LOCUS       NM_001308142            8804 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens myocardin related transcription factor B (MRTFB),
            transcript variant 1, mRNA.
ACCESSION   NM_001308142 XM_005255451
VERSION     NM_001308142.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Fang,G., Tian,Y., Huang,S., Zhang,X., Liu,Y., Li,Y., Du,J. and
            Gao,S.
  TITLE     KLF15 maintains contractile phenotype of vascular smooth muscle
            cells and prevents thoracic aortic dissection by interacting with
            MRTFB
  JOURNAL   J Biol Chem 300 (5), 107260 (2024)
   PUBMED   38582447
  REMARK    GeneRIF: KLF15 maintains contractile phenotype of vascular smooth
            muscle cells and prevents thoracic aortic dissection by interacting
            with MRTFB.
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Andrews,J.C., Mok,J.W., Kanca,O., Jangam,S., Tifft,C.,
            Macnamara,E.F., Russell,B.E., Wang,L.K., Nelson,S.F., Bellen,H.J.,
            Yamamoto,S., Malicdan,M.C.V. and Wangler,M.F.
  CONSRTM   Undiagnosed Diseases Network
  TITLE     De novo variants in MRTFB have gain-of-function activity in
            Drosophila and are associated with a novel neurodevelopmental
            phenotype with dysmorphic features
  JOURNAL   Genet Med 25 (6), 100833 (2023)
   PUBMED   37013900
  REMARK    GeneRIF: De novo variants in MRTFB have gain-of-function activity
            in Drosophila and are associated with a novel neurodevelopmental
            phenotype with dysmorphic features.
REFERENCE   3  (bases 1 to 8804)
  AUTHORS   Hayashi,K., Horoiwa,S., Mori,K., Miyata,H., Labios,R.J., Morita,T.,
            Kobayashi,Y., Yamashiro,C., Higashijima,F., Yoshimoto,T., Kimura,K.
            and Nakagawa,Y.
  TITLE     Role of CRP2-MRTF interaction in functions of myofibroblasts
  JOURNAL   Cell Struct Funct 48 (1), 83-98 (2023)
   PUBMED   37164693
  REMARK    GeneRIF: Role of CRP2-MRTF interaction in functions of
            myofibroblasts.
REFERENCE   4  (bases 1 to 8804)
  AUTHORS   Sobierajska,K., Wawro,M.E. and Niewiarowska,J.
  TITLE     Oxidative Stress Enhances the TGF-beta2-RhoA-MRTF-A/B Axis in Cells
            Entering Endothelial-Mesenchymal Transition
  JOURNAL   Int J Mol Sci 23 (4), 2062 (2022)
   PUBMED   35216178
  REMARK    GeneRIF: Oxidative Stress Enhances the TGF-beta2-RhoA-MRTF-A/B Axis
            in Cells Entering Endothelial-Mesenchymal Transition.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8804)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   6  (bases 1 to 8804)
  AUTHORS   Lagna,G., Ku,M.M., Nguyen,P.H., Neuman,N.A., Davis,B.N. and Hata,A.
  TITLE     Control of phenotypic plasticity of smooth muscle cells by bone
            morphogenetic protein signaling through the myocardin-related
            transcription factors
  JOURNAL   J Biol Chem 282 (51), 37244-37255 (2007)
   PUBMED   17947237
  REMARK    GeneRIF: BMP signaling modulates VSMC phenotype via cross-talk with
            the RhoA/MRTFs pathway, and may contribute to the development of
            the pathological characteristics observed in patients with PAH and
            other obliterative vascular diseases.
REFERENCE   7  (bases 1 to 8804)
  AUTHORS   Morita,T., Mayanagi,T. and Sobue,K.
  TITLE     Dual roles of myocardin-related transcription factors in epithelial
            mesenchymal transition via slug induction and actin remodeling
  JOURNAL   J Cell Biol 179 (5), 1027-1042 (2007)
   PUBMED   18056415
  REMARK    GeneRIF: Myocardin-related transcription factors are critical
            mediators of transforming growth factor beta (TGF-beta) 1-induced
            epithelial-mesenchymal transition.[
REFERENCE   8  (bases 1 to 8804)
  AUTHORS   Selvaraj,A. and Prywes,R.
  TITLE     Megakaryoblastic leukemia-1/2, a transcriptional co-activator of
            serum response factor, is required for skeletal myogenic
            differentiation
  JOURNAL   J Biol Chem 278 (43), 41977-41987 (2003)
   PUBMED   14565952
  REMARK    GeneRIF: dominant negative MKL2 blocked differentiation-induced
            expression of SRF target genes skeletal alpha-actin and
            alpha-myosin heavy chain and blocked differentiation of the
            myoblasts to myotubes in vitro.
REFERENCE   9  (bases 1 to 8804)
  AUTHORS   Cen,B., Selvaraj,A., Burgess,R.C., Hitzler,J.K., Ma,Z., Morris,S.W.
            and Prywes,R.
  TITLE     Megakaryoblastic leukemia 1, a potent transcriptional coactivator
            for serum response factor (SRF), is required for serum induction of
            SRF target genes
  JOURNAL   Mol Cell Biol 23 (18), 6597-6608 (2003)
   PUBMED   12944485
REFERENCE   10 (bases 1 to 8804)
  AUTHORS   Wang,D.Z., Li,S., Hockemeyer,D., Sutherland,L., Wang,Z.,
            Schratt,G., Richardson,J.A., Nordheim,A. and Olson,E.N.
  TITLE     Potentiation of serum response factor activity by a family of
            myocardin-related transcription factors
  JOURNAL   Proc Natl Acad Sci U S A 99 (23), 14855-14860 (2002)
   PUBMED   12397177
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC130650.2, AC012626.10 and AC040173.5.
            
            On Aug 15, 2019 this sequence version replaced NM_001308142.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.12166.1,
                                           SRR18074969.768518.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000571589.6/ ENSP00000459626.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-47                AC130650.2         148633-148679       c
            48-112              AC130650.2         140644-140708       c
            113-329             AC130650.2         79238-79454         c
            330-395             AC130650.2         9690-9755           c
            396-451             AC130650.2         7589-7644           c
            452-527             AC130650.2         6378-6453           c
            528-689             AC130650.2         2711-2872           c
            690-868             AC130650.2         1000-1178           c
            869-1006            AC012626.10        104855-104992       c
            1007-1254           AC040173.5         186660-186907       c
            1255-1387           AC040173.5         181484-181616       c
            1388-2422           AC040173.5         179637-180671       c
            2423-2578           AC040173.5         178063-178218       c
            2579-2740           AC040173.5         175121-175282       c
            2741-2878           AC040173.5         174642-174779       c
            2879-2939           AC040173.5         168983-169043       c
            2940-8804           AC040173.5         160371-166235       c
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.12"
     gene            1..8804
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /note="myocardin related transcription factor B"
                     /db_xref="GeneID:57496"
                     /db_xref="HGNC:HGNC:29819"
                     /db_xref="MIM:609463"
     exon            1..47
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            48..112
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            113..329
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     CDS             176..3475
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /note="isoform 1 is encoded by transcript variant 1;
                     megakaryoblastic leukemia 2; MKL/myocardin-like protein 2;
                     MKL/myocardin like 2; CTA-276F8.1; MKL and myocardin
                     family member 2; MKL1/myocardin like 2"
                     /codon_start=1
                     /product="myocardin-related transcription factor B isoform
                     1"
                     /protein_id="NP_001295071.1"
                     /db_xref="CCDS:CCDS76823.1"
                     /db_xref="GeneID:57496"
                     /db_xref="HGNC:HGNC:29819"
                     /db_xref="MIM:609463"
                     /translation="MDHTGAIDTEDEVGPLAHLAPSPQSEAVAHEFQELSLQSSQNLP
                     PLNERKNVLQLRLQQRRTREQLVDQGIMPPLKSPAAFHEQIKSLERARTENFLKHKIR
                     SRPDRSELVRMHILEETFAEPSLQATQMKLKRARLADDLNEKIAQRPGPMELVEKNIL
                     PVDSSVKEAIIGVGKEDYPHTQGDFSFDEDSSDALSPDQPASQESQGSAASPSEPKVS
                     ESPSPVTTNTPAQFASVSPTVPEFLKTPPTADQPPPRPAAPVLPTNTVSSAKPGPALV
                     KQSHPKNPNDKHRSKKCKDPKPRVKKLKYHQYIPPDQKGEKNEPQMDSNYARLLQQQQ
                     LFLQLQILSQQKQHYNYQTILPAPFKPLNDKNSNSGNSALNNATPNTPRQNTSTPVRK
                     PGPLPSSLDDLKVSELKTELKLRGLPVSGTKPDLIERLKPYQEVNSSGLAAGGIVAVS
                     SSAIVTSNPEVTVALPVTTLHNTVTSSVSTLKAELPPTGTSNATRVENVHSPLPISPS
                     PSEQSSLSTDDTNMADTFTEIMTMMSPSQFLSSSPLRMTNNEDSLSPTSSTLSNLELD
                     AAEKDRKLQEKEKQIEELKRKLEQEQKLVEVLKMQLEVEKRGQQQRPLEAQPSAPGHS
                     VKSDQKHGSLGSSIKDEASLPDCSSSRQPIPVASHAVGQPVSTGGQTLVAKKAVVIKQ
                     EVPVGQAEQQSVVSQFYVSSQGQPPPAVVAQPQALLTTQTAQLLLPVSIQGSSVTSVQ
                     LPVGSLKLQTSPQAGMQTQPQIATAAQIPTAALASGLAPTVPQTQDTFPQHVLSQPQQ
                     VRKVFTNSASSNTVLPYQRHPAPAVQQPFINKASNSVLQSRNAPLPSLQNGPNTPNKP
                     SSPPPPQQFVVQHSLFGSPVAKTKDPPRYEEAIKQTRSTQAPLPEISNAHSQQMDDLF
                     DILIKSGEISLPIKEEPSPISKMRPVTASITTMPVNTVVSRPPPQVQMAPPVSLEPMG
                     SLSASLENQLEAFLDGTLPSANEIPPLQSSSEDREPFSLIEDLQNDLLSHSGMLDHSH
                     SPMETSETQFAAGTPCLSLDLSDSNLDNMEWLDITMPNSSSGLTPLSTTAPSMFSADF
                     LDPQDLPLPWD"
     exon            330..395
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            396..451
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            452..527
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            528..689
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            690..868
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1006
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1254
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1387
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..2422
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..2578
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            2579..2740
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..2878
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            2879..2939
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     exon            2940..8804
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8784..8789
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /note="hexamer: AATAAA"
     polyA_site      8804
                     /gene="MRTFB"
                     /gene_synonym="MKL2; MRTF-B; NPD001"
                     /note="major polyA site"
ORIGIN      
        1 gagatcgcgc ggcggcggcg ggagcggcgg cggcggcggc cggggaggtt cacaataaag
       61 gtgctaagaa agagaatgta agacaggaaa ataattaaat attcttaccg agtgtcttca
      121 ataggccgtg tttaagaggc gtcttacact ccctgttgcc agtggctgga acacaatgga
      181 tcacacaggg gcgatagaca ccgaggatga agtgggacct ttagcccatc ttgctccaag
      241 tcctcagagt gaagctgtgg ctcatgaatt ccaggaactc tccttgcagt ccagtcaaaa
      301 cttaccccct ctgaacgaaa ggaaaaatgt gctccagctg aggctgcaac aaaggaggac
      361 gagagaacaa ctagtggacc agggcatcat gccacctttg aagagcccag cggcattcca
      421 tgaacagata aaaagcttgg aacgagccag aactgaaaac tttttgaaac acaagattcg
      481 gagtcgacca gatcgttctg aacttgtcag gatgcacatt ttagaagaaa catttgcaga
      541 gccatccctg caggctactc agatgaagtt gaaaagagct cgactagcag atgatctgaa
      601 tgaaaagatt gctcaaagac ctggtcctat ggagctggta gagaaaaaca tccttcctgt
      661 ggactccagt gttaaagaag caattatagg cgttgggaag gaggactatc cccacactca
      721 gggcgatttc tcatttgatg aagacagcag tgacgctttg tctccggacc agcctgcgag
      781 tcaggagtca caggggtcag ccgcgtcccc aagtgagcca aaagttagtg aatcgccatc
      841 tcctgtgact acaaacactc cagcgcagtt tgcttcagtg tccccaacag ttcctgaatt
      901 cttgaaaact cctccaactg cagatcagcc tcccccacgg cctgcagctc ctgtcctccc
      961 cacaaacact gtgtcctcag caaagcctgg cccagcactg gtgaagcaaa gccatcccaa
     1021 gaatccaaat gacaaacacc gtagcaaaaa gtgcaaagat cccaaaccac gggtaaagaa
     1081 gttaaagtac caccaataca ttccaccaga tcagaagggt gagaagaatg agccgcagat
     1141 ggactctaac tacgcccgcc tgctccagca gcagcagctg ttcctgcaac tgcagatcct
     1201 gagtcagcag aagcagcact acaactacca gaccatcctg cctgcaccat tcaagccact
     1261 caatgacaaa aatagtaaca gtgggaattc agctttgaac aatgccacac ctaacacacc
     1321 aagacagaat acatctactc ctgtgagaaa gccaggacct ctgccttcta gcctggatga
     1381 cttaaaggta tcagaactga agacagaact gaagttaagg ggtctgccag tgtcaggcac
     1441 caaaccggac ctcattgagc gcctaaaacc ctaccaggaa gtgaacagca gcggccttgc
     1501 tgctgggggc atcgtggcag tgtcatcatc agccattgtc accagtaacc cagaagtcac
     1561 tgtggccttg ccggttacaa cactacacaa cactgtgact agctcagtct ctactctcaa
     1621 ggcagaattg ccacctacag gaaccagcaa cgcaacccgt gtggaaaatg ttcattcccc
     1681 tctgcccatt tcaccatctc cctccgaaca gtccagtctc agtactgatg acacaaacat
     1741 ggcagacact ttcaccgaga ttatgaccat gatgtcgcct tcacagttct tgagttcatc
     1801 tcctttgaga atgacaaata atgaagacag tctgagtccc accagcagca ctctgtcaaa
     1861 cctggaactg gatgcagccg aaaaggatcg caagcttcag gagaaagaga agcaaatcga
     1921 agagctgaag aggaaactgg aacaagagca gaagctcgtg gaagtgctga aaatgcaact
     1981 tgaggttgaa aaacgagggc agcagcagcg gcccctggaa gcccagccca gtgccccagg
     2041 tcattctgtc aagtcagatc agaagcacgg cagccttggc tcctccatca aagatgaggc
     2101 ctcactccct gactgctcca gctccaggca gcccatccca gtagccagcc acgctgtagg
     2161 ccagcccgtc tctacaggtg gccagaccct tgttgccaaa aaggctgtag ttatcaagca
     2221 agaggtccct gtgggccagg cagagcagca gagtgtcgtc tcgcagtttt atgtgagttc
     2281 ccagggacag ccaccgcctg ctgttgttgc tcagccccag gctttactga ccacgcagac
     2341 tgctcagctg ctgctcccag tgtccatcca gggctcgagt gtcacctcag tgcaactccc
     2401 tgtaggcagc ctcaaactcc agacttcacc acaagcagga atgcagactc agcctcagat
     2461 agcaactgct gcacaaatac caactgctgc cttggcctca ggcttggccc caactgtacc
     2521 tcagacacaa gacacgttcc cgcagcatgt gctcagtcag cctcaacaag tcagaaaggt
     2581 tttcacaaac tcagcatcat caaatacagt tcttccatat cagagacatc ctgccccagc
     2641 tgtccagcag ccctttatca ataaggcctc caacagtgtt cttcaatcca gaaatgctcc
     2701 gcttccatcc ctgcaaaatg gacctaacac acccaacaag cctagttcac ccccgccacc
     2761 ccagcaattt gtcgtccagc actctctatt tgggagtcca gtcgccaaga caaaagatcc
     2821 cccccgctat gaggaggcca tcaagcagac acgcagcaca caggcccctc tgccagagat
     2881 ttccaacgct cacagtcagc agatggatga cctctttgat atcctcatta agagtggaga
     2941 gatctccctc cccataaaag aagaaccttc tcctatttcc aaaatgagac cagtgacagc
     3001 cagcatcacc acaatgccag tgaatacagt ggtgtcccgg ccaccacccc aagtccaaat
     3061 ggcaccacct gtatctttag aacctatggg cagtttatct gccagcttag agaaccaact
     3121 agaagctttc ttggatggaa ctttaccctc agccaatgaa attcctccac tacaaagcag
     3181 cagtgaagac agagagccct tctctctgat cgaggacctc cagaatgatc tgctgagtca
     3241 ctcaggtatg ctggaccatt cacactcacc catggagact tccgagaccc agtttgctgc
     3301 aggtactccc tgtctgtctc tcgacctgtc agactcaaac ttggacaaca tggagtggtt
     3361 ggacattacc atgcccaact cctcttcagg actcactcct ctcagcacca ccgcgccgag
     3421 catgttctct gctgactttc tagacccaca ggacctaccg ctgccatggg actaacgtca
     3481 cagatttctt ttctgagagt tgatgaggtt taagaacatg aagattctaa aaggtcagtt
     3541 tttagagata gatctatagt tgcattgttg caatcaaaat atgttgtcac agaaagaata
     3601 ggtggaaggt catagcctgg aacccaagtt tgaaaacatt tcattgtgtt cagtagtgaa
     3661 tttctacagt ttaacatagc acagggcctt ctgaaaatcg cacttgtcaa agacgactca
     3721 tctatttctc cagacttcag taaagaatga aaagtacctt tagataaaaa caaagaagag
     3781 taatatatgc agcacagtga cgttaggatt ctggtaatta actacattta aatctctggt
     3841 cactttaaga ccctaaataa aaggcagaca gctccactca aaaactaagg ctgatgtgag
     3901 ggaggtgaga ggtcactgca cttggtactt cctagagacg gccggagcca ggcccaagac
     3961 acagcagagg tcaaaggcag tggagagcct tggccagttc agtgacagct tctggctgaa
     4021 gacttttggt tctattcaga aacctgtgtc gtttttttgg tgttgtagtt tattttgtga
     4081 ttttttggaa tcgtacttta tatttccaaa tttaaattta aatgcaagat ctttcaacat
     4141 aaacagaaga tacctacaaa atactgtcag aagtccaggt atactgataa cactgaaaat
     4201 tctattagca accttctggg ttggttagat tgattttaat gtatatatta gacatttgta
     4261 tgtatgctct gacattgtga tttgtacagc ctacgctggg gtaaggaaat gggtatccaa
     4321 ggtcctactt ttttaatagc tcgaatattt ctagagtact tgagccacat gtatttctgt
     4381 atttaaagaa ttgctgacta actttcaggt aaccagaccc atctcaaaga accaagaaaa
     4441 ggctttagca tgaaatatct ttctgagctg gcgagttaga agaatggaag gtaaggggaa
     4501 ggtctgtcat ctacccagga cattcccatg atgagtacag gtcagattgt gccacaaggt
     4561 gggcctccac gtccctgccc tggccctctt tcttctgtca ctaaccctgg ttatcatttt
     4621 acaggcttgt aactggatat tctaccagag ctctcactat attgtcaagc ctaagattga
     4681 aaaactggag gctttattag tgtttttata tagaaaacag ttattacata tgtgttaagt
     4741 catttcttaa gaattttcta aaatgccaac tatcacagga ttatttcaag ctagtcattg
     4801 aggtatatga caaaatgtaa ataacaaaaa actgaaatct accaaaagag catggagatt
     4861 tttcttaaat aataatattg tgctctccac ctcacccttg tgtaaaccct caggtcaggt
     4921 tggttcccct gggtcacaaa atggtaaatg ttccatactg acatgccgga ggcagcctga
     4981 cccgttattt ggaaagaatt tgtgaattat ttctgggttt gtgttttgcc taagagctga
     5041 tcttatttct gatttgtgtg tgtgtggttt ataaatttta ctacgtgtaa caaaagtctg
     5101 cttttccagc atgagcacac tggagccctc tgctcactgg cacttcctga ctcaaaggca
     5161 ctaacagtgc tcatgtgcct tccagacggt tcaaggcaga ggccactgtg ctcagtgtag
     5221 tctgtgatga atagtttaag tgttcagaaa ttggtaaacc aacacacggg atacaataac
     5281 tctctgttaa ccagaacacc gtcatttgac caagccctac aagagtttac cacactagat
     5341 gttctgtgaa tcctcgagtc agttccattg agaggggcct gtctccaggg ccaggctatt
     5401 tacttgggaa agtatttttc ttacagttct tggccacagt ttttttgctg agggtttctg
     5461 tctgggccta tcaggtccat acttagaccc tgagcatctt cttcattcag atttgaatgg
     5521 cttattaaat tagcaccaaa tatcagtggg actgtagaag gtaaccgaac actagtacca
     5581 ttgactctcg ttgaataact ttatatttcc ataatcctga attgtgtaga tagttttcta
     5641 gctctcgggt ccctttattg ctttttaaaa tagggtttga aacatgccac aggaaggttg
     5701 ctctccaaaa atacacagtg cagtgcaaga aaatgctatc tcattggcct actctcctat
     5761 gaattgctaa agtgcccact tcacatatgt gtttaaacct ttataaacca gtatttcact
     5821 tttaaaaaga caaggcatct ctacccatta actctgcaag ccactccact tgcaccattc
     5881 cgcttgaccc tcctctctcc tggcttgggt caccagccag gcacctgtga cacgagtgct
     5941 gctctccagg atctccacta catgttccag gttggagtga ggacgcgcta tgtgctcaca
     6001 ctcatgtgac atgaccaaag atgatactct gtaaacaagg cccttctgac cggactcagt
     6061 gcgtgtgatg gtgagtgcaa tgaggaaggg tggatattga ctaaagactg gttgttgttg
     6121 ttgtttaggt tagtgtcaca gccattacag cacaagtcaa agtcgccagt tgaattttca
     6181 ttatgcacgt gtgtggttta agcagtggta ctgttgtata tcatattgta aagtatcata
     6241 ctgccaagaa aataactcct agaaaggcat tatctcacat ccccatctta ctttcctaca
     6301 tgttctcaaa acacagtagt accagcctta cctttctgct gtatgtagat agtcagatca
     6361 tcctactggg ggtgggatga atttaaaagt tataccaaaa tttctgtaaa gtctttgaaa
     6421 gtctaggagt aggtggtcat cttgtacatt tcagcaaagc atccactagg aaacctcata
     6481 ggacagtgtt agtggttcac gttctagtgt ttttcctgaa atgtgcaatc tactgtatag
     6541 tattgccaca taattgtaca tagatgtatt ctgaatttgt ggaatttctt gcttagatac
     6601 tattgtgttt gtttcatatg aatatttttg taattctaaa agagatctta tttaaatttc
     6661 ctttttaaaa gccgcatggt tctgtgatcc atgtaactga cactttttgg ctttcagtgc
     6721 tgtttagaaa cagtggcaaa ggcagggtgg tgctgcctgc aagctgctgc ctatggaagg
     6781 caaagtcata gtaatgagat agcacctctg aactgtgcag tcagcatacc ctgagtgatg
     6841 gctcaggggc gcactaccta ttttgtcacc agagctgact caggcttcct ggaccctcaa
     6901 ccccagatca ttccaggcag catagctttc ttcacagtcc tttcagaatt cccaggctga
     6961 aatcagccat agcagttgac aaaacagcta tccccacaag tgatgagata gtccctttac
     7021 tgtcctcaaa tggacttggc cttagagacg tggtaaagca ctttggcagg gtttaaaata
     7081 tttgtgagaa gcccacattt cagtatacat cctcactggc ttccatgcac ccacctgacg
     7141 gtagcctcac agaagtcctg gctgtcactc aggtggggag ctcatggtgc cgctggggac
     7201 tttttagaga aatgtaaaga gaatagctat tcagtgtcta ctagcagagc aacatgtgtc
     7261 aatttaacca aattcacaaa taaccctcca tttttcaata tctgctactg taaacatgaa
     7321 tattgaatac tgacaagaga atacccatac aaatcgtccc accgccctag aggccacaga
     7381 attagcccaa aattatcaaa gaacattaac tggaaggtca ggtttttcaa ggaacatagc
     7441 ttacaaatgc atcagtgtgt atctggagag catcctaact gcatttcaac tcatctttta
     7501 agtgatttca gtcaaaactg gaaaacaact aagatgtagt aatttttttt tcctggttca
     7561 aaccttcaat aacttgctca ttcagcagtc tctctgagct acatttttat ttgtaaagtg
     7621 actctgtctg catggcaatg agcaggtgcg tgttttttcc acattcgccc tttcttgcag
     7681 tatccaggga aacacatcat tacaaagggt ttctacctga aatctttcat ggaaggccta
     7741 caattcgaaa agctgcacat gtttacagaa gagctcttac cctccatgca aacactttgc
     7801 tctgtggtgt cacagctttg tgacaataag atggcaatct cggatctaca aggtgctgtc
     7861 gggaatcaaa taaaatatgt tatcagagat atcatcacat ctatagtgtt taacagagct
     7921 ttatgccaac tactaagaca aagctttaac aaagtttata gaatactgaa actcgtaaca
     7981 attacctctc tacgtgatgc tgtaaggaat cttgctaatt tggtaggaag aggaagcatt
     8041 taggaaaggg tttagtatct accaaaagta cttgacctca agtaaccaat agtaatgcaa
     8101 acttgcttta aaggaaccaa aggcattgcc aagtatttgc caaaaggagg cactttttat
     8161 ttaaaatttg agactaatga gatctcaaaa atcagcccca aaaaggtatt atccatttaa
     8221 ggttataatt ttcactaaga tgtagatatt tctcttattg ttttcatgta aaatagtata
     8281 gagttgtttt gttggtttaa gagtaacatt cagtagtaat acaaagtttt ttttctatgt
     8341 agaaattagt tttcttttct tgcttgcaat agaaatgcaa tgtgataggt gtttctcttc
     8401 ttattttcat tgtcacatta tgtcttagca tctcacttta tgaaaaaaag gagaaagata
     8461 ccaatctaca gagccctgct tgttgaagca ctagtttaat caacaaaaaa ttatggcaat
     8521 cgggggtcca ttcatctatt gcctttatgt tgttttttaa aagaaaaacc atgatgcctt
     8581 tgtatttgct gtttgcaccc ctgaaatcaa ttccatatca tgtttgaatg ccatacattt
     8641 tgcacatgta ctgtacataa gtaatgcata ctgtattttt atatgtgtgc acatttatca
     8701 tcagatcttt tgtacatagt ggcagtattg tagctgatcg ggaaatgttt gatatctcag
     8761 caattttgca tttttgtgtc tcaaataaaa gacattttga tgta
//
