LOCUS       NM_015084               2770 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein S27 (MRPS27),
            transcript variant 2, mRNA.
ACCESSION   NM_015084
VERSION     NM_015084.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Zhao,M., Liu,A., Mo,L., Wan,G. and Lu,F.
  TITLE     The value of RPS15 and MRPS27 in ischemic stroke
  JOURNAL   Medicine (Baltimore) 102 (33), e34706 (2023)
   PUBMED   37603533
  REMARK    GeneRIF: The value of RPS15 and MRPS27 in ischemic stroke.
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Chevalier,B., Baatallah,N., Najm,M., Castanier,S., Jung,V.,
            Pranke,I., Golec,A., Stoven,V., Marullo,S., Antigny,F.,
            Guerrera,I.C., Sermet-Gaudelus,I., Edelman,A. and Hinzpeter,A.
  TITLE     Differential CFTR-Interactome Proximity Labeling Procedures
            Identify Enrichment in Multiple SLC Transporters
  JOURNAL   Int J Mol Sci 23 (16), 8937 (2022)
   PUBMED   36012204
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2770)
  AUTHORS   Lim,S.H., Snider,J., Birimberg-Schwartz,L., Ip,W., Serralha,J.C.,
            Botelho,H.M., Lopes-Pacheco,M., Pinto,M.C., Moutaoufik,M.T.,
            Zilocchi,M., Laselva,O., Esmaeili,M., Kotlyar,M., Lyakisheva,A.,
            Tang,P., Lopez Vazquez,L., Akula,I., Aboualizadeh,F., Wong,V.,
            Grozavu,I., Opacak-Bernardi,T., Yao,Z., Mendoza,M., Babu,M.,
            Jurisica,I., Gonska,T., Bear,C.E., Amaral,M.D. and Stagljar,I.
  TITLE     CFTR interactome mapping using the mammalian membrane two-hybrid
            high-throughput screening system
  JOURNAL   Mol Syst Biol 18 (2), e10629 (2022)
   PUBMED   35156780
REFERENCE   4  (bases 1 to 2770)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   5  (bases 1 to 2770)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2770)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2770)
  AUTHORS   Tang,T., Zheng,B., Chen,S.H., Murphy,A.N., Kudlicka,K., Zhou,H. and
            Farquhar,M.G.
  TITLE     hNOA1 interacts with complex I and DAP3 and regulates mitochondrial
            respiration and apoptosis
  JOURNAL   J Biol Chem 284 (8), 5414-5424 (2009)
   PUBMED   19103604
REFERENCE   8  (bases 1 to 2770)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   9  (bases 1 to 2770)
  AUTHORS   Cavdar Koc,E., Burkhart,W., Blackburn,K., Moseley,A. and
            Spremulli,L.L.
  TITLE     The small subunit of the mammalian mitochondrial ribosome.
            Identification of the full complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (22), 19363-19374 (2001)
   PUBMED   11279123
REFERENCE   10 (bases 1 to 2770)
  AUTHORS   Koc,E.C., Burkhart,W., Blackburn,K., Koc,H., Moseley,A. and
            Spremulli,L.L.
  TITLE     Identification of four proteins from the small subunit of the
            mammalian mitochondrial ribosome using a proteomics approach
  JOURNAL   Protein Sci 10 (3), 471-481 (2001)
   PUBMED   11344316
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC341760.1, AK025417.1,
            BM995930.1 and AC012609.7.
            
            On Jun 2, 2019 this sequence version replaced NM_015084.2.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 28S subunit protein that may be a
            functional partner of the death associated protein 3 (DAP3).
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Nov 2013].
            
            Transcript Variant: This variant (2) lacks an exon in the coding
            region, compared to variant 1. The encoded isoform (2) is shorter
            compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC064902.1, AK025417.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000261413.10/
                                                  ENSP00000261413.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 DC341760.1         18-18
            2-2645              AK025417.1         2-2645
            2646-2764           BM995930.1         17-135              c
            2765-2770           AC012609.7         88555-88560
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.2"
     gene            1..2770
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /note="mitochondrial ribosomal protein S27"
                     /db_xref="GeneID:23107"
                     /db_xref="HGNC:HGNC:14512"
                     /db_xref="MIM:611989"
     exon            1..92
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..1264
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mitochondrial 28S ribosomal protein S27; mitochondrial
                     small ribosomal subunit protein mS27; 28S ribosomal
                     protein S27, mitochondrial"
                     /codon_start=1
                     /product="small ribosomal subunit protein mS27 isoform 2"
                     /protein_id="NP_055899.2"
                     /db_xref="CCDS:CCDS4013.1"
                     /db_xref="GeneID:23107"
                     /db_xref="HGNC:HGNC:14512"
                     /db_xref="MIM:611989"
                     /translation="MAASIVRRGMLLARQVVLPQLSPAGKRYLLSSAYVDSHKWEARE
                     KEHYCLADLASLMDKTFERKLPVSSLTISRLIDNISSREEIDHAEYYLYKFRHSPNCW
                     YLRNWTIHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENYKDA
                     LSVVFEVMMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLLLPGLKQKNS
                     VGFSSQLYGYALLGKVELQQGLRAVYHNMPLIWKPGYLDRALQVMEKVAASPEDIKLC
                     REALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERF
                     KALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQR
                     EQQQREQAKQEYQAQKAAKASA"
     exon            93..170
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            171..241
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            242..300
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            301..415
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            416..494
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            495..610
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            611..713
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            714..856
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1024
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..2770
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2627..2632
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /note="hexamer: AATAAA"
     polyA_site      2645
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /note="major polyA site"
     regulatory      2741..2746
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
                     /note="hexamer: ATTAAA"
     polyA_site      2770
                     /gene="MRPS27"
                     /gene_synonym="MRP-S27; mS27; S27mt"
ORIGIN      
        1 ccttttggta cgctccaaga tggctgcctc catagtgcgg cgcgggatgc tcctggcgcg
       61 gcaagtggtt cttcctcagc tctctcctgc aggtaaaaga tacctgcttt cttcagccta
      121 tgtagacagc cacaaatggg aagcaagaga aaaagaacat tactgtcttg ctgatcttgc
      181 atctttaatg gataaaacat ttgagagaaa gttgcctgtt agttctttaa caatatcacg
      241 gcttatagac aacatttcct ctcgggaaga gatagatcat gcagagtatt acctttacaa
      301 gtttcgacac agccccaact gctggtacct gagaaactgg actatccaca cctggattag
      361 gcagtgtcta aaatatgatg cacaagacaa agccctatat acccttgtaa ataaggttca
      421 atatggaatt tttccagata actttacatt caatttactg atggattctt tcataaagaa
      481 agaaaattac aaagatgctt tatctgtggt ttttgaggtc atgatgcaag aagcctttga
      541 agtgccttcc acccaacttc tctccctcta tgttttattt cattgcctgg caaagaagac
      601 agacttcagt tgggaagagg agaggaactt tggtgcatcc cttttgcttc caggcctaaa
      661 acaaaagaac tcagtgggtt tcagttccca gttgtatggc tatgcacttc ttgggaaggt
      721 ggagttgcag caagggctac gggctgtgta ccacaacatg cctctgatat ggaaaccagg
      781 ctaccttgac agagcccttc aagtgatgga gaaagtggct gcctccccag aagacataaa
      841 gctgtgtaga gaagcgctcg atgtgctggg tgcagtgctg aaggctctga cttcagctga
      901 tggggcttca gaggagcagt cccaaaatga tgaagacaac caggggtcag aaaaactggt
      961 ggagcagtta gacatcgagg aaacagagca gtccaagctt cctcaatacc tggaacgatt
     1021 taaggcctta cattctaagc ttcaagctct gggcaaaatt gagtcagaag gtcttttaag
     1081 tctgaccacc cagcttgtca aggaaaaact ctccacctgt gaagcagagg acatcgccac
     1141 ctatgagcag aatctgcagc agtggcatct agaccttgta cagttgatcc agagagaaca
     1201 gcaacagagg gagcaagcga agcaggagta ccaggctcag aaagcagcaa aggcatctgc
     1261 ctaatagggt cccccagggc cccacctgtc tcacaagaac ttcactcaac cccgtgccag
     1321 gactcagcag tggcctggac aacagcctca gcttcctcta cccatcttct tttcttaaag
     1381 caggctatgt gccctacatg gcaaggcacc atgactgccc atcgagatgc caagaagggc
     1441 tatggaacta tgcaggtggc tagtggtcag actgaagtca ccagctgaat accttaagga
     1501 ggactcttga ggctcataat ggagttcctg gggcacaggg attagttatg agcattaaag
     1561 ttcctaagac ccagtgacag tactgggaga aaccaaggct gaagagtcag gttgaagcac
     1621 aggcttttgt tttttgttgt ttttgttttt tgagacagag tctcattctg tcacccaggc
     1681 tggagtgcag tggcgcaatc ttggctcact gcaacctccg actcccaggt tcaagcaatt
     1741 ctcctgcctc ggcctccttt agtaggtggg attacaggtg cataccacca cacctttcta
     1801 atttttgcat ttttagtaga gatggggttt caccatgttg gtcaggctgg tctcaacctc
     1861 ctgacctcaa gtgatctgcc cgcctcagcc tcccaaagtg ctgggatgat aggcgtgagc
     1921 caccacaccc ggccaagcac aggcttttga atggtctccc tctccccagc ccaggtacat
     1981 gaggccaagg tgaactgtgc atcctgagac cttgtgtccc tgagtctcct ttctgagtgc
     2041 aagctgcact gtgagctggc tgtgggatac tcacacattc ccacattctc ctttctgcca
     2101 catctcgccc ttctggacct gcactgggag aaattcagga caggagtgaa ctcaaggcca
     2161 tgaactcact ggatttccat ttttaggcac ccatagggat gtttttagga tgtaagtttc
     2221 tgaggagaaa gctagctcta gcatgaagct tttttggatt ggcttctcca tcgtgttggg
     2281 gtaacatttt ttttccaatt ttttttttcc caaaacatcg tctcagctca tttatcaagt
     2341 aggtaaaatg agccttttgt gtacacacag aggcacatgt gcatgcacac acaacttgtg
     2401 aacacacatt tctgttaaag aagttagaaa atgagagatg ggttggggct tgaagtgcat
     2461 cagaggtatg aatgttgtaa aactgtcagg agatgtaaaa ttcctttctg aagtgtctct
     2521 tctgtgaaag ggttcagagc agattttgct tactatgtag tgttgccctt aagtacagtg
     2581 gtgtaatttt attcaagatt gtgctcttct atcaaaagcc tctggaaata aatgttccgg
     2641 gatcatgtta gtgtactctt tatctttggg gaaagggagg agggagaggg actcattatt
     2701 gttactatga ttttgaggag tgtactttaa accctccccc attaaactat gggatttatt
     2761 gtaataatga
//
