LOCUS       NM_182493               8033 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens myosin light chain kinase 3 (MYLK3), transcript
            variant 1, mRNA.
ACCESSION   NM_182493
VERSION     NM_182493.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Tobita,T., Nomura,S., Morita,H., Ko,T., Fujita,T., Toko,H., Uto,K.,
            Hagiwara,N., Aburatani,H. and Komuro,I.
  TITLE     Identification of MYLK3 mutations in familial dilated
            cardiomyopathy
  JOURNAL   Sci Rep 7 (1), 17495 (2017)
   PUBMED   29235529
  REMARK    GeneRIF: Study identified MYLK3 mutations associated with familial
            dilated cardiomyopathy. In vitro experiments and
            immunohistochemistry suggested that the MYLK3 mutations identified
            in this study result in markedly reduced levels of protein
            expression and myosin light chain 2 phosphorylation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8033)
  AUTHORS   Samak,G., Gangwar,R., Crosby,L.M., Desai,L.P., Wilhelm,K.,
            Waters,C.M. and Rao,R.
  TITLE     Cyclic stretch disrupts apical junctional complexes in Caco-2 cell
            monolayers by a JNK-2-, c-Src-, and MLCK-dependent mechanism
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 306 (11), G947-G958 (2014)
   PUBMED   24722904
  REMARK    GeneRIF: This study demonstrates that cyclic stretch disrupts tight
            junctions and adherens junctions by a JNK2, c-Src, and
            MLCK-dependent mechanism.
REFERENCE   4  (bases 1 to 8033)
  AUTHORS   Kuo,C.Y., Chou,T.Y., Chen,C.M., Tsai,Y.F., Hwang,G.Y. and
            Hwang,T.L.
  TITLE     Hepatitis B virus X protein disrupts stress fiber formation and
            triggers apoptosis
  JOURNAL   Virus Res 175 (1), 20-29 (2013)
   PUBMED   23591626
  REMARK    GeneRIF: These findings suggest that hepatitis B virus X protein
            disrupts stress fiber formation and triggers apoptosis via an MLCK
            and a PTEN-dependent pathway.
REFERENCE   5  (bases 1 to 8033)
  AUTHORS   Tsukamoto,O. and Kitakaze,M.
  TITLE     Biochemical and physiological regulation of cardiac myocyte
            contraction by cardiac-specific myosin light chain kinase
  JOURNAL   Circ J 77 (9), 2218-2225 (2013)
   PUBMED   23863751
  REMARK    GeneRIF: Biochemical and physiological regulation of cardiac
            myocyte contraction by cardiac-specific myosin light chain kinase.
            Review article
REFERENCE   6  (bases 1 to 8033)
  AUTHORS   Al-Sadi,R., Ye,D., Dokladny,K. and Ma,T.Y.
  TITLE     Mechanism of IL-1beta-induced increase in intestinal epithelial
            tight junction permeability
  JOURNAL   J Immunol 180 (8), 5653-5661 (2008)
   PUBMED   18390750
  REMARK    GeneRIF: data indicate that the IL-1beta-induced increase in MLCK
            protein expression and Caco-2 TJ permeability was mediated by an
            increase in MLCK expression and activity and by an
            NF-kappaB-dependent increase in MLCK gene transcription
REFERENCE   7  (bases 1 to 8033)
  AUTHORS   Chan,J.Y., Takeda,M., Briggs,L.E., Graham,M.L., Lu,J.T.,
            Horikoshi,N., Weinberg,E.O., Aoki,H., Sato,N., Chien,K.R. and
            Kasahara,H.
  TITLE     Identification of cardiac-specific myosin light chain kinase
  JOURNAL   Circ Res 102 (5), 571-580 (2008)
   PUBMED   18202317
REFERENCE   8  (bases 1 to 8033)
  AUTHORS   Seguchi,O., Takashima,S., Yamazaki,S., Asakura,M., Asano,Y.,
            Shintani,Y., Wakeno,M., Minamino,T., Kondo,H., Furukawa,H.,
            Nakamaru,K., Naito,A., Takahashi,T., Ohtsuka,T., Kawakami,K.,
            Isomura,T., Kitamura,S., Tomoike,H., Mochizuki,N. and Kitakaze,M.
  TITLE     A cardiac myosin light chain kinase regulates sarcomere assembly in
            the vertebrate heart
  JOURNAL   J Clin Invest 117 (10), 2812-2824 (2007)
   PUBMED   17885681
  REMARK    GeneRIF: Integrated cDNA expression analysis of failing human
            myocardia uncovered a novel protein kinase, cardiac-specific myosin
            light chain kinase (cardiac-MLCK), which acts on MLC2v.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC007225.2, AJ247087.1, DA561361.1, CR739116.1 and AL691746.1.
            
            On Nov 22, 2018 this sequence version replaced NM_182493.2.
            
            Summary: Phosphorylation of cardiac myosin heavy chains (see MYH7B,
            MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase,
            such as MYLK3, potentiates the force and rate of cross-bridge
            recruitment in cardiac myocytes (Chan et al., 2008 [PubMed
            18202317]).[supplied by OMIM, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ247087.1, BC109096.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968968 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394809.9/ ENSP00000378288.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC007225.2         137167-137201
            36-688              AJ247087.1         1-653
            689-1136            DA561361.1         122-569
            1137-2703           AJ247087.1         1102-2668
            2704-3181           CR739116.1         83-560
            3182-7915           AC007225.2         178378-183111
            7916-8033           AL691746.1         27-144              c
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q11.2"
     gene            1..8033
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /note="myosin light chain kinase 3"
                     /db_xref="GeneID:91807"
                     /db_xref="HGNC:HGNC:29826"
                     /db_xref="MIM:612147"
     exon            1..628
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..2611
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /EC_number="2.7.11.18"
                     /note="isoform 1 is encoded by transcript variant 1; MLC
                     kinase; cardiac-MyBP-C associated Ca/CaM kinase; putative
                     myosin light chain kinase 3; cardiac-MLCK"
                     /codon_start=1
                     /product="myosin light chain kinase 3 isoform 1"
                     /protein_id="NP_872299.2"
                     /db_xref="CCDS:CCDS10723.2"
                     /db_xref="GeneID:91807"
                     /db_xref="HGNC:HGNC:29826"
                     /db_xref="MIM:612147"
                     /translation="MSGTSKESLGHGGLPGLGKTCLTTMDTKLNMLNEKVDQLLHFQE
                     DVTEKLQSMCRDMGHLERGLHRLEASRAPGPGGADGVPHIDTQAGWPEVLELVRAMQQ
                     DAAQHGARLEALFRMVAAVDRAIALVGATFQKSKVADFLMQGRVPWRRGSPGDSPEEN
                     KERVEEEGGKPKHVLSTSGVQSDAREPGEESQKADVLEGTAERLPPIRASGLGADPAQ
                     AVVSPGQGDGVPGPAQAFPGHLPLPTKVEAKAPETPSENLRTGLELAPAPGRVNVVSP
                     SLEVAPGAGQGASSSRPDPEPLEEGTRLTPGPGPQCPGPPGLPAQARATHSGGETPPR
                     ISIHIQEMDTPGEMLMTGRGSLGPTLTTEAPAAAQPGKQGPPGTGRCLQAPGTEPGEQ
                     TPEGARELSPLQESSSPGGVKAEEEQRAGAEPGTRPSLARSDDNDHEVGALGLQQGKS
                     PGAGNPEPEQDCAARAPVRAEAVRRMPPGAEAGSVVLDDSPAPPAPFEHRVVSVKETS
                     ISAGYEVCQHEVLGGGRFGQVHRCTEKSTGLPLAAKIIKVKSAKDREDVKNEINIMNQ
                     LSHVNLIQLYDAFESKHSCTLVMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGV
                     HYLHQHYILHLDLKPENILCVNQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPE
                     VVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFEGLS
                     EEAKDFVSRLLVKEKSCRMSATQCLKHEWLNNLPAKASRSKTRLKSQLLLQKYIAQRK
                     WKKHFYVVTAANRLRKFPTSP"
     exon            629..719
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            720..1152
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1613
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..1719
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1720..1813
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..1923
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1924..2065
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2136
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2265
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2418
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2419..2551
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..8033
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6915..6920
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /note="hexamer: AATAAA"
     polyA_site      6934
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /note="major polyA site"
     regulatory      8007..8012
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /note="hexamer: AATAAA"
     polyA_site      8033
                     /gene="MYLK3"
                     /gene_synonym="caMLCK; MLCK; MLCK2"
                     /note="major polyA site"
ORIGIN      
        1 acacagccca ggccatcgtg gaggttggtg ctgccaggag tctgtcagct acggaggaca
       61 atgaccttgc agacaccacc gcctgagtga gaaccagggg tctgtgcctc tcctcattcc
      121 ccgctcttgc ccttgtcaag cctgcaccag catgtcagga acctccaagg agagtctggg
      181 gcatgggggg ctgccagggt tgggcaagac ctgcttaaca accatggaca caaagctgaa
      241 catgctgaac gagaaggtgg accagctcct gcacttccaa gaagatgtca cagagaagtt
      301 gcagagcatg tgccgagaca tgggccacct ggagcggggc ctgcacaggc tggaggcctc
      361 ccgggcaccg ggcccgggcg gggctgatgg ggttccccac attgacaccc aggctgggtg
      421 gcccgaggtc ctggagctgg tgagggccat gcagcaggat gcggcccagc acggtgccag
      481 gctggaggcc ctcttcagga tggtggctgc ggtggacagg gccatcgctt tggtgggggc
      541 cacgttccag aaatcaaagg tggcggattt cctcatgcag gggcgtgtgc cctggaggag
      601 aggcagccca ggtgacagcc ctgaggagaa taaagagcga gtggaagaag agggaggaaa
      661 accaaagcat gtgctgagca ccagtggggt gcagtctgat gccagggagc ctggggaaga
      721 gagccagaag gcggacgtgc tggaggggac agcggagagg ctgcccccca tcagagcgtc
      781 agggctggga gctgaccccg cccaggcagt ggtctcaccg ggccagggag atggtgttcc
      841 tggcccagcc caggcattcc ctggccacct gcccctgccc acaaaggtgg aagccaaggc
      901 tcctgagaca cccagcgaga acctcaggac tggcctggaa ttggctccag cacccggcag
      961 ggtcaatgtg gtctccccga gcctggaggt tgcaccaggt gcaggacaag gagcatcgtc
     1021 cagcaggcct gaccctgagc ccttagagga aggcacgagg ctgactccag ggcctggccc
     1081 tcagtgccca gggcctccag ggctgccagc ccaggccagg gcaacccaca gtggtggaga
     1141 aacacctcca aggatctcca tccacataca agagatggat actcctgggg agatgctgat
     1201 gacaggcagg ggcagccttg gacccaccct caccacagag gctccagcag ctgcccagcc
     1261 aggcaagcag ggcccacctg ggaccgggcg ctgcctccaa gcccctggga ctgagcccgg
     1321 agaacagacc cctgaaggag ccagagagct ctccccgctg caggagagca gcagccccgg
     1381 gggagtgaag gcagaggagg agcaaagggc tggggccgag cctggcacga gaccaagctt
     1441 ggccaggagt gacgacaatg accacgaggt tggggccctg ggcctgcagc agggcaaaag
     1501 cccaggggcg ggaaaccctg agcctgagca ggactgtgca gccagggctc cggtgagagc
     1561 tgaagcagta aggaggatgc ccccaggcgc cgaggctggc agcgtggttc tggatgacag
     1621 tccggcccca ccagctcctt ttgaacaccg ggtagtgagc gtcaaggaga cctccatctc
     1681 tgcgggttac gaggtgtgcc agcacgaagt cttgggaggg ggtcggtttg gccaggtcca
     1741 caggtgcaca gagaagtcca caggcctccc actggctgcc aagatcatca aagtgaagag
     1801 cgccaaggac cgggaggacg tgaagaacga gatcaacatc atgaaccagc tcagccacgt
     1861 gaacctgatc cagctctatg acgccttcga gagcaagcac agctgcaccc ttgtcatgga
     1921 gtacgtggac gggggtgagc tcttcgaccg gatcacagat gagaagtacc acctgactga
     1981 gctggatgtg gtcctgttca ccaggcagat ctgtgagggt gtgcattacc tgcaccagca
     2041 ctacatcctg cacctggacc tcaagccgga gaacatattg tgcgtcaatc agacaggaca
     2101 tcaaattaag atcattgact ttgggctggc cagaaggtac aagcctcgag agaagctgaa
     2161 ggtgaacttc ggcactcctg agttcctggc cccagaagtc gtcaattatg agtttgtctc
     2221 attccccaca gacatgtgga gtgtgggagt catcacctac atgctactca gtggcttgtc
     2281 cccatttcta ggggaaacag atgcagagac catgaatttc attgtaaact gtagctggga
     2341 ttttgatgct gacacctttg aagggctctc ggaggaggcc aaggactttg tttcccggtt
     2401 gctggtcaaa gagaagagct gcagaatgag tgccacacag tgcctgaaac acgagtggct
     2461 gaataatttg cctgccaaag cttcaagatc caaaactcgt ctcaaatccc aactactgct
     2521 gcagaaatac atagctcaaa gaaaatggaa gaaacatttc tatgtggtga ctgctgccaa
     2581 caggttaagg aaatttccaa cttctcccta atcttcaact ctgctgctcc aatgggtcca
     2641 gaaattactg aggccagtgg tgaagtgaag agatgactca aacatttaaa taatttggct
     2701 ttttggtatt attgattcca cttattttgt aaaaatggtt atggctgctg ccttccttgt
     2761 ggatgaaaag tggctgtaaa gaagcttcct aagaacgttt ttttctgcct tgtaagatca
     2821 ctacgtgtga aatgctctga gtacctttca aatataccta cttttggtgg taagtgtagg
     2881 gatgctttag gtaggtactt tgcatctgtc gaatttaaat tctaaactca cactgattaa
     2941 ggaactcagt agactacttt gcaggggcca tgttattcag tgttatctcc tccagtacaa
     3001 agaattccta gaattttgat ttgctcaggt gtgagctgac attttattgt actaccccat
     3061 tcttgtgtta agccatgtgg atttaggaca gtgatcttca aacttgcttt aacttatgct
     3121 cccttttgag aatcagcatc acttgaaatg tcaaaatatg tcaactctca tagccaaatc
     3181 aaagaagtga tcattttgac tgtgcttttt aaatctccac acacctccac ctctctccta
     3241 attctgcctg tcttaacccc tctgtcttag ttataaattt ctggtcttgt aagtctggaa
     3301 gctgataggc aatttatgaa agagataaga atgtaatgag gttccgcttt ctgagaaaca
     3361 cagaaatgat acatcctgag acataaagga aagctgctct tctgctgcct caggctgtag
     3421 cactctcaat gttgtcactc tacacataca ctttctatat acatgtacag ttgacccttg
     3481 aacagggttt gaattgcagt caacttaaat gtggattttc tttcaccttt gtcacccctg
     3541 agacagcaac accatgctct cctcttcatc ccactctgca gcctactcaa caggaagatg
     3601 atgaagatga acacctttat gatgatccac tttcacttaa tgaatagtaa acatatgttt
     3661 tcctccttat gattttagta acttttctct agcttacttt attgtaagaa tacagtatat
     3721 aagctgggtg tggtggctca tgcccataat cccagcactt tgggaggctg aggcaggcag
     3781 atcacttgag gtcaggagtt caagaccagc ctggccaaca cagtgaaacc ccatctctac
     3841 taaaaataca aaaattagtg gggcgtggtg gcggatgcct gtaatgtcag ctactctgga
     3901 ggctgaggca ggagaactgc ttgaacctgg gaggtggagt ttgcagtgag ccaagattgc
     3961 accactgcac tccagcctag gcaacagagc aagactccgt ctcaaaaaaa aaaaaaaaaa
     4021 agaatacagt atatgtatat atacacacac acaaacacac acacacacga acacatatgt
     4081 gtgtatgggt attaactgac tgtttatatt attgataggg cttccagtca acattaggct
     4141 attagtagtt aagtttttgg ggagacaaaa gttatactca gattttctac tgcaaggggg
     4201 taggcactcc taaccctcac attgttcaag ggtccactgt acatttaaac acttttctat
     4261 atgcattaga gtagccctgt catcccttca ctgaaatcat actgttctca acatggcaag
     4321 caactaacac tttttttttt ttttgagaca gggtctcact ctgttgccca ggctgcagtg
     4381 cagtggtgtg atcttggctc actgtagcct ccgcctcctg ggctcaggcg atcatgagta
     4441 cctgggacca taggtgcccg ctactacacc cagctaattt ttgtattttt agtggagata
     4501 gggttttgcc atgttgccca acctggtctt gagctcaagc gatccacctg ccccagcctc
     4561 ccaaagtgct gggattatag gtgtgagcca ccacacccag cttcaactaa cacatttacg
     4621 aacttgtata catgtatatt tagatacttt accaacttgt aaaatggtta aaggagtact
     4681 ttattatgaa aaaatataca atctttaaaa tttccttact tctacatgat ttttgtgcta
     4741 ttcccatttt tttcctcagg tgagcagctt tagtttttta ttttttttga gagagtcttc
     4801 ctctgtcacc caggctggag tgcactggtg tgttcttggc tcactgcaac ctctgcctcc
     4861 cgggttcaag tgatcttgtg cctcagcctc ccaggtagct gggattacag gcgtgtgctg
     4921 ccacgcccag ctaatttttg tatttttggt agagatgggg ttttgccatg ttggccaggc
     4981 tggtctcgaa ctcctgacct cagatgatcc acctgtcttg gcctcccaaa gagctgggaa
     5041 tataggcatg agccactgtg cttggcctca ggtgagcagc tttagtcaat gttgtgaatt
     5101 ttagatttta attagacatg caacagtttc actacctttc aggatttttg tcctgtaaca
     5161 gaggctcttg ctttttgaca gagaggtagg caggtggaga ggttatcctg ctgctgcagt
     5221 tctcaagttg ttaagtttcc tctggaaggc taacccttgt tgggaactaa cagtttcaat
     5281 accagcaagt ctaggcctgc tccaagttgg tcagctgaag aatgaacatc agaagacaca
     5341 gctgctgaaa gttgtccttt gatgagacag tgatagtgat ttggtaaaat gtcttatttt
     5401 ttaaatgtca gttatctttc tttaaaaggt tttttgaggg cagcctccag aaggagctag
     5461 agagtatatt ttatagttct attgtggttc ataccctgtt ttcgacttaa gattctggag
     5521 aatgctatga aacatctccc cagaaaaaga cagttaatta ccatatctag agcagcactg
     5581 cccaacaaaa atatagtaca ggctatacac ataattaaaa catttctagt agcttctcta
     5641 acaaaaccca ttgaaagcca attttaataa tttatataac ttagtgtatc aaaaatattt
     5701 caatatgtaa tcaacataaa attgagatac tttaccagct actagggagg ctgaggccag
     5761 agaatcactt gaacccaggg ggtggaggtt gcagtgagcc aagatcacac cattgaactc
     5821 cagcctgggc aacaagagca aaactccgtc tcaaaaaaaa agagatattt tatattcttt
     5881 ttctcatact aagtctcaaa aatctggtat attttacact taaaaacaca tgtcaaggct
     5941 aggcatggtg gctcacatct gtaatcccag cactttggga ggccaaggtg ggcagattgc
     6001 tggccaacat ggtaaaaccc catctctaaa aatataaaaa ttagctgggc gtggtggcgc
     6061 atacctgtaa tcccagctac ttgggaggct aaggcacaag aatcacttaa acaggaggca
     6121 ggggttgcag tgagctgaga tcacaccact gcactccagc ctgggtggca gagcaaaact
     6181 ttgtccccac ccctgacaaa aaacaaacaa acaaacaaaa caaaaaaaaa cctgtcaatt
     6241 cagatgctag gttttcatca gacgtactta atctgtattt agatttctta aaacttactg
     6301 tggaaaatgt atttacatac tcaagttgtt tgaaacataa ctcactgttt tccaataact
     6361 gaagtatcca cttttacatg tattaaaatt aaataaaatt agaaattcag ttctgcagtt
     6421 gcactagcca cattttaagt gtttaatagc cacacgtggt tagtggcatc tatattggac
     6481 agggcagatc tagagagaat cctgtatcta acaattttaa tttttttccc tttatgctgt
     6541 tattccttac ctagagaaac aatttccctc caaagttcct ttgaggggtc tgtttaggcc
     6601 aggccaacac aagtgaccta tgtggatttt agcatccttt ttttgaaatt tgaggtttta
     6661 tgaagcttga gtttttctgg atatttttag taatttgctg gtgtgtactt agctcaaata
     6721 cttgattgca actgtgttgg gtcaactatt tctaatggga cttttccatt tgcatgtaca
     6781 gtcactggaa actgctgggc agagaaactc taaaaggtag ttggggcaca ctttttccac
     6841 ctgtcagatt ggtgaagaat tggtgaggct gtggggaaaa tggcattctc ccacttttga
     6901 tggatatgta tccaaataaa agtcattccc atgctttctt tcatccagaa attttatttc
     6961 tagaaattta tccctccgta cttgaacaat tgtatagaga tttattcaaa atgatgttta
     7021 ctatagcact gttgctaatg gcccagtaaa aacaaccaac acatgcctat tagtttggtt
     7081 ttagttaaat gaattttgcc acatccatgt agtggaatac ctcacagctg ttatagatag
     7141 atctagatat actgaaagcc attttttctt aaattataga atgtatatat ggtatcatcc
     7201 catttgtgaa gagacatatg cttatatgtg cattaaaaaa ccgtaggata tgcaagaaac
     7261 ttaacagtgg agtgtgaaaa gtgggtgatt gggagagggg gaattccact tttcatttat
     7321 ctttctgaac cttttgaatc tttttttttt ttttttttac aatgagcatg tattcctttt
     7381 tttttttttc tttccaagat ggagtctcac tctgttgcca ggctggagtg cagtggcgca
     7441 atcttggctt actgcaacat ccacctcccg agttcaagtg atttctcctg cctcagcctc
     7501 ccaagtagct gggactacag gcatgcgcca ccacgcccag ctaatttttg tgtttttagt
     7561 agagatgggc tttcaccatg ctggccagga tggtctcgat ctcttgacct cgtgatctgc
     7621 ccacctcggc ctcccaaagt gctgggatta caggtgtgag ccactgcgcc tggcccgcat
     7681 gtattacatt tataattaaa aattcacaac tcaatgtcaa gtgtgaacct tgtgttgacc
     7741 ctgatttgag caagcaataa aaagatattt ttgagacatt tagattatga atttcacatt
     7801 tgatgatagt tactattaat tttgtgaggt gtgatattgg caatgtggtt agatgacttc
     7861 agagaaaaag gaaggtatag ttaagcaagt atggcaacat ctaataactt gggtctaggt
     7921 gatgtgtatg ggtttcatta tactgtcctc tttactgttg tatgtatttg aaaacattca
     7981 tgacaaaaaa actttttaat cagttaaata aaccatgaag aacggttaaa gaa
//
