LOCUS       NM_016332               1277 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens methionine sulfoxide reductase B1 (MSRB1), transcript
            variant 1, mRNA.
ACCESSION   NM_016332
VERSION     NM_016332.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Tarrago,L., Kaya,A., Kim,H.Y., Manta,B., Lee,B.C. and
            Gladyshev,V.N.
  TITLE     The selenoprotein methionine sulfoxide reductase B1 (MSRB1)
  JOURNAL   Free Radic Biol Med 191, 228-240 (2022)
   PUBMED   36084791
  REMARK    GeneRIF: The selenoprotein methionine sulfoxide reductase B1
            (MSRB1).
            Review article
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1277)
  AUTHORS   Li,H., He,Q., Meng,F., Feng,X., Chen,J., Li,L. and Liu,J.
  TITLE     Methionine sulfoxide reductase B1 regulates proliferation and
            invasion by affecting mitogen-activated protein kinase pathway and
            epithelial-mesenchymal transition in u2os cells
  JOURNAL   Biochem Biophys Res Commun 496 (3), 806-813 (2018)
   PUBMED   29395081
  REMARK    GeneRIF: In vivo, MsrB1 shRNAi can inhibit lung metastasis in
            metastasis model. In conclusion, MsrB1 regulates proliferation and
            invasion of u2os cells by affecting MAPK pathway and EMT, and MsrB1
            gene may be a novel therapeutic target against tumors.
REFERENCE   4  (bases 1 to 1277)
  AUTHORS   Wang,C., Chen,P., He,X., Peng,Z., Chen,S., Zhang,R., Cheng,J. and
            Liu,Q.
  TITLE     Direct interaction between selenoprotein R and Abeta42
  JOURNAL   Biochem Biophys Res Commun 489 (4), 509-514 (2017)
   PUBMED   28579431
REFERENCE   5  (bases 1 to 1277)
  AUTHORS   Achilli,C., Ciana,A., Rossi,A., Balduini,C. and Minetti,G.
  TITLE     Neutrophil granulocytes uniquely express, among human blood cells,
            high levels of Methionine-sulfoxide-reductase enzymes
  JOURNAL   J Leukoc Biol 83 (1), 181-189 (2008)
   PUBMED   17938273
  REMARK    GeneRIF: The neutrophils expressed the highest activity Msrs. Msrs
            are important as antioxidant/repair systems for neutrophils, cells
            with enormous capacity for the generation of reactive oxidants and
            hence, susceptible to oxidative damage.
REFERENCE   6  (bases 1 to 1277)
  AUTHORS   Kim,H.Y. and Gladyshev,V.N.
  TITLE     Methionine sulfoxide reduction in mammals: characterization of
            methionine-R-sulfoxide reductases
  JOURNAL   Mol Biol Cell 15 (3), 1055-1064 (2004)
   PUBMED   14699060
REFERENCE   7  (bases 1 to 1277)
  AUTHORS   Moskovitz,J., Singh,V.K., Requena,J., Wilkinson,B.J., Jayaswal,R.K.
            and Stadtman,E.R.
  TITLE     Purification and characterization of methionine sulfoxide
            reductases from mouse and Staphylococcus aureus and their substrate
            stereospecificity
  JOURNAL   Biochem Biophys Res Commun 290 (1), 62-65 (2002)
   PUBMED   11779133
  REMARK    GeneRIF: The studies in mouse show that MsrB is a selenoprotein
            that exhibits high specificity for reduction of the R forms of free
            and protein-bound methionine sulfoxide.
REFERENCE   8  (bases 1 to 1277)
  AUTHORS   Daniels,R.J., Peden,J.F., Lloyd,C., Horsley,S.W., Clark,K.,
            Tufarelli,C., Kearney,L., Buckle,V.J., Doggett,N.A., Flint,J. and
            Higgs,D.R.
  TITLE     Sequence, structure and pathology of the fully annotated terminal 2
            Mb of the short arm of human chromosome 16
  JOURNAL   Hum Mol Genet 10 (4), 339-352 (2001)
   PUBMED   11157797
REFERENCE   9  (bases 1 to 1277)
  AUTHORS   Lescure,A., Gautheret,D., Carbon,P. and Krol,A.
  TITLE     Novel selenoproteins identified in silico and in vivo by using a
            conserved RNA structural motif
  JOURNAL   J Biol Chem 274 (53), 38147-38154 (1999)
   PUBMED   10608886
REFERENCE   10 (bases 1 to 1277)
  AUTHORS   Kryukov,G.V., Kryukov,V.M. and Gladyshev,V.N.
  TITLE     New mammalian selenocysteine-containing proteins identified with an
            algorithm that searches for selenocysteine insertion sequence
            elements
  JOURNAL   J Biol Chem 274 (48), 33888-33897 (1999)
   PUBMED   10567350
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC005363.1.
            
            On Apr 24, 2020 this sequence version replaced NM_016332.3.
            
            Summary: The protein encoded by this gene belongs to the
            methionine-R-sulfoxide reductase B (MsrB) family. Members of this
            family function as repair enzymes that protect proteins from
            oxidative stress by catalyzing the reduction of
            methionine-R-sulfoxides to methionines. This protein is highly
            expressed in liver and kidney, and is localized to the nucleus and
            cytosol. It is the only member of the MsrB family that is a
            selenoprotein, containing a selenocysteine (Sec) residue at its
            active site. It also has the highest methionine-R-sulfoxide
            reductase activity compared to other members containing cysteine in
            place of Sec. Sec is encoded by the UGA codon, which normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, designated the Sec
            insertion sequence (SECIS) element, that is necessary for the
            recognition of UGA as a Sec codon, rather than as a stop signal. A
            pseudogene of this locus has been identified on chromosome 19.
            [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003127.1, AK026128.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match              :: ENST00000361871.8/
                                               ENSP00000355084.3
            protein contains selenocysteine :: PMID: 10567350, 10608886
            RefSeq Select criteria          :: based on manual assertion,
                                               conservation, expression,
                                               longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-98                AC005363.1         44786-44883         c
            99-247              AC005363.1         42941-43089         c
            248-362             AC005363.1         42462-42576         c
            363-1277            AC005363.1         39913-40827         c
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..1277
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /note="methionine sulfoxide reductase B1"
                     /db_xref="GeneID:51734"
                     /db_xref="HGNC:HGNC:14133"
                     /db_xref="MIM:606216"
     exon            1..98
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..394
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /EC_number="1.8.4.12"
                     /EC_number="1.8.4.14"
                     /note="UGA stop codon recoded as selenocysteine; isoform 1
                     is encoded by transcript variant 1; selenoprotein R;
                     methionine-R-sulfoxide reductase B1; selenoprotein X, 1"
                     /codon_start=1
                     /transl_except=(pos:326..328,aa:Sec)
                     /product="methionine-R-sulfoxide reductase B1 isoform 1"
                     /protein_id="NP_057416.1"
                     /db_xref="CCDS:CCDS42100.1"
                     /db_xref="GeneID:51734"
                     /db_xref="HGNC:HGNC:14133"
                     /db_xref="MIM:606216"
                     /translation="MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWP
                     AFTETIHADSVAKRPEHNRSEALKVSCGKCGNGLGHEFLNDGPKPGQSRFUIFSSSLK
                     FVPKGKETSASQGH"
     exon            99..247
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /inference="alignment:Splign:2.1.0"
     exon            248..362
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /inference="alignment:Splign:2.1.0"
     exon            363..1277
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      871..942
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
     regulatory      1259..1264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /note="hexamer: AATGAA"
     polyA_site      1265
                     /gene="MSRB1"
                     /gene_synonym="HSPC270; SELENOR; SELENOX; SELR; SELX;
                     SepR; SEPX1"
                     /note="major polyA site"
ORIGIN      
        1 ggtcagttgg cagcggcaag cgcgctgcgg ttccggtggc gccatgtcgt tctgcagctt
       61 cttcgggggc gaggttttcc agaatcactt tgaacctggc gtttacgtgt gtgccaagtg
      121 tggctatgag ctgttctcca gccgctcgaa gtatgcacac tcgtctccat ggccggcgtt
      181 caccgagacc attcacgccg acagcgtggc caagcgtccg gagcacaata gatctgaagc
      241 cttgaaggtg tcctgtggca agtgtggcaa tgggttgggc cacgagttcc tgaacgacgg
      301 ccccaagccg gggcagtccc gattctgaat attcagcagc tcgctgaagt ttgtccctaa
      361 aggcaaagaa acttctgcct cccagggtca ctaggcgggc agcccacacc caccccagac
      421 ggccaccaca ctgaggccac acgttggcca ttccaccttg gagttggaac cctgggcgtc
      481 gagacaggaa ggcagggcgc agtggttgaa acatcaggac actcccaagg ccccggctct
      541 gaacaagacc ttttcgtttc ttggaaaaga gactcatttg ctgatggttc atgccttctg
      601 ctgggacagg cctgggctgt gcagccacac tgtcggctga cttagccccc tgctcactct
      661 aggtgcctcc aggaggtgag ccctgggtgc agctggtctc tgaatgacgt tacaccctca
      721 ccttcttttc ctggccctgt ctctggactc tcccctgtga ggcccaattc caagacagac
      781 tctcgtcctc accgaagctt aggcccacat ctcccaggct gcttaggaga cagaatggaa
      841 acggaggccg cccctgccag ccgccctggc cctggtcact gcatgatccg ctctggtcaa
      901 acccttccag gccagccaga gtggggatgg tctgtgacct gctgggaagg caggctgatg
      961 gggcacaccc ttggcctctc gtccacgagg ggagaaacct aaaccctgtt tcacaatctg
     1021 tgcggaagta gcttgcctca cttctgctta ggaaagcggc tgttgctcca taactctaac
     1081 cagcacaggg ctgaggcctg cagtgcacac ctgcagggag gcccttccca aggtgtggtg
     1141 actgtgcctt actgtacatg ctcggaggcc tggccatata ggagggtggg tgatgctgaa
     1201 atcacccccc atcttaagta attactttct ggagtaatca ggtggaaatc catagacaaa
     1261 tgaaacattc agatgta
//
