LOCUS       NM_001350493            3339 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+
            dependent) 1 like (MTHFD1L), transcript variant 12, mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_001350493
VERSION     NM_001350493.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Chen,Z.W., Hu,J.F., Wang,Z.W., Liao,C.Y., Kang,F.P., Lin,C.F.,
            Huang,Y., Huang,L., Tian,Y.F. and Chen,S.
  TITLE     Circular RNA circ-MTHFD1L induces HR repair to promote gemcitabine
            resistance via the miR-615-3p/RPN6 axis in pancreatic ductal
            adenocarcinoma
  JOURNAL   J Exp Clin Cancer Res 41 (1), 153 (2022)
   PUBMED   35459186
  REMARK    GeneRIF: Circular RNA circ-MTHFD1L induces HR repair to promote
            gemcitabine resistance via the miR-615-3p/RPN6 axis in pancreatic
            ductal adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Sial,N., Rehman,J.U., Saeed,S., Ahmad,M., Hameed,Y., Atif,M.,
            Rehman,A., Asif,R., Ahmed,H., Hussain,M.S., Khan,M.R., Ambreen,A.
            and Ambreen,A.
  TITLE     Integrative analysis reveals methylenetetrahydrofolate
            dehydrogenase 1-like as an independent shared diagnostic and
            prognostic biomarker in five different human cancers
  JOURNAL   Biosci Rep 42 (1) (2022)
   PUBMED   34908119
  REMARK    GeneRIF: Integrative analysis reveals methylenetetrahydrofolate
            dehydrogenase 1-like as an independent shared diagnostic and
            prognostic biomarker in five different human cancers.
REFERENCE   3  (bases 1 to 3339)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   4  (bases 1 to 3339)
  AUTHORS   Wang,L., Yang,Y., Wang,X.M., Wang,C.Q., Zhang,Y.M. and Li,B.L.
  TITLE     MTHFD1L as a folate cycle enzyme correlates with prognostic outcome
            and its knockdown impairs cell invasive behaviors in osteosarcoma
            via mediating the AKT/mTOR pathway
  JOURNAL   J Recept Signal Transduct Res 40 (6), 584-590 (2020)
   PUBMED   32456526
  REMARK    GeneRIF: MTHFD1L as a folate cycle enzyme correlates with
            prognostic outcome and its knockdown impairs cell invasive
            behaviors in osteosarcoma via mediating the AKT/mTOR pathway.
REFERENCE   5  (bases 1 to 3339)
  AUTHORS   Ali,M., Lemonakis,K., Wihlborg,A.K., Veskovski,L., Turesson,I.,
            Mellqvist,U.H., Gullberg,U., Hansson,M. and Nilsson,B.
  TITLE     Sequence variation at the MTHFD1L-AKAP12 and FOPNL loci does not
            influence multiple myeloma survival in Sweden
  JOURNAL   Blood Cancer J 9 (8), 57 (2019)
   PUBMED   31363079
  REMARK    GeneRIF: Results together with the limitations of the original
            studies indicate that the reported associations between the MTHFD1L
            and FOPNL loci and MM survival are false positives due to a
            winner's curse effect.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3339)
  AUTHORS   Walkup,A.S. and Appling,D.R.
  TITLE     Enzymatic characterization of human mitochondrial
            C1-tetrahydrofolate synthase
  JOURNAL   Arch Biochem Biophys 442 (2), 196-205 (2005)
   PUBMED   16171773
  REMARK    GeneRIF: Gene encodes the mitochondrial isozyme of
            C1-tetrahydrofolate (THF) synthase, a monofunctional enzyme
            containing formyl-THF synthetase activity.
REFERENCE   7  (bases 1 to 3339)
  AUTHORS   Christensen,K.E., Patel,H., Kuzmanov,U., Mejia,N.R. and
            MacKenzie,R.E.
  TITLE     Disruption of the mthfd1 gene reveals a monofunctional
            10-formyltetrahydrofolate synthetase in mammalian mitochondria
  JOURNAL   J Biol Chem 280 (9), 7597-7602 (2005)
   PUBMED   15611115
REFERENCE   8  (bases 1 to 3339)
  AUTHORS   Sugiura,T., Nagano,Y., Inoue,T. and Hirotani,K.
  TITLE     A novel mitochondrial C1-tetrahydrofolate synthetase is upregulated
            in human colon adenocarcinoma
  JOURNAL   Biochem Biophys Res Commun 315 (1), 204-211 (2004)
   PUBMED   15013446
REFERENCE   9  (bases 1 to 3339)
  AUTHORS   Prasannan,P., Pike,S., Peng,K., Shane,B. and Appling,D.R.
  TITLE     Human mitochondrial C1-tetrahydrofolate synthase: gene structure,
            tissue distribution of the mRNA, and immunolocalization in Chinese
            hamster ovary calls
  JOURNAL   J Biol Chem 278 (44), 43178-43187 (2003)
   PUBMED   12937168
  REMARK    GeneRIF: mitochondrial C1-tetrahydrofolate synthase gene structure
            and tissue distribution
REFERENCE   10 (bases 1 to 3339)
  AUTHORS   Fountoulakis,M., Gulesserian,T. and Lubec,G.
  TITLE     Overexpression of C1-tetrahydrofolate synthase in fetal Down
            syndrome brain
  JOURNAL   J Neural Transm Suppl (67), 85-93 (2003)
   PUBMED   15068241
  REMARK    GeneRIF: Overexpression of C1-tetrahydrofolate synthase in fetal
            Down syndrome brain at the early second trimester may indicate
            abnormal folate metabolism and may reflect folate deficiency.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL035086.12, AL049694.9 and
            AL133260.12.
            
            Summary: The protein encoded by this gene is involved in the
            synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is
            important in the de novo synthesis of purines and thymidylate and
            in the regeneration of methionine from homocysteine. Several
            transcript variants encoding different isoforms have been found for
            this gene.[provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (12), as well as variant 11,
            encodes isoform 11.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.95886.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2146411, SAMEA2146982
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-233               AL035086.12        57736-57968         c
            234-318             AL035086.12        48241-48325         c
            319-369             AL035086.12        46731-46781         c
            370-423             AL035086.12        46589-46642         c
            424-548             AL035086.12        41529-41653         c
            549-649             AL035086.12        38681-38781         c
            650-789             AL035086.12        36434-36573         c
            790-901             AL035086.12        18654-18765         c
            902-993             AL035086.12        5747-5838           c
            994-1091            AL035086.12        2113-2210           c
            1092-1265           AL049694.9         98203-98376         c
            1266-1402           AL049694.9         87558-87694         c
            1403-1449           AL049694.9         85753-85799         c
            1450-1557           AL049694.9         79905-80012         c
            1558-1632           AL049694.9         78957-79031         c
            1633-1735           AL049694.9         75365-75467         c
            1736-1812           AL049694.9         68427-68503         c
            1813-1953           AL049694.9         64083-64223         c
            1954-2022           AL049694.9         59461-59529         c
            2023-2134           AL049694.9         52440-52551         c
            2135-2274           AL049694.9         14540-14679         c
            2275-2316           AL049694.9         10678-10719         c
            2317-2417           AL049694.9         9518-9618           c
            2418-2595           AL049694.9         8805-8982           c
            2596-2703           AL133260.12        99104-99211         c
            2704-2856           AL133260.12        96587-96739         c
            2857-2977           AL133260.12        41117-41237         c
            2978-3339           AL133260.12        31817-32178         c
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q25.1"
     gene            1..3339
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /note="methylenetetrahydrofolate dehydrogenase (NADP+
                     dependent) 1 like"
                     /db_xref="GeneID:25902"
                     /db_xref="HGNC:HGNC:21055"
                     /db_xref="MIM:611427"
     exon            1..233
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            234..318
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            319..369
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     CDS             337..2946
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /EC_number="6.3.4.3"
                     /note="isoform 11 is encoded by transcript variant 12;
                     10-formyl-THF synthetase; formyltetrahydrofolate
                     synthetase domain containing 1; monofunctional
                     C1-tetrahydrofolate synthase, mitochondrial"
                     /codon_start=1
                     /product="monofunctional C1-tetrahydrofolate synthase,
                     mitochondrial isoform 11"
                     /protein_id="NP_001337422.1"
                     /db_xref="GeneID:25902"
                     /db_xref="HGNC:HGNC:21055"
                     /db_xref="MIM:611427"
                     /translation="MQEINQNLAEEAGLNITHICLPPDSSEAEIIDEILKINEDTRVH
                     GLALQISENLFSNKVLNALKPEKDVDGVTDINLGKLVRGDAHECFVSPVAKAVIELLE
                     KSVGVNLDGKKILVVGAHGSLEAALQCLFQRKGSMTMSIQWKTRQLQSKLHEADIVVL
                     GSPKPEEIPLTWIQPGTTVLNCSHDFLSGKVGCGSPRIHFGGLIEEDDVILLAAALRI
                     QNMVSSGRRWLREQQHRRWRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLA
                     DEIEIYGKSKAKVRLSVLERLKDQADGKYVLVAGITPTPLGEGKSTVTIGLVQALTAH
                     LNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDIHAITAANNLLAA
                     AIDTRILHENTQTDKALYNRLVPLVNGVREFSEIQLARLKKLGINKTDPSTLTEEEVS
                     KFARLDIDPSTITWQRVLDTNDRFLRKITIGQGNTEKGHYRQAQFDIAVASEIMAVLA
                     LTDSLADMKARLGRMVVASDKSGQPVTADDLGVTGALTVLMKDAIKPNLMQTLEGTPV
                     FVHAGPFANIAHGNSSVLADKIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGL
                     VPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEENIQLVADGCCNLQKQIQITQLF
                     GVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAA
                     SKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQGFGNLPICM
                     AKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDI
                     DLDTETEQVKGLF"
     exon            370..423
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            424..548
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            549..649
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            650..789
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            790..901
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            902..993
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1091
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1265
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1402
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1449
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..1557
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1632
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1633..1735
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1812
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..1953
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1954..2022
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2134
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..2274
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2316
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2417
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2418..2595
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2703
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2704..2856
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..2977
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..3339
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3315..3320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /note="hexamer: AATAAA"
     polyA_site      3339
                     /gene="MTHFD1L"
                     /gene_synonym="dJ292B18.2; FTHFSDC1; MTC1THFS"
                     /note="major polyA site"
ORIGIN      
        1 gcggggctgc ggctcggcgc gccggctggc ccggggttcg ggaagggcaa gcggagctcg
       61 ggagaggcgg gctcgggccc agcgccgccc gcgcgaagct ccctggtgtt gtgcgccctt
      121 ccccgcgcgc ggcccctcct gctgggctgg ggtctgtggc tgacgtccgc ttttcccgga
      181 acggcaaagg tggagccggg cccccgggac agccgccggg gggaatccga gagagaagtc
      241 attcagaatt caaaagaagt tctaagttta ttgcaagaaa aaaaccctgc cttcaagccg
      301 gttcttgcaa ttatccaggc aggtgacgac aacttgatgc aggaaatcaa ccagaatttg
      361 gctgaggagg ctggtctgaa catcactcac atttgcctcc ctccagatag cagtgaagcc
      421 gagattatag atgaaatctt aaagatcaat gaagatacca gagtacatgg ccttgccctt
      481 cagatctctg agaacttgtt tagcaacaaa gtcctcaatg ccttgaaacc agaaaaagat
      541 gtggatggag taacagacat aaacctgggg aagctggtgc gaggggatgc ccatgaatgt
      601 tttgtttcac ctgttgccaa agctgtaatt gaacttcttg aaaaatcagt aggtgtcaac
      661 ctagatggaa agaagatttt ggtagtgggg gcccatgggt ctttggaagc tgctctacaa
      721 tgcctgttcc agagaaaagg gtccatgaca atgagcatcc agtggaaaac acgccagctt
      781 caaagcaagc ttcacgaggc tgacattgtg gtcctaggct cacctaagcc agaagagatt
      841 ccccttactt ggatacaacc aggaactact gttctcaact gctcccatga cttcctgtca
      901 gggaaggttg ggtgtggctc tccaagaata cattttggtg gactcattga ggaagatgat
      961 gtgattctcc ttgctgcagc tctgcgaatt cagaacatgg tcagtagtgg aaggagatgg
     1021 cttcgtgaac agcagcacag gcggtggaga cttcactgct tgaaacttca gcctctctcc
     1081 cctgtgccaa gtgacattga gatttcaaga ggacaaactc caaaagctgt ggatgtcctt
     1141 gccaaggaga ttggattgct tgcagatgaa attgaaatct atggcaaaag caaagccaaa
     1201 gtacgtttgt ccgtgctaga aaggttaaag gatcaagcag atggaaaata cgtcttagtt
     1261 gctgggatca cacccacccc tcttggagaa gggaagagca cagtcaccat cgggcttgtg
     1321 caggctctga ccgcacacct gaatgtcaac tcctttgcct gcttgaggca gccttcccaa
     1381 ggaccgacgt ttggagtgaa aggaggagcc gcgggtggtg gatatgccca ggtcatcccc
     1441 atggaggagt tcaaccttca cttgactgga gacatccacg ccatcaccgc tgccaataac
     1501 ttgctggctg ccgccatcga cacgaggatt cttcatgaaa acacgcaaac agataaggct
     1561 ctgtataatc ggctggttcc tttagtgaat ggtgtcagag aattttcaga aattcagctt
     1621 gctcggctaa aaaaactggg aataaataag actgatccga gcacactgac agaagaggaa
     1681 gtgagtaaat ttgcccgtct cgacatcgac ccatctacca tcacgtggca gagagtattg
     1741 gatacaaatg accgatttct acgaaaaata accatcgggc agggaaacac agagaagggc
     1801 cattaccggc aggcgcagtt tgacatcgca gtggccagcg agatcatggc ggtgctggcc
     1861 ctgacggaca gcctcgcaga catgaaggca cggctgggaa ggatggtggt ggccagtgac
     1921 aaaagcgggc agcctgtgac agcagatgat ttgggggtga caggtgcttt gacagttttg
     1981 atgaaagatg caataaaacc aaacctgatg cagaccctgg aagggacacc tgtgttcgtg
     2041 catgcgggcc cttttgctaa cattgctcac ggcaactctt cagtgttggc tgataaaatt
     2101 gccctgaaac tggttggtga agaaggattt gtagtgaccg aagctggctt tggtgctgac
     2161 atcggaatgg agaaattctt caacatcaag tgccgagctt ccggcttggt gcccaacgtg
     2221 gttgtgttag tggcaacggt gcgagctctg aagatgcatg gaggcgggcc aagtgtaacg
     2281 gctggtgttc ctcttaagaa agaatataca gaggagaaca tccagctggt ggcagacggc
     2341 tgctgtaacc tccagaagca aattcagatc actcagctct ttggggttcc cgttgtggtg
     2401 gctctgaatg tcttcaagac cgacacccgc gctgagattg acttggtgtg tgagcttgca
     2461 aagcgggctg gtgcctttga tgcagtcccc tgctatcact ggtccgttgg tggaaaagga
     2521 tcggtggact tggctcgggc tgtgagagag gctgcgagta aaagaagccg attccagttc
     2581 ctgtatgatg ttcaggttcc aattgtggac aagataagga ccattgctca ggctgtctat
     2641 ggagccaaag atattgaact ctctcctgag gcacaagcca aaatagatcg ttacactcaa
     2701 cagggttttg gaaatttgcc catctgcatg gcaaagaccc acctttctct atctcaccaa
     2761 cctgacaaaa aaggtgtgcc aagggacttc atcttaccta tcagtgacgt ccgggccagc
     2821 ataggcgctg ggttcattta ccctttggtc ggaacgatga gcaccatgcc aggactgccc
     2881 acccggccct gcttttatga catagatctt gataccgaaa cagaacaagt taaaggcttg
     2941 ttctaagtgg acaaggctct cacaggaccc gatgcagact cctgaaacag actactcttt
     3001 gcctttttgc tgcagttgga gaagaaactg aatttgaaaa atgtctgtta tgcaatgctg
     3061 gagacatggt gaaataggcc aaagatttct tcttcgttca agatgaattc tgttcacagt
     3121 ggagtatggt gttcggcaaa aggacctcca ccaagactga aagaaactaa tttatttctg
     3181 tttctgtgga gtttccatta tttctactgc ttacacttta gaatgtttat tttatgggga
     3241 ctaagggatt aggagtgtga actaaaaggt aacattttcc actctcaagt tttctacttt
     3301 gtctttgaac tgaaaataaa catggatcta gaaaaccaa
//
