LOCUS       NM_016132              10137 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens myelin expression factor 2 (MYEF2), transcript variant
            1, mRNA.
ACCESSION   NM_016132
VERSION     NM_016132.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Zhang,P., Zhao,J.H., Chen,L., Bian,Z.L., Ju,L.L., Wang,H.X. and
            Cai,W.H.
  TITLE     Expression and function of myelin expression factor 2 in
            hepatocellular carcinoma
  JOURNAL   BMC Gastroenterol 23 (1), 20 (2023)
   PUBMED   36658471
  REMARK    GeneRIF: Expression and function of myelin expression factor 2 in
            hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 10137)
  AUTHORS   Malone,S.M., McGue,M. and Iacono,W.G.
  TITLE     What can time-frequency and phase coherence measures tell us about
            the genetic basis of P3 amplitude?
  JOURNAL   Int J Psychophysiol 115, 40-56 (2017)
   PUBMED   27871913
  REMARK    GeneRIF: This study demonstrated that none of the associations
            between time-frequency phenotypes and MYEF2 were significant.
REFERENCE   4  (bases 1 to 10137)
  AUTHORS   Mishra,A., Nizammuddin,S., Mallick,C.B., Singh,S., Prakash,S.,
            Siddiqui,N.A., Rai,N., Carlus,S.J., Sudhakar,D.V.S., Tripathi,V.P.,
            Mols,M., Kim-Howard,X., Dewangan,H., Mishra,A., Reddy,A.G., Roy,B.,
            Pandey,K., Chaubey,G., Das,P., Nath,S.K., Singh,L. and Thangaraj,K.
  TITLE     Genotype-Phenotype Study of the Middle Gangetic Plain in India
            Shows Association of rs2470102 with Skin Pigmentation
  JOURNAL   J Invest Dermatol 137 (3), 670-677 (2017)
   PUBMED   27866970
  REMARK    GeneRIF: both polymorphisms (rs1426654 and rs2470102) play an
            important role in the skin pigmentation diversity of South Asians
REFERENCE   5  (bases 1 to 10137)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   6  (bases 1 to 10137)
  AUTHORS   Lee,K.S., Cao,Y., Witwicka,H.E., Tom,S., Tapscott,S.J. and
            Wang,E.H.
  TITLE     RNA-binding protein Muscleblind-like 3 (MBNL3) disrupts myocyte
            enhancer factor 2 (Mef2) {beta}-exon splicing
  JOURNAL   J Biol Chem 285 (44), 33779-33787 (2010)
   PUBMED   20709755
  REMARK    GeneRIF: RNA-binding protein Muscleblind-like 3 (MBNL3) disrupts
            myocyte enhancer factor 2 (Mef2) {beta}-exon splicing
REFERENCE   7  (bases 1 to 10137)
  AUTHORS   Robertson,N.G., Khetarpal,U., Gutierrez-Espeleta,G.A., Bieber,F.R.
            and Morton,C.C.
  TITLE     Isolation of novel and known genes from a human fetal cochlear cDNA
            library using subtractive hybridization and differential screening
  JOURNAL   Genomics 23 (1), 42-50 (1994)
   PUBMED   7829101
REFERENCE   8  (bases 1 to 10137)
  AUTHORS   McDermott,J.C., Cardoso,M.C., Yu,Y.T., Andres,V., Leifer,D.,
            Krainc,D., Lipton,S.A. and Nadal-Ginard,B.
  TITLE     hMEF2C gene encodes skeletal muscle- and brain-specific
            transcription factors
  JOURNAL   Mol Cell Biol 13 (4), 2564-2577 (1993)
   PUBMED   8455629
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC066612.7, AC090526.3 and KF456039.1.
            
            On Nov 23, 2018 this sequence version replaced NM_016132.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.71665.1,
                                           SRR1660805.190949.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324324.12/ ENSP00000316950.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-219               AC066612.7         97982-98200         c
            220-428             AC066612.7         88536-88744         c
            429-481             AC066612.7         87254-87306         c
            482-489             AC066612.7         86026-86033         c
            490-583             AC066612.7         85838-85931         c
            584-775             AC066612.7         79518-79709         c
            776-929             AC066612.7         78674-78827         c
            930-979             AC066612.7         78080-78129         c
            980-1043            AC066612.7         77898-77961         c
            1044-1145           AC090526.3         166216-166317       c
            1146-1196           AC090526.3         164658-164708       c
            1197-1265           AC090526.3         164298-164366       c
            1266-1364           KF456039.1         166-264             c
            1365-1436           AC090526.3         163524-163595       c
            1437-1645           AC090526.3         161587-161795       c
            1646-1697           AC090526.3         161456-161507       c
            1698-10137          AC090526.3         147056-155495       c
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.1"
     gene            1..10137
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /note="myelin expression factor 2"
                     /db_xref="GeneID:50804"
                     /db_xref="HGNC:HGNC:17940"
                     /db_xref="MIM:619395"
     exon            1..219
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..1861
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /note="isoform a is encoded by transcript variant 1;
                     myelin gene expression factor 2"
                     /codon_start=1
                     /product="myelin expression factor 2 isoform a"
                     /protein_id="NP_057216.3"
                     /db_xref="CCDS:CCDS32230.1"
                     /db_xref="GeneID:50804"
                     /db_xref="HGNC:HGNC:17940"
                     /db_xref="MIM:619395"
                     /translation="MADANKAEVPGATGGDSPHLQPAEPPGEPRREPHPAEAEKQQPQ
                     HSSSSNGVKMENDESAKEEKSDLKEKSTGSKKANRFHPYSKDKNSGAGEKKGPNRNRV
                     FISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALET
                     MNKYDLSGRPLNIKEDPDGENARRALQRTGGSFPGGHVPDMGSGLMNLPPSILNNPNI
                     PPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMG
                     TVTFEQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVPHEEYRSHDGKTPQLPRGLGGI
                     GMGLGPGGQPISASQLNIGGVMGNLGPGGMGMDGPGFGGMNRIGGGIGFGGLEAMNSM
                     GGFGGVGRMGELYRGAMTSSMERDFGRGDIGINRGFGDSFGRLGSAMIGGFAGRIGSS
                     NMGPVGSGISGGMGSMNSVTGGMGMGLDRMSSSFDRMGPGIGAILERSIDMDRGFLSG
                     PMGSGMRERIGSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMENGKSKGCGT
                     VRFDSPESAEKACRIMNGIKISGREIDVRLDRNA"
     exon            220..428
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            429..481
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            482..489
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            490..583
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            584..775
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            776..929
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            930..979
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1043
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1145
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1196
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1265
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1364
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1436
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1645
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1697
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..10137
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2949..2954
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /note="hexamer: ATTAAA"
     polyA_site      2972
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /note="major polyA site"
     regulatory      5317..5322
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /note="hexamer: AATAAA"
     polyA_site      5342
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
     regulatory      10118..10123
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
                     /note="hexamer: AATAAA"
     polyA_site      10137
                     /gene="MYEF2"
                     /gene_synonym="HsT18564; MEF-2; MST156; MSTP156; myEF-2"
ORIGIN      
        1 attgtcgcgc tcggagcccc tcagctcagg cggccgaggc ggaggcagcg gcggcgggat
       61 ggcggacgcc aacaaggccg aggtgcccgg ggccactggt ggcgacagcc cgcacctgca
      121 gcccgcagag ccgccgggcg agccgcggcg agagccgcac cccgcggagg cggagaagca
      181 gcagccgcag cacagcagca gctccaatgg cgttaaaatg gagaatgatg aatcagcaaa
      241 agaagagaaa tctgacttaa aggaaaaatc tacaggaagt aagaaggcca atagatttca
      301 tccttattca aaagacaaga attcgggcgc tggagaaaag aagggtccaa atcgtaacag
      361 agttttcatt agcaacatcc catatgacat gaaatggcaa gctattaaag atctaatgag
      421 agagaaagtt ggtgaggtta catacgtgga gctctttaag gatgcggaag gaaaatcaag
      481 gggttgtggt gtggttgaat tcaaagatga agaatttgta aagaaagccc tagaaactat
      541 gaacaaatat gatcttagtg gaagacccct taatattaaa gaggatcctg atggagaaaa
      601 tgctcgtagg gcattgcagc gaacaggagg atcatttcca ggaggacacg tccctgatat
      661 gggatcaggg ttgatgaatt taccaccttc catactcaat aatccaaaca ttcctcctga
      721 agtcatcagt aatttgcagg ccggtagact tggttccaca atttttgttg ccaatcttga
      781 cttcaaagtt ggttggaaga agctaaagga agtgttcagc atagctggaa ctgtgaagcg
      841 ggcagatatt aaagaagaca aagatggcaa gagcagagga atgggcactg tcacttttga
      901 gcaagcaatt gaagcagttc aagcaatttc tatgttcaat gggcagtttt tatttgatag
      961 acctatgcat gtgaaaatgg atgacaagtc tgttcctcat gaagagtacc gttcacatga
     1021 tggtaaaaca ccacaattac cacgtggtct tggaggcatt gggatgggac ttggtccggg
     1081 tggacagcct attagtgcca gccagttgaa cataggtgga gtaatgggaa atttaggtcc
     1141 aggtggtatg ggaatggatg gtccaggttt tggaggaatg aatagaattg gaggaggaat
     1201 agggtttggt ggtctggaag caatgaatag catgggagga tttggaggag ttggccgaat
     1261 gggagagctg taccgtggtg cgatgactag tagcatggag cgagattttg gacgtggtga
     1321 tattggaata aatcgaggct ttggagattc ctttggtaga cttggcagtg caatgattgg
     1381 agggtttgca ggaagaatag gatcttctaa catgggtcca gtaggatctg gaataagtgg
     1441 tggaatgggt agcatgaaca gtgtgactgg aggaatgggg atgggactgg accggatgag
     1501 ttccagcttt gatagaatgg gaccaggtat aggagctata ctggaaagga gcatcgatat
     1561 ggatcgagga tttttatcgg gtccaatggg aagcggaatg agagagagaa taggctccaa
     1621 aggcaaccag atatttgtca gaaatctacc ttttgacttg acttggcaga aactaaaaga
     1681 gaaattcagt cagtgtggtc atgtaatgtt tgcagaaata aaaatggaga atggaaagtc
     1741 aaaaggctgt ggaacagtca gatttgactc cccagaatca gctgaaaaag cctgcagaat
     1801 aatgaatggc ataaaaatca gtggcagaga aattgatgtt cgcttggatc gtaatgcata
     1861 atttcaagcc atggttggaa cattcctaca tctgttttgc tgaatctcct agtaaaagtc
     1921 atttttttaa agtaatattg tatgcttaca aaagctgtaa aaatgaactt ttaaaactcc
     1981 caccagcttt taacagtata atgttaaaaa tatactgtaa tttttgttaa tctcaagttt
     2041 gggtttttaa agacagcaag tctggtcatt cagtttaaat gaatggttat actgttttta
     2101 atgaaataag ccattttctt gttgttttca gtactacata gttggatttg tttgttctag
     2161 tttctccagt ttttgtcaac ttattgtatg gtaaaacata gattttttcc ccccaaactt
     2221 ctgttttata atatgtaatt tttcatgaaa agaaagggct cagaaaaatt aggatgtgat
     2281 tttggttggt tttaaattac tgtaagtttt agattctaag gttcaagatt tttaaaattt
     2341 gatttaaatg aagaaatgga tttttctctc tgcccctccc tgccattcat attttctgca
     2401 taacactatt aataatatca acctccacag ccccttattt tattatttcc aataattcca
     2461 agttcatata gaactgataa tgtagcaagc cccaagtatg ataataggca gactattccc
     2521 aactttctgt ctagtttcca gccattgaag tgaactgcta aaaaaagaaa aataattgaa
     2581 atgttgagag agatggttat gtaagttagt cctctgctgt ttacttctgc aggagctgat
     2641 ccatttataa atgcagtttt aataaaccat ggaataccaa ggcacaacat atcaaacaca
     2701 ttggatccca cgatgttaga catagccata tctcctttcc ctacaaaaga aaagcatact
     2761 gttaaaatgt gcttaccaat actgtgtttt attaataatc ttcataagaa aagaaacact
     2821 ggatactttt ttgttgttga ttcgttttga aaaattgttg ttagagcaaa agtcttactg
     2881 aatttgtatt tttaaatttt tcttgggtta gtatttaaag tcttaacatt tatttaataa
     2941 ttatatttat taaaccattt ttcaataagg tatatagctt atttgttgct tttcattgta
     3001 atttaacatg gttaatggtt aattactatt taacacacat ttcaaatgaa tattatttgg
     3061 gggattagat tgagtgaaat taacctgcta ttaaatagta aacttttcct ctggagtcac
     3121 ttttttcccc cttcaaagta tgttactgag gaagtaaact tttttttttt tttttggttt
     3181 ttgttttttg agacacagtc tcgctctgtt gcccaggctg ctggagtgcc gtggcgcaat
     3241 ctcggctcac tgcaacctcc gcctcctgga ttcaaacaat tctcctgcct cagcctcctg
     3301 agtagctggg attacaggca catgccacca cgcccggcta atttttgtat ttttagtaga
     3361 gactgggttt caccatgttg gtcaggctgg tctcaaactc ctgacctcgt gatccacccg
     3421 cctcggcctc ccaaaatcct gggattacag gcgcgagcca ccacacccgg ctggaagtaa
     3481 acatttttaa agctactttt actcattcta gccttgtaga atgaccattg cagcttgagg
     3541 gacctagttc ttaccttttc ttgcaaccaa cacacttgca attgtgtctg gtatgcttgt
     3601 tcctgctgct aataaagtaa ggcccattac tgtatcggga atttctagtg tttcccctgt
     3661 aataaacaga tatttcaagt tacaaatctt aaagattcac taaccatcct ttgcagttat
     3721 tttggatatt tccttcgtga acaaaaataa aataggcaca tttagaattc agagccaata
     3781 tgtgcttgct tattagtttt ttagctagca acatatttga atcaggctgg taattcgggt
     3841 aacccaggta gcacagattt ttaatgacat atctaaagat acgtaacagc taaaattctg
     3901 ccagtgagaa attttcctgt ttgatattct tacaaaagat gtttatgtcc accattatct
     3961 catcagggct gtgctgaata tttgataatg agactgatca ttccgctttt tctttcttaa
     4021 aaatattagt cagagttaag caaattaatt atagctatct ttaagctata aatgtgttaa
     4081 catgtatata taccatttat tatgttctac tttagtgata taccttaatt tagtgggctt
     4141 tggcagggcg ggggaggggg aacgttcatt aatctctgag gaaaacaaaa cctgttttct
     4201 acttgagtct aacatatggt cccaatttat taatacttct gttaaatttg atgtcaggtc
     4261 aacatttttc agaaatgtat ttattctcag aaacagaacc agagagaagt taaacaaaag
     4321 gttatgtaac tgttccttta atgttgtaat tgaaaacttg gtttagcgtc ttttttttct
     4381 ttctcttttt ttttcttaaa atgccaacta aaataattag aaagtagctt atttattgca
     4441 tgcttataca ttgatattgg aattggaatt ggttgttaat ttctgttact ggctttgcta
     4501 gaattcatat gtgcataaat aacactaata tttatcatct tgggggctgt tctgtgatct
     4561 attacatgat ttttgctctc tttttatttt tctagtatca aatgtgttct ggctgagtga
     4621 atttggatag atataatatg caagctaatt ggactttttc atttttctca ttaaaaaaag
     4681 gaagaagtag tccacttttt gctagcatac tattctgaga cttacattaa tttgggaaaa
     4741 gtcaagggtc aacaaatttt tgtgtctgtg aggggccaga tagtaattat ttaggctttg
     4801 caggctgtgt gatttctgtt gcagttattc agctttgcca atgtagtgtg aaaacaatca
     4861 tagataatat gtaaaacctt actaataaaa acaagctggg ttatgttggc acacaggcag
     4921 tagtttgttc acctctgctg taagttaatt tgggttaacc tttgtaggag aaattacaga
     4981 ttatttttgt ctttgttcat agtttttttg tttagggact gtagtggatt atggccaagc
     5041 agcagtagct agttaagtct taatcatgaa ggtagtagaa acctagaata aaattattat
     5101 gcccttttca ctcatttcat aatatgtttg tattgccgtg ttgaaaagcc aaataaaata
     5161 caatccagtt aagcagtgag cacccattgt tttcccaaaa atagagctcc ttgctttgtt
     5221 tttgtttttt tgttcttttt tttttttatt atggaatgga attcaatata aagagtttat
     5281 tgtggacccc atggaatgtg attaaattat tagcataata aaaagacatc ctatgaattt
     5341 gatttcattt ataagatctc ttgaattgta ccccaggcta aataacagtg gaaaatacag
     5401 tttatatata atatgcaact cacaaactcc agtgatcttg ttatagtaat acttaatcat
     5461 tttgtaaatg gtactaatct tcaatcttgc tgaaaagaaa tttgctgtgt agctagacta
     5521 ctttgagtga acatatgcaa tttgttctta tatgaatatt ttcaagttgt gctattgtac
     5581 ttaaaataca taccagttat tgtgaccatc caaaccagga tatatgtaaa tgcggatatc
     5641 catattgcag acatgaaaaa ggttatcaca aagtagtttt tccaaaactt ttttctacaa
     5701 tctggtgtgg ttagaaaaag taatgtaata ataggaaggg ataataccca aaaaattctt
     5761 tttaagtctg cttcaggcat gttgaaaaca cttggtggat ctgttgtgga aaaagtaaac
     5821 acttttgagt tttctcaaat ggctaattaa gttagaaatg cctatttaga aagttatatt
     5881 gaaataaatt agctgtatca gtttttaaaa ggctgtttaa aacatttgag tacctcatta
     5941 ctgatattgc tggtgaaaag aaactaattg aaactttagg gcagacaatg taagatgtga
     6001 tactccgcat ttctttattt ggggccttaa atctataaaa atgtcttggg ccattcgtta
     6061 aatatattgt agatagtgtt tatgaagtgt caattggaca tctcctgtgg attgtcttca
     6121 gtaacttttt gcttaaggga ttaacctggt ttctaaccta gctactccag ttgtctccac
     6181 ttcataatat tgatgatgcc tctgcctgcc ctatgtacca gataatgtat acttaaagaa
     6241 tataaaataa tcatagcata atttttttat taagtcgcat atcctaattc ttagtggaag
     6301 tggttttttt gtcctatttc attactattt tatttattaa attcagactt taccacagtt
     6361 catagaaata accttaaagt aggaggtagt atatcatcta aagtacaatt tatgaaaatt
     6421 gagtatattt ctttattcaa tttaatttag tagaaggata atgataatat attggataaa
     6481 aattcgaatt caaaaatgtt caaaatttgt atggcattta caaaataatg taacaattgg
     6541 atgaaatcat gttaaaacta atgttagtac aacaactcat aaaaagtttg ccaagcttca
     6601 accaatgtgc cattaagtaa tattagatat aattttaaca taatcaaaga atttggattg
     6661 gttcttttgc gtttcttatt ataaacttaa tttggagagg catacctctt atacctgaaa
     6721 gagtagaagc taaaattttt gtaaaaaggg ggaaaagtca tgttttaaaa ttttaagtag
     6781 tagattagaa taaaaccctc ctaaaggctt aattaaattt agaatctgtt ttggacaacc
     6841 actttataaa aaggtaaccc atgaacttat aattttgacc atagctgaat tttctgactt
     6901 cctactgaac ctatttacct aaaagttttg ctattgcttt aaacaatggt aaacctcagc
     6961 tgtttttctt ctttcttcca aaccagcccc tttttctgag ttcccagttt taataagtgc
     7021 tgttcccatt ttctcagtca agattaaaac ataggaggtg tctttgttcc tttttctggc
     7081 cccatatagt cttggtgatg cttccttcat atagtctcac atctgttctc atttattccc
     7141 accactttca cttggtctag gcacctagta aacacatcag ctgttgtaat agcctctctt
     7201 acagacctct ttgtctcttc atattttagt tcggtgaaca tcattttggg taacattact
     7261 aagataacag ttctaataat ttctctcctc agaaaccttc caagattctt aaaatagcat
     7321 ttaagattct tcttatccta ttcctttcta cctttcctct ccttccttcc tatgcacata
     7381 ctgtgctcta tgtgttttat ctctgcatca tttatggaat gaagacctca gtagctgcta
     7441 cttattggtc ataacatatg tcattagtta aagtctttcc tgtccagcaa ggcccaattt
     7501 aaatgctccc ttctctgtga aaatctccta tctgtcctca ccacattttg gcaatctgtc
     7561 ccatttgtga accacagtca cactttattt ttctcttttt atggtactta acgttcatac
     7621 tttagctatt ttgtatttcc tgtgatattg gaagttcctt aaaggtaggg acttggtctt
     7681 tatcatttct gagtcttcac agtctttttt acataaaaag cacacaatac ttgctgaatt
     7741 gaaatgaaat tttaaagttt ttcctttgcg aatagactta ataatgggca agatttagtg
     7801 attaccttca gaaacctgac taaggccatg taaacttata gagagctgag agtagccaga
     7861 atcttcataa aatattccac tatcagttct tgattgccga cgaatgaatg gttgaccttc
     7921 atcttcccag cccatcagtg gctgttgttc acttctctcc atagctttgg caagacaggc
     7981 gcagcaagga ctgcatttct ttataatata ttggttaatt ttaatgtcaa aacacagcac
     8041 caaaacatac aatccatata tcaaaagcag taaagcccct tcatacctac aaaagagaaa
     8101 tttgtgtttt taaattaaaa gtgaaagtca ttttagagtt gatagctttt gggcagtatc
     8161 tattgaatga ttttctaaca aaagtagcca tagaacaatc aaacatttct agatattttt
     8221 tgttttgtag atttgtccag acttgattac agcattatta tgtttattga taaaagctgg
     8281 acacttctgt gttgtttttt tttttttaaa aacaagatca acaaactcgt ttttacttgt
     8341 agattccttc caaatgatag gtataaaaat gcaaggaaaa tgataattgg agattttcaa
     8401 aataaatgta aaattaataa atagtggtga acattttctg cctcagctca ttggcataat
     8461 acattcccca acatacataa taattataaa ataatatagt aactgttaag ttatttcaac
     8521 tgtccaagtt ttctggtata ggaactttgt gtaaaatatt actcagatat gggaggctta
     8581 ggctgggatt tttcagtttg tcatacagag catttttaat gatttgaatg tagctgaatt
     8641 tggcttacag tcacaagaaa acaagatgtt ctaaaatata aaattatacc cttggaacca
     8701 agaactgttt ccctgtactc tggcagttag ctaatgccat cacatttgaa ccttgcattt
     8761 accttttata gccagtgaat ctctgtctgc tgtatcttaa agaacagttc tgatttccct
     8821 aatggaggaa gaattagctt ggatactctc acagtcacta tcccttacag acaactgctg
     8881 agttctagaa ggatttctat tctaccaaca cagtgtctta gggagacgga ctagaggaca
     8941 ggatgattag tttagaaatt atgcttctgg ctaggtttta agtttcttga tagcaggtat
     9001 tacgccgtgc tgcaaggtta tggttctcca gatggacacc attacatttt ttttttttca
     9061 tatttttctg gtgtcctctg gagttatgca atgtatataa cctgtgtagc tgtgttttac
     9121 atttcattat cccttatgat atctagcata gtgagaagga cctagtaggt gcctgctgct
     9181 tactagttga aaaagttagt atctgtccac tcagtcacca cctttttcat tttcttggac
     9241 cagctaggca cagagagatt gtaaggaaag caaacaattt ttaatatgaa aagctaagca
     9301 ttgttatttc ttagaggaag gctatagttt ctcctgaatg agcatcagaa gtttggagga
     9361 atttaaaaag acttctttta cttcctctac catccttctc cagtcttttg tggccatgaa
     9421 cacttctaac caaggcaagg tgagcagtaa cccagcagaa gaaaaagtca actgttgtgg
     9481 ctttaatgga caacaggagt tgggaaactt tctgaatagg cagccagtgg ggagaaggaa
     9541 aagtggtggt ctccagtgac tagtgagaga aaaaaggaga ctgtgaagtg attaagaaga
     9601 aattgcaaac tagtgagaag aactcaatca gcaattgtga tgaggttaac tcacagacct
     9661 gttggaatat ggtatagaag tggcagatta agtgactgaa ataacatttc tgaaaaaaaa
     9721 ttactgaacc atcagaaatt cataaaatct ctaattttta cataagaatt attttcaagc
     9781 ttaccagtaa acttggttgt catatattat accaagaact gctgctgcac taattgtgta
     9841 cgctgcacag tctctgaata ggggccaaca tgatagtgtt gagacctgta atatggtaag
     9901 ttattatttc cattacattg taacttcttc aagtacaatc cttagtataa tactaaaatt
     9961 gtttatatat ttgtagttaa atattgttat cttttgcata ttgcttagag aaaaaaatgt
    10021 aaaattgtgt tctaacttac acagactttc ataaatctga ccaagagcta tttgtggatt
    10081 aacattaaat tgaatacatg ttgtttttct actgattaat aaatttgatt gcacata
//
