LOCUS       NM_020662               3939 bp    mRNA    linear   PRI 05-AUG-2024
DEFINITION  Homo sapiens magnesium transporter MRS2 (MRS2), transcript variant
            2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_020662
VERSION     NM_020662.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Uthayabalan,S., Lake,T. and Stathopulos,P.B.
  TITLE     MRS2 missense variation at Asp216 abrogates inhibitory Mg2+
            binding, potentiating cell migration and apoptosis resistance
  JOURNAL   Protein Sci 33 (8), e5108 (2024)
   PUBMED   38989547
  REMARK    GeneRIF: MRS2 missense variation at Asp216 abrogates inhibitory
            Mg[2+] binding, potentiating cell migration and apoptosis
            resistance.
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Ponnusamy,T., Velusamy,P. and Shanmughapriya,S.
  TITLE     Mrs2-mediated mitochondrial magnesium uptake is essential for the
            regulation of MCU-mediated mitochondrial Ca2+ uptake and viability
  JOURNAL   Mitochondrion 76, 101877 (2024)
   PUBMED   38599304
  REMARK    GeneRIF: Mrs2-mediated mitochondrial magnesium uptake is essential
            for the regulation of MCU-mediated mitochondrial Ca[2+] uptake and
            viability.
REFERENCE   3  (bases 1 to 3939)
  AUTHORS   Lai,L.T.F., Balaraman,J., Zhou,F. and Matthies,D.
  TITLE     Cryo-EM structures of human magnesium channel MRS2 reveal gating
            and regulatory mechanisms
  JOURNAL   Nat Commun 14 (1), 7207 (2023)
   PUBMED   37938562
  REMARK    GeneRIF: Cryo-EM structures of human magnesium channel MRS2 reveal
            gating and regulatory mechanisms.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3939)
  AUTHORS   Li,M., Li,Y., Lu,Y., Li,J., Lu,X., Ren,Y., Wen,T., Wang,Y.,
            Chang,S., Zhang,X., Yang,X. and Shen,Y.
  TITLE     Molecular basis of Mg2+ permeation through the human mitochondrial
            Mrs2 channel
  JOURNAL   Nat Commun 14 (1), 4713 (2023)
   PUBMED   37543649
  REMARK    GeneRIF: Molecular basis of Mg[2+] permeation through the human
            mitochondrial Mrs2 channel.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3939)
  AUTHORS   Uthayabalan,S., Vishnu,N., Madesh,M. and Stathopulos,P.B.
  TITLE     The human MRS2 magnesium-binding domain is a regulatory feedback
            switch for channel activity
  JOURNAL   Life Sci Alliance 6 (4), e202201742 (2023)
   PUBMED   36754568
  REMARK    GeneRIF: The human MRS2 magnesium-binding domain is a regulatory
            feedback switch for channel activity.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3939)
  AUTHORS   Wolf,F.I. and Trapani,V.
  TITLE     Multidrug resistance phenotypes and MRS2 mitochondrial magnesium
            channel: two players from one stemness?
  JOURNAL   Cancer Biol Ther 8 (7), 615-617 (2009)
   PUBMED   19270501
  REMARK    GeneRIF: Multidrug resistance phenotypes is related to MRS2
            mitochondrial magnesium channel.
REFERENCE   7  (bases 1 to 3939)
  AUTHORS   Chen,Y., Wei,X., Yan,P., Han,Y., Sun,S., Wu,K. and Fan,D.
  TITLE     Human mitochondrial Mrs2 protein promotes multidrug resistance in
            gastric cancer cells by regulating p27, cyclin D1 expression and
            cytochrome C release
  JOURNAL   Cancer Biol Ther 8 (7), 607-614 (2009)
   PUBMED   19242098
  REMARK    GeneRIF: By enhanced hsaMrs2p expression, p27 was downregulated
            whereas cyclinD1 was upregulatedin gastric cancer.
REFERENCE   8  (bases 1 to 3939)
  AUTHORS   Piskacek,M., Zotova,L., Zsurka,G. and Schweyen,R.J.
  TITLE     Conditional knockdown of hMRS2 results in loss of mitochondrial
            Mg(2+) uptake and cell death
  JOURNAL   J Cell Mol Med 13 (4), 693-700 (2009)
   PUBMED   18384665
  REMARK    GeneRIF: hMrs2 is the major transport protein for Mg (+) uptake
            into mitochondria and that expression of hMrs2 is essential for the
            maintenance of respiratory complex I and cell viability.
REFERENCE   9  (bases 1 to 3939)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   10 (bases 1 to 3939)
  AUTHORS   Zsurka,G., Gregan,J. and Schweyen,R.J.
  TITLE     The human mitochondrial Mrs2 protein functionally substitutes for
            its yeast homologue, a candidate magnesium transporter
  JOURNAL   Genomics 72 (2), 158-168 (2001)
   PUBMED   11401429
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM829320.1, AK291058.1, AF293076.1, AL359713.25 and AA836333.1.
            
            On Jun 3, 2019 this sequence version replaced NM_020662.3.
            
            Transcript Variant: This variant (2) lacks an exon and uses an
            alternate splice site, which results in an alternate in-frame
            segment in the 5' coding region, compared to variant 1. The encoded
            isoform (b) is shorter than isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.220032.1,
                                           SRR1660805.214239.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378386.8/ ENSP00000367637.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BM829320.1         29-51
            24-575              AK291058.1         1-552
            576-761             AF293076.1         516-701
            762-983             AK291058.1         739-960
            984-1472            AF293076.1         924-1412
            1473-3565           AL359713.25        120243-122335
            3566-3939           AA836333.1         17-390              c
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p22.3"
     gene            1..3939
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /note="magnesium transporter MRS2"
                     /db_xref="GeneID:57380"
                     /db_xref="HGNC:HGNC:13785"
                     /db_xref="MIM:619307"
     exon            1..301
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..1443
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; magnesium transporter MRS2 homolog,
                     mitochondrial; MRS2-like protein; MRS2-like, magnesium
                     homeostasis factor; putative magnesium transporter; MRS2,
                     magnesium transporter"
                     /codon_start=1
                     /product="magnesium transporter MRS2 homolog,
                     mitochondrial isoform b precursor"
                     /protein_id="NP_065713.1"
                     /db_xref="CCDS:CCDS4552.1"
                     /db_xref="GeneID:57380"
                     /db_xref="HGNC:HGNC:13785"
                     /db_xref="MIM:619307"
                     /translation="MECLRSLPCLLPRAMRLPRRTLCALALDVTSVGPPVAACGRRAN
                     LIGRSRAAQLCGPDRLRVAGEVHRFRTSDVSQATLASVAPVFTVTKFDKQGNVTSFER
                     KKTELYQELGLQARDLRFQHVMSITVRNNRIIMRMEYLKAVITPECLLILDYRNLNLE
                     QWLFRELPSQLSGEGQLVTYPLPFEFRAIEALLQYWINTLQGKLSILQPLILETLDAL
                     VDPKHSSVDRSKLHILLQNGKSLSELETDIKIFKESILEILDEEELLEELCVSKWSDP
                     QVFEKSSAGIDHAEEMELLLENYYRLADDLSNAARELRVLIDDSQSIIFINLDSHRNV
                     MMRLNLQLTMGTFSLSLFGLMGVAFGMNLESSLEEDHRIFWLITGIMFMGSGLIWRRL
                     LSFLGRQLEAPLPPMMASLPKKTLLADRSMELKNSLRLDGLGSGRSILTNR"
     exon            302..375
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            376..412
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            413..525
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            526..699
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            700..830
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            831..947
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1100
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1218
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1332
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..3939
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3313..3318
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /note="hexamer: AATAAA"
     polyA_site      3335
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /note="major polyA site"
     regulatory      3923..3928
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
                     /note="hexamer: GATAAA"
     polyA_site      3939
                     /gene="MRS2"
                     /gene_synonym="HPT; MRS2L"
ORIGIN      
        1 acagagtctg caggtcgggc ggtagcgaca ggtcagagct gcggcctgag cagccagcgt
       61 ccggcatgaa ggtctggggt ctggctgctg cctgcttctt gctccagcac catggaatgc
      121 ctgcgcagtt taccctgcct cctgccccgc gcgatgagac ttccccggcg gacgctgtgt
      181 gccctggcct tggacgtgac ctctgtgggt cctcccgttg ctgcctgcgg ccgccgagcc
      241 aacctgattg gaaggagccg agcggcgcag ctttgcgggc ccgaccggct ccgcgtggca
      301 ggtgaagtgc accggtttag aacctctgac gtctctcaag ccactttagc cagtgtagcc
      361 ccagtattta ctgtgacaaa atttgacaaa cagggaaacg ttacttcttt tgaaaggaag
      421 aaaactgaat tataccaaga gttaggtctt caagccagag atttgagatt tcagcatgta
      481 atgagtatca cagtcagaaa caataggatt atcatgagaa tggagtattt gaaagctgtg
      541 ataactccag agtgtcttct gatattagat tatcgtaatt taaacttaga gcaatggctg
      601 ttccgggaac tcccttcaca gttgtctgga gagggtcaac tcgttacata ccctttacct
      661 tttgagttta gagctataga agcactcctg caatattgga tcaacaccct tcaggggaaa
      721 cttagcattt tgcagccact gatccttgag accttggatg ctttggtgga ccccaaacat
      781 tcttctgtag acagaagcaa actgcacatt ttactacaga atggcaaaag tctatcagag
      841 ttagaaacag atattaaaat tttcaaagag tcaattttgg agatcttgga tgaggaagag
      901 ttgctagaag agctctgtgt atcaaaatgg agtgacccac aagtctttga aaagagcagt
      961 gctgggattg accatgcaga agagatggag ttgctgttgg aaaactacta ccgattggct
     1021 gacgatctct ccaatgcagc tcgtgagctt agggtgctga ttgatgattc acaaagtatt
     1081 attttcatta atctggacag ccaccgaaac gtgatgatga ggttgaatct acagctgacc
     1141 atgggaacct tctctctttc gctctttgga ctaatgggag ttgcttttgg aatgaatttg
     1201 gaatcttccc ttgaagagga ccatagaatt ttttggctga ttacaggaat tatgttcatg
     1261 ggaagtggcc tcatctggag gcgcctgctt tcattccttg gacgacagct agaagctcca
     1321 ttgcctccta tgatggcttc tttacctaaa aagactcttc tggcagatag aagcatggaa
     1381 ttgaaaaata gcctcagact ggatggactt ggatcaggaa ggagcatcct aacaaaccgt
     1441 taggaacagc cccgtggata ctgaagtttt ttttatggta gttacaggaa acttctgata
     1501 ctctttttat tattttcttg tatagagtca gacacttgaa aaaaactaat gtttgaagac
     1561 aaaaatattt tggcagtcac aataccagaa ctggattgca tttccagaat tctgagttaa
     1621 agaaacaaag tatttgcttt gtaaaaggcc aaaattctat ttcctacaaa ctttaaatgc
     1681 tgtttttata gatgtgatat gaggcaacac aagcacagac agttgcatag attttaattt
     1741 atacatatca agaaaagtgc aatttcatgc tgaatgaagc gtaggaactt gacaagccca
     1801 taggtagcta tagttctttg tcagtatagg gaattatgtt catgtgaatt tcctgattct
     1861 caggtgacta aaaagctagc attctatgta ttaaccttac aacagactct gtaagtttga
     1921 gctttaaaaa ccaaactttg acataacctt atttcttgta tttgccccct tttttttata
     1981 aaaggtgaat aaaaagaaat aatttaatat caccattgta tggattccta atcaagattt
     2041 cacgttctca gcccctgaga ctagttttct ttgctctctg taattagagc ctttggaagc
     2101 aaagttgaaa ggaagtattt ccattctgtt actgttttgt agcactttgt ccatttattg
     2161 atttttaaag tagatattta gataccaccc ctgccctgcc ccaaaaagaa aaatgtttat
     2221 tgtcctgcta atctatatgc ctacacctca gaagctgaca gatgagcttc ctaaaacatg
     2281 aggatgaata aatgttagaa ctgtgatatc ttctcatttc cacagagatt tgggtttaaa
     2341 acaaatggct aatatttgta agcctgaaag ctgccatcct ctttaacatc ctacctacct
     2401 tttaaaatat tttctggagg ttaagtacag ttaggcacta gaacatttgt taagcctccc
     2461 aaagtagtgt gcatggaaga ttctagagtg tccagctctt gcactacaaa tgtaataata
     2521 acagaataaa tacacttacc ctgatgatat tgagggtaca tgattagcct aatgtgatgt
     2581 catatgagga agtttaggag tccatttttc aggtggtttg gtgataggaa taaaaatgtt
     2641 actcccgtcg ctgatttgga agaaaaccga tgaagtacaa gtaaggtttc cccactcctg
     2701 tgtgtggtca tgggaagcca ttagagggaa gctgcagaac agaatggaac aggacaggct
     2761 gggctagact cgagcacgca gtggcccctg tgccaccaca ctgtgggtca ggaacacgcg
     2821 tcatggtgtt cttgtcggaa tgtgaacata cagcaccctc tagcattacg aatctcttag
     2881 gatttttaag attgtatttg ataatactta tatttcacag ggcacacttt tggcacacct
     2941 cagagcacac tgctgctatt ttgggtcgta tcactgtaaa atacataata agtactactt
     3001 aactgtgaca tgaagaattg gaatcccaga gggcaacatt tgatttgact aagatcaggc
     3061 ataagataga atttttgtca tttttccttg cagttttatt gacttagttt atgagcttgg
     3121 ataaaataat tttttgatga atcatgtcaa taaaaggaaa aataatgtaa ctacctcata
     3181 agtctgataa aaggaagttg ctagtgtttt atagaatttc tgaaggtggt taaatcaagt
     3241 atgatttcaa aatatcaact agttccactt ttgtgattgc aggatgcttc ttatactaaa
     3301 gttctcataa ataataaatc agcttatgcc aaaaatatat gaagttcctt ggttttaaat
     3361 tttgaactaa aatggactga gcacattaat tactgtttga aagtaggtac caaacactgg
     3421 gttatgtgag tgagtaagca atcatatcgc ctgtcaaaca catcaagtgt cgaacacgtc
     3481 aaggtgtgat actgtctttg gaaggctcct aaacaaatgt gagcacaatt attaacacct
     3541 tattcttcat gaacacagac tgttaagcag cactaaccaa ttttatatta aaaagctatt
     3601 tattttggtg agttattcca agagttgtat acagctttat tttttggaat tgcaatatta
     3661 aagttgcacg ttggatttaa ctctcatgac tttagtaata cctacagagg tgagctaatg
     3721 gatggtacat ggagtaggga ctgcagagac cctagttttg tccccactcc atcttcaaat
     3781 aattttggtc tcttaggtct gtgatacaaa ggcataaaac aattcctatc ttgaagcctt
     3841 gagaaaagct gttacatata cgcagatagt agcaagacag aaaatgcaaa tatgtaaatg
     3901 tcttctgata tcttgaaata aagataaatt tcagttaaa
//
