LOCUS       NM_053035               4210 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein S33 (MRPS33),
            transcript variant 2, mRNA.
ACCESSION   NM_053035
VERSION     NM_053035.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   3  (bases 1 to 4210)
  AUTHORS   Amunts,A., Brown,A., Toots,J., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Ribosome. The structure of the human mitochondrial ribosome
  JOURNAL   Science 348 (6230), 95-98 (2015)
   PUBMED   25838379
REFERENCE   4  (bases 1 to 4210)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4210)
  AUTHORS   Tsuritani,K., Irie,T., Yamashita,R., Sakakibara,Y., Wakaguri,H.,
            Kanai,A., Mizushima-Sugano,J., Sugano,S., Nakai,K. and Suzuki,Y.
  TITLE     Distinct class of putative 'non-conserved' promoters in humans:
            comparative studies of alternative promoters of human and mouse
            genes
  JOURNAL   Genome Res 17 (7), 1005-1014 (2007)
   PUBMED   17567985
REFERENCE   6  (bases 1 to 4210)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   7  (bases 1 to 4210)
  AUTHORS   Cavdar Koc,E., Burkhart,W., Blackburn,K., Moseley,A. and
            Spremulli,L.L.
  TITLE     The small subunit of the mammalian mitochondrial ribosome.
            Identification of the full complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (22), 19363-19374 (2001)
   PUBMED   11279123
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ420818.1, BC105793.1,
            W95986.1, AI305281.1, AA743808.1 and AC006006.2.
            
            On Nov 22, 2018 this sequence version replaced NM_053035.2.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. The 28S subunit of the mammalian mitoribosome may play a
            crucial and characteristic role in translation initiation. This
            gene encodes a 28S subunit protein that is one of the more highly
            conserved mitochondrial ribosomal proteins among mammals,
            Drosophila and C. elegans. Splice variants that differ in the 5'
            UTR have been found for this gene; all variants encode the same
            protein. Pseudogenes corresponding to this gene are found on
            chromosomes 1q, 4p, 4q, and 20q [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) uses a different segment for
            its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same
            protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BI088186.1, SRR1803612.83510.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2145313
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000324787.10/
                                                  ENSP00000320567.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 BQ420818.1         30-34
            6-339               BC105793.1         4-337
            340-498             W95986.1           312-470
            499-636             AI305281.1         24-161              c
            637-659             AA743808.1         3-25                c
            660-4210            AC006006.2         121732-125282       c
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q34"
     gene            1..4210
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /note="mitochondrial ribosomal protein S33"
                     /db_xref="GeneID:51650"
                     /db_xref="HGNC:HGNC:16634"
                     /db_xref="MIM:611993"
     exon            1..42
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /inference="alignment:Splign:2.1.0"
     exon            43..284
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..390
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /note="28S ribosomal protein S33, mitochondrial;
                     mitochondrial small ribosomal subunit protein mS33"
                     /codon_start=1
                     /product="small ribosomal subunit protein mS33"
                     /protein_id="NP_444263.1"
                     /db_xref="CCDS:CCDS5864.1"
                     /db_xref="GeneID:51650"
                     /db_xref="HGNC:HGNC:16634"
                     /db_xref="MIM:611993"
                     /translation="MSSLSEYAFRMSRLSARLFGEVTRPTNSKSMKVVKLFSELPLAK
                     KKETYDWYPNHHTYAELMQTLRFLGLYRDEHQDFMDEQKRLKKLRGKEKPKKGEGKRA
                     AKRK"
     exon            285..4210
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      635..640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /note="hexamer: AATAAA"
     polyA_site      652
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /note="major polyA site"
     regulatory      4189..4194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
                     /note="hexamer: AATAAA"
     polyA_site      4210
                     /gene="MRPS33"
                     /gene_synonym="CGI-139; MRP-S33; mS33; PTD003; S33mt"
ORIGIN      
        1 cttccgggac gagggcgcgt gggtgaggaa ggtcaggtct aggaactcta actccttgcc
       61 actcaagaaa tgtcctccct ttcagaatat gccttccgca tgtctcgtct cagtgcccgg
      121 ctatttggtg aagtcaccag gcctactaat tccaagtcta tgaaagtggt gaaactgttt
      181 agtgaactgc ccttggccaa gaagaaggag acttatgatt ggtatccaaa tcaccacact
      241 tacgctgaac tcatgcagac gctccgattt cttggactct acagagatga gcatcaggat
      301 tttatggatg agcaaaaacg actaaagaag cttcgtggaa aggagaaacc aaagaaagga
      361 gaagggaaaa gagcagcaaa aaggaaatag tgttggtccc tcaagaggga gactttcttc
      421 ctcagtggcg gagagaagaa agtgcattta ttgtctttcc acctattgga ggaatgtcat
      481 cttcctaaat gaagtttatt tggaggaaca cagtcatctc cttggtgaaa tctaatccgg
      541 ttacattgtg gctggtttct tgaacacatt ctaactgtgc aaaattatct tggccttggc
      601 cgtgtaatgt gaggtttacc tgattctcta atgaaataaa tacctaagtt attgtttgat
      661 gatttgtttg gggtgggatg ggatgctggg ggaatggagg ggaaacctca ttaagtgagc
      721 atcctatagt gtcctcattt ctcccttctt gcttactgac tcacccagcc ctaaacttct
      781 ttcatcttgg gcctttcagc cactgtctcc accaccaagc tcacaaactt tgtgtgtgtc
      841 ttcacctact cctttgagaa atctattaag atgaatttgt agagtaagta tcttttaaaa
      901 ggaagtattg aatacctgct ctgtacaaga tattggggcc ctggtaatac acgtggttag
      961 caagagaaat actccctacc tcatgtaagt ttcagtcttg tgggagggac taatattaca
     1021 taattacata caattgtatt acatgctaca aaggaaaaag ccagacttct ataagagcat
     1081 ggatggatgg atgaatgcag agagagatag ccagaagccc aaatcacatt agggagttta
     1141 aagaaagctt ccctaaagaa atgatattta agccaggcgc tgttgctcac acctgtaatc
     1201 ccaccacttt gggaggccga ggtgggtgga tcacaaggtc aagagatcga gaccattttg
     1261 gccaacatgg tgaaacccca cctactaaaa atgcaaaaat tagctgggcg tcgtggcatg
     1321 cacctgtagt ctcagctact cgggatgctg aggcaggaga atcgcttgaa cccgggaggc
     1381 aaaggttgca gtgagttgag atcacgccac tgcactccag cctggcaaca gagcaagact
     1441 acatctcaaa aaaaaaatga tatttaagct gaaccttgaa gaataaataa gaggttgcca
     1501 aaaggaaaat ggtgagaaag gggtttgagg cagagagtac tatttgcaaa gccctaaggt
     1561 atgaagaaat tgtctgttca agaaacactg agtttgttca tggttggctg taagaaaaca
     1621 gaggccagat catgaagggc tttggaaatt ctgtggaaaa gcttgaacat aactagaagt
     1681 tctatgaggg atgactgttg tatagatttg agtagctcgc tgctggtttt attgtattcc
     1741 agcaatgagt ttccaaagaa attgtgaagg cttggccggg cacagtggct cacgcctgta
     1801 atcccagcac tttgggaggt cgaggcgggt ggatcacgag gtcaggggat cgagaccatc
     1861 ctggctaaca tggtgaaacc ccgtctctac taaaaaatgc aaaaaagtta gccgggcgtg
     1921 gtggtgggtg cctgtagtcc cagctacttg ggaggctgag gcaggagaat ggcagggagg
     1981 cggagcttgc agtaagctga gatggtgcca ctgcactcca gcctgggcaa caaagcgaga
     2041 ctctgtctca aaaaaaaaat tgtgaaggct tctctgattt ttttttccaa cagttgttct
     2101 gaatctttag catgtccagt aattttttaa taacttttta ttattgtagt aaaaacataa
     2161 aattaatgat cttaaccata tttaagtgta caatagtgtc aatattcaaa catattattc
     2221 aaaaaataat caaaatataa aagcatttta gtgcgatagt atttattgga gtatgtatag
     2281 cagctactcc agccacagtt ttctttctct gtaacactca atgattcctg aagtagaaag
     2341 attcccagta catatgttta aatgaatgct atttttaaag atacctaatc cagatggtat
     2401 ggctagggcc tattttatag cctgtttaaa aaaataaaat aaaataaaat tgggtggttt
     2461 gtatgtggta gaagtgaatt ccagcaaaag gagaattcat tcatttagac cttttttttt
     2521 gtttgagacg gagtctcact ctcaccagct ggagtgcagt ggcgcaatct cagctcactg
     2581 caagctccac ctcccgggtt cacgccattc tcctgcctca gcctcccaag tagctgggac
     2641 tacaggcgcc caccaccacg cccggctaat attttgtatt tttagtagag aaggtgtctc
     2701 actgtgttag ccaggatggt ctcaacctct tgacctcctg atccacccac ctcagactcc
     2761 caaagtgctg gaattacagg catgagccac cacacccagc ccatttagaa ctttttactg
     2821 agcacctgcc atgtgctcga cacgtgatag ttgtagagga tacagagaca aatagactta
     2881 gtccctaaga ctgtgtgaat gagcatgtgt gtattacaaa gtattggcaa gatctcagaa
     2941 acgtgcttag aaaagtgcag atgcactgtt attcttggcc aatgcagctg agccaggtgt
     3001 aggtggatga ctaccccagg atgttgagct gagaaacagg atctcagcct gaacccaatg
     3061 tagctcagca gatgtttgaa ttatgtgatc tccagctttt gggaaagatc tgaatgtaag
     3121 aggaacttaa gagaattagc atgatatttt aaagagaata actgcattac attctaacag
     3181 cagacctgag tcggaaaaca agtaaagaat cgatgtattg gtttgccgat ggaagagctt
     3241 cagtgagatc tcagaccctg accctgcagt gagccttggc aatgattgac agggaagttt
     3301 gtagataacg cagaagtaga ttagtgtgcc actgctccag gctgaaaatc agaaatgaaa
     3361 gctaaaatac atttgtacat aagctatacc ttcaattgat agagcaagct gtgtgactca
     3421 aaacagcctg tacagaagta cgatggacca gctggaaggt atcaatagaa gataaggaaa
     3481 agttaactct tgacaccagg gaagtcaaca aatttcagga caacagtgac caatggttaa
     3541 gatggtgact aagtgtaatc agaaaaaaat ctttaactcc aagtttagac tagccagttt
     3601 gtttaattgg atttctctgg tcaaaaccta aactattggt gctttctaac ctgtggggat
     3661 cagtgtgcat agaccatttt gccctcgatt tcagcataac atagtaaaaa gactgggttt
     3721 tagattcaaa agtcatgggt ttgtgataca gtgtacttac tgattatgtg acgtcagtta
     3781 cttctctgaa cctctttcct tctctataaa atcataatgc ttcactggat taatgaaata
     3841 agatatttgt gaaaagaact ctgtaaatta taaaaagtta aaaactttta catatagcac
     3901 attatgtgac tagtaagtac tgctgtgtct tactagcgca attttctgtg gcagcattga
     3961 cacgtatctc ctaaaaatat aaagttcccc acgtttacaa gcagcatgat aggttcagga
     4021 agaggaggta gatgagaggt aacatcttat tatttatgta attatgggct ccataagttt
     4081 attttttaag caccatatat gtgtgtgttt caaaataaac ataaggcatc atatatttaa
     4141 acatatataa ccataatgct tgtatttata aatcattttc caagaaagaa taaatcaaaa
     4201 tttgaaattc
//
