVCATMHAVGEGADQTLLQKLQMQIGSHEHFNSWNQGFIIHHYA
                     GKVSYDMDGFCERNRDVLFMDLIELMQSSELPFIKSLFPENLQADKKGRPTTAGSKIK
                     KQANDLVSTLMKCTPHYIRCIKPNETKKPRDWEESRVKHQVEYLGLKENIRVRRAGYA
                     YRRIFQKFLQRYAILTKATWPSWQGEEKQGVLHLLQSVNMDSDQFQLGRSKVFIKAPE
                     SLFLLEEMRERKYDGYARVIQKSWRKFVARKKYVQMREEASDLLLNKKERRRNSINRN
                     FIGDYIGMEEHPELQQFVGKREKIDFADTVTKYDRRFKGVKRDLLLTPKCLYLIGREK
                     VKQGPDKGLVKEVLKRKIEIERILSVSLSTMQDDIFILHEQEYDSLLESVFKTEFLSL
                     LAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGSRQVQFHQGFGDLAVLKPSNK
                     VLQVSIGPGLPKNSRPTRRNTTQNTGYSSGTQNANYPVRAAPPPPGYHQNGVIRNQYV
                     PYPHAPGSQRSNQKSLYTSMARPPLPRQQSTSSDRVSQTPESLDFLKVPDQGAAGVRR
                     QTTSRPPPAGGRPKPQPKPKPQVPQCKALYAYDAQDTDELSFNANDIIDIIKEDPSGW
                     WTGRLRGKQGLFPNNYVTKI"
     exon            375..518
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            519..608
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            609..703
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            704..791
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            792..881
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1013
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1148
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1281
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1282..1478
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1559
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1646
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1733
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..1901
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..1987
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..2069
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2176
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2177..2275
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2420
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2535
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2705
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2706..2851
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2998
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            2999..3156
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            3157..3249
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            3250..3451
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            3452..3621
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     exon            3622..8644
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4695..4700
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /note="hexamer: AATAAA"
     polyA_site      4714
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /note="major polyA site"
     regulatory      5087..5092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /note="hexamer: AATACA"
     polyA_site      5109
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
     regulatory      6144..6149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /note="hexamer: ATTAAA"
     polyA_site      6164
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
     regulatory      8620..8625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
                     /note="hexamer: AGTAAA"
     polyA_site      8644
                     /gene="MYO1E"
                     /gene_synonym="FSGS6; HuncM-IC; MYO1C"
ORIGIN      
        1 gagactcagc cagaggagta cggattaagt ggccgaagaa agaggagtga ggcaggggag
       61 cgaggcggac gctccgagcg catcggactc acctgggccg gcggcgaccg gcgagtacga
      121 gtggactctt gccctccgca gcatgcgccc cgcctgctaa gtcgccgccg tctcgccctc
      181 acctagcacc gccaatcccg cctggatgga gtcccctctg ggttcccatt ggctcctgtg
      241 agtgcttggg tgtcgggagc ctgtttttgg cgaggggaac caacttttga agttcgccca
      301 gaagactgcg ttccagcccc agttccccgg cagcgggacc cggcgaagac gcgaccgcgg
      361 cgcgagtcac catgggaagc aaaggtgtct accagtacca ctggcaaagc cacaatgtca
      421 agcacagtgg tgtggacgac atggtgctac tgtccaagat cacagagaac tccatcgtgg
      481 agaatctgaa gaagagatac atggatgact acatttttac atatatagga tctgtattaa
      541 tctcagtcaa ccctttcaag cagatgccat attttgggga aaaggaaatt gaaatgtacc
      601 aaggagcggc acagtatgaa aacccaccac atatctatgc ccttgcagat aatatgtaca
      661 gaaacatgat cattgacaga gagaaccagt gcgtcattat cagtggtgaa agtggtgctg
      721 gaaaaacagt ggctgccaaa tatatcatga gctacatctc cagagtgtct ggaggaggga
      781 ccaaagtcca gcacgtgaag gacattatcc tgcagtccaa cccgctgctg gaggccttcg
      841 ggaacgccaa gaccgtccgg aacaacaact ccagccgatt tggaaaatac tttgaaatcc
      901 agttcagtcc aggtggggaa ccagatggtg gaaagatctc caacttcctt ctggaaaaat
      961 ctagggtggt gatgaggaac ccaggagagc ggagttttca catattttac cagctcatcg
     1021 agggcgcctc tgcagagcag aaacacagcc ttggcatcac cagcatggac tattattact
     1081 acctgagcct ctcgggctca tacaaggttg atgacattga cgacaggcgg gagtttcagg
     1141 aaactctgca cgccatgaat gtgattggga tctttgcaga agagcaaacg ctggtgttgc
     1201 agatagtggc gggtattctc cacctgggaa acatcagctt caaagaagtt ggcaactacg
     1261 cggctgtgga gagtgaagag tttttagctt ttcctgcata tctgctaggg ataaaccagg
     1321 accggttgaa agaaaagcta acaagccggc agatggatag caagtgggga ggcaaatccg
     1381 aatccatcca cgtgaccctc aacgtagagc aggcctgtta cacccgggat gcgctcgcca
     1441 aggccctgca cgcccgggtc tttgatttct tggtagattc catcaataaa gccatggaga
     1501 aagaccatga agaatacaac attggcgtcc tagacatcta tggctttgaa atattccaga
     1561 aaaatggctt tgaacagttt tgtatcaatt ttgttaatga aaaactgcag cagattttta
     1621 ttgaactgac attaaaggca gaacaggaag aatatgttca agagggaata agatggacac
     1681 ccattgagta ctttaataat aaaatcgtat gtgacctcat agagaacaaa gtgaaccctc
     1741 ctggcatcat gagcatcctg gatgacgtgt gcgccacgat gcatgcggtg ggtgaggggg
     1801 cagatcagac gctgctccag aaacttcaga tgcagattgg gagtcatgag cacttcaaca
     1861 gttggaacca aggcttcatc attcatcatt atgctgggaa ggtatcctat gacatggatg
     1921 gcttttgtga aaggaaccgg gatgtgcttt ttatggatct catcgagctt atgcagagca
     1981 gcgagctgcc tttcataaag tctttatttc cggaaaatct gcaggctgac aagaaagggc
     2041 gcccaactac tgccggaagc aaaataaaga aacaagccaa tgaccttgtg agcaccctga
     2101 tgaaatgtac gccccactac attcgctgca tcaagccaaa cgaaaccaag aagcccagag
     2161 actgggagga aagcagggta aagcatcaag tcgaatattt gggtctgaaa gagaacattc
     2221 gagtgagaag agctggctat gcctatcggc gcatcttcca aaaattccta cagaggtatg
     2281 ccattctgac caaagccacc tggccttctt ggcagggaga ggagaagcaa ggcgtcctgc
     2341 acctgctgca gtcggtcaac atggacagcg accagttcca gctggggagg agtaaagtgt
     2401 tcatcaaagc ccccgagtct ctatttcttt tagaagagat gagagagaga aagtatgatg
     2461 ggtatgctcg agtgatacag aaatcatgga ggaaattcgt ggcccggaag aaatacgttc
     2521 aaatgagaga agaagcctca gacctcttat tgaacaagaa ggagagaagg agaaacagta
     2581 ttaacaggaa ctttataggg gattatattg ggatggaaga gcacccagaa ctccagcagt
     2641 tcgtgggcaa gagggagaag attgatttcg cagacacagt caccaagtat gacaggaggt
     2701 tcaagggtgt aaagcgagac ctgctcctta ccccaaagtg cttgtactta atcggacgag
     2761 aaaaagtcaa acagggccca gacaagggcc tggtgaaaga agtcctgaag cggaaaatcg
     2821 agatagaacg gatcttgtct gtgtccctca gtactatgca ggatgacatt tttattctcc
     2881 atgagcaaga gtatgacagt ttgcttgaat ctgtcttcaa aactgaattc ctaagcctct
     2941 tagcaaagcg ttacgaggag aagacccaga agcaactacc tctgaaattc agcaatacgc
     3001 ttgaactgaa gttgaaaaag gaaaactggg gcccctggag tgcagggggc tcccggcaag
     3061 tgcagttcca ccaagggttt ggggacctgg ctgtcctcaa gcccagtaac aaagtgctgc
     3121 aggtcagcat cggacctgga ctgcccaaga actcccgtcc taccagaagg aacactaccc
     3181 aaaatacagg ttattccagt gggactcaaa atgccaacta cccagtgaga gctgcccctc
     3241 ctcccccagg ataccatcag aacggagtca tcagaaacca gtatgtgcca tatccccatg
     3301 ctcctggaag ccagaggtcc aatcagaaaa gcctgtacac ctccatggcc cgcccgccct
     3361 tgcctcggca gcagtctacc agttcagacc gagtgtcaca gacgccagag agcctggatt
     3421 tcctcaaggt cccggaccag ggagctgcag gggtcaggag acaaacaacc agtcggcctc
     3481 ccccagcagg gggcagaccc aagccccagc ccaagcccaa gcctcaggtg ccacagtgca
     3541 aggctttgta tgcctatgac gctcaggaca cagacgaact cagctttaat gccaatgaca
     3601 ttattgatat tatcaaagaa gatccttctg gctggtggac gggtcgacta cgaggcaagc
     3661 agggcctgtt ccccaacaac tatgtgacca agatctgagg tgcccgtgac tctgacacat
     3721 ggggcagagg agctccaggc acagaccagg ggaggggata tttaggggct ccccttacaa
     3781 tccacaatga gcaattgctt ctccaaggcc tggagctatt ctggtacctt ccccatggag
     3841 gacactgaaa aggctgggtt ggggacaggg agtatcactc cataagtgat cctaaaaggt
     3901 agcctcttca taggaaccca ggaggacaaa accaccatgc attaagattt atttattgta
     3961 tttaaacctg gtgagaggac aagtgaggtc tgctcagacc ttgtaggctt ctatcaaaac
     4021 agcaccctgc ttgctcacca ggcctagaga atggctgtag gtggccgctg acaagtgcct
     4081 ttagttgaag agcacatttc tttcatctct cttgtccata cctgatagac acattcctct
     4141 ctgccacctt ccttcaggga ggacccgccc tctgcagact gggcttagcg tgagcaggca
     4201 cttcccatgt acgtgccaag ggtaagctgg cctgctgagc ccagggcgac agaggggcac
     4261 tggtttacac tttgccggga ccatcagggc cgccaagcag gtcaggggct gggggctggg
     4321 ggctgggctg ctggctttgc tttctctggg tcttcaatta gaatgtggct ggcccatatt
     4381 ggtttgtgtt taaatgctgt acttactaca agaaggatct tttttcaagc tgtacattta
     4441 taaaaacaga tcatatactg tatatataaa aatcttgaga tggtagaaac atgtatgaat
     4501 gtactaagta gtattccact gtactcattc ataaggtagg ttttcttaca aaactcacac
     4561 caggtactta aagatgtgct ctgctttttt ccaactacgg agtgtcactg ctttctaggt
     4621 cagtccctgc agactcttct caactctttc cctataggaa acttactccg cgtcctgccc
     4681 ccacctccta aataaataaa ggaatcggcg aacaccttct tcttttatga catttgttat
     4741 gggttgaatt gtgtgcctcc aaagaagatg ttggagtccc agccaccagt acctcacaat
     4801 atgaccttat ttggaaatag agtctttatg gagcaaacaa acaaaactaa ttaaaatgaa
     4861 gtcattagag tgggtcttaa tccaatataa tggtgtcctg atgaaaaggg gaaatttgga
     4921 cacagagacc aacatgccag aggaaagacc atgtgaaaag gcagggagaa gacagccatt
     4981 cacaagccct ggacagaggc ctggaacaga ttctccctca caccctcaca aggaaccagc
     5041 cctgcccacc ttggtcttgg acttccaacc tccagaactg tgaggcaata catttcgctg
     5101 tttaagccac tcggtttgtg gtagtttgtt acggcagccc taacaaacta aaacaatgtt
     5161 ttcttgccat aatagtgaaa agctggaatc agtgtaatgc ccatcaataa tggaatggtt
     5221 gtaagtagag actgccattt ctctaccttt cctcctctgg gtgccatagc cattcttaag
     5281 ctactggtaa cttagagttt gagcagcata cacctttgag gctttcagga tcctcaagtc
     5341 catgggaaaa aattatcagg gcttacgtat aagggaaaaa aattgatcta gatttgaaac
     5401 gaagatacat ttaattctgc tgtgttttac ttcaagaaca gggagcaaac tccattctgt
     5461 aacagtaacg aataatgtta aaaggctact tcagttccat gttgcgacca gagaggaaag
     5521 tagagccatg atgcggtgag ttgaggaaag gtaatcctgt tgacactagt ggtctctaaa
     5581 gtactttcac attcatggac tccccgcagt gggaggaaga taccaccaga actgggacag
     5641 aggagcatgc tggagtcccc acagccacca gcactcaggc tgcgttctca gggcaggaac
     5701 accaggacca tcttcccttg ttatcataag cccctggctg gctgggacaa gcctttgttt
     5761 ttcctcctga ttgattgatt ggcattttac acttgtgtgt aactgggaac catcgggctc
     5821 taggtcacaa aacagggact gagttaaacc cccacgagta acaccctcat ccatggaaat
     5881 cacagaccaa gcccttagca cctgcatcgt gtgcccagcc agtgccaggc tcaaggagat
     5941 gcccaacaaa tggtgctgaa ataggtaagt gacccacagg ggaaggagac ttagatctgc
     6001 gtagagatgc tgaaatgctg acactattaa gaaaagtttg gagaaatagt ggtttatctt
     6061 aaacttattt gtatatattt agaaattcaa catggggact accagaaaaa tctgggataa
     6121 ataacatttc cctttttttc ctaattaaaa agtcttcaga aaaatgtctt ttttcccttt
     6181 aagacactaa cctgggtttc ctctagtctg tggatctagc ctggcctgcc attttgttca
     6241 ctgtgcagtt aagcaaatat gaccacttta ggagtcactt accaggaaag aacatacaga
     6301 agatcaaggg gccaccccca ccttctggaa cttgccatcc tgtttgaaat aaggggtgtt
     6361 catgtgcaca cacagcaacg tgtatagcag tatcaaacac agtataaaaa tgtgacatcc
     6421 cttcaaccaa aggttttaag taaccaggaa ctaaatgaga aggcctgact gtgtacccta
     6481 gggtagaggt gttcaagctt tagttgtaga agagagagag acagaggtct gtataagggt
     6541 ggtatccact ctactgatgg ccgtctgtga gacagagaga ggctgatctg aaactcctgg
     6601 gatcaaacga tcctcctgcc tcagcctctc aaagtgctgg gattatgggc aagagccacc
     6661 gtacctggcc aactggctta aagacatgca aaagggccgt ctcttccaca gtgttttgtg
     6721 aaacaataca gagacatgtg gagaggacaa agggtgaagc cttcttgaat aagcttgctc
     6781 cccaaccatg aaggatgcca acccactgac ctcccaacgt gtggtcccag cccatcgtaa
     6841 tcaaacctga ctccccacta ctaccaagtc cagcccaatg aaggtagaaa agcatcgtgc
     6901 agtttcctcc ttaaaggacc tgccctgtgt gcacaccatg tccaccaata ttctgctggc
     6961 cagagcttag tcatgtggcc tcaactcaag ggggctggag gtctctgcac agagctgtct
     7021 cagcagctta cccagagcac cagagtgaca gcgggctcct gccaggtgga agtgttcaca
     7081 aaaatgagta agagaaaata tggatgtgcc tttatgaatt acaaactcca tgggcttcca
     7141 tagagcatgg cttttacata atctaaattc cttctctttg cgactccctc ctgaactcat
     7201 ccccagaccc tccctgagtg gagctggtca caccaagtca gcctgtgcct ctagttgcta
     7261 ccatttcttt tataggtgtt gtcccagtta agatttcact ggcagtaggg ctggattctg
     7321 gtttccaggg caatcaagag cagagcatga acggtgcgtc tttggtagac aatttaactc
     7381 tgtgtttctg gcacccctca cttttgtgat caaaagaaag accagaggag cctttctgca
     7441 attctgccct ctttccctgt tttgcttttc tcccaaggcc tagctgattg attgcagttc
     7501 tttctggatt tagtaacttg gttttattcc tacccactaa tcttgatgtc tttttcccta
     7561 ctagatcata cctttccact ctgagcaccc tttctaacaa gaagactggc tcagtatggt
     7621 tcactaggtc gcccaaggtc acacagttct tccctggggg agccatcaca ggaactctga
     7681 gtcaaaggcc tgtgcgtttt tattttcaga cagcacctca ctttgtcacc cagactagag
     7741 aacagtggtg cgatcatgcc ttactgcagt ctcaaactcc agggctcagg tgatcctccc
     7801 acctcagcct cccaagtagc tgggaccaca agcatgtgcc accacattcg gctaattatt
     7861 tgtacagatg gggtctccct gtgttgccca gctggtctca aagctctggg ctcaagcagt
     7921 cttcccgcct tggcctccca aaattctggg attacaagca tgagccactg tgcccaaaag
     7981 gcctgtgctt ttaatcattt tctccaattc aacagatatt tagtaatgtg atggtctact
     8041 gtgtcagcct ttcaacttcc aatatcttcc tctcagaaca taaaagtcta tttaaaaaga
     8101 gagagagaag aaaactcacg cataaaactc aacagctaga aacttgtaag ctcctttaat
     8161 gggcaggagc tccatctaac aaatctgaat cctctgattc atctctttcc aagaccatta
     8221 accttctctt taaaataatt aacagctatg ttcaagtatc ttcttcacac tagaagctat
     8281 actaagtgct tggcatactt tacttagcac caccctatga gggagacact cttatcccca
     8341 cttcacaggt acagaaacta aagtatagag atcaccacta cttaaaatca aacccaaagc
     8401 acccacgttc agagttagtg ctcttctata ctatggatac atcatctcat ttttaatttc
     8461 cgtgttttta tgatatggtt tctacttctg ccactcattt cccatttatg acattttagt
     8521 ttggggtgcc tgctgtgcca tctaagaaag tatcttaaag gtcttttttt atgacatcct
     8581 ggaagaattt cctaagtcag gacatttaat atttaactaa gtaaaatatg taataaggaa
     8641 gcta
//
