LOCUS       NP_006462                171 aa            linear   PRI 30-APR-2025
DEFINITION  myosin regulatory light chain 12A isoform 1 [Homo sapiens].
ACCESSION   NP_006462
VERSION     NP_006462.1
DBSOURCE    REFSEQ: accession NM_006471.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 171)
  AUTHORS   Frobel,J., Cadeddu,R.P., Hartwig,S., Bruns,I., Wilk,C.M.,
            Kundgen,A., Fischer,J.C., Schroeder,T., Steidl,U.G., Germing,U.,
            Lehr,S., Haas,R. and Czibere,A.
  TITLE     Platelet proteome analysis reveals integrin-dependent aggregation
            defects in patients with myelodysplastic syndromes
  JOURNAL   Mol Cell Proteomics 12 (5), 1272-1280 (2013)
   PUBMED   23382103
REFERENCE   2  (residues 1 to 171)
  AUTHORS   Hopker,K., Hagmann,H., Khurshid,S., Chen,S., Hasskamp,P.,
            Seeger-Nukpezah,T., Schilberg,K., Heukamp,L., Lamkemeyer,T.,
            Sos,M.L., Thomas,R.K., Lowery,D., Roels,F., Fischer,M.,
            Liebau,M.C., Resch,U., Kisner,T., Rother,F., Bartram,M.P.,
            Muller,R.U., Fabretti,F., Kurschat,P., Schumacher,B., Gaestel,M.,
            Medema,R.H., Yaffe,M.B., Schermer,B., Reinhardt,H.C. and Benzing,T.
  TITLE     AATF/Che-1 acts as a phosphorylation-dependent molecular modulator
            to repress p53-driven apoptosis
  JOURNAL   EMBO J 31 (20), 3961-3975 (2012)
   PUBMED   22909821
REFERENCE   3  (residues 1 to 171)
  AUTHORS   Tan,I., Lai,J., Yong,J., Li,S.F. and Leung,T.
  TITLE     Chelerythrine perturbs lamellar actomyosin filaments by selective
            inhibition of myotonic dystrophy kinase-related Cdc42-binding
            kinase
  JOURNAL   FEBS Lett 585 (9), 1260-1268 (2011)
   PUBMED   21457715
REFERENCE   4  (residues 1 to 171)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   5  (residues 1 to 171)
  AUTHORS   Bouwmeester,T., Bauch,A., Ruffner,H., Angrand,P.O., Bergamini,G.,
            Croughton,K., Cruciat,C., Eberhard,D., Gagneur,J., Ghidelli,S.,
            Hopf,C., Huhse,B., Mangano,R., Michon,A.M., Schirle,M., Schlegl,J.,
            Schwab,M., Stein,M.A., Bauer,A., Casari,G., Drewes,G., Gavin,A.C.,
            Jackson,D.B., Joberty,G., Neubauer,G., Rick,J., Kuster,B. and
            Superti-Furga,G.
  TITLE     A physical and functional map of the human TNF-alpha/NF-kappa B
            signal transduction pathway
  JOURNAL   Nat Cell Biol 6 (2), 97-105 (2004)
   PUBMED   14743216
  REMARK    Erratum:[Nat Cell Biol. 2004 May;6(5):465]
REFERENCE   6  (residues 1 to 171)
  AUTHORS   Iwasaki,T., Murata-Hori,M., Ishitobi,S. and Hosoya,H.
  TITLE     Diphosphorylated MRLC is required for organization of stress fibers
            in interphase cells and the contractile ring in dividing cells
  JOURNAL   Cell Struct Funct 26 (6), 677-683 (2001)
   PUBMED   11942626
REFERENCE   7  (residues 1 to 171)
  AUTHORS   Grant,J.W., Zhong,R.Q., McEwen,P.M. and Church,S.L.
  TITLE     Human nonsarcomeric 20,000 Da myosin regulatory light chain cDNA
  JOURNAL   Nucleic Acids Res 18 (19), 5892 (1990)
   PUBMED   2216787
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC031972.1 and BC032748.1.
            
            Summary: This gene encodes a nonsarcomeric myosin regulatory light
            chain. This protein is activated by phosphorylation and regulates
            smooth muscle and non-muscle cell contraction. This protein may
            also be involved in DNA damage repair by sequestering the
            transcriptional regulator apoptosis-antagonizing transcription
            factor (AATF)/Che-1 which functions as a repressor of p53-driven
            apoptosis. Alternate splicing results in multiple transcript
            variants. A pseudogene of this gene is found on chromosome
            8.[provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (1) encodes the shorter isoform
            (1). Variants 1, 2 and 3 encode the same isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU794621.1, BC031972.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000217652.8/ ENSP00000217652.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.31"
     Protein         1..171
                     /product="myosin regulatory light chain 12A isoform 1"
                     /note="myosin regulatory light chain 3; epididymis
                     secretory protein Li 24; myosin regulatory light chain
                     MRLC3; myosin regulatory light chain 12A; myosin, light
                     polypeptide, regulatory, non-sarcomeric (20kD); myosin
                     RLC; myosin regulatory light chain 2, nonsarcomeric;
                     myosin, light chain 12A, regulatory, non-sarcomeric"
                     /calculated_mol_wt=19663
     Site            18
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by MLCK.
                     /evidence=ECO:0000269|PubMed:11942626; propagated from
                     UniProtKB/Swiss-Prot (P19105.2)"
     Site            19
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by MLCK.
                     /evidence=ECO:0000269|PubMed:11942626; propagated from
                     UniProtKB/Swiss-Prot (P19105.2)"
     Region          24..162
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..171
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /coded_by="NM_006471.4:125..640"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS11830.1"
                     /db_xref="GeneID:10627"
                     /db_xref="HGNC:HGNC:16701"
ORIGIN      
        1 msskrtktkt kkrpqratsn vfamfdqsqi qefkeafnmi dqnrdgfidk edlhdmlasl
       61 gknptdeyld ammneapgpi nftmfltmfg eklngtdped virnafacfd eeatgtiqed
      121 ylrellttmg drftdeevde lyreapidkk gnfnyieftr ilkhgakdkd d
//
