LOCUS       NM_005592               8294 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens muscle associated receptor tyrosine kinase (MUSK),
            transcript variant 1, mRNA.
ACCESSION   NM_005592
VERSION     NM_005592.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Uyen Dao,T.M., Barbeau,S., Messeant,J., Della-Gaspera,B.,
            Bouceba,T., Semprez,F., Legay,C. and Dobbertin,A.
  TITLE     The collagen ColQ binds to LRP4 and regulates the activation of the
            Muscle-Specific Kinase-LRP4 receptor complex by agrin at the
            neuromuscular junction
  JOURNAL   J Biol Chem 299 (8), 104962 (2023)
   PUBMED   37356721
  REMARK    GeneRIF: The collagen ColQ binds to LRP4 and regulates the
            activation of the Muscle-Specific Kinase-LRP4 receptor complex by
            agrin at the neuromuscular junction.
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Zhang,S., Ohkawara,B., Ito,M., Huang,Z., Zhao,F., Nakata,T.,
            Takeuchi,T., Sakurai,H., Komaki,H., Kamon,M., Araki,T. and Ohno,K.
  TITLE     A mutation in DOK7 in congenital myasthenic syndrome forms
            aggresome in cultured cells, and reduces DOK7 expression and MuSK
            phosphorylation in patient-derived iPS cells
  JOURNAL   Hum Mol Genet 32 (9), 1511-1523 (2023)
   PUBMED   36579833
  REMARK    GeneRIF: A mutation in DOK7 in congenital myasthenic syndrome forms
            aggresome in cultured cells, and reduces DOK7 expression and MuSK
            phosphorylation in patient-derived iPS cells.
REFERENCE   3  (bases 1 to 8294)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 8294)
  AUTHORS   Mori,S., Suzuki,S., Konishi,T., Kawaguchi,N., Kishi,M.,
            Kuwabara,S., Ishizuchi,K., Zhou,H., Shibasaki,F., Tsumoto,H.,
            Omura,T., Miura,Y., Mori,S., Higashihara,M., Murayama,S. and
            Shigemoto,K.
  TITLE     Proteolytic ectodomain shedding of muscle-specific tyrosine kinase
            in myasthenia gravis
  JOURNAL   Exp Neurol 361, 114300 (2023)
   PUBMED   36525997
  REMARK    GeneRIF: Proteolytic ectodomain shedding of muscle-specific
            tyrosine kinase in myasthenia gravis.
REFERENCE   5  (bases 1 to 8294)
  AUTHORS   Chevessier,F., Faraut,B., Ravel-Chapuis,A., Richard,P., Gaudon,K.,
            Bauche,S., Prioleau,C., Herbst,R., Goillot,E., Ioos,C.,
            Azulay,J.P., Attarian,S., Leroy,J.P., Fournier,E., Legay,C.,
            Schaeffer,L., Koenig,J., Fardeau,M., Eymard,B., Pouget,J. and
            Hantai,D.
  TITLE     MUSK, a new target for mutations causing congenital myasthenic
            syndrome
  JOURNAL   Hum Mol Genet 13 (24), 3229-3240 (2004)
   PUBMED   15496425
  REMARK    GeneRIF: Missense mutation does not affect MuSK catalytic kinase
            activity but diminishes expression and stability.
REFERENCE   6  (bases 1 to 8294)
  AUTHORS   Apel,E.D., Glass,D.J., Moscoso,L.M., Yancopoulos,G.D. and
            Sanes,J.R.
  TITLE     Rapsyn is required for MuSK signaling and recruits synaptic
            components to a MuSK-containing scaffold
  JOURNAL   Neuron 18 (4), 623-635 (1997)
   PUBMED   9136771
REFERENCE   7  (bases 1 to 8294)
  AUTHORS   DeChiara,T.M., Bowen,D.C., Valenzuela,D.M., Simmons,M.V.,
            Poueymirou,W.T., Thomas,S., Kinetz,E., Compton,D.L., Rojas,E.,
            Park,J.S., Smith,C., DiStefano,P.S., Glass,D.J., Burden,S.J. and
            Yancopoulos,G.D.
  TITLE     The receptor tyrosine kinase MuSK is required for neuromuscular
            junction formation in vivo
  JOURNAL   Cell 85 (4), 501-512 (1996)
   PUBMED   8653786
REFERENCE   8  (bases 1 to 8294)
  AUTHORS   Valenzuela,D.M., Stitt,T.N., DiStefano,P.S., Rojas,E., Mattsson,K.,
            Compton,D.L., Nunez,L., Park,J.S., Stark,J.L., Gies,D.R. et al.
  TITLE     Receptor tyrosine kinase specific for the skeletal muscle lineage:
            expression in embryonic muscle, at the neuromuscular junction, and
            after injury
  JOURNAL   Neuron 15 (3), 573-584 (1995)
   PUBMED   7546737
REFERENCE   9  (bases 1 to 8294)
  AUTHORS   Schultz,S.J. and Nigg,E.A.
  TITLE     Identification of 21 novel human protein kinases, including 3
            members of a family related to the cell cycle regulator nimA of
            Aspergillus nidulans
  JOURNAL   Cell Growth Differ 4 (10), 821-830 (1993)
   PUBMED   8274451
REFERENCE   10 (bases 1 to 8294)
  AUTHORS   Abicht,A., Muller,J.S. and Lochmuller,H.
  TITLE     Congenital Myasthenic Syndromes Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301347
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL513328.12 and AL157881.14.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Apr 8, 2019 this sequence version replaced NM_005592.3.
            
            Summary: This gene encodes a muscle-specific tyrosine kinase
            receptor. The encoded protein may play a role in clustering of the
            acetylcholine receptor in the postsynaptic neuromuscular junction.
            Mutations in this gene have been associated with congenital
            myasthenic syndrome. Alternatively spliced transcript variants have
            been described.[provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF006464.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374448.9/ ENSP00000363571.4
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-193               AL513328.12        28410-28602
            194-320             AL513328.12        42293-42419
            321-472             AL513328.12        46736-46887
            473-600             AL513328.12        55022-55149
            601-742             AL513328.12        56944-57085
            743-867             AL513328.12        93870-93994
            868-1027            AL513328.12        107260-107419
            1028-1034           AL513328.12        121821-121827
            1035-1298           AL157881.14        540-803
            1299-1474           AL157881.14        8508-8683
            1475-1498           AL157881.14        9352-9375
            1499-1700           AL157881.14        17535-17736
            1701-1892           AL157881.14        18247-18438
            1893-2041           AL157881.14        20410-20558
            2042-8294           AL157881.14        33026-39278
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q31.3"
     gene            1..8294
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="muscle associated receptor tyrosine kinase"
                     /db_xref="GeneID:4593"
                     /db_xref="HGNC:HGNC:7525"
                     /db_xref="MIM:601296"
     exon            1..193
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..2724
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /EC_number="2.7.10.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; muscle, skeletal receptor tyrosine-protein
                     kinase; muscle-specific kinase receptor; muscle-specific
                     tyrosine-protein kinase receptor; muscle, skeletal,
                     receptor tyrosine kinase"
                     /codon_start=1
                     /product="muscle, skeletal receptor tyrosine-protein
                     kinase isoform 1 precursor"
                     /protein_id="NP_005583.1"
                     /db_xref="CCDS:CCDS48005.1"
                     /db_xref="GeneID:4593"
                     /db_xref="HGNC:HGNC:7525"
                     /db_xref="MIM:601296"
                     /translation="MRELVNIPLVHILTLVAFSGTEKLPKAPVITTPLETVDALVEEV
                     ATFMCAVESYPQPEISWTRNKILIKLFDTRYSIRENGQLLTILSVEDSDDGIYCCTAN
                     NGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDSP
                     LRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEVFARILRAP
                     ESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRLQLFITK
                     PGLYTCIATNKHGEKFSTAKAAATISIAEWSKPQKDNKGYCAQYRGEVCNAVLAKDAL
                     VFLNTSYADPEEAQELLVHTAWNELKVVSPVCRPAAEALLCNHIFQECSPGVVPTPIP
                     ICREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHLLSVPECSKLPSMHWDPTACARL
                     PHLDYNKENLKTFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMTVIISIMSSFAIFV
                     LLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLL
                     SLEYPRNNIEYVRDIGEGAFGRVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQ
                     REAALMAEFDNPNIVKLLGVCAVGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSD
                     LSMRAQVSSPGPPPLSCAEQLCIARQVAAGMAYLSERKFVHRDLATRNCLVGENMVVK
                     IADFGLSRNIYSADYYKANENDAIPIRWMPPESIFYNRYTTESDVWAYGVVLWEIFSY
                     GLQPYYGMAHEEVIYYVRDGNILSCPENCPVELYNLMRLCWSKLPADRPSFTSIHRIL
                     ERMCERAEGTVSV"
     exon            194..320
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            321..472
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            473..600
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            601..742
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            743..867
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1027
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1028..1034
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1298
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1474
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1475..1498
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1700
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1892
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2041
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..8294
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4675..4680
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="hexamer: AATAAA"
     polyA_site      4699
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="major polyA site"
     regulatory      7501..7506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="hexamer: AATAAA"
     polyA_site      7522
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="major polyA site"
     regulatory      7588..7593
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="hexamer: ATTAAA"
     polyA_site      7608
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="major polyA site"
     regulatory      8033..8038
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="hexamer: AATAAA"
     polyA_site      8056
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="major polyA site"
     regulatory      8264..8269
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
                     /note="hexamer: AATAAA"
     polyA_site      8294
                     /gene="MUSK"
                     /gene_synonym="CMS9; FADS; FADS1"
ORIGIN      
        1 agtcattagc agacaaccct tttgcaacaa agtatgcttt aaaatgtaaa ctgtggagcc
       61 attttccttg cgttgtccag aaggaacttc gtcctgcgtg agcctggatt aatcatgaga
      121 gagctcgtca acattccact ggtacatatt cttactctgg ttgccttcag cggaactgag
      181 aaacttccaa aagctcctgt catcaccact cctcttgaaa cagtggatgc cttagttgaa
      241 gaagtggcta ctttcatgtg tgcagtggaa tcctaccccc agcctgagat ttcctggact
      301 agaaataaaa ttctcattaa actctttgac acccggtaca gcatccggga gaatgggcag
      361 ctcctcacca tcctgagtgt ggaagacagt gatgatggca tttactgctg cacggccaac
      421 aatggtgtgg gaggagctgt ggagagttgt ggagccctgc aagtgaagat gaaacctaaa
      481 ataactcgtc ctcccataaa tgtgaaaata atagagggat taaaagcagt cctaccatgt
      541 actacaatgg gtaatcccaa accatcagtg tcttggataa agggagacag ccctctcagg
      601 gaaaattccc gaattgcagt tcttgaatct gggagcttga ggattcataa cgtacaaaag
      661 gaagatgcag gacagtatcg atgtgtggca aaaaacagcc tcgggacagc atattccaaa
      721 gtggtgaagc tggaagttga ggtttttgcc aggatcctgc gggctcctga atcccacaat
      781 gtcacctttg gctcctttgt gaccctgcac tgtacagcaa caggcattcc tgtccccacc
      841 atcacctgga ttgaaaacgg aaatgctgtt tcttctgggt ccattcaaga gagtgtgaaa
      901 gaccgagtga ttgactcaag actgcagctg tttatcacca agccaggact ctacacatgc
      961 atagctacca ataagcatgg ggagaagttc agtactgcca aggctgcagc caccatcagc
     1021 atagcagaat ggagtaaacc acagaaagat aacaaaggct actgcgccca gtacagaggg
     1081 gaggtgtgta atgcagtcct ggcaaaagat gctcttgttt ttctcaacac ctcctatgcg
     1141 gaccctgagg aggcccaaga gctactggtc cacacggcct ggaatgaact gaaagtagtg
     1201 agcccagtct gccggccagc tgctgaggct ttgttgtgta accacatctt ccaggagtgc
     1261 agtcctggag tagtgcctac tcctattccc atttgcagag agtactgctt ggcagtaaag
     1321 gagctcttct gcgcaaaaga atggctggta atggaagaga agacccacag aggactctac
     1381 agatccgaga tgcatttgct gtccgtgcca gaatgcagca agcttcccag catgcattgg
     1441 gaccccacgg cctgtgccag actgccacat ctagattata acaaagaaaa cctaaaaaca
     1501 ttcccaccaa tgacgtcctc aaagccaagt gtggacattc caaatctgcc ttcctcctcc
     1561 tcttcttcct tctctgtctc acctacatac tccatgactg taataatctc catcatgtcc
     1621 agctttgcaa tatttgtgct tcttaccata actactctct attgctgccg aagaagaaaa
     1681 caatggaaaa ataagaaaag agaatcagca gcagtaaccc tcaccacact gccttctgag
     1741 ctcttactag atagacttca tcccaacccc atgtaccaga ggatgccgct ccttctgaac
     1801 cccaaattgc tcagcctgga gtatccaagg aataacattg aatatgtgag agacatcgga
     1861 gagggagcgt ttggaagggt gtttcaagca agggcaccag gcttacttcc ctatgaacct
     1921 ttcactatgg tggcagtaaa gatgctcaaa gaagaagcct cggcagatat gcaagcggac
     1981 tttcagaggg aggcagccct catggcagaa tttgacaacc ctaacattgt gaagctatta
     2041 ggagtgtgtg ctgtcgggaa gccaatgtgc ctgctctttg aatacatggc ctatggtgac
     2101 ctcaatgagt tcctccgcag catgtcccct cacaccgtgt gcagcctcag tcacagtgac
     2161 ttgtctatga gggctcaggt ctccagccct gggcccccac ccctctcctg tgctgagcag
     2221 ctttgcattg ccaggcaggt ggcagctggc atggcttacc tctcagaacg taagtttgtt
     2281 caccgagatt tagccaccag gaactgcctg gtgggcgaga acatggtggt gaaaattgcc
     2341 gactttggcc tctccaggaa catctactca gcagactact acaaagctaa tgaaaacgac
     2401 gctatcccta tccgttggat gccaccagag tccatttttt ataaccgcta cactacagag
     2461 tctgatgtgt gggcctatgg cgtggtcctc tgggagatct tctcctatgg cctgcagccc
     2521 tactatggga tggcccatga ggaggtcatt tactacgtgc gagatggcaa catcctctcc
     2581 tgccctgaga actgccccgt ggagctgtac aatctcatgc gtctatgttg gagcaagctg
     2641 cctgcagaca gacccagttt caccagtatt caccgaattc tggaacgcat gtgtgagagg
     2701 gcagagggaa ctgtgagtgt ctaaggttga agacgttcaa ataaaatgct gcagtttcct
     2761 ctcagactct gtgagccagg ggaatcctac accagaggcc caacaagacc cacataggaa
     2821 agagtaagag taaacatgag tagtgtgttg tttgcttccc agggagagca aagacagtgc
     2881 aaaacccatg tggtagacgg acccattgaa agccagtgat tggaaacaca ggctaggaaa
     2941 tgtgtcagat aatggagaca actattcttc ttcatgaagg tttgtaatac acactgcaca
     3001 gggaagaatg tcatcctgtt cagtttccaa agtagctggt cacagagtaa agctctgctg
     3061 tattaaattt taatatgaaa tgggattcag agcttcattt tttaattgag ttaattcctg
     3121 tccattgtga aagtagctta atcgtcatgt aagacactta ggtgaagtac agaaaactaa
     3181 aaagaaggaa aagccactca ttctgtctcc ccccaaagac gagtcttagt gttttattaa
     3241 atctttcttg catttaatgc ataattttat atttgtttgt attctgcaga gttgtggtta
     3301 cattttattg atagagggtt tctaaggaat gaaacagcaa atcagcaagc ctgtaactct
     3361 gagttccctt ttctgtcctg tgcccctcct tttttccttt ctgtgcattt gtcatgaatt
     3421 aagtctgctt attttattcc tccagttttc ttgaaacaag tttttgtatc ttactgattt
     3481 accttttcct ttaaaaattg tattggtatc ttttccattg ctttaatctt tatttcttct
     3541 tctttccagg acttctgtcc ttttttagtt attaagctat ttttttcttt tatttcattc
     3601 atgtaataaa cactctggca gaaataataa cattttaaat accattgtag attgatatgt
     3661 atatgccttt tgcaaagtat tactattttt cctgcatttg cctttgaagc cacacatttc
     3721 aacattctca cctattaata ttattactag agtctattta aatctcttaa accataattt
     3781 ataatatgag atttcacaag atacacttgt ggctctgagt tatttcaaaa ctgatatttc
     3841 tgtgcattct gacattttat tccttttaga aacaaattca gttggctgta gaattaatat
     3901 cgttctgctt aacaactttg tgcatgacat attaactgat tttgttgtat ttcatgattt
     3961 aaaaaaaaaa gcagcagctc tggaactcag attcctgggg gttcagattt tttctcagtg
     4021 gatcctcaca caatacatct ttatctaagt ccatacatga tctgagttta tttctgatat
     4081 agcaggaacc caaattctcc tgaaaacgaa cagggaagag aaaataacag tctactctat
     4141 accagcttat cttaattcag actaattggt agttagggca ttggaggaat ggtgtgaatt
     4201 cttgaaagta gattttcgct gtctgctgga aaactctgcc tgaaatcccc aaatctgcct
     4261 catgtaacag cttgacatct ttggtgggaa acctgccatt tcttattcat agtgtctcca
     4321 ggcaaccaag acaaaaatgt agaaattttc ctagactttg ccctctcttt catcatcagt
     4381 tttctattag ttttacttcc taaaatcctc ctgctgacat cctcttccct tcaacccaag
     4441 agccaatgac tcaacctcta ccgttgccca ccctgtctat tagctggcct tcctctcatt
     4501 tcagacctga acagattctg taggatacaa cgacagtaag gcggtcctta gtcacagttc
     4561 tggttcataa atagcgaggt cagcatttag gactgagacc taaagaagtg cttcagataa
     4621 aaatcatgag tctaagtcca aaattgatac tgtaatccaa atgactgaat aaagaataaa
     4681 gttgcagcgt gtgtctaaag cagaaagtca agatgccaac aaagcagaaa agttggagag
     4741 ggccaccagc aagtctagaa cttgagcata gatatccatg gcttatttta gatggttcat
     4801 gtatgctctg caaaattcaa agacagcttc gggctttctc cgctgataaa gttttgttgt
     4861 aaaagacaga catagaagtc aggctgacag aaaatagagt aacagtaatc ataaaaatta
     4921 taatatcagc tagcatttat tgaaatctta ccatgtgtat tctgaggcct ttaaatatat
     4981 gaacttaaac ctcacaacat ccctgtaatg ttggtactat cattatatac attttagttt
     5041 tgctttgttt tgttttgttt ttgagacgga gtctcgctct gtcgcccagg ctggagtgca
     5101 gtggcacgat cttggctcac tgcaagctca gcctccaaca gtcacagaga gattgtgcaa
     5161 ctggcccgag tgcacacagc aacagagcct actttttgac caaaagaggc cgaattctca
     5221 accactatac tgtagagcct ctgtacatga tagaagcaat aactgaacct cagtaactct
     5281 gtggaagtga tttgtaattt gcatgtcgtt tcaagcacac tctatatcta tattttattc
     5341 tattaacttc ctaagtcttt ttaatgaata gcaaaaaaag tatatttcag cattatcaat
     5401 ttaagttaat cataacatct aataggtgac atgtttattt tttcatgagt tctaattaca
     5461 ttttcaagtt aagatcactg acttattccc taagtgctcg atctaatgtt ctgaatcaca
     5521 ggagtataga tgcattattc accaatattg tttacttttt taaataagat tttttttcat
     5581 ggtagaatca tcatatacat tcaaaaaggt agacaaaaga gagacactca cagtgtcact
     5641 gttaccaagt gccaggacag atatcagtaa ggcaccagca gtcattttta gtgatcaaat
     5701 accaaacatg gtaagatacc agagtatttt tcacatgaca cccaaaaaca tattctctgc
     5761 tatcgtggta gagcagaaaa agaacacttt cttttcattt tacagtatat tacgaacctg
     5821 ttccacgtca ttaaatattc ttctaaaaac ccaattttgg aataatattc tattttattc
     5881 atatactgta atttagctaa tcaatcttgt atttttgaac atttaggttt tttccaattt
     5941 ttatcattat aaaaaatgct gtaattaaca tccttatttg ttcatgtcca catttctttt
     6001 tatgaaaatt cagagaaagg aaatgtctgg accaaatggc atgtacaata ttaacacttc
     6061 tgttatgtgg tttcatattc tgcagaaata ttactgccac ttctacattg tacaagagat
     6121 ctcatagccc taaacatttg ctttcataga taattttctg ttttttagaa atcttttgtc
     6181 tctttggtta tagaaaaaga gaacctcttt ttaattttat tttttaaatt actagtcagg
     6241 ttgaatgttt cttgttccta ggtcattaac agtttttatt ttggcaattg tctacagccc
     6301 atttttctgc tgtgttcatt ttctaatcga attatgggat ttatcttgta aaaagcctat
     6361 taagcctttt tcatacttta cacaagagat cacatctact tactttacct taaaataaca
     6421 tttacttatt ttcttataaa agtaatatat tatgtttgaa aagaaaaata atatgcaata
     6481 atatacaata atatgtataa tcataataga caataataaa aatgaaaaaa caccaaccag
     6541 aggtaattaa cattaacatc ataatattct tccaggcttt ttcagataga caggtaggta
     6601 agtaggttgg taggtaaata gatagataga tgatacatag agagagatag atagataatt
     6661 taaacaaaaa taagatcata ctgctacatt acttcaagcc ttgcttttat cacttaatat
     6721 atcttaaaca tcttctacat gaacaagtat tcatgtgcaa cattaatttt gatggttgaa
     6781 tagtattata cagatttatc gtaattgaca taactattct actgttagac atttggttta
     6841 tttctatttt tccattataa aatgtaatgt ttggatcatc ctgttgctaa attttgtaag
     6901 catttatctt aagtgatttc tttagctata actcctagaa gtgaaatttc tagattaaag
     6961 gataaatgca cttttaatac tttttaaata tattaaaaaa ttaccttcct gaattatttt
     7021 attaacatgt acctctccta gtcagataag acagcccatt tatttcagtc tcaccaatac
     7081 tgagtatagc attttaatca ttgccaatat aatagctaat agcttaaagt tatttcatag
     7141 cttatattta cacttcttta aatactaata aaattgaaca ttgtttaatg ttttgaggat
     7201 acttgattgg tgatttgcct cctactatta tttttctact gggtatatct ttttcttgtt
     7261 gctttgtaag agcactttat atattaagga gttaaatttt gtgatatatg tttcaactac
     7321 ttttatcatg ttaaaaatat agacttctat ttgtctttta ttgtaggttt tatcaatcag
     7381 aaaagaatgt tgacttttcc gtccattcag atgattgtgg cttttttccc taatggattt
     7441 gttaatatgt tcaattgtat gaatttattt cctaatgtag cgatatcctt gtcttctagg
     7501 aataaacttt ttgggcgaat catatcaatc ttctacttaa ctaccaaatt aatttacata
     7561 tatttgcatc aatattaagt tcataaaatt aaaagtttat cattttcatc tctgttagag
     7621 ttttgtattg gcattagcat ttgtattagc attgtattag gaaaaactca ggagtttttc
     7681 tctataatct tggttagttt atttttaaat aattgttatt tatgaaagta atacataaaa
     7741 atgttgttta aaaaaactca caagagggta aaaattaaaa aaataataaa agttcccttc
     7801 ttccttttca tctctagtcc cagcctaagt gctaaccact tcacccattt atagtgttag
     7861 ttcttctggt gattaccagt gcaattctaa caaataggtt tgcatttctc tttctgaatt
     7921 gatcaatttt ttaaaaatat gcagcactct gactcaaact ccctttcttt ttcctgagat
     7981 atgttaactt ctaatgttat attgtttatc acaactttca caaatatttc taaataaagg
     8041 ttacctactg attctggatt gtgctacata aaattagagc ttccaaattt ccctccatca
     8101 cttctccctg ctctctactc tcacaatcag tcaactatac acctatttct acgctgtcaa
     8161 ctatatgttc atttctatga atctgtaata tttgcatttt gttctggaac aaattaagat
     8221 ttaggtgctt tgactagatt gattctaaac atcaaaaaac aaaaataaaa tatctgcaat
     8281 tatgtgatca tgta
//
