LOCUS       NM_005957               7018 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens methylenetetrahydrofolate reductase (MTHFR),
            transcript variant 2, mRNA.
ACCESSION   NM_005957
VERSION     NM_005957.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Ren,F.J., Fang,G.Y. and Zhang,Z.Y.
  TITLE     Association between methylenetetrahydrofolate reductase C677T
            polymorphisms and male oligozoospermia, asthenozoospermia or
            oligoasthenozoospermia: a case-control study
  JOURNAL   Sci Rep 14 (1), 25219 (2024)
   PUBMED   39448729
  REMARK    GeneRIF: Association between methylenetetrahydrofolate reductase
            C677T polymorphisms and male oligozoospermia, asthenozoospermia or
            oligoasthenozoospermia: a case-control study.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Alam,N.F., Ahmed,R., Mahmud,Z., Tamanna,S., Shaon,M.A. and
            Howlader,M.Z.H.
  TITLE     Genetic association and computational analysis of MTHFR gene
            polymorphisms rs1801131 and rs1801133 with breast cancer in the
            Bangladeshi population
  JOURNAL   Sci Rep 14 (1), 24232 (2024)
   PUBMED   39414907
  REMARK    GeneRIF: Genetic association and computational analysis of MTHFR
            gene polymorphisms rs1801131 and rs1801133 with breast cancer in
            the Bangladeshi population.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7018)
  AUTHORS   Zhang,Y., Zhang,Y., Miao,R., Fang,X., Yin,R., Guan,H. and Tian,J.
  TITLE     The MTHFR C677T/A1298C polymorphism is associated with increased
            risk of microangiopathy in type 2 diabetes mellitus: A systematic
            review and meta-analysis
  JOURNAL   Nutr Res 130, 34-47 (2024)
   PUBMED   39340999
  REMARK    GeneRIF: The MTHFR C677T/A1298C polymorphism is associated with
            increased risk of microangiopathy in type 2 diabetes mellitus: A
            systematic review and meta-analysis.
REFERENCE   4  (bases 1 to 7018)
  AUTHORS   Efat,A., Shoeib,S., Abdelmonem,A.A., Elamawy,M.M., Eleleimy,H.A.,
            Ibrahem,R.A., Elmorshedy,S.M. and Abdelaty,M.M.
  TITLE     Clinical characteristics and thrombophilia associated gene variants
            in Egyptians with unprovoked venous thromboembolism: three centers
            experience
  JOURNAL   Mol Biol Rep 51 (1), 1013 (2024)
   PUBMED   39325233
  REMARK    GeneRIF: Clinical characteristics and thrombophilia associated gene
            variants in Egyptians with unprovoked venous thromboembolism: three
            centers experience.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7018)
  AUTHORS   Li,M.H., Chen,I.C., Yang,H.W., Yen,H.C., Ke,Y.Y., Chen,Y.M. and
            Hsu,C.C.
  TITLE     Association of Methylenetetrahydrofolate Reductase rs1801133
            Polymorphism with osteoporosis and fracture risk in Taiwan
  JOURNAL   Int J Med Sci 21 (12), 2261-2271 (2024)
   PUBMED   39310265
  REMARK    GeneRIF: Association of Methylenetetrahydrofolate Reductase
            rs1801133 Polymorphism with osteoporosis and fracture risk in
            Taiwan.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7018)
  AUTHORS   Chen,J., Giovannucci,E., Kelsey,K., Rimm,E.B., Stampfer,M.J.,
            Colditz,G.A., Spiegelman,D., Willett,W.C. and Hunter,D.J.
  TITLE     A methylenetetrahydrofolate reductase polymorphism and the risk of
            colorectal cancer
  JOURNAL   Cancer Res 56 (21), 4862-4864 (1996)
   PUBMED   8895734
REFERENCE   7  (bases 1 to 7018)
  AUTHORS   Goyette,P., Frosst,P., Rosenblatt,D.S. and Rozen,R.
  TITLE     Seven novel mutations in the methylenetetrahydrofolate reductase
            gene and genotype/phenotype correlations in severe
            methylenetetrahydrofolate reductase deficiency
  JOURNAL   Am J Hum Genet 56 (5), 1052-1059 (1995)
   PUBMED   7726158
REFERENCE   8  (bases 1 to 7018)
  AUTHORS   Frosst,P., Blom,H.J., Milos,R., Goyette,P., Sheppard,C.A.,
            Matthews,R.G., Boers,G.J., den Heijer,M., Kluijtmans,L.A., van den
            Heuvel,L.P. et al.
  TITLE     A candidate genetic risk factor for vascular disease: a common
            mutation in methylenetetrahydrofolate reductase
  JOURNAL   Nat Genet 10 (1), 111-113 (1995)
   PUBMED   7647779
REFERENCE   9  (bases 1 to 7018)
  AUTHORS   Goyette,P., Sumner,J.S., Milos,R., Duncan,A.M., Rosenblatt,D.S.,
            Matthews,R.G. and Rozen,R.
  TITLE     Human methylenetetrahydrofolate reductase: isolation of cDNA
            mapping and mutation identification
  JOURNAL   Nat Genet 7 (4), 551 (1994)
   PUBMED   7951330
  REMARK    Correction to:[Nat Genet. 1994 Jun;7(2):195-200. doi:
            10.1038/ng0694-195.. PMID: 7920641]
REFERENCE   10 (bases 1 to 7018)
  AUTHORS   Goyette,P., Sumner,J.S., Milos,R., Duncan,A.M., Rosenblatt,D.S.,
            Matthews,R.G. and Rozen,R.
  TITLE     Human methylenetetrahydrofolate reductase: isolation of cDNA,
            mapping and mutation identification
  JOURNAL   Nat Genet 7 (2), 195-200 (1994)
   PUBMED   7920641
  REMARK    Erratum:[Nat Genet. 1994 Aug;7(4):551.. PMID: 7951330]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY046560.1, AB209113.1,
            AL953897.6, AJ237672.1 and BC053509.1.
            
            On Aug 31, 2019 this sequence version replaced NM_005957.4.
            
            Summary: The protein encoded by this gene catalyzes the conversion
            of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a
            co-substrate for homocysteine remethylation to methionine. Genetic
            variation in this gene influences susceptibility to occlusive
            vascular disease, neural tube defects, colon cancer and acute
            leukemia, and mutations in this gene are associated with
            methylenetetrahydrofolate reductase deficiency.[provided by RefSeq,
            Oct 2009].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (2) is
            shorter at the N-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.2408336.1, BC053509.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376590.9/ ENSP00000365775.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-179               AY046560.1         957-1135
            180-1375            AB209113.1         357-1552
            1376-1395           AL953897.6         80424-80443         c
            1396-2274           AJ237672.1         1435-2313
            2275-3350           BC053509.1         2236-3311
            3351-3919           AL953897.6         74846-75414         c
            3920-4729           BC053509.1         3881-4690
            4730-6138           AB209113.1         3669-5077
            6139-6813           AL953897.6         71952-72626         c
            6814-7011           AB209113.1         5756-5953
            7012-7018           AL953897.6         71747-71753         c
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.22"
     gene            1..7018
                     /gene="MTHFR"
                     /note="methylenetetrahydrofolate reductase"
                     /db_xref="GeneID:4524"
                     /db_xref="HGNC:HGNC:7436"
                     /db_xref="MIM:607093"
     exon            1..77
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            78..326
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..2061
                     /gene="MTHFR"
                     /EC_number="1.5.1.53"
                     /note="isoform 2 is encoded by transcript variant 2;
                     5,10-methylenetetrahydrofolate reductase (NADPH);
                     methylenetetrahydrofolate reductase (NAD(P)H)"
                     /codon_start=1
                     /product="methylenetetrahydrofolate reductase (NADPH)
                     isoform 2"
                     /protein_id="NP_005948.3"
                     /db_xref="CCDS:CCDS137.1"
                     /db_xref="GeneID:4524"
                     /db_xref="HGNC:HGNC:7436"
                     /db_xref="MIM:607093"
                     /translation="MVNEARGNSSLNPCLEGSASSGSESSKDSSRCSTPGLDPERHER
                     LREKMRRRLESGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPG
                     SDKETSSMMIASTAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDP
                     IGDQWEEEEGGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEAGSFEADLKHLKEKV
                     SAGADFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEV
                     PQEIKDVIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLK
                     RLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNS
                     SSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKV
                     TCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVF
                     QKAYLEFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPG
                     REIIQPTVVDPVSFMFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDN
                     DFPLDNCLWQVVEDTLELLNRPTQNARETEAP"
     exon            327..565
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            566..676
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            677..870
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            871..1121
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1256
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            1257..1437
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1620
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1722
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1842
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..7018
                     /gene="MTHFR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6996..7001
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTHFR"
                     /note="hexamer: AACAAA"
     polyA_site      7018
                     /gene="MTHFR"
                     /note="major polyA site"
ORIGIN      
        1 actgcgttcc ccgcccctgc agcggccaca gtggtgcggc cggcggccga gcgttctgag
       61 tcacccggga ctggaggtag gaacccagcc atggtgaacg aagccagagg aaacagcagc
      121 ctcaacccct gcttggaggg cagtgccagc agtggcagtg agagctccaa agatagttcg
      181 agatgttcca ccccgggcct ggaccccgag cggcatgaga gactccggga gaagatgagg
      241 cggcgattgg aatctggtga caagtggttc tccctggaat tcttccctcc tcgaactgct
      301 gagggagctg tcaatctcat ctcaaggttt gaccggatgg cagcaggtgg ccccctctac
      361 atagacgtga cctggcaccc agcaggtgac cctggctcag acaaggagac ctcctccatg
      421 atgatcgcca gcaccgccgt gaactactgt ggcctggaga ccatcctgca catgacctgc
      481 tgccgtcagc gcctggagga gatcacgggc catctgcaca aagctaagca gctgggcctg
      541 aagaacatca tggcgctgcg gggagaccca ataggtgacc agtgggaaga ggaggaggga
      601 ggcttcaact acgcagtgga cctggtgaag cacatccgaa gtgagtttgg tgactacttt
      661 gacatctgtg tggcaggtta ccccaaaggc caccccgaag cagggagctt tgaggctgac
      721 ctgaagcact tgaaggagaa ggtgtctgcg ggagccgatt tcatcatcac gcagcttttc
      781 tttgaggctg acacattctt ccgctttgtg aaggcatgca ccgacatggg catcacttgc
      841 cccatcgtcc ccgggatctt tcccatccag ggctaccact cccttcggca gcttgtgaag
      901 ctgtccaagc tggaggtgcc acaggagatc aaggacgtga ttgagccaat caaagacaac
      961 gatgctgcca tccgcaacta tggcatcgag ctggccgtga gcctgtgcca ggagcttctg
     1021 gccagtggct tggtgccagg cctccacttc tacaccctca accgcgagat ggctaccaca
     1081 gaggtgctga agcgcctggg gatgtggact gaggacccca ggcgtcccct accctgggct
     1141 ctcagcgccc accccaagcg ccgagaggaa gatgtacgtc ccatcttctg ggcctccaga
     1201 ccaaagagtt acatctaccg tacccaggag tgggacgagt tccctaacgg ccgctggggc
     1261 aattcctctt cccctgcctt tggggagctg aaggactact acctcttcta cctgaagagc
     1321 aagtccccca aggaggagct gctgaagatg tggggggagg agctgaccag tgaagaaagt
     1381 gtctttgaag tcttcgttct ttacctctcg ggagaaccaa accggaatgg tcacaaagtg
     1441 acttgcctgc cctggaacga tgagcccctg gcggctgaga ccagcctgct gaaggaggag
     1501 ctgctgcggg tgaaccgcca gggcatcctc accatcaact cacagcccaa catcaacggg
     1561 aagccgtcct ccgaccccat cgtgggctgg ggccccagcg ggggctatgt cttccagaag
     1621 gcctacttag agtttttcac ttcccgcgag acagcggaag cacttctgca agtgctgaag
     1681 aagtacgagc tccgggttaa ttaccacctt gtcaatgtga agggtgaaaa catcaccaat
     1741 gcccctgaac tgcagccgaa tgctgtcact tggggcatct tccctgggcg agagatcatc
     1801 cagcccaccg tagtggatcc cgtcagcttc atgttctgga aggacgaggc ctttgccctg
     1861 tggattgagc ggtggggaaa gctgtatgag gaggagtccc cgtcccgcac catcatccag
     1921 tacatccacg acaactactt cctggtcaac ctggtggaca atgacttccc actggacaac
     1981 tgcctctggc aggtggtgga agacacattg gagcttctca acaggcccac ccagaatgcg
     2041 agagaaacgg aggctccatg accctgcgtc ctgacgccct gcgttggagc cactcctgtc
     2101 ccgccttcct cctccacagt gctgcttctc ttgggaactc cactctcctt cgtgtctctc
     2161 ccaccccggc ctccactccc ccacctgaca atggcagcta gactggagtg aggcttccag
     2221 gctcttcctg gacctgagtc ggccccacat gggaacctag tactctctgc tctagccagg
     2281 agtctgtgct cttttggtgg ggagcacttg cgtcctgcag aggaccacag tgggtggcac
     2341 ctcctgagaa ggcgaggaga gtggttgttg ccaactaagc cctcgaacca aggcagcctc
     2401 cagagccagc ctgggactcc cagtgaactt acacttggag cccgtgcagt acaggcaaaa
     2461 cacgcaaggg catcaggcac tggtggcatc gtagaagaga tgtggcaaag tgctgtaccc
     2521 ttccacctcc tagaggtggg cagctgggcc ccacctactt gtgactgaag gggcacacca
     2581 ctgccctgcc tgcccactta gccgtccatg gcaccagccc cctggatggg cattgggctg
     2641 acacctacca tgctgctttt tggcacagtt gtctattctg agccttgaga gaaaaagtgc
     2701 cccttaaggg ttgaaggcag tctgaaccct tgtgcttggt ggggctcgtg gccttcccct
     2761 tttgcctggc tgtggaggcc tgatgctgcc ccgttccctg tcagaggcta agatgagatt
     2821 tgccagcaca ggggccccag atctgcctgg gcctgtgcag cagcccagct tcctggtgta
     2881 tttttcaggt aggcccttgt cctgccagct gccttcctca tcccctcgtc ctgtcccaga
     2941 ggttatctgc ctggcctggc tccccacgag tcacctgcaa gccccagggc ctgggggcag
     3001 tgactggcag gtgcagatgg gctgtttcgt gtagtggaag agcagcctga tggccaaggg
     3061 ggtggacgca attgtgggat gtcctcttta ctcccttcct ggcctcactg gctggggcag
     3121 aggggcagcc gctaggagag actgaaagca gcagctagga ctgaggagtg ggttttattg
     3181 tccttcagag ctcttcaagc tgtcccctct gtcatcactc cctggatgtg tggggcatgg
     3241 ttccttccct gggaaggcta agttcagttc tgttttttat tctatgagaa caagtcacag
     3301 ctgcagctgg gccccatgct ctgccccaag cccccaaccc cgcggtgctc tggcggcttc
     3361 ctgtccactc tcggggccct tggggcctgg cttgctccag ggtcttgggc tactggcagc
     3421 tcctctcctt gggctcctgg ctgccaggcg ttggtgccac ttcttaaagg cctggaacca
     3481 gggaggagag gaaatgctat tgttgtgggc tttctccggg gtctgtgctg tgcctgctag
     3541 agcaacccct gtacccagct ccttttgtcc ccagggcccc tccctctgcc ccaagcagcc
     3601 agccagtctt gcctaggcca aatgcacaag ctcagaatag atctgatggt gagctgggaa
     3661 gctgtactca gagcagagca aatgagggag ggggcgctca ggacccaggc cctccatggg
     3721 ctagtgtgag tggcagccat gcctcatgcc acaccttctt cgcaaactga tggaccgggt
     3781 gggcctggcc tgagctgggg ccacaaatca aagcaagggc tccagcatcc agcctgtgtg
     3841 ttctgtaatg gaactgaccc cctcccctga aaacgaaggg gccccggggc tggcaagcag
     3901 ggaaagctcc acggtgcgtg gctgtggcac agacttctgg aaggctggct gagtggaatg
     3961 cagggaagag ggcagtacct gggaaaggac ccacccatct tcctgctgct gtaactgctg
     4021 agccactcgc agtcgcagga tccgctgcca ccacgtctgc caggcccatc tcaggtgcca
     4081 ctccctgagc tttggggaca gttggcagag aaggcctctt gtgctcacgc tcccccgcag
     4141 tccccagccc ttctgccttt ctcccccgac actgctgcac cagagtgaaa gggctatggc
     4201 aagggggtgt catctgagga gtattaagaa tgcagattcc tgggcctgtc ccccaaggtt
     4261 ttggagtcag taggtccaag ggccatactt ttgagagggg tttgggttaa gtatgaggtg
     4321 aaatgggaga tggtcagtgt ggagaggggt gcacccactc accagggtcc gcaccagctg
     4381 ctctgcccct tgggcatcca cccagtgctg ccatgccact gccaggcacc tggcctgctg
     4441 ggaaccccgc agcccgtgaa gcagtgcctc gaggcaccgg cgctgcaggt acttcctcct
     4501 gatggccaag agcatcgtga cccttcaggg ccagaaggag ggcagagcca tgggcctggg
     4561 cctgcttttc caggatcctg caggaacgag cactggccag agagggccca gctgtagcca
     4621 tggctcaggc aagcccctca gcccttgccc ccatccctcg gacccaccaa actgcacaca
     4681 cagctcctct taccgtagcc tccgtttatg ggccttgctt tgggctttgc aggctctggg
     4741 ctcagggctg gagtgcgctc ttggtccctg gtccctcgtc cacaggggca ggcctgggac
     4801 ccagctactc tgtccaggcc actgtggcca gagctggaag gcagggcaga gggaatgttc
     4861 cctgcaccct ggaaagggga gttgagtcac aagaggttaa ggtgggtcca ggaaggcagc
     4921 tgctcttagt gcccgcctag gagttgagta cagtgaggag ggtggaggaa ggtgctgagc
     4981 ttagccttgt gccctgcccc catctcccca ggcctccagc ctctcccggc tgcctgccgc
     5041 ccaaagagaa atcacagggg cggggcagga atgcaaagtg ttttctcaga acagctgaaa
     5101 cattccgaag agggaatgga tggggagaat ggtcaataca cataagaccg tgtcccaagg
     5161 agctgatttc caggcccctg aggactggag accgcttcac ccctgcactt cagacaccgt
     5221 ttgtcccccg gggcaaggtc tccttactct gagcccaggc cgttcccctt ggcttcctcc
     5281 gtccacccag gctgcactgc agtgatggcg cgggaggcac cagctctgtg gcctgtgtcc
     5341 agcagctgcg ggtctgaagg aatagccaga gaggagcacc tgaaccccat gggcttggac
     5401 ttcctggggc cccgctggga tttcttcgct gctctagctg gcaggacaca tcccggcctc
     5461 ttccacccat tcccccatgt ggctgaagac attccaacaa tggggtgggc ccataatagt
     5521 tagccctcag tcagttcccg gagcacagcc ctgggagggg gctatttctc tccccactga
     5581 aaacatttca aagctgagtt acttgtctga ggcctcatcc ctcggaagcc gtctgactcc
     5641 agagtctgag cccccggcta gtaccctata gagagggggc tctccaaagg ggctgctggg
     5701 gcatgtgtgc ctgtggcaga aaagaggaga ccctggaatt cagcaccctg ggtgccattc
     5761 ccagcgttta gtttctagag gcctcagttt ctccatcagc ttatgggatc cttgtcttta
     5821 ctgacaagaa tggaatagaa atgtaaaagt actctgaaaa gcaattgccc tgtaacttat
     5881 ctagaaagaa aagaccctga gactccagaa tctgctgttg ccatagcccc atatgtgtga
     5941 attctgcaac tagccaaggc tagttccttt caattccatt taaaaaacaa aaaccagcag
     6001 gtgtggtggc tcatggcgta atgggcctgc ccaatgcttt gggaggccaa ggcaggtaga
     6061 tcgcttgagc ccaggagttt gagacaagcc ctggcaacat agtgagatcc catttctaca
     6121 aaaaaaaaaa aaaaaaaatt agccgggtgt ggtggcacac gcctgtagtc tcagctactc
     6181 aggtggctga ggtgggagga tcgtttgagc cctggaggtt gaggctgcag tgagctgtga
     6241 tcgcaccact gcactctggc ctgggcgaca gagtgagaca ctgtctgaga acaaaaaacg
     6301 actgaaaaaa aaaatcacct tagctttttc tcttagaatc ttctctaaaa cgtattcttt
     6361 gtggcattct gaaataggat tcatgatgat gcctgttgat cttagggaca ctacctcacc
     6421 tgccagtatc tttggggctg tgtccttcaa ggacatgtcc ccagactgct gtgcagtgtc
     6481 attttttgtg tttggtttgg tggtggcttc ttcccccttg ctaggctatc aacctcttat
     6541 caccacttgt tggtgtcaga actaactgct tctggtctgg agagggactg accgatgcct
     6601 ttgggtagag agaattatga aagaaatttt ggtatttttc tactttatat tttctgaggt
     6661 ttctgtaata agcatatttc acttttccaa taagaaaaaa aaaaacttgg cctggcgcgg
     6721 tggctcacac ctgtaatccc agcactttgg gaagtcgagg tgggaggatc acttgagttc
     6781 aggagttcga gaccagcttg ggcaatatgg tgaaaccccg tctctactaa aaatacaaaa
     6841 attagccagg cgtggtggcg tgcacttgta gtcccagcta ctcaggaagc tgaggcggga
     6901 gaatcacttg aacccgggag gcagaggttg cagtgagctg agatcactcc tctgcttcag
     6961 cctgggcaat tgagccagac tctgtctcaa aaataaacaa aaaaacttga caaaaaaa
//
