LOCUS       NM_138777               9594 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosome recycling factor (MRRF),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_138777
VERSION     NM_138777.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 9594)
  AUTHORS   Kummer,E., Schubert,K.N., Schoenhut,T., Scaiola,A. and Ban,N.
  TITLE     Structural basis of translation termination, rescue, and recycling
            in mammalian mitochondria
  JOURNAL   Mol Cell 81 (12), 2566-2582 (2021)
   PUBMED   33878294
REFERENCE   3  (bases 1 to 9594)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 9594)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 9594)
  AUTHORS   Tsuboi,M., Morita,H., Nozaki,Y., Akama,K., Ueda,T., Ito,K.,
            Nierhaus,K.H. and Takeuchi,N.
  TITLE     EF-G2mt is an exclusive recycling factor in mammalian mitochondrial
            protein synthesis
  JOURNAL   Mol Cell 35 (4), 502-510 (2009)
   PUBMED   19716793
  REMARK    GeneRIF: EF-G2mt is an exclusive recycling factor in mammalian
            mitochondrial protein synthesis.
REFERENCE   6  (bases 1 to 9594)
  AUTHORS   Rorbach,J., Richter,R., Wessels,H.J., Wydro,M., Pekalski,M.,
            Farhoud,M., Kuhl,I., Gaisne,M., Bonnefoy,N., Smeitink,J.A.,
            Lightowlers,R.N. and Chrzanowska-Lightowlers,Z.M.
  TITLE     The human mitochondrial ribosome recycling factor is essential for
            cell viability
  JOURNAL   Nucleic Acids Res 36 (18), 5787-5799 (2008)
   PUBMED   18782833
  REMARK    GeneRIF: Depletion of mtRRF in human cell lines is lethal,
            initially causing profound mitochondrial dysmorphism, aggregation
            of mitoribosomes and elevated mitochondrial superoxide production.
REFERENCE   7  (bases 1 to 9594)
  AUTHORS   Humphray,S.J., Oliver,K., Hunt,A.R., Plumb,R.W., Loveland,J.E.,
            Howe,K.L., Andrews,T.D., Searle,S., Hunt,S.E., Scott,C.E.,
            Jones,M.C., Ainscough,R., Almeida,J.P., Ambrose,K.D., Ashwell,R.I.,
            Babbage,A.K., Babbage,S., Bagguley,C.L., Bailey,J., Banerjee,R.,
            Barker,D.J., Barlow,K.F., Bates,K., Beasley,H., Beasley,O.,
            Bird,C.P., Bray-Allen,S., Brown,A.J., Brown,J.Y., Burford,D.,
            Burrill,W., Burton,J., Carder,C., Carter,N.P., Chapman,J.C.,
            Chen,Y., Clarke,G., Clark,S.Y., Clee,C.M., Clegg,S., Collier,R.E.,
            Corby,N., Crosier,M., Cummings,A.T., Davies,J., Dhami,P., Dunn,M.,
            Dutta,I., Dyer,L.W., Earthrowl,M.E., Faulkner,L., Fleming,C.J.,
            Frankish,A., Frankland,J.A., French,L., Fricker,D.G., Garner,P.,
            Garnett,J., Ghori,J., Gilbert,J.G., Glison,C., Grafham,D.V.,
            Gribble,S., Griffiths,C., Griffiths-Jones,S., Grocock,R., Guy,J.,
            Hall,R.E., Hammond,S., Harley,J.L., Harrison,E.S., Hart,E.A.,
            Heath,P.D., Henderson,C.D., Hopkins,B.L., Howard,P.J., Howden,P.J.,
            Huckle,E., Johnson,C., Johnson,D., Joy,A.A., Kay,M., Keenan,S.,
            Kershaw,J.K., Kimberley,A.M., King,A., Knights,A., Laird,G.K.,
            Langford,C., Lawlor,S., Leongamornlert,D.A., Leversha,M., Lloyd,C.,
            Lloyd,D.M., Lovell,J., Martin,S., Mashreghi-Mohammadi,M.,
            Matthews,L., McLaren,S., McLay,K.E., McMurray,A., Milne,S.,
            Nickerson,T., Nisbett,J., Nordsiek,G., Pearce,A.V., Peck,A.I.,
            Porter,K.M., Pandian,R., Pelan,S., Phillimore,B., Povey,S.,
            Ramsey,Y., Rand,V., Scharfe,M., Sehra,H.K., Shownkeen,R.,
            Sims,S.K., Skuce,C.D., Smith,M., Steward,C.A., Swarbreck,D.,
            Sycamore,N., Tester,J., Thorpe,A., Tracey,A., Tromans,A.,
            Thomas,D.W., Wall,M., Wallis,J.M., West,A.P., Whitehead,S.L.,
            Willey,D.L., Williams,S.A., Wilming,L., Wray,P.W., Young,L.,
            Ashurst,J.L., Coulson,A., Blocker,H., Durbin,R., Sulston,J.E.,
            Hubbard,T., Jackson,M.J., Bentley,D.R., Beck,S., Rogers,J. and
            Dunham,I.
  TITLE     DNA sequence and analysis of human chromosome 9
  JOURNAL   Nature 429 (6990), 369-374 (2004)
   PUBMED   15164053
REFERENCE   8  (bases 1 to 9594)
  AUTHORS   Hansen,L.L., Jorgensen,R. and Justesen,J.
  TITLE     Assignment of the human mitochondrial translational release factor
            1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human
            mitochondrial ribosome recycling factor (MRRF) to chromosome
            9q32-->q34.1 with radiation hybrid mapping
  JOURNAL   Cytogenet Cell Genet 88 (1-2), 91-92 (2000)
   PUBMED   10773675
REFERENCE   9  (bases 1 to 9594)
  AUTHORS   Zhang,Y. and Spremulli,L.L.
  TITLE     Identification and cloning of human mitochondrial translational
            release factor 1 and the ribosome recycling factor
  JOURNAL   Biochim Biophys Acta 1443 (1-2), 245-250 (1998)
   PUBMED   9838146
REFERENCE   10 (bases 1 to 9594)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL162424.20, AK291603.1,
            BC013049.1 and AA873020.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_138777.4.
            
            Summary: This gene encodes a ribosome recycling factor, which is a
            component of the mitochondrial translational machinery. The encoded
            protein, along with mitochondrial elongation factor 2, functions in
            ribosomal recycling at the termination of mitochondrial translation
            by mediating the disassembly of ribosomes from messenger RNA. A
            pseudogene of this gene has been identified on chromosome X.
            [provided by RefSeq, Oct 2016].
            
            Transcript Variant: Variants 1 and 5 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.331392.1,
                                           SRR3476690.50162.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            MANE Ensembl match                 :: ENST00000344641.8/
                                                  ENSP00000343867.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AL162424.20        100118-100148
            32-1066             AK291603.1         1-1035
            1067-1721           BC013049.1         1007-1661
            1722-3082           AL162424.20        158701-160061
            3083-3572           AA873020.1         14-503              c
            3573-9594           AL162424.20        160552-166573
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.2"
     gene            1..9594
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /note="mitochondrial ribosome recycling factor"
                     /db_xref="GeneID:92399"
                     /db_xref="HGNC:HGNC:7234"
                     /db_xref="MIM:604602"
     exon            1..57
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /inference="alignment:Splign:2.1.0"
     exon            58..269
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..874
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; ribosome-recycling factor, mitochondrial;
                     ribosome-releasing factor, mitochondrial"
                     /codon_start=1
                     /product="ribosome-recycling factor, mitochondrial isoform
                     1 precursor"
                     /protein_id="NP_620132.1"
                     /db_xref="CCDS:CCDS6840.1"
                     /db_xref="GeneID:92399"
                     /db_xref="HGNC:HGNC:7234"
                     /db_xref="MIM:604602"
                     /translation="MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQY
                     MAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDN
                     FNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIK
                     AIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNK
                     LKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG"
     exon            270..425
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /inference="alignment:Splign:2.1.0"
     exon            426..544
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /inference="alignment:Splign:2.1.0"
     exon            545..636
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /inference="alignment:Splign:2.1.0"
     exon            637..796
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /inference="alignment:Splign:2.1.0"
     exon            797..9594
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1691..1696
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /note="hexamer: AATAAA"
     polyA_site      1721
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /note="major polyA site"
     regulatory      3532..3537
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /note="hexamer: AATAAA"
     polyA_site      3572
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
     regulatory      7059..7064
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /note="hexamer: AATAAA"
     polyA_site      7078
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
     regulatory      9569..9574
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
                     /note="hexamer: ATTAAA"
     polyA_site      9594
                     /gene="MRRF"
                     /gene_synonym="MRFF; MTRRF; RRF"
ORIGIN      
        1 gcagctgcgg tcgccgccgt cgcacgagtc tttccttagt aacctgggcg atagctgtgg
       61 atgtttccaa ggattgtctt cagtcatggc cttgggatta aagtgcttcc gcatggtcca
      121 ccctaccttt cgcaattatc ttgcagcctc tatcagaccc gtttcagaag ttacactgaa
      181 gacagtgcat gaaagacaac atggccatag gcaatacatg gcctattcag ctgtaccagt
      241 ccgccatttt gctaccaaga aagccaaagc caaagggaaa ggacagtccc aaaccagagt
      301 gaatattaat gctgccttgg ttgaggatat aatcaacttg gaagaggtga atgaagaaat
      361 gaagtctgtg atagaagctc tcaaggataa tttcaataag actctcaata taaggacctc
      421 accaggatcc cttgacaaga ttgctgtggt aactgctgac gggaagcttg ctttaaacca
      481 gattagccag atctccatga agtcgccaca gctgattttg gtgaatatgg ccagcttccc
      541 agagtgtaca gctgcagcta tcaaggctat aagagaaagt ggaatgaatc tgaacccaga
      601 agtggaaggg acgctaattc gggtacccat tccccaagta accagagagc acagagaaat
      661 gctggtgaaa ctggccaaac agaacaccaa caaggccaaa gactctttac ggaaggttcg
      721 caccaactca atgaacaagc tgaagaaatc caaggataca gtctcagagg acaccattag
      781 gctaatagag aaacagatca gccaaatggc cgatgacaca gtggcagaac tggacaggca
      841 tctggcagtg aagaccaaag aactccttgg atgaaagtcc actggggcca gcaatactcc
      901 agagcccagt ttctgctgga tcccatgggt ggcacattgg gacttctctc cctcccccat
      961 ctacacagaa gactgtcacc atgctgacag aagcctgtcc ttgtaaggcc cagccttcca
     1021 ggggaacact cagacatgtt cattctcttc ctgcttctgc tctgggccgg tgggtggctc
     1081 tcagaaaata cttgctgctg gcaaaaggcc tgtactcagg catttgcttt gacttgatgt
     1141 tgccaaggga ctgaggccat tggcaggctt agtaccacct gctcctcatc ttaggagtct
     1201 ccttttcaaa taattaggct ctgttcccat tttaaaactc tgatattggc cttcacctgt
     1261 gactggacac tttactagag gcccattttc actaaacaat aaaatctaaa taaattggaa
     1321 ggaataacaa ccacaaagga aagaatagag ttggtctgga ttgatgatca ctgaggatct
     1381 gtatgtgagg cacccataac agtagttttg cctgtgagtc gtcttcacac atgctgtttt
     1441 ctctgcctgg ctctctcttc ccctccttac ctggccagtc ctgtttatca tcaggccttg
     1501 tcttggatat cacgtcctct gggaagtctt cttttcccct ctaacctagg accctcatta
     1561 ccggctctca tagcacagtc tactgctttg tacgaattct aagtattctt gttgcactta
     1621 attagcctgt atatcctcag aactttgtgt aatgcctgga gcatagtagg cagtcatatg
     1681 ttgtatcgtg aataaattgc acatagtagc tacccagcaa atgctgactt cttttctttc
     1741 tagtcttaac actccctttc tatcattttc cactcttgta cttgttctca acatttactt
     1801 ggtagtgaca tacctttcct gcagggcctg cccattgttg acagacaagt agtttattgt
     1861 ggcttatctt cggatactgg gttattaggt ttttttccct ccaattattt tttattcatt
     1921 cattcattcc taatatagcc attatgagtt aggtatagtt ctaggcatta tttataatct
     1981 tgacagtaaa cttaagagat gtgtattatc ttcatttaca aataggaaaa ctaagtcttg
     2041 acagtattag gtaatttgcc caaagtaaca aagcaaagct gatcagtggt agaaggtttt
     2101 catgattcat tgtcagcgtc atttacatag aggtgaagtt tcttgtgcag ggttacacac
     2161 ctagcaagta gcagagctgg cattcaaatt gaggcctgat gtgctaatgt aaaaggaaaa
     2221 aaacaaactt tttttccttt tgtactttca cactcaacat ggaacacttc tgtgacaagg
     2281 tgtgtgagtt tgtttccccc acacaccaag caattctcca gcagacacca actgggtatc
     2341 ctataattca attcatttct gacattacct ggaaatagtg tcagatccca caggttgagg
     2401 gctccatctc ccaagactgc caacactttt gatgccagtc atgagtctgg gccacccata
     2461 ctcctgactg accaactaca aactagagtc cttgatttcc tttggttcag ttaatttcct
     2521 aggacagctc acacaactca gggaaacacg ttcactggtt tattataaaa gatacagctg
     2581 aacagcccaa tgaaggaggt acacagggca aggttgaggg agagggagag ggtgtggagc
     2641 ttccatgcct tctctgggct cacactctct cagcacctca ccaatctgga aggaaccact
     2701 gtgtcctgct gccttcatta gcatttagtg agcaccaatt gtttgtcagg tgctgaacta
     2761 tgtactggca atacaaaaat gggaaagata tgacccctgt tttggggggg tacatagtgc
     2821 actgaactaa attgctgtct ataataatct attgggtgga aaagtaggaa gttcattcca
     2881 gttctgcaag gtgtctgctt tcagaatgga gcccactttg gtggtggcaa ggcctggctg
     2941 agttctttca aagcaacatc caacttctct ttgctctttg acttcctcca gattctctcc
     3001 tgtgctggag gtaataaggg ttgcacttcc ctctttactt acactggtga aacatgcagt
     3061 gaggattcaa ggtcaaaatt gggtcaaagt taaactaaca cagtctcctg ccccacttac
     3121 ctgtcctcta accccatcca ccagccctaa gtggtcatca catgatcaga agtcagctgg
     3181 gggaagagca gggatagata gagcaattaa ggagcctctt ttggggtaag ccacatgaca
     3241 ggggctttgc aacagtcttt catgagtggc cgaaatcccc cactcactgc aaatgtgctt
     3301 ctcaaagatg caggattttt taataaacag aggaaatgat aaattatgtt gtaattccac
     3361 tccaaaggca tttggttctt aacagccaaa tcctctgtag tctttaaata aacaaaaact
     3421 tatttggtga acagacaggg aggaaaatga aagattttct gtacttgggt tccacatgtc
     3481 aacaatttct ctcctgtgtg tatgttggcc aaagctgtat catgataaat gaataaagga
     3541 aaacctcgag cccagggcag tcagacagct cagcaaatgg ctttcagatg attactcaat
     3601 ttcagttgac atttatcaaa gagtttctct gtgactctgt gtcagggctg tagtggtgac
     3661 agggacaaga atgagactac ctgtttggat gtgctgagaa tgagtaatgc tggagcaatt
     3721 cattctccat ctcttctctc ctacagcctc tctcaaaaag aaacactggg tatggaaatt
     3781 ctcacacttg ccagaagaat taaccacctc ttctattcag tttaatgact ttcaaagtcc
     3841 tatgaaatat tcaggccaga gagaatttga gaattttttt cctgtctggt gtgtgtgtgt
     3901 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gttggaaatt acaaaataag
     3961 ttggaatgca gaaagttaaa aaaaaaatct caccaggccc tactccttta aaattaggta
     4021 tatatctttc cagacctcct ttctctgtgc atatatgtgt gtgtgtgtgt gtgtgttcgt
     4081 ttcattttgt tttgcctttg agacagtgtc tcactctgtc gcccatcatg cagtggcaca
     4141 atcatagctc actgcagcct tgaactcttt ggctcatgca attctcccac ctcagcctcc
     4201 tgagtagctg ggactgcagc tatgcaccac cacacctggc tttttttttt ttgctttgtt
     4261 tcctaggctg gtctcaaaca cctggcctca agcggtcctt ctgcctcagc ctcccaaagc
     4321 gctgggatta caagtgtgag ccactctgcc tggccatata taattttttt tttttttttt
     4381 ttgcaatgga gtcttgctct gtcgcccacg ctggagtgca ttggcgcaat ctcggctcac
     4441 tgcaacctct gcctccctgg ttcaagcagt tttccctgct tcagcctcct gagtagctgg
     4501 gattacaggc gcccgccacc acacccagct aattttttat ttttttttag tagagatggg
     4561 gtttcaccat gttggccagg ctggtcttga actcctgact caggtgatcc acctgtctca
     4621 gcctcccaaa ttgctgggat tacaggcatg aaccactgcg cccggccata taatttttta
     4681 gaaggagaga ggggaactat tcatattctg atgtttgctt ttttttctta ccctgtaaca
     4741 atcatggtca tctgtccatg tgtgtacata atagatacaa tttattattt cattgtgata
     4801 cagtattcca taatatgggt ctaccataac ttaattcact attagcccat gacactattt
     4861 aaaaattatt tcacatgcat tggagaatgt ttaaagatga tttttttttt taagaaaaaa
     4921 gaaaggcccc tattctaaga ttatctgata aggtctgctc tttctccttc aacctcaggc
     4981 taacatttat tgaatgccta cagtcggcca ggttccatgc tagacccttt acacgtgttc
     5041 ccacattgaa acttctgatg aatccttgtt aatgtaggtg ttatgattat ctgtgttttt
     5101 ctgtgaaaaa aacaggttca gaaaggatat ttaacttgcc caaggtcaca caggtagtaa
     5161 gtgctagaat gaaatttcaa acccacatct gaccttgaag taggtcctta atcagtgtgc
     5221 tttggttccc aagtcttcgc tgaacactta ctcaaaaggg acctcgcgct gttctggacc
     5281 tggacggtga tgtgagagaa gcagaagtct tgctttcata aaacacatag caagctgttc
     5341 ctaattaatg actgcaagta aaatagcata gtacctggca cattgtaaac accaaagaaa
     5401 tggtagctct tatggagaca gtaaagatta catattaatc ccccacctgt ttggccaact
     5461 tctctagaat atgtcagaga gcaagcaaaa tgttcaccaa gcagtgaata atgataataa
     5521 taacaacaat aacagtattg acagatcagt aactaatatt tgcttggtgc tttttagttt
     5581 tcaaactgct tctacattct ttgttgcatt gaaaccactc agtgaaactt tgcggttaga
     5641 caaagagtga tcataaaagc ttgtatcctc aaaagccact ttgaggcttt cttttgccct
     5701 aatagacggt catttttcat gatgtgcatt tgaaatgata tgttccctat tttgggggta
     5761 caaattttga tatgtatgca ggaaatgtag cttatagagt atgttgttta ggttttccat
     5821 tattttttga ccctttgatg agtcttggac tgaaagtggt gtgtttcaga cccctgctat
     5881 taacatttct gttccttctt gcattttctg ctttatgaaa atggattctg ctttgtgaaa
     5941 gtactgctgt gttgttttat acatagcgct ccagacgctt acatcttctt tatttcattc
     6001 agtgctctta gtctgaattt tatcttgttg tatatcagga ttacagctct tgcttgcttt
     6061 ttgtttacat ttgctttccc catttcttga tttttaagct ttcagaatca ctattttagg
     6121 tgtttctttt tttttttgga attgggtttt gttcgatgaa ccattctgaa actccttttt
     6181 tcttattttt atttttaatt gtaaaatata cataaaattt actctcttaa ccatgttttt
     6241 tttttagaca gggtcttgct ctgtcactca ggctggattt cagtggcatg atctcagctc
     6301 actgtagcct tgacaacctg ggctgaagca atcctcccac ctcagcctcc tgagtagctg
     6361 ggattacagc cacgtgcacc gccacacctg gctaactttt aaaattattt gtagagacaa
     6421 ggtcttggct atgttgccca ggctgatctc aaactcctga gctcaggtga ttctcctacc
     6481 ttggcctccc aaagtgctgg gattacaggc ttgagcttct gtgcccagcc ttaaccattt
     6541 taagtgtaca gttcagtagc atttagtaca tttacattgt tgtacaacca tcctccagaa
     6601 ctctttacca tgcagaactg acgctctata cccattacac aaccccagtt cccccttctc
     6661 agcccccagc aaccaccatt tatgaattta taaatttgat taggaacatc atgttaagtg
     6721 gaatcataca ggatttgtct ttttgtgact agtttatttc actgaagcat gatgtcctta
     6781 aagtatatcc atattgtagc atgtatcaga atttcctttc taaggctgag taatctgttg
     6841 tatatatgaa taagtatata taaagctcct tttaacagac acttaaattc atttacattt
     6901 atttataata gtgatatgtt tagtagaacc catttacttt ttctttattt tcagtattaa
     6961 cataagtgat ttggtgtttt agccctaagc agataaagga ttagagatta gaatgaggaa
     7021 gagggttgct tcacatagac gaagttcagt ttttgtggaa taaacaaata agtgattatc
     7081 tctgtctgcc tctttgtctc tctctctctc tctttttttt ttaataagag atgaggggtc
     7141 tccctatgtt gcccaggctg gacttgaact cctgggttca agtgatcctg gaactgcaga
     7201 tatgcacagc tgtgcccgaa gatccctttc tcttacattc ctttcttctg gtctgtgttt
     7261 cagtccatcc tacgctgtta agttagaaca acctgaaaca gcttttatca tgtcactctt
     7321 ctctcagaaa ccttcattgg ttccctgttg cctgctgaat tatgtaaact cttcaccctg
     7381 gcattcagta acttctgcaa tctgggcctg atgtattcct ttaattgtga tgcatctctt
     7441 acaactaccc tgataccacc agtgcttaag cgaaacggaa ttattagctt ttctgaaagc
     7501 taataatttt gtgctgcttt ctctcacctt caagcctttt cttaggctat tttctttgcc
     7561 tagtgtatgc tcccttaaac cacttcccca ccagcaggca aaacttacct agtcttataa
     7621 gcccttcttg gtcccatgtt cttccttaca ctaactcatt cattcattat ccatcccgat
     7681 gcttctcctg attcattcat tcattatcca taccgatgcc tctcctgatc cctaaacctg
     7741 cctatgccta ttaagcgctt ggatttgcct caagcatctg acgttcagct acccacctgc
     7801 gtttcctctc tcaggaaaag cctcaccatc cacccaggtg ccaagccagg acactgggag
     7861 ccacttttgc ctccctgtcc ctctcccctc atccaataag tcaccaagtc ctgtcaattc
     7921 tcccttcaac agacctttcc tttccttcct gggcacaccc ttgagtggtg ctcaccaggg
     7981 aacagatgtg gcaaatgttc caggctggcc tcacccgggt ctctagacac ttggctcaga
     8041 ggcagaacat ggctaaccct agataagcct tgtgtgaatt cctggcaaga aagtcccctg
     8101 ctgtcttagt ctgtgcattc tctgactagc acagtatgtg gcgagagcag gtgttgagga
     8161 aatgtttgtc taattctaag gggcctgcca ggccttgata caaatggcgt cacatttaca
     8221 tgaataaccc agagtttctc taattatcct cacctgcctg cccaggacct tcaacctcat
     8281 ctgctaagtc ggcctcaggc gtttctagac atcttaaggg accgcatggc atccaaggcc
     8341 actggcatct actgtactcc cctcccaccg gcgcttccct ttgctagcat ctgcagccac
     8401 aagtgacact taagggagat ttacctccac tactgggagt gttagtgtct gtgacaaaag
     8461 tgctggttgc tgggctgagg gggtctgagc atgctgggtg gaagccctga agaggaaggg
     8521 agaaggtctc tgcccacatc ccacatttgc cgtgcatccc tgagttccaa agcagcctcg
     8581 ctaggctcta gagcccctaa atctccaaag acagcagctc ctccgcgagc tagctgcccc
     8641 tgaccgaggc tcacagcttg cctaggggag ggcagggcaa aagtccctgc tattctcttc
     8701 ctttcctggc tcaggccttg atccaaatag ctcccttctt ccaagacact actccccagg
     8761 cctcctcttc ctgataataa tagtggctaa caagttttac tagattttag gcaactcaag
     8821 gagctagctg ttattttgtt cattctacaa gtgaggaagg aagcacagac agtggttaag
     8881 ttacatgtcc agagacacac agagggccta gtgcagtagc tcatgcctgg aatctcagca
     8941 ctttgggtgg ctgaggtggg cgagtcactt gaggtcagga gttcgggacc agcctggcca
     9001 acgtggtgaa accccatctc tactaaaaat aaaaaattta gccaggtgtg gtggcgggcg
     9061 cctgtaatcc cagctacttc ggaggctgaa gcaggagaat cacttgaacc caggaggcag
     9121 aggttgcagt gagccaagat tgcaccactg cactgcagca tgggcaacag agcgagactc
     9181 tgtctccaca cagaggaaca cttggcatca gggagtctgt tgtctgaccc gcaactaggc
     9241 agcctcatac attattttca ctaagagacc agtggggctg gaaagggact tgtgaggtgc
     9301 taagtagcca gtgagagctt ctgggtcctg tcgcagtttt gccctttctt ccctcaaaaa
     9361 aaggtctctc cacttctctg tgggaaaggc ttgggcaagt tctctggttt ccaatgaggc
     9421 cagagaacct gtgctgatcc ctaacatctt cacccatcgg gggcaaaata agtaaaataa
     9481 aagtaatgta gcaaatgttt ttataaaagt gttaatttaa attaacggac tattacctat
     9541 tcttagagct ttctcttttg tttttggcat taaaatatcc tttctattat aaaa
//
