LOCUS       NM_001256012            7707 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens myosin heavy chain 10 (MYH10), transcript variant 1,
            mRNA.
ACCESSION   NM_001256012
VERSION     NM_001256012.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Yeung,M.C.F., Dermawan,J.K., Liu,A.P.Y., Lam,A.Y.L., Antonescu,C.R.
            and Shek,T.W.H.
  TITLE     Spindle cell neoplasms with novel LTK fusion - Expanding the
            spectrum of kinase fusion-positive soft tissue tumors
  JOURNAL   Genes Chromosomes Cancer 63 (3), e23227 (2024)
   PUBMED   38517106
  REMARK    GeneRIF: Spindle cell neoplasms with novel LTK fusion - Expanding
            the spectrum of kinase fusion-positive soft tissue tumors.
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Yim,Y.I., Pedrosa,A., Wu,X., Chinthalapudi,K., Cheney,R.E. and
            Hammer,J.A.
  TITLE     Mechanisms underlying Myosin 10's contribution to the maintenance
            of mitotic spindle bipolarity
  JOURNAL   Mol Biol Cell 35 (2), ar14 (2024)
   PUBMED   38019611
  REMARK    GeneRIF: Mechanisms underlying Myosin 10's contribution to the
            maintenance of mitotic spindle bipolarity.
REFERENCE   3  (bases 1 to 7707)
  AUTHORS   Liu,L., Chen,C., Liu,P., Li,J., Pang,Z., Zhu,J., Lin,Z., Zhou,H.,
            Xie,Y., Lan,T., Chen,Z.S., Zeng,Z. and Fang,W.
  TITLE     MYH10 Combines with MYH9 to Recruit USP45 by Deubiquitinating Snail
            and Promotes Serous Ovarian Cancer Carcinogenesis, Progression, and
            Cisplatin Resistance
  JOURNAL   Adv Sci (Weinh) 10 (14), e2203423 (2023)
   PUBMED   36929633
  REMARK    GeneRIF: MYH10 Combines with MYH9 to Recruit USP45 by
            Deubiquitinating Snail and Promotes Serous Ovarian Cancer
            Carcinogenesis, Progression, and Cisplatin Resistance.
REFERENCE   4  (bases 1 to 7707)
  AUTHORS   Holtz,A.M., VanCoillie,R., Vansickle,E.A., Carere,D.A., Withrow,K.,
            Torti,E., Juusola,J., Millan,F., Person,R., Guillen Sacoto,M.J.,
            Si,Y., Wentzensen,I.M., Pugh,J., Vasileiou,G., Rieger,M., Reis,A.,
            Argilli,E., Sherr,E.H., Aldinger,K.A., Dobyns,W.B., Brunet,T.,
            Hoefele,J., Wagner,M., Haber,B., Kotzaeridou,U., Keren,B.,
            Heron,D., Mignot,C., Heide,S., Courtin,T., Buratti,J.,
            Murugasen,S., Donald,K.A., O'Heir,E., Moody,S., Kim,K.H.,
            Burton,B.K., Yoon,G., Campo,M.D., Masser-Frye,D., Kozenko,M.,
            Parkinson,C., Sell,S.L., Gordon,P.L., Prokop,J.W., Karaa,A.,
            Bupp,C. and Raby,B.A.
  TITLE     Heterozygous variants in MYH10 associated with neurodevelopmental
            disorders and congenital anomalies with evidence for primary
            cilia-dependent defects in Hedgehog signaling
  JOURNAL   Genet Med 24 (10), 2065-2078 (2022)
   PUBMED   35980381
  REMARK    GeneRIF: Heterozygous variants in MYH10 associated with
            neurodevelopmental disorders and congenital anomalies with evidence
            for primary cilia-dependent defects in Hedgehog signaling.
REFERENCE   5  (bases 1 to 7707)
  AUTHORS   Phillips,C.L., Yamakawa,K. and Adelstein,R.S.
  TITLE     Cloning of the cDNA encoding human nonmuscle myosin heavy chain-B
            and analysis of human tissues with isoform-specific antibodies
  JOURNAL   J Muscle Res Cell Motil 16 (4), 379-389 (1995)
   PUBMED   7499478
REFERENCE   6  (bases 1 to 7707)
  AUTHORS   Strand,D., Unger,S., Corvi,R., Hartenstein,K., Schenkel,H.,
            Kalmes,A., Merdes,G., Neumann,B., Krieg-Schneider,F., Coy,J.F. et
            al.
  TITLE     A human homologue of the Drosophila tumour suppressor gene l(2)gl
            maps to 17p11.2-12 and codes for a cytoskeletal protein that
            associates with nonmuscle myosin II heavy chain
  JOURNAL   Oncogene 11 (2), 291-301 (1995)
   PUBMED   7542763
REFERENCE   7  (bases 1 to 7707)
  AUTHORS   Itoh,K. and Adelstein,R.S.
  TITLE     Neuronal cell expression of inserted isoforms of vertebrate
            nonmuscle myosin heavy chain II-B
  JOURNAL   J Biol Chem 270 (24), 14533-14540 (1995)
   PUBMED   7782316
REFERENCE   8  (bases 1 to 7707)
  AUTHORS   Maupin,P., Phillips,C.L., Adelstein,R.S. and Pollard,T.D.
  TITLE     Differential localization of myosin-II isozymes in human cultured
            cells and blood cells
  JOURNAL   J Cell Sci 107 (Pt 11), 3077-3090 (1994)
   PUBMED   7699007
REFERENCE   9  (bases 1 to 7707)
  AUTHORS   Aikawa,M., Sivam,P.N., Kuro-o,M., Kimura,K., Nakahara,K.,
            Takewaki,S., Ueda,M., Yamaguchi,H., Yazaki,Y., Periasamy,M. et al.
  TITLE     Human smooth muscle myosin heavy chain isoforms as molecular
            markers for vascular development and atherosclerosis
  JOURNAL   Circ Res 73 (6), 1000-1012 (1993)
   PUBMED   7916668
REFERENCE   10 (bases 1 to 7707)
  AUTHORS   Simons,M., Wang,M., McBride,O.W., Kawamoto,S., Yamakawa,K.,
            Gdula,D., Adelstein,R.S. and Weir,L.
  TITLE     Human nonmuscle myosin heavy chains are encoded by two genes
            located on different chromosomes
  JOURNAL   Circ Res 69 (2), 530-539 (1991)
   PUBMED   1860190
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011061.13, AC025518.8 and
            AC026130.15.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 30, 2019 this sequence version replaced NM_001256012.2.
            
            Summary: This gene encodes a member of the myosin superfamily. The
            protein represents a conventional non-muscle myosin; it should not
            be confused with the unconventional myosin-10 (MYO10). Myosins are
            actin-dependent motor proteins with diverse functions including
            regulation of cytokinesis, cell motility, and cell polarity.
            Mutations in this gene have been associated with May-Hegglin
            anomaly and developmental defects in brain and heart. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Dec 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB210026.1, SRR1803614.178881.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360416.8/ ENSP00000353590.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                AC011061.13        99577-99648
            73-448              AC011061.13        107025-107400
            449-605             AC011061.13        125320-125476
            606-633             AC025518.8         13258-13285
            634-736             AC025518.8         25028-25130
            737-766             AC025518.8         25694-25723
            767-859             AC025518.8         32554-32646
            860-923             AC025518.8         48348-48411
            924-1022            AC025518.8         50222-50320
            1023-1166           AC025518.8         53579-53722
            1167-1262           AC025518.8         53958-54053
            1263-1381           AC025518.8         55704-55822
            1382-1534           AC025518.8         56766-56918
            1535-1708           AC025518.8         60086-60259
            1709-1882           AC026130.15        2671-2844
            1883-1997           AC026130.15        3101-3215
            1998-2060           AC026130.15        7917-7979
            2061-2254           AC026130.15        17317-17510
            2255-2376           AC026130.15        17603-17724
            2377-2446           AC026130.15        19652-19721
            2447-2607           AC026130.15        19811-19971
            2608-2716           AC026130.15        24708-24816
            2717-2848           AC026130.15        24933-25064
            2849-3055           AC026130.15        25945-26151
            3056-3193           AC026130.15        28653-28790
            3194-3317           AC026130.15        29925-30048
            3318-3489           AC026130.15        32113-32284
            3490-3702           AC026130.15        33696-33908
            3703-3847           AC026130.15        37632-37776
            3848-4054           AC026130.15        39126-39332
            4055-4159           AC026130.15        43361-43465
            4160-4312           AC026130.15        44717-44869
            4313-4561           AC026130.15        45578-45826
            4562-4774           AC026130.15        46093-46305
            4775-4987           AC026130.15        48050-48262
            4988-5149           AC026130.15        51008-51169
            5150-5278           AC026130.15        54336-54464
            5279-5367           AC026130.15        57192-57280
            5368-5491           AC026130.15        58077-58200
            5492-5700           AC026130.15        58284-58492
            5701-5809           AC026130.15        60156-60264
            5810-5982           AC026130.15        61554-61726
            5983-7707           AC026130.15        62654-64378
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..7707
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /note="myosin heavy chain 10"
                     /db_xref="GeneID:4628"
                     /db_xref="HGNC:HGNC:7568"
                     /db_xref="MIM:160776"
     exon            1..72
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            73..448
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..6127
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myosin, heavy polypeptide 10, non-muscle; myosin heavy
                     chain, nonmuscle type B; nonmuscle myosin heavy chain IIB;
                     nonmuscle myosin II heavy chain-B; myosin, heavy chain 10,
                     non-muscle"
                     /codon_start=1
                     /product="myosin-10 isoform 1"
                     /protein_id="NP_001242941.1"
                     /db_xref="CCDS:CCDS58515.1"
                     /db_xref="GeneID:4628"
                     /db_xref="HGNC:HGNC:7568"
                     /db_xref="MIM:160776"
                     /translation="MAQRTGLEDPERYLFVDRAVIYNPATQADWTAKKLVWIPSERHG
                     FEAASIKEERGDEVMVELAENGKKAMVNKDDIQKMNPPKFSKVEDMAELTCLNEASVL
                     HNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYSENIIEMYRGKKRHEMPPHIYAISES
                     AYRCMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKDHNIPQESPKPV
                     KHQGELERQLLQANPILESFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLL
                     EKSRAVRQAKDERTFHIFYQLLSGAGEHLKSDLLLEGFNNYRFLSNGYIPIPGQQDKD
                     NFQETMEAMHIMGFSHEEILSMLKVVSSVLQFGNISFKKERNTDQASMPENTVAQKLC
                     HLLGMNVMEFTRAILTPRIKVGRDYVQKAQTKEQADFAVEALAKATYERLFRWLVHRI
                     NKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFILEQEE
                     YQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLALLDEECWFPKATDKTFVEKLVQE
                     QGSHSKFQKPRQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSSDRF
                     VAELWKDEIQNIQRASFYDSVSGLHEPPVDRIVGLDQVTGMTETAFGSAYKTKKGMFR
                     TVGQLYKESLTKLMATLRNTNPNFVRCIIPNHEKRAGKLDPHLVLDQLRCNGVLEGIR
                     ICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACERMIRALELDPNLYRIGQSK
                     IFFRAGVLAHLEEERDLKITDIIIFFQAVCRGYLARKAFAKKQQQLSALKVLQRNCAA
                     YLKLRHWQWWRVFTKVKPLLQVTRQEEELQAKDEELLKVKEKQTKVEGELEEMERKHQ
                     QLLEEKNILAEQLQAETELFAEAEEMRARLAAKKQELEEILHDLESRVEEEEERNQIL
                     QNEKKKMQAHIQDLEEQLDEEEGARQKLQLEKVTAEAKIKKMEEEILLLEDQNSKFIK
                     EKKLMEDRIAECSSQLAEEEEKAKNLAKIRNKQEVMISDLEERLKKEEKTRQELEKAK
                     RKLDGETTDLQDQIAELQAQIDELKLQLAKKEEELQGALARGDDETLHKNNALKVVRE
                     LQAQIAELQEDFESEKASRNKAEKQKRDLSEELEALKTELEDTLDTTAAQQELRTKRE
                     QEVAELKKALEEETKNHEAQIQDMRQRHATALEELSEQLEQAKRFKANLEKNKQGLET
                     DNKELACEVKVLQQVKAESEHKRKKLDAQVQELHAKVSEGDRLRVELAEKASKLQNEL
                     DNVSTLLEEAEKKGIKFAKDAASLESQLQDTQELLQEETRQKLNLSSRIRQLEEEKNS
                     LQEQQEEEEEARKNLEKQVLALQSQLADTKKKVDDDLGTIESLEEAKKKLLKDAEALS
                     QRLEEKALAYDKLEKTKNRLQQELDDLTVDLDHQRQVASNLEKKQKKFDQLLAEEKSI
                     SARYAEERDRAEAEAREKETKALSLARALEEALEAKEEFERQNKQLRADMEDLMSSKD
                     DVGKNVHELEKSKRALEQQVEEMRTQLEELEDELQATEDAKLRLEVNMQAMKAQFERD
                     LQTRDEQNEEKKRLLIKQVRELEAELEDERKQRALAVASKKKMEIDLKDLEAQIEAAN
                     KARDEVIKQLRKLQAQMKDYQRELEEARASRDEIFAQSKESEKKLKSLEAEILQLQEE
                     LASSERARRHAEQERDELADEITNSASGKSALLDEKRRLEARIAQLEEELEEEQSNME
                     LLNDRFRKTTLQVDTLNAELAAERSAAQKSDNARQQLERQNKELKAKLQELEGAVKSK
                     FKATISALEAKIGQLEEQLEQEAKERAAANKLVRRTEKKLKEIFMQVEDERRHADQYK
                     EQMEKANARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNR
                     LRRGGPISFSSSRSGRRQLHLEGASLELSDDDTESKTSDVNETQPPQSE"
     exon            449..605
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            606..633
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            634..736
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            737..766
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            767..859
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            860..923
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1022
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1166
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1262
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1381
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1534
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1535..1708
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1882
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1997
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2060
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2254
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2376
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            2377..2446
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..2607
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2716
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            2717..2848
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            2849..3055
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            3056..3193
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            3194..3317
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            3318..3489
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            3490..3702
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            3703..3847
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            3848..4054
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            4055..4159
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            4160..4312
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            4313..4561
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            4562..4774
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            4775..4987
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            4988..5149
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            5150..5278
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            5279..5367
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            5368..5491
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            5492..5700
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            5701..5809
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            5810..5982
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     exon            5983..7707
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7615..7620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /note="hexamer: AATAAA"
     polyA_site      7637
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /note="major polyA site"
     regulatory      7682..7687
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /note="hexamer: AATAAA"
     polyA_site      7707
                     /gene="MYH10"
                     /gene_synonym="NMMHC-IIB; NMMHCB"
                     /note="major polyA site"
ORIGIN      
        1 gtttgggact gaggcgctgg atctgtggtc gcggctgggg acgtgcgccc gcgccaccat
       61 cttcggctga agaggcaatt gcttttggat cgttccattt acaatggcgc agagaactgg
      121 actcgaggat ccagagaggt atctctttgt ggacagggct gtcatctaca accctgccac
      181 tcaagctgat tggacagcta aaaagctagt gtggattcca tcagaacgcc atggttttga
      241 ggcagctagt atcaaagaag aacggggaga tgaagttatg gtggagttgg cagagaatgg
      301 aaagaaagca atggtcaaca aagatgatat tcagaagatg aacccaccta agttttccaa
      361 ggtggaggat atggcagaat tgacatgctt gaatgaagct tccgttttac ataatctgaa
      421 ggatcgctac tattcaggac taatctatac ttattctgga ctcttctgtg tagttataaa
      481 cccttacaag aatcttccaa tttactctga gaatattatt gaaatgtaca gagggaagaa
      541 gcgtcatgag atgcctccac acatctatgc tatatctgaa tctgcttaca gatgcatgct
      601 tcaagatcgt gaggaccagt caattctttg cacgggtgag tcaggtgctg ggaagacaga
      661 aaatacaaag aaagttattc agtaccttgc ccatgttgct tcttcacata aaggaagaaa
      721 ggaccataat attcctcagg aatcgcctaa accagtgaaa caccaggggg aacttgaacg
      781 gcagcttttg caagcaaatc caattctgga atcatttgga aatgcgaaga ctgtgaaaaa
      841 tgataactca tctcgttttg gcaaatttat tcggatcaac tttgatgtaa ctggctatat
      901 cgttggggcc aacattgaaa cataccttct ggaaaagtct cgtgctgttc gtcaagcaaa
      961 agatgaacgt acttttcata tcttttacca gttgttatct ggagcaggag aacacctaaa
     1021 gtctgatttg cttcttgaag gatttaataa ctacaggttt ctctccaatg gctatattcc
     1081 tattccggga cagcaagaca aagataattt ccaggagacc atggaagcaa tgcacataat
     1141 gggcttctcc catgaagaga ttctgtcaat gcttaaagta gtatcttcag tgctacagtt
     1201 tggaaatatt tctttcaaaa aggagagaaa tactgatcaa gcttccatgc cagaaaatac
     1261 agttgcgcag aagctctgcc atcttcttgg gatgaatgtg atggagttta ctcgggccat
     1321 cctgactccc cggatcaagg tcggccgaga ctatgtgcaa aaagcccaga ccaaagaaca
     1381 ggcagatttt gcagtagaag cattggcaaa agctacctat gagcggctct ttcgctggct
     1441 cgttcatcgc atcaataaag ctctggatag gaccaaacgt cagggagcat ctttcattgg
     1501 aatcctggat attgctggat ttgaaatttt tgagctgaac tcctttgaac aactttgcat
     1561 caactacacc aatgagaagc tgcagcagct gttcaaccac accatgttta tcctagaaca
     1621 agaggaatac cagcgcgaag gcatcgagtg gaacttcatc gatttcgggc tggatctgca
     1681 gccatgcatc gacctaatag agagacctgc gaaccctcct ggtgtactgg cccttttgga
     1741 tgaagaatgc tggttcccta aagccacaga taaaaccttt gttgaaaaac tggttcaaga
     1801 gcaaggttcc cactccaagt ttcagaaacc tcgacaatta aaagacaaag ctgatttttg
     1861 cattatacat tatgcaggga aggtggacta taaggcagat gagtggctga tgaagaatat
     1921 ggaccccctg aatgacaacg tggccaccct tttgcaccag tcatcagaca gatttgtggc
     1981 agagctttgg aaagatgaga ttcagaatat tcagagagct tctttctatg acagtgtttc
     2041 tggtcttcat gagccaccag tggaccgtat cgtgggtctg gatcaagtca ctggtatgac
     2101 tgagacagct tttggctccg catataaaac caagaagggc atgtttcgta ccgttgggca
     2161 actctacaaa gaatctctca ccaagctgat ggcaactctc cgaaacacca accctaactt
     2221 tgttcgttgt atcattccaa atcacgagaa gagggctgga aaattggatc cacacctagt
     2281 cctagatcag cttcgctgta acggtgtcct ggaagggatc cgaatctgtc gccagggctt
     2341 ccctaaccga atagttttcc aggaattcag acagagatat gagatcctaa ctccaaatgc
     2401 tattcctaaa ggttttatgg atggtaaaca ggcctgtgaa cgaatgatcc gggctttaga
     2461 attggaccca aacttgtaca gaattggaca gagcaagata tttttcagag ctggagttct
     2521 ggcacactta gaggaagaaa gagatttaaa aatcaccgat atcattatct tcttccaggc
     2581 cgtttgcaga ggttacctgg ccagaaaggc ctttgccaag aagcagcagc aactaagtgc
     2641 cttaaaggtc ttgcagcgga actgtgccgc gtacctgaaa ttacggcact ggcagtggtg
     2701 gcgagtcttc acaaaggtga agccgcttct acaagtgact cgccaggagg aagaacttca
     2761 ggccaaagat gaagagctgt tgaaggtgaa ggagaagcag acgaaggtgg aaggagagct
     2821 ggaggagatg gagcggaagc accagcagct tttagaagag aagaatatcc ttgcagaaca
     2881 actacaagca gagactgagc tctttgctga agcagaagag atgagggcaa gacttgctgc
     2941 taaaaagcag gaattagaag agattctaca tgacttggag tctagggttg aagaagaaga
     3001 agaaagaaac caaatcctcc aaaatgaaaa gaaaaaaatg caagcacata ttcaggacct
     3061 ggaagaacag ctagacgagg aggaaggggc tcggcaaaag ctgcagctgg aaaaggtgac
     3121 agcagaggcc aagatcaaga agatggaaga ggagattctg cttctcgagg accaaaattc
     3181 caagttcatc aaagaaaaga aactcatgga agatcgcatt gctgagtgtt cctctcagct
     3241 ggctgaagag gaagaaaagg cgaaaaactt ggccaaaatc aggaataagc aagaagtgat
     3301 gatctcagat ttagaagaac gcttaaagaa ggaagaaaag actcgtcagg aactggaaaa
     3361 ggccaaaaga aaactcgacg gggagacgac cgacctgcag gaccagatcg cagagctgca
     3421 ggcgcagatt gatgagctca agctgcagct ggccaagaag gaggaggagc tgcagggcgc
     3481 actggccaga ggtgatgatg aaacactcca taagaacaat gcccttaaag ttgtgcgaga
     3541 gctacaagcc caaattgctg aacttcagga agactttgaa tccgagaagg cttcacggaa
     3601 caaggccgaa aagcagaaaa gggacttgag tgaggaactg gaagctctga aaacagagct
     3661 ggaggacacg ctggacacca cggcagccca gcaggaacta cgtacaaaac gtgaacaaga
     3721 agtggcagag ctgaagaaag ctcttgagga ggaaactaag aaccatgaag ctcaaatcca
     3781 ggacatgaga caaagacacg caacagccct ggaggagctc tcagagcagc tggaacaggc
     3841 caagcggttc aaagcaaatc tagagaagaa caagcagggc ctggagacag ataacaagga
     3901 gctggcgtgt gaggtgaagg tcctgcagca ggtcaaggct gagtctgagc acaagaggaa
     3961 gaagctcgac gcgcaggtcc aggagctcca tgccaaggtc tctgaaggcg acaggctcag
     4021 ggtggagctg gcggagaaag caagtaagct gcagaatgag ctagataatg tctccaccct
     4081 tctggaagaa gcagagaaga agggtattaa atttgctaag gatgcagcta gtcttgagtc
     4141 tcaactacag gatacacagg agcttcttca ggaggagaca cgccagaaac taaacctgag
     4201 cagtcggatc cggcagctgg aagaggagaa gaacagtctt caggagcagc aggaggagga
     4261 ggaggaggcc aggaagaacc tggagaagca agtgctggcc ctgcagtccc agttggctga
     4321 taccaagaag aaagtagatg acgacctggg aacaattgaa agtctggaag aagccaagaa
     4381 gaagcttctg aaggacgcgg aggccctgag ccagcgcctg gaggagaagg cactggcgta
     4441 tgacaaactg gagaagacca agaaccgcct gcagcaggag ctggacgacc tcacggtgga
     4501 cctggaccac cagcgccagg tcgcctccaa cttggagaag aagcagaaga agtttgacca
     4561 gctgttagca gaagagaaga gcatctctgc tcgctatgcc gaagagcggg accgggccga
     4621 agccgaggcc agagagaaag aaaccaaagc cctgtcactg gcccgggccc tcgaggaagc
     4681 cctggaggcc aaggaggagt ttgagaggca gaacaagcag ctccgagcag acatggaaga
     4741 cctcatgagc tccaaagatg atgtgggaaa aaacgttcac gaacttgaaa aatccaaacg
     4801 ggccctagag cagcaggtgg aggaaatgag gacccagctg gaggagctgg aagacgaact
     4861 ccaggccacg gaagatgcca agcttcgtct ggaggtcaac atgcaggcca tgaaggcgca
     4921 gttcgagaga gacctgcaaa ccagggatga gcagaatgaa gagaagaagc ggctgctgat
     4981 caaacaggtg cgggagctcg aggcggagct ggaggatgag aggaaacagc gggcgcttgc
     5041 tgtagcttca aagaaaaaga tggagataga cctgaaggac ctcgaagccc aaatcgaggc
     5101 tgcgaacaaa gctcgggatg aggtgattaa gcagctccgc aagctccagg ctcagatgaa
     5161 ggattaccaa cgtgaattag aagaagctcg tgcatccaga gatgagattt ttgctcaatc
     5221 caaagagagt gaaaagaaat tgaagagtct ggaagcagaa atccttcaat tgcaggagga
     5281 acttgcctca tctgagcgag cccgccgaca cgccgagcag gagagagatg agctggcgga
     5341 cgagatcacc aacagcgcct ctggcaagtc cgcgctgctg gatgagaagc ggcgtctgga
     5401 agctcggatc gcacagctgg aggaggagct ggaagaggag cagagcaaca tggagctgct
     5461 caacgaccgc ttccgcaaga ccactctaca ggtggacaca ctgaacgccg agctagcagc
     5521 cgagcgcagc gccgcccaga agagtgacaa tgcacgccag caactggagc ggcagaacaa
     5581 ggagctgaag gccaagctgc aggaactcga gggtgctgtc aagtctaagt tcaaggccac
     5641 catctcagcc ctggaggcca agattgggca gctggaggag cagcttgagc aggaagccaa
     5701 ggaacgagca gccgccaaca aattagtccg tcgcactgag aagaagctga aagaaatctt
     5761 catgcaggtt gaggatgagc gtcgacacgc ggaccagtat aaagagcaga tggagaaggc
     5821 caacgctcgg atgaagcagc ttaaacgcca gctggaggaa gcagaagaag aagcgacgcg
     5881 tgccaacgca tctcggcgta aactccagcg ggaactggat gatgccaccg aggccaacga
     5941 gggcctgagc cgcgaggtca gcaccctgaa gaaccggctg aggcggggtg gccccatcag
     6001 cttctcttcc agccgatctg gccggcgcca gctgcacctt gaaggagctt ccctggagct
     6061 ctccgacgat gacacagaaa gtaagaccag tgatgtcaac gagacgcagc caccccagtc
     6121 agagtaaagt tgcaggaagc cagaggaggc aatacagtgg gacagttagg aatgcacccg
     6181 gggcctcctg cagatttcgg aaattggcaa gctacgggat tccttcctga aagatcaact
     6241 gtgtcttaag gctctccagc ctatgcatac tgtatcctgc ttcagactta ggtacaattg
     6301 ctcccctttt tatatataga cacacacagg acacatatat taaacagatt gtttcatcat
     6361 tgcatctatt ttccatatag tcatcaagag accattttat aaaacatggt aagacccttt
     6421 ttaaaacaaa ctccaggccc ttggttgcgg gtcgctgggt tattggggca gcgccgtggt
     6481 cgtcactcag tcgctctgca tgctctctgt catacagaca ggtaacctag ttctgtgttc
     6541 acgtggcccc cgactcctca gccacatcaa gtctcctaga ccactgtgga ctctaaactg
     6601 cacttgtctc tctcatttcc ttcaaataat gatcaatgct atttcagtga gcaaactgtg
     6661 aaaggggctt tggaaagagt aggaggggtg ggctggatcg gaagcaacac ccatttgggg
     6721 ttaccatgtc catcccccaa ggggggccct gcccctcgag tcgatggtgt cccgcatcta
     6781 ctcatgtgaa ctggccttgg cgagggctgg tctgtgcata gaagggatag tggccacact
     6841 gcagctgagg ccccaggtgg cagccatgga tcatgtagac ttccagatgg tctcccgaac
     6901 cgcctggctc tgccggcgcc ctcctcacgt caggagcaag cagccgtgga cccctaagcc
     6961 gagctggtgg aaggcccctc cccatcgcca gccgggccct catgctgacc ttgcaaattc
     7021 agccgctgct ttgagcccaa aatgggaata ttggttttgt gtccgaggct tgttccaagt
     7081 ttgtcaatga ggtttatgga gcctccagaa cagatgccat cttcctgaat gttgacatgc
     7141 cagtgggtgt gactccttca tttttccttc tcccttccct ttggacagtg ttacagtgaa
     7201 cacttagcat cctgtttttg gttggtagtt aagcaaactg acattacgga aagtgcctta
     7261 gacactacag tactaagaca atgttgaata tatcattcgc ctctataaca atttaatgta
     7321 ttcagttttg actgtgcttc atatcatgta cctctctagt caaagtggta ttacagacat
     7381 tcagtgacaa tgaatcagtg ttaattctaa atccttgatc ctctgcaatg tgcttgaaaa
     7441 cacaaacctt ttgggttaaa agctttaaca tctattagga agaatttgtc ctgtgggttt
     7501 ggaatcttgg attttccccc tttatgaact gtactggctg ttgaccacca gacacctgac
     7561 cgcaaatatc ttttcttgta ttcccatatt tctagacaat gatttttgta agacaataaa
     7621 tttattcatt atagatattt gcgcctgctc tgtttacttg aagaaaaaag cacccgtgga
     7681 gaataaagag acctcaataa acaagaa
//
