LOCUS       NM_033546               1163 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens myosin light chain 12B (MYL12B), transcript variant 2,
            mRNA.
ACCESSION   NM_033546
VERSION     NM_033546.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1163)
  AUTHORS   Cobb,J., Cule,E., Moncrieffe,H., Hinks,A., Ursu,S., Patrick,F.,
            Kassoumeri,L., Flynn,E., Bulatovic,M., Wulffraat,N., van Zelst,B.,
            de Jonge,R., Bohm,M., Dolezalova,P., Hirani,S., Newman,S.,
            Whitworth,P., Southwood,T.R., De Iorio,M., Wedderburn,L.R. and
            Thomson,W.
  CONSRTM   Childhood Arthritis Response to Medication Study (CHARMS);
            Childhood Arthritis Prospective Study (CAPS); BSPAR study group
  TITLE     Genome-wide data reveal novel genes for methotrexate response in a
            large cohort of juvenile idiopathic arthritis cases
  JOURNAL   Pharmacogenomics J 14 (4), 356-364 (2014)
   PUBMED   24709693
REFERENCE   4  (bases 1 to 1163)
  AUTHORS   Kiyomitsu,T. and Cheeseman,I.M.
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately
            position the spindle in anaphase
  JOURNAL   Cell 154 (2), 391-402 (2013)
   PUBMED   23870127
  REMARK    Erratum:[Cell. 2013 Sep 12;154(6):1401]
REFERENCE   5  (bases 1 to 1163)
  AUTHORS   Lyons,P.A., Rayner,T.F., Trivedi,S., Holle,J.U., Watts,R.A.,
            Jayne,D.R., Baslund,B., Brenchley,P., Bruchfeld,A., Chaudhry,A.N.,
            Cohen Tervaert,J.W., Deloukas,P., Feighery,C., Gross,W.L.,
            Guillevin,L., Gunnarsson,I., Harper,L., Hruskova,Z., Little,M.A.,
            Martorana,D., Neumann,T., Ohlsson,S., Padmanabhan,S., Pusey,C.D.,
            Salama,A.D., Sanders,J.S., Savage,C.O., Segelmark,M.,
            Stegeman,C.A., Tesar,V., Vaglio,A., Wieczorek,S., Wilde,B.,
            Zwerina,J., Rees,A.J., Clayton,D.G. and Smith,K.G.
  TITLE     Genetically distinct subsets within ANCA-associated vasculitis
  JOURNAL   N Engl J Med 367 (3), 214-223 (2012)
   PUBMED   22808956
REFERENCE   6  (bases 1 to 1163)
  AUTHORS   Umeda,D., Tachibana,H. and Yamada,K.
  TITLE     Epigallocatechin-3-O-gallate disrupts stress fibers and the
            contractile ring by reducing myosin regulatory light chain
            phosphorylation mediated through the target molecule 67 kDa laminin
            receptor
  JOURNAL   Biochem Biophys Res Commun 333 (2), 628-635 (2005)
   PUBMED   15946647
  REMARK    GeneRIF: These results suggest that EGCG inhibits the cell growth
            by reducing the MRLC phosphorylation and this effect is mediated by
            the 67LR.
REFERENCE   7  (bases 1 to 1163)
  AUTHORS   Li,T.B., Liu,X.H., Feng,S., Hu,Y., Yang,W.X., Han,Y., Wang,Y.G. and
            Gong,L.M.
  TITLE     Characterization of MR-1, a novel myofibrillogenesis regulator in
            human muscle
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 36 (6), 412-418 (2004)
   PUBMED   15188056
REFERENCE   8  (bases 1 to 1163)
  AUTHORS   Webb,R.C.
  TITLE     Smooth muscle contraction and relaxation
  JOURNAL   Adv Physiol Educ 27 (1-4), 201-206 (2003)
   PUBMED   14627618
  REMARK    Review article
REFERENCE   9  (bases 1 to 1163)
  AUTHORS   Iwasaki,T., Murata-Hori,M., Ishitobi,S. and Hosoya,H.
  TITLE     Diphosphorylated MRLC is required for organization of stress fibers
            in interphase cells and the contractile ring in dividing cells
  JOURNAL   Cell Struct Funct 26 (6), 677-683 (2001)
   PUBMED   11942626
REFERENCE   10 (bases 1 to 1163)
  AUTHORS   Olsson,P.A., Korhonen,L., Mercer,E.A. and Lindholm,D.
  TITLE     MIR is a novel ERM-like protein that interacts with myosin
            regulatory light chain and inhibits neurite outgrowth
  JOURNAL   J Biol Chem 274 (51), 36288-36292 (1999)
   PUBMED   10593918
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AP005329.3 and CA307094.1.
            
            On Feb 27, 2020 this sequence version replaced NM_033546.3.
            
            Summary: The activity of nonmuscle myosin II (see MYH9; MIM 160775)
            is regulated by phosphorylation of a regulatory light chain, such
            as MRLC2. This phosphorylation results in higher MgATPase activity
            and the assembly of myosin II filaments (Iwasaki et al., 2001
            [PubMed 11942626]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1-3 all encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK291726.1, BM768392.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000237500.10/ ENSP00000237500.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               AP005329.3         142572-142676
            106-304             AP005329.3         153323-153521
            305-466             AP005329.3         157692-157853
            467-930             AP005329.3         158204-158667
            931-986             CA307094.1         17-72               c
            987-1163            AP005329.3         158724-158900
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.31"
     gene            1..1163
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="myosin light chain 12B"
                     /db_xref="GeneID:103910"
                     /db_xref="HGNC:HGNC:29827"
                     /db_xref="MIM:609211"
     exon            1..105
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     exon            106..304
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..639
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="myosin regulatory light chain 2; myosin regulatory
                     light chain MRLC2; MLC-2; MLC20; MLC-2A; SHUJUN-1; myosin
                     regulatory light chain 20 kDa; myosin, light chain 12B,
                     regulatory"
                     /codon_start=1
                     /product="myosin regulatory light chain 12B"
                     /protein_id="NP_291024.1"
                     /db_xref="CCDS:CCDS11831.1"
                     /db_xref="GeneID:103910"
                     /db_xref="HGNC:HGNC:29827"
                     /db_xref="MIM:609211"
                     /translation="MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQN
                     RDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDV
                     IRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFT
                     RILKHGAKDKDD"
     exon            305..466
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     exon            467..1163
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      857..862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AATAAA"
     regulatory      867..872
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AACAAA"
     polyA_site      884
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="major polyA site"
     polyA_site      890
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
     regulatory      935..940
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AATAAA"
     regulatory      954..959
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AATAAA"
     polyA_site      959
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
     polyA_site      981
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
     polyA_site      1002
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
     regulatory      1138..1143
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AGTAAA"
     polyA_site      1163
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
ORIGIN      
        1 aggcaggaag tgtcggcgcc gccactgtcc ggccacagcc taacgctctt cgctgtcgtt
       61 tgtggtctcg cgcagggcgg ccccggttct ggtgtttggc gtcggaatta aacaaccacc
      121 atgtcgagca aaaaggcaaa gaccaagacc accaagaagc gccctcagcg tgcaacatcc
      181 aatgtgtttg ccatgtttga ccagtcacag attcaggagt tcaaagaggc cttcaacatg
      241 attgatcaga acagagatgg cttcatcgac aaggaagatt tgcatgatat gcttgcttct
      301 ctagggaaga atcccactga tgcatacctt gatgccatga tgaatgaggc cccagggccc
      361 atcaatttca ccatgttcct gaccatgttt ggtgagaagt taaatggcac agatcctgaa
      421 gatgtcatca gaaacgcctt tgcttgcttt gatgaagaag caacaggcac cattcaggaa
      481 gattacctaa gagagctgct gacaaccatg ggggatcggt ttacagatga ggaagtggat
      541 gagctgtaca gagaagcacc tattgacaaa aaggggaatt tcaattacat cgagttcaca
      601 cgcatcctga aacatggagc caaagacaaa gatgactgaa agaactttag ctaaaatctt
      661 ccagttacat tgtcttactc tcttttactt ctcagacact tcccccaccc tcatagaacc
      721 tgttgcatgc aacttagttt cacagctttg cctcttcttt ttgatgtatt tattccagac
      781 ctttctgcca cttagcactt gtataatcag actggaaatg gggatgaggg tgtaaattgt
      841 attgaaaaag atcgcgaata aaaatcaaca aatgtgaaag cccagaaaaa tatattcgta
      901 tttctggttt tgctggattt ttacattttt atataataaa aatgttattt tgaaataaag
      961 attatgctga ctcaaatgca atgtaatggc aagttaaaca aatgattgat aggtgggggt
     1021 agtccttttt tcaggttttc aacttcatat gattcaggtt ttatggtaaa attgattttt
     1081 cacatatatg ggacagtgtt cttcagaact tttaagtacc ctttaacatt attatgcagt
     1141 aaaaactagt tatgccattg ttt
//
