LOCUS       NM_016065               2579 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein S16 (MRPS16),
            transcript variant 1, mRNA.
ACCESSION   NM_016065
VERSION     NM_016065.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   3  (bases 1 to 2579)
  AUTHORS   Amunts,A., Brown,A., Toots,J., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Ribosome. The structure of the human mitochondrial ribosome
  JOURNAL   Science 348 (6230), 95-98 (2015)
   PUBMED   25838379
REFERENCE   4  (bases 1 to 2579)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2579)
  AUTHORS   Naz,R.K. and Dhandapani,L.
  TITLE     Identification of human sperm proteins that interact with human
            zona pellucida3 (ZP3) using yeast two-hybrid system
  JOURNAL   J Reprod Immunol 84 (1), 24-31 (2010)
   PUBMED   19945174
REFERENCE   6  (bases 1 to 2579)
  AUTHORS   Miller,C., Saada,A., Shaul,N., Shabtai,N., Ben-Shalom,E., Shaag,A.,
            Hershkovitz,E. and Elpeleg,O.
  TITLE     Defective mitochondrial translation caused by a ribosomal protein
            (MRPS16) mutation
  JOURNAL   Ann Neurol 56 (5), 734-738 (2004)
   PUBMED   15505824
  REMARK    GeneRIF: Defective mitochondrial translation caused by a ribosomal
            protein (MRPS16) mutation.
REFERENCE   7  (bases 1 to 2579)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   8  (bases 1 to 2579)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
REFERENCE   9  (bases 1 to 2579)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Proteomic analysis of the mammalian mitochondrial ribosome.
            Identification of protein components in the 28 S small subunit
  JOURNAL   J Biol Chem 276 (35), 33181-33195 (2001)
   PUBMED   11402041
REFERENCE   10 (bases 1 to 2579)
  AUTHORS   Cavdar Koc,E., Burkhart,W., Blackburn,K., Moseley,A. and
            Spremulli,L.L.
  TITLE     The small subunit of the mammalian mitochondrial ribosome.
            Identification of the full complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (22), 19363-19374 (2001)
   PUBMED   11279123
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ441549.1, AC016394.13 and
            AK025715.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 24, 2018 this sequence version replaced NM_016065.3.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 28S subunit protein that belongs to
            the ribosomal protein S16P family. The encoded protein is one of
            the most highly conserved ribosomal proteins between mammalian and
            yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32,
            22q12-q13.1) for this gene have been described. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.195063.1,
                                           SRR1803614.54310.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2152568, SAMEA2155550
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000372945.8/
                                                  ENSP00000362036.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BQ441549.1         53-78
            27-175              AC016394.13        134760-134908       c
            176-436             AC016394.13        134053-134313       c
            437-2550            AC016394.13        131168-133281       c
            2551-2579           AK025715.1         2531-2559
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.2"
     gene            1..2579
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /note="mitochondrial ribosomal protein S16"
                     /db_xref="GeneID:51021"
                     /db_xref="HGNC:HGNC:14048"
                     /db_xref="MIM:609204"
     exon            1..175
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..576
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /note="isoform 1 is encoded by transcript variant 1; 28S
                     ribosomal protein S16, mitochondrial; mitochondrial small
                     ribosomal subunit protein bS16m"
                     /codon_start=1
                     /product="small ribosomal subunit protein bS16m isoform 1"
                     /protein_id="NP_057149.1"
                     /db_xref="CCDS:CCDS7323.1"
                     /db_xref="GeneID:51021"
                     /db_xref="HGNC:HGNC:14048"
                     /db_xref="MIM:609204"
                     /translation="MVHLTTLLCKAYRGGHLTIRLALGGCTNRPFYRIVAAHNKCPRD
                     GRFVEQLGSYDPLPNSHGEKLVALNLDRIRHWIGCGAHLSKPMEKLLGLAGFFPLHPM
                     MITNAERLRRKRAREVLLASQKTDAEATDTEATET"
     exon            176..436
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /inference="alignment:Splign:2.1.0"
     exon            437..2579
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      669..674
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /note="hexamer: AATAAA"
     polyA_site      687
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /note="major polyA site"
     regulatory      2555..2560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
                     /note="hexamer: AATAAA"
     polyA_site      2579
                     /gene="MRPS16"
                     /gene_synonym="bS16m; CGI-132; COXPD2; MRP-S16; RPMS16;
                     S16mt"
ORIGIN      
        1 atccgcttcc ggagtcgagg ttttcgggct tgtaccgctt ggcggtgcgg cctggtgtcg
       61 gcttgcaggt tctttctgtg tttgttctct gccctgccaa ggccgtagag ctggtgcgtg
      121 cgggtagcgg ggctctccga ggagccgcac gccggcggca ccatggtcca cctcactact
      181 ctcctctgca aggcctaccg tgggggccac ttaaccatcc gccttgccct gggtggctgc
      241 accaatcggc cgttctaccg cattgtggct gctcacaaca agtgtcccag ggatggccgt
      301 ttcgtagagc agctgggctc ctatgatcca ttgcccaaca gtcatggaga aaaactcgtt
      361 gccctcaacc tagacaggat ccgtcattgg attggctgcg gggcccacct ctctaagcct
      421 atggaaaagc ttctgggtct tgctggcttt ttccctctgc atcctatgat gatcacaaat
      481 gctgagagac tgcgaaggaa acgggcacgt gaagtcctgt tagcttctca gaaaacagat
      541 gcagaagcta cagatacaga ggctacagaa acataaatga gctgacttta gtgagcatag
      601 cagtgggaac aaggtcaagg tccttttgaa acactgcagc gatcttaatt ttgttagatt
      661 tggagttcaa taaatggagt atcctgagtt gcccttgctc ttctggcctg gcctgcacag
      721 ggcccaggga gagatttgtt cttgtgtgac ttagagctgg gtgtgggtac taattagctt
      781 ttttcgactt tgtcttggga tagacagtgg ctatgggagg attggacttt tgagttgggc
      841 tctgggtctc ttggacaact ttacaattta ctggcttcca agacttcctg cttcaaaacc
      901 cccagccaga ctattcatgg cccattcaga tcttcatgtt catcccacaa gtgcaagaac
      961 agttaacctt tcttaattga tttttgtaat tggaggttta tattgtcttg cctaatgcat
     1021 attctctttt tttttttttt tttgagacgg agtcttgttc tgttgccagg ctggagtgcg
     1081 gtggtgcaat ctcagctcac tgcaatctcc acctcctggg ttcaagaggt tctcctgcct
     1141 cagcctcctg agtagccggg gagctacaag catgcaccac cacacccagc taattttttt
     1201 tttttttttg agaggagtct cgctctgtcg cccaggcttg agtgcagtgg cgcgatctcg
     1261 gctcactgca agctctgtct cctgggttca tgccattctc ctgcttcagc ctcccgagta
     1321 gtcccaggag tagctgggac tacaggtgcc caccaccaca cccagctaat ttttttgtat
     1381 ttttagtaga gatggggttt caccatgtta tccaggatgg ttttgatctc ctgacctcgt
     1441 gatccgcccg ccttggcctc ccaaaagtgc tgggattata ggcgtgagcc accgcccggg
     1501 caaatttttg tatttttagt agagatgggg tttcaccgtg ttggccagga tggtctcaat
     1561 ctgaccttgt gatctgccca cctcggcctc ccaaagtgct aggattactg gcgtgagcca
     1621 ccactcctag ccttaatgca tattcttaaa tatacaaagg tagatttgtt atgaaaattg
     1681 ctttggggct ctaataacct accttttaag aatgagaaac tgctgggctt aagggagttc
     1741 agtatgaatc aagattgaac cattcaaatg tggctgtgat ttctgcatat atcatagatg
     1801 ggatccttct gagaatactg gaatagggaa ttaggacacc aagccaattc agctgtgaac
     1861 cttattcttg tacttttctt tcttgctggt aattttatgg agcaggttaa gaaggctgct
     1921 ctgtgttagg ataaactgta taccaataat gttgacaacc tgtaatgagt gttgcatttt
     1981 acttcttgta tcttttcctt cctaccttga tgccagtaat ctataaggga tctttatagt
     2041 ttgaatgtat ttgaataact tcagtatact ttagttctac ttttttattt gactcacaac
     2101 cattcttagg tctcaagtat tcccatgtgt tttaaaagcc tgaagtcagt gagatgaaat
     2161 tcaacatcaa gaatttgaag taacttgtaa ggaaaaataa tataaagata ccattggggc
     2221 agtggctcac acctgtaatc tcagcacttt gggaggctga ggtggaagga tcacttgaag
     2281 ccagagtttg agaccagcct gtgcaacaca gcaagacccc gtctctacaa aaacttaaaa
     2341 aattagctgg ctgtggtgtt gctcacccat agttccagct actcgggaag ctgaggcagt
     2401 aagatcactt gagcccagga ggccgatgct gcagtgaact gtgattgttc cactacagtc
     2461 cagcctgggt gacagagaaa agaaaaagaa aacattacat aatttggcta gagcataata
     2521 atttgatttt ctggtttttg aaaatttgag ttgcaataaa aggatatttc agtgtgcga
//
