LOCUS       NM_001406652           10254 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens mutS homolog 2 (MSH2), transcript variant 24, mRNA.
ACCESSION   NM_001406652
VERSION     NM_001406652.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Tu,X., Zhang,H. and Ren,H.
  TITLE     LINC01232 targeting miR-1250-3p/MSH2 axis attenuates mesangial cell
            proliferation and fibrosis in diabetic nephropathy
  JOURNAL   Mol Cell Biochem 479 (8), 2093-2103 (2024)
   PUBMED   37642881
  REMARK    GeneRIF: LINC01232 targeting miR-1250-3p/MSH2 axis attenuates
            mesangial cell proliferation and fibrosis in diabetic nephropathy.
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Li,M., Yan,X., Liu,H., Miao,W., Wu,W., Zhao,Y., Wang,C. and Liu,H.
  TITLE     Novel MSH2 and TSC2 variants in a Chinese family with Lynch
            syndrome and their synergistic impact in urothelial carcinoma
  JOURNAL   Transl Res 265, 26-35 (2024)
   PUBMED   37914149
  REMARK    GeneRIF: Novel MSH2 and TSC2 variants in a Chinese family with
            Lynch syndrome and their synergistic impact in urothelial
            carcinoma.
REFERENCE   3  (bases 1 to 10254)
  AUTHORS   Mirabdolhosseini,S.M., Yaghoob Taleghani,M., Rejali,L., Sadeghi,H.,
            Fatemi,N., Tavallaei,M., Famil Meyari,A., Saeidi,N., Ketabi
            Moghadam,P., Sadeghi,A., Asadzadeh Aghdaei,H., Zali,M.R. and
            Nazemalhosseini Mojarad,E.
  TITLE     Rare single-nucleotide variants of MLH1 and MSH2 genes in patients
            with Lynch syndrome
  JOURNAL   Cancer Rep (Hoboken) 7 (1), e1930 (2024)
   PUBMED   37919876
  REMARK    GeneRIF: Rare single-nucleotide variants of MLH1 and MSH2 genes in
            patients with Lynch syndrome.
REFERENCE   4  (bases 1 to 10254)
  AUTHORS   Sharbati,F., Tabriz,H.M. and Nazar,E.
  TITLE     Assessing the role of MSH2 and MSH6 gene expression deficiency in
            prostate cancer progression, a cross-sectional study
  JOURNAL   Cancer Treat Res Commun 40, 100826 (2024)
   PUBMED   38870667
  REMARK    GeneRIF: Assessing the role of MSH2 and MSH6 gene expression
            deficiency in prostate cancer progression, a cross-sectional study.
REFERENCE   5  (bases 1 to 10254)
  AUTHORS   Dutta,P., Pal,D., Roy,A., Mandal,R.K. and Panda,C.K.
  TITLE     Role of MLH1 and MSH2 deficiency in the development of
            tumorigenesis and chemo-tolerance of cervical Carcinoma: Clinical
            implications
  JOURNAL   Gene 888, 147746 (2023)
   PUBMED   37657688
  REMARK    GeneRIF: Role of MLH1 and MSH2 deficiency in the development of
            tumorigenesis and chemo-tolerance of cervical Carcinoma: Clinical
            implications.
REFERENCE   6  (bases 1 to 10254)
  AUTHORS   Wilson,T.M., Ewel,A., Duguid,J.R., Eble,J.N., Lescoe,M.K.,
            Fishel,R. and Kelley,M.R.
  TITLE     Differential cellular expression of the human MSH2 repair enzyme in
            small and large intestine
  JOURNAL   Cancer Res 55 (22), 5146-5150 (1995)
   PUBMED   7585562
REFERENCE   7  (bases 1 to 10254)
  AUTHORS   Wei,Q., Xu,X., Cheng,L., Legerski,R.J. and Ali-Osman,F.
  TITLE     Simultaneous amplification of four DNA repair genes and beta-actin
            in human lymphocytes by multiplex reverse transcriptase-PCR
  JOURNAL   Cancer Res 55 (21), 5025-5029 (1995)
   PUBMED   7585546
REFERENCE   8  (bases 1 to 10254)
  AUTHORS   Drummond,J.T., Li,G.M., Longley,M.J. and Modrich,P.
  TITLE     Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch
            repair to tumor cells
  JOURNAL   Science 268 (5219), 1909-1912 (1995)
   PUBMED   7604264
REFERENCE   9  (bases 1 to 10254)
  AUTHORS   Idos,G. and Valle,L.
  TITLE     Lynch Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301390
REFERENCE   10 (bases 1 to 10254)
  AUTHORS   Lynch,H.T., Schuelke,G.S., Kimberling,W.J., Albano,W.A.,
            Lynch,J.F., Biscone,K.A., Lipkin,M.L., Deschner,E.E., Mikol,Y.B.,
            Sandberg,A.A. et al.
  TITLE     Hereditary nonpolyposis colorectal cancer (Lynch syndromes I and
            II). II. Biomarker studies
  JOURNAL   Cancer 56 (4), 939-951 (1985)
   PUBMED   4016686
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC079775.6 and AC138655.1.
            
            Summary: This locus is frequently mutated in hereditary
            nonpolyposis colon cancer (HNPCC). When cloned, it was discovered
            to be a human homolog of the E. coli mismatch repair gene mutS,
            consistent with the characteristic alterations in microsatellite
            sequences (RER+ phenotype) found in HNPCC. Two transcript variants
            encoding different isoforms have been found for this gene.
            [provided by RefSeq, Apr 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.682517.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-247               AC079775.6         98145-98391
            248-402             AC079775.6         103390-103544
            403-681             AC079775.6         105083-105361
            682-828             AC079775.6         107403-107549
            829-962             AC079775.6         111285-111418
            963-1162            AC079775.6         124731-124930
            1163-1272           AC079775.6         140537-140646
            1273-1396           AC079775.6         158020-158143
            1397-1547           AC079775.6         161647-161797
            1548-1645           AC079775.6         165954-166051
            1646-1891           AC138655.1         27-272
            1892-2096           AC138655.1         1369-1573
            2097-2344           AC138655.1         3274-3521
            2345-2520           AC138655.1         5698-5873
            2521-10254          AC138655.1         37305-45038
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p21-p16.3"
     gene            1..10254
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /note="mutS homolog 2"
                     /db_xref="GeneID:4436"
                     /db_xref="HGNC:HGNC:7325"
                     /db_xref="MIM:609309"
     exon            1..247
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..2652
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /note="isoform 21 is encoded by transcript variant 24;
                     mutS homolog 2, colon cancer, nonpolyposis type 1; DNA
                     mismatch repair protein Msh2 transcript; MutS-like 2"
                     /codon_start=1
                     /product="DNA mismatch repair protein Msh2 isoform 21"
                     /protein_id="NP_001393581.1"
                     /db_xref="GeneID:4436"
                     /db_xref="HGNC:HGNC:7325"
                     /db_xref="MIM:609309"
                     /translation="MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYT
                     AHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEV
                     YKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQ
                     VGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQ
                     RGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQGSVEDTTGSQSL
                     AALLNKCKTPQGQRLVNQWIKQPLMDKNRIEERLNLVEAFVEDAELRQTLQEDLLRRF
                     PDLNRLAKKFQRQAANLQDCYRLYQGINQLPNVIQALEKHEGKHQKLLLAVFVTPLTD
                     LRSDFSKFQEMIETTLDMDQVENHEFLVKPSFDPNLSELREIMNDLEKKMQSTLISAA
                     RDLGLDPGKQIKLDSSAQFGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSKLTS
                     LNEEYTKNKTEYEEAQDAIVKEIVNISSGYVEPMQTLNDVLAQLDAVVSFAHVSNGAP
                     VPYVRPAILEKGQGRIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGG
                     KSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVGAGDSQLKGVSTFMAEMLET
                     ASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFCMFATHFHELTAL
                     ANQIPTVNNLHVTALTTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHVIECAKQKA
                     LELEEFQYIGESQGYDIMEPAAKKCYLERENLRVTEPKDQCLILLTWKRKLRGGKRSA
                     CSRPERQNQGSGELS"
     exon            248..402
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            403..681
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            682..828
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            829..962
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1162
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1272
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1396
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1547
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1548..1645
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1891
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1892..2096
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2344
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2520
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2521..10254
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2774..2779
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /note="hexamer: AATATA"
     polyA_site      2795
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /note="major polyA site"
     regulatory      5795..5800
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /note="hexamer: GATAAA"
     polyA_site      5814
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
     regulatory      8489..8494
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /note="hexamer: AATACA"
     polyA_site      8508
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
     regulatory      10228..10233
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
                     /note="hexamer: AATAAA"
     polyA_site      10248
                     /gene="MSH2"
                     /gene_synonym="COCA1; FCC1; hMSH2; HNPCC; HNPCC1; LCFS2;
                     LYNCH1; MMRCS2; MSH-2"
ORIGIN      
        1 gcattttctt caaccaggag gtgaggaggt ttcgacatgg cggtgcagcc gaaggagacg
       61 ctgcagttgg agagcgcggc cgaggtcggc ttcgtgcgct tctttcaggg catgccggag
      121 aagccgacca ccacagtgcg ccttttcgac cggggcgact tctatacggc gcacggcgag
      181 gacgcgctgc tggccgcccg ggaggtgttc aagacccagg gggtgatcaa gtacatgggg
      241 ccggcaggag caaagaatct gcagagtgtt gtgcttagta aaatgaattt tgaatctttt
      301 gtaaaagatc ttcttctggt tcgtcagtat agagttgaag tttataagaa tagagctgga
      361 aataaggcat ccaaggagaa tgattggtat ttggcatata aggcttctcc tggcaatctc
      421 tctcagtttg aagacattct ctttggtaac aatgatatgt cagcttccat tggtgttgtg
      481 ggtgttaaaa tgtccgcagt tgatggccag agacaggttg gagttgggta tgtggattcc
      541 atacagagga aactaggact gtgtgaattc cctgataatg atcagttctc caatcttgag
      601 gctctcctca tccagattgg accaaaggaa tgtgttttac ccggaggaga gactgctgga
      661 gacatgggga aactgagaca gataattcaa agaggaggaa ttctgatcac agaaagaaaa
      721 aaagctgact tttccacaaa agacatttat caggacctca accggttgtt gaaaggcaaa
      781 aagggagagc agatgaatag tgctgtattg ccagaaatgg agaatcaggg ttctgttgaa
      841 gataccactg gctctcagtc tctggctgcc ttgctgaata agtgtaaaac ccctcaagga
      901 caaagacttg ttaaccagtg gattaagcag cctctcatgg ataagaacag aatagaggag
      961 agattgaatt tagtggaagc ttttgtagaa gatgcagaat tgaggcagac tttacaagaa
     1021 gatttacttc gtcgattccc agatcttaac cgacttgcca agaagtttca aagacaagca
     1081 gcaaacttac aagattgtta ccgactctat cagggtataa atcaactacc taatgttata
     1141 caggctctgg aaaaacatga aggaaaacac cagaaattat tgttggcagt ttttgtgact
     1201 cctcttactg atcttcgttc tgacttctcc aagtttcagg aaatgataga aacaacttta
     1261 gatatggatc aggtggaaaa ccatgaattc cttgtaaaac cttcatttga tcctaatctc
     1321 agtgaattaa gagaaataat gaatgacttg gaaaagaaga tgcagtcaac attaataagt
     1381 gcagccagag atcttggctt ggaccctggc aaacagatta aactggattc cagtgcacag
     1441 tttggatatt actttcgtgt aacctgtaag gaagaaaaag tccttcgtaa caataaaaac
     1501 tttagtactg tagatatcca gaagaatggt gttaaattta ccaacagcaa attgacttct
     1561 ttaaatgaag agtataccaa aaataaaaca gaatatgaag aagcccagga tgccattgtt
     1621 aaagaaattg tcaatatttc ttcaggctat gtagaaccaa tgcagacact caatgatgtg
     1681 ttagctcagc tagatgctgt tgtcagcttt gctcacgtgt caaatggagc acctgttcca
     1741 tatgtacgac cagccatttt ggagaaagga caaggaagaa ttatattaaa agcatccagg
     1801 catgcttgtg ttgaagttca agatgaaatt gcatttattc ctaatgacgt atactttgaa
     1861 aaagataaac agatgttcca catcattact ggccccaata tgggaggtaa atcaacatat
     1921 attcgacaaa ctggggtgat agtactcatg gcccaaattg ggtgttttgt gccatgtgag
     1981 tcagcagaag tgtccattgt ggactgcatc ttagcccgag taggggctgg tgacagtcaa
     2041 ttgaaaggag tctccacgtt catggctgaa atgttggaaa ctgcttctat cctcaggtct
     2101 gcaaccaaag attcattaat aatcatagat gaattgggaa gaggaacttc tacctacgat
     2161 ggatttgggt tagcatgggc tatatcagaa tacattgcaa caaagattgg tgctttttgc
     2221 atgtttgcaa cccattttca tgaacttact gccttggcca atcagatacc aactgttaat
     2281 aatctacatg tcacagcact caccactgaa gagaccttaa ctatgcttta tcaggtgaag
     2341 aaaggtgtct gtgatcaaag ttttgggatt catgttgcag agcttgctaa tttccctaag
     2401 catgtaatag agtgtgctaa acagaaagcc ctggaacttg aggagtttca gtatattgga
     2461 gaatcgcaag gatatgatat catggaacca gcagcaaaga agtgctatct ggaaagagag
     2521 aacctcagag tgacagagcc aaaagaccag tgcctcattt tgctgacatg gaaaaggaaa
     2581 cttcgtgggg gaaagagatc tgcttgcagt cggccagaga gacagaacca gggcagtggt
     2641 gagctctcat gacctggtgt ctgttgcctt ctggttaagt ttttcatttg taattctaca
     2701 aacatccctt ctgtaaacat ttccctcaaa atggagcagg aagctctcaa aaatggacca
     2761 gaaaggggtc aggaatataa ctttctctgc ccagattcca ggacttacag tgagaaagcg
     2821 ccttctggga acttcacaat ggctaaagtg tgctaatggg atgatgtgcc cttgtacacc
     2881 cactgcctct gaactctgct ctgcattgct gagcaaacta catttcccag aactccttgt
     2941 tggattcctt ccaaacaggt ttaccactgg gagagcctgt tggttgggga gggcaggaag
     3001 agggaggaaa gaggaaggga ctcacttcct gtttccagct gaagtctaaa tcaatccact
     3061 atcaacaggt agctatcata ctaccctcat tgtcacccct cagaggtccc actgcagctg
     3121 cataatgtcc cctcagtggc ctgaacatga gatgaacaac actcttcttg ggagtaccag
     3181 ccttgcttgg ttcatggcca cttttcctga ttatcttgca gctatattag gtcatgtgac
     3241 aaagttctgg ccagtggcaa gggaacacaa gtgataggta cagatagaag tgtctgatac
     3301 tacatagatt atgcttgcac tcactcttaa gagagagaca tgaactttta ccaacggaag
     3361 ccagtattat tttgaacctc tgttagagtg gcttgaatct gtatcctaac ttgtatccct
     3421 aatgtgtgac ccatgaaaat tagccaggca gcaccagttc caaagaagct cacactcccc
     3481 tgcggctgct tctgccaagg tcactgatat ttccctttgc taaatcttgt gggtgttttc
     3541 ttcagtcctt gtcttaatca ctcagtggca cttggcactt attccttctt gaaacccttg
     3601 tttcccttgg ctttgtggca tcctgtgctc ttggttttct cccatatctc tgaccctctt
     3661 tccttagtct tttttcttct tcctcctgtc ccttaaatgc tggttgtgat cctcttttta
     3721 tctcattcta cacactcaca gcctgagtaa ttcacaccat cttgatgctg agaacttcca
     3781 aaatgttggt ctagcctggg tcattgttat gagctctaga ctcacaaggc caattgcttg
     3841 gtgggaaccc ctcccccatg gttatctcat gggtccctga agtccaactt ctccttcatt
     3901 gaactcatca cctcttctgt tcctcctcct gggttcccag gctcagtggt ggcaccactg
     3961 tctacctggc tgcttagcct gagacctggc tccgtcccaa ttcctctctc tcagtcttat
     4021 catccccatc caggcaaatc attgattctg tggacctact ctttcgggtg tccctcaaat
     4081 ctctccacgt ctctgtgttc tcactagcac taccttggtc caccctgcca tctgctttcc
     4141 tcctccactc ctgcattctg agtcattttc ggcagcacac gcatccttaa aacccctcca
     4201 ctggcttgcc agtgtcctca ggattaggcg aaaagtcttt gctttgtttt acaaggccct
     4261 tcgctatctg gccccctcat tacctccctt gctctgcatg ctccagtcct gcagaactac
     4321 acacagttcc cccaacaagg ccctgctctg ttcttcccac acactgctcc tctgcctggg
     4381 ccactcttcc tgctccttgt cagcaggctt gctgctctca ggctcagcat ggacagctgc
     4441 ttctgagagc cttctctgcc tacccaggct gggtggctgc ctctctttgg tgtgcccatg
     4501 gcagcccaga atgcctggtg gacagggagc cctcagcagg ccgtactgca gcgccctgcc
     4561 cccgtcagcc tccaggagcc tggagtccag ggacatcaag ggcggtcctg tctttctcac
     4621 ccttgtctct ccagccccta acacagggga tgcctgaccc caaactagac gagttacttg
     4681 acctctctga cccaagacaa aatgggagga aagtgccaaa tttccaagat tggccagggg
     4741 attaaataag ataaatatgc aagtctctta tctgggggtc tggcttggta aatataaagt
     4801 tcttttttct tttcttcctt tttctttttt ttttttcttt ctttttgaga cagggtctta
     4861 ctctgtcacc aaggctggag tgcagtggca tgatcatggc tcaatgaaac atcgacttcc
     4921 tgggctcagg cgatcctccc acctcagccc cctgagtctc ttggactcca ggcgtgcacc
     4981 accatgactg gctaattttt tgtattttta gtaaagacag ggtttcgaca tgttgcctag
     5041 gctggtctcg aactcctagg ctaaagtgat ccacttgtct cagcctccca aagtgctggg
     5101 attatagaca tgagccacca tgcccagcta aaagttcctt tttaaaatct gcttgttaga
     5161 tacactcata gaaaggtaac tggccacaga agggagagga atggcagtcc atccagggat
     5221 cactggagtg tcatatgaaa tgttatagga atcacaggcc ttagaacttg aaaggaaccc
     5281 aaggatcatc taggctactt tatgcaggta aaacagccac ctgtgcccat cacatagctg
     5341 gggcacagct ggagacccca acagagagga gagctgatgg gtgacgagaa atcaggcctc
     5401 tccgccacgg cagcctagct aatgggtctt ggctggaagc taacaggaag gcctctttcc
     5461 agaaacactg taagccagtg tttctcagat tgctgggtgt aattcatagg cagatcatga
     5521 aatcagttta atagctttga ccagcattaa cctatttatg cctagcgttc ccttattgga
     5581 acactaagtc tgtgagagtt atttacatcc tactgcttaa ggtcatcgcc aaaatctgat
     5641 tttttacaca aaaaatttgc aacctccagc ataaatgggt taaaacaaga caaaacaaaa
     5701 caataccaga atggaaaata gtgcatgatc tgtacagtat agttgtagaa aacttcttgt
     5761 tttatcattt gatgtcatga aagtccctgc tgtagataaa agatggagct tgtgcttctg
     5821 agtggtcatg ctcaacaggg tggggagccc aggggagtgg ggagtgatcg tatagacaga
     5881 ggtgggtggg gccagtgtga gcctgatggt caattacttc tcatttctag ggaaaattga
     5941 aggaaaagaa ggagggggat gtggagggga gagaaggcct cagtagagtt tgcactatta
     6001 ttagggcaag taagctgctt ctgaaaagaa ggggtttgca aagccaaccc aggcaaaagc
     6061 aatctgctgg aagaacttca tccccagctg acactgtggg aaggacccca tgcagaagca
     6121 atagggcagc ctggtcccat atcctcatga aatgcctctt ataattgtga catcttgcaa
     6181 ttgtggagga ctttacactt ttcggagttc ctagcccctc acttatttct cgtaagaccg
     6241 ctgggaggtg gggggatggt atcatcatcc cactttagag atgaggaaac aggatcagag
     6301 tgagctaaat gactgccaga tccaaaacta gaattcagac ctcctagttt ctaagtggac
     6361 gctctttcta caccaccata atgtgagtgt tctgtgttta cagggtgtat tcaagtccat
     6421 gactgcccat tagaatcccc ccaaaaaatt ccaggactgg cctgagttgc tccttagacc
     6481 aatgaaatca gactcctggg agtacggccc gggcctcggg atcctttaaa gctccatttg
     6541 gagagcctcg ggcacagcca ggttggatcc atctcccagt cccccagcct tggctcagcc
     6601 tggccaagct gcccaggagg tcccttggtg ccctgggctc tgtttcactg ttgttttgta
     6661 gagcaacttc ccagtgatgc tgccactggg ccccatccta acagtgaagt cccccgggcc
     6721 ctcctgagag gaggtgtgaa ctggaagatg gggaggcagg cggctctgac agacagaaag
     6781 caaacagctc agaggggtgg caggctgcat tttattcatc gttaatttaa acacccttca
     6841 agtcctctct tggaatgctg ctcagaaaaa tagatgtatt gtttgagaaa ccctgcaggc
     6901 ttgtcccgca tgctctagcc ccctcctgag agaacagata gcataaaaaa tgatttgtaa
     6961 agcaaggggg agcttcctta gggaagaagg ggaaggggaa gagggtttgg ggccaggtcc
     7021 gagtgcagaa atcctcaatg catgagacta gcgtggaagg tgtagcaatt gtgctctggg
     7081 gtgcctgaaa gtgccagagc tgcttcaggg gcaagagtcc aggccccaag tccatgctga
     7141 tgagcccacc ctgggggtca ggaatggcct cagcaggccc tccctccctc cctctccacc
     7201 ctacaaagtg aggagccttg agtcaccacc agcacattat acaacaatac aagaaccctg
     7261 caacagataa agccccagcg cctcttctgg actcagatgc cctaggctgg ctgtctggct
     7321 gtgctttcca gacagtgtgt atgtggaatt gtgctttttg ttttttaaga atgtaaaaag
     7381 ttacagtaag atcgaaccac agggcccgtc gctcctatgg tctctgcctg actgggctgc
     7441 cgtctgcctc agttccccag aagcttctcc tttggccatg agggctcagt catccctcac
     7501 cccagagtcc acaggaagag ggggtctgct gggaggcctg tctgaaggac ggaggatcct
     7561 gggtcaattt agcagctatt ttccagggtt tggcttgggt ttggatgctg gcttctgtgt
     7621 gaaacctgaa tacatgcaaa ttgtacataa aactccccca aggcagagag ggattttcca
     7681 ggccctggta catctctaga gagttaaaaa tgggaaatct ttcttcttaa agtggcccag
     7741 actgagactt ttccttgggg aaaagggtta gtagctcttt gtaaggctgg tgtgtatgtg
     7801 tgtgtgtata tatatataca tatatgcatg atgctgtgca aatgcccagg gctgtctggc
     7861 attttccaca aaatgagagc ctgagattgc ctaagccttc tgatgccttc tccaggcctg
     7921 gaggcactgc ttcattcaga ggacacaaag gcctgaccac ctggctttag caagctagga
     7981 cacccagggt ggcttcttta cctttctcct cagctctgag aaggctgcta gccaagactc
     8041 tggattctct gtggccacag tcatatggtg agggcctctt ggagttcatt caaactttaa
     8101 gggagcccca cagcaccggc atgatgggta agtccaggcc taaggttagg aagcaaatcc
     8161 tggagcatga ggaaattgta ggctacagtg agctaccagt ggtgtgcaaa ctggagaccc
     8221 ccaagacagt gagagaggcc acagcatctg agggaatgga gctctttctt ggcctgaggt
     8281 tcagaagaac ctgcaccaaa gaaaggcatc cctatcaatg tcactgttcc tgaaatgatg
     8341 ggagaaccac atccctgctt cagggaagca gtccctgtcg tctggggcgc tgagcccttt
     8401 ggcctgagat gaaggatgat ggtgtgatgt atcatggcag tgtgactgag actggattgg
     8461 gggatgggga caggggaaca taggcaaaaa tacacatgtg ccactggatc ctgagctgcc
     8521 attgtacctt ggaggactgg cgtttctctg ggaagttggg aggtgggaag aggaagggtc
     8581 tcattttcct gccccttgaa accatgctta ccattccttt agaagattgc tcaagctgcc
     8641 tccaattgcc tctttccaaa accaaagcat aggaaaacaa gtaaaaacag ctgaggctgc
     8701 agcataagca acttaggata gagtctagga agcaccgcca acagagaaga ctgccaagaa
     8761 acattttgag tttttcttct ctggaggtgg gtcctggttc ctcccatgga gaccacgatt
     8821 ctgtgtagtc ctgcacgctg ggcgggggat tgcctggagg tttctttaga cctgtctagc
     8881 tcacacagtc ttgatgcctg ggttttaggc tgctgtactg ttgctggggc tcacttcctg
     8941 tgggtaggct gttattttgc ccgcagatca agtcctcact gtctagatgc ctctatcatg
     9001 gggatctctt cttccctctc tggatggctc tgatccccaa gttatttcct gttgcctagg
     9061 taacacctct aattggatgc cttttaatcg ttcccttttt taaagggata aatgtggatt
     9121 ttatttccag gtcctgtcag agggccctgc cctagagaac acgtgcgccc ctgcgtgggc
     9181 aatcccttca ctgtgaccgc aaccatgggt tggatggggg gcactcactg ggctggcctg
     9241 acagtcacag tgaatcctga aagcatggtt ttcacaggaa cccaccttca ggatttagca
     9301 agactgacgt ctctcctggc cagcgctgct tcactggctt caccccagat tagggcctgt
     9361 gtttaaaaac caatcccaac tcaaatcaga aattacccaa aatagctgga gagtcactga
     9421 gatctcaggt aagctttccc ttcctgccat gaactagaag gggaaagaag agtttgacat
     9481 tcaagtttga ctctaatgct gggtgcgtga gcgcatgcgt gcatgtttgt gtgtgtgtgt
     9541 gttccacgca catttgccag ggagagagat ttcacagcat ggctccagct ggaggcggtg
     9601 aggcggtgct tttctaagac ttcctatcag aagctgtgca tactggtggg tcacgccgtg
     9661 cctgtataaa ctctggcacc tgtccttgcc ctcatcatat atgagaaaaa tgggcagaga
     9721 gagtgttcgt ttacaccccc agaccactat cctttcaatg aagcctgggt atctggcctt
     9781 cctccaggtc agggaccccc tatgctgcag aaggcaagtc tgggagaatc tgtccctcag
     9841 cccgagagca aaactgtaat cctaacatta cttccatcca ccagtttcac cagctacctc
     9901 cctcctgcct tcctctgcct ccaataggct gtgcatggag aagacaaatc ctcttgataa
     9961 acaatattta gaaagggatt ctatctttcc tgaccccaaa cacatcatgg cctctggagc
    10021 caaataccct gacatttgca agatggcttc ttttgggttc ctggtgctgc aggccctggt
    10081 tcccaaggac gcagctggca gaggtgcctc cttcagagga ggaggaggag aagctggagg
    10141 gctgcgcccg gcaaccccat gatctcttaa agggggaaaa gttgaactga tcaacagtag
    10201 ttaagaaaaa aaaaatccac accaacaaat aaatatcttg tctgagaaga ctca
//
