LOCUS       NM_032608               8534 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens myosin XVIIIB (MYO18B), transcript variant 2, mRNA.
ACCESSION   NM_032608
VERSION     NM_032608.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Cao,C., Zhang,C., Sun,Y., Mu,Z. and Shen,Q.
  TITLE     Myosin18B predicts favorable prognosis of cutaneous squamous-cell
            carcinoma
  JOURNAL   Genes Genomics 43 (4), 371-378 (2021)
   PUBMED   33555505
  REMARK    GeneRIF: Myosin18B predicts favorable prognosis of cutaneous
            squamous-cell carcinoma.
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Altuame,F.D., Haldeman-Englert,C., Cupler,E., Al Muhaizea,M.A.,
            Al-Zaidan,H.I., Hashem,M. and Alkuraya,F.S.
  TITLE     Further delineation of MYO18B-related autosomal recessive
            Klippel-Feil syndrome with myopathy and facial dysmorphism
  JOURNAL   Am J Med Genet A 185 (2), 370-376 (2021)
   PUBMED   33179433
  REMARK    GeneRIF: Further delineation of MYO18B-related autosomal recessive
            Klippel-Feil syndrome with myopathy and facial dysmorphism.
REFERENCE   3  (bases 1 to 8534)
  AUTHORS   Latham,S.L., Weiss,N., Schwanke,K., Thiel,C., Croucher,D.R.,
            Zweigerdt,R., Manstein,D.J. and Taft,M.H.
  TITLE     Myosin-18B Regulates Higher-Order Organization of the Cardiac
            Sarcomere through Thin Filament Cross-Linking and Thick Filament
            Dynamics
  JOURNAL   Cell Rep 32 (9), 108090 (2020)
   PUBMED   32877672
  REMARK    GeneRIF: Myosin-18B Regulates Higher-Order Organization of the
            Cardiac Sarcomere through Thin Filament Cross-Linking and Thick
            Filament Dynamics.
REFERENCE   4  (bases 1 to 8534)
  AUTHORS   Jiu,Y., Kumari,R., Fenix,A.M., Schaible,N., Liu,X., Varjosalo,M.,
            Krishnan,R., Burnette,D.T. and Lappalainen,P.
  TITLE     Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation
            of Contractile Actomyosin Bundles
  JOURNAL   Curr Biol 29 (1), 81-92 (2019)
   PUBMED   30581023
  REMARK    GeneRIF: The results reveal a critical role for myosin-18B in
            myosin II stack assembly and provide evidence that myosin II stacks
            are important for a variety of vital processes in cells.
REFERENCE   5  (bases 1 to 8534)
  AUTHORS   Zhang,Z., Zhu,J., Huang,Y., Li,W. and Cheng,H.
  TITLE     MYO18B promotes hepatocellular carcinoma progression by activating
            PI3K/AKT/mTOR signaling pathway
  JOURNAL   Diagn Pathol 13 (1), 85 (2018)
   PUBMED   30390677
  REMARK    GeneRIF: MYO18B promoted hepatocellular carcinoma growth and
            migration via the activation of PI3K/AKT/mTOR signaling pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8534)
  AUTHORS   Yanaihara,N., Nishioka,M., Kohno,T., Otsuka,A., Okamoto,A.,
            Ochiai,K., Tanaka,T. and Yokota,J.
  TITLE     Reduced expression of MYO18B, a candidate tumor-suppressor gene on
            chromosome arm 22q, in ovarian cancer
  JOURNAL   Int J Cancer 112 (1), 150-154 (2004)
   PUBMED   15305387
  REMARK    GeneRIF: MYO18B alterations, including both epigenetic and genetic
            alterations, play an important role in ovarian carcinogenesis
REFERENCE   7  (bases 1 to 8534)
  AUTHORS   Salamon,M., Millino,C., Raffaello,A., Mongillo,M., Sandri,C.,
            Bean,C., Negrisolo,E., Pallavicini,A., Valle,G., Zaccolo,M.,
            Schiaffino,S. and Lanfranchi,G.
  TITLE     Human MYO18B, a novel unconventional myosin heavy chain expressed
            in striated muscles moves into the myonuclei upon differentiation
  JOURNAL   J Mol Biol 326 (1), 137-149 (2003)
   PUBMED   12547197
  REMARK    GeneRIF: Human MYO18B, a novel unconventional myosin heavy chain
            expressed in striated muscles moves into the myonuclei upon
            differentiation
REFERENCE   8  (bases 1 to 8534)
  AUTHORS   Yokota,J., Nishioka,M., Tani,M. and Kohno,T.
  TITLE     Genetic alterations responsible for metastatic phenotypes of lung
            cancer cells
  JOURNAL   Clin Exp Metastasis 20 (3), 189-193 (2003)
   PUBMED   12741677
  REMARK    Review article
REFERENCE   9  (bases 1 to 8534)
  AUTHORS   Nishioka,M., Kohno,T., Tani,M., Yanaihara,N., Tomizawa,Y.,
            Otsuka,A., Sasaki,S., Kobayashi,K., Niki,T., Maeshima,A.,
            Sekido,Y., Minna,J.D., Sone,S. and Yokota,J.
  TITLE     MYO18B, a candidate tumor suppressor gene at chromosome 22q12.1,
            deleted, mutated, and methylated in human lung cancer
  JOURNAL   Proc Natl Acad Sci U S A 99 (19), 12269-12274 (2002)
   PUBMED   12209013
  REMARK    GeneRIF: candidate tumor suppressor gene at chromosome 22q12.1,
            deleted, mutated, and methylated in human lung cancer
REFERENCE   10 (bases 1 to 8534)
  AUTHORS   Dunham,I., Shimizu,N., Roe,B.A., Chissoe,S., Hunt,A.R.,
            Collins,J.E., Bruskiewich,R., Beare,D.M., Clamp,M., Smink,L.J.,
            Ainscough,R., Almeida,J.P., Babbage,A., Bagguley,C., Bailey,J.,
            Barlow,K., Bates,K.N., Beasley,O., Bird,C.P., Blakey,S.,
            Bridgeman,A.M., Buck,D., Burgess,J., Burrill,W.D., O'Brien,K.P. et
            al.
  TITLE     The DNA sequence of human chromosome 22
  JOURNAL   Nature 402 (6761), 489-495 (1999)
   PUBMED   10591208
  REMARK    Erratum:[Nature 2000 Apr 20;404(6780):904]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB075376.1, AJ310931.2 and
            AL079300.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_032608.6.
            
            Summary: The protein encoded by this gene may regulate
            muscle-specific genes when in the nucleus and may influence
            intracellular trafficking when in the cytoplasm. The encoded
            protein functions as a homodimer and may interact with F actin.
            Mutations in this gene are associated with lung cancer. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 5' coding region compared to variant 1. The
            encoded isoform (2) is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ310931.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149004, SAMEA2151741
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000335473.12/ ENSP00000334563.8
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2195              AB075376.1         45-2239
            2196-2988           AJ310931.2         2231-3023
            2989-6685           AB075376.1         3033-6729
            6686-8530           AJ310931.2         6721-8565
            8531-8534           AL079300.11        22895-22898
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q12.1"
     gene            1..8534
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /note="myosin XVIIIB"
                     /db_xref="GeneID:84700"
                     /db_xref="HGNC:HGNC:18150"
                     /db_xref="MIM:607295"
     exon            1..106
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            107..254
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..7919
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /note="isoform 2 is encoded by transcript variant 2;
                     myosin 18B; unconventional myosin-XVIIIb"
                     /codon_start=1
                     /product="unconventional myosin-XVIIIb isoform 2"
                     /protein_id="NP_115997.5"
                     /db_xref="CCDS:CCDS54507.1"
                     /db_xref="GeneID:84700"
                     /db_xref="HGNC:HGNC:18150"
                     /db_xref="MIM:607295"
                     /translation="MAISSRLALWEQKIREEDKSPPPSSPPPLFSVIPGGFIKQLVRG
                     TEKEAKEARQRKQLAVASPEREIPEISISQPNSKSSSGTRSGSQQISQDDQSSSPGSS
                     DILGKESEGSRSPDPEQMTSINGEKAQELGSSATPTKKTVPFKRGVRRGDVLLMVAKL
                     DPDSAKPEKTHPHDAPPCKTSPPATDTGKEKKGETSRTPCGSQASTEILAPKAEKTRT
                     GGLGDPGQGTVALKKGEEGQSIVGKGLGTPKTTELKEAEPQGKDRQGTRPQAQGPGEG
                     VRPGKAEKEGAEPTNTVEKGNVSKDVGSEGKHVRPQIPGRKWGGFLGRRSKWDGPQNK
                     KDKEGVLLSKAEKTGEPQTQMEKTSQVQGELGDDLRMGEKAGELRSTTGKAGESWDKK
                     EKMGQPQGKSGNAGEARSQTEKGCEAPKEVSTMVESPAAPGKGGWPGSRGQEAEEPCS
                     RAGDGAGALETELEGPSQPALEKDAERPRIRKENQDGPAPQEEGKGGQSRDSDQAPED
                     RWYEAEKVWLAQKDGFTLATVLKPDEGTADLPAGRVRLWIDADKTITEVDEEHVHRAN
                     PPELDQVEDLASLISVNESSVLNTLLQRYKAQLLHTCTGPDLIVLQPRGPSVPSAGKV
                     PKGRRDGLPAHIGSMAQRAYWALLNQRRDQSIVALGWSGAGKTTCCEQVLEHLVGMAG
                     SVDGRVSVEKIRATFTVLRAFGSVSMAHSRSATRFSMVMSLDFNATGRITAAQLQTML
                     LEKSRVARQPEGESNFLVFSQMLAGLDLDLRTELNLHQMADSSSFGMGVWSKPEDKQK
                     AAAAFAQLQGAMEMLGISESEQRAVWRVLAAIYHLGAAGACKVGRKQFMRFEWANYAA
                     EALGCEYEELNTATFKHHLRQIIQQMTFGPSRWGLEDEETSSGLKMTGVDCVEGMASG
                     LYQELFAAVVSLINRSFSSHHLSMASIMVVDSPGFQNPRHQGKDRAATFEELCHNYAH
                     ERLQLLFYQRTFVSTLQRYQEEGVPVQFDLPDPSPGTTVAVVDQNPSQVRLPAGGGAQ
                     DARGLFWVLDEEVHVEGSSDSVVLERLCAAFEKKGAGTEGSSALRTCEQPLQCEIFHQ
                     LGWDPVRYDLTGWLHRAKPNLSALDAPQVLHQSKREELRSLFQARAKLPPVCRAVAGL
                     EGTSQQALQRSRMVRRTFASSLAAVRRKAPCSQIKLQMDALTSMIKRSRLHFIHCLVP
                     NPVVESRSGQESPPPPQPGRDKPGAGGPLALDIPALRVQLAGFHILEALRLHRTGYAD
                     HMGLTRFRRQFQVLDAPLLKKLMSTSEGIDERKAVEELLETLDLEKKAVAVGHSQVFL
                     KAGVISRLEKQREKLVSQSIVLFQAACKGFLSRQEFKKLKIRRLAAQCIQKNVAVFLA
                     VKDWPWWQLLGSLQPLLSATIGTEQLRAKEEELTTLRRKLEKSEKLRNELRQNTDLLE
                     SKIADLTSDLADERFKGDVACQVLESERAERLQAFREVQELKSKHEQVQKKLGDVNKQ
                     LEEAQQKIQLNDLERNPTGGADEWQMRFDCAQMENEFLRKRLQQCEERLDSELTARKE
                     LEQKLGELQSAYDGAKKMAHQLKRKCHHLTCDLEDTCVLLENQQSRNHELEKKQKKFD
                     LQLAQALGESVFEKGLREKVTQENTSVRWELGQLQQQLKQKEQEASQLKQQVEMLQDH
                     KRELLGSPSLGENCVAGLKERLWKLESSALEQQKIQSQQENTIKQLEQLRQRFELEIE
                     RMKQMHQKDREDQEEELEDVRQSCQKRLHQLEMQLEQEYEEKQMVLHEKQDLEGLIGT
                     LCDQIGHRDFDVEKRLRRDLRRTHALLSDVQLLLGTMEDGKTSVSKEELEKVHSQLEQ
                     SEAKCEEALKTQKVLTADLESMHSELENMTRNKSLVDEQLYRLQFEKADLLKRIDEDQ
                     DDLNELMQKHKDLIAQSAADIGQIQELQLQLEEAKKEKHKLQEQLQVAQMRIEYLEQS
                     TVDRAIVSRQEAVICDLENKTEFQKVQIKRFEVLVIRLRDSLIKMGEELSQAATSESQ
                     QRESSQYYQRRLEELKADMEELVQREAEASRRCMELEKYVEELAAVRQTLQTDLETSI
                     RRIADLQAALEEVASSDSDTESVQTAVDCGSSGRKEMDNVSILSSQPEGSLQSWLSCT
                     LSLATDTMRTPSRQSATSSRILSPRINEEAGDTERTQSALALSRARSTNVHSKTSGDK
                     PVSPHFVRRQKYCHFGDGEVLAVQRKSTERLEPASSPLASRSTNTSPLSREKLPSPSA
                     ALSEFVEGLRRKRAQRGQGSTLGLEDWPTLPIYQTTGASTLRRGRAGSDEGNLSLRVG
                     AKSPLEIEGAAGGLLRSTSLKCISSDGVGGTTLLPEKSKTQFSSCESLLESRPSMGRK
                     LSSPTTPRDMLLSPTLRPRRRCLESSVDDAGCPDLGKEPLVFQNRQFAHLMEEPLGSD
                     PFSWKLPSLDYERKTKVDFDDFLPAIRKPQTPTSLAGSAKGGQDGSQRSSIHFETEEA
                     NRSFLSGIKTILKKSPEPKEDPAHLSDSSSSSGSIVSFKSADSIKSRPGIPRLAGDGG
                     ERTSPERREPGTGRKDDDVASIMKKYLQK"
     exon            255..413
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            414..1727
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1794
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1907
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..2084
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2283
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2426
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..2527
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2528..2591
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2736
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2737..2910
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            2911..3001
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3194
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3195..3275
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3276..3423
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3583
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3584..3767
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3768..3990
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            3991..4100
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4101..4166
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4167..4295
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4296..4439
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4440..4529
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4530..4649
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4650..4758
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4759..4883
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            4884..5038
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5039..5162
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5163..5363
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5364..5474
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5475..5579
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5580..5732
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5733..5846
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5847..5963
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            5964..6047
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            6048..6185
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            6186..6371
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            6372..6502
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            6503..6547
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            6548..6685
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            6686..7931
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     exon            7932..8534
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8111..8116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /note="hexamer: AATAAA"
     polyA_site      8133
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /note="major polyA site"
     regulatory      8405..8410
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /note="hexamer: ATTATA"
     polyA_site      8426
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
     regulatory      8510..8515
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /note="hexamer: AATAAA"
     polyA_site      8534
                     /gene="MYO18B"
                     /gene_synonym="KFS4"
                     /note="major polyA site"
ORIGIN      
        1 gctccttcgc tcctgctttc ccggctcggt ggggtccctt cgcggccacc cggggagacg
       61 tcttagacag tcgtggttcc tgtgatctgt gtcctctttg cagaagttct gtgtccatct
      121 catgtgctgc gtgtgtctgt aaagcctcat tccgtgctgt ctggcaggaa gctccatctc
      181 atctcatcat ctcacggccc tggcactgcc tcagcatggc catctcatca cgcctcgccc
      241 tgtgggagca gaagattcgg gaagaggaca agagccctcc accatcctcg ccccctcctc
      301 ttttctctgt catcccaggg ggcttcatta agcaactggt ccgggggact gaaaaagagg
      361 ccaaggaagc gagacagagg aagcagttag ctgtcgcctc tccagaacga gagatcccag
      421 aaatttccat cagccaaccc aacagcaagt ccagcagtgg caccagatct ggaagccagc
      481 agatctctca ggacgaccag tcaagctctc ctgggagctc agacattctg ggcaaggaga
      541 gcgaggggtc ccgcagcccc gaccctgagc agatgacaag catcaatggt gagaaggccc
      601 aggagctggg ctccagtgcg acaccaacca aaaagactgt ccccttcaag aggggcgtga
      661 ggaggggtga tgtgttgttg atggtggcca agctggaccc ggactcagcc aagccagaga
      721 agactcatcc ccatgacgcc cccccttgca agacctctcc ccccgccaca gatactggaa
      781 aggaaaagaa aggggagacc tctaggactc cttgtggctc ccaggccagc accgagatct
      841 tggccccgaa agctgagaag acccggactg ggggtcttgg ggacccaggc caaggaactg
      901 tggcactgaa aaaaggcgag gagggtcaaa gcatagtggg gaaggggctt gggaccccca
      961 agaccacaga gctgaaagag gctgagcccc agggcaaaga caggcagggg accaggcccc
     1021 aagcccaagg gcccggcgag ggggtgcgac cagggaaagc agagaaggag ggagcagagc
     1081 ccacaaacac ggtggaaaag gggaatgtct ctaaggacgt agggagtgaa gggaagcacg
     1141 taaggcccca aatccctggg agaaagtggg gaggtttcct gggaagaagg agtaagtggg
     1201 acggtcccca gaataagaag gacaaagaag gggtgctctt aagtaaggca gagaagacag
     1261 gtgagcctca gacccagatg gagaagacaa gccaagtgca gggcgagttg ggggacgatc
     1321 tgagaatggg ggagaaagca ggtgagcttc ggagcacgac tgggaaggca ggtgagtcct
     1381 gggataagaa ggaaaagatg gggcaacccc agggtaagtc cgggaacgca ggtgaagctc
     1441 ggagtcagac agagaagggc tgtgaagccc caaaggaggt gagcacaatg gtggagtcgc
     1501 cagcagctcc tgggaaggga ggctggccag gaagccgtgg gcaggaagca gaggagccct
     1561 gctcaagagc aggtgatggg gctggtgccc tggagacaga gctggaagga cccagccagc
     1621 ctgctctgga gaaggatgca gaaaggcctc ggatacggaa ggagaaccaa gacgggccag
     1681 ccccgcagga ggagggcaaa ggaggccaga gcagagactc agaccaggct cctgaggaca
     1741 gatggtatga ggcagagaaa gtctggctgg ctcagaagga tggatttact cttgctacgg
     1801 tgctaaagcc agatgaggga acagcagacc tgccagcagg aagggtgaga ctttggattg
     1861 atgctgacaa aaccatcact gaggtggatg aggagcatgt ccatcgggcc aaccctcctg
     1921 agctggacca ggtcgaggac ctggcctctc tcatcagtgt caacgaatcc agtgtcctga
     1981 acacgcttct gcagcgctac aaagctcagc tgctgcacac ctgcacaggg cctgatctga
     2041 ttgtcctcca gccccggggg ccctcggtgc cttctgcagg gaaggtgccc aagggccgcc
     2101 gggatggcct gcctgcccac attggctcca tggcacagcg ggcatactgg gcgctgctga
     2161 accagcggag agaccagagc attgtggccc tgggctggag tggcgctggg aagaccacct
     2221 gctgtgagca ggtcctggaa cacctggtgg ggatggcagg cagtgtggat ggcagggtct
     2281 cagtggagaa gatccgagcc accttcactg tcctccgggc cttcggctct gtgtccatgg
     2341 cccacagccg cagtgccacc cggttctcca tggtgatgtc gctggacttc aacgctacag
     2401 gccgcatcac agctgctcag ctccagacaa tgcttttgga gaagagccgc gtggcacggc
     2461 agccggaagg ggaaagtaac ttcctggttt tctcccagat gctggctgga ttggacttgg
     2521 atctcaggac ggagctgaac ctgcaccaga tggcagatag cagctccttt ggcatgggcg
     2581 tgtggtccaa gcctgaagat aaacagaagg cggcagctgc ctttgcccag ctccagggtg
     2641 ccatggagat gctcggcatc tcagagagcg agcagcgggc tgtttggcgg gtcctggcag
     2701 ccatctacca cctgggtgcg gcgggggcct gcaaagtggg tcggaagcag ttcatgaggt
     2761 ttgagtgggc aaactacgca gctgaggccc tgggctgcga gtatgaggag ctgaacacgg
     2821 ccaccttcaa gcaccacctt cgacagatca tccagcaaat gacgtttggg ccaagccgat
     2881 ggggcctcga ggatgaggaa accagctcag ggctcaagat gacaggagtg gactgtgtgg
     2941 aggggatggc ctcgggcctg taccaggaac tctttgcggc tgtggtctca ctcatcaaca
     3001 gatccttttc ctcccaccat ctctccatgg cctccatcat ggtggtggac tctccaggct
     3061 tccagaaccc ccggcaccag ggcaaggacc gggcggccac ctttgaggag ctgtgccaca
     3121 actacgccca tgagcgcctg cagctgctgt tctaccagcg gacctttgtc tccacgctac
     3181 agcgatatca agaggaaggt gttcctgtgc agtttgacct cccggacccc tccccaggga
     3241 ccaccgtggc tgttgtggat caaaatccct ctcaggtccg cttaccagct ggaggaggtg
     3301 cccaggatgc cagaggcctt ttctgggtct tagatgagga agtccatgta gagggctcca
     3361 gtgacagtgt ggtgctcgag cgtctgtgtg ctgctttcga gaagaaagga gctgggactg
     3421 aagggtcctc tgccctgcgg acctgtgagc agcccctcca gtgtgagatt ttccaccagt
     3481 tgggatggga ccctgtgcgg tacgacctca cgggctggct ccacagagcc aagcccaacc
     3541 tctcggccct ggatgcaccc caggtcctgc accagtcaaa aagagaggag ctgcggagtc
     3601 tattccaggc ccgggccaag ctgcctcctg tgtgccgggc tgtggcaggc ctggagggca
     3661 cctcccagca ggccctgcag aggagccgca tggtgaggag gacctttgcc agcagccttg
     3721 ccgcggtgag gaggaaagcc ccgtgctccc agatcaagct gcagatggat gcgctgacca
     3781 gcatgatcaa aaggtcccgg ctgcacttta tccactgcct ggtaccaaac cctgtggtgg
     3841 aaagcaggag tgggcaggaa tctccaccac caccgcagcc tggtagagac aagcctgggg
     3901 caggtggacc tctggccctg gatatcccag cactgagggt ccagcttgct gggttccaca
     3961 tcctggaggc tctgcgtctg cataggacag gctatgctga ccacatgggg ctcactcgct
     4021 tccgccggca attccaggtg ctggacgctc cactcctgaa gaagctcatg tcgacctccg
     4081 agggaataga tgaaaggaag gccgtggagg agctcctgga gaccctggat ctggaaaaga
     4141 aggcggtggc tgtggggcac agccaagttt ttctcaaggc aggtgtgatc tccaggcttg
     4201 agaagcagcg agagaagctg gtatctcaga gcatcgttct cttccaggcg gcttgcaagg
     4261 gctttctgtc tcgccaggaa ttcaagaagc tgaagattcg ccgactggct gcacagtgca
     4321 tccagaagaa tgtggctgtg ttcctcgcag tcaaggactg gccatggtgg cagctgcttg
     4381 gttccctcca gcctctactt agtgccacca ttggaactga gcagctccga gccaaggagg
     4441 aggagcttac aacgctaaga cggaagctag aaaaatcaga gaagttgcgg aatgaactcc
     4501 ggcagaacac agatctgcta gaaagcaaga ttgctgactt gacctctgac cttgccgatg
     4561 agcgcttcaa aggtgatgtg gcctgccagg tgctggagag tgagcgggca gagcggctac
     4621 aggccttccg ggaggtccag gagctcaaga gcaagcatga acaagtccag aaaaaactgg
     4681 gagatgtgaa taaacagttg gaagaagccc agcagaaaat tcagttgaat gacttggaaa
     4741 ggaatcccac tggaggagca gacgagtggc agatgcgctt cgactgtgct cagatggaga
     4801 acgagttcct cagaaagcgt ctgcagcaat gcgaggagag gctggactcg gagctgacag
     4861 ccaggaaaga gctggagcaa aagcttgggg agttgcaaag tgcttatgac ggggccaaga
     4921 agatggctca ccaactgaag aggaagtgcc accatcttac ctgtgacctt gaggatacct
     4981 gcgtcctgct agagaaccaa caaagtcgaa accatgagct ggagaagaag cagaagaagt
     5041 ttgacctgca gctggcccag gccctaggtg agtcagtgtt tgagaagggt ctccgtgaga
     5101 aagtgaccca ggagaacacc agtgtccggt gggagctagg ccagcttcag cagcagctga
     5161 agcaaaagga gcaggaagcc tcacagctga agcagcaggt ggagatgcta caggaccata
     5221 aacgggagct gctggggtca ccctctctgg gggaaaattg cgttgctggc ttgaaggaga
     5281 ggctctggaa gttggaatcc agcgcccttg agcaacagaa aatccagagc cagcaggaaa
     5341 acaccatcaa gcagctggag cagctccgcc agcggtttga gctggagatc gagcggatga
     5401 agcagatgca ccagaaggac cgtgaggacc aggaggagga actggaggat gtccgtcagt
     5461 cctgccagaa gcggcttcat cagctggaaa tgcagctgga gcaagagtat gaagagaagc
     5521 agatggtcct ccatgagaag caagatttgg aaggcttgat cggaaccctc tgtgaccaga
     5581 ttggccatcg ggactttgat gtggagaagc gacttcggag agacctcagg aggacacatg
     5641 cactgttgtc agacgtgcag ctccttctgg gcaccatgga ggatggcaag acatcagtca
     5701 gcaaggagga gctggagaaa gtgcacagcc agctggagca gagtgaagcc aagtgtgagg
     5761 aggccttgaa gacgcagaag gtgctcacag cggacctgga gagcatgcac agcgagctgg
     5821 agaacatgac gcggaacaag agcctggtgg atgagcagct gtacaggctg cagtttgaga
     5881 aggcggacct cctgaagcgc atcgatgagg accaggatga cctgaatgag ctgatgcaga
     5941 agcacaagga cctcattgct cagtctgctg ctgacattgg gcagatccaa gaactgcagc
     6001 tgcagctgga ggaagccaag aaggagaagc acaagctaca agaacaactg caggtggctc
     6061 agatgcgcat cgagtacctg gaacagtcca ccgtggatcg agccatcgtc agcaggcagg
     6121 aggcggtcat ctgtgaccta gagaacaaga cagagttcca gaaggtgcag attaagagat
     6181 ttgaggtcct ggtgatccgg cttcgggaca gcctgatcaa gatgggggag gagctttcac
     6241 aggcggccac ctccgagtcc cagcagcggg agagcagcca gtactaccag cggcgcctgg
     6301 aagagctgaa ggccgacatg gaagagctgg tgcagcggga ggcagaggcc agccggcggt
     6361 gcatggagct ggagaagtac gtggaggaac ttgcagcagt gaggcaaacc ctccagacag
     6421 acctggagac atccattcgg cggattgccg acctgcaggc tgccttggaa gaagtggcat
     6481 ccagtgacag tgatactgag agtgtccaga cggcagtgga ttgtggcagc agcggccgaa
     6541 aagagatgga taacgtctcc atcctcagct cccagccaga gggcagcctg cagtcctggt
     6601 tgagctgtac tctgtccctg gccacagata ctatgaggac tccttctcga cagtcagcca
     6661 ccagcagccg catcctcagc cccaggataa acgaagaggc tggggacact gagaggaccc
     6721 agtcggcatt ggcactgagc agagcccggt ccaccaatgt ccacagcaag acctcaggag
     6781 acaagcctgt ttctccccac tttgtccgcc ggcaaaagta ctgtcatttt ggggacggcg
     6841 aagtgcttgc cgtccagaga aagtccacag agagattaga acctgcttcc tctcccctgg
     6901 cttctcggag tacaaataca tccccgctgt cgagggaaaa gctgcccagt ccttcagcgg
     6961 ccctctcgga gttcgtggaa gggctccgga ggaagagagc ccagagaggc caggggtcca
     7021 cgctgggcct agaggactgg cccactctcc ccatttacca gacgactggg gcctccacac
     7081 taaggagggg cagggctggc agtgacgagg gaaacctctc gctgagggtt ggggcaaagt
     7141 cacccctgga aatcgaaggg gccgctggtg gtctcttgag gtccaccagc ctcaaatgca
     7201 tctcttcaga cggtgttggg ggcacaaccc tactccccga aaagtcgaaa acccaattca
     7261 gttcctgcga gtccctctta gaatccagac cgagcatggg gagaaaactg agctctccga
     7321 ccacacccag ggacatgctg ttgtcgccca cactgcgtcc tcggaggcgg tgtctggagt
     7381 cctctgtgga cgatgcgggc tgtccagacc ttggaaagga gccgcttgtt ttccagaacc
     7441 gccagtttgc ccacctgatg gaggaacctc taggcagtga cccattcagc tggaaactcc
     7501 caagcctcga ctacgaacgc aagaccaaag tggacttcga tgacttcctc ccagctatcc
     7561 ggaagcccca gacacctacc tccttggctg gatcagccaa aggtgggcaa gacggttcac
     7621 agcgttcaag catccacttt gaaacggaag aggctaaccg ttcctttctc tcggggatca
     7681 agaccatttt gaagaagagc ccggagccca aggaggatcc cgctcacctg tctgactcgt
     7741 cctcatcctc cggctccatc gtgtccttca aaagtgctga cagcatcaaa agtcgaccag
     7801 gaatcccacg acttgcgggt gacggtggcg agcgaacgtc ccccgagcgg agagagccag
     7861 ggacggggag gaaagacgac gatgttgcga gcataatgaa gaaatacctc cagaagtagg
     7921 aaccagttca gcctccttga agctgccctt gaagacttcc cgactctaca ataacttgga
     7981 gacagagaga ctggccaggc ctccccggtg gccagagcca gccagcatgg ccaccctcaa
     8041 gaggcgagat gagcccacag aggcatatcc tgcggggatg ctgggctccc agtgtggttg
     8101 gcctgaacaa aataaagtgt tgactcctgg gcatctgtgc cttctctatg gccttgctac
     8161 ctgggattcc agagagttga tggggtgcag ataggggtag gactgttaga atagaaccaa
     8221 cccaaactgt gtgtagtttg gggtgtatac ttctatttct cttcctacat gtctacatgc
     8281 catgaccttc ctcctcctct tcacttggcc agtttcagct cacttcctcc aggaagtctt
     8341 tcctgatata tcaaactgaa acaaatgctc ctcctccatg ctcccttaat ccccatgctt
     8401 gtcgattata ttcctttgcc aattcatttc tctatcctgt gtatgtataa gtgtgtacaa
     8461 gcattcaaga aactgatgaa tgatgaatga atgaatgagc caaagaacaa ataaatgagc
     8521 ccacacaccc tgaa
//
