LOCUS       NM_002439               4443 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens mutS homolog 3 (MSH3), mRNA.
ACCESSION   NM_002439
VERSION     NM_002439.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Singh,S., Singh,N., Gupta,P.S.S., Panda,S.K., Dhamija,I.,
            Nathiya,D., Kumar,S. and Sharma,S.
  TITLE     Assessing the impact of MSH3 and MSH6 polymorphisms on lung cancer
            risk in North Indian patients undergoing platinum chemotherapy
            through molecular dynamics simulation
  JOURNAL   Sci Rep 14 (1), 16164 (2024)
   PUBMED   39003369
  REMARK    GeneRIF: Assessing the impact of MSH3 and MSH6 polymorphisms on
            lung cancer risk in North Indian patients undergoing platinum
            chemotherapy through molecular dynamics simulation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Rashid,M., Rashid,R., Gadewal,N., Carethers,J.M., Koi,M., Brim,H.
            and Ashktorab,H.
  TITLE     High-throughput sequencing and in-silico analysis confirm
            pathogenicity of novel MSH3 variants in African American colorectal
            cancer
  JOURNAL   Neoplasia 49, 100970 (2024)
   PUBMED   38281411
  REMARK    GeneRIF: High-throughput sequencing and in-silico analysis confirm
            pathogenicity of novel MSH3 variants in African American colorectal
            cancer.
REFERENCE   3  (bases 1 to 4443)
  AUTHORS   Wang,X., Qu,Y., Xing,R., Zhou,J., Liu,Y., Zhang,H., Zhu,J., Ma,J.,
            Cui,X., Song,T., Xing,S., Ji,G., Liu,P., Sun,W., Fu,S. and Meng,X.
  TITLE     Novel insights into the ecDNA formation mechanism involving MSH3 in
            methotrexate-resistant human colorectal cancer cells
  JOURNAL   Int J Oncol 63 (6) (2023)
   PUBMED   37888748
  REMARK    GeneRIF: Novel insights into the ecDNA formation mechanism
            involving MSH3 in methotrexate-resistant human colorectal cancer
            cells.
REFERENCE   4  (bases 1 to 4443)
  AUTHORS   Villy,M.C., Masliah-Planchon,J., Schnitzler,A., Delhomelle,H.,
            Buecher,B., Filser,M., Merchadou,K., Golmard,L., Melaabi,S.,
            Vacher,S., Blanluet,M., Suybeng,V., Corsini,C., Dhooge,M.,
            Hamzaoui,N., Farelly,S., Ait Omar,A., Benamouzig,R., Caumette,V.,
            Bahuau,M., Cucherousset,J., Allory,Y., Stoppa-Lyonnet,D., Bieche,I.
            and Colas,C.
  TITLE     MSH3: a confirmed predisposing gene for adenomatous polyposis
  JOURNAL   J Med Genet 60 (12), 1198-1205 (2023)
   PUBMED   37402566
  REMARK    GeneRIF: MSH3: a confirmed predisposing gene for adenomatous
            polyposis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4443)
  AUTHORS   Oh,J.M., Kang,Y., Park,J., Sung,Y., Kim,D., Seo,Y., Lee,E.A.,
            Ra,J.S., Amarsanaa,E., Park,Y.U., Lee,S.Y., Hwang,J.M., Kim,H.,
            Scharer,O., Cho,S.W., Lee,C., Takata,K.I., Lee,J.Y. and Myung,K.
  TITLE     MSH2-MSH3 promotes DNA end resection during homologous
            recombination and blocks polymerase theta-mediated end-joining
            through interaction with SMARCAD1 and EXO1
  JOURNAL   Nucleic Acids Res 51 (11), 5584-5602 (2023)
   PUBMED   37140056
  REMARK    GeneRIF: MSH2-MSH3 promotes DNA end resection during homologous
            recombination and blocks polymerase theta-mediated end-joining
            through interaction with SMARCAD1 and EXO1.
REFERENCE   6  (bases 1 to 4443)
  AUTHORS   Risinger,J.I., Umar,A., Boyd,J., Berchuck,A., Kunkel,T.A. and
            Barrett,J.C.
  TITLE     Mutation of MSH3 in endometrial cancer and evidence for its
            functional role in heteroduplex repair
  JOURNAL   Nat Genet 14 (1), 102-105 (1996)
   PUBMED   8782829
REFERENCE   7  (bases 1 to 4443)
  AUTHORS   Watanabe,A., Ikejima,M., Suzuki,N. and Shimada,T.
  TITLE     Genomic organization and expression of the human MSH3 gene
  JOURNAL   Genomics 31 (3), 311-318 (1996)
   PUBMED   8838312
REFERENCE   8  (bases 1 to 4443)
  AUTHORS   Shinya,E. and Shimada,T.
  TITLE     Identification of two initiator elements in the bidirectional
            promoter of the human dihydrofolate reductase and mismatch repair
            protein 1 genes
  JOURNAL   Nucleic Acids Res 22 (11), 2143-2149 (1994)
   PUBMED   8029024
REFERENCE   9  (bases 1 to 4443)
  AUTHORS   Fujii,H. and Shimada,T.
  TITLE     Isolation and characterization of cDNA clones derived from the
            divergently transcribed gene in the region upstream from the human
            dihydrofolate reductase gene
  JOURNAL   J Biol Chem 264 (17), 10057-10064 (1989)
   PUBMED   2722860
REFERENCE   10 (bases 1 to 4443)
  AUTHORS   Chen,M.J., Shimada,T., Moulton,A.D., Cline,A., Humphries,R.K.,
            Maizel,J. and Nienhuis,A.W.
  TITLE     The functional human dihydrofolate reductase gene
  JOURNAL   J Biol Chem 259 (6), 3933-3943 (1984)
   PUBMED   6323448
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC130434.1, J04810.1,
            AC022493.12, U61981.1 and AA601983.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_002439.4.
            
            Summary: The protein encoded by this gene forms a heterodimer with
            MSH2 to form MutS beta, part of the post-replicative DNA mismatch
            repair system. MutS beta initiates mismatch repair by binding to a
            mismatch and then forming a complex with MutL alpha heterodimer.
            This gene contains a polymorphic 9 bp tandem repeat sequence in the
            first exon. The repeat is present 6 times in the reference genome
            sequence and 3-7 repeats have been reported. Defects in this gene
            are a cause of susceptibility to endometrial cancer. [provided by
            RefSeq, Mar 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U61981.1, SRR1803615.35610.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265081.7/ ENSP00000265081.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BC130434.1         5-19
            16-310              J04810.1           1-295
            311-2921            BC130434.1         315-2925
            2922-2922           AC022493.12        30479-30479         c
            2923-4262           U61981.1           2836-4175
            4263-4414           AA601983.1         1-152               c
            4415-4443           U61981.1           4321-4349
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q14.1"
     gene            1..4443
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /note="mutS homolog 3"
                     /db_xref="GeneID:4437"
                     /db_xref="HGNC:HGNC:7326"
                     /db_xref="MIM:600887"
     exon            1..313
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..3490
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /note="mismatch repair protein 1; divergent upstream
                     protein; hMSH3; epididymis secretory sperm binding
                     protein"
                     /codon_start=1
                     /product="DNA mismatch repair protein Msh3"
                     /protein_id="NP_002430.3"
                     /db_xref="CCDS:CCDS34195.1"
                     /db_xref="GeneID:4437"
                     /db_xref="HGNC:HGNC:7326"
                     /db_xref="MIM:600887"
                     /translation="MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAA
                     DQVDPGAAAAAAAAAAAAPPAPPAPAFPPQLPPHIATEIDRRKKRPLENDGPVKKKVK
                     KVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEFCCDSALPQSRVQTESLQERF
                     AVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSHENLQKTAS
                     KSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHL
                     DHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALY
                     TKSTLIGEDVNPLIKLDDAVNVDEIMTDTSTSYLLCISENKENVRDKKKGNIFIGIVG
                     VQPATGEVVFDSFQDSASRSELETRMSSLQPVELLLPSALSEQTEALIHRATSVSVQD
                     DRIRVERMDNIYFEYSHAFQAVTEFYAKDTVDIKGSQIISGIVNLEKPVICSLAAIIK
                     YLKEFNLEKMLSKPENFKQLSSKMEFMTINGTTLRNLEILQNQTDMKTKGSLLWVLDH
                     TKTSFGRRKLKKWVTQPLLKLREINARLDAVSEVLHSESSVFGQIENHLRKLPDIERG
                     LCSIYHKKCSTQEFFLIVKTLYHLKSEFQAIIPAVNSHIQSDLLRTVILEIPELLSPV
                     EHYLKILNEQAAKVGDKTELFKDLSDFPLIKKRKDEIQGVIDEIRMHLQEIRKILKNP
                     SAQYVTVSGQEFMIEIKNSAVSCIPTDWVKVGSTKAVSRFHSPFIVENYRHLNQLREQ
                     LVLDCSAEWLDFLEKFSEHYHSLCKAVHHLATVDCIFSLAKVAKQGDYCRPTVQEERK
                     IVIKNGRHPVIDVLLGEQDQYVPNNTDLSEDSERVMIITGPNMGGKSSYIKQVALITI
                     MAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGQSTFMEELTDTAEIIRKATSQSLV
                     ILDELGRGTSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKNYSHQVGNYHM
                     GFLVSEDESKLDPGAAEQVPDFVTFLYQITRGIAARSYGLNVAKLADVPGEILKKAAH
                     KSKELEGLINTKRKRLKYFAKLWTMHNAQDLQKWTEEFNMEETQTSLLH"
     exon            314..434
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            435..655
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            656..868
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            869..985
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1103
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1249
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1416
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1529
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1644
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1729
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1839
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1972
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2160
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2329
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2394
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2511
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2512..2619
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2731
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2889
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..3076
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3206
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3207..3378
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     exon            3379..4443
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3525..3530
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /note="hexamer: ATTAAA"
     polyA_site      3551
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /note="major polyA site"
     regulatory      4416..4421
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /note="hexamer: AATAAA"
     polyA_site      4443
                     /gene="MSH3"
                     /gene_synonym="DUP; FAP4; MRP1"
                     /note="major polyA site"
ORIGIN      
        1 agacgcctgg gaactgcggc cgcgggctcg cgctcctcgc caggccctgc cgccgggctg
       61 ccatccttgc cctgccatgt ctcgccggaa gcctgcgtcg ggcggcctcg ctgcctccag
      121 ctcagcccct gcgaggcaag cggttttgag ccgattcttc cagtctacgg gaagcctgaa
      181 atccacctcc tcctccacag gtgcagccga ccaggtggac cctggcgctg cagcggctgc
      241 agcggccgca gcggccgcag cgcccccagc gcccccagct cccgccttcc cgccccagct
      301 gccgccgcac atagctacag aaattgacag aagaaagaag agaccattgg aaaatgatgg
      361 gcctgttaaa aagaaagtaa agaaagtcca acaaaaggaa ggaggaagtg atctgggaat
      421 gtctggcaac tctgagccaa agaaatgtct gaggaccagg aatgtttcaa agtctctgga
      481 aaaattgaaa gaattctgct gcgattctgc ccttcctcaa agtagagtcc agacagaatc
      541 tctgcaggag agatttgcag ttctgccaaa atgtactgat tttgatgata tcagtcttct
      601 acacgcaaag aatgcagttt cttctgaaga ttcgaaacgt caaattaatc aaaaggacac
      661 aacacttttt gatctcagtc agtttggatc atcaaataca agtcatgaaa atttacagaa
      721 aactgcttcc aaatcagcta acaaacggtc caaaagcatc tatacgccgc tagaattaca
      781 atacatagaa atgaagcagc agcacaaaga tgcagttttg tgtgtggaat gtggatataa
      841 gtatagattc tttggggaag atgcagagat tgcagcccga gagctcaata tttattgcca
      901 tttagatcac aactttatga cagcaagtat acctactcac agactgtttg ttcatgtacg
      961 ccgcctggtg gcaaaaggat ataaggtggg agttgtgaag caaactgaaa ctgcagcatt
     1021 aaaggccatt ggagacaaca gaagttcact cttttcccgg aaattgactg ccctttatac
     1081 aaaatctaca cttattggag aagatgtgaa tcccctaatc aagctggatg atgctgtaaa
     1141 tgttgatgag ataatgactg atacttctac cagctatctt ctgtgcatct ctgaaaataa
     1201 ggaaaatgtt agggacaaaa aaaagggcaa catttttatt ggcattgtgg gagtgcagcc
     1261 tgccacaggc gaggttgtgt ttgatagttt ccaggactct gcttctcgtt cagagctaga
     1321 aacccggatg tcaagcctgc agccagtaga gctgctgctt ccttcggcct tgtccgagca
     1381 aacagaggcg ctcatccaca gagccacatc tgttagtgtg caggatgaca gaattcgagt
     1441 cgaaaggatg gataacattt attttgaata cagccatgct ttccaggcag ttacagagtt
     1501 ttatgcaaaa gatacagttg acatcaaagg ttctcaaatt atttctggca ttgttaactt
     1561 agagaagcct gtgatttgct ctttggctgc catcataaaa tacctcaaag aattcaactt
     1621 ggaaaagatg ctctccaaac ctgagaattt taaacagcta tcaagtaaaa tggaatttat
     1681 gacaattaat ggaacaacat taaggaatct ggaaatccta cagaatcaga ctgatatgaa
     1741 aaccaaagga agtttgctgt gggttttaga ccacactaaa acttcatttg ggagacggaa
     1801 gttaaagaag tgggtgaccc agccactcct taaattaagg gaaataaatg cccggcttga
     1861 tgctgtatcg gaagttctcc attcagaatc tagtgtgttt ggtcagatag aaaatcatct
     1921 acgtaaattg cccgacatag agaggggact ctgtagcatt tatcacaaaa aatgttctac
     1981 ccaagagttc ttcttgattg tcaaaacttt atatcaccta aagtcagaat ttcaagcaat
     2041 aatacctgct gttaattccc acattcagtc agacttgctc cggaccgtta ttttagaaat
     2101 tcctgaactc ctcagtccag tggagcatta cttaaagata ctcaatgaac aagctgccaa
     2161 agttggggat aaaactgaat tatttaaaga cctttctgac ttccctttaa taaaaaagag
     2221 gaaggatgaa attcaaggtg ttattgacga gatccgaatg catttgcaag aaatacgaaa
     2281 aatactaaaa aatccttctg cacaatatgt gacagtatca ggacaggagt ttatgataga
     2341 aataaagaac tctgctgtat cttgtatacc aactgattgg gtaaaggttg gaagcacaaa
     2401 agctgtgagc cgctttcact ctccttttat tgtagaaaat tacagacatc tgaatcagct
     2461 ccgggagcag ctagtccttg actgcagtgc tgaatggctt gattttctag agaaattcag
     2521 tgaacattat cactccttgt gtaaagcagt gcatcaccta gcaactgttg actgcatttt
     2581 ctccctggcc aaggtcgcta agcaaggaga ttactgcaga ccaactgtac aagaagaaag
     2641 aaaaattgta ataaaaaatg gaaggcaccc tgtgattgat gtgttgctgg gagaacagga
     2701 tcaatatgtc ccaaataata cagatttatc agaggactca gagagagtaa tgataattac
     2761 cggaccaaac atgggtggaa agagctccta cataaaacaa gttgcattga ttaccatcat
     2821 ggctcagatt ggctcctatg ttcctgcaga agaagcgaca attgggattg tggatggcat
     2881 tttcacaagg atgggtgctg cagacaatat atataaagga cagagtacat ttatggaaga
     2941 actgactgac acagcagaaa taatcagaaa agcaacatca cagtccttgg ttatcttgga
     3001 tgaactagga agagggacga gcactcatga tggaattgcc attgcctatg ctacacttga
     3061 gtatttcatc agagatgtga aatccttaac cctgtttgtc acccattatc cgccagtttg
     3121 tgaactagaa aaaaattact cacaccaggt ggggaattac cacatgggat tcttggtcag
     3181 tgaggatgaa agcaaactgg atccaggcgc agcagaacaa gtccctgatt ttgtcacctt
     3241 cctttaccaa ataactagag gaattgcagc aaggagttat ggattaaatg tggctaaact
     3301 agcagatgtt cctggagaaa ttttgaagaa agcagctcac aagtcaaaag agctggaagg
     3361 attaataaat acgaaaagaa agagactcaa gtattttgca aagttatgga cgatgcataa
     3421 tgcacaagac ctgcagaagt ggacagagga gttcaacatg gaagaaacac agacttctct
     3481 tcttcattaa aatgaagact acatttgtga acaaaaaatg gagaattaaa aataccaact
     3541 gtacaaaata actctccagt aacagcctat ctttgtgtga catgtgagca taaaattatg
     3601 accatggtat attcctattg gaaacagaga ggtttttctg aagacagtct ttttcaagtt
     3661 tctgtcttcc taacttttct acgtataaac actcttgaat agacttccac tttgtaatta
     3721 gaaaatttta tggacagtaa gtccagtaaa gccttaagtg gcagaatata attcccaagc
     3781 ttttggaggg tgatataaaa atttacttga tatttttatt tgtttcagtt cagataattg
     3841 gcaactgggt gaatctggca ggaatctatc cattgaacta aaataatttt attatgcaac
     3901 cagtttatcc accaagaaca taagaatttt ttataagtag aaagaattgg ccaggcatgg
     3961 tggctcatgc ctgtaatccc agcactttgg gaggccaagg taggcagatc acctgaggtc
     4021 aggagttcaa gaccagcctg gccaacatgg caaaacccca tctttactaa aaatataaag
     4081 tacatctcta ctaaaaatac gaaaaaatta gctgggcatg gtggcgcaca cctgtagtcc
     4141 cagctactcc ggaggctgag gcaggagaat ctcttgaacc tgggaggcgg aggttgcaat
     4201 gagccgagat cacgtcactg cactccagct tgggcaacag agcaagactc catctcaaaa
     4261 aaaaaaaaag aaaaaagaaa agaaatagaa ttatcaagct tttaaaaact agagcacaga
     4321 aggaataagg tcatgaaatt taaaaggtta aatattgtca taggattaag cagtttaaag
     4381 attgttggat gaaattattt gtcattcatt caagtaataa atatttaatg aatacttgct
     4441 ata
//
