LOCUS       NM_001177355            2137 bp    mRNA    linear   PRI 03-AUG-2024
DEFINITION  Homo sapiens mesothelin (MSLN), transcript variant 3, mRNA.
ACCESSION   NM_001177355
VERSION     NM_001177355.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Guo,J., Zeng,X., Zhu,Y., Yang,D. and Zhao,X.
  TITLE     Mesothelin-based CAR-T cells exhibit potent antitumor activity
            against ovarian cancer
  JOURNAL   J Transl Med 22 (1), 367 (2024)
   PUBMED   38637885
  REMARK    GeneRIF: Mesothelin-based CAR-T cells exhibit potent antitumor
            activity against ovarian cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Kitadai,R., Nishikawa,T., Yoshida,H., Mizoguchi,C., Yamamoto,K.,
            Kato,T. and Yonemori,K.
  TITLE     Mesothelin expression in gynecologic carcinosarcoma:
            clinicopathological significance and correlation with HER2
            expression
  JOURNAL   J Gynecol Oncol 35 (2), e11 (2024)
   PUBMED   37914528
  REMARK    GeneRIF: Mesothelin expression in gynecologic carcinosarcoma:
            clinicopathological significance and correlation with HER2
            expression.
REFERENCE   3  (bases 1 to 2137)
  AUTHORS   Jang,Y., Koh,J.S., Park,J.H., Choi,S., Duong,P.T.T., Heo,B.Y.,
            Lee,S.W., Kim,J.Y., Lee,M.W., Kim,S.H. and Song,I.C.
  TITLE     Enhanced Expression of Glycolytic Enzymes and Succinate
            Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes
            Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute
            Myeloid Leukemia
  JOURNAL   Int J Mol Sci 25 (4), 2140 (2024)
   PUBMED   38396817
  REMARK    GeneRIF: Enhanced Expression of Glycolytic Enzymes and Succinate
            Dehydrogenase Complex Flavoprotein Subunit A by Mesothelin Promotes
            Glycolysis and Mitochondrial Respiration in Myeloblasts of Acute
            Myeloid Leukemia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2137)
  AUTHORS   Liu,X.F., Onda,M., Schlomer,J., Bassel,L., Kozlov,S., Tai,C.H.,
            Zhou,Q., Liu,W., Tsao,H.E., Hassan,R., Ho,M. and Pastan,I.
  TITLE     Tumor resistance to anti-mesothelin CAR-T cells caused by binding
            to shed mesothelin is overcome by targeting a juxtamembrane epitope
  JOURNAL   Proc Natl Acad Sci U S A 121 (4), e2317283121 (2024)
   PUBMED   38227666
  REMARK    GeneRIF: Tumor resistance to anti-mesothelin CAR-T cells caused by
            binding to shed mesothelin is overcome by targeting a juxtamembrane
            epitope.
REFERENCE   5  (bases 1 to 2137)
  AUTHORS   Pastan,I. and Hassan,R.
  TITLE     Discovery of mesothelin and exploiting it as a target for
            immunotherapy
  JOURNAL   Cancer Res 74 (11), 2907-2912 (2014)
   PUBMED   24824231
  REMARK    Review article
REFERENCE   6  (bases 1 to 2137)
  AUTHORS   Scholler,N., Fu,N., Yang,Y., Ye,Z., Goodman,G.E., Hellstrom,K.E.
            and Hellstrom,I.
  TITLE     Soluble member(s) of the mesothelin/megakaryocyte potentiating
            factor family are detectable in sera from patients with ovarian
            carcinoma
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11531-11536 (1999)
   PUBMED   10500211
REFERENCE   7  (bases 1 to 2137)
  AUTHORS   Brinkmann,U., Webber,K., Di Carlo,A., Beers,R., Chowdhury,P.,
            Chang,K., Chaudhary,V., Gallo,M. and Pastan,I.
  TITLE     Cloning and expression of the recombinant FAb fragment of
            monoclonal antibody K1 that reacts with mesothelin present on
            mesotheliomas and ovarian cancers
  JOURNAL   Int J Cancer 71 (4), 638-644 (1997)
   PUBMED   9178820
REFERENCE   8  (bases 1 to 2137)
  AUTHORS   Chang,K. and Pastan,I.
  TITLE     Molecular cloning of mesothelin, a differentiation antigen present
            on mesothelium, mesotheliomas, and ovarian cancers
  JOURNAL   Proc Natl Acad Sci U S A 93 (1), 136-140 (1996)
   PUBMED   8552591
REFERENCE   9  (bases 1 to 2137)
  AUTHORS   Kojima,T., Oh-eda,M., Hattori,K., Taniguchi,Y., Tamura,M., Ochi,N.
            and Yamaguchi,N.
  TITLE     Molecular cloning and expression of megakaryocyte potentiating
            factor cDNA
  JOURNAL   J Biol Chem 270 (37), 21984-21990 (1995)
   PUBMED   7665620
REFERENCE   10 (bases 1 to 2137)
  AUTHORS   Yamaguchi,N., Hattori,K., Oh-eda,M., Kojima,T., Imai,N. and Ochi,N.
  TITLE     A novel cytokine exhibiting megakaryocyte potentiating activity
            from a human pancreatic tumor cell line HPC-Y5
  JOURNAL   J Biol Chem 269 (2), 805-808 (1994)
   PUBMED   8288629
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL031258.12, AY743922.1,
            BC009272.2 and BC003512.1.
            
            On May 31, 2019 this sequence version replaced NM_001177355.2.
            
            Summary: This gene encodes a preproprotein that is proteolytically
            processed to generate two protein products, megakaryocyte
            potentiating factor and mesothelin. Megakaryocyte potentiating
            factor functions as a cytokine that can stimulate colony formation
            of bone marrow megakaryocytes. Mesothelin is a
            glycosylphosphatidylinositol-anchored cell-surface protein that may
            function as a cell adhesion protein. This protein is overexpressed
            in epithelial mesotheliomas, ovarian cancers and in specific
            squamous cell carcinomas. Alternative splicing results in multiple
            transcript variants, at least one of which encodes an isoform that
            is proteolytically processed. [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (3) differs in the 5' UTR,
            compared to variant 1. Both variants 1 and 3 encode the same
            isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC009272.2, SRR14038192.2892380.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AL031258.12        13669-13699
            32-32               AY743922.1         1-1
            33-1062             BC009272.2         1-1030
            1063-1065           AL031258.12        19022-19024
            1066-2134           BC009272.2         1034-2102
            2135-2137           BC003512.1         2410-2412
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..2137
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="mesothelin"
                     /db_xref="GeneID:10232"
                     /db_xref="HGNC:HGNC:7371"
                     /db_xref="MIM:601051"
     exon            1..88
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            89..130
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            131..224
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..2008
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 3; megakaryocyte potentiating factor; soluble MPF
                     mesothelin related protein; CAK1 antigen;
                     pre-pro-megakaryocyte-potentiating factor"
                     /codon_start=1
                     /product="mesothelin isoform 1 preproprotein"
                     /protein_id="NP_001170826.1"
                     /db_xref="CCDS:CCDS45370.1"
                     /db_xref="GeneID:10232"
                     /db_xref="HGNC:HGNC:7371"
                     /db_xref="MIM:601051"
                     /translation="MALPTARPLLGSCGTPALGSLLFLLFSLGWVQPSRTLAGETGQE
                     AAPLDGVLANPPNISSLSPRQLLGFPCAEVSGLSTERVRELAVALAQKNVKLSTEQLR
                     CLAHRLSEPPEDLDALPLDLLLFLNPDAFSGPQACTRFFSRITKANVDLLPRGAPERQ
                     RLLPAALACWGVRGSLLSEADVRALGGLACDLPGRFVAESAEVLLPRLVSCPGPLDQD
                     QQEAARAALQGGGPPYGPPSTWSVSTMDALRGLLPVLGQPIIRSIPQGIVAAWRQRSS
                     RDPSWRQPERTILRPRFRREVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLAT
                     QMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSL
                     ETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELS
                     SVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDL
                     KALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQ
                     RQDDLDTLGLGLQGGIPNGYLVLDLSMQEALSGTPCLLGPGPVLTVLALLLASTLA"
     exon            225..268
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            269..318
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            319..439
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            440..519
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            520..649
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            650..843
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            844..934
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1034
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1213
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1369
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1512
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..1640
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1735
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1736..1922
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2137
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2109..2114
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="hexamer: AGTAAA"
     polyA_site      2137
                     /gene="MSLN"
                     /gene_synonym="MPF; SMRP"
                     /note="major polyA site"
ORIGIN      
        1 gtcatttgtt ccctttgacg gcccgggagg ctgccaggct ctccaccccc acttcccaat
       61 tgaggaaacc gaggcagagg aggctcagag agctaccggt ggacccacgg tgcctccctc
      121 cctgggatct acacagacca tggccttgcc aacggctcga cccctgttgg ggtcctgtgg
      181 gacccccgcc ctcggcagcc tcctgttcct gctcttcagc ctcggatggg tgcagccctc
      241 gaggaccctg gctggagaga cagggcagga ggctgcgccc ctggacggag tcctggccaa
      301 cccacctaac atttccagcc tctcccctcg ccaactcctt ggcttcccgt gtgcggaggt
      361 gtccggcctg agcacggagc gtgtccggga gctggctgtg gccttggcac agaagaatgt
      421 caagctctca acagagcagc tgcgctgtct ggctcaccgg ctctctgagc cccccgagga
      481 cctggacgcc ctcccattgg acctgctgct attcctcaac ccagatgcgt tctcggggcc
      541 ccaggcctgc acccgtttct tctcccgcat cacgaaggcc aatgtggacc tgctcccgag
      601 gggggctccc gagcgacagc ggctgctgcc tgcggctctg gcctgctggg gtgtgcgggg
      661 gtctctgctg agcgaggctg atgtgcgggc tctgggaggc ctggcttgcg acctgcctgg
      721 gcgctttgtg gccgagtcgg ccgaagtgct gctaccccgg ctggtgagct gcccgggacc
      781 cctggaccag gaccagcagg aggcagccag ggcggctctg cagggcgggg gaccccccta
      841 cggccccccg tcgacatggt ctgtctccac gatggacgct ctgcggggcc tgctgcccgt
      901 gctgggccag cccatcatcc gcagcatccc gcagggcatc gtggccgcgt ggcggcaacg
      961 ctcctctcgg gacccatcct ggcggcagcc tgaacggacc atcctccggc cgcggttccg
     1021 gcgggaagtg gagaagacag cctgtccttc aggcaagaag gcccgcgaga tagacgagag
     1081 cctcatcttc tacaagaagt gggagctgga agcctgcgtg gatgcggccc tgctggccac
     1141 ccagatggac cgcgtgaacg ccatcccctt cacctacgag cagctggacg tcctaaagca
     1201 taaactggat gagctctacc cacaaggtta ccccgagtct gtgatccagc acctgggcta
     1261 cctcttcctc aagatgagcc ctgaggacat tcgcaagtgg aatgtgacgt ccctggagac
     1321 cctgaaggct ttgcttgaag tcaacaaagg gcacgaaatg agtcctcagg tggccaccct
     1381 gatcgaccgc tttgtgaagg gaaggggcca gctagacaaa gacaccctag acaccctgac
     1441 cgccttctac cctgggtacc tgtgctccct cagccccgag gagctgagct ccgtgccccc
     1501 cagcagcatc tgggcggtca ggccccagga cctggacacg tgtgacccaa ggcagctgga
     1561 cgtcctctat cccaaggccc gccttgcttt ccagaacatg aacgggtccg aatacttcgt
     1621 gaagatccag tccttcctgg gtggggcccc cacggaggat ttgaaggcgc tcagtcagca
     1681 gaatgtgagc atggacttgg ccacgttcat gaagctgcgg acggatgcgg tgctgccgtt
     1741 gactgtggct gaggtgcaga aacttctggg accccacgtg gagggcctga aggcggagga
     1801 gcggcaccgc ccggtgcggg actggatcct acggcagcgg caggacgacc tggacacgct
     1861 ggggctgggg ctacagggcg gcatccccaa cggctacctg gtcctagacc tcagcatgca
     1921 agaggccctc tcggggacgc cctgcctcct aggacctgga cctgttctca ccgtcctggc
     1981 actgctccta gcctccaccc tggcctgagg gccccactcc cttgctggcc ccagccctgc
     2041 tggggatccc cgcctggcca ggagcaggca cgggtggtcc ccgttccacc ccaagagaac
     2101 tcgcgctcag taaacgggaa catgccccct gcagaca
//
