LOCUS       NM_001144944            1041 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens myosin light chain 12B (MYL12B), transcript variant 1,
            mRNA.
ACCESSION   NM_001144944
VERSION     NM_001144944.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1041)
  AUTHORS   Cobb,J., Cule,E., Moncrieffe,H., Hinks,A., Ursu,S., Patrick,F.,
            Kassoumeri,L., Flynn,E., Bulatovic,M., Wulffraat,N., van Zelst,B.,
            de Jonge,R., Bohm,M., Dolezalova,P., Hirani,S., Newman,S.,
            Whitworth,P., Southwood,T.R., De Iorio,M., Wedderburn,L.R. and
            Thomson,W.
  CONSRTM   Childhood Arthritis Response to Medication Study (CHARMS);
            Childhood Arthritis Prospective Study (CAPS); BSPAR study group
  TITLE     Genome-wide data reveal novel genes for methotrexate response in a
            large cohort of juvenile idiopathic arthritis cases
  JOURNAL   Pharmacogenomics J 14 (4), 356-364 (2014)
   PUBMED   24709693
REFERENCE   4  (bases 1 to 1041)
  AUTHORS   Kiyomitsu,T. and Cheeseman,I.M.
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately
            position the spindle in anaphase
  JOURNAL   Cell 154 (2), 391-402 (2013)
   PUBMED   23870127
  REMARK    Erratum:[Cell. 2013 Sep 12;154(6):1401]
REFERENCE   5  (bases 1 to 1041)
  AUTHORS   Lyons,P.A., Rayner,T.F., Trivedi,S., Holle,J.U., Watts,R.A.,
            Jayne,D.R., Baslund,B., Brenchley,P., Bruchfeld,A., Chaudhry,A.N.,
            Cohen Tervaert,J.W., Deloukas,P., Feighery,C., Gross,W.L.,
            Guillevin,L., Gunnarsson,I., Harper,L., Hruskova,Z., Little,M.A.,
            Martorana,D., Neumann,T., Ohlsson,S., Padmanabhan,S., Pusey,C.D.,
            Salama,A.D., Sanders,J.S., Savage,C.O., Segelmark,M.,
            Stegeman,C.A., Tesar,V., Vaglio,A., Wieczorek,S., Wilde,B.,
            Zwerina,J., Rees,A.J., Clayton,D.G. and Smith,K.G.
  TITLE     Genetically distinct subsets within ANCA-associated vasculitis
  JOURNAL   N Engl J Med 367 (3), 214-223 (2012)
   PUBMED   22808956
REFERENCE   6  (bases 1 to 1041)
  AUTHORS   Umeda,D., Tachibana,H. and Yamada,K.
  TITLE     Epigallocatechin-3-O-gallate disrupts stress fibers and the
            contractile ring by reducing myosin regulatory light chain
            phosphorylation mediated through the target molecule 67 kDa laminin
            receptor
  JOURNAL   Biochem Biophys Res Commun 333 (2), 628-635 (2005)
   PUBMED   15946647
  REMARK    GeneRIF: These results suggest that EGCG inhibits the cell growth
            by reducing the MRLC phosphorylation and this effect is mediated by
            the 67LR.
REFERENCE   7  (bases 1 to 1041)
  AUTHORS   Li,T.B., Liu,X.H., Feng,S., Hu,Y., Yang,W.X., Han,Y., Wang,Y.G. and
            Gong,L.M.
  TITLE     Characterization of MR-1, a novel myofibrillogenesis regulator in
            human muscle
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 36 (6), 412-418 (2004)
   PUBMED   15188056
REFERENCE   8  (bases 1 to 1041)
  AUTHORS   Webb,R.C.
  TITLE     Smooth muscle contraction and relaxation
  JOURNAL   Adv Physiol Educ 27 (1-4), 201-206 (2003)
   PUBMED   14627618
  REMARK    Review article
REFERENCE   9  (bases 1 to 1041)
  AUTHORS   Iwasaki,T., Murata-Hori,M., Ishitobi,S. and Hosoya,H.
  TITLE     Diphosphorylated MRLC is required for organization of stress fibers
            in interphase cells and the contractile ring in dividing cells
  JOURNAL   Cell Struct Funct 26 (6), 677-683 (2001)
   PUBMED   11942626
REFERENCE   10 (bases 1 to 1041)
  AUTHORS   Olsson,P.A., Korhonen,L., Mercer,E.A. and Lindholm,D.
  TITLE     MIR is a novel ERM-like protein that interacts with myosin
            regulatory light chain and inhibits neurite outgrowth
  JOURNAL   J Biol Chem 274 (51), 36288-36292 (1999)
   PUBMED   10593918
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BG696903.1 and CA307094.1.
            
            Summary: The activity of nonmuscle myosin II (see MYH9; MIM 160775)
            is regulated by phosphorylation of a regulatory light chain, such
            as MRLC2. This phosphorylation results in higher MgATPase activity
            and the assembly of myosin II filaments (Iwasaki et al., 2001
            [PubMed 11942626]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-3 all encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BG696903.1, SRR7346977.517958.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-746               BG696903.1         2-747
            747-1041            CA307094.1         1-295               c
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.31"
     gene            1..1041
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="myosin light chain 12B"
                     /db_xref="GeneID:103910"
                     /db_xref="HGNC:HGNC:29827"
                     /db_xref="MIM:609211"
     exon            1..144
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     exon            145..343
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..678
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="myosin regulatory light chain 2; myosin regulatory
                     light chain MRLC2; MLC-2; MLC20; MLC-2A; SHUJUN-1; myosin
                     regulatory light chain 20 kDa; myosin, light chain 12B,
                     regulatory"
                     /codon_start=1
                     /product="myosin regulatory light chain 12B"
                     /protein_id="NP_001138416.1"
                     /db_xref="CCDS:CCDS11831.1"
                     /db_xref="GeneID:103910"
                     /db_xref="HGNC:HGNC:29827"
                     /db_xref="MIM:609211"
                     /translation="MSSKKAKTKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQN
                     RDGFIDKEDLHDMLASLGKNPTDAYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDV
                     IRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFT
                     RILKHGAKDKDD"
     exon            344..505
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     exon            506..1025
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      896..901
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AATAAA"
     regulatory      906..911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AACAAA"
     polyA_site      923
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="major polyA site"
     polyA_site      929
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
     regulatory      974..979
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AATAAA"
     regulatory      993..998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
                     /note="hexamer: AATAAA"
     polyA_site      998
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
     polyA_site      1020
                     /gene="MYL12B"
                     /gene_synonym="MLC-B; MRLC2"
ORIGIN      
        1 ctgccctccc ggcgccgcgg cgggtcctgg cggtggattg gggccgaccc gggcgtgttc
       61 gtgtggagca cctgccctgt gcccaacact atcctagatg ctttggaggg tggaggagcc
      121 tcctgttgca cggtcttgcc taagaattaa acaaccacca tgtcgagcaa aaaggcaaag
      181 accaagacca ccaagaagcg ccctcagcgt gcaacatcca atgtgtttgc catgtttgac
      241 cagtcacaga ttcaggagtt caaagaggcc ttcaacatga ttgatcagaa cagagatggc
      301 ttcatcgaca aggaagattt gcatgatatg cttgcttctc tagggaagaa tcccactgat
      361 gcataccttg atgccatgat gaatgaggcc ccagggccca tcaatttcac catgttcctg
      421 accatgtttg gtgagaagtt aaatggcaca gatcctgaag atgtcatcag aaacgccttt
      481 gcttgctttg atgaagaagc aacaggcacc attcaggaag attacctaag agagctgctg
      541 acaaccatgg gggatcggtt tacagatgag gaagtggatg agctgtacag agaagcacct
      601 attgacaaaa aggggaattt caattacatc gagttcacac gcatcctgaa acatggagcc
      661 aaagacaaag atgactgaaa gaactttagc taaaatcttc cagttacatt gtcttactct
      721 cttttacttc tcagacactt cccccaccct catagaacct gttgcatgca acttagtttc
      781 acagctttgc ctcttctttt tgatgtattt attccagacc tttctgccac ttagcacttg
      841 tataatcaga ctggaaatgg ggatgagggt gtaaattgta ttgaaaaaga tcgcgaataa
      901 aaatcaacaa atgtgaaagc ccagaaaaat atattcgtat ttctggtttt gctggatttt
      961 tacattttta tataataaaa atgttatttt gaaataaaga ttatgctgac tcaaatgcaa
     1021 tgtaaaaaaa aaaaaaaaaa a
//
