LOCUS       NM_001282582            2310 bp    mRNA    linear   PRI 13-FEB-2025
DEFINITION  Homo sapiens matrix remodeling associated 8 (MXRA8), transcript
            variant 5, mRNA.
ACCESSION   NM_001282582
VERSION     NM_001282582.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Miao,M., Song,Y., Jin,M., Du,Y., Xin,P., Jiang,Y. and Zhang,H.
  TITLE     Single-cell RNA combined with bulk RNA analysis to explore
            oxidative stress and energy metabolism factors and found a new
            prostate cancer oncogene MXRA8
  JOURNAL   Aging (Albany NY) 16 (5), 4469-4502 (2024)
   PUBMED   38441550
  REMARK    GeneRIF: Single-cell RNA combined with bulk RNA analysis to explore
            oxidative stress and energy metabolism factors and found a new
            prostate cancer oncogene MXRA8.
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Simpson,K.E., Staikos,C.A., Watson,K.L. and Moorehead,R.A.
  TITLE     Loss of MXRA8 Delays Mammary Tumor Development and Impairs
            Metastasis
  JOURNAL   Int J Mol Sci 24 (18), 13730 (2023)
   PUBMED   37762032
  REMARK    GeneRIF: Loss of MXRA8 Delays Mammary Tumor Development and Impairs
            Metastasis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2310)
  AUTHORS   Simpson,K.E., Watson,K.L. and Moorehead,R.A.
  TITLE     Elevated Expression of miR-200c/141 in MDA-MB-231 Cells Suppresses
            MXRA8 Levels and Impairs Breast Cancer Growth and Metastasis In
            Vivo
  JOURNAL   Genes (Basel) 13 (4), 691 (2022)
   PUBMED   35456497
  REMARK    GeneRIF: Elevated Expression of miR-200c/141 in MDA-MB-231 Cells
            Suppresses MXRA8 Levels and Impairs Breast Cancer Growth and
            Metastasis In Vivo.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2310)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2310)
  AUTHORS   Song,H., Zhao,Z., Chai,Y., Jin,X., Li,C., Yuan,F., Liu,S., Gao,Z.,
            Wang,H., Song,J., Vazquez,L., Zhang,Y., Tan,S., Morel,C.M., Yan,J.,
            Shi,Y., Qi,J., Gao,F. and Gao,G.F.
  TITLE     Molecular Basis of Arthritogenic Alphavirus Receptor MXRA8 Binding
            to Chikungunya Virus Envelope Protein
  JOURNAL   Cell 177 (7), 1714-1724 (2019)
   PUBMED   31080063
  REMARK    GeneRIF: the stalk region of MXRA8 is critical for CHIKV virus
            entry.
REFERENCE   6  (bases 1 to 2310)
  AUTHORS   Basore,K., Kim,A.S., Nelson,C.A., Zhang,R., Smith,B.K., Uranga,C.,
            Vang,L., Cheng,M., Gross,M.L., Smith,J., Diamond,M.S. and
            Fremont,D.H.
  TITLE     Cryo-EM Structure of Chikungunya Virus in Complex with the Mxra8
            Receptor
  JOURNAL   Cell 177 (7), 1725-1737 (2019)
   PUBMED   31080061
  REMARK    GeneRIF: studies provide insight into how Mxra8 binds CHIKV and
            creates a path for developing alphavirus entry inhibitors.
REFERENCE   7  (bases 1 to 2310)
  AUTHORS   Han,S.W., Jung,Y.K., Lee,E.J., Park,H.R., Kim,G.W., Jeong,J.H.,
            Han,M.S. and Choi,J.Y.
  TITLE     DICAM inhibits angiogenesis via suppression of AKT and p38 MAP
            kinase signalling
  JOURNAL   Cardiovasc Res 98 (1), 73-82 (2013)
   PUBMED   23386276
  REMARK    GeneRIF: exogenous expression of DICAM in human umbilical vein
            endothelial cells suppressed angiogenesis in vitro Matrigel and in
            vivo plug assays, and conversely, DICAM knockdown enhanced
            angiogenesis
REFERENCE   8  (bases 1 to 2310)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (bases 1 to 2310)
  AUTHORS   Yonezawa,T., Ohtsuka,A., Yoshitaka,T., Hirano,S., Nomoto,H.,
            Yamamoto,K. and Ninomiya,Y.
  TITLE     Limitrin, a novel immunoglobulin superfamily protein localized to
            glia limitans formed by astrocyte endfeet
  JOURNAL   Glia 44 (3), 190-204 (2003)
   PUBMED   14603461
  REMARK    GeneRIF: new member of the transmembrane-type immunoglobulin
            superfamily and designated limitrin, localizing selectively to glia
            limitans in mouse brain
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL139287.24.
            
            On Aug 14, 2020 this sequence version replaced NM_001282582.1.
            
            Transcript Variant: This variant (5) differs in both UTRs and uses
            an alternate splice site in the 3' terminal exon compared to
            variant 1. The encoded isoform (2) is shorter and has a distinct
            C-terminus compared to isoform 1. Variants 2 and 5 encode the same
            isoform (2).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB052096.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AL139287.24        63470-63494         c
            26-117              AL139287.24        58409-58500         c
            118-141             AL139287.24        56634-56657         c
            142-444             AL139287.24        55403-55705         c
            445-546             AL139287.24        55197-55298         c
            547-1017            AL139287.24        54635-55105         c
            1018-1173           AL139287.24        54307-54462         c
            1174-1213           AL139287.24        54146-54185         c
            1214-1290           AL139287.24        53983-54059         c
            1291-1371           AL139287.24        53801-53881         c
            1372-2310           AL139287.24        52644-53582         c
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.33"
     gene            1..2310
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /note="matrix remodeling associated 8"
                     /db_xref="GeneID:54587"
                     /db_xref="HGNC:HGNC:7542"
                     /db_xref="MIM:617293"
     exon            1..25
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            26..117
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..1397
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 5; limitrin; matrix-remodeling-associated protein
                     8; matrix-remodelling associated 8"
                     /codon_start=1
                     /product="matrix remodeling-associated protein 8 isoform 2
                     precursor"
                     /protein_id="NP_001269511.1"
                     /db_xref="CCDS:CCDS24.1"
                     /db_xref="GeneID:54587"
                     /db_xref="HGNC:HGNC:7542"
                     /db_xref="MIM:617293"
                     /translation="MALPSRILLWKLVLLQSSAVLLHSGSSVPAAAGSSVVSESAVSW
                     EAGARAVLRCQSPRMVWTQDRLHDRQRVLHWDLRGPGGGPARRLLDLYSAGEQRVYEA
                     RDRGRLELSASAFDDGNFSLLIRAVEETDAGLYTCNLHHHYCHLYESLAVRLEVTDGP
                     PATPAYWDGEKEVLAVARGAPALLTCVNRGHVWTDRHVEEAQQVVHWDRQPPGVPHDR
                     ADRLLDLYASGERRAYGPLFLRDRVAVGADAFERGDFSLRIEPLEVADEGTYSCHLHH
                     HYCGLHERRVFHLTVAEPHAEPPPRGSPGNGSSHSGAPGPDPTLARGHNVINVIVPES
                     RAHFFQQLGYVLATLLLFILLLVTVLLAARRRRGGYEYSDQKSGKSKGKDVNLAEFAV
                     AAGDQMLYRSEDIQLDYKNNILKERAELAHSPLPAKYIDLDKGFRKENCK"
     exon            118..141
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            142..444
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            445..546
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            547..1017
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1173
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1213
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1290
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1371
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..2310
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2287..2292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /note="hexamer: AATAAA"
     polyA_site      2310
                     /gene="MXRA8"
                     /gene_synonym="ASP3"
                     /note="major polyA site"
ORIGIN      
        1 aatagcttga aactggaagg cagagactta gagccgagtg ggacaaagcc tggggctggg
       61 cgggggccat ggcgctgcca tcccgaatcc tgctttggaa acttgtgctt ctgcagagct
      121 ctgctgttct cctgcactca gggtcctcgg tacccgccgc tgctggcagc tccgtggtgt
      181 ccgagtccgc ggtgagctgg gaggcgggcg cccgggcggt gctgcgctgc cagagcccgc
      241 gcatggtgtg gacccaggac cggctgcacg accgccagcg cgtgctccac tgggacctgc
      301 gcggccccgg gggtggcccc gcgcggcgcc tgctggactt gtactcggcg ggcgagcagc
      361 gcgtgtacga ggcgcgggac cgcggccgcc tggagctctc ggcctcggcc ttcgacgacg
      421 gcaacttctc gctgctcatc cgcgcggtgg aggagacgga cgcggggctg tacacctgca
      481 acctgcacca tcactactgc cacctctacg agagcctggc cgtccgcctg gaggtcaccg
      541 acggcccccc ggccaccccc gcctactggg acggcgagaa ggaggtgctg gcggtggcgc
      601 gcggcgcacc cgcgcttctg acctgcgtga accgcgggca cgtgtggacc gaccggcacg
      661 tggaggaggc tcaacaggtg gtgcactggg accggcagcc gcccggggtc ccgcacgacc
      721 gcgcggaccg cctgctggac ctctacgcgt cgggcgagcg ccgcgcctac gggccccttt
      781 ttctgcgcga ccgcgtggct gtgggcgcgg atgcctttga gcgcggtgac ttctcactgc
      841 gtatcgagcc gctggaggtc gccgacgagg gcacctactc ctgccacctg caccaccatt
      901 actgtggcct gcacgaacgc cgcgtcttcc acctgacggt cgccgaaccc cacgcggagc
      961 cgcccccccg gggctctccg ggcaacggct ccagccacag cggcgcccca ggcccagacc
     1021 ccacactggc gcgcggccac aacgtcatca atgtcatcgt ccccgagagc cgagcccact
     1081 tcttccagca gctgggctac gtgctggcca cgctgctgct cttcatcctg ctactggtca
     1141 ctgtcctcct ggccgcccgc aggcgccgcg gaggctacga atactcggac cagaagtcgg
     1201 gaaagtcaaa ggggaaggat gttaacttgg cggagttcgc tgtggctgca ggggaccaga
     1261 tgctttacag gagtgaggac atccagctag attacaaaaa caacatcctg aaggagaggg
     1321 cggagctggc ccacagcccc ctgcctgcca agtacatcga cctagacaaa gggttccgga
     1381 aggagaactg caaataggga ggccctgggc tcctggctgg gccagcagct gcacctctcc
     1441 tgtctgtgct cctcggggca tctcctgatg ctccggggct cacccccctt ccagcggctg
     1501 gtcccgcttt cctggaattt ggcctgggcg tatgcagagg ccgcctccac acccctctcc
     1561 caggggcttg gtggcagcat agcccccacc cctgcggcct ttgctcacgg gtggccctgc
     1621 ccacccctgg cacaaccaaa atcccactga tgcccatcat gccctcagac ccttctgggc
     1681 tctgcccgct gggggcctga agacattcct ggaggacact cccatcagaa cctggcagcc
     1741 ccaaaactgg ggtcagcctc agggcaggag tcccactcct ccagggctct gctcgtcggg
     1801 ggctgggaga tgttcctgga ggaggacact cccatcagaa cttggcagcc ttgaagttgg
     1861 ggtcagcctc ggcaggagtc ccactcctcc tggggtgctg cctgccaccg agagctcccc
     1921 cacctgtacc accatgtggg actccaggca ccatctgttc tccccaggga cctgctgact
     1981 tgaatgccag cccttgctcc tctgtgttgc tttgggccac ctggggctgc accccttgcc
     2041 ctttctctgc cccatcccta ccctagcctt gctctcagcc accttgatag tcactgggct
     2101 ccctgtgact tctgaccctg acacccctcc cttggactct gcctgggctg gagtctaggg
     2161 ctggggctac atttggcttc tgtactggct gaggacaggg gagggagtga agttggtttg
     2221 gggtggcctg tgttgccact ctcagcaccc cacatttgca tctgctggtg gacctgccac
     2281 catcacaata aagtccccat ctgattttta
//
