LOCUS       NM_017533               6016 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens myosin heavy chain 4 (MYH4), mRNA.
ACCESSION   NM_017533
VERSION     NM_017533.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Qiu,X., Chen,D., Li,M., Gao,Y., Liu,F., Zheng,H. and Chen,S.
  TITLE     Transition of myosin heavy chain isoforms in human laryngeal
            abductors following denervation
  JOURNAL   Eur Arch Otorhinolaryngol 272 (10), 2915-2923 (2015)
   PUBMED   26059207
  REMARK    GeneRIF: Transcriptional levels of MHC-I, MHC-IIa, and MHC-IIb in
            denervation groups were significantly down-regulated compared with
            controls
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Smerdu,V. and Cvetko,E.
  TITLE     Myosin heavy chain-2b transcripts and isoform are expressed in
            human laryngeal muscles
  JOURNAL   Cells Tissues Organs 198 (1), 75-86 (2013)
   PUBMED   23796659
  REMARK    GeneRIF: MyHC-2b was always co-expressed with the major MyHC
            isoforms, not only with the fast ones (MyHC-2a and -2x) but with
            the slow isoform (MyHC-1) as well.
REFERENCE   3  (bases 1 to 6016)
  AUTHORS   Bond,J.E., Ho,T.Q., Selim,M.A., Hunter,C.L., Bowers,E.V. and
            Levinson,H.
  TITLE     Temporal spatial expression and function of non-muscle myosin II
            isoforms IIA and IIB in scar remodeling
  JOURNAL   Lab Invest 91 (4), 499-508 (2011)
   PUBMED   21102503
  REMARK    GeneRIF: NMMII and actin isoform expression changes coordinately
            with the remodeling phase of repair, and NMMII is increased as
            matrix stiffness increases. As NMMII expression increases, so does
            the fibroblast contractility.
REFERENCE   4  (bases 1 to 6016)
  AUTHORS   Li,Z.H., Spektor,A., Varlamova,O. and Bresnick,A.R.
  TITLE     Mts1 regulates the assembly of nonmuscle myosin-IIA
  JOURNAL   Biochemistry 42 (48), 14258-14266 (2003)
   PUBMED   14640694
REFERENCE   5  (bases 1 to 6016)
  AUTHORS   Horton,M.J., Brandon,C.A., Morris,T.J., Braun,T.W., Yaw,K.M. and
            Sciote,J.J.
  TITLE     Abundant expression of myosin heavy-chain IIB RNA in a subset of
            human masseter muscle fibres
  JOURNAL   Arch Oral Biol 46 (11), 1039-1050 (2001)
   PUBMED   11543711
REFERENCE   6  (bases 1 to 6016)
  AUTHORS   Ford,H.L., Silver,D.L., Kachar,B., Sellers,J.R. and Zain,S.B.
  TITLE     Effect of Mts1 on the structure and activity of nonmuscle myosin II
  JOURNAL   Biochemistry 36 (51), 16321-16327 (1997)
   PUBMED   9405067
REFERENCE   7  (bases 1 to 6016)
  AUTHORS   Sant'ana Pereira,J.A., Wessels,A., Nijtmans,L., Moorman,A.F. and
            Sargeant,A.J.
  TITLE     New method for the accurate characterization of single human
            skeletal muscle fibres demonstrates a relation between mATPase and
            MyHC expression in pure and hybrid fibre types
  JOURNAL   J Muscle Res Cell Motil 16 (1), 21-34 (1995)
   PUBMED   7751402
REFERENCE   8  (bases 1 to 6016)
  AUTHORS   Larsson,L. and Moss,R.L.
  TITLE     Maximum velocity of shortening in relation to myosin isoform
            composition in single fibres from human skeletal muscles
  JOURNAL   J Physiol 472, 595-614 (1993)
   PUBMED   8145163
REFERENCE   9  (bases 1 to 6016)
  AUTHORS   Soussi-Yanicostas,N., Whalen,R.G. and Petit,C.
  TITLE     Five skeletal myosin heavy chain genes are organized as a multigene
            complex in the human genome
  JOURNAL   Hum Mol Genet 2 (5), 563-569 (1993)
   PUBMED   8518795
REFERENCE   10 (bases 1 to 6016)
  AUTHORS   Barton,P.J. and Buckingham,M.E.
  TITLE     The myosin alkali light chain proteins and their genes
  JOURNAL   Biochem J 231 (2), 249-261 (1985)
   PUBMED   3904738
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF111783.2 and AC005323.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 21, 2006 this sequence version replaced NM_017533.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF111783.2 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1968540,
                                           SAMEA1968968 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000255381.2/ ENSP00000255381.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AF111783.2         1-87
            88-245              AC005323.1         59016-59173         c
            246-1385            AF111783.2         246-1385
            1386-1526           AC005323.1         52357-52497         c
            1527-2444           AF111783.2         1527-2444
            2445-2502           AC005323.1         47389-47446         c
            2503-3428           AF111783.2         2503-3428
            3429-3459           AC005323.1         44820-44850         c
            3460-3849           AC005323.1         44345-44734         c
            3850-3921           AC005323.1         43785-43856         c
            3922-5133           AF111783.2         3922-5133
            5134-5280           AC005323.1         39417-39563         c
            5281-5515           AF111783.2         5281-5515
            5516-5577           AC005323.1         37380-37441         c
            5578-5841           AF111783.2         5578-5841
            5842-6016           AC005323.1         35694-35868         c
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..6016
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /note="myosin heavy chain 4"
                     /db_xref="GeneID:4622"
                     /db_xref="HGNC:HGNC:7574"
                     /db_xref="MIM:160742"
     exon            1..23
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            24..72
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            73..315
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..5931
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /note="myosin, heavy polypeptide 4, skeletal muscle;
                     myosin heavy chain 2b; myosin, heavy chain 4, skeletal
                     muscle; myosin heavy chain IIb; myosin heavy chain,
                     skeletal muscle, fetal"
                     /codon_start=1
                     /product="myosin-4"
                     /protein_id="NP_060003.2"
                     /db_xref="CCDS:CCDS11154.1"
                     /db_xref="GeneID:4622"
                     /db_xref="HGNC:HGNC:7574"
                     /db_xref="MIM:160742"
                     /translation="MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPK
                     ESYVKAIVQSREGGKVTAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAV
                     LYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISD
                     NAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGT
                     LEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRV
                     TFQLKAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMA
                     TDSAVDILGFTADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTS
                     LNSADLLKSLCYPRVKVGNEFVTKGQTVQQVYNAVGALAKAIYEKMFLWMVTRINQQL
                     DTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEG
                     IEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNF
                     QKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLF
                     SGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNET
                     KTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFI
                     DSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAICRG
                     FLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANM
                     KEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCDQLIK
                     TKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEK
                     HATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTK
                     LEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKE
                     FEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRE
                     LEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKHADSV
                     AELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQLSE
                     IKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELK
                     RQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTK
                     YETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDV
                     ERSNAACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEE
                     SLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAE
                     ASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIR
                     SRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQ
                     DDLKEQLAMVERRANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTS
                     LINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLE
                     RMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVK
                     GLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKF
                     RKLQHELEEAKERADIAESQVNKLRVKSREVHTKVISEE"
     exon            316..459
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            460..616
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            617..644
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            645..759
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            760..852
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            853..916
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1015
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1119
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1258
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1377
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1527
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1698
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..2008
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2079
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            2080..2167
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2285
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2409
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            2410..2546
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2802
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..3045
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            3046..3222
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            3223..3368
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            3369..3459
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3849
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            3850..3976
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            3977..4095
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            4096..4292
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            4293..4476
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            4477..4642
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            4643..4767
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            4768..5076
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            5077..5280
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            5281..5406
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            5407..5577
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            5578..5682
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            5683..5778
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
     exon            5779..6016
                     /gene="MYH4"
                     /gene_synonym="MYH2B; MyHC-2B; MyHC-IIb"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atccttcctc aaaattcttg aagtagttgt ctgctttgag cctgccacct tcttcatctg
       61 ataatacaag aggtatacct agtccagtac tgccatcaat aacctgcagc catgagttct
      121 gactctgaga tggccatttt tggggaggct gctcctttcc tccgaaagtc tgaaaaggag
      181 cgaattgaag ctcagaacaa gccttttgat gccaagacat cagtctttgt ggtggaccct
      241 aaggagtcct acgtgaaagc aatagtgcag agcagggaag gggggaaggt gacagccaag
      301 accgaagctg gagctactgt aactgtgaaa gaagaccaag tcttctccat gaaccctccc
      361 aaatatgaca agatcgagga catggccatg atgactcacc tgcatgagcc tgctgtgctg
      421 tataacctca aagagcgtta cgcagcctgg atgatctaca cctactcggg cctcttctgt
      481 gtcaccgtca acccctacaa gtggctgccg gtgtacaacc ctgaggtggt gacagcctac
      541 cgaggcaaaa agcgccagga ggccccaccc catatcttct ccatctctga caatgcctat
      601 cagttcatgc taactgatcg tgaaaaccag tcaatcttga ttactggaga atctggtgca
      661 gggaagactg tgaacacgaa gcgtgtcatc cagtactttg caacaattgc agttactgga
      721 gagaagaaaa aagaggaacc tgcctctggc aaaatgcagg ggacccttga agatcaaatc
      781 atcagtgcta accccctact ggaagccttc ggcaatgcca agaccgtgag gaatgacaac
      841 tcctctcgct ttggtaaatt catcaggatc cattttggtg ccacaggcaa actggcttct
      901 gcagatattg aaacatatct gctagagaag tcccgagtta cttttcagct aaaggctgaa
      961 agaagctacc acatatttta tcaaatcctg tccaataaga aaccagagct cattgaaatg
     1021 cttctgatca ccaccaaccc atatgacttc gcatttgtca gccaagggga aattactgtg
     1081 cccagcattg atgaccagga agagctgatg gccacagata gtgctgtgga catcctgggt
     1141 ttcactgctg atgaaaaggt ggccatttac aagctcactg gagccgtgat gcattatggg
     1201 aacatgaaat tcaagcaaaa gcaaagggaa gagcaggcag agccagatgg cacggaagtt
     1261 gctgacaaag ctgcttatct gacaagtctg aactctgctg acctgctcaa atctctctgc
     1321 tatcccagag tcaaggtcgg caatgagttc gtaaccaaag gccagactgt gcagcaggtg
     1381 tacaatgcag tgggtgctct ggccaaagcc atctacgaga agatgttcct gtggatggtc
     1441 acccgcatca accagcagct ggacaccaag cagcccaggc agtacttcat cggggtcttg
     1501 gacattgctg gctttgagat ctttgatttc aacagcctgg agcagctgtg catcaacttc
     1561 accaacgaga aactgcaaca gtttttcaac caccacatgt tcgtgctgga gcaggaagag
     1621 tacaagaagg aaggcatcga gtgggagttc attgacttcg ggatggacct ggctgcctgc
     1681 atcgagctca tcgagaagcc tatgggcatc ttctccatcc tagaagagga gtgcatgttc
     1741 cccaaggcaa cagacacctc cttcaagaac aagctgtatg aacaacatct tggaaaatcc
     1801 aacaacttcc agaagcccaa gcctgccaaa ggcaagcctg aggctcactt ctcactggtg
     1861 cactatgccg gcaccgtgga ctacaacatc gccggctggc tggacaaaaa caaggacccc
     1921 ctgaatgaga ctgtggtggg gctgtaccag aagtctgcaa tgaagactct ggctttcctc
     1981 ttctctgggg cacaaactgc tgaagcagag ggtggtggtg gaaagaaagg tggcaaaaag
     2041 aagggttctt ctttccagac agtgtcagct cttttcaggg agaatttgaa taagctgatg
     2101 accaacttga ggagcactca cccccacttt gtgcggtgca tcatccccaa tgaaactaaa
     2161 actcctggtg ccatggagca tgagcttgtc ctgcatcagc tgaggtgtaa cggtgtgctg
     2221 gaaggcatcc gcatctgcag gaaaggcttc ccaagcagaa tcctttatgc agacttcaaa
     2281 cagagataca aggttctaaa tgcgagtgct atcccagagg gtcagttcat tgacagcaag
     2341 aaggcttctg agaaacttct agggtctatt gaaattgacc acacccagta caaattcggt
     2401 cataccaagg ttttcttcaa agctggcctg ctgggaactc tagaggaaat gcgagatgaa
     2461 aagctagctc aactcatcac gcgcactcaa gccatatgca gagggttcct gatgagagtg
     2521 gagttcagaa agatgatgga gaggagagag tccatcttct gcattcagta caacatccgt
     2581 gctttcatga atgtgaagca ctggccctgg atgaagctgt atttcaagat caagcccctc
     2641 ctcaagagtg cagagacaga gaaggagatg gccaacatga aggaagaatt tgagaaaacc
     2701 aaagaagagc tggctaagac agaggcaaaa aggaaagaac tagaagaaaa gatggtgacg
     2761 ctaatgcaag agaaaaatga cttacaactc caagttcaag ctgaagcaga tgccttggct
     2821 gatgcagagg aaagatgtga tcagttgatt aaaaccaaaa tccaacttga ggccaaaatc
     2881 aaagaggtaa ctgaaagagc tgaggatgag gaagagatca atgctgagct gacagccaag
     2941 aagaggaaac tggaggatga atgttcagag ctcaagaaag acattgatga ccttgagctg
     3001 acactggcca aggttgagaa ggagaaacat gccacagaga acaaggtgaa aaacctcaca
     3061 gaagagatgg caggtctgga tgaaaccatt gctaagctga ccaaggagaa gaaggctctc
     3121 caggaggccc accagcagac cctggatgac ctgcagatgg aggaggacaa agtcaacacc
     3181 ctgaccaaag ctaaaaccaa gctagaacag caagtggacg atcttgaagg atctctggaa
     3241 caagaaaaga aactttgcat ggacttagaa agagccaaga gaaaactgga gggtgaccta
     3301 aaattggccc aagaatccac aatggataca gaaaatgaca aacagcaact taatgagaaa
     3361 ctcaaaaaga aagagtttga aatgagcaat ctgcaaggca agattgaaga tgaacaagcc
     3421 cttgcaatac agctacaaaa gaagatcaaa gaattacagg cccgcattga ggagctggag
     3481 gaggaaatcg aggcagagcg ggcctcccgg gccaaagcag agaagcagcg ctctgacctc
     3541 tcccgggagc tggaggagat cagtgagagg ctggaagaag ccggtggggc cacttcagcc
     3601 cagattgaga tgaacaagaa gcgggaggct gagttccaga aaatgcgcag ggacctggaa
     3661 gagtccaccc tgcagcacga agccacggca gctgctcttc ggaagaagca cgcagatagt
     3721 gtggctgagc ttggggagca gattgacagc cttcagcggg tcaagcagaa gctggagaag
     3781 gaaaagagtg agctgaagat ggagatcaat gaccttgcta gtaacatgga gactgtctcc
     3841 aaagccaagg caaactttga gaaaatgtgc cgcaccctag aggaccagct tagtgaaata
     3901 aaaacaaagg aagaagagca acaacgctta ataaatgagt tgtcagccca gaaggcacgt
     3961 ttacacacag aatcaggtga gttttcacga cagctagatg aaaaagatgc tatggtttct
     4021 cagctatccc gaggcaaaca agcatttaca caacagattg aagaattaaa gaggcagcta
     4081 gaagaggaga ctaaggccaa gagcactctg gcccatgccc tgcagtcagc ccgccatgac
     4141 tgtgacctgc tgcgggaaca gtatgaggag gagcaggaag ccaaggctga gctgcagagg
     4201 ggaatgtcca aggccaacag tgaggttgcc cagtggagga ccaagtacga gacggacgcc
     4261 atccagcgca cagaggagct ggaggaggcc aagaagaagc tagcccagcg tctgcaggat
     4321 gcagaagaac atgtagaagc tgtgaattcc aaatgtgctt ctcttgaaaa gacaaagcag
     4381 aggctacaga atgaagtaga ggacctcatg attgatgtgg aacgatctaa tgctgcctgc
     4441 atagctctcg ataagaagca aagaaacttt gacaaggttc tggcagaatg gaaacagaag
     4501 tatgaggaaa ctcaggctga acttgaggcc tcccagaagg agtcgcgttc tctcagcact
     4561 gagctgttca aggtgaagaa tgcctacgag gaatccctgg atcatcttga aactctaaag
     4621 cgagagaata agaacttaca acaggagatt tctgacctga cagagcaaat tgcagagggt
     4681 ggaaagcata tccatgaact ggagaaagta aagaaacaac ttgatcatga gaagagtgaa
     4741 ctacagactt ccctagagga agcagaggca tctcttgagc atgaagaagg caaaattctt
     4801 cgcattcaac ttgagctaaa tcaggtgaaa tctgagattg accgaaaaat tgctgaaaaa
     4861 gatgaagaac tcgatcagct aaagaggaac catctcagag ttgtggagtc aatgcagagt
     4921 acactggatg ctgagatcag gagcagaaat gatgctctga ggatcaagaa gaagatggag
     4981 ggagatctta atgaaatgga aatccagctg aaccatgcca accgccaggc tgctgaggca
     5041 ctaaggaatc ttagaaacac acaaggaata ctgaaggaca ctcagctaca tttggatgat
     5101 gccatcagag gccaagatga ccttaaggaa caactggcaa tggttgagcg cagagctaac
     5161 ctgatgcagg ctgaagttga agagctcagg gcatccctgg aacggactga gagaggcagg
     5221 aaaatggcag agcaagagct tctggatgcc agtgaacgtg tgcaacttct gcacactcag
     5281 aacaccagcc tgatcaacac caagaagaag ctggaaacag acatttccca aatccaggga
     5341 gagatggagg acatcgtcca ggaagcccgc aatgcagagg agaaggccaa gaaggccatc
     5401 actgatgctg ccatgatggc tgaggagctg aagaaggaac aggacaccag cgcccacctg
     5461 gagcggatga agaagaacat ggagcagacc gtgaaggatc tgcagctccg tctggatgag
     5521 gctgagcagc tggcgctgaa gggtgggaag aagcagatcc agaaactgga ggccagggtg
     5581 agagagcttg aaagtgaggt ggaaagtgaa cagaagcaca atgttgaggc tgtcaagggt
     5641 cttcgcaaac atgagagaag agtgaaggaa ctcacttacc agactgagga ggaccgcaag
     5701 aatattctca ggctgcagga cttggtggac aaattgcaaa ccaaagtcaa agcttacaag
     5761 agacaagctg aagaggctga ggaacaatcc aatgtcaacc ttgccaagtt ccgcaagctc
     5821 cagcacgagc tggaggaggc caaggaacgg gctgacattg ctgagtccca agtcaacaag
     5881 ctgagagtga agagtcggga ggttcacaca aaagtcataa gtgaagagta attcattcta
     5941 atgaaagaaa atgtgaccaa agaaatgcac gaaatgtgaa gttctttgtc actgtcctgt
     6001 atatcaagga aataaa
//
