LOCUS       NM_002475                828 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens myosin light chain 6B (MYL6B), transcript variant 2,
            mRNA.
ACCESSION   NM_002475
VERSION     NM_002475.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 828)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 828)
  AUTHORS   Xie,X., Wang,X., Liao,W., Fei,R., Wu,N., Cong,X., Chen,Q., Wei,L.,
            Wang,Y. and Chen,H.
  TITLE     MYL6B, a myosin light chain, promotes MDM2-mediated p53 degradation
            and drives HCC development
  JOURNAL   J Exp Clin Cancer Res 37 (1), 28 (2018)
   PUBMED   29439719
  REMARK    GeneRIF: our results demonstrate that MYL6B is a putative tumor
            driver gene in HCC which could promote the degradation of p53 by
            enhancing its' MDM2-mediated ubiquitination.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 828)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 828)
  AUTHORS   Atcheson,E., Hamilton,E., Pathmanathan,S., Greer,B., Harriott,P.
            and Timson,D.J.
  TITLE     IQ-motif selectivity in human IQGAP2 and IQGAP3: binding of
            calmodulin and myosin essential light chain
  JOURNAL   Biosci Rep 31 (5), 371-379 (2011)
   PUBMED   21299499
REFERENCE   6  (bases 1 to 828)
  AUTHORS   Webb,R.C.
  TITLE     Smooth muscle contraction and relaxation
  JOURNAL   Adv Physiol Educ 27 (1-4), 201-206 (2003)
   PUBMED   14627618
  REMARK    Review article
REFERENCE   7  (bases 1 to 828)
  AUTHORS   Komiyama,M., Soldati,T., von Arx,P. and Perriard,J.C.
  TITLE     The intracompartmental sorting of myosin alkali light chain
            isoproteins reflects the sequence of developmental expression as
            determined by double epitope-tagging competition
  JOURNAL   J Cell Sci 109 (Pt 8), 2089-2099 (1996)
   PUBMED   8856505
REFERENCE   8  (bases 1 to 828)
  AUTHORS   Hailstones,D.L. and Gunning,P.W.
  TITLE     Characterization of human myosin light chains 1sa and 3nm:
            implications for isoform evolution and function
  JOURNAL   Mol Cell Biol 10 (3), 1095-1104 (1990)
   PUBMED   2304459
REFERENCE   9  (bases 1 to 828)
  AUTHORS   Zimmermann,K. and Starzinski-Powitz,A.
  TITLE     A novel isoform of myosin alkali light chain isolated from human
            muscle cells
  JOURNAL   Nucleic Acids Res 17 (24), 10496 (1989)
   PUBMED   2602161
REFERENCE   10 (bases 1 to 828)
  AUTHORS   Seidel,U., Bober,E., Winter,B., Lenz,S., Lohse,P., Goedde,H.W.,
            Grzeschik,K.H. and Arnold,H.H.
  TITLE     Alkali myosin light chains in man are encoded by a multigene family
            that includes the adult skeletal muscle, the embryonic or atrial,
            and nonsarcomeric isoforms
  JOURNAL   Gene 66 (1), 135-146 (1988)
   PUBMED   2458299
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC014400.1, AC034102.32 and
            AI349553.1.
            
            On Jun 2, 2019 this sequence version replaced NM_002475.4.
            
            Summary: Myosin is a hexameric ATPase cellular motor protein. It is
            composed of two heavy chains, two nonphosphorylatable alkali light
            chains, and two phosphorylatable regulatory light chains. This gene
            encodes a myosin alkali light chain expressed in both slow-twitch
            skeletal muscle and in nonmuscle tissue. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Dec
            2010].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.506531.1,
                                           SRR1163658.126067.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000695999.1/ ENSP00000512320.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BC014400.1         168-186
            20-45               AC034102.32        8668-8693           c
            46-265              AC034102.32        7207-7426           c
            266-293             AC034102.32        6459-6486           c
            294-437             AC034102.32        6101-6244           c
            438-611             AC034102.32        5707-5880           c
            612-689             AC034102.32        3754-3831           c
            690-727             AC034102.32        3564-3601           c
            728-822             AC034102.32        3323-3417           c
            823-828             AI349553.1         14-19               c
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     gene            1..828
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /note="myosin light chain 6B"
                     /db_xref="GeneID:140465"
                     /db_xref="HGNC:HGNC:29823"
                     /db_xref="MIM:609930"
     exon            1..45
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     exon            46..265
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..718
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /note="myosin light chain 1 slow a; myosin, light
                     polypeptide 6B, alkali, smooth muscle and non-muscle;
                     smooth muscle and non-muscle myosin alkali light chain 6B;
                     myosin alkali light chain 1 slow a; smooth muscle and
                     nonmuscle myosin light chain alkali 6B; myosin, light
                     chain 6B, alkali, smooth muscle and non-muscle"
                     /codon_start=1
                     /product="myosin light chain 6B"
                     /protein_id="NP_002466.1"
                     /db_xref="CCDS:CCDS8905.1"
                     /db_xref="GeneID:140465"
                     /db_xref="HGNC:HGNC:29823"
                     /db_xref="MIM:609930"
                     /translation="MPPKKDVPVKKPAGPSISKPAAKPAAAGAPPAKTKAEPAVPQAP
                     QKTQEPPVDLSKVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTN
                     AEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGK
                     VMGAELRHVLTTLGEKMTEEEVETVLAGHEDSNGCINYEAFLKHILSV"
     exon            266..293
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     exon            294..437
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     exon            438..611
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     exon            612..689
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     exon            690..727
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     exon            728..828
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      804..809
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /note="hexamer: AATAAA"
     polyA_site      828
                     /gene="MYL6B"
                     /gene_synonym="MLC1SA"
                     /note="major polyA site"
ORIGIN      
        1 gctatgcccc gccccctccc cagccccaga cacggacccc gcaggagatg ggtgccccca
       61 tccgcacact gtcctttggc caccggacat catgcctccc aagaaggatg ttcccgtgaa
      121 gaaaccagca gggccctcca tctccaaacc tgctgctaag ccagcagcag caggggctcc
      181 tccagccaag accaaagctg agccagctgt cccccaggcc cctcagaaaa cccaggagcc
      241 tccagtcgat ctctccaaag tggtgatcga gtttaacaag gaccagctgg aggagttcaa
      301 ggaggccttc gagctgtttg accgagtggg ggatggcaag atcctgtaca gccagtgtgg
      361 ggacgtgatg agggccctgg gccagaaccc caccaacgcc gaggtgctca aggtcctggg
      421 gaaccccaag agtgatgagc tgaagtcgcg gcgtgtggac tttgagactt tcctgcccat
      481 gctccaggca gtggccaaga accgaggcca aggcacatat gaggactact tggaggggtt
      541 tcgtgtgttt gacaaggagg ggaacggcaa agtcatggga gcagagctca gacatgttct
      601 caccaccctt ggagagaaga tgactgagga ggaggtggag accgttctgg caggacacga
      661 ggacagcaac ggctgcatca actacgaggc cttcttgaaa cacatcctaa gcgtctgagt
      721 gctgcagatc cagtggggtc cggacactgg gccccgcagg cgaaagcacg ttccagccac
      781 caggaggcca cctattgttt caaaataaag actgggttcc tctcttgg
//
