LOCUS       NM_080627              14218 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens microtubule crosslinking factor 2 (MTCL2), transcript
            variant 1, mRNA.
ACCESSION   NM_080627
VERSION     NM_080627.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Li,J., Yang,F., Wang,Z., Zheng,S., Zhang,S., Wang,C., He,B.,
            Wang,J.B. and Wang,H.
  TITLE     METTL16-mediated N6-methyladenosine modification of Soga1 enables
            proper chromosome segregation and chromosomal stability in
            colorectal cancer
  JOURNAL   Cell Prolif 57 (5), e13590 (2024)
   PUBMED   38084791
  REMARK    GeneRIF: METTL16-mediated N6-methyladenosine modification of Soga1
            enables proper chromosome segregation and chromosomal stability in
            colorectal cancer.
REFERENCE   2  (bases 1 to 14218)
  AUTHORS   Matsuoka,R., Miki,M., Mizuno,S., Ito,Y., Yamada,C. and Suzuki,A.
  TITLE     MTCL2 promotes asymmetric microtubule organization by crosslinking
            microtubules on the Golgi membrane
  JOURNAL   J Cell Sci 135 (11) (2022)
   PUBMED   35543016
REFERENCE   3  (bases 1 to 14218)
  AUTHORS   Ferreira,L.T., Logarinho,E., Macedo,J.C., Maia,A.R.R. and Maiato,H.
  TITLE     SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for
            faithful chromosome segregation in human cells
  JOURNAL   Chromosome Res 29 (2), 159-173 (2021)
   PUBMED   33587225
  REMARK    GeneRIF: SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins
            required for faithful chromosome segregation in human cells.
REFERENCE   4  (bases 1 to 14218)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 14218)
  AUTHORS   Habieb,A., Matboli,M., El-Tayeb,H. and El-Asmar,F.
  TITLE     Potential role of lncRNA-TSIX, miR-548-a-3p, and SOGA1 mRNA in the
            diagnosis of hepatocellular carcinoma
  JOURNAL   Mol Biol Rep 46 (4), 4581-4590 (2019)
   PUBMED   31004302
  REMARK    GeneRIF: Authors selected an HCC-specific RNA-based biomarker panel
            that depended on the integration of suppressor of glucose
            autophagy-associated (SOGA1) gene expression with the chosen panel
            of epigenetic regulators of this gene [long non-coding RNA
            antisense for X-inactive-specific transcript (lncRNA-TSIX) and
            microRNA-548-a-3p (miR-548-a-3p)].
            Erratum:[Mol Biol Rep. 2019 Aug;46(4):4591. doi:
            10.1007/s11033-019-04838-z.. PMID: 31049832]
REFERENCE   6  (bases 1 to 14218)
  AUTHORS   Kruse,R., Krantz,J., Barker,N., Coletta,R.L., Rafikov,R., Luo,M.,
            Hojlund,K., Mandarino,L.J. and Langlais,P.R.
  TITLE     Characterization of the CLASP2 Protein Interaction Network
            Identifies SOGA1 as a Microtubule-Associated Protein
  JOURNAL   Mol Cell Proteomics 16 (10), 1718-1735 (2017)
   PUBMED   28550165
REFERENCE   7  (bases 1 to 14218)
  AUTHORS   Cowherd,R.B., Asmar,M.M., Alderman,J.M., Alderman,E.A.,
            Garland,A.L., Busby,W.H., Bodnar,W.M., Rusyn,I., Medoff,B.D.,
            Tisch,R., Mayer-Davis,E., Swenberg,J.A., Zeisel,S.H. and Combs,T.P.
  TITLE     Adiponectin lowers glucose production by increasing SOGA
  JOURNAL   Am J Pathol 177 (4), 1936-1945 (2010)
   PUBMED   20813965
  REMARK    Erratum:[Am J Pathol. 2011 Mar;178(3):1406. Cowerd, Rachael B
            [corrected to Cowherd, Rachael B]]
REFERENCE   8  (bases 1 to 14218)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (bases 1 to 14218)
  AUTHORS   Brajenovic,M., Joberty,G., Kuster,B., Bouwmeester,T. and Drewes,G.
  TITLE     Comprehensive proteomic analysis of human Par protein complexes
            reveals an interconnected protein network
  JOURNAL   J Biol Chem 279 (13), 12804-12811 (2004)
   PUBMED   14676191
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL079335.29, AL391602.6, AL132768.15 and BQ027014.1.
            
            On Nov 23, 2018 this sequence version replaced NM_080627.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000237536.9/ ENSP00000237536.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-881               AL079335.29        23771-24651         c
            882-1106            AL079335.29        330-554             c
            1107-1265           AL391602.6         4435-4593           c
            1266-1328           AL132768.15        116517-116579       c
            1329-2510           AL132768.15        114236-115417       c
            2511-2651           AL132768.15        111814-111954       c
            2652-2777           AL132768.15        109092-109217       c
            2778-3008           AL132768.15        107623-107853       c
            3009-3113           AL132768.15        104963-105067       c
            3114-3281           AL132768.15        103845-104012       c
            3282-3533           AL132768.15        101966-102217       c
            3534-3605           AL132768.15        96612-96683         c
            3606-3676           AL132768.15        95992-96062         c
            3677-5027           AL132768.15        92359-93709         c
            5028-13771          AL132768.15        77004-85747         c
            13772-14218         BQ027014.1         24-470              c
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.23"
     gene            1..14218
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /note="microtubule crosslinking factor 2"
                     /db_xref="GeneID:140710"
                     /db_xref="HGNC:HGNC:16111"
                     /db_xref="MIM:620225"
     exon            1..881
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..5180
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     suppressor of glucose by autophagy; microtubule
                     cross-linking factor 2; suppressor of glucose, autophagy
                     associated 1; suppressor of glucose from autophagy; SOGA
                     family member 1; suppressor of glucose,
                     autophagy-associated protein 1; protein SOGA1"
                     /codon_start=1
                     /product="microtubule cross-linking factor 2 isoform 1"
                     /protein_id="NP_542194.2"
                     /db_xref="CCDS:CCDS54459.1"
                     /db_xref="GeneID:140710"
                     /db_xref="HGNC:HGNC:16111"
                     /db_xref="MIM:620225"
                     /translation="MEAPAAEPPVRGCGPQPAPAPAPAPERKKSHRAPSPARPKDVAG
                     WSLAKGRRGPGPGSAVACSAAFSSRPDKKGRAVAPGARGAGVRVAGVRTGVRAKGRPR
                     SGAGPRPPPPPPSLTDSSSEVSDCASEEARLLGLELALSSDAESAAGGPAGVRTGQPA
                     QPAPSAQQPPRPPASPDEPSVAASSVGSSRLPLSASLAFSDLTEEMLDCGPSGLVREL
                     EELRSENDYLKDEIEELRAEMLEMRDVYMEEDVYQLQELRQQLDQASKTCRILQYRLR
                     KAERRSLRAAQTGQVDGELIRGLEQDVKVSKDISMRLHKELEVVEKKRARLEEENEEL
                     RQRLIETELAKQVLQTELERPREHSLKKRGTRSLGKADKKTLVQEDSADLKCQLHFAK
                     EESALMCKKLTKLAKENDSMKEELLKYRSLYGDLDSALSAEELADAPHSRETELKVHL
                     KLVEEEANLLSRRIVELEVENRGLRAEMDDMKDHGGGCGGPEARLAFSALGGGECGES
                     LAELRRHLQFVEEEAELLRRSSAELEDQNKLLLNELAKFRSEHELDVALSEDSCSVLS
                     EPSQEELAAAKLQIGELSGKVKKLQYENRVLLSNLQRCDLASCQSTRPMLETDAEAGD
                     SAQCVPAPLGETHESHAVRLCRAREAEVLPGLREQAALVSKAIDVLVADANGFTAGLR
                     LCLDNECADFRLHEAPDNSEGPRDTKLIHAILVRLSVLQQELNAFTRKADAVLGCSVK
                     EQQESFSSLPPLGSQGLSKEILLAKDLGSDFQPPDFRDLPEWEPRIREAFRTGDLDSK
                     PDPSRSFRPYRAEDNDSYASEIKELQLVLAEAHDSLRGLQEQLSQERQLRKEEADNFN
                     QKMVQLKEDQQRALLRREFELQSLSLQRRLEQKFWSQEKNMLVQESQQFKHNFLLLFM
                     KLRWFLKRWRQGKVLPSEGDDFLEVNSMKELYLLMEEEEINAQHSDNKACTGDSWTQN
                     TPNEYIKTLADMKVTLKELCWLLRDERRGLTELQQQFAKAKATWETERAELKGHTSQM
                     ELKTGKGAGERAGPDWKAALQREREEQQHLLAESYSAVMELTRQLQISERNWSQEKLQ
                     LVERLQGEKQQVEQQVKELQNRLSQLQKAADPWVLKHSELEKQDNSWKETRSEKIHDK
                     EAVSEVELGGNGLKRTKSVSSMSEFESLLDCSPYLAGGDARGKKLPNNPAFGFVSSEP
                     GDPEKDTKEKPGLSSRDCNHLGALACQDPPGRQMQRSYTAPDKTGIRVYYSPPVARRL
                     GVPVVHDKEGKIIIEPGFLFTTAKPKESAEADGLAESSYGRWLCNFSRQRLDGGSAGS
                     PSAAGPGFPAALHDFEMSGNMSDDMKEITNCVRQAMRSGSLERKVKSTSSQTVGLASV
                     GTQTIRTVSVGLQTDPPRSSLHGKAWSPRSSSLVSVRSKQISSSLDKVHSRIERPCCS
                     PKYGSPKLQRRSVSKLDSSKDRSLWNLHQGKQNGSAWARSTTTRDSPVLRNINDGLSS
                     LFSVVEHSGSTESVWKLGMSETRAKPEPPKYGIVQEFFRNVCGRAPSPTSSAGEEGTK
                     KPEPLSPASYHQPEGVARILNKKAAKLGSSEEVRLTMLPQVGKDGVLRDGDGAVVLPN
                     EDAVCDCSTQSLTSCFARSSRSAIRHSPSKCRLHPSESSWGGEERALPPSE"
     exon            882..1106
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1265
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1266..1328
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..2510
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2651
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2777
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            2778..3008
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3113
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3114..3281
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3282..3533
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3534..3605
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3606..3676
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            3677..5027
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     exon            5028..14218
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      14199..14204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /note="hexamer: AATAAA"
     polyA_site      14218
                     /gene="MTCL2"
                     /gene_synonym="C20orf117; KIAA0889; SOGA; SOGA1"
                     /note="major polyA site"
ORIGIN      
        1 gcactctccg agcccagagc agcggagaga ccgcgcggcg gggcttggga cggggacgcg
       61 gtgtcggggc cgaggtgggc cgagagcagc cgcagcccca gcggccggag ccgcgccggg
      121 tccctcccca gggacgtcga ggccgggcgg gcctgggcca tgcgcgcagc tcgctgaggc
      181 cgagggaggc cgcgatggag gcgccggcgg ccgagccccc ggtccggggc tgcggtcccc
      241 agcccgcgcc tgcacccgcg cctgccccgg agaggaaaaa gagccaccgc gcgccgtcgc
      301 cggcccggcc caaagacgtg gccggctggt cgctggctaa gggccgccgc ggcccaggcc
      361 cgggctcagc agtcgcctgc agcgccgcgt tctcctcacg gccggacaag aaggggcgcg
      421 cagtggctcc cggggcgcgc ggtgcgggtg tgcgggtggc cggggtccgc accggggtcc
      481 gtgccaaggg ccgtccgcgc tcgggtgcgg ggccgcgacc gccaccgccg ccccccagcc
      541 tcacggatag cagctcggag gtgtcggact gcgcgtcgga ggaggcgcgc ctgctgggcc
      601 tggagctggc gctgagcagc gacgccgagt ccgcggccgg gggcccggcg ggggtccgta
      661 cggggcagcc ggcccagccc gcgccctccg cgcagcagcc cccgcggccg cccgcctccc
      721 cggacgagcc gtcggtggcc gcgtcgtcgg tgggcagcag ccgcttgccg ctcagcgcct
      781 cgcttgcctt ctccgacctc accgaggaga tgctggactg cgggcccagc ggcttggtgc
      841 gggagctgga ggagctgcgc tcggagaacg actatctcaa ggacgagatt gaggagctgc
      901 gggccgagat gctggagatg cgggacgtct atatggagga ggacgtgtat cagctgcagg
      961 agctgcgaca gcagctggac caggccagca agacctgccg catcctgcag taccggctgc
     1021 gcaaagccga gcgccgcagt ctccgtgccg cccagaccgg ccaggtggac ggcgagctta
     1081 tccgtggtct ggagcaggat gtcaaggtct ctaaggacat ctccatgcgg ctgcataagg
     1141 agctcgaggt ggtggagaag aaacgggcgc ggctggagga ggagaacgaa gagcttcgtc
     1201 agcggctcat cgagactgag ctggctaagc aggtgctgca gacggagctg gagcgaccga
     1261 gagagcattc cttgaagaaa agaggaaccc gctccctggg gaaggccgat aagaagactt
     1321 tggtgcagga ggacagtgca gacctgaagt gccagttgca ctttgcaaag gaggagtcag
     1381 ccctcatgtg caagaagctc actaagcttg ccaaggagaa tgacagcatg aaggaggagc
     1441 tgctgaagta ccgctcgctc tatggggacc tggacagcgc gctgtcagcc gaggagctgg
     1501 ccgatgcccc ccactcgcgg gagaccgagc tgaaggtgca cctgaagctg gtggaggagg
     1561 aagccaacct gctgagccgc cgcatcgtgg agctggaggt ggagaaccga ggcctgcggg
     1621 ctgagatgga cgacatgaag gatcatggag gtggctgtgg gggtcctgag gcacgcctgg
     1681 ccttctccgc gctgggtggc ggagagtgcg gggagagctt ggcagagctg cggcgacacc
     1741 tgcagtttgt cgaagaggag gccgagctgc tgcggcgctc ctctgccgag ctcgaggacc
     1801 agaacaagct gctgctgaac gagctggcca agttccgctc ggagcacgag ctggacgtgg
     1861 cgctgtcgga ggacagttgt tctgtgctca gcgaaccttc acaggaggag ctggcggccg
     1921 ccaagctgca gatcggcgag ctcagcggca aggtcaagaa gctgcagtac gagaaccgcg
     1981 tgctcctctc caacctccag cgctgtgacc tcgcctcctg ccagagtacg cggcccatgc
     2041 tggagacgga cgccgaggcc ggggactctg cccagtgtgt gcctgctccc ctgggcgaga
     2101 cacacgagtc ccatgcggtc cgactctgca gagccaggga ggccgaggtg ctgcctgggc
     2161 tgagagagca ggccgccctg gtcagtaagg ccatcgatgt cctggtggct gatgccaatg
     2221 gcttcacggc tggcctccgg ctgtgtctgg acaacgagtg tgctgacttc cggctgcatg
     2281 aggcccccga caacagcgag ggccccaggg acaccaagct catccatgcc atcctggtgc
     2341 gcctgagcgt gctgcagcag gagctgaatg ccttcacgcg gaaggcagat gcagtcctcg
     2401 ggtgctctgt caaggaacag caggagtcct tctcatcact gccccccttg ggctcccagg
     2461 ggctctctaa ggagattctt ctggcaaaag accttggctc agactttcag ccacctgact
     2521 tcagggacct gccggaatgg gagcccagga tccgagaggc tttccgcact ggtgacttgg
     2581 actctaagcc cgaccccagc cggagcttca ggccttaccg agctgaagac aatgattcct
     2641 atgcctctga gatcaaggag ctgcagctgg tgctggctga ggcccacgac agcctccggg
     2701 gcttgcaaga gcagctctcc caggagcggc agctacgaaa ggaggaggcc gacaatttca
     2761 accagaaaat ggtccagctg aaggaggacc agcagagggc gctcctgagg cgggagtttg
     2821 agctgcagag tctgagcctc cagcggaggc tggagcagaa attctggagc caggagaaga
     2881 acatgctggt gcaggagtcc cagcaattca agcacaactt cctgctgctc ttcatgaagc
     2941 tcaggtggtt cctcaagcgc tggcggcagg gcaaggtttt gcccagcgaa ggggatgact
     3001 tcctcgaggt gaacagcatg aaggagctgt acttgctgat ggaggaagag gagataaacg
     3061 ctcagcattc tgataacaag gcctgcacgg gggacagctg gacccagaac acgcccaatg
     3121 agtacatcaa gacactggcc gacatgaagg tgacgctgaa ggagctgtgc tggctgctcc
     3181 gggatgaacg ccgtggtctg acggagcttc agcaacagtt tgccaaggcc aaggctacct
     3241 gggagacaga gcgggcagag ctcaagggcc atacctccca gatggagctg aagacaggga
     3301 agggggccgg ggagcgggca gggcccgact ggaaggcagc cctacagcgg gagcgtgagg
     3361 agcagcagca cctcctagct gagtcctaca gcgctgtcat ggagctgact cggcagctgc
     3421 agatcagtga gcgcaactgg agccaggaaa agctgcagct ggtggagcgg ctgcagggtg
     3481 agaagcagca ggtggagcag caggtgaagg agctgcagaa ccgcctaagc cagctgcaga
     3541 aggctgccga cccctgggtc ctgaagcact cggagctgga gaagcaggac aacagctgga
     3601 aggagacacg cagtgagaag atccacgaca aggaggctgt ttccgaagtt gagcttggag
     3661 gaaatggttt aaagagaacc aaatctgttt cttccatgtc tgagtttgaa agtttgctcg
     3721 actgttcccc ttaccttgct ggcggagatg cccggggcaa gaagctgcct aacaaccctg
     3781 cctttggctt tgtgagctcc gagccagggg atccagagaa agacaccaag gagaagcctg
     3841 ggctctcgtc gagggactgc aaccacctgg gtgccctggc ctgccaggac cccccaggga
     3901 ggcagatgca gcgcagctac acggctcctg acaagacggg catccgagtc tactatagtc
     3961 ccccggtggc ccggcgcctc ggagtccctg tggttcatga caaagagggc aagatcatta
     4021 tcgagcccgg cttcctcttc accacagcca agcccaaaga gtcggccgag gctgatgggc
     4081 tggctgagag ctcctatggt cggtggctct gcaacttctc acggcagcgc ctggacggag
     4141 gctcagcggg cagcccctcg gcggccgggc ctggcttccc agcggccctg catgactttg
     4201 agatgtcagg caacatgagt gatgacatga aggagatcac caactgtgtg cgccaggcca
     4261 tgcgctccgg ctcactggag aggaaagtga agagcacatc cagccagacg gtgggcctgg
     4321 ccagtgtggg cacacagacc atccgcacgg tcagcgtggg cctgcagacc gacccacccc
     4381 gcagcagcct ccatggcaag gcctggtcac cccgcagctc ttcgctcgtg tctgtgcgca
     4441 gcaagcagat ctcctcctcc ctggacaagg tccattcgcg catcgagcgg ccctgctgct
     4501 cccccaagta tggctcacca aagctccaga ggcggtctgt gtccaagctg gacagcagca
     4561 aggaccgcag cctgtggaac ctgcaccagg gcaagcagaa cggctcggcc tgggcccgct
     4621 ccaccaccac gcgggacagc cctgtattga gaaacatcaa cgatggactc tccagcctct
     4681 tcagtgtggt ggagcactca gggagcacgg agtctgtctg gaaactaggc atgtctgaga
     4741 cgcgggccaa gcccgagcct cccaagtacg gcattgtgca ggaattcttc cgtaatgtgt
     4801 gtggccgggc accgagcccc acctcatcag caggagagga gggcaccaag aagccagagc
     4861 ccctctcccc agccagctac catcagccag agggtgtggc caggatcctg aacaagaagg
     4921 cagccaagtt gggcagcagt gaggaggtca gactcaccat gctcccccag gtggggaagg
     4981 atggtgtcct ccgggacgga gatggagccg tggtccttcc caatgaggac gctgtttgtg
     5041 actgtagtac ccagtctctc acctcctgct tcgcccgatc gtcccgctct gccatccgcc
     5101 actctccttc caagtgcagg ctgcaccctt cagagtccag ctggggtggg gaggagaggg
     5161 cactcccccc cagcgagtga cagagcagcc aagctccccg cctcaaccag cccagcccct
     5221 ggatagcaga agggaaccag cagagacgag acgaggtgag gcgaggggct gtgtcctcag
     5281 cattgcctgg ccctggaggg acagcagtga tgccactgcc agaatgcagc tttcacatca
     5341 aggtaaagcc gggtctcctg ctggcccctg ggtggtgagc ttcgacttcc caggggaagg
     5401 cagtgagtgg gagagagacc aaacctgggc ttcccaagca tccactgaga gatctgtcaa
     5461 gagccgatcc ctgggtccta agagagagcc ttgcctggtt ctgcccatgc caccctcttg
     5521 gaagagccca agaaggatac atgtctggcc atgcctttgg ggaaaaggag tcggagagat
     5581 gtttcctgct gaccatccac cccttcattt gggaggagac actgctgaga agaacaggct
     5641 ttgctctagg gctccatgtt tggttcctgg tggagccctg ttgggcatca tcaccatcac
     5701 ctccttctct ccaccacctc ctcctcccag ccccactgct ctagattgct gggacactag
     5761 ggagtatgat agggcagtag ccagggccat tgcttagtgt cctggagccc tggatctccc
     5821 tgcccatagc ctggatgcag caagagctgg gaggcgaagt ggaaacatgc agggctcagg
     5881 gttggggagt gattgcaatt gccttccttg ccaaagtgac ttggggcccc aacgttccca
     5941 gcagacccct tgaggacaga aataggtaga gtcagtctca agacctggtg catagataaa
     6001 tgcctaaata cactgccttg atctcaagtg atctcagagg cctctttccc tggcaccctg
     6061 agaggcagca ggcactacat ctccactgtg tttacatcct gcagctggtc ggggggcaaa
     6121 gatattccca gtaagagatt cttggttggc caggtcaggc ccaggagaac accaagaggc
     6181 cagagcccag gacacagcag tagactgggg cctggaaaca cgtatcttgc ctagattgtt
     6241 tatttgaatt tttcctacta taaatattta aggtggttta ctttatttta ataatttaat
     6301 ttaccccaaa gtccctaagg taatttattg gaggttgaaa catgcattct tgccactggg
     6361 acaacatgag gcctctaaca gcacgggcag gcatggggtc ccctgggtgg acgaggccgc
     6421 ttggcagcca ggtttggaga cctggcctcc tggtcagctt tggagggccc ctcaacagag
     6481 ctggagccct gcaccccaac acggctggcc atgtggcctc agaacactac ttattactca
     6541 atgcctggta cttggccagc cccagcggtc agtctataaa tactcactga caaggtggag
     6601 ggctggacgg ccatcaccac tccccagacg ttctccattg cctgtctcat ttccctccct
     6661 ccctctgaca cctttcttca tgagtcgaac gtggattact aaagctctat taagagtgtg
     6721 gagatccctc caagtttccc aaatgagaac tcacaggaaa acaggactga actttgagaa
     6781 tgttgtttat cgcagctttg cacataaacc tgagtgtctc ccagcctgcc tcggttctca
     6841 ccagcctgcc agccttttca ccagcctctc tccttagcct tatggccttt cacggctctt
     6901 ctccctgccc cagctctgct gcccgccctt cctcacgtcc cctgtgagct gcctgagcca
     6961 ttggttggat ttcgatgtgg ctcattgcag catgtggggc agcgcctccc atggcctcgc
     7021 cttggtgccg gtgaacccct tttggttgca cacatgctcc ccacacacac atagacatca
     7081 gccttcctgt aactgactgg ggacccagag tggaaacacc aggatggatc agcttgtctg
     7141 cagaattgcc catcaggaag accaaaagcc agtagctttg ctgatctgcc cccaggactc
     7201 tggaggcgcc cctgcactcc cacctcccac ctgccagttc ccagacccac ccattcggga
     7261 tcacctggac cagttacccc aagtcctgca tctcccttcc ctgcaggctg aacaccaggg
     7321 tcatgccagt cccgccagcc gcctcctcca tgccccagtg actggtgtgg gcagagcagg
     7381 cagccagtgg agctgtgggc cagttccgct cttggatgct gctgctctca cccatgaggt
     7441 caggggggcc ctccaaggtt atctccaggt gaggggattc acatcaggcc acaagccacc
     7501 agaggccttc tgccacctcc cagagcgaca gccagggagg ctgcgtactc agcctcgggg
     7561 agaaatcccc gtgggacctg agccccaaga cctacggacc actcagcctt accatcgtac
     7621 cgtccaggat tgtccttgcc atctttgttg tctcagccag accttggttt tcagtaaagc
     7681 cccagtttct acttcctgca tgccactgtg caaggccact catcactgtt cctgcagaag
     7741 cctctggacg tggggctgga tggggttgaa aatgttacat gtaaatattg gtttggttcg
     7801 gtttttagca ttttacttgg taactggttg ttttcttttt tggggtgggg ggattggttt
     7861 gtaaaaattc tctactcttt tggaatgtga tttctaagtt tgttggtttc ttcaaatgcc
     7921 ttttaagtct tggtaacatt cccaaagcag aaaactgcct gacccacagt ggggattccc
     7981 tggagaattg gggtcccaag aaggaatgct gcccttctcg aacccgttct cccccttcct
     8041 cctgcctctc tgccttttac tgctattccc ttcttctcct cctttatcct tctttctgtt
     8101 ttccccatct ccactctctc ttcaaccaaa gtcccaagga accctcgggg ctcaatcccc
     8161 catagaccac ttggcttggg tccatggggt tggcatcagt tggttggcgg aaatggggga
     8221 ccagttggca tgatggccct aaactgggaa acctcatgtt tcttatgtct cacctctttc
     8281 cagagccaaa tcagcccctt ttggaatgat gacttcattg gaatgcaaat caagtcattt
     8341 tggtgcatca gtggctctta ggcctgcaca cacgagacat cagaatccaa tcctctgacc
     8401 ctgtgccagc cctttccccc agtttatttc ccaccaaagg ctgacctcta agaggtcttg
     8461 ctttctatga actcaagatg ggtcccacct ctaggtgtcc ccaggtgcac tcttctaccg
     8521 gttggcttcc gatgtgacaa ggccaagggc ccaaagactt gaccctctta cacccttgct
     8581 gacatggttc catcatgtcc acccgcatgc acttttatgg tttcatcacc cagcctcttc
     8641 tcctctggcc cacccagcgt ccaggctctt tctccctctc ccctcctatc tagaatgtcc
     8701 cctgcttcta gcctcaccag accccccaag ctcccactac ttcttccata ataatagtaa
     8761 taacaatggt tatcatcatc ccctgcacat cccgcctaaa gcactttact atatagaaaa
     8821 cgtttcccct ggccgggcat ggtggctcac gcctgaaatc ccagctcttt gggaggctga
     8881 ggcgagcgga tcagttgagg tcaggagttc aacgccagcc tggccaacgt ggtgaatccc
     8941 tgtctgtact aaaagtacaa aaaattagct gagcatggtg gtgcgtgcct gtaatcccag
     9001 ctactcggga ggctgaggtg ggagaatcgc ttgagcccag gaggcggagg ttgcaggagc
     9061 agagattgcg ccactgcact ccagcctgga tgacagagtg agacccaatc tcaaaaaaga
     9121 aatcgtttcc caccccacat ctccttcaga cctctcaggg acaactctgg gaggcagcct
     9181 tggcaggaca tgggttagtg cgcccatttt gctgtgagga aactgaggta caggtctcat
     9241 cccagagcat gagaagtcac tgagtttaga tgagaacttg ggtccaactc tgtcctgttt
     9301 gctgtgcaaa tccgctgccc tgctgggggc ttttggtggg tccagaatac ccagaatatg
     9361 ctgctggcca acccaggcat aaaacaagtc cattctagat cactgagcct tgtgtattcc
     9421 agagggtgat ctgaggtccc cattcagcag aattctctga gggcatgttc agaatgtaga
     9481 ttcctgggcc ccaccttgaa tttgcatgtt taacaaactc tcctggggtt gaggggtggg
     9541 tgcagtggtc acacctgtaa tcccagcact ttgggaggct gaggcgggtg gatcacttga
     9601 gctcaggagt tcgagcctgg ccaatatggt gaaaccctgt ctctactaaa aatgcaaaaa
     9661 ttagccaggt gtggtggcac atgcctgtaa tcccagctac ttgggaggct gaggcaggag
     9721 aatcacttga acctggtggg gagcagcggt tgcagtgagc cgagattgtg ccattgcgct
     9781 ctagcctggg caacggaatg agacttgtct caaaaaaaat aaaaaataaa accagccctc
     9841 cccgggggat cttaggcact attggccaca ccattggtgt tcgtggccct gattattagg
     9901 ctatctttct ttttttaagt ttttttagat ttatttttta ttttatttat ttatttattt
     9961 attatttatt attattattt ttagagacag ggggtctccc tatgttgccc aggctggttt
    10021 caaactcctg ggctcaagtg atctgccctc ctcagcctcc caaagtgctg gggttacagg
    10081 caggcatcag ccaccgtgcc aggtcatctt cctttttctt ttttttttgg agacagagtc
    10141 ttgctctgtc attcaggctg gagtacagtg gcgccatttc agctcaccgc agcctccacc
    10201 tcccaggttc aagcagttct cctgcctcaa cctcccgagt aactgggatt acaggtgtgt
    10261 gccaccacac ccgactaatt tttttatttt tagtagagac ggggtttcac catgttggcc
    10321 aggctggtct tgaactcctg acctcaggtg atccacccac ctcggcctcc caaagtactg
    10381 ggattatagg cgtgagccac ggcatccagc ctcatctttc ttttaaccaa taaacatgat
    10441 gctgtatctt aaaaagagca ctgagcaggg acttaaggga tcgagtcctc aaccaaactg
    10501 atttaattac tcaggatttt caaaaagcat cagaggctat ttacaatctt aatcataggg
    10561 gttcagtaaa ataaaaataa gaagtaaaaa agcaagagaa attattctgt aaatctaact
    10621 ggtgtaattc ccataatcat gcaattaagt tttactcttg agtttcctga cagccattgg
    10681 taaaaagaga aacacatcag gatttataat ttttatcatc caattatggg aagcaagcat
    10741 gttggcccca ggagacgaac tcttctacta atttatagca tgtatccttt catcaagggc
    10801 cactgagcca gttggtgagt caacgggtga acctaagatg caaggatgtt ttccaggtga
    10861 ctatttaaaa aaataaataa tccaccaacg tgattgacct tggcgagatc atgtttctag
    10921 tctatacctc agtttcccca tctgtaaagt gaggataatg tcccacccca tgtaactgtg
    10981 gtgaggacca actgcaacac tgtgcctgcg agtctccttg gaaaagtgta aggttctaca
    11041 caaatggaaa gtgatctgat cacactcagt gtccccagcc cagcctttca gtgccctggc
    11101 cctggggtgg gggacaatac tctcctcacc cccttcacta gtcttcatga atagcaagga
    11161 ggccataaca taatttggtc taaacccctt cctttttaaa agaatgatgg caaaatgtgc
    11221 ataacattaa atttaccatt ttaaagattt ctaagtgtct cgaagtacat ttgcaatgtg
    11281 taactgccac ctccagaact ttttcatcat cctaaactga aactctgtac ccactaaaca
    11341 gtaactcccc actccccctg tccccagtcc ctggtaacct ctattctact tttttttttt
    11401 ctctgtggat tggcctattc taggtatctc ttttaagtag aatcatatag tatttgtcct
    11461 tttgtgtcca gccccctcat tttttgagat gaggaatcag gcccagagaa ggcagtggct
    11521 cacccaaggt cacatcgcaa accagaggca gagccaagac cagaacccag gtttcctgac
    11581 tcctaacagg tcttgcatcc ccagtagtga atgagacact gctttgactt tctgtaatct
    11641 tggtttagcc ccttcctttc tctgggctca gtctgcttcc caccactcac ccattctttc
    11701 tttatcatct ccctccaaag cctcttgtcc tcctgcctcc tcttctcctt ggctggttcc
    11761 tgccagcaaa tggagtggcc ctggtccatg ccaggttttc ctcttctggg tccggagctc
    11821 actatagtat tcagccctca gtcctcccag gatgttcttc ccagcactgc tgcctcgtgc
    11881 ggattttccc gtaacctcag taactggctt cttgtccccc tgcttcctac cagggaagcc
    11941 ttcctgtccg cgtctgtggt ttcccaactc tgatatttgc tctcaaatgt ggtggtgtcc
    12001 tggttctgtg tttatttatt ttgtgttttc tcacacacac aaaaaaaagc ctcttcctcc
    12061 aatgcatcag gaggcaccag ccctgccagc ccttctcact gggctcaccc tgccccagca
    12121 accccccggt gccaccctta agccacactg tcttctctgt aagcagcctg ccagcagcag
    12181 ccccagcact ttgcaatggg cgtgtgtgtg gtggtgggtg gggggggctt ggatccctcc
    12241 tttttcctcc cctgccctgc ccaggcccag atggccttga ctgtaaagca ggtgctgcct
    12301 gacaggttct tctctccctg tctctggtca ttgatccatc tctttgtcca ttcagtatcc
    12361 aaccatcctc tccattctcc tctggacctc accactctca gagctgcttg tcctggcaga
    12421 atctacagtt caccccaact ctatgcctta cccctcccaa cccaacagca tttgcagttt
    12481 gcaaaatata cagacccaag tcctgagggg actgaggaca tgatgctggg cccaagtctc
    12541 ctgctcaggg cttctctcca atgccagccc tgccactcct tcctcaccct ccttggagcc
    12601 tcctctgctg cttgtctatc ccaacggccc tgctcccctc ccttcctgcc cttcaccagc
    12661 tttctggaca ccatgccctg aggaagggac ctttggtttt ctctaaacat ctttgaaggg
    12721 ctgaggcagt cagggctggc tgccttgtca ctctttattt ggaagccact caaaccattc
    12781 ccaagaagag ggacctcagc tggcaatctg gaaacctggc ccaggtctgg gcagatgtct
    12841 tcacttctcc taccttccca gtcttgtgat cctgtgatga gcaccaggat ggccctgtgg
    12901 tccctagagc acccctcatg ctgtagggtc ctgcagcccc atcctttctc tactgggccc
    12961 tggtatcctg gctcctctct cagctctgcc actgatctct gtgccttagt ttacttctct
    13021 gcacggggga ctcaccccaa gaccatttcc agcagcttcc caggtgatgt ggtgccccaa
    13081 ggctgggctt tgccagctgt ggcccagctc cttagtgctg cccaggagac accaggctgc
    13141 tcagaatgag gtgactgcgg gcaccattct cagccagtgg ttcttgtatt gcattccagc
    13201 agcaggaata tcacctggga acttgataga agtgcagatt agcagcccca cccaagaccc
    13261 actgaattag agcttgtgga gtggggccct acaagctggg gttttaagga gccctccaaa
    13321 tgattctgac gcataagaat atgccaactg ctgatctggg ctagccatta gtagagcctg
    13381 gggagggact gggactggct aggccaagaa caggtggaaa acaccagcct tatctggact
    13441 cctgagattg ggaaccacca ccaacaaaaa ccaaccctat agtcgctcct cttggaagag
    13501 gaagagaagt tgaagggcct ggagaaagca cacattgttt gtttccctgc tcctgctcac
    13561 ctctctcact tgtcttggtt ttacaaaagg ctgtgtggat ggtgccagcc agggaggggg
    13621 tgggagtcct ggggaggcag gaggcagaag accctgactg tttctccctt gggaacctca
    13681 ccataggcca gatagcgcct cttcaaactg aaagaaatct taactccaca aagaaagcat
    13741 cctaaatccc cagttcctcc tcctcccaac cccagggata ccttgtagac agtgccaaaa
    13801 aacagctcca acccccagca gctgggaaga gagccagaag ctgcccttcc tcctcatcct
    13861 ggcccctccc cagcccccgc caatactgtg aacccccttc ccactcagcc tggtttcctg
    13921 gtgagggtcc tgcagtcatg ggccctgggg gacccccagg gcaaggccca tgggagggaa
    13981 gggaccaagg gcatccttgg gccaactgtc cacctctctt gtccactatt ctctcctttc
    14041 cacttctgtc ttcaaaaggc tccttcctag gatggatcgg gtgctaggac aactgcagtc
    14101 caatccacca gctctccctg cccctgtgtc ttatttcaga catgagaata actgtacagt
    14161 gtaaacttat aaagcgtttt taatggttgt agattggaaa taaagtatgt catatgaa
//
