LOCUS       NM_005823               2197 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA.
ACCESSION   NM_005823
VERSION     NM_005823.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Guo,J., Zeng,X., Zhu,Y., Yang,D. and Zhao,X.
  TITLE     Mesothelin-based CAR-T cells exhibit potent antitumor activity
            against ovarian cancer
  JOURNAL   J Transl Med 22 (1), 367 (2024)
   PUBMED   38637885
  REMARK    GeneRIF: Mesothelin-based CAR-T cells exhibit potent antitumor
            activity against ovarian cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Kitadai,R., Nishikawa,T., Yoshida,H., Mizoguchi,C., Yamamoto,K.,
            Kato,T. and Yonemori,K.
  TITLE     Mesothelin expression in gynecologic carcinosarcoma:
            clinicopathological significance and correlation with HER2
            expression
  JOURNAL   J Gynecol Oncol 35 (2), e11 (2024)
   PUBMED   37914528
  REMARK    GeneRIF: Mesothelin expression in gynecologic carcinosarcoma:
            clinicopathological significance and correlation with HER2
            expression.
REFERENCE   3  (bases 1 to 2197)
  AUTHORS   Jang,Y., Koh,J.S., Park,J.H., Choi,S., Duong,P.T.T., Heo,B.Y.,
            Lee,S.W., Kim,J.Y., Lee,M.W., Kim,S.H. and Song,I.C.
  TITLE     Enhanced Expression of Glycolytic Enzymes and Succinate
            Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes
            Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute
            Myeloid Leukemia
  JOURNAL   Int J Mol Sci 25 (4), 2140 (2024)
   PUBMED   38396817
  REMARK    GeneRIF: Enhanced Expression of Glycolytic Enzymes and Succinate
            Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes
            Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute
            Myeloid Leukemia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2197)
  AUTHORS   Liu,X.F., Onda,M., Schlomer,J., Bassel,L., Kozlov,S., Tai,C.H.,
            Zhou,Q., Liu,W., Tsao,H.E., Hassan,R., Ho,M. and Pastan,I.
  TITLE     Tumor resistance to anti-mesothelin CAR-T cells caused by binding
            to shed mesothelin is overcome by targeting a juxtamembrane epitope
  JOURNAL   Proc Natl Acad Sci U S A 121 (4), e2317283121 (2024)
   PUBMED   38227666
  REMARK    GeneRIF: Tumor resistance to anti-mesothelin CAR-T cells caused by
            binding to shed mesothelin is overcome by targeting a juxtamembrane
            epitope.
REFERENCE   5  (bases 1 to 2197)
  AUTHORS   Pastan,I. and Hassan,R.
  TITLE     Discovery of mesothelin and exploiting it as a target for
            immunotherapy
  JOURNAL   Cancer Res 74 (11), 2907-2912 (2014)
   PUBMED   24824231
  REMARK    Review article
REFERENCE   6  (bases 1 to 2197)
  AUTHORS   Scholler,N., Fu,N., Yang,Y., Ye,Z., Goodman,G.E., Hellstrom,K.E.
            and Hellstrom,I.
  TITLE     Soluble member(s) of the mesothelin/megakaryocyte potentiating
            factor family are detectable in sera from patients with ovarian
            carcinoma
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11531-11536 (1999)
   PUBMED   10500211
REFERENCE   7  (bases 1 to 2197)
  AUTHORS   Brinkmann,U., Webber,K., Di Carlo,A., Beers,R., Chowdhury,P.,
            Chang,K., Chaudhary,V., Gallo,M. and Pastan,I.
  TITLE     Cloning and expression of the recombinant FAb fragment of
            monoclonal antibody K1 that reacts with mesothelin present on
            mesotheliomas and ovarian cancers
  JOURNAL   Int J Cancer 71 (4), 638-644 (1997)
   PUBMED   9178820
REFERENCE   8  (bases 1 to 2197)
  AUTHORS   Chang,K. and Pastan,I.
  TITLE     Molecular cloning of mesothelin, a differentiation antigen present
            on mesothelium, mesotheliomas, and ovarian cancers
  JOURNAL   Proc Natl Acad Sci U S A 93 (1), 136-140 (1996)
   PUBMED   8552591
REFERENCE   9  (bases 1 to 2197)
  AUTHORS   Kojima,T., Oh-eda,M., Hattori,K., Taniguchi,Y., Tamura,M., Ochi,N.
            and Yamaguchi,N.
  TITLE     Molecular cloning and expression of megakaryocyte potentiating
            factor cDNA
  JOURNAL   J Biol Chem 270 (37), 21984-21990 (1995)
   PUBMED   7665620
REFERENCE   10 (bases 1 to 2197)
  AUTHORS   Yamaguchi,N., Hattori,K., Oh-eda,M., Kojima,T., Imai,N. and Ochi,N.
  TITLE     A novel cytokine exhibiting megakaryocyte potentiating activity
            from a human pancreatic tumor cell line HPC-Y5
  JOURNAL   J Biol Chem 269 (2), 805-808 (1994)
   PUBMED   8288629
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL031258.12, AY743922.1 and
            BC003512.1.
            
            On Nov 23, 2018 this sequence version replaced NM_005823.5.
            
            Summary: This gene encodes a preproprotein that is proteolytically
            processed to generate two protein products, megakaryocyte
            potentiating factor and mesothelin. Megakaryocyte potentiating
            factor functions as a cytokine that can stimulate colony formation
            of bone marrow megakaryocytes. Mesothelin is a
            glycosylphosphatidylinositol-anchored cell-surface protein that may
            function as a cell adhesion protein. This protein is overexpressed
            in epithelial mesotheliomas, ovarian cancers and in specific
            squamous cell carcinomas. Alternative splicing results in multiple
            transcript variants, at least one of which encodes an isoform that
            is proteolytically processed. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes isoform 1. Both variants 1 and 3 encode the
            same isoform isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY743922.1, SRR14038191.4401622.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000545450.7/ ENSP00000442965.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AL031258.12        13669-13699
            32-614              AY743922.1         1-583
            615-637             AL031258.12        17877-17899
            638-1731            AY743922.1         607-1700
            1732-1732           AL031258.12        20342-20342
            1733-1975           AY743922.1         1702-1944
            1976-1976           AL031258.12        21494-21494
            1977-2141           AY743922.1         1946-2110
            2142-2142           AL031258.12        21742-21742
            2143-2193           AY743922.1         2112-2162
            2194-2197           BC003512.1         2409-2412
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2197
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="mesothelin"
                     /db_xref="GeneID:10232"
                     /db_xref="HGNC:HGNC:7371"
                     /db_xref="MIM:601051"
     exon            1..88
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            89..190
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            191..284
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..2068
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; megakaryocyte potentiating factor; soluble MPF
                     mesothelin related protein; CAK1 antigen;
                     pre-pro-megakaryocyte-potentiating factor"
                     /codon_start=1
                     /product="mesothelin isoform 1 preproprotein"
                     /protein_id="NP_005814.2"
                     /db_xref="CCDS:CCDS45370.1"
                     /db_xref="GeneID:10232"
                     /db_xref="HGNC:HGNC:7371"
                     /db_xref="MIM:601051"
                     /translation="MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQE
                     AAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLR
                     CLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQ
                     RLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQD
                     QQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSS
                     RDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLAT
                     QMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSL
                     ETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELS
                     SVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDL
                     KALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQ
                     RQDDLDTLGLGLQGGIPNGYLVLDLSMQEALSGTPCLLGPGPVLTVLALLLASTLA"
     exon            285..328
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            329..378
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            379..499
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            500..579
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            580..709
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            710..903
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            904..994
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1094
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1273
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1429
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1572
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1700
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1795
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1796..1982
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2197
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2169..2174
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="hexamer: AGTAAA"
     polyA_site      2197
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="major polyA site"
ORIGIN      
        1 gtcatttgtt ccctttgacg gcccgggagg ctgccaggct ctccaccccc acttcccaat
       61 tgaggaaacc gaggcagagg aggctcagcg ccacgcactc ctctttctgc ctggccggcc
      121 actcccgtct gctgtgacgc gcggacagag agctaccggt ggacccacgg tgcctccctc
      181 cctgggatct acacagacca tggccttgcc aacggctcga cccctgttgg ggtcctgtgg
      241 gacccccgcc ctcggcagcc tcctgttcct gctcttcagc ctcggatggg tgcagccctc
      301 gaggaccctg gctggagaga cagggcagga ggctgcgccc ctggacggag tcctggccaa
      361 cccacctaac atttccagcc tctcccctcg ccaactcctt ggcttcccgt gtgcggaggt
      421 gtccggcctg agcacggagc gtgtccggga gctggctgtg gccttggcac agaagaatgt
      481 caagctctca acagagcagc tgcgctgtct ggctcaccgg ctctctgagc cccccgagga
      541 cctggacgcc ctcccattgg acctgctgct attcctcaac ccagatgcgt tctcggggcc
      601 ccaggcctgc acccgtttct tctcccgcat cacgaaggcc aatgtggacc tgctcccgag
      661 gggggctccc gagcgacagc ggctgctgcc tgcggctctg gcctgctggg gtgtgcgggg
      721 gtctctgctg agcgaggctg atgtgcgggc tctgggaggc ctggcttgcg acctgcctgg
      781 gcgctttgtg gccgagtcgg ccgaagtgct gctaccccgg ctggtgagct gcccgggacc
      841 cctggaccag gaccagcagg aggcagccag ggcggctctg cagggcgggg gaccccccta
      901 cggccccccg tcgacatggt ctgtctccac gatggacgct ctgcggggcc tgctgcccgt
      961 gctgggccag cccatcatcc gcagcatccc gcagggcatc gtggccgcgt ggcggcaacg
     1021 ctcctctcgg gacccatcct ggcggcagcc tgaacggacc atcctccggc cgcggttccg
     1081 gcgggaagtg gagaagacag cctgtccttc aggcaagaag gcccgcgaga tagacgagag
     1141 cctcatcttc tacaagaagt gggagctgga agcctgcgtg gatgcggccc tgctggccac
     1201 ccagatggac cgcgtgaacg ccatcccctt cacctacgag cagctggacg tcctaaagca
     1261 taaactggat gagctctacc cacaaggtta ccccgagtct gtgatccagc acctgggcta
     1321 cctcttcctc aagatgagcc ctgaggacat tcgcaagtgg aatgtgacgt ccctggagac
     1381 cctgaaggct ttgcttgaag tcaacaaagg gcacgaaatg agtcctcagg tggccaccct
     1441 gatcgaccgc tttgtgaagg gaaggggcca gctagacaaa gacaccctag acaccctgac
     1501 cgccttctac cctgggtacc tgtgctccct cagccccgag gagctgagct ccgtgccccc
     1561 cagcagcatc tgggcggtca ggccccagga cctggacacg tgtgacccaa ggcagctgga
     1621 cgtcctctat cccaaggccc gccttgcttt ccagaacatg aacgggtccg aatacttcgt
     1681 gaagatccag tccttcctgg gtggggcccc cacggaggat ttgaaggcgc tcagtcagca
     1741 gaatgtgagc atggacttgg ccacgttcat gaagctgcgg acggatgcgg tgctgccgtt
     1801 gactgtggct gaggtgcaga aacttctggg accccacgtg gagggcctga aggcggagga
     1861 gcggcaccgc ccggtgcggg actggatcct acggcagcgg caggacgacc tggacacgct
     1921 ggggctgggg ctacagggcg gcatccccaa cggctacctg gtcctagacc tcagcatgca
     1981 agaggccctc tcggggacgc cctgcctcct aggacctgga cctgttctca ccgtcctggc
     2041 actgctccta gcctccaccc tggcctgagg gccccactcc cttgctggcc ccagccctgc
     2101 tggggatccc cgcctggcca ggagcaggca cgggtggtcc ccgttccacc ccaagagaac
     2161 tcgcgctcag taaacgggaa catgccccct gcagaca
//
