LOCUS       NM_014018                838 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein S28 (MRPS28), mRNA.
ACCESSION   NM_014018
VERSION     NM_014018.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 838)
  AUTHORS   Pulman,J., Ruzzenente,B., Bianchi,L., Rio,M., Boddaert,N.,
            Munnich,A., Rotig,A. and Metodiev,M.D.
  TITLE     Mutations in the MRPS28 gene encoding the small mitoribosomal
            subunit protein bS1m in a patient with intrauterine growth
            retardation, craniofacial dysmorphism and multisystemic involvement
  JOURNAL   Hum Mol Genet 28 (9), 1445-1462 (2019)
   PUBMED   30566640
  REMARK    GeneRIF: MRPS28 joins the ranks of genes encoding mitoribosomal
            proteins linked to impaired mitoribosomal biogenesis and function
            in mitochondrial disease.
REFERENCE   3  (bases 1 to 838)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   4  (bases 1 to 838)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   5  (bases 1 to 838)
  AUTHORS   Amunts,A., Brown,A., Toots,J., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Ribosome. The structure of the human mitochondrial ribosome
  JOURNAL   Science 348 (6230), 95-98 (2015)
   PUBMED   25838379
REFERENCE   6  (bases 1 to 838)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   7  (bases 1 to 838)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
REFERENCE   8  (bases 1 to 838)
  AUTHORS   Cavdar Koc,E., Burkhart,W., Blackburn,K., Moseley,A. and
            Spremulli,L.L.
  TITLE     The small subunit of the mammalian mitochondrial ribosome.
            Identification of the full complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (22), 19363-19374 (2001)
   PUBMED   11279123
REFERENCE   9  (bases 1 to 838)
  AUTHORS   Koc,E.C., Burkhart,W., Blackburn,K., Moseley,A., Koc,H. and
            Spremulli,L.L.
  TITLE     A proteomics approach to the identification of mammalian
            mitochondrial small subunit ribosomal proteins
  JOURNAL   J Biol Chem 275 (42), 32585-32591 (2000)
   PUBMED   10938081
REFERENCE   10 (bases 1 to 838)
  AUTHORS   O'Brien,T.W., Liu,J., Sylvester,J.E., Mougey,E.B.,
            Fischel-Ghodsian,N., Thiede,B., Wittmann-Liebold,B. and Graack,H.R.
  TITLE     Mammalian mitochondrial ribosomal proteins (4). Amino acid
            sequencing, characterization, and identification of corresponding
            gene sequences
  JOURNAL   J Biol Chem 275 (24), 18153-18159 (2000)
   PUBMED   10751423
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC010150.1 and AC036214.9.
            
            On Nov 22, 2018 this sequence version replaced NM_014018.2.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 28S subunit protein that has been
            called mitochondrial ribosomal protein S35 in the literature.
            [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.75156.1,
                                           SRR5189658.31207.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000276585.9/
                                                  ENSP00000276585.4
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-695               BC010150.1         13-707
            696-838             AC036214.9         59222-59364
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q21.13"
     gene            1..838
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /note="mitochondrial ribosomal protein S28"
                     /db_xref="GeneID:28957"
                     /db_xref="HGNC:HGNC:14513"
                     /db_xref="MIM:611990"
     exon            1..224
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..575
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /note="mitochondrial 28S ribosomal protein S35; 28S
                     ribosomal protein S28, mitochondrial; S28mt; S35mt; 28S
                     ribosomal protein S35, mitochondrial; mitochondrial small
                     ribosomal subunit protein bS1m"
                     /codon_start=1
                     /product="small ribosomal subunit protein bS1m"
                     /protein_id="NP_054737.1"
                     /db_xref="CCDS:CCDS6226.1"
                     /db_xref="GeneID:28957"
                     /db_xref="HGNC:HGNC:14513"
                     /db_xref="MIM:611990"
                     /translation="MAALCRTRAVAAESHFLRVFLFFRPFRGVGTESGSESGSSNAKE
                     PKTRAGGFASALERHSELLQKVEPLQKGSPKNVESFASMLRHSPLTQMGPAKDKLVIG
                     RIFHIVENDLYIDFGGKFHCVCRRPEVDGEKYQKGTRVRLRLLDLELTSRFLGATTDT
                     TVLEANAVLLGIQESKDSRSKEEHHEK"
     exon            225..406
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /inference="alignment:Splign:2.1.0"
     exon            407..838
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /inference="alignment:Splign:2.1.0"
     regulatory      677..682
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /note="hexamer: AATAAA"
     polyA_site      700
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
     regulatory      813..818
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /note="hexamer: AATAAA"
     polyA_site      838
                     /gene="MRPS28"
                     /gene_synonym="COXPD47; HSPC007; MRP-S28; MRP-S35; MRPS35"
                     /note="major polyA site"
ORIGIN      
        1 gtaaagaagt catggcggcg ctgtgtcgga cccgtgctgt ggctgccgag agccattttc
       61 tgcgagtgtt tctcttcttc aggccctttc ggggtgtagg cactgagagt ggatccgaaa
      121 gtggtagttc caatgccaag gagcctaaga cgcgcgcagg cggtttcgcg agcgcgttgg
      181 agcggcactc ggagcttcta cagaaggtgg agcccctaca gaagggttct ccaaaaaatg
      241 tggaatcctt tgcatctatg ctgagacatt ctcctcttac acagatggga cctgcaaagg
      301 ataaactggt cattggacgg atctttcata ttgtggagaa tgatctgtac atagattttg
      361 gtggaaagtt tcattgtgta tgtagaagac cagaagtgga tggagagaaa taccagaaag
      421 gaaccagggt ccggttgcgg ctattagatc ttgaacttac gtctaggttc ctgggagcaa
      481 caacagatac aactgtacta gaggctaatg cagttctctt gggaatccag gagagtaaag
      541 actcaagatc gaaagaagaa catcatgaaa aataaatgaa ctttgcttag tggattgact
      601 cctttgctga agtcagttat tcatcaagaa tgcaattaga ctaattgtga ataaatgatt
      661 gaatgaagat ataataaata aaagctataa ttatagataa ctcttattag aattttcttt
      721 agcaatattc ccacccccca ccccttgttt tgctcttaat gtttttttcc tttggtgggg
      781 atagtataca ctgtactaag aaattgtcat tcaataaata cgttttgagt gctgtcta
//
