LOCUS       NM_001303049             900 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens myosin light chain 12A (MYL12A), transcript variant 4,
            mRNA.
ACCESSION   NM_001303049 XM_005258077
VERSION     NM_001303049.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Frobel,J., Cadeddu,R.P., Hartwig,S., Bruns,I., Wilk,C.M.,
            Kundgen,A., Fischer,J.C., Schroeder,T., Steidl,U.G., Germing,U.,
            Lehr,S., Haas,R. and Czibere,A.
  TITLE     Platelet proteome analysis reveals integrin-dependent aggregation
            defects in patients with myelodysplastic syndromes
  JOURNAL   Mol Cell Proteomics 12 (5), 1272-1280 (2013)
   PUBMED   23382103
REFERENCE   2  (bases 1 to 900)
  AUTHORS   Hopker,K., Hagmann,H., Khurshid,S., Chen,S., Hasskamp,P.,
            Seeger-Nukpezah,T., Schilberg,K., Heukamp,L., Lamkemeyer,T.,
            Sos,M.L., Thomas,R.K., Lowery,D., Roels,F., Fischer,M.,
            Liebau,M.C., Resch,U., Kisner,T., Rother,F., Bartram,M.P.,
            Muller,R.U., Fabretti,F., Kurschat,P., Schumacher,B., Gaestel,M.,
            Medema,R.H., Yaffe,M.B., Schermer,B., Reinhardt,H.C. and Benzing,T.
  TITLE     AATF/Che-1 acts as a phosphorylation-dependent molecular modulator
            to repress p53-driven apoptosis
  JOURNAL   EMBO J 31 (20), 3961-3975 (2012)
   PUBMED   22909821
REFERENCE   3  (bases 1 to 900)
  AUTHORS   Tan,I., Lai,J., Yong,J., Li,S.F. and Leung,T.
  TITLE     Chelerythrine perturbs lamellar actomyosin filaments by selective
            inhibition of myotonic dystrophy kinase-related Cdc42-binding
            kinase
  JOURNAL   FEBS Lett 585 (9), 1260-1268 (2011)
   PUBMED   21457715
REFERENCE   4  (bases 1 to 900)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   5  (bases 1 to 900)
  AUTHORS   Bouwmeester,T., Bauch,A., Ruffner,H., Angrand,P.O., Bergamini,G.,
            Croughton,K., Cruciat,C., Eberhard,D., Gagneur,J., Ghidelli,S.,
            Hopf,C., Huhse,B., Mangano,R., Michon,A.M., Schirle,M., Schlegl,J.,
            Schwab,M., Stein,M.A., Bauer,A., Casari,G., Drewes,G., Gavin,A.C.,
            Jackson,D.B., Joberty,G., Neubauer,G., Rick,J., Kuster,B. and
            Superti-Furga,G.
  TITLE     A physical and functional map of the human TNF-alpha/NF-kappa B
            signal transduction pathway
  JOURNAL   Nat Cell Biol 6 (2), 97-105 (2004)
   PUBMED   14743216
  REMARK    Erratum:[Nat Cell Biol. 2004 May;6(5):465]
REFERENCE   6  (bases 1 to 900)
  AUTHORS   Iwasaki,T., Murata-Hori,M., Ishitobi,S. and Hosoya,H.
  TITLE     Diphosphorylated MRLC is required for organization of stress fibers
            in interphase cells and the contractile ring in dividing cells
  JOURNAL   Cell Struct Funct 26 (6), 677-683 (2001)
   PUBMED   11942626
REFERENCE   7  (bases 1 to 900)
  AUTHORS   Grant,J.W., Zhong,R.Q., McEwen,P.M. and Church,S.L.
  TITLE     Human nonsarcomeric 20,000 Da myosin regulatory light chain cDNA
  JOURNAL   Nucleic Acids Res 18 (19), 5892 (1990)
   PUBMED   2216787
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC016372.1 and AB046614.1.
            
            On Aug 13, 2020 this sequence version replaced NM_001303049.1.
            
            Summary: This gene encodes a nonsarcomeric myosin regulatory light
            chain. This protein is activated by phosphorylation and regulates
            smooth muscle and non-muscle cell contraction. This protein may
            also be involved in DNA damage repair by sequestering the
            transcriptional regulator apoptosis-antagonizing transcription
            factor (AATF)/Che-1 which functions as a repressor of p53-driven
            apoptosis. Alternate splicing results in multiple transcript
            variants. A pseudogene of this gene is found on chromosome
            8.[provided by RefSeq, Dec 2014].
            
            Transcript Variant: This variant (4) uses an alternate exon in the
            5' UTR and 5' coding region compared to variant 1. These
            differences result in the use of an alternate start codon compared
            to variant 1. The encoded isoform (2) has a longer N-terminus
            compare to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016372.1, BF790442.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-777               BC016372.1         5-781
            778-900             AB046614.1         831-953
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18p11.31"
     gene            1..900
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /note="myosin light chain 12A"
                     /db_xref="GeneID:10627"
                     /db_xref="HGNC:HGNC:16701"
     exon            1..51
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..582
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /note="isoform 2 is encoded by transcript variant 4;
                     myosin regulatory light chain 3; epididymis secretory
                     protein Li 24; myosin regulatory light chain MRLC3; myosin
                     regulatory light chain 12A; myosin, light polypeptide,
                     regulatory, non-sarcomeric (20kD); myosin RLC; myosin
                     regulatory light chain 2, nonsarcomeric; myosin, light
                     chain 12A, regulatory, non-sarcomeric"
                     /codon_start=1
                     /product="myosin regulatory light chain 12A isoform 2"
                     /protein_id="NP_001289978.1"
                     /db_xref="CCDS:CCDS77145.1"
                     /db_xref="GeneID:10627"
                     /db_xref="HGNC:HGNC:16701"
                     /translation="MDLTTTMSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFN
                     MIDQNRDGFIDKEDLHDMLASLGKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGT
                     DPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFN
                     YIEFTRILKHGAKDKDD"
     exon            52..247
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /inference="alignment:Splign:2.1.0"
     exon            248..409
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /inference="alignment:Splign:2.1.0"
     exon            410..900
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      880..885
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /note="hexamer: ATTAAA"
     polyA_site      900
                     /gene="MYL12A"
                     /gene_synonym="HEL-S-24; MLC-2B; MLCB; MRCL3; MRLC3;
                     MYL2B"
                     /note="major polyA site"
ORIGIN      
        1 gctgctgagc ggttttgcag actaccatct tccctgcaac tctgaaggat ggacttaacc
       61 accaccatgt cgagcaaaag aacaaagacc aagaccaaga agcgccctca gcgtgcaaca
      121 tccaatgtgt ttgctatgtt tgaccagtca cagattcagg agttcaaaga ggccttcaac
      181 atgattgatc agaacagaga tggtttcatc gacaaggaag atttgcatga tatgcttgct
      241 tcattgggga agaatccaac tgatgagtat ctagatgcca tgatgaatga ggctccaggc
      301 cccatcaatt tcaccatgtt cctcaccatg tttggtgaga agttaaatgg cacagatcct
      361 gaagatgtca tcagaaatgc ctttgcttgc tttgatgaag aagcaactgg caccatacag
      421 gaagattact tgagagagct gctgacaacc atgggggatc ggtttacaga tgaggaagtg
      481 gatgagctgt acagagaagc acctattgat aaaaagggga atttcaatta catcgagttc
      541 acacgcatcc tgaaacatgg agccaaagac aaagatgact gaaataactt caaattccag
      601 ccaaacgttc cttgttgcca ctttgggtat tctgagattt tctcttgcat gcccttagct
      661 ttacagcttt tgcatttcct gttgtattta ttctcagcca ttttgggcat atgtatcttt
      721 ataatcagac tggaaacggg actttctatt aatatcattt tcagaataaa aaataggata
      781 atttaaccta ccagcccttc tcccccaata actgtggtct atacagagtc aatatatttt
      841 ttcagagaaa gttattcgct cgattttttc tgaatcataa ttaaacttta tgataaaata
//
