LOCUS       NM_018135               1163 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein S18A (MRPS18A),
            transcript variant 1, mRNA.
ACCESSION   NM_018135
VERSION     NM_018135.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Kovalevska,L.M., Malysheva,T.A., Kalman,S.S., Rozumenko,A.V.,
            Verbova,L.V., Rozumenko,V.D. and Kashuba,E.V.
  TITLE     Expression pattern of MRPS18 family genes in gliomas
  JOURNAL   Exp Oncol 43 (3), 204-208 (2021)
   PUBMED   34591434
  REMARK    GeneRIF: Expression pattern of MRPS18 family genes in gliomas.
REFERENCE   3  (bases 1 to 1163)
  AUTHORS   Zhou,C., Chen,Z., Peng,C., Chen,C. and Li,H.
  TITLE     Long Noncoding RNA TRIM52-AS1 Sponges miR-514a-5p to Facilitate
            Hepatocellular Carcinoma Progression Through Increasing MRPS18A
  JOURNAL   Cancer Biother Radiopharm 36 (2), 211-219 (2021)
   PUBMED   32391716
  REMARK    GeneRIF: Long Noncoding RNA TRIM52-AS1 Sponges miR-514a-5p to
            Facilitate Hepatocellular Carcinoma Progression Through Increasing
            MRPS18A.
REFERENCE   4  (bases 1 to 1163)
  AUTHORS   Brown,A., Rathore,S., Kimanius,D., Aibara,S., Bai,X.C., Rorbach,J.,
            Amunts,A. and Ramakrishnan,V.
  TITLE     Structures of the human mitochondrial ribosome in native states of
            assembly
  JOURNAL   Nat Struct Mol Biol 24 (10), 866-869 (2017)
   PUBMED   28892042
REFERENCE   5  (bases 1 to 1163)
  AUTHORS   Sorensen,K.M., Meldgaard,T., Melchjorsen,C.J., Fridriksdottir,A.J.,
            Pedersen,H., Petersen,O.W. and Kristensen,P.
  TITLE     Upregulation of Mrps18a in breast cancer identified by selecting
            phage antibody libraries on breast tissue sections
  JOURNAL   BMC Cancer 17 (1), 19 (2017)
   PUBMED   28056857
  REMARK    GeneRIF: We identified the mitochondrial protein Mrps18a as being
            upregulated in human breast cancer cells compared to normal breast
            cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1163)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   7  (bases 1 to 1163)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   8  (bases 1 to 1163)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
REFERENCE   9  (bases 1 to 1163)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Proteomic analysis of the mammalian mitochondrial ribosome.
            Identification of protein components in the 28 S small subunit
  JOURNAL   J Biol Chem 276 (35), 33181-33195 (2001)
   PUBMED   11402041
REFERENCE   10 (bases 1 to 1163)
  AUTHORS   Cavdar Koc,E., Burkhart,W., Blackburn,K., Moseley,A. and
            Spremulli,L.L.
  TITLE     The small subunit of the mammalian mitochondrial ribosome.
            Identification of the full complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (22), 19363-19374 (2001)
   PUBMED   11279123
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL136131.15, BC010364.1 and
            CA420521.1.
            
            On May 20, 2020 this sequence version replaced NM_018135.3.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 28S subunit protein that belongs to
            the ribosomal protein S18P family. The encoded protein is one of
            three that has significant sequence similarity to bacterial S18
            proteins. The primary sequences of the three human mitochondrial
            S18 proteins are no more closely related to each other than they
            are to the prokaryotic S18 proteins. A pseudogene corresponding to
            this gene is found on chromosome 3p. Alternative splicing results
            in multiple transcript variants.[provided by RefSeq, Jul 2010].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: HX787674.1, HX785533.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2152474, SAMEA2152798
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000372133.8/
                                                  ENSP00000361206.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AL136131.15        53502-53509         c
            9-1055              BC010364.1         5-1051
            1056-1140           AL136131.15        36943-37027         c
            1141-1163           CA420521.1         24-46               c
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..1163
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /note="mitochondrial ribosomal protein S18A"
                     /db_xref="GeneID:55168"
                     /db_xref="HGNC:HGNC:14515"
                     /db_xref="MIM:611981"
     exon            1..124
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /inference="alignment:Splign:2.1.0"
     CDS             13..603
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; mitochondrial ribosomal protein S18-3; 28S
                     ribosomal protein S18a, mitochondrial; 28S ribosomal
                     protein S18-3, mitochondrial; 39S ribosomal protein S18-3,
                     mitochondrial; mitochondrial large ribosomal subunit
                     protein bS18m-A; 39S ribosomal protein S18a,
                     mitochondrial; mitochondrial large ribosomal subunit
                     protein mL66; mitochondrial large ribosomal subunit
                     protein bS18a"
                     /codon_start=1
                     /product="large ribosomal subunit protein mL66 isoform 1
                     precursor"
                     /protein_id="NP_060605.1"
                     /db_xref="CCDS:CCDS4906.1"
                     /db_xref="GeneID:55168"
                     /db_xref="HGNC:HGNC:14515"
                     /db_xref="MIM:611981"
                     /translation="MAALKALVSGCGRLLRGLLAGPAATSWSRLPARGFREVVETQEG
                     KTTIIEGRITATPKESPNPPNPSGQCPICRWNLKHKYNYDDVLLLSQFIRPHGGMLPR
                     KITGLCQEEHRKIEECVKMAHRAGLLPNHRPRLPEGVVPKSKPQLNRYLTRWAPGSVK
                     PIYKKGPRWNRVRMPVGSPLLRDNVCYSRTPWKLYH"
     exon            125..156
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /inference="alignment:Splign:2.1.0"
     exon            157..264
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /inference="alignment:Splign:2.1.0"
     exon            265..388
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /inference="alignment:Splign:2.1.0"
     exon            389..458
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /inference="alignment:Splign:2.1.0"
     exon            459..1163
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      889..894
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /note="hexamer: CATAAA"
     polyA_site      911
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /note="major polyA site"
     polyA_site      1058
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
     regulatory      1144..1149
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
                     /note="hexamer: AAGAAA"
     polyA_site      1163
                     /gene="MRPS18A"
                     /gene_synonym="HumanS18b; mL66; MRP-S18-3; MRP-S18-a;
                     MRPS18-3; S18bmt; S18mt-a"
ORIGIN      
        1 aggtttttga agatggcggc cctcaaggct ctggtgtccg gctgtgggcg gcttctccgt
       61 gggctactag cgggcccggc agcgaccagc tggtctcggc ttccagctcg cgggttcagg
      121 gaagtggtgg agacccaaga agggaagaca actataattg aaggccgtat cacagcgact
      181 cccaaggaga gtccaaatcc tcctaacccc tctggccagt gccccatctg ccgttggaac
      241 ctgaagcaca agtataacta tgacgatgtt ctgctgctta gccagttcat ccggcctcat
      301 ggaggcatgc tgccccgaaa gatcacaggc ctatgccagg aagaacaccg caagatcgag
      361 gagtgtgtga agatggccca ccgagcaggt ctattaccaa atcacaggcc tcggcttcct
      421 gaaggagttg ttccgaagag caaaccccaa ctcaaccggt acctgacgcg ctgggctcct
      481 ggctccgtca agcccatcta caaaaaaggc ccccgctgga acagggtgcg catgcccgtg
      541 gggtcacccc ttctgaggga caatgtctgc tactcaagaa caccttggaa gctgtatcac
      601 tgacagagag cagtgcttcc agagttcctc ctgcacctgt gctggggagt aggaggccca
      661 ctcacaagcc cttggccaca actatactcc tgtcccaccc caccacgatg gcctggtccc
      721 tccaacatgc atggacaggg gacagtggga ctaacttcag tacccttggc ctgcacagta
      781 gcaatgctgg gagctagagg caggcagggc agttgggtcc cttgccagct gctatggggc
      841 ttaggccatg ctcagtgctg gggacaggag ttttgcccaa cgcagtgtca taaactgggt
      901 tcatgggctt acccattggg tgtgcgctca ctgcttggga agtgcagggg gtcctgggca
      961 cattgccagc tgggtgctga gcattgagtc actgatctct tgtgatgggg ccaatgagtc
     1021 aattgaattc atgggccaaa caggtcccat cctcttcatg acagctgtga gctccttact
     1081 gtgggagagc tgcagggagc caaggtgggc tgcctgacac acttgccgct ctcgtgtgaa
     1141 tccaagaaac tgcgttcctc aaa
//
