LOCUS       NM_053025              10113 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens myosin light chain kinase (MYLK), transcript variant
            1, mRNA.
ACCESSION   NM_053025
VERSION     NM_053025.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Kim,D., Cooper,J.A. and Helfman,D.M.
  TITLE     Loss of myosin light chain kinase induces the cellular senescence
            associated secretory phenotype to promote breast epithelial cell
            migration
  JOURNAL   Sci Rep 14 (1), 25786 (2024)
   PUBMED   39468273
  REMARK    GeneRIF: Loss of myosin light chain kinase induces the cellular
            senescence associated secretory phenotype to promote breast
            epithelial cell migration.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10113)
  AUTHORS   Feng,W., Liang,H., Liu,D. and Ruan,S.
  TITLE     The SNHG12/microRNA-15b-5p/MYLK axis regulates vascular smooth
            muscle cell phenotype to affect intracranial aneurysm formation
  JOURNAL   Microvasc Res 152, 104643 (2024)
   PUBMED   38081409
  REMARK    GeneRIF: The SNHG12/microRNA-15b-5p/MYLK axis regulates vascular
            smooth muscle cell phenotype to affect intracranial aneurysm
            formation.
REFERENCE   3  (bases 1 to 10113)
  AUTHORS   Zuo,L., Kuo,W.T., Cao,F., Chanez-Paredes,S.D., Zeve,D., Mannam,P.,
            Jean-Francois,L., Day,A., Vallen Graham,W., Sweat,Y.Y.,
            Shashikanth,N., Breault,D.T. and Turner,J.R.
  TITLE     Tacrolimus-binding protein FKBP8 directs myosin light chain
            kinase-dependent barrier regulation and is a potential therapeutic
            target in Crohn's disease
  JOURNAL   Gut 72 (5), 870-881 (2023)
   PUBMED   35537812
  REMARK    GeneRIF: Tacrolimus-binding protein FKBP8 directs myosin light
            chain kinase-dependent barrier regulation and is a potential
            therapeutic target in Crohn's disease.
REFERENCE   4  (bases 1 to 10113)
  AUTHORS   Zhao,J., Ding,H., Ding,J., Shi,X., He,Y., Zhu,H., Yuan,H., Zhang,T.
            and Zhang,J.
  TITLE     The m6A methyltransferase METTL3 promotes trophoblast cell invasion
            by regulating MYLK expression
  JOURNAL   Placenta 129, 1-6 (2022)
   PUBMED   36170767
  REMARK    GeneRIF: The m(6)A methyltransferase METTL3 promotes trophoblast
            cell invasion by regulating MYLK expression.
REFERENCE   5  (bases 1 to 10113)
  AUTHORS   Ambartsumyan,L.
  TITLE     Megacystis-Microcolon-Intestinal Hypoperistalsis Syndrome Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   31070878
REFERENCE   6  (bases 1 to 10113)
  AUTHORS   Milewicz,D.M. and Cecchi,A.C.
  TITLE     Heritable Thoracic Aortic Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301299
REFERENCE   7  (bases 1 to 10113)
  AUTHORS   Ikebe,M. and Reardon,S.
  TITLE     Phosphorylation of smooth myosin light chain kinase by smooth
            muscle Ca2+/calmodulin-dependent multifunctional protein kinase
  JOURNAL   J Biol Chem 265 (16), 8975-8978 (1990)
   PUBMED   2160950
REFERENCE   8  (bases 1 to 10113)
  AUTHORS   Ikebe,M., Hartshorne,D.J. and Elzinga,M.
  TITLE     Identification, phosphorylation, and dephosphorylation of a second
            site for myosin light chain kinase on the 20,000-dalton light chain
            of smooth muscle myosin
  JOURNAL   J Biol Chem 261 (1), 36-39 (1986)
   PUBMED   3079756
REFERENCE   9  (bases 1 to 10113)
  AUTHORS   Nishikawa,M., Shirakawa,S. and Adelstein,R.S.
  TITLE     Phosphorylation of smooth muscle myosin light chain kinase by
            protein kinase C. Comparative study of the phosphorylated sites
  JOURNAL   J Biol Chem 260 (15), 8978-8983 (1985)
   PUBMED   3160694
REFERENCE   10 (bases 1 to 10113)
  AUTHORS   Nishikawa,M., de Lanerolle,P., Lincoln,T.M. and Adelstein,R.S.
  TITLE     Phosphorylation of mammalian myosin light chain kinases by the
            catalytic subunit of cyclic AMP-dependent protein kinase and by
            cyclic GMP-dependent protein kinase
  JOURNAL   J Biol Chem 259 (13), 8429-8436 (1984)
   PUBMED   6547441
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC117381.5, BC064695.1,
            U48959.2, AC020634.16, AY339601.1, AC023165.20 and BC064420.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_053025.3.
            
            Summary: This gene, a muscle member of the immunoglobulin gene
            superfamily, encodes myosin light chain kinase which is a
            calcium/calmodulin dependent enzyme. This kinase phosphorylates
            myosin regulatory light chains to facilitate myosin interaction
            with actin filaments to produce contractile activity. This gene
            encodes both smooth muscle and nonmuscle isoforms. In addition,
            using a separate promoter in an intron in the 3' region, it encodes
            telokin, a small protein identical in sequence to the C-terminus of
            myosin light chain kinase, that is independently expressed in
            smooth muscle and functions to stabilize unphosphorylated myosin
            filaments. A pseudogene is located on the p arm of chromosome 3.
            Four transcript variants that produce four isoforms of the
            calcium/calmodulin dependent enzyme have been identified as well as
            two transcripts that produce two isoforms of telokin. Additional
            variants have been identified but lack full length transcripts.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) is the full-length transcript
            and encodes the full-length nonmuscle isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: U48959.2 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000360304.8/ ENSP00000353452.3
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC117381.5         53236-53265         c
            31-159              BC064695.1         2-130
            160-160             AC117381.5         45518-45518         c
            161-477             BC064695.1         132-448
            478-750             U48959.2           285-557
            751-751             AC020634.16        94026-94026
            752-1797            U48959.2           559-1604
            1798-1798           AC020634.16        100146-100146
            1799-6057           AY339601.1         1487-5745
            6058-7848           AC023165.20        39793-41583
            7849-7866           BC064420.1         2491-2508
            7867-10113          AC023165.20        41602-43848
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q21.1"
     gene            1..10113
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /note="myosin light chain kinase"
                     /db_xref="GeneID:4638"
                     /db_xref="HGNC:HGNC:7590"
                     /db_xref="MIM:600922"
     exon            1..127
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            128..186
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            187..309
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            310..477
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     CDS             313..6057
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /EC_number="2.7.11.18"
                     /note="isoform 1 is encoded by transcript variant 1;
                     smooth muscle myosin light chain kinase; myosin, light
                     polypeptide kinase; telokin; kinase-related protein"
                     /codon_start=1
                     /product="myosin light chain kinase, smooth muscle isoform
                     1"
                     /protein_id="NP_444253.3"
                     /db_xref="CCDS:CCDS46896.1"
                     /db_xref="GeneID:4638"
                     /db_xref="HGNC:HGNC:7590"
                     /db_xref="MIM:600922"
                     /translation="MGDVKLVASSHISKTSLSVDPSRVDSMPLTEAPAFILPPRNLCI
                     KEGATAKFEGRVRGYPEPQVTWHRNGQPITSGGRFLLDCGIRGTFSLVIHAVHEEDRG
                     KYTCEATNGSGARQVTVELTVEGSFAKQLGQPVVSKTLGDRFSAPAVETRPSIWGECP
                     PKFATKLGRVVVKEGQMGRFSCKITGRPQPQVTWLKGNVPLQPSARVSVSEKNGMQVL
                     EIHGVNQDDVGVYTCLVVNGSGKASMSAELSIQGLDSANRSFVRETKATNSDVRKEVT
                     NVISKESKLDSLEAAAKSKNCSSPQRGGSPPWAANSQPQPPRESKLESCKDSPRTAPQ
                     TPVLQKTSSSITLQAARVQPEPRAPGLGVLSPSGEERKRPAPPRPATFPTRQPGLGSQ
                     DVVSKAANRRIPMEGQRDSAFPKFESKPQSQEVKENQTVKFRCEVSGIPKPEVAWFLE
                     GTPVRRQEGSIEVYEDAGSHYLCLLKARTRDSGTYSCTASNAQGQLSCSWTLQVERLA
                     VMEVAPSFSSVLKDCAVIEGQDFVLQCSVRGTPVPRITWLLNGQPIQYARSTCEAGVA
                     ELHIQDALPEDHGTYTCLAENALGQVSCSAWVTVHEKKSSRKSEYLLPVAPSKPTAPI
                     FLQGLSDLKVMDGSQVTMTVQVSGNPPPEVIWLHNGNEIQESEDFHFEQRGTQHSLCI
                     QEVFPEDTGTYTCEAWNSAGEVRTQAVLTVQEPHDGTQPWFISKPRSVTASLGQSVLI
                     SCAIAGDPFPTVHWLRDGKALCKDTGHFEVLQNEDVFTLVLKKVQPWHAGQYEILLKN
                     RVGECSCQVSLMLQNSSARALPRGREPASCEDLCGGGVGADGGGSDRYGSLRPGWPAR
                     GQGWLEEEDGEDVRGVLKRRVETRQHTEEAIRQQEVEQLDFRDLLGKKVSTKTLSEDD
                     LKEIPAEQMDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVP
                     PPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVGNAK
                     PAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVKNDVNCKRGHAGTTDNE
                     KRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWTLNGKTLKTTKFIIL
                     SQEGSLCSVSIEKALPEDRGLYKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSR
                     RPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVT
                     GTQPITCTWMKFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQ
                     AQVNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKTW
                     KELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELTTVGEKPEEPKDEVE
                     VSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLVEKKTRKVWAGKF
                     FKAYSAKEKENIRQEISIMNCLHHPKLVQCVDAFEEKANIVMVLEIVSGGELFERIID
                     EDFELTERECIKYMRQISEGVEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLAR
                     RLENAGSLKVLFGTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNE
                     TLANVTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNM
                     EAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKSSTGSPTSPLNAE
                     KLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKIEGYPDPEVVW
                     FKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKYTCKAVNSLGEATCTAELIVE
                     TMEEGEGEGEEEEE"
     exon            478..685
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            686..734
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            735..900
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1066
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1085
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1621
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..1828
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1829..1963
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2116
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2254
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2452
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2702
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..2774
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2775..3760
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            3761..3877
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            3878..3964
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            3965..4015
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            4016..4143
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            4144..4297
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            4298..4600
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            4601..4633
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            4634..4727
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            4728..4931
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            4932..5149
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            5150..5273
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            5274..5426
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            5427..5550
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            5551..5680
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            5681..5812
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            5813..10113
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7832..7837
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /note="hexamer: AATAAA"
     polyA_site      7852
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /note="major polyA site"
     regulatory      9409..9414
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /note="hexamer: AATAAT"
     polyA_site      9428
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
     regulatory      10026..10031
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /note="hexamer: ATTAAA"
     polyA_site      10051
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
     regulatory      10094..10099
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
                     /note="hexamer: AATATA"
     polyA_site      10113
                     /gene="MYLK"
                     /gene_synonym="AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;
                     MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK"
ORIGIN      
        1 gggctccccg cgccgcccgg tcggcagcag ggcgctgagc gagctcggag cccgcgctgt
       61 gcgcctgcgg ccggggcgcc ccgccgagcg ccggtgcccc ggctcccggg ccgccttcgc
      121 cgcgcgggaa ggattcttca aaattaacag aaaccaattc gggccagctg aagagaaaaa
      181 ataaaggtgg ctcccggctg cctctgctgc agttcagagc aacttcagga gcttcccagc
      241 cgagagcttc aggacgcctt tcctgtccca ctggcccagt tgccacaaca aacaacagag
      301 aagacggtga ccatggggga tgtgaagctg gttgcctcgt cacacatttc caaaacctcc
      361 ctcagtgtgg atccctcaag agttgactcc atgcccctga cagaggcccc tgctttcatt
      421 ttgccccctc ggaacctctg catcaaagaa ggagccaccg ccaagttcga agggcgggtc
      481 cggggttacc cagagcccca ggtgacatgg cacagaaacg ggcaacccat caccagcggg
      541 ggccgcttcc tgctggattg cggcatccgg gggactttca gccttgtgat tcatgctgtc
      601 catgaggagg acaggggaaa gtatacctgt gaagccacca atggcagtgg tgctcgccag
      661 gtgacagtgg agttgacagt agaaggaagt tttgcgaagc agcttggtca gcctgttgtt
      721 tccaaaacct taggggatag attttcagct ccagcagtgg agacccgtcc tagcatctgg
      781 ggggagtgcc caccaaagtt tgctaccaag ctgggccgag ttgtggtcaa agaaggacag
      841 atgggacgat tctcctgcaa gatcactggc cggccccaac cgcaggtcac ctggctcaag
      901 ggaaatgttc cactgcagcc gagtgcccgt gtgtctgtgt ctgagaagaa cggcatgcag
      961 gttctggaaa tccatggagt caaccaagat gacgtgggag tgtacacgtg cctggtggtg
     1021 aacgggtcgg ggaaggcctc gatgtcagct gaactttcca tccaaggttt ggacagtgcc
     1081 aataggtcat ttgtgagaga aacaaaagcc accaattcag atgtcaggaa agaggtgacc
     1141 aatgtaatct caaaggagtc gaagctggac agtctggagg ctgcagccaa aagcaagaac
     1201 tgctccagcc cccagagagg tggctcccca ccctgggctg caaacagcca gcctcagccc
     1261 ccaagggagt ccaagctgga gtcatgcaag gactcgccca gaacggcccc gcagaccccg
     1321 gtccttcaga agacttccag ctccatcacc ctgcaggccg caagagttca gccggaacca
     1381 agagcaccag gcctgggggt cctatcacct tctggagaag agaggaagag gccagctcct
     1441 ccccgtccag ccaccttccc caccaggcag cctggcctgg ggagccaaga tgttgtgagc
     1501 aaggctgcta acaggagaat ccccatggag ggccagaggg attcagcatt ccccaaattt
     1561 gagagcaagc cccaaagcca ggaggtcaag gaaaatcaaa ctgtcaagtt cagatgtgaa
     1621 gtttccggga ttccaaagcc tgaagtggcc tggttcctgg aaggcacccc cgtgaggaga
     1681 caggaaggca gcattgaggt ttatgaagat gctggctccc attacctctg cctgctgaaa
     1741 gcccggacca gggacagtgg gacatacagc tgcactgctt ccaacgccca aggccagctg
     1801 tcctgtagct ggaccctcca agtggaaagg cttgccgtga tggaggtggc cccctccttc
     1861 tccagtgtcc tgaaggactg cgctgttatt gagggccagg attttgtgct gcagtgctcc
     1921 gtacggggga ccccagtgcc ccggatcact tggctgctga atgggcagcc catccagtac
     1981 gctcgctcca cctgcgaggc cggcgtggct gagctccaca tccaggatgc cctgccggag
     2041 gaccatggca cctacacctg cctagctgag aatgccttgg ggcaggtgtc ctgcagcgcc
     2101 tgggtcaccg tccatgaaaa gaagagtagc aggaagagtg agtaccttct gcctgtggct
     2161 cccagcaagc ccactgcacc catcttcctg cagggcctct ctgatctcaa agtcatggat
     2221 ggaagccagg tcactatgac tgtccaagtg tcagggaatc caccccctga agtcatctgg
     2281 ctgcacaatg ggaatgagat ccaagagtca gaggacttcc actttgaaca gagaggaact
     2341 cagcacagcc tttgtatcca ggaagtgttc ccggaggaca cgggcacgta cacctgcgag
     2401 gcctggaaca gcgctggaga ggtccgcacc caggccgtgc tcacggtaca agagcctcac
     2461 gatggcaccc agccctggtt catcagtaag cctcgctcag tgacagcctc cctgggccag
     2521 agtgtcctca tctcctgcgc catagctggt gacccctttc ctaccgtgca ctggctcaga
     2581 gatggcaaag ccctctgcaa agacactggc cacttcgagg tgcttcagaa tgaggacgtg
     2641 ttcaccctgg ttctaaagaa ggtgcagccc tggcatgccg gccagtatga gatcctgctc
     2701 aagaaccggg ttggcgaatg cagttgccag gtgtcactga tgctacagaa cagctctgcc
     2761 agagcccttc cacgggggag ggagcctgcc agctgcgagg acctctgtgg tggaggagtt
     2821 ggtgctgatg gtggtggtag tgaccgctat gggtccctga ggcctggctg gccagcaaga
     2881 gggcagggtt ggctagagga ggaagacggc gaggacgtgc gaggggtgct gaagaggcgc
     2941 gtggagacga ggcagcacac tgaggaggcg atccgccagc aggaggtgga gcagctggac
     3001 ttccgagacc tcctggggaa gaaggtgagt acaaagaccc tatcggaaga cgacctgaag
     3061 gagatcccag ccgagcagat ggatttccgt gccaacctgc agcggcaagt gaagccaaag
     3121 actgtgtctg aggaagagag gaaggtgcac agcccccagc aggtcgattt tcgctctgtc
     3181 ctggccaaga aggggacttc caagaccccc gtgcctgaga aggtgccacc gccaaaacct
     3241 gccaccccgg attttcgctc agtgctgggt ggcaagaaga aattaccagc agagaatggc
     3301 agcagcagtg ccgagaccct gaatgccaag gcagtggaga gttccaagcc cctgagcaat
     3361 gcacagcctt cagggccctt gaaacccgtg ggcaacgcca agcctgctga gaccctgaag
     3421 ccaatgggca acgccaagcc tgccgagacc ctgaagccca tgggcaatgc caagcctgat
     3481 gagaacctga aatccgctag caaagaagaa ctcaagaaag acgttaagaa tgatgtgaac
     3541 tgcaagagag gccatgcagg gaccacagat aatgaaaaga gatcagagag ccaggggaca
     3601 gccccagcct tcaagcagaa gctgcaagat gttcatgtgg cagagggcaa gaagctgctg
     3661 ctccagtgcc aggtgtcttc tgacccccca gccaccatca tctggacgct gaacggaaag
     3721 accctcaaga ccaccaagtt catcatcctc tcccaggaag gctcactctg ctccgtctcc
     3781 atcgagaagg cactgcctga ggacagaggc ttatacaagt gtgtagccaa gaatgacgct
     3841 ggccaggcgg agtgctcctg ccaagtcacc gtggatgatg ctccagccag tgagaacacc
     3901 aaggccccag agatgaaatc ccggaggccc aagagctctc ttcctcccgt gctaggaact
     3961 gagagtgatg cgactgtgaa aaagaaacct gcccccaaga cacctccgaa ggcagcaatg
     4021 ccccctcaga tcatccagtt ccctgaggac cagaaggtac gcgcaggaga gtcagtggag
     4081 ctgtttggca aagtgacagg cactcagccc atcacctgta cctggatgaa gttccgaaag
     4141 cagatccagg aaagcgagca catgaaggtg gagaacagcg agaatggcag caagctcacc
     4201 atcctggccg cgcgccagga gcactgcggc tgctacacac tgctggtgga gaacaagctg
     4261 ggcagcaggc aggcccaggt caacctcact gtcgtggata agccagaccc cccagctggc
     4321 acaccttgtg cctctgacat tcggagctcc tcactgaccc tgtcctggta tggctcctca
     4381 tatgatgggg gcagtgctgt acagtcctac agcatcgaga tctgggactc agccaacaag
     4441 acgtggaagg aactagccac atgccgcagc acctctttca acgtccagga cctgctgcct
     4501 gaccacgaat ataagttccg tgtacgtgca atcaacgtgt atggaaccag tgagccaagc
     4561 caggagtctg aactcacaac ggtaggagag aaacctgaag agccgaagga tgaagtggag
     4621 gtgtcagatg atgatgagaa ggagcccgag gttgattacc ggacagtgac aatcaatact
     4681 gaacaaaaag tatctgactt ctacgacatt gaggagagat taggatctgg gaaatttgga
     4741 caggtctttc gacttgtaga aaagaaaact cgaaaagtct gggcagggaa gttcttcaag
     4801 gcatattcag caaaagagaa agagaatatc cggcaggaga ttagcatcat gaactgcctc
     4861 caccacccta agctggtcca gtgtgtggat gcctttgaag aaaaggccaa catcgtcatg
     4921 gtcctggaga tcgtgtcagg aggggagctg tttgagcgca tcattgacga ggactttgag
     4981 ctgacggagc gtgagtgcat caagtacatg cggcagatct cggagggagt ggagtacatc
     5041 cacaagcagg gcatcgtgca cctggacctc aagccggaga acatcatgtg tgtcaacaag
     5101 acgggcacca ggatcaagct catcgacttt ggtctggcca ggaggctgga gaatgcgggg
     5161 tctctgaagg tcctctttgg caccccagaa tttgtggctc ctgaagtgat caactatgag
     5221 cccatcggct acgccacaga catgtggagc atcggggtca tctgctacat cctagtcagt
     5281 ggcctttccc ccttcatggg agacaacgat aacgaaacct tggccaacgt tacctcagcc
     5341 acctgggact tcgacgacga ggcattcgat gagatctccg acgatgccaa ggatttcatc
     5401 agcaatctgc tgaagaaaga tatgaaaaac cgcctggact gcacgcagtg ccttcagcat
     5461 ccatggctaa tgaaagatac caagaacatg gaggccaaga aactctccaa ggaccggatg
     5521 aagaagtaca tggcaagaag gaaatggcag aaaacgggca atgctgtgag agccattgga
     5581 agactgtcct ctatggcaat gatctcaggg ctcagtggca ggaaatcctc aacagggtca
     5641 ccaaccagcc cgctcaatgc agaaaaacta gaatctgaag aagatgtgtc ccaagctttc
     5701 cttgaggctg ttgctgagga aaagcctcat gtaaaaccct atttctctaa gaccattcgc
     5761 gatttagaag ttgtggaggg aagtgctgct agatttgact gcaagattga aggataccca
     5821 gaccccgagg ttgtctggtt caaagatgac cagtcaatca gggagtcccg ccacttccag
     5881 atagactacg atgaggacgg gaactgctct ttaattatta gtgatgtttg cggggatgac
     5941 gatgccaagt acacctgcaa ggctgtcaac agtcttggag aagccacctg cacagcagag
     6001 ctcattgtgg aaacgatgga ggaaggtgaa ggggaagggg aagaggaaga agagtgaaac
     6061 aaagccagag aaaagcagtt tctaagtcat attaaaagga ctatttctct aaaactcaaa
     6121 aaaaaaaaaa aaactcaaga tagtaaaagc acctagtgtg atagattatc ggttaggtca
     6181 tttgtgggtt gattcttcag aaacagcagt tgatacctag cagcgttatt gatgggcatt
     6241 aatctatgtt agttggcacc ttaagatact agtgcagcta gatttcattt agggaaatca
     6301 ccagtaactt gactgaccaa ttgattttag agagaaagta accaaaccaa atatttatct
     6361 gggcaaagtc ataaattctc cacttgaatg cgctcatgaa aaataaggcc aaaacaagag
     6421 ttctgggcca cagctcagcc cagagggttc ctggggatgg gaggcctctc tctccccacc
     6481 ccctgactct agagaactgg gttttctccc agtactccag caattcattt ctgaaagcag
     6541 ttgagccact ttattccaaa gtacactgca gatgttcaaa ctctccattt ctctttcccc
     6601 ttccacctgc cagttttgct gactctcaac ttgtcatgag tgtaagcatt aaggacatta
     6661 tgcttcttcg attctgaaga caggtccctg ctcatggatg actctggctt ccttaggaaa
     6721 atatttttct tccaaaatca gtaggaaatc taaacttatc ccctctttgc agatgtctag
     6781 cagcttcaga catttggtta agaacccatg ggaaaaaaaa aatccttgct aatgtggttt
     6841 cctttgtaaa ccaggattct tatttgtgct gttatagaat atcagctctg aacgtgtggt
     6901 aaagattttt gtgtttgaat ataggagaaa tcagtttgct gaaaagttag tcttaattat
     6961 ctattggcca cgatgaaaca gatttcaact gataaagagc tggagaactc catgtacttt
     7021 ggaatctcct ccaagatagc cagagtttaa tacatcttca ttctcaacac tctccaaaga
     7081 acttgaccta ccttatgggt tccatatttt tcttcttaaa tgtgcatcaa tcatgccttg
     7141 cccccaacct ttaaatatat tcttagacct ggtaaatgca ctcagacttg cgtctttagg
     7201 aatttttaac tttctttcac tacattggca cttaaatttt ttctttataa agctttttga
     7261 aggtcataaa caaagaccat aattgatgat agacctaata catttcctct gtgtgtgtgt
     7321 gtaacattcc aaatactttt tttttctttt ccactgtttg taaggtgcaa caatttaata
     7381 tttttaaggg actttttaag agttccttaa gaaccaattt aaaattactt cagtgcaatc
     7441 ctacacagta tcaacattag aattttgata ttagtcttat gttatcttcc attctatttt
     7501 tatctgcttt ttgctgctag tttcaaactg ccagtatttt tccttttgct tttaaaatag
     7561 ttacaatatt tttcatgata gccacagtat tgccacagtt tattataata aagggttttt
     7621 atttgattta gcgcattcaa agcttttttc tatcactttt gtgttcagaa tataaccttt
     7681 gtgtgcgtgt atgttgtgtg tgtgcatgtg tggcgtatat gtgtgttaca ggttaatgcc
     7741 ttcttggaat tgtgttaatg ttctcttggt ttattatgcc atcagaatgg taaatgagaa
     7801 cactacaact gtagtcagct cacaattttt aaataaagga taccacagtg catgctgttt
     7861 gttcaatctt tgcagacttc tctttctttc catgctacca gttgtaaagg acacagctat
     7921 atcctggaaa tgaaaaacaa acactgtggt gcctagatgt gaagaactgg cttatgtggt
     7981 tgtgttttgc tatggaacag aatgatttag gaagttcttg tttatatagg tagccgaatt
     8041 tacacattta gttcaaaatt tctcttgagc atcagcttag tactatatca atcattctag
     8101 aaggatatct tatagagcag gtgtccccaa cccccggtaa gtagcctgtt aggaaccagg
     8161 tcacacataa ggaggttagc agtgggcaag tgagcaaagc atcatctata tttacagccg
     8221 atccccattg ctcgcattac cgcctgagct ctgcctcctg tcagatcagc ggcagcatca
     8281 ggttctctca ggaacgaact ctattgtgaa ctgctcatgt gagggatcta ggtttcacgc
     8341 tccttatgag aatctaacac ctgatgatct gtcactgtct cccatcaccc ctagatggga
     8401 cagtctagtt gcaggaaaac aagctcaggg cttccactga ttctacatta tgatgagttg
     8461 tatacttatt ttatattata ataaaatata ttattacaat gtaataataa tagaaataaa
     8521 atgcacaata catgtaatgt gcttgaataa tcccaaaacc accccctctc ctggtccatg
     8581 gaaaaactgt cttccatgaa agtggtccct ggtgccaaaa aggttaggga ccactgttac
     8641 agagtatcag gtcctcaaga tgctaaaatc tatatgacat ttttaacatg tgacattatc
     8701 atcatcatca tcatcatcat cactgatgat actatttacc agggcatggt ttgaattggt
     8761 gactttggtg cagttcatta ttggcagcca aatgctttat ccataccttc atattgaaga
     8821 atttgttatc aggaaactac cagtcctgct ttacaggaag tctgttatca gatatcagat
     8881 ggcaagttcc ccatgtcttc agatgttcaa acaatattgt ggatggtcta gaaagagttt
     8941 aagacatgct gttaaatgta gggctagata attctctgat tctttgatgt agtctggaaa
     9001 gaaacaatcc attgtccagt taataaatat ttagtgtttt catttttaag acactcacaa
     9061 tccacaaatg tccctaacaa tttattattt ttaaagaaaa tgacttttta ttccttgcta
     9121 gtgaaaaatg tacaatttat atgctgcact gagaaaaata acagatatac tttcttccat
     9181 tcattttcat cccaaacata taaaaaataa tccattgatt gttccttgca ttgcatatct
     9241 tattaaaaga tatttcctac atgcaactaa taagacatgc tgactgttgt cagctctaaa
     9301 tttatgtaaa gattttttat ttttgttaaa atgtttgaaa tttgcttttt gctccacacc
     9361 tcacctgttt ttgataaatc tgtgctaata agtacatagg aggtaataaa taattgagtt
     9421 gtgagaaacc caattctcca tttttcaaga aaacacaagt agcactactc tctttatccc
     9481 ttgggactcc ttcctgtaat ttgaaagggg gggctaatgt tggtggcagt tgttgctaag
     9541 gatctctgca cagagttagg gcttcactct cggcttcctg gttagatggg gccatgtgac
     9601 tagtcctagt cagtgagctg tgaccagaag tgatgtatgt accctcggga ccagagcatt
     9661 tatgtgctgg tacaagcctc tccataatga cgggcaacac atgagatggt ggctgctgca
     9721 gccatctggg tctctagcca acccaaaatg gggacataac acaagcaaga aataaacatt
     9781 tgttcttata aatcaccaag attttggggt tctcagtggc tgcaacataa cctagactat
     9841 cctgatacag gcttatcacc atgttctgga tgtgtggtga ccaggatggt agccactact
     9901 cacaagtggc tatttaaatt taagttactt taaaaaataa aataaaaaat tcagttcctc
     9961 agttgtatga gttacatttc aaatgctcga cagtcacatg tacatcactt ttcaaattat
    10021 gtgaaattaa aactgaaaag cttgtgtgaa agtctagaga gtatggtcta tagtttcatc
    10081 aattgtattg gataatatag tctcgatact caa
//
