LOCUS       NM_005963               6023 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens myosin heavy chain 1 (MYH1), mRNA.
ACCESSION   NM_005963
VERSION     NM_005963.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Alsaif,H.S., Alshehri,A., Sulaiman,R.A., Al-Hindi,H.,
            Guzman-Vega,F.J., Arold,S.T. and Alkuraya,F.S.
  TITLE     MYH1 is a candidate gene for recurrent rhabdomyolysis in humans
  JOURNAL   Am J Med Genet A 185 (7), 2131-2135 (2021)
   PUBMED   33755318
  REMARK    GeneRIF: MYH1 is a candidate gene for recurrent rhabdomyolysis in
            humans.
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6023)
  AUTHORS   Qiu,X., Chen,D., Li,M., Gao,Y., Liu,F., Zheng,H. and Chen,S.
  TITLE     Transition of myosin heavy chain isoforms in human laryngeal
            abductors following denervation
  JOURNAL   Eur Arch Otorhinolaryngol 272 (10), 2915-2923 (2015)
   PUBMED   26059207
  REMARK    GeneRIF: Transcriptional levels of MHC-I, MHC-IIa, and MHC-IIb in
            denervation groups were significantly down-regulated compared with
            controls
REFERENCE   4  (bases 1 to 6023)
  AUTHORS   Miller,M.S., Bedrin,N.G., Ades,P.A., Palmer,B.M. and Toth,M.J.
  TITLE     Molecular determinants of force production in human skeletal muscle
            fibers: effects of myosin isoform expression and cross-sectional
            area
  JOURNAL   Am J Physiol Cell Physiol 308 (6), C473-C484 (2015)
   PUBMED   25567808
  REMARK    GeneRIF: Myosin isoforms impact single-fiber force generation and
            may lead to alterations in whole skeletal muscle performance.
REFERENCE   5  (bases 1 to 6023)
  AUTHORS   Cvetko,E., Karen,P. and Erzen,I.
  TITLE     Wearing of complete dentures reduces slow fibre and enhances hybrid
            fibre fraction in masseter muscle
  JOURNAL   J Oral Rehabil 39 (8), 608-614 (2012)
   PUBMED   22489880
  REMARK    GeneRIF: adaptive response to reduced masticatory load was lower
            numerical and area proportion of MyHC-1 expressing fibres and
            higher numerical proportion of hybrid fibres in edentulous compared
            with dentate subjects
REFERENCE   6  (bases 1 to 6023)
  AUTHORS   Weiss,A., McDonough,D., Wertman,B., Acakpo-Satchivi,L.,
            Montgomery,K., Kucherlapati,R., Leinwand,L. and Krauter,K.
  TITLE     Organization of human and mouse skeletal myosin heavy chain gene
            clusters is highly conserved
  JOURNAL   Proc Natl Acad Sci U S A 96 (6), 2958-2963 (1999)
   PUBMED   10077619
REFERENCE   7  (bases 1 to 6023)
  AUTHORS   Smerdu,V., Karsch-Mizrachi,I., Campione,M., Leinwand,L. and
            Schiaffino,S.
  TITLE     Type IIx myosin heavy chain transcripts are expressed in type IIb
            fibers of human skeletal muscle
  JOURNAL   Am J Physiol 267 (6 Pt 1), C1723-C1728 (1994)
   PUBMED   7545970
REFERENCE   8  (bases 1 to 6023)
  AUTHORS   Yoon,S.J., Seiler,S.H., Kucherlapati,R. and Leinwand,L.
  TITLE     Organization of the human skeletal myosin heavy chain gene cluster
  JOURNAL   Proc Natl Acad Sci U S A 89 (24), 12078-12082 (1992)
   PUBMED   1465443
REFERENCE   9  (bases 1 to 6023)
  AUTHORS   Saez,L. and Leinwand,L.A.
  TITLE     Characterization of diverse forms of myosin heavy chain expressed
            in adult human skeletal muscle
  JOURNAL   Nucleic Acids Res 14 (7), 2951-2969 (1986)
   PUBMED   2421254
REFERENCE   10 (bases 1 to 6023)
  AUTHORS   Leinwand,L.A., Saez,L., McNally,E. and Nadal-Ginard,B.
  TITLE     Isolation and characterization of human myosin heavy chain genes
  JOURNAL   Proc Natl Acad Sci U S A 80 (12), 3716-3720 (1983)
   PUBMED   6304733
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA826478.1, BC114545.1,
            AF111785.1 and AJ573764.1.
            
            On Nov 22, 2018 this sequence version replaced NM_005963.3.
            
            Summary: Myosin is a major contractile protein which converts
            chemical energy into mechanical energy through the hydrolysis of
            ATP. Myosin is a hexameric protein composed of a pair of myosin
            heavy chains (MYH) and two pairs of nonidentical light chains.
            Myosin heavy chains are encoded by a multigene family. In mammals
            at least 10 different myosin heavy chain (MYH) isoforms have been
            described from striated, smooth, and nonmuscle cells. These
            isoforms show expression that is spatially and temporally regulated
            during development. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF111785.1, EU794637.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1968540,
                                    SAMEA1968968 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000226207.6/ ENSP00000226207.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               DA826478.1         1-103
            104-1125            BC114545.1         10-1031
            1126-1126           AF111785.1         1032-1032
            1127-4684           BC114545.1         1033-4590
            4685-4685           AF111785.1         4591-4591
            4686-5964           BC114545.1         4592-5870
            5965-6023           AJ573764.1         371-429
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.1"
     gene            1..6023
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /note="myosin heavy chain 1"
                     /db_xref="GeneID:4619"
                     /db_xref="HGNC:HGNC:7567"
                     /db_xref="MIM:160730"
     exon            1..23
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            24..54
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            55..298
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..5914
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /note="myosin heavy chain IIx/d; myosin, heavy polypeptide
                     1, skeletal muscle, adult; myHC-IIx/d; myosin heavy chain
                     2x; myosin heavy chain, skeletal muscle, adult 1;
                     epididymis luminal protein 71; myosin, heavy chain 1,
                     skeletal muscle, adult"
                     /codon_start=1
                     /product="myosin-1"
                     /protein_id="NP_005954.3"
                     /db_xref="CCDS:CCDS11155.1"
                     /db_xref="GeneID:4619"
                     /db_xref="HGNC:HGNC:7567"
                     /db_xref="MIM:160730"
                     /translation="MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPK
                     ESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAV
                     LYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISD
                     NAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGT
                     LEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRV
                     TFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMA
                     TDSAIEILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQN
                     LNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNAVGALAKAVYDKMFLWMVTRINQQL
                     DTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEG
                     IEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNF
                     QKPKPAKGKPEAHFSLIHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLALLF
                     VGATGAEAEAGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNET
                     KTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFI
                     DSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQAMCRG
                     FLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANM
                     KEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADSLADAEERCDQLIK
                     TKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEK
                     HATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIK
                     LEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKE
                     FEMSGLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRE
                     LEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSV
                     AELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSE
                     IKTKEEEQQRLINDLTAQRARLQTESGEYSRQLDEKDTLVSQLSRGKQAFTQQIEELK
                     RQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTK
                     YETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDV
                     ERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKESRSLSTELFKIKNAYEE
                     SLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSELQAALEEAE
                     ASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQMKRNHIRIVESMQSTLDAEIR
                     SRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQAILKDTQLHLDDALRSQ
                     EDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTS
                     LINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLE
                     RMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVK
                     GLRKHERKVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKF
                     RRIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE"
     exon            299..442
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            443..599
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            600..627
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..742
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            743..835
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            836..899
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            900..998
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1102
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1241
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1360
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1510
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1681
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1991
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2062
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2150
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2151..2268
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2392
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2393..2529
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..2785
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..3028
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3029..3205
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3206..3351
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3352..3442
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3443..3832
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3833..3959
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3960..4078
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            4079..4275
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            4276..4459
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            4460..4625
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            4626..4750
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            4751..5059
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            5060..5263
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            5264..5389
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            5390..5560
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            5561..5665
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            5666..5761
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     exon            5762..6023
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6000..6005
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /note="hexamer: GATAAA"
     polyA_site      6023
                     /gene="MYH1"
                     /gene_synonym="HEL71; MYHa; MyHC-2x; MyHC-2X/D; MYHSA1"
                     /note="major polyA site"
ORIGIN      
        1 gtctctcctc ataaagcttc aagttctgat ccactttaag gtcgcatctc tacgccaggg
       61 tccttaactg ggctaccatc aataacctgc agccatgagt tccgactctg agatggccat
      121 ttttggggag gctgctcctt tcctccgaaa gtctgaaagg gagcgaattg aagcccagaa
      181 caagcctttt gatgccaaga catcagtctt tgtggtggac cctaaggagt cctttgtgaa
      241 agcaacagtg cagagcaggg aaggggggaa ggtgacagct aagaccgaag ctggagctac
      301 tgtaacagtg aaagatgacc aagtcttccc catgaaccct cccaaatatg acaagatcga
      361 ggacatggcc atgatgactc atctacacga gcctgctgtg ctgtacaacc tcaaagagcg
      421 ctacgcagcc tggatgatct acacctactc aggcttgttc tgtgtcactg tcaaccccta
      481 caagtggttg ccagtgtata atgcagaggt ggtgacagcc taccgaggca aaaagcgcca
      541 ggaggcccca ccccacatct tctccatctc tgacaatgcc tatcagttca tgctgactga
      601 tcgggagaat cagtctatct tgatcaccgg agaatctggc gcagggaaga ctgtgaacac
      661 caagcgtgtc atccagtact ttgcaacaat tgcagttact ggggagaaga agaaggaaga
      721 agttacttct ggcaaaatgc aggggactct ggaagatcaa atcatcagtg ccaaccccct
      781 actggaggcc tttggcaacg ccaagaccgt gaggaatgac aactcctctc gctttggtaa
      841 attcatcagg atccacttcg gtaccacagg gaaactggct tctgctgata ttgaaacata
      901 tcttctggag aagtctagag ttactttcca gctaaaggct gaaagaagct atcatatttt
      961 ttatcagatc atgtctaaca agaagccaga tctaattgaa atgctcctga tcaccaccaa
     1021 cccatacgat tatgccttcg tcagtcaagg ggagatcaca gtgcccagca ttgatgacca
     1081 agaagagttg atggctacag atagtgccat tgaaattctg ggctttactt cagatgaaag
     1141 agtgtccatc tataagctca caggggctgt gatgcattat gggaacatga aattcaagca
     1201 aaagcagcgt gaggagcaag ctgagccaga tggcactgaa gttgctgaca aggcagccta
     1261 tctccaaaat ctgaactctg cagatctgct caaagccctc tgctacccta gggtcaaggt
     1321 cggcaatgag tatgtcacca aaggtcaaac tgtgcagcag gtgtacaatg cagtgggtgc
     1381 tctggccaaa gctgtctacg ataagatgtt cttgtggatg gtcacccgca tcaaccagca
     1441 gctggacacc aagcagccca ggcagtactt cattggggtc ttggacattg ctggctttga
     1501 gatctttgat ttcaacagcc tggagcagct gtgcatcaac ttcaccaatg agaaactgca
     1561 acagtttttc aaccaccaca tgttcgtgct ggagcaggag gagtacaaga aggaaggcat
     1621 tgagtggacg ttcattgact ttgggatgga cctggctgcc tgcatcgagc tcatcgagaa
     1681 gcctatgggc atcttctcca tcctggaaga ggagtgcatg ttccccaagg cgacagacac
     1741 ctccttcaag aacaagctgt atgaacaaca tcttggaaaa tccaataact tccagaagcc
     1801 caagcctgcc aaaggcaagc ctgaggccca cttctctttg attcactatg ctggcaccgt
     1861 ggactacaac attgccggct ggcttgacaa gaacaaggac cccctgaatg agactgtggt
     1921 ggggctgtac cagaagtctg caatgaagac tctggctctc ctctttgttg gggcaacggg
     1981 agcggaagca gaggctggcg gtggaaagaa aggtggtaag aagaagggtt cttctttcca
     2041 gactgtgtct gctctcttca gggagaattt gaataagctg atgaccaact tgaggagcac
     2101 tcacccccac tttgtgcggt gcatcatccc caatgaaact aaaactcctg gtgccatgga
     2161 gcatgagctt gtcctgcatc agctgaggtg taacggtgtg ctggaaggca tccgcatctg
     2221 caggaaaggc ttcccaagca gaatccttta tgcagacttc aaacagagat acaaggtgtt
     2281 aaatgcaagt gctatccctg aaggacaatt catcgatagc aagaaggctt cagagaagct
     2341 cctggggtcc attgacattg accacaccca gtataaattt ggtcacacca aggtcttttt
     2401 caaagctggt cttctggggc tcctagagga gatgcgagat gagaagctgg cccagctgat
     2461 tacccgaacc caggccatgt gcagagggtt cttggcaaga gtggagtacc agaaaatggt
     2521 ggaaagaaga gagtccatct tctgcatcca gtacaatgtc cgtgccttca tgaatgtgaa
     2581 gcactggccc tggatgaagc tgtatttcaa gatcaaaccc ctcctcaaaa gtgcagagac
     2641 agagaaggag atggccaaca tgaaggaaga atttgagaaa accaaagaag agctggctaa
     2701 gaccgaggca aaaaggaaag agctggaaga aaaaatggtt actctgatgc aagaaaaaaa
     2761 tgacttgcaa ctccaggttc aagctgaagc tgacagcttg gctgatgcag aggaaaggtg
     2821 tgaccagcta atcaaaacca aaatccagct agaagccaaa atcaaagagg tgactgagag
     2881 agctgaggat gaggaagaga tcaatgctga gctgacagcc aagaagagga aactggagga
     2941 tgaatgttca gaactcaaga aagacattga tgaccttgag ctgacactgg ccaaggttga
     3001 gaaggagaaa catgccacag aaaacaaggt gaaaaacctc acagaagaga tggcgggtct
     3061 ggatgaaacc attgctaagc tgaccaagga gaagaaggct ctccaggagg cccaccagca
     3121 gaccctggat gacctgcagg cagaggagga caaagtcaac accctgacca aagctaaaat
     3181 caaacttgaa caacaagtgg atgatcttga aggatctttg gaacaagaaa agaaaatccg
     3241 gatggatcta gaaagagcaa agagaaaact agagggagac ctaaaattgg ctcaagaatc
     3301 cacaatggat atagaaaatg acaaacaaca acttgatgaa aagcttaaaa agaaagagtt
     3361 tgaaatgagc ggtctgcaaa gcaagattga agatgaacaa gcccttggta tgcagctgca
     3421 gaagaaaatc aaggagttac aagcccgcat tgaggagctg gaggaggaaa tcgaggcaga
     3481 gcgggcctcc cgggccaaag cagagaagca gcgctctgat ctctcccggg agctggagga
     3541 gatcagcgag aggctggaag aagccggtgg ggccacctca gcccagattg agatgaacaa
     3601 gaagcgggag gctgagttcc agaaaatgcg cagggacctg gaggaggcca ccctacagca
     3661 tgaagccacg gcggccaccc tgaggaagaa gcatgcagat agtgtggccg agcttgggga
     3721 gcagattgac aacctgcagc gagtgaagca gaagctggag aaggagaaga gtgagatgaa
     3781 gatggagatc gatgaccttg ctagtaacat ggagactgtc tccaaagcca agggaaacct
     3841 tgaaaagatg tgccgcgctc tagaagatca actgagtgaa attaagacca aggaagagga
     3901 gcagcagcgg ctgatcaatg acctcacagc acagagagcg cgcctgcaaa cagaatcagg
     3961 tgaatattca cgccagctag atgaaaagga cacactagtt tcacagctct cgaggggcaa
     4021 acaagccttt acacaacaga ttgaggaact gaaaaggcaa cttgaagagg agataaaggc
     4081 caagagtgcc ctggcacatg ccctgcagtc ctcccgccat gactgtgacc tgctgcggga
     4141 acagtatgag gaggagcagg aagccaaggc cgagctacag agagcaatgt ccaaggccaa
     4201 cagtgaggtt gcccagtgga ggaccaaata tgagacagat gccatccagc gcacagagga
     4261 gctggaggag gccaagaaga agctggctca gcgtctgcag gatgctgagg aacatgtaga
     4321 agctgtgaat gccaaatgtg cttcccttga gaagacgaag cagaggctcc agaatgaagt
     4381 tgaggacctc atgattgatg ttgagaggac aaatgctgcc tgtgccgccc tggacaaaaa
     4441 gcaaaggaac tttgataaga tcctggcaga atggaaacag aagtgtgaag aaactcatgc
     4501 tgaacttgaa gcttctcaaa aggaatcccg ctcactcagc acagaactat ttaagattaa
     4561 gaatgcttat gaggaatctt tagaccaact tgaaaccttg aaacgggaaa ataagaattt
     4621 gcaacaggag atttctgatc tcactgaaca gattgcagaa ggaggaaagc gcatccatga
     4681 actggaaaaa ataaagaagc aagttgagca agaaaagtct gaacttcagg ctgccttaga
     4741 ggaggcagag gcatctcttg aacatgaaga gggaaagatc ctgcgcatcc agcttgagtt
     4801 gaaccaagtc aagtctgagg ttgataggaa aattgctgaa aaagatgagg aaattgacca
     4861 gatgaagaga aaccacatta gaatcgtgga gtccatgcag agcacactgg atgctgagat
     4921 caggagcagg aatgatgcca ttaggctcaa gaagaagatg gagggagacc tcaatgaaat
     4981 ggaaatccag ctgaaccatg ccaaccgcat ggctgctgag gccctgagga actataggaa
     5041 cacccaagcc atcctcaagg atacccagct ccacctggat gatgctctcc ggagccagga
     5101 ggacctgaag gaacagctgg ctatggtgga gcgcagagcc aacctgctgc aggctgagat
     5161 cgaggagctg cgggccactc tggaacagac agagaggagc aggaaaatcg cagaacagga
     5221 gctcctggat gccagtgagc gtgttcagct cctgcacacc cagaacacca gcctgatcaa
     5281 caccaagaag aagctggaga cagacatttc ccaaatccag ggagagatgg aagacatcat
     5341 ccaggaagcc cgcaatgcag aagagaaggc caagaaggcc atcactgatg ctgccatgat
     5401 ggctgaggag ctgaagaagg aacaggacac cagcgcccat ctggagcgga tgaagaagaa
     5461 cctggaacag acggtgaagg acctgcagca tcgtctggat gaggctgagc agctggccct
     5521 gaagggtggg aagaagcaga tccagaaact ggaggccagg gttcgtgaac ttgaaggtga
     5581 agttgaaagt gaacagaagc gcaatgttga agctgtcaag ggtctacgca aacatgagag
     5641 aaaagtgaag gaactcactt accaaactga ggaagaccgc aagaatattc tcaggctcca
     5701 ggacctggtg gacaaactgc aagcaaaggt gaaatcctac aagagacaag ctgaagaagc
     5761 ggaggaacaa tccaacgtca acctctccaa attccgcagg atccagcacg agctggagga
     5821 ggccgaggaa cgggctgaca ttgctgagtc ccaggtcaac aagctgaggg tgaagagcag
     5881 ggaggttcac acaaaaatca taagtgaaga gtaatttatc taactgctga aaggtgacca
     5941 aagaaatgca caaaatgtga aaatctttgt cactccattt tgtacttatg acttttggag
     6001 ataaaaaatt tatctgccaa aaa
//
