LOCUS       NM_001012418            5754 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens myosin light chain kinase family member 4 (MYLK4),
            transcript variant 1, mRNA.
ACCESSION   NM_001012418 XM_373109
VERSION     NM_001012418.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   Yang,M., Zhang,T., Zhang,Y., Ma,X., Han,J., Zeng,K., Jiang,Y.,
            Wang,Z., Wang,Z., Xu,J., Hua,Y., Cai,Z. and Sun,W.
  TITLE     MYLK4 promotes tumor progression through the activation of
            epidermal growth factor receptor signaling in osteosarcoma
  JOURNAL   J Exp Clin Cancer Res 40 (1), 166 (2021)
   PUBMED   33980265
  REMARK    GeneRIF: MYLK4 promotes tumor progression through the activation of
            epidermal growth factor receptor signaling in osteosarcoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5754)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   4  (bases 1 to 5754)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 5754)
  AUTHORS   Hong,K.W., Kim,S.S. and Kim,Y.
  TITLE     Genome-wide association study of orthostatic hypotension and
            supine-standing blood pressure changes in two korean populations
  JOURNAL   Genomics Inform 11 (3), 129-134 (2013)
   PUBMED   24124408
REFERENCE   6  (bases 1 to 5754)
  AUTHORS   Taipale,M., Krykbaeva,I., Koeva,M., Kayatekin,C., Westover,K.D.,
            Karras,G.I. and Lindquist,S.
  TITLE     Quantitative analysis of HSP90-client interactions reveals
            principles of substrate recognition
  JOURNAL   Cell 150 (5), 987-1001 (2012)
   PUBMED   22939624
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK096893.1, BX647582.1, AK122581.1, AL138876.23 and BU675945.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_001012418.4.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the shorter isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because transcript sequence consistent with
            the reference genome assembly was not available for all regions of
            the RefSeq transcript. The extent of this transcript is supported
            by transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647582.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000274643.9/ ENSP00000274643.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AK096893.1         45-46
            3-1410              BX647582.1         1-1408
            1411-1661           AK122581.1         413-663
            1662-5250           AL138876.23        75940-79528         c
            5251-5754           BU675945.1         26-529              c
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p25.2"
     gene            1..5754
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /note="myosin light chain kinase family member 4"
                     /db_xref="GeneID:340156"
                     /db_xref="HGNC:HGNC:27972"
     exon            1..187
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            188..458
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     CDS             300..1466
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     caMLCK like; sugen kinase 85"
                     /codon_start=1
                     /product="myosin light chain kinase family member 4
                     isoform 1"
                     /protein_id="NP_001012418.2"
                     /db_xref="CCDS:CCDS34330.1"
                     /db_xref="GeneID:340156"
                     /db_xref="HGNC:HGNC:27972"
                     /translation="MLKVKRLEEFNTCYNSNQLEKMAFFQCREEVEKVKCFLEKNSGD
                     QDSRSGHNEAKEVWSNADLTERMPVKSKRTSALAVDIPAPPAPFDHRIVTAKQGAVNS
                     FYTVSKTEILGGGRFGQVHKCEETATGLKLAAKIIKTRGMKDKEEVKNEISVMNQLDH
                     ANLIQLYDAFESKNDIVLVMEYVDGGELFDRIIDESYNLTELDTILFMKQICEGIRHM
                     HQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVN
                     YDFVSFPTDMWSVGVIAYMLLSGLSPFLGDNDAETLNNILACRWDLEDEEFQDISEEA
                     KEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK"
     exon            459..534
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            535..640
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            641..734
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            735..844
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            845..986
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1057
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1186
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1339
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1418
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1491
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     exon            1492..5754
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5734..5739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /note="hexamer: AATAAA"
     polyA_site      5754
                     /gene="MYLK4"
                     /gene_synonym="SgK085"
                     /note="major polyA site"
ORIGIN      
        1 tgagagagaa gatacttcct ctcgtagtgg acacttgtct tttcagaaga tttatacgac
       61 tgagagtcaa aacttttacc agagactttc atagtcagtt tgaattctgc aaaatttctc
      121 tttatcttct ggaatgtatg ctctagactt ggagtggctc aagctcttcg atgtgatcct
      181 atcaatgtca agagaaagaa gcactgaaga gtcataattg tgtcctgatt tataaatcgg
      241 agacagaggg agacgaaaac cacactccag aaagtagctt aatcggactc actactaaga
      301 tgttaaaagt gaagaggctg gaagaattca acacgtgtta taacagcaac cagctggaga
      361 aaatggcctt ttttcagtgc agggaagagg tggagaaagt gaagtgtttt ctggaaaaaa
      421 attctgggga ccaggattca agatctggac ataatgaggc gaaggaggtg tggtcaaacg
      481 ccgacctgac ggaaaggatg cccgtcaaaa gcaaaaggac atcagccctc gcagttgaca
      541 tcccggctcc tccggcccca tttgatcatc gtattgtgac agccaagcaa ggagcggtca
      601 acagcttcta tactgtgagc aagacagaaa tcctaggagg agggcgtttc ggccaggttc
      661 acaagtgtga ggagacggcc acaggtctga agctggcagc caaaatcatc aagaccagag
      721 gcatgaagga caaggaggag gtgaagaacg agatcagcgt catgaaccag ctggaccacg
      781 cgaacctcat ccagctgtac gatgccttcg agtctaagaa cgacattgtc ctggtcatgg
      841 agtatgtgga tggtggggag ctgtttgacc gcatcatcga tgagagctac aatttgacgg
      901 agcttgatac catcctgttc atgaagcaga tatgtgaggg gataaggcac atgcatcaga
      961 tgtacattct ccacttggac ctgaagcctg agaatatcct gtgtgtgaat cgggatgcta
     1021 agcaaataaa aattattgat tttggattgg ccagaagata caaacccaga gagaagctga
     1081 aggtgaactt tggaacccca gaatttctcg cccctgaagt tgtgaactat gattttgttt
     1141 catttcccac tgacatgtgg agtgtggggg tcatcgccta tatgctactt agcggtttgt
     1201 cgcctttcct gggtgacaat gatgctgaga cgctgaacaa catcctggcc tgcaggtggg
     1261 acttagagga tgaagaattt caggacatct cggaggaggc caaggagttc atctctaagc
     1321 ttctgattaa ggagaagagt tggcgaataa gtgcaagcga agctctcaag cacccctggt
     1381 tgtcagacca caagctccac tccagactca atgcccagaa gaagaagaat cgtggctctg
     1441 atgcccagga ctttgtgacc aaatagtcta caggaggcag ccatttggaa ggaaaactgc
     1501 tgtggttgct gctgcttcga gaaaattttt tgaaaaatca gcagttctga tgccttgacc
     1561 cctgtgatga cctggtagtc ttagcagggg gagccctcga ccctgaatgt gaacttgaac
     1621 tggagtgcct ctgctgcgct cagaggaaca cccagcgctg cggtctggtc tcagggcgca
     1681 aacacatccc tgcacccggt ggtggtgatg ttgggaagat gtttccctgc catcttgaga
     1741 ttttttactt ttttaaaaaa attttttgat cttagtcact ggggcagggg gtgcattatg
     1801 tctgccattt ctcaaatgac tacagctgtg acacttaaga ttgattggag attttaaaac
     1861 tggaggatgc atttagaggg agaaagctac ttccagccat tagactatgc ctttggaagg
     1921 ggcaattaga tggccagaaa gaattctgac tcccatgcac ccttggagac ttagtttcac
     1981 agactctgtc attttgattt ggtctgtggt cacgtgacta caatggataa ataatttgtt
     2041 tgctggaaca gaagccgtct ttgaattctc caacaagagg catcagccac tgtgtctttc
     2101 atttattctg aattgtcccc agcgtgcgtt ttcttccatg tagctgattc tgtttttcac
     2161 atctatactc cctgcaccct tctgactgac agttcatccc cacaaaacac attggttttg
     2221 agaggtgatg caaaaagtat ctgtttatgg ttggactttg aaattctgac ctttgaaata
     2281 atgtcaacag tcacaacagg tttcctcaag ccccatttgt caactaaaag agacggatct
     2341 gcacctcctg gagcaagata gcactgagct ttcaacattg aagtgacttg ggaaataaca
     2401 ttcaagcttc tgggtaaaca catacaaaca gtggcaaaga ccaagtacag tctctcgcct
     2461 gtgctgtctc atgggactca atggtggtga agaaatctca atcggacacc agaggtgggg
     2521 gcaactctgc aagctcacct gctggacgtg agaatgttgg aacgttccag ttggaaggtt
     2581 cctatgccta gcctgtgtgc catttcatgg cagatatgta agaaaggaaa tggccctact
     2641 gtagacagct tatggggccc agaaaggcac tgggctttaa agagcgaaat gagtgtgtca
     2701 tttccacatg aaggagaggc aaggaaaagc cccacaaaat tcctgttacc cgctgaactc
     2761 caagctcacc aaccagattc atgagctttc tcacattttc catttccaaa taactgttgt
     2821 gttgaatcag ctgtgttaat gcctttattc tttgtttttt ttttttagac ggagtctcgc
     2881 tctgtcacga ggctggagtg cagtggcccc atctcggctc actgcaacct ccgcctccca
     2941 ggttcaagcg attctcctgc agcctcccga gtagctggga ttacaggcgc acgccaccac
     3001 gcacggctga tttttgcatt tttagtagag atggggtttc tccatgttgg ccagaatggt
     3061 ctcaattact gttgtcgcaa tagcaatgaa aggcataaga attacttggt ggcctcatga
     3121 gggatggcag tttcactttg catcagcttt cccccattat ctctcagatc cacttaagga
     3181 atccttacag catgccgtga atgtgtgcaa catccttatt ctcatcttta aggtaaggag
     3241 gaagtagaga tagaaggaaa tacgtgattt ggtgaaggcc acatgcgaat tttgtggtcg
     3301 tgttagttat aaaactgact cctttccagc ctttgctctt acattatgat gcgtttcttt
     3361 ttctcctttg gccttgcatt tatccgaggt tggtttctta tgttcctgtg atgtgactat
     3421 gcttaagtgt ttaatttagt ttgcttaaaa cacacacacc acacacacac acacacacaa
     3481 aaccctgtga ctggagtgtt atattactat gctatcttta gctgatgata ttcattgagc
     3541 ctggaaaaat acaagccagt gcatgcatta atacttagtt agaccgcaga gagctggcat
     3601 tcactcaaat gctaaacagg acacaggggt ctgatttgaa gcccctcgtg gcagggcgtt
     3661 ttctgtcctg tggtgtgcat tctctaccaa atcaagcagg agatcttttg cacgacgtgc
     3721 aatttgcatc caccttccct tctgactcag gttggcagat aaactgtccc cagaccctgc
     3781 cacacatgga agagacagct caggccaaat acaggcatga tttctttgcc aagttaatgc
     3841 actgatgacc ctgcagtgag tgggtgtgat ttttgttttg ttttgaggtt tagaaaggag
     3901 actgtacttt gaaaatggat cttgtttgat tctgtagtta aatttccaca tttggaatgg
     3961 gaaggcatct ccatgccagc agggtgcctt ttatatgagt gctgagggta gtttgcattg
     4021 cagagttcat gattacagtc tagagttact tgaaatggga gattatcagt ctagtgttca
     4081 tcccgcccct gctccatgtc cttgtgtggc tgctccagcg agagctggta actaggtaga
     4141 ctgctgctct aatggggaaa gggggctttg caaagccgag gaatcaagcc agatgcgagc
     4201 atggactcag aaaagctgag tctggctaga ccagggctat ggagatcaat ttttccaaca
     4261 ggtcattagg atttgggagg ctgtatttct cctccaccct cttaatggat ggtatggctt
     4321 tgctcactca ctaggcagtt cccactttct ttagcaaatg gaggaaatta agcaccgatg
     4381 tagcacttcc cactgcctgg agagaaataa acctcgcaag ttattaagga gaaggcatgc
     4441 ctcattggta atttggaaga caccaacact tacccagaat ctgttgctat ttaaaaatca
     4501 gggagaggag ggcacatgtc cccatttgct acattacaaa ctttggacga gactctcatc
     4561 tgaactaact tggggaaaca atttctaggt tgaagctggt aacactcatg tctgttgagt
     4621 gccataagtt tgttttcagt tatttcctct aacttgatat taatttgctt tctttcctca
     4681 aaaatgtaag taggcatgtt gccattgttg tggcttgctt tgatgtatgt ttggaaagat
     4741 gtgtgaatac ttaattttta tccattttat ggaaattagc ttagcaattt ttttttctct
     4801 tttaagaatc aaaatatgga tttgatacta ggaacagaat ctgctcttta tttcattcct
     4861 tttttgtctc ttctgcttcc tctgcccctc ccccagccac gcagcacggg tgccagtgtg
     4921 catgcgtgtg ctgggggcca aatgacagaa ccccggttga aagtccgctt gtaaaagcac
     4981 aagtgagagg cagcatcagg cccctggagg gctgtggtcc tcggactcgg ttctttcctg
     5041 tcccctggcc gtcctgtcat ccctgtgtgc tgcagcatca ccacggccta cttcccaata
     5101 tccacaaagc ttcgcctttg cttttgcact ctcttttaca tttttttact tctttccttt
     5161 ttcttaagtt cggatccaag tttgtcttct tatccatcac catgctccct gaaagccttt
     5221 ttgagattaa gaaattttct taccaggagg caactttccg tctgtcctaa agggaagtgg
     5281 gtgctggatt tattagatat tgggttcctg gattaagatg acttcctcac atagcttgga
     5341 gtctgcagtt tctcagttag aagtgttcta tatttctttt tgctccattt tagtgcgctg
     5401 tgctttgtat aagtggtctt tctatgggca gtttcacctt ccatgaaggc ttataactca
     5461 accactacta cccattcaga agagcagaaa gcatctgctg tgtgagcgtt catatgttac
     5521 ctaatgtgta ttttgctgtc gtctcagctg atagatgtca gtgagtcttt ctgactaggt
     5581 ttattggtgt tgataagaaa ttaaaaaaag gattcataac gtggtgattg tgtctatcat
     5641 gtatatttta aatttctgaa cgtactttca tttagctgtt tttctttgct ttctaaagat
     5701 gtataacaat catgtatatg tattatattg aagaataaac atggacctta ttta
//
