LOCUS       NM_031280                953 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens mitochondrial ribosomal protein S15 (MRPS15), mRNA.
ACCESSION   NM_031280
VERSION     NM_031280.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   David,F., Roussel,E., Froment,C., Draia-Nicolau,T., Pujol,F.,
            Burlet-Schiltz,O., Henras,A.K., Lacazette,E., Morfoisse,F.,
            Tatin,F., Diaz,J.J., Catez,F., Garmy-Susini,B. and Prats,A.C.
  TITLE     Mitochondrial Ribosomal Protein MRPS15 Is a Component of Cytosolic
            Ribosomes and Regulates Translation in Stressed Cardiomyocytes
  JOURNAL   Int J Mol Sci 25 (6), 3250 (2024)
   PUBMED   38542224
  REMARK    GeneRIF: Mitochondrial Ribosomal Protein MRPS15 Is a Component of
            Cytosolic Ribosomes and Regulates Translation in Stressed
            Cardiomyocytes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 953)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 953)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   5  (bases 1 to 953)
  AUTHORS   Lee,S.Y., Kang,M.G., Park,J.S., Lee,G., Ting,A.Y. and Rhee,H.W.
  TITLE     APEX Fingerprinting Reveals the Subcellular Localization of
            Proteins of Interest
  JOURNAL   Cell Rep 15 (8), 1837-1847 (2016)
   PUBMED   27184847
REFERENCE   6  (bases 1 to 953)
  AUTHORS   Davies,S.M., Rackham,O., Shearwood,A.M., Hamilton,K.L., Narsai,R.,
            Whelan,J. and Filipovska,A.
  TITLE     Pentatricopeptide repeat domain protein 3 associates with the
            mitochondrial small ribosomal subunit and regulates translation
  JOURNAL   FEBS Lett 583 (12), 1853-1858 (2009)
   PUBMED   19427859
REFERENCE   7  (bases 1 to 953)
  AUTHORS   Zhang,Z. and Gerstein,M.
  TITLE     Identification and characterization of over 100 mitochondrial
            ribosomal protein pseudogenes in the human genome
  JOURNAL   Genomics 81 (5), 468-480 (2003)
   PUBMED   12706105
REFERENCE   8  (bases 1 to 953)
  AUTHORS   Kenmochi,N., Suzuki,T., Uechi,T., Magoori,M., Kuniba,M., Higa,S.,
            Watanabe,K. and Tanaka,T.
  TITLE     The human mitochondrial ribosomal protein genes: mapping of 54
            genes to the chromosomes and implications for human disorders
  JOURNAL   Genomics 77 (1-2), 65-70 (2001)
   PUBMED   11543634
REFERENCE   9  (bases 1 to 953)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Proteomic analysis of the mammalian mitochondrial ribosome.
            Identification of protein components in the 28 S small subunit
  JOURNAL   J Biol Chem 276 (35), 33181-33195 (2001)
   PUBMED   11402041
REFERENCE   10 (bases 1 to 953)
  AUTHORS   Cavdar Koc,E., Burkhart,W., Blackburn,K., Moseley,A. and
            Spremulli,L.L.
  TITLE     The small subunit of the mammalian mitochondrial ribosome.
            Identification of the full complement of ribosomal proteins present
  JOURNAL   J Biol Chem 276 (22), 19363-19374 (2001)
   PUBMED   11279123
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM048518.1, AK315441.1,
            BU927793.1, BC031336.1, CF123360.1 and AC119675.2.
            
            On Nov 23, 2018 this sequence version replaced NM_031280.3.
            
            Summary: Mammalian mitochondrial ribosomal proteins are encoded by
            nuclear genes and help in protein synthesis within the
            mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
            small 28S subunit and a large 39S subunit. They have an estimated
            75% protein to rRNA composition compared to prokaryotic ribosomes,
            where this ratio is reversed. Another difference between mammalian
            mitoribosomes and prokaryotic ribosomes is that the latter contain
            a 5S rRNA. Among different species, the proteins comprising the
            mitoribosome differ greatly in sequence, and sometimes in
            biochemical properties, which prevents easy recognition by sequence
            homology. This gene encodes a 28S subunit protein that belongs to
            the ribosomal protein S15P family. The encoded protein is more than
            two times the size of its E. coli counterpart, with the 12S rRNA
            binding sites conserved. Between human and mouse, the encoded
            protein is the least conserved among small subunit ribosomal
            proteins. Pseudogenes corresponding to this gene are found on
            chromosomes 15q and 19q. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK315441.1, BC031336.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000373116.6/
                                                  ENSP00000362208.5
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 BM048518.1         56-61
            7-315               AK315441.1         1-309
            316-722             BU927793.1         221-627
            723-897             BC031336.1         693-867
            898-910             CF123360.1         358-370
            911-953             AC119675.2         89647-89689         c
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.3"
     gene            1..953
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /note="mitochondrial ribosomal protein S15"
                     /db_xref="GeneID:64960"
                     /db_xref="HGNC:HGNC:14504"
                     /db_xref="MIM:611979"
     exon            1..239
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..883
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /note="28S ribosomal protein S15, mitochondrial; MRP-S15;
                     mitochondrial small ribosomal subunit protein uS15m"
                     /codon_start=1
                     /product="small ribosomal subunit protein uS15m"
                     /protein_id="NP_112570.2"
                     /db_xref="CCDS:CCDS411.1"
                     /db_xref="GeneID:64960"
                     /db_xref="HGNC:HGNC:14504"
                     /db_xref="MIM:611979"
                     /translation="MLRVAWRTLSLIRTRAVTQVLVPGLPGGGSAKFPFNQWGLQPRS
                     LLLQAARGYVVRKPAQSRLDDDPPPSTLLKDYQNVPGIEKVDDVVKRLLSLEMANKKE
                     MLKIKQEQFMKKIVANPEDTRSLEARIIALSVKIRSYEEHLEKHRKDKAHKRYLLMSI
                     DQRKKMLKNLRNTNYDVFEKICWGLGIEYTFPPLYYRRAHRRFVTKKALCIRVFQETQ
                     KLKKRRRALKAAAAAQKQAKRRNPDSPAKAIPKTLKDSQ"
     exon            240..284
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     exon            285..360
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     exon            361..409
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     exon            410..494
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     exon            495..553
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     exon            554..745
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     exon            746..953
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /inference="alignment:Splign:2.1.0"
     regulatory      879..884
                     /regulatory_class="polyA_signal_sequence"
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /note="hexamer: AATAAA"
     polyA_site      897
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
                     /note="major polyA site"
     polyA_site      953
                     /gene="MRPS15"
                     /gene_synonym="DC37; MPR-S15; RPMS15; S15mt; uS15m"
ORIGIN      
        1 gcctcgattg gtcgatcctg ggccagcatg gcggcgccca tgtaacccgg tccgtgccgc
       61 aaagcgaacg gcggccgcgg cgcgggcccc gcgggggtta gaggtcacca tgctgagggt
      121 cgcgtggagg acgctgagtt tgattcggac ccgggcagtt acccaggtcc tagtacccgg
      181 gctgccgggc ggtgggagcg ccaagtttcc tttcaaccag tggggcctgc agcctcgaag
      241 tctcctcctc caggccgcgc gcggatatgt cgtccggaaa ccagcccagt ctaggctgga
      301 tgatgaccca cctccctcta cgctgctcaa agactaccag aatgtccctg gaattgagaa
      361 ggttgatgat gtcgtgaaaa gactcttgtc tttggaaatg gccaacaaga aggagatgct
      421 aaaaatcaag caagaacagt ttatgaagaa gattgttgca aacccagagg acaccagatc
      481 cctggaggct cgaattattg ccttgtctgt caagatccgc agttatgaag aacacttgga
      541 gaaacatcga aaggacaaag cccacaaacg ctatctgcta atgagcattg accagaggaa
      601 aaagatgctc aaaaacctcc gtaacaccaa ctatgatgtc tttgagaaga tatgctgggg
      661 gctgggaatt gagtacacct tcccccctct gtattaccga agagcccacc gccgattcgt
      721 gaccaagaag gctctgtgca ttcgggtttt ccaggagact caaaagctga agaagcgaag
      781 aagagcctta aaggctgcag cagcagccca aaaacaagca aagcggagga acccagacag
      841 ccctgccaaa gccataccaa agacactcaa agacagccaa taaattctgt tcaatcattt
      901 ctttctgtct tgaagaatga taggagagat gatggggctc tttttggcct gaa
//
