LOCUS       NM_021019                708 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens myosin light chain 6 (MYL6), transcript variant 1,
            mRNA.
ACCESSION   NM_021019 NM_079424
VERSION     NM_021019.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 708)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 708)
  AUTHORS   Zanivan,S., Maione,F., Hein,M.Y., Hernandez-Fernaud,J.R.,
            Ostasiewicz,P., Giraudo,E. and Mann,M.
  TITLE     SILAC-based proteomics of human primary endothelial cell
            morphogenesis unveils tumor angiogenic markers
  JOURNAL   Mol Cell Proteomics 12 (12), 3599-3611 (2013)
   PUBMED   23979707
REFERENCE   4  (bases 1 to 708)
  AUTHORS   Revenu,C., Ubelmann,F., Hurbain,I., El-Marjou,F., Dingli,F.,
            Loew,D., Delacour,D., Gilet,J., Brot-Laroche,E., Rivero,F.,
            Louvard,D. and Robine,S.
  TITLE     A new role for the architecture of microvillar actin bundles in
            apical retention of membrane proteins
  JOURNAL   Mol Biol Cell 23 (2), 324-336 (2012)
   PUBMED   22114352
REFERENCE   5  (bases 1 to 708)
  AUTHORS   Walker,M.P., Zhang,M., Le,T.P., Wu,P., Laine,M. and Greene,G.L.
  TITLE     RAC3 is a pro-migratory co-activator of ERalpha
  JOURNAL   Oncogene 30 (17), 1984-1994 (2011)
   PUBMED   21217774
REFERENCE   6  (bases 1 to 708)
  AUTHORS   Watanabe,M., Kohri,M., Takaishi,M., Horie,R. and Higashihara,M.
  TITLE     Molecular cloning and sequencing of myosin light chains in human
            megakaryoblastic leukemia cells
  JOURNAL   J Smooth Muscle Res 37 (1), 25-38 (2001)
   PUBMED   11436981
REFERENCE   7  (bases 1 to 708)
  AUTHORS   Komiyama,M., Soldati,T., von Arx,P. and Perriard,J.C.
  TITLE     The intracompartmental sorting of myosin alkali light chain
            isoproteins reflects the sequence of developmental expression as
            determined by double epitope-tagging competition
  JOURNAL   J Cell Sci 109 (Pt 8), 2089-2099 (1996)
   PUBMED   8856505
REFERENCE   8  (bases 1 to 708)
  AUTHORS   Bora,P.S., Bora,N.S., Wu,X., Kaplan,H.J. and Lange,L.G.
  TITLE     Molecular cloning, sequencing, and characterization of smooth
            muscle myosin alkali light chain from human eye cDNA: homology with
            myocardial fatty acid ethyl ester synthase-III cDNA
  JOURNAL   Genomics 19 (1), 186-188 (1994)
   PUBMED   8188229
REFERENCE   9  (bases 1 to 708)
  AUTHORS   Hailstones,D.L. and Gunning,P.W.
  TITLE     Characterization of human myosin light chains 1sa and 3nm:
            implications for isoform evolution and function
  JOURNAL   Mol Cell Biol 10 (3), 1095-1104 (1990)
   PUBMED   2304459
REFERENCE   10 (bases 1 to 708)
  AUTHORS   Lenz,S., Lohse,P., Seidel,U. and Arnold,H.H.
  TITLE     The alkali light chains of human smooth and nonmuscle myosins are
            encoded by a single gene. Tissue-specific expression by alternative
            splicing pathways
  JOURNAL   J Biol Chem 264 (15), 9009-9015 (1989)
   PUBMED   2722814
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG283793.1, BC017455.1 and
            CA427580.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_021019.4.
            
            Summary: Myosin is a hexameric ATPase cellular motor protein. It is
            composed of two heavy chains, two nonphosphorylatable alkali light
            chains, and two phosphorylatable regulatory light chains. This gene
            encodes a myosin alkali light chain that is expressed in smooth
            muscle and non-muscle tissues. Genomic sequences representing
            several pseudogenes have been described and two transcript variants
            encoding different isoforms have been identified for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1), which is the major non-muscle
            transcript, represents the longer transcript and encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.506614.1,
                                           SRR5189667.249246.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000550697.6/ ENSP00000446955.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BG283793.1         99-113
            16-702              BC017455.1         1-687
            703-708             CA427580.1         22-27               c
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.2"
     gene            1..708
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /note="myosin light chain 6"
                     /db_xref="GeneID:4637"
                     /db_xref="HGNC:HGNC:7587"
                     /db_xref="MIM:609931"
     exon            1..46
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..499
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myosin, light polypeptide 6, alkali, smooth muscle and
                     non-muscle; myosin light chain alkali 3; myosin light
                     chain A3; 17 kDa myosin light chain; myosin, light chain
                     6, alkali, smooth muscle and non-muscle"
                     /codon_start=1
                     /product="myosin light polypeptide 6 isoform 1"
                     /protein_id="NP_066299.2"
                     /db_xref="CCDS:CCDS8906.1"
                     /db_xref="GeneID:4637"
                     /db_xref="HGNC:HGNC:7587"
                     /db_xref="MIM:609931"
                     /translation="MCDFTEDQTAEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPT
                     NAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNG
                     TVMGAEIRHVLVTLGEKMTEEEVEMLVAGHEDSNGCINYEAFVRHILSG"
     exon            47..74
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /inference="alignment:Splign:2.1.0"
     exon            75..218
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /inference="alignment:Splign:2.1.0"
     exon            219..392
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /inference="alignment:Splign:2.1.0"
     exon            393..470
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /inference="alignment:Splign:2.1.0"
     exon            471..515
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /inference="alignment:Splign:2.1.0"
     exon            516..708
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      685..690
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /note="hexamer: AATAAA"
     polyA_site      708
                     /gene="MYL6"
                     /gene_synonym="ESMLC; LC17; LC17-GI; LC17-NM; LC17A;
                     LC17B; MLC-3; MLC1SM; MLC3NM; MLC3SM"
                     /note="major polyA site"
ORIGIN      
        1 gccattactg caggaaaagg tcccggagag ctgagcagtc aagatgtgtg acttcaccga
       61 agaccagacc gcagagttca aggaggcctt ccagctgttt gaccgaacag gtgatggcaa
      121 gatcctgtac agccagtgtg gggatgtgat gagggccctg ggccagaacc ctaccaacgc
      181 cgaggtgctc aaggtcctgg ggaaccccaa gagtgatgag atgaatgtga aggtgctgga
      241 ctttgagcac tttctgccca tgctgcagac agtggccaag aacaaggacc agggcaccta
      301 tgaggattat gtcgaaggac ttcgggtgtt tgacaaggaa ggaaatggca ccgtcatggg
      361 tgctgaaatc cggcatgttc ttgtcacact gggtgagaag atgacagagg aagaagtaga
      421 gatgctggtg gcagggcatg aggacagcaa tggttgtatc aactatgaag cgtttgtgag
      481 gcatatcctg tcggggtgac gggcccatgg ggcggagctc gtccgcatgg tgctgaatgg
      541 ctgaggacct tcccagtctc cccagagtcc gtgcctttcc ctgtgtgaat tttgtatcta
      601 gcctaaagtt tccctaggct ttcttgtctc agcaactttc ccatcttgtc tctcttggat
      661 gatgtttgcc gtcagcattc accaaataaa cttgctctct gggccctc
//
