LOCUS       NM_012123              10698 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial tRNA translation optimization 1 (MTO1),
            transcript variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_012123
VERSION     NM_012123.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Zhou,C., Wang,J., Zhang,Q., Yang,Q., Yi,S., Shen,Y., Luo,J. and
            Qin,Z.
  TITLE     Clinical and genetic analysis of combined oxidative phosphorylation
            defificiency-10 caused by MTO1 mutation
  JOURNAL   Clin Chim Acta 526, 74-80 (2022)
   PUBMED   34990597
  REMARK    GeneRIF: Clinical and genetic analysis of combined oxidative
            phosphorylation defificiency-10 caused by MTO1 mutation.
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Li,D., Zhang,J., Yang,J., Wang,J., Zhang,R., Li,J. and Zhang,R.
  TITLE     CircMTO1 suppresses hepatocellular carcinoma progression via the
            miR-541-5p/ZIC1 axis by regulating Wnt/beta-catenin signaling
            pathway and epithelial-to-mesenchymal transition
  JOURNAL   Cell Death Dis 13 (1), 12 (2021)
   PUBMED   34930906
  REMARK    GeneRIF: CircMTO1 suppresses hepatocellular carcinoma progression
            via the miR-541-5p/ZIC1 axis by regulating Wnt/beta-catenin
            signaling pathway and epithelial-to-mesenchymal transition.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10698)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 10698)
  AUTHORS   Ji,N., Wang,Y., Gong,X., Ni,S. and Zhang,H.
  TITLE     CircMTO1 inhibits ox-LDL-stimulated vascular smooth muscle cell
            proliferation and migration via regulating the miR-182-5p/RASA1
            axis
  JOURNAL   Mol Med 27 (1), 73 (2021)
   PUBMED   34238206
  REMARK    GeneRIF: CircMTO1 inhibits ox-LDL-stimulated vascular smooth muscle
            cell proliferation and migration via regulating the
            miR-182-5p/RASA1 axis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10698)
  AUTHORS   Wang,N., Cao,Q.X., Tian,J., Ren,L., Cheng,H.L. and Yang,S.Q.
  TITLE     Circular RNA MTO1 Inhibits the Proliferation and Invasion of
            Ovarian Cancer Cells Through the miR-182-5p/KLF15 Axis
  JOURNAL   Cell Transplant 29, 963689720943613 (2020)
   PUBMED   32731816
  REMARK    GeneRIF: Circular RNA MTO1 Inhibits the Proliferation and Invasion
            of Ovarian Cancer Cells Through the miR-182-5p/KLF15 Axis.
REFERENCE   6  (bases 1 to 10698)
  AUTHORS   Krull,M., Brosius,J. and Schmitz,J.
  TITLE     Alu-SINE exonization: en route to protein-coding function
  JOURNAL   Mol Biol Evol 22 (8), 1702-1711 (2005)
   PUBMED   15901843
REFERENCE   7  (bases 1 to 10698)
  AUTHORS   Umeda,N., Suzuki,T., Yukawa,M., Ohya,Y., Shindo,H., Watanabe,K. and
            Suzuki,T.
  TITLE     Mitochondria-specific RNA-modifying enzymes responsible for the
            biosynthesis of the wobble base in mitochondrial tRNAs.
            Implications for the molecular pathogenesis of human mitochondrial
            diseases
  JOURNAL   J Biol Chem 280 (2), 1613-1624 (2005)
   PUBMED   15509579
REFERENCE   8  (bases 1 to 10698)
  AUTHORS   Bykhovskaya,Y., Mengesha,E., Wang,D., Yang,H., Estivill,X.,
            Shohat,M. and Fischel-Ghodsian,N.
  TITLE     Phenotype of non-syndromic deafness associated with the
            mitochondrial A1555G mutation is modulated by mitochondrial RNA
            modifying enzymes MTO1 and GTPBP3
  JOURNAL   Mol Genet Metab 83 (3), 199-206 (2004)
   PUBMED   15542390
  REMARK    GeneRIF: Phenotype of non-syndromic deafness associated with the
            mitochondrial A1555G mutation is modulated by mitochondrial RNA
            modifying enzymes MTO1 and GTPBP3.
REFERENCE   9  (bases 1 to 10698)
  AUTHORS   Finnila,S. and Majamaa,K.
  TITLE     Lack of a modulative factor in locus 8p23 in a Finnish family with
            nonsyndromic sensorineural hearing loss associated with the 1555A>G
            mitochondrial DNA mutation
  JOURNAL   Eur J Hum Genet 11 (9), 652-658 (2003)
   PUBMED   12939650
REFERENCE   10 (bases 1 to 10698)
  AUTHORS   Li,X., Li,R., Lin,X. and Guan,M.X.
  TITLE     Isolation and characterization of the putative nuclear modifier
            gene MTO1 involved in the pathogenesis of deafness-associated
            mitochondrial 12 S rRNA A1555G mutation
  JOURNAL   J Biol Chem 277 (30), 27256-27264 (2002)
   PUBMED   12011058
  REMARK    GeneRIF: identification of full-length cDNA and elucidation of
            genomic organization of the human MTO1 homolog
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DN993156.1, AK074625.1,
            BC011051.1, BE501391.1 and AL603910.6.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_012123.3.
            
            Summary: This gene encodes a mitochondrial protein thought to be
            involved in mitochondrial tRNA modification. The encoded protein
            may also play a role in the expression of the non-syndromic and
            aminoglycoside-induced deafness phenotypes associated with a
            specific mutation in the mitochondrial 12S rRNA gene. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the predominant
            isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.109043.1,
                                           SRR1803616.40325.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000498286.6/
                                                  ENSP00000419561.2
            RefSeq Select criteria             :: based on manual assertion,
                                                  conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DN993156.1         168-182
            16-2382             AK074625.1         1-2367
            2383-2558           BC011051.1         2330-2505
            2559-2918           BE501391.1         5-364               c
            2919-10698          AL603910.6         94971-102750
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q13"
     gene            1..10698
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="mitochondrial tRNA translation optimization 1"
                     /db_xref="GeneID:25821"
                     /db_xref="HGNC:HGNC:19261"
                     /db_xref="MIM:614667"
     exon            1..335
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     CDS             119..2197
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="isoform a is encoded by transcript variant 2;
                     homolog of yeast Mto1; protein MTO1 homolog,
                     mitochondrial; mitochondrial MTO1-3; mitochondrial
                     translation optimization 1 homolog"
                     /codon_start=1
                     /product="protein MTO1 homolog, mitochondrial isoform a"
                     /protein_id="NP_036255.2"
                     /db_xref="CCDS:CCDS34485.1"
                     /db_xref="GeneID:25821"
                     /db_xref="HGNC:HGNC:19261"
                     /db_xref="MIM:614667"
                     /translation="MFYFRGCGRWVAVSFTKQQFPLARLSSDSAAPRTPHFDVIVIGG
                     GHAGTEAATAAARCGSRTLLLTHRVDTIGQMSCNPSFGGIGKGHLMREVDALDGLCSR
                     ICDQSGVHYKVLNRRKGPAVWGLRAQIDRKLYKQNMQKEILNTPLLTVQEGAVEDLIL
                     TEPEPEHTGKCRVSGVVLVDGSTVYAESVILTTGTFLRGMIVIGLETHPAGRLGDQPS
                     IGLAQTLEKLGFVVGRLKTGTPPRIAKESINFSILNKHIPDNPSIPFSFTNETVWIKP
                     EDQLPCYLTHTNPRVDEIVLKNLHLNSHVKETTRGPRYCPSIESKVLRFPNRLHQVWL
                     EPEGMDSDLIYPQGLSMTLPAELQEKMITCIRGLEKAKVIQPGYGVQYDYLDPRQITP
                     SLETHLVQRLFFAGQINGTTGYEEAAAQGVIAGINASLRVSRKPPFVVSRTEGYIGVL
                     IDDLTTLGTSEPYRMFTSRVEFRLSLRPDNADSRLTLRGYKDAGCVSQQRYERACWMK
                     SSLEEGISVLKSIEFLSSKWKKLIPEASISTSRSLPVRALDVLKYEEVDMDSLAKAVP
                     EPLKKYTKCRELAERLKIEATYESVLFHQLQEIKGVQQDEALQLPKDLDYLTIRDVSL
                     SHEVREKLHFSRPQTIGAASRIPGVTPAAIINLLRFVKTTQRRQSAMNESSKTDQYLC
                     DADRLQEREL"
     exon            336..535
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            536..653
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            654..943
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1056
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1247
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1378
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1583
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1755
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..1874
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..2035
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..10698
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2539..2544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="hexamer: ATTAAA"
     polyA_site      2558
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="major polyA site"
     regulatory      2900..2905
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="hexamer: CATAAA"
     polyA_site      2922
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
     regulatory      4001..4006
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="hexamer: AATACA"
     polyA_site      4023
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
     regulatory      5265..5270
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="hexamer: ATTAAA"
     polyA_site      5292
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
     regulatory      10678..10683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
                     /note="hexamer: AATGAA"
     polyA_site      10698
                     /gene="MTO1"
                     /gene_synonym="CGI-02; COXPD10"
ORIGIN      
        1 gtcctacccc gtgatattaa agcaagatgg ccgcgccctg cagattgtct cttgttgcgt
       61 aagttttttt gaccgtcact cgtgtcagct tcaaagtcag atagattttt ctcccagcat
      121 gttctacttc cgaggctgtg gccgttgggt cgcggtttcc ttcaccaagc agcaatttcc
      181 gttggcacgg ttgagcagtg acagcgcggc gccccggact ccgcacttcg acgtgatagt
      241 cattggtgga ggacatgccg ggactgaggc agccaccgcc gccgctcggt gcggctctcg
      301 gactctgctc ctcactcacc gcgtggacac gatcggtcag atgtcatgta atccttcctt
      361 tggtggcatc ggaaagggac atttaatgag ggaagtagat gccttggatg gcctgtgttc
      421 tcgcatctgt gaccagtctg gtgtacatta taaagtatta aaccggcgta agggaccagc
      481 tgtgtggggt ctgagagctc agattgatag gaaactctat aaacagaaca tgcagaaaga
      541 aatcttgaat acaccactgc ttactgttca ggagggagct gtagaagatc ttattcttac
      601 agaaccagag cctgaacaca ctgggaaatg ccgtgtcagt ggggttgttt tggtggatgg
      661 aagcacagta tatgcagaga gtgtgattct gactactggg acatttctga gaggcatgat
      721 tgtaattgga ttggagacgc atccagcagg acgtttaggg gatcagcctt ctataggatt
      781 ggctcagaca ctggagaagt tagggtttgt ggtgggaagg ttgaagactg ggactccacc
      841 ccgaattgcc aaagagtcca ttaatttcag tattctaaac aagcatatac cggacaatcc
      901 atccatacca ttcagcttta ccaatgagac agtatggatt aagccagaag atcagctgcc
      961 atgttacttg actcacacca accctagagt ggatgagatt gtccttaaga accttcacct
     1021 taatagtcat gttaaagaaa cgacaagagg acctcgatac tgtccctcca ttgaatcaaa
     1081 agttttgcgt tttccaaacc gtctacatca ggtttggttg gaacctgaag gaatggattc
     1141 tgaccttatc tacccacagg ggttatctat gacgctacca gctgagttac aagagaaaat
     1201 gatcacatgc atcagaggct tggagaaagc taaagtgatt cagccaggct acggtgttca
     1261 gtatgattac ttagatcccc gtcagatcac cccttccttg gagactcatt tggttcaacg
     1321 actcttcttt gctggacaga tcaatggcac cactggttat gaggaagctg cagctcaagg
     1381 tgtgatagcc ggaatcaacg ccagtcttcg ggtcagtcgc aagcctccct ttgtggttag
     1441 ccgaacagaa ggttacatag gagtcttgat tgatgacctc actactctgg gcaccagtga
     1501 accataccgc atgtttacca gccgagtaga gttccgtttg tcactgcgcc ctgataatgc
     1561 tgacagccgg ctcacactgc gagggtataa agacgctggc tgtgtgtccc aacaacgata
     1621 tgaaagagct tgttggatga agtcttcttt agaagaaggc atttctgtgt tgaaatctat
     1681 tgagtttttg agctctaaat ggaaaaaatt aatcccagag gcttctataa gtactagtag
     1741 aagtctgcct gtcagagctc tcgatgttct gaagtatgag gaagttgaca tggattcatt
     1801 agccaaggct gttccagagc ccttgaagaa gtatactaaa tgtagagagc tggctgaaag
     1861 actgaaaata gaagccactt atgaatcagt gttgttccat caactacaag aaataaaggg
     1921 agttcagcaa gatgaagctc tccaactgcc aaaagaccta gattatttga ctatcaggga
     1981 tgtgtctttg tcccatgaag ttcgagagaa actacatttt agtcgtccac agacgatcgg
     2041 ggctgctagt cgcatacccg gagtaacacc tgccgccatc atcaatctgc tgagatttgt
     2101 gaagaccact caacgaagac agtcggctat gaatgaatca tccaagactg atcaatactt
     2161 atgtgatgca gacagacttc aagagagaga gttatagctt tcaattcata aaagattttt
     2221 aaagagcata taaataattt gatcaataca acagtataga taaaagaatt atttagcaca
     2281 tgttaaaata gctttattag gttactatgg gtttgccatt aatttctgag tgggacagaa
     2341 attataattg tgctttttcg tgtatatgaa aaaactagtc gtaaacaatt tgtactcttt
     2401 ctttaaggag ctgtaataca aataactttg tgcagtgttc atcaaagaga gagacagtga
     2461 acctaaaact gaacctggaa taaaactcaa catgcagatt tgcctactca tagggacttt
     2521 gcctattaag tctaccaaat taaaagtctt atcattcagc gtgttttgag agttaataat
     2581 cttttgcttg gttatactag gctaggaagc aatatcaaag cccttaaatt ctcaagacta
     2641 aagacttgag attatataaa attatccaga atcattcagc taatactatt ggaatatagt
     2701 atgtagggtg aaagttgaca gaaatctgta cttcttcagg aaatcctcac tggtaattgt
     2761 tttgaacatt aatgaaagcc ctataaaata gaaagattgg aaatcaccat tgtttcctat
     2821 tatccacagt gtggatggat acttatttgg tgacctagtt atgggagaat tgagtaacgt
     2881 tagttatttt atatcatacc ataaaaatat tttcttaaga aattgtcatg gccatcattt
     2941 cttaataatg aagacaggtc catagtaact ggtgagtggt gtttccctgt ttttaagtca
     3001 gaatgacaga aaaaacagaa tagtggtagt acccctcttc ctctccagta cttcaatggt
     3061 atgtcttcac aggggttaga gtgggataat cagctgggcc ttttgactca gatacctcag
     3121 atacctcaac cattctaaat agactaaggg atgaatacag ccaaatgggc cagacagccc
     3181 tgcttgtctc tggaattcca acccagatct ctggtgtcag cctagggaga agctcccgtg
     3241 tcaccttcgg gccaaagcag gtactcagga gagttctaag ggccccaaag tcactttggc
     3301 tacagatgtg tatgttaagc ctgattatgg gtgtgaggca accaagtatc ttgttactat
     3361 aattgcatat ggccacttgg aggtagctta gttttcttgg gtttatttta tctaatttta
     3421 gtctttccag aaagcagtaa gattagtagc aagtaaaaag tatttgtagt gctggataat
     3481 ttaggaatga tgaatatttt ttaatacttg attcagtgat ttcaaaaggt cgtaactttt
     3541 agtcatagct actcctgggc aaaattcttc ttacagagac aatattaatt acaataccac
     3601 attgtgtata tattttgaaa agaggctggt tgcagcagct cacgcctgta atcccagcac
     3661 tttgggaggc tgaggcgggt ggataacctg aagtcaggag ttcaagacca gcctggccaa
     3721 catggcaaaa ccccatctcc actaaaaata ccaaaattag ccaggcgtgg tggagcaagc
     3781 ctgtagtccc agctactcag gaggctgagg caggagaatc gcttgaaccc aggaggcaga
     3841 ggttgcagtg agctgagatt gtgccactgc actccagcct gggcgacaga gtgagactgt
     3901 ttcaaaaaaa agaaaaagaa aagaaggtat tttgtgtttt tgtgctaccc gttcagcaaa
     3961 ataatgaaat tcattgtttc taaacttttt tttctgatta aatacaagag taaaaaaagt
     4021 gaatggagtg ccttttgaat agaatataac aaaaatttat cttaaagaaa cgggaagtaa
     4081 cctggcacag tggctcacgc ctgtaatccc agcactttgg gaggctgagg caggtggatc
     4141 acgaggtcag gagatcgaga ccatcctgcc caacatggtg aaaccccatc tctactaaaa
     4201 atacaaaaat tagctaggtg tgttggcacg tgcctgtagt cccaactact caggaggctg
     4261 aggcaagaga atcacttgaa ctcaggaggc agaggttgca gtgagccaag atcatgccac
     4321 tgcactccag cctggcaaca gagtgagact ctgtctcaaa aaaaaaaaaa gaaacaggaa
     4381 gttcaaatat tttgggaggt tcttgacagc tacaatttgg ggctagtgac actatacaca
     4441 tataaagaaa ctttaaagtt tcttagtaat gaaggtttgt aatgctattc cattacaata
     4501 gtaactgtct cactgaaaaa tgtttcatgg aaaatacata ttttagattc tatgatttgc
     4561 tttaggatgt gaacttcttt attttttaga gacagggtct cactctgttg cccaggctgt
     4621 agtgcagtgg tacaatcata gctcactgca gccttgatct cctgggctca agtgatcctt
     4681 atgcctcagc ctcccaagtg gctgggacta caggcacatg ccaccatgcc tggctaagtt
     4741 ttaaaaaaat tttttgtagg aatgaagtct cactatgttg cccaggctgg tcttgaactt
     4801 ctggcctcaa atgatcctct tgccttggcc tcccaaagtg ctcagattac aggcatgagc
     4861 caccacacgt ggcctgaaac ctctttaaat ccacaatgtt tgtatgttaa cttaaacaaa
     4921 attacatcac ttaatatgtc aaaacagcct accctctggt gctgctatgc agaagtacta
     4981 cagaatatga tgccctagga agaaatcagc taaaaatgta accttactca atgtagttac
     5041 ttcatagaaa tcccatcact gacaataatc agctgccaac gaaagctggc catacttgtg
     5101 acttcctcta ttcaccacag accagactgc ctaccagttg aaaaagccag gtattttttc
     5161 aattcaattc aacaggaaca ccaaaaaata agaaatgact aagaaacact aagcatttgt
     5221 aaaattactg ggggaggaag gatgaagata cgaacctatg ctaaattaaa cgattatggc
     5281 cttgaaaatg aagttccttt atagccaaga gcttaaattt tgttactgat ggttttaaaa
     5341 gtattttgtc attttttagt ttcaacctag attggaatag ccatggcttt tctacttcag
     5401 aaatttcaac tcctagaaga atgccctgtc cagaagaagt ggtcaggagt atcaggaaag
     5461 ccaccagcag agactagggg tttattctga tctggaagag ccacttaaca ctggaatgca
     5521 cacagtgaaa attaattgtt cctcttacac tccctctcca caatacttct attattacac
     5581 ttacttcgtt ctaccttaat gtttttattt agagacaaga tctcactctg ccaccctggc
     5641 tggagtgcag tggcatgatt acagctcact gcagcctcaa cctccctggc tctagctatt
     5701 ctcccatctc agccctccaa gtagctggga ctacaggtgt gcaccaccat gcacggctaa
     5761 tttttaagtt tttgtagata tggggtctta ctatgttgcc caggctggtc tcaaagtcct
     5821 gggctcaatt aatcctccct cttcagcctc ctgtgtaggt gatagtacag gcatgagcca
     5881 ccatgtgcct ggccctacct taatgttaat atctgtctac cttaagaaga tataagcctt
     5941 gtaaatataa gcaatatcac agttgtagat actcgtgctt tttaaataga ttaatgaata
     6001 ctactaaagg caagaaatgg tattactaaa actcaagtga ataattaaat gagatcaaaa
     6061 tatttgtgac aggccaggca cggtggctta cgcctgtaat cccagcactt tgggaggcca
     6121 ggcggatcac ttgagctcag cagttcaaga ccagcctggg caacatggcg aaactccgtc
     6181 tttaccaaaa atacaaaaat aagccaagca tggtggcacg tacctgttgt tccagcaact
     6241 tgggggctga ggcaggagaa tcacttgagc cggggagttt gaggctgcag tgagttgata
     6301 gcatgccacc acactccagc ctgggcaaca gagtgagacc ctgcctgaaa aaaagcccag
     6361 gtgtggtggc tcacatctgt aatcccagca ttttgggatt acaaatctgc cagcgcaagc
     6421 agatcacttg aggataggag tttgagacca gcctgaccaa catgttgaaa ccccatctct
     6481 tattaaaata caaaaattag ctgggcgtgg tggcatgcgc ctgtaatccg agctactcag
     6541 gtggctgagg cacaagaatc gcttgaacct gggagatgga agttgcaatg agctgagatc
     6601 aagccactgc actccagcct gggcaacaga gcaagactgt ctcaaaaaaa aaaaatttgt
     6661 acagtcatgt tcaaagtagc attaattcac aatagccaaa agctggaagt agcccaagta
     6721 tccaccaatg aatgaaagga taaacaaaat gtggcatata catacaatgg aatattatac
     6781 agccttaaaa aggaaggaaa ttcatataca tgctacagca tggataaacc ttgaaaaagt
     6841 tatgctaact gaaatcagcc agtcacaaaa agacaaatac tgtatgattc cctttataag
     6901 agttacctgg agcactcaaa ttcatagaga cagtaaaatg atggttacca ggggatgggg
     6961 gagggagaaa caggaaacta ttctttagtg agtacagaat ttcagttttt ttgttttgtt
     7021 tttgtttttg tttttgagat ggagcctcac actgtcaccc aggctggagt gcaatggtgt
     7081 gatcttggtt cactgcaacc tctgcctcct aggttcaaat gattcttctg cctcagtctc
     7141 ctgagtctgg gattacaggc gcctgccatc atgcccagct aatttttgta tttttaatag
     7201 agacagggtt tcacaggttg gccaagctag tctcgaactc ctgacctcat gatccgccca
     7261 cctcgacctc ccaaagtgct gggattacag gggtgagcca ccatgcccag tccagaattt
     7321 caagttttgc aagatgaaaa gagttctgga gatggttgat ggtgatggtt gcacaacaat
     7381 ttgaatattt taaatgccaa tgaactgttc atttacaaat ggttacgatg gaatttttct
     7441 ttttcttttt tttttcttga gacggagtct cgctctgtca ctcaggctag agtgcagtgg
     7501 cactatctcg gctcactgca acctcctcct gctgggttaa agtgattctc ttgcctcagc
     7561 ctcccgagca gctgagacta caggcgcgtg ccaccatgct cagctaattt tttctatttt
     7621 tagtggagac agggtttcac catgttagcc agatggtctc gatctcctga cctcgtgatc
     7681 cacctccctc agcctctcaa agtgctggga ttacaggcat gagccaccgt gcacagccaa
     7741 gatggaaatt tttatgttct ttgtttgata tggtttggct gtgtccccac ccaaatctca
     7801 acttgaactg tatctctcag aattcccaca tgttgtggga gggacccaca gggaggtaat
     7861 tgaatcacaa gggccggttt ttaccatgct ttcttgtgat agtgaataag tctcacaaga
     7921 tctgatgggt ttatcagggg tttccgattt tgcttcttcc tcattttctc ttgccactcc
     7981 catgtaagaa gtgcctttcg cctcccgcca agattctgag gcttcctcag ccatgtggaa
     8041 ctttggggac gaaaagaggt ttaattggac ttaaagttcc aattaaagtt tcaggtatgt
     8101 ctttatcagc agcatgaaaa tgaactaata cagtgtttta tcactttttt tttttttttt
     8161 tttttttgag atggagtttc gctcttgttg cccaggctgg agtgcaatgg cgcaacatca
     8221 ggtcactgca aactctgcct cccgggttca agcgattctc ttgcctcagc ctcctgagta
     8281 gctgggatta caggcttgtg ccaccacacc cggctaattt ttgtattttt agtagagaca
     8341 gagtttcacc atgttggcca ggctagtctt gaactcctga cctcaggtga tccacccacc
     8401 ttggcttccc aaagtgggtg gatgacctct gggattacag gcgtgcgcca ccgcgctcgg
     8461 tctatattca ccaattttaa gatgcacatc tttccatatt ttagtatctc taagttggag
     8521 tatgtcttac aattaatggc atctgacagt tataactggc agcatttgtg actgaaaaac
     8581 atcttagatg caatgaaata cagtgtgtgt gtttgtattg tgctgcaata taaaatgtat
     8641 ttcaggccag gtatggtggt tcacacctgt aatcccagca ctttggaagg ccaaagtggg
     8701 aggatcactt gagcccagga gttcaaggct gcagtgagct ctgattatac cactgtactc
     8761 cagcctaggc aacagagcaa gacccagtct ctaaaaaata aatgaaaatg tttcttattg
     8821 tggatcacag tcaaaagttt gaaaaaacac aatctgtttg aacagcacta ctttctgaca
     8881 ggaaaacccc aacaggttag gatctgtgta ttcctcagag taagaatttt tctaaaaagt
     8941 ttttaaaaac ttaaagtctt actgatctct tcctatgctc caattttata gatataactt
     9001 agccttgcga ggtgatgtaa ttaaagtcta acttcaagtt gctgttgagc atagatctca
     9061 ggtcttctga cttcacaaca cctttctgcc actttcagtg gatttttccc tcctagggac
     9121 cagaggtcct ctactggatt aaaagtttct ccatcccggc agggtgcggt ggctcacgcc
     9181 tgtaatccca acactttggg aggccaaggc gggcggatca cgaggtcagg aaatagagac
     9241 catcctggct aacatggtga aaccccgtgt ctactaaaaa tacgaaaaat tagccgggtg
     9301 tggtggtgcg cgcctgtagt cccagctact cgggaggctg aggcaggaga atggcgtgaa
     9361 gccaggaggc ggagcttgca atgagccgag atcgcgccac tgcactctag cctgggcgac
     9421 aaagcgagac tctgtctcaa aaaaaaaaaa aaaaaaaaaa aattctccat cccttttgaa
     9481 caatctggaa gtctgtttct cttaaccttc agttcccatt ccaatccttt gtaacctatt
     9541 tcaggttaag catttatgga ggtgccatgc ttaattagcc attatggcac tcactctatt
     9601 tcaaaggtta tcccagaatt tggaggctgt aggggaattc agagtgtgaa tttcacttga
     9661 acagcaccct ctgcagactt aaaatattcc tcctatatct gaagacaata ataatttctt
     9721 agaatttgct aggctaaaca actatttttt agtccagtgg cttgtaatta agtcattttt
     9781 agtctttaat tatgttggtt gcttttagaa ttctctttta gagttggtct acatcctttt
     9841 aaaacatggg caatccaaat ttataacagt aaattaagat acataaaaaa aaacactggc
     9901 taaatttaaa aggaaacact tctagaatat actgtatttt gacacaagac cagactgtgc
     9961 tatgtgtatg tggtgtttca agtaatttaa gaaaactgtt ggaattttct gtatttccag
    10021 tttcacaaga aacaacctca aggagggcag tttaactgaa aattcagagg tattatagct
    10081 ctgaagaaaa atactgatga gcagttatac aaaatgagaa attgagttct aagaaatgca
    10141 tccctaactt caacataaag atagctatga gaaaacattc tttgtaccca accataaatg
    10201 aataaaaatc acctcatttc tcatcagatg tttactgggt tgctagttat atatagaatc
    10261 ctgcaagagg ctcaacaggg aagtccaaag agtcaatcaa gaaggtatga taatggctaa
    10321 agatggggac tgtaggtcaa tgctccacga ggttcttctt ttgttgcacc aatatagctg
    10381 cactgttata cccatatggt tacaatccag gcctcagaaa atgttgcaga tgccctcacc
    10441 agaaggctga gaaccagttc ctcagtttgc atgcctccag acagcactag aagtcaaact
    10501 caaattgctt acatggtaac ttggtttatc ccaacaccaa ctaggatatt ccctcaatca
    10561 catgattgta ctgaaaatat aaccaagttt tatttatgtt tttcatttgg tattagccat
    10621 ctggtattta acctctgaaa accacacaat cttctatacg cttttttcac tggaataaat
    10681 gaaactgctt acttttga
//
